Rmu_sc0002613.1_g000004

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002613.1
OG0000981
Physical Location & Seq
Forward (+)
17516 .. 18121
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002613.1_g000004.1.cds

Sequence Viewer

Length: 549 bp
atgcctagcttactgggcggctgctctgcaaatgtcttgactctcgaaagagaggttgtccggccacttggtggctcggtaatttggagttggaagggtggtggtgctcgtctttcttctcccctatttggcagcgtcggcgattggcttagacgatgtcccatgatggcggtcagtttggctgcgaaaactcaagatttcatttcgctagtgