Rorug02G0168200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000981
Physical Location & Seq
Forward (+)
15105571 .. 15106997
1427 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0168200.1

Sequence Viewer

Length: 1071 bp
ATGATTAACGCAGGGGAGGTGTTTGCAGGGGCTGCTGCTGGAGGACCAGTTGGACCTGTGTTTGATGTCCTTAGAGAAGTGGTAGAGAAGTCTTTCGCGTTTAATCCCACCCTCGAAAGCGTCAAATCCACGGTGGACTCTTTAACCTTATTGATCCCGCAGATAGATGATCATAACACGACATTGGGTGGTAACCGAGCAGGGGAAGTAGAAAAGCTCAAAGAAATAATGGAGGAGGGGAAAAGGCTCATTGAAGAGCTGCTGGAGGTTAATGAGTGGAGCTGCTTGAAGGCTCATTACACCGACCAACTTGTTGAGTTGGATGAGTCTCTCAAGAGACACTTGGAGATATTGCAGGTGCAGGGAGTGATTGATGTGAAGGAGGCCTTGGTTGTGGCAAAGGATACTAATAAGGATGTGAAGGAACATAGGCATTGGCTGCAGGCTATCTCGGTCACATCAGAGGAAAACACCAACCAACTTGTCAGATTCGAAGCGACTCTTAAAACAGGACTGTCGGATCTACAGAAGGGGCAAGAAACAATGATCGACAGAATGATAAGGAGAATTAAATGGAATGGAATGGAAAACAAAGTGGGATCGTTGACAGAAGCGGCTCGTCTAGGAGCTACATTACAAGAATTGGACGATGCGGTTTTTGAGGAAACTTTAATATCGAGATATCCGGAGATCGTGATGGGACGCTTGGAAGAAATAGGTAAAAAGACAAGGGGAATATTTGAATTGATCGAATGGAAGAGGGAAGAAAACAAAGAGATTTCGTATATCTCGACTGAGGAACTAAAGAATTTCAGGGTGAAGATAGAGGAGGGGGTAAAGCTCGTCCGCAACCTTGGTGCAGTTCTTGATTGGACCGAAGCCGTTAAGTCGTTCACCAACAACAAATTCCTTCATTTGGACGAAACTCTCGAGAGACTCTACAACCGTTTAAATGGAATACACCCATTTAAATGGAGCACTGGAAAATGTGGCAGGTCATGTGGTACAACGTCAATGAAGGATTCCGATATCATTAACGATCGATTTTATCATTCAGATGATCATGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

356

Amino Acids

40.47

Weight (kDa)

5.22

Isoelectric Point (pI)

