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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 37, 247 |
| AgsI | TTSAA | 1 cut(s) | 16 |
| AluBI | AGCT | 3 cut(s) | 134, 387, 431 |
| AluI | AGCT | 3 cut(s) | 134, 387, 431 |
| Alw26I | GTCTC | 3 cut(s) | 167, 209, 325 |
| AoxI | GGCC | 1 cut(s) | 415 |
| ApeKI | GCWGC | 3 cut(s) | 134, 387, 431 |
| AspS9I | GGNCC | 1 cut(s) | 416 |
| BanI | GGYRCC | 2 cut(s) | 37, 247 |
| BbvI | GCAGC | 3 cut(s) | 121, 374, 418 |
| BccI | CCATC | 1 cut(s) | 25 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 402, 425, 436, 459 |
| BcoDI | GTCTC | 3 cut(s) | 167, 209, 325 |
| BfaI | CTAG | 1 cut(s) | 392 |
| BfmI | CTRYAG | 1 cut(s) | 336 |
| BisI | GCNGC | 3 cut(s) | 135, 388, 432 |
| BlsI | GCNGC | 3 cut(s) | 136, 389, 433 |
| BmgT120I | GGNCC | 1 cut(s) | 416 |
| BmiI | GGNNCC | 3 cut(s) | 39, 249, 418 |
| BmsI | GCATC | 2 cut(s) | 130, 215 |
| BsaAI | YACGTR | 1 cut(s) | 94 |
| BsaI | GGTCTC | 2 cut(s) | 167, 325 |
| BsaJI | CCNNGG | 1 cut(s) | 412 |
| Bse118I | RCCGGY | 1 cut(s) | 244 |
| BseDI | CCNNGG | 1 cut(s) | 412 |
| BseMII | CTCAG | 1 cut(s) | 204 |
| BseRI | GAGGAG | 1 cut(s) | 19 |
| BseXI | GCAGC | 3 cut(s) | 121, 374, 418 |
| BseYI | CCCAGC | 1 cut(s) | 156 |
| BsgI | GTGCAG | 1 cut(s) | 429 |
| BshFI | GGCC | 1 cut(s) | 417 |
| BshNI | GGYRCC | 2 cut(s) | 37, 247 |
| BsiSI | CCGG | 1 cut(s) | 245 |
| BslFI | GGGAC | 1 cut(s) | 96 |
| BsmAI | GTCTC | 3 cut(s) | 167, 209, 325 |
| BsmFI | GGGAC | 1 cut(s) | 96 |
| BsnI | GGCC | 1 cut(s) | 417 |
| Bso31I | GGTCTC | 2 cut(s) | 167, 325 |
| BspANI | GGCC | 1 cut(s) | 417 |
| BspCNI | CTCAG | 1 cut(s) | 205 |
| BspLI | GGNNCC | 3 cut(s) | 39, 249, 418 |
| BspMAI | CTGCAG | 1 cut(s) | 340 |
| BspT107I | GGYRCC | 2 cut(s) | 37, 247 |
| BspTNI | GGTCTC | 2 cut(s) | 167, 325 |
| BsrFI | RCCGGY | 1 cut(s) | 244 |
| BssAI | RCCGGY | 1 cut(s) | 244 |
| BssECI | CCNNGG | 1 cut(s) | 412 |
| Bst4CI | ACNGT | 2 cut(s) | 58, 88 |
| BstBAI | YACGTR | 1 cut(s) | 94 |
| BstC8I | GCNNGC | 3 cut(s) | 119, 162, 246 |
| BstDEI | CTNAG | 1 cut(s) | 213 |
| BstMAI | GTCTC | 3 cut(s) | 167, 209, 325 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 161 |
| BstNSI | RCATGY | 1 cut(s) | 222 |
| BstSFI | CTRYAG | 1 cut(s) | 336 |
| BstV1I | GCAGC | 3 cut(s) | 121, 374, 418 |
| BsuRI | GGCC | 1 cut(s) | 417 |
| Cac8I | GCNNGC | 3 cut(s) | 119, 162, 246 |
| Cfr10I | RCCGGY | 1 cut(s) | 244 |
| Cfr13I | GGNCC | 1 cut(s) | 416 |
| CviAII | CATG | 1 cut(s) | 219 |
