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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 138, 213 |
| AcsI | RAATTY | 1 cut(s) | 312 |
| AfaI | GTAC | 1 cut(s) | 112 |
| AluBI | AGCT | 2 cut(s) | 88, 130 |
| AluI | AGCT | 2 cut(s) | 88, 130 |
| Alw21I | GWGCWC | 1 cut(s) | 90 |
| ApeKI | GCWGC | 1 cut(s) | 174 |
| ApoI | RAATTY | 1 cut(s) | 312 |
| AsuHPI | GGTGA | 5 cut(s) | 91, 130, 152, 173, 200 |
| BanII | GRGCYC | 2 cut(s) | 90, 310 |
| Bbv12I | GWGCWC | 1 cut(s) | 90 |
| BbvI | GCAGC | 1 cut(s) | 161 |
| BccI | CCATC | 5 cut(s) | 76, 115, 152, 158, 313 |
| BfaI | CTAG | 1 cut(s) | 237 |
| BfmI | CTRYAG | 1 cut(s) | 201 |
| BisI | GCNGC | 1 cut(s) | 175 |
| BlsI | GCNGC | 1 cut(s) | 176 |
| BmiI | GGNNCC | 2 cut(s) | 255, 328 |
| BmsI | GCATC | 1 cut(s) | 328 |
| BseGI | GGATG | 2 cut(s) | 324, 343 |
| BseRI | GAGGAG | 1 cut(s) | 221 |
| BseXI | GCAGC | 1 cut(s) | 161 |
| BseYI | CCCAGC | 1 cut(s) | 52 |
| BsiHKAI | GWGCWC | 1 cut(s) | 90 |
| Bsp1286I | GDGCHC | 2 cut(s) | 90, 310 |
| BspACI | CCGC | 2 cut(s) | 138, 213 |
| BspLI | GGNNCC | 2 cut(s) | 255, 328 |
| Bst4CI | ACNGT | 5 cut(s) | 70, 76, 115, 182, 194 |
| BstDEI | CTNAG | 1 cut(s) | 342 |
| BstF5I | GGATG | 2 cut(s) | 324, 343 |
| BstSFI | CTRYAG | 1 cut(s) | 201 |
| BstV1I | GCAGC | 1 cut(s) | 161 |
| BtsCI | GGATG | 2 cut(s) | 324, 343 |
| BtsIMutI | CAGTG | 2 cut(s) | 18, 81 |
| Csp6I | GTAC | 1 cut(s) | 111 |
| CviAII | CATG | 1 cut(s) | 99 |
| CviJI | RGCY | 7 cut(s) | 88, 130, 174, 236, 270, 308, 327 |
| CviKI_1 | RGCY | 7 cut(s) | 88, 130, 174, 236, 270, 308, 327 |
| CviQI | GTAC | 1 cut(s) | 111 |
| DdeI | CTNAG | 1 cut(s) | 342 |
| Ecl136II | GAGCTC | 1 cut(s) | 88 |
| Eco24I | GRGCYC | 2 cut(s) | 90, 310 |
| Eco53kI | GAGCTC | 1 cut(s) | 88 |
| EcoICRI | GAGCTC | 1 cut(s) | 88 |
| EcoT38I | GRGCYC | 2 cut(s) | 90, 310 |
| FaeI | CATG | 1 cut(s) | 102 |
| FaiI | YATR | 2 cut(s) | 100, 291 |
| FatI | CATG | 1 cut(s) | 98 |
| Fnu4HI | GCNGC | 1 cut(s) | 175 |
| FokI | GGATG | 1 cut(s) | 331 |
| FriOI | GRGCYC | 2 cut(s) | 90, 310 |
| Fsp4HI | GCNGC | 1 cut(s) | 175 |
| FspBI | CTAG | 1 cut(s) | 237 |
| GluI | GCNGC | 1 cut(s) | 175 |
| GsaI | CCCAGC | 1 cut(s) | 56 |
| Hin1II | CATG | 1 cut(s) | 102 |
| HphI | GGTGA | 5 cut(s) | 91, 130, 152, 173, 200 |
| Hpy166II | GTNNAC | 1 cut(s) | 279 |
| Hpy8I | GTNNAC | 1 cut(s) | 279 |
| HpyCH4III | ACNGT | 5 cut(s) | 70, 76, 115, 182, 194 |
| HpyF3I | CTNAG | 1 cut(s) | 342 |
| Hsp92II | CATG | 1 cut(s) | 102 |
| LmnI | GCTCC | 2 cut(s) | 85, 332 |
| LpnPI | CCDG | 3 cut(s) | 23, 38, 116 |
| Lsp1109I | GCAGC | 1 cut(s) | 161 |
| LweI | GCATC | 1 cut(s) | 328 |
| MaeI | CTAG | 1 cut(s) | 237 |
| MaeIII | GTNAC | 4 cut(s) | 56, 70, 140, 188 |
| MfeI | CAATTG | 1 cut(s) | 61 |
| MhlI | GDGCHC | 2 cut(s) | 90, 310 |
| MluCI | AATT | 3 cut(s) | 61, 106, 312 |
| MnlI | CCTC | 3 cut(s) | 199, 202, 226 |
| MunI | CAATTG | 1 cut(s) | 61 |
| NlaIII | CATG | 1 cut(s) | 102 |
| NlaIV | GGNNCC | 2 cut(s) | 255, 328 |
| NmuCI | GTSAC | 3 cut(s) | 70, 140, 188 |
| PkrI | GCNGC | 1 cut(s) | 176 |
| Psp124BI | GAGCTC | 1 cut(s) | 90 |
| PspFI | CCCAGC | 1 cut(s) | 52 |
| PspN4I | GGNNCC | 2 cut(s) | 255, 328 |
| RsaI | GTAC | 1 cut(s) | 112 |
| RsaNI | GTAC | 1 cut(s) | 111 |
| SacI | GAGCTC | 1 cut(s) | 90 |
| SatI | GCNGC | 1 cut(s) | 175 |
| SduI | GDGCHC | 2 cut(s) | 90, 310 |
| SetI | ASST | 2 cut(s) | 90, 132 |
| SfaNI | GCATC | 1 cut(s) | 328 |
| SfcI | CTRYAG | 1 cut(s) | 201 |
| SgeI | CNNG | 8 cut(s) | 50, 65, 111, 115, 143, 249, 270, 283 |
| Sse9I | AATT | 3 cut(s) | 61, 106, 312 |
| SsiI | CCGC | 2 cut(s) | 138, 213 |
| SspMI | CTAG | 1 cut(s) | 237 |
| SstI | GAGCTC | 1 cut(s) | 90 |
| TaaI | ACNGT | 5 cut(s) | 70, 76, 115, 182, 194 |
| TaqI | TCGA | 1 cut(s) | 34 |
| TasI | AATT | 3 cut(s) | 61, 106, 312 |
| TscAI | CASTG | 2 cut(s) | 18, 81 |
| TseFI | GTSAC | 3 cut(s) | 70, 140, 188 |
| TseI | GCWGC | 1 cut(s) | 174 |
| Tsp45I | GTSAC | 3 cut(s) | 70, 140, 188 |
| TspRI | CASTG | 2 cut(s) | 18, 81 |
| XapI | RAATTY | 1 cut(s) | 312 |
| XspI | CTAG | 1 cut(s) | 237 |