39.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPW8 PF05659 6 - 141 2.4e-21 Arabidopsis broad-spectrum mildew resistance protein RPW8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 346
Acc36I ACCTGC 2 cut(s) 346, 984
AccII CGCG 1 cut(s) 98
AccIII TCCGGA 1 cut(s) 685
AciI CCGC 4 cut(s) 158, 614, 653, 847
AclWI GGATC 3 cut(s) 148, 528, 607
AcsI RAATTY 2 cut(s) 808, 905
AfaI GTAC 1 cut(s) 1006
AfiI CCNNNNNNNGG 2 cut(s) 202, 916
AgsI TTSAA 3 cut(s) 254, 289, 743
AjuI GAANNNNNNNTTGG 4 cut(s) 371, 403, 890, 922
AluBI AGCT 5 cut(s) 217, 259, 282, 629, 841
AluI AGCT 5 cut(s) 217, 259, 282, 629, 841
Alw21I GWGCWC 1 cut(s) 980
Alw26I GTCTC 3 cut(s) 331, 333, 928
AlwI GGATC 3 cut(s) 148, 528, 607
AlwNI CAGNNNCTG 1 cut(s) 32
Ama87I CYCGRG 1 cut(s) 929
Aor13HI TCCGGA 1 cut(s) 685
AoxI GGCC 1 cut(s) 384
ApeKI GCWGC 5 cut(s) 32, 35, 259, 282, 439
ApoI RAATTY 2 cut(s) 808, 905
Asp700I GAANNNNTTC 1 cut(s) 92
AspS9I GGNCC 3 cut(s) 44, 53, 873
AsuHPI GGTGA 2 cut(s) 829, 886
AsuII TTCGAA 1 cut(s) 492
AvaI CYCGRG 1 cut(s) 929
AvaII GGWCC 3 cut(s) 44, 53, 873
BarI GAAGNNNNNNTAC 2 cut(s) 281, 313
Bbv12I GWGCWC 1 cut(s) 980
BbvI GCAGC 5 cut(s) 19, 22, 246, 269, 426
BccI CCATC 1 cut(s) 691
BceAI ACGGC 1 cut(s) 866
BciVI GTATCC 1 cut(s) 397
BclI TGATCA 2 cut(s) 169, 1060
BcoDI GTCTC 3 cut(s) 331, 333, 928
BfaI CTAG 1 cut(s) 623
BfmI CTRYAG 2 cut(s) 440, 524
BfuAI ACCTGC 2 cut(s) 346, 984
BfuI GTATCC 1 cut(s) 397
BisI GCNGC 6 cut(s) 33, 36, 260, 283, 440, 615
BlsI GCNGC 6 cut(s) 34, 37, 261, 284, 441, 616
Bme18I GGWCC 3 cut(s) 44, 53, 873
BmeT110I CYCGRG 1 cut(s) 929
BmgT120I GGNCC 3 cut(s) 44, 53, 873
BmsI GCATC 1 cut(s) 640
BpmI CTGGAG 2 cut(s) 60, 284
Bpu14I TTCGAA 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 317
Bsa29I ATCGAT 1 cut(s) 1042
BsaJI CCNNGG 3 cut(s) 129, 387, 853
BsaWI WCCGGW 1 cut(s) 685
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 916
Bse1I ACTGG 2 cut(s) 47, 985
BseAI TCCGGA 1 cut(s) 685
BseCI ATCGAT 1 cut(s) 1042
BseDI CCNNGG 3 cut(s) 129, 387, 853
BseGI GGATG 2 cut(s) 328, 421
BseLI CCNNNNNNNGG 2 cut(s) 202, 916
BseMII CTCAG 1 cut(s) 786
BseNI ACTGG 2 cut(s) 47, 985
BseRI GAGGAG 2 cut(s) 248, 842
BseXI GCAGC 5 cut(s) 19, 22, 246, 269, 426
BsgI GTGCAG 2 cut(s) 380, 879
Bsh1236I CGCG 1 cut(s) 98
Bsh1285I CGRYCG 1 cut(s) 1042
BshFI GGCC 1 cut(s) 386