| CviJI | RGCY | 6 cut(s) | 134, 155, 387, 417, 431, 441 |
| CviKI_1 | RGCY | 6 cut(s) | 134, 155, 387, 417, 431, 441 |
| DdeI | CTNAG | 1 cut(s) | 213 |
| Eco31I | GGTCTC | 2 cut(s) | 167, 325 |
| EcoO109I | RGGNCCY | 1 cut(s) | 416 |
| FaeI | CATG | 1 cut(s) | 222 |
| FaiI | YATR | 1 cut(s) | 220 |
| FaqI | GGGAC | 1 cut(s) | 96 |
| FatI | CATG | 1 cut(s) | 218 |
| Fnu4HI | GCNGC | 3 cut(s) | 135, 388, 432 |
| Fsp4HI | GCNGC | 3 cut(s) | 135, 388, 432 |
| FspBI | CTAG | 1 cut(s) | 392 |
| GluI | GCNGC | 3 cut(s) | 135, 388, 432 |
| GsaI | CCCAGC | 1 cut(s) | 160 |
| HaeIII | GGCC | 1 cut(s) | 417 |
| HapII | CCGG | 1 cut(s) | 245 |
| Hin1II | CATG | 1 cut(s) | 222 |
| HpaII | CCGG | 1 cut(s) | 245 |
| Hpy166II | GTNNAC | 1 cut(s) | 91 |
| Hpy8I | GTNNAC | 1 cut(s) | 91 |
| HpyCH4III | ACNGT | 2 cut(s) | 58, 88 |
| HpyCH4IV | ACGT | 2 cut(s) | 27, 93 |
| HpyCH4V | TGCA | 3 cut(s) | 121, 338, 446 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 161 |
| HpyF3I | CTNAG | 1 cut(s) | 213 |
| HpySE526I | ACGT | 2 cut(s) | 27, 93 |
| Hsp92II | CATG | 1 cut(s) | 222 |
| KroI | GCCGGC | 1 cut(s) | 244 |
| KroNI | GCCGGC | 1 cut(s) | 246 |
| Lsp1109I | GCAGC | 3 cut(s) | 121, 374, 418 |
| LweI | GCATC | 2 cut(s) | 130, 215 |
| MaeI | CTAG | 1 cut(s) | 392 |
| MaeII | ACGT | 2 cut(s) | 27, 93 |
| MnlI | CCTC | 3 cut(s) | 28, 110, 407 |
| MroNI | GCCGGC | 1 cut(s) | 244 |
| MseI | TTAA | 1 cut(s) | 83 |
| MspI | CCGG | 1 cut(s) | 245 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 161 |
| NaeI | GCCGGC | 1 cut(s) | 246 |
| NgoMIV | GCCGGC | 1 cut(s) | 244 |
| NlaIII | CATG | 1 cut(s) | 222 |
| NlaIV | GGNNCC | 3 cut(s) | 39, 249, 418 |
| NspI | RCATGY | 1 cut(s) | 222 |
| PdiI | GCCGGC | 1 cut(s) | 246 |
| PkrI | GCNGC | 3 cut(s) | 136, 389, 433 |
| Ppu21I | YACGTR | 1 cut(s) | 94 |
| PspFI | CCCAGC | 1 cut(s) | 156 |
| PspN4I | GGNNCC | 3 cut(s) | 39, 249, 418 |
| PspPI | GGNCC | 1 cut(s) | 416 |
| PstI | CTGCAG | 1 cut(s) | 340 |
| SaqAI | TTAA | 1 cut(s) | 83 |
| SatI | GCNGC | 3 cut(s) | 135, 388, 432 |
| Sau96I | GGNCC | 1 cut(s) | 416 |
| SfaNI | GCATC | 2 cut(s) | 130, 215 |
| SfcI | CTRYAG | 1 cut(s) | 336 |
| SspMI | CTAG | 1 cut(s) | 392 |
| TaaI | ACNGT | 2 cut(s) | 58, 88 |
| TaiI | ACGT | 2 cut(s) | 30, 96 |
| Tru1I | TTAA | 1 cut(s) | 83 |
| Tru9I | TTAA | 1 cut(s) | 83 |
| TseI | GCWGC | 3 cut(s) | 134, 387, 431 |
| TspGWI | ACGGA | 1 cut(s) | 98 |
| XceI | RCATGY | 1 cut(s) | 222 |
| XspI | CTAG | 1 cut(s) | 392 |