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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 8 |
| AccI | GTMKAC | 1 cut(s) | 318 |
| AciI | CCGC | 1 cut(s) | 29 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 75 |
| AluBI | AGCT | 4 cut(s) | 53, 85, 345, 389 |
| AluI | AGCT | 4 cut(s) | 53, 85, 345, 389 |
| Alw26I | GTCTC | 2 cut(s) | 283, 304 |
| AoxI | GGCC | 1 cut(s) | 373 |
| ApeKI | GCWGC | 4 cut(s) | 53, 85, 345, 389 |
| AspLEI | GCGC | 1 cut(s) | 9 |
| AspS9I | GGNCC | 1 cut(s) | 374 |
| BbvI | GCAGC | 4 cut(s) | 40, 72, 332, 376 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 360, 383, 394, 417 |
| BcoDI | GTCTC | 2 cut(s) | 283, 304 |
| BfaI | CTAG | 2 cut(s) | 174, 350 |
| BisI | GCNGC | 5 cut(s) | 29, 54, 86, 346, 390 |
| BlpI | GCTNAGC | 1 cut(s) | 183 |
| BlsI | GCNGC | 5 cut(s) | 30, 55, 87, 347, 391 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 75 |
| BmgT120I | GGNCC | 1 cut(s) | 374 |
| BmiI | GGNNCC | 1 cut(s) | 376 |
| BmsI | GCATC | 2 cut(s) | 70, 289 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 183 |
| BsaI | GGTCTC | 2 cut(s) | 283, 304 |
| BsaJI | CCNNGG | 1 cut(s) | 370 |
| BseDI | CCNNGG | 1 cut(s) | 370 |
| BseMII | CTCAG | 1 cut(s) | 174 |
| BseXI | GCAGC | 4 cut(s) | 40, 72, 332, 376 |
| BseYI | CCCAGC | 1 cut(s) | 32 |
| BsgI | GTGCAG | 1 cut(s) | 387 |
| BshFI | GGCC | 1 cut(s) | 375 |
| BsmAI | GTCTC | 2 cut(s) | 283, 304 |
| BsnI | GGCC | 1 cut(s) | 375 |
| Bso31I | GGTCTC | 2 cut(s) | 283, 304 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 183 |
| BspACI | CCGC | 1 cut(s) | 29 |
| BspANI | GGCC | 1 cut(s) | 375 |
| BspCNI | CTCAG | 1 cut(s) | 175 |
| BspLI | GGNNCC | 1 cut(s) | 376 |
| BspTNI | GGTCTC | 2 cut(s) | 283, 304 |
| BssECI | CCNNGG | 1 cut(s) | 370 |
| BstC8I | GCNNGC | 1 cut(s) | 38 |
| BstDEI | CTNAG | 1 cut(s) | 183 |
| BstHHI | GCGC | 1 cut(s) | 9 |
| BstMAI | GTCTC | 2 cut(s) | 283, 304 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 37 |
| BstV1I | GCAGC | 4 cut(s) | 40, 72, 332, 376 |
| BsuRI | GGCC | 1 cut(s) | 375 |
| Cac8I | GCNNGC | 1 cut(s) | 38 |
| CfoI | GCGC | 1 cut(s) | 9 |
| Cfr13I | GGNCC | 1 cut(s) | 374 |
| CviJI | RGCY | 6 cut(s) | 53, 85, 345, 375, 389, 399 |
| CviKI_1 | RGCY | 6 cut(s) | 53, 85, 345, 375, 389, 399 |
| DdeI | CTNAG | 1 cut(s) | 183 |
| DriI | GACNNNNNGTC | 1 cut(s) | 75 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 75 |
| Eco31I | GGTCTC | 2 cut(s) | 283, 304 |
| EcoO109I | RGGNCCY | 1 cut(s) | 374 |
| FblI | GTMKAC | 1 cut(s) | 318 |
| Fnu4HI | GCNGC | 5 cut(s) | 29, 54, 86, 346, 390 |
| Fsp4HI | GCNGC | 5 cut(s) | 29, 54, 86, 346, 390 |
| FspAI | RTGCGCAY | 1 cut(s) | 8 |
| FspBI | CTAG | 2 cut(s) | 174, 350 |
| FspI | TGCGCA | 1 cut(s) | 8 |
| GlaI | GCGC | 1 cut(s) | 8 |
| GluI | GCNGC | 5 cut(s) | 29, 54, 86, 346, 390 |
| GsaI | CCCAGC | 1 cut(s) | 36 |
| HaeIII | GGCC | 1 cut(s) | 375 |
| HhaI | GCGC | 1 cut(s) | 9 |
| Hin6I | GCGC | 1 cut(s) | 7 |
| HinP1I | GCGC | 1 cut(s) | 7 |
| HincII | GTYRAC | 1 cut(s) | 319 |
| HindII | GTYRAC | 1 cut(s) | 319 |
| Hpy166II | GTNNAC | 1 cut(s) | 319 |
| Hpy188I | TCNGA | 1 cut(s) | 15 |
| Hpy188III | TCNNGA | 1 cut(s) | 80 |
| Hpy8I | GTNNAC | 1 cut(s) | 319 |
| HpyCH4V | TGCA | 1 cut(s) | 404 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 37 |
| HpyF3I | CTNAG | 1 cut(s) | 183 |
| HspAI | GCGC | 1 cut(s) | 7 |
| Lsp1109I | GCAGC | 4 cut(s) | 40, 72, 332, 376 |
| LweI | GCATC | 2 cut(s) | 70, 289 |
| MaeI | CTAG | 2 cut(s) | 174, 350 |
| MnlI | CCTC | 1 cut(s) | 365 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 37 |
| NlaIV | GGNNCC | 1 cut(s) | 376 |
| NsbI | TGCGCA | 1 cut(s) | 8 |
| PkrI | GCNGC | 5 cut(s) | 30, 55, 87, 347, 391 |
| PspFI | CCCAGC | 1 cut(s) | 32 |
| PspN4I | GGNNCC | 1 cut(s) | 376 |
| PspPI | GGNCC | 1 cut(s) | 374 |
| SalI | GTCGAC | 1 cut(s) | 317 |
| SatI | GCNGC | 5 cut(s) | 29, 54, 86, 346, 390 |
| Sau96I | GGNCC | 1 cut(s) | 374 |
| SfaNI | GCATC | 2 cut(s) | 70, 289 |
| SsiI | CCGC | 1 cut(s) | 29 |
| SspMI | CTAG | 2 cut(s) | 174, 350 |
| TaqI | TCGA | 2 cut(s) | 79, 318 |
| TauI | GCSGC | 1 cut(s) | 31 |
| TseI | GCWGC | 4 cut(s) | 53, 85, 345, 389 |
| XmiI | GTMKAC | 1 cut(s) | 318 |
| XspI | CTAG | 2 cut(s) | 174, 350 |