BshVI ATCGAT 1 cut(s) 1042
BsiEI CGRYCG 1 cut(s) 1042
BsiHKAI GWGCWC 1 cut(s) 980
BsiHKCI CYCGRG 1 cut(s) 929
BsiSI CCGG 1 cut(s) 686
BslFI GGGAC 1 cut(s) 714
BslI CCNNNNNNNGG 2 cut(s) 202, 916
BsmAI GTCTC 3 cut(s) 331, 333, 928
BsmFI GGGAC 1 cut(s) 714
BsnI GGCC 1 cut(s) 386
BsoBI CYCGRG 1 cut(s) 929
Bsp119I TTCGAA 1 cut(s) 492
Bsp1286I GDGCHC 1 cut(s) 980
Bsp13I TCCGGA 1 cut(s) 685
Bsp143I GATC 9 cut(s) 153, 169, 520, 546, 599, 690, 747, 1039, 1060
BspACI CCGC 4 cut(s) 158, 614, 653, 847
BspANI GGCC 1 cut(s) 386
BspCNI CTCAG 1 cut(s) 787
BspDI ATCGAT 1 cut(s) 1042
BspEI TCCGGA 1 cut(s) 685
BspFNI CGCG 1 cut(s) 98
BspMAI CTGCAG 1 cut(s) 444
BspMI ACCTGC 2 cut(s) 346, 984
BspPI GGATC 3 cut(s) 148, 528, 607
BspQI GCTCTTC 1 cut(s) 249
BspT104I TTCGAA 1 cut(s) 492
BsrI ACTGG 2 cut(s) 47, 985
BssECI CCNNGG 3 cut(s) 129, 387, 853
BssMI GATC 9 cut(s) 153, 169, 520, 546, 599, 690, 747, 1039, 1060
BssT1I CCWWGG 2 cut(s) 387, 853
Bst4CI ACNGT 3 cut(s) 133, 516, 947
Bst6I CTCTTC 2 cut(s) 249, 752
BstAPI GCANNNNNTGC 2 cut(s) 32, 439
BstBI TTCGAA 1 cut(s) 492
BstC8I GCNNGC 1 cut(s) 444
BstDEI CTNAG 2 cut(s) 71, 795
BstDSI CCRYGG 1 cut(s) 129
BstEII GGTNACC 1 cut(s) 191
BstF5I GGATG 2 cut(s) 328, 421
BstFNI CGCG 1 cut(s) 98
BstKTI GATC 9 cut(s) 156, 172, 523, 549, 602, 693, 750, 1042, 1063
BstMAI GTCTC 3 cut(s) 331, 333, 928
BstMBI GATC 9 cut(s) 153, 169, 520, 546, 599, 690, 747, 1039, 1060
BstMCI CGRYCG 1 cut(s) 1042
BstMWI GCNNNNNNNGC 2 cut(s) 32, 439
BstPI GGTNACC 1 cut(s) 191
BstSFI CTRYAG 2 cut(s) 440, 524
BstUI CGCG 1 cut(s) 98
BstV1I GCAGC 5 cut(s) 19, 22, 246, 269, 426
BstX2I RGATCY 1 cut(s) 520
BstXI CCANNNNNNTGG 1 cut(s) 972
BstYI RGATCY 1 cut(s) 520
Bsu15I ATCGAT 1 cut(s) 1042
BsuI GTATCC 1 cut(s) 397
BsuRI GGCC 1 cut(s) 386
BsuTUI ATCGAT 1 cut(s) 1042
BtgI CCRYGG 1 cut(s) 129
BtsCI GGATG 2 cut(s) 328, 421
BtsIMutI CAGTG 1 cut(s) 978
BveI ACCTGC 2 cut(s) 346, 984
Cac8I GCNNGC 1 cut(s) 444
CaiI CAGNNNCTG 1 cut(s) 32
Cfr13I GGNCC 3 cut(s) 44, 53, 873
ClaI ATCGAT 1 cut(s) 1042
CseI GACGC 2 cut(s) 109, 711
Csp6I GTAC 1 cut(s) 1005
CviAII CATG 2 cut(s) 999, 1064
CviQI GTAC 1 cut(s) 1005
DdeI CTNAG 2 cut(s) 71, 795
DpnI GATC 9 cut(s) 155, 171, 522, 548, 601, 692, 749, 1041, 1062
DpnII GATC 9 cut(s) 153, 169, 520, 546, 599, 690, 747, 1039, 1060
DraI TTTAAA 2 cut(s) 951, 970
Eam1104I CTCTTC 2 cut(s) 249, 752
EarI CTCTTC 2 cut(s) 249, 752
Eco130I CCWWGG 2 cut(s) 387, 853
Eco147I AGGCCT 1 cut(s) 386
Eco32I GATATC 2 cut(s) 683, 1030
Eco47I GGWCC 3 cut(s) 44, 53, 873
Eco88I CYCGRG 1 cut(s) 929
Eco91I GGTNACC 1 cut(s) 191
EcoO65I GGTNACC 1 cut(s) 191
EcoRV GATATC 2 cut(s) 683, 1030
EcoT14I CCWWGG 2 cut(s) 387, 853
ErhI CCWWGG 2 cut(s) 387, 853
FaeI CATG 2 cut(s) 1002, 1067
FaiI YATR 6 cut(s) 174, 429, 786, 1000, 1065, 1069
FalI AAGNNNNNCTT 6 cut(s) 326, 358, 371, 403, 486, 518
FaqI GGGAC 1 cut(s) 714
FatI CATG 2 cut(s) 998, 1063
FauI CCCGC 1 cut(s) 165
FbaI TGATCA 2 cut(s) 169, 1060
Fnu4HI GCNGC 6 cut(s) 33, 36, 260, 283, 440, 615
FokI GGATG 2 cut(s) 335, 428
Fsp4HI GCNGC 6 cut(s) 33, 36, 260, 283, 440, 615
FspBI CTAG 1 cut(s) 623
GluI GCNGC 6 cut(s) 33, 36, 260, 283, 440, 615
GsuI CTGGAG 2 cut(s) 60, 284
HaeIII GGCC 1 cut(s) 386
HapII CCGG 1 cut(s) 686
HgaI GACGC 2 cut(s) 109, 711
Hin1II CATG 2 cut(s) 1002, 1067
HincII GTYRAC 1 cut(s) 606
HindII GTYRAC 1 cut(s) 606
HinfI GANTC 6 cut(s) 137, 326, 489, 499, 936, 1022
HpaII CCGG 1 cut(s) 686
HphI GGTGA 2 cut(s) 829, 886
Hpy166II GTNNAC 3 cut(s) 136, 606, 894
Hpy188I TCNGA 5 cut(s) 463, 488, 520, 1027, 1057
Hpy188III TCNNGA 8 cut(s) 334, 678, 686, 694, 790, 866, 929, 931
Hpy8I GTNNAC 3 cut(s) 136, 606, 894
HpyAV CCTTC 6 cut(s) 283, 373, 415, 523, 920, 1012
HpyCH4III ACNGT 3 cut(s) 133, 516, 947
HpyCH4IV ACGT 1 cut(s) 1010
HpyCH4V TGCA 5 cut(s) 26, 355, 361, 442, 860
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 439
HpyF3I CTNAG 2 cut(s) 71, 795
HpySE526I ACGT 1 cut(s) 1010
Hsp92II CATG 2 cut(s) 1002, 1067
Kpn2I TCCGGA 1 cut(s) 685
Ksp22I TGATCA 2 cut(s) 169, 1060
Kzo9I GATC 9 cut(s) 153, 169, 520, 546, 599, 690, 747, 1039, 1060
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 3 cut(s) 279, 626, 975
Lsp1109I GCAGC 5 cut(s) 19, 22, 246, 269, 426
LweI GCATC 1 cut(s) 640
MaeI CTAG 1 cut(s) 623
MaeII ACGT 1 cut(s) 1010
MaeIII GTNAC 2 cut(s) 191, 454
MalI GATC 9 cut(s) 155, 171, 522, 548, 601, 692, 749, 1041, 1062
MboI GATC 9 cut(s) 153, 169, 520, 546, 599, 690, 747, 1039, 1060
MboII GAAGA 5 cut(s) 266, 722, 769, 776, 832
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 1 cut(s) 980
MluCI AATT 5 cut(s) 567, 641, 743, 808, 905
MlyI GAGTC 4 cut(s) 131, 335, 493, 930
MmeI TCCRAC 3 cut(s) 31, 300, 498
MroI TCCGGA 1 cut(s) 685
MroXI GAANNNNTTC 1 cut(s) 92
MslI CAYNNNNRTG 2 cut(s) 970, 1056
MspI CCGG 1 cut(s) 686
MvnI CGCG 1 cut(s) 98
MwoI GCNNNNNNNGC 2 cut(s) 32, 439
NdeII GATC 9 cut(s) 153, 169, 520, 546, 599, 690, 747, 1039, 1060
NlaIII CATG 2 cut(s) 1002, 1067
NmuCI GTSAC 1 cut(s) 454
NspV TTCGAA 1 cut(s) 492
PaeR7I CTCGAG 1 cut(s) 929
PaqCI CACCTGC 1 cut(s) 346
PceI AGGCCT 1 cut(s) 386
PciSI GCTCTTC 1 cut(s) 249
PcsI WCGNNNNNNNCGW 2 cut(s) 788, 927
PdmI GAANNNNTTC 1 cut(s) 92
PfeI GAWTC 2 cut(s) 489, 1022
PkrI GCNGC 6 cut(s) 34, 37, 261, 284, 441, 616
Ple19I CGATCG 1 cut(s) 1042
PleI GAGTC 4 cut(s) 131, 334, 493, 930
PpsI GAGTC 4 cut(s) 131, 334, 493, 930
PspEI GGTNACC 1 cut(s) 191
PspPI GGNCC 3 cut(s) 44, 53, 873
PstI CTGCAG 1 cut(s) 444
PstNI CAGNNNCTG 1 cut(s) 32
PsuI RGATCY 1 cut(s) 520
PvuI CGATCG 1 cut(s) 1042
RsaI GTAC 1 cut(s) 1006
RsaNI GTAC 1 cut(s) 1005
RseI CAYNNNNRTG 2 cut(s) 970, 1056
SapI GCTCTTC 1 cut(s) 249
SatI GCNGC 6 cut(s) 33, 36, 260, 283, 440, 615
Sau3AI GATC 9 cut(s) 153, 169, 520, 546, 599, 690, 747, 1039, 1060
Sau96I GGNCC 3 cut(s) 44, 53, 873
SchI GAGTC 4 cut(s) 131, 335, 493, 930
SduI GDGCHC 1 cut(s) 980
SfaNI GCATC 1 cut(s) 640
SfcI CTRYAG 2 cut(s) 440, 524
Sfr274I CTCGAG 1 cut(s) 929
SfuI TTCGAA 1 cut(s) 492
SinI GGWCC 3 cut(s) 44, 53, 873
SlaI CTCGAG 1 cut(s) 929
SmiI ATTTAAAT 1 cut(s) 970
SmiMI CAYNNNNRTG 2 cut(s) 970, 1056
SmlI CTYRAG 2 cut(s) 332, 929
SmoI CTYRAG 2 cut(s) 332, 929
Sse9I AATT 5 cut(s) 567, 641, 743, 808, 905
SseBI AGGCCT 1 cut(s) 386
SsiI CCGC 4 cut(s) 158, 614, 653, 847
SspI AATATT 1 cut(s) 738
SspMI CTAG 1 cut(s) 623
StuI AGGCCT 1 cut(s) 386
StyI CCWWGG 2 cut(s) 387, 853
SwaI ATTTAAAT 1 cut(s) 970
TaaI ACNGT 3 cut(s) 133, 516, 947
TaiI ACGT 1 cut(s) 1013
TaqI TCGA 8 cut(s) 114, 492, 549, 677, 750, 791, 930, 1042
TaqII GACCGA 2 cut(s) 442, 890
TasI AATT 5 cut(s) 567, 641, 743, 808, 905
TauI GCSGC 1 cut(s) 617
TfiI GAWTC 2 cut(s) 489, 1022
TscAI CASTG 1 cut(s) 985
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 5 cut(s) 32, 35, 259, 282, 439
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 2 cut(s) 902, 1031
TspRI CASTG 1 cut(s) 985
VpaK11BI GGWCC 3 cut(s) 44, 53, 873
XapI RAATTY 2 cut(s) 808, 905
XhoI CTCGAG 1 cut(s) 929
XmnI GAANNNNTTC 1 cut(s) 92
XspI CTAG 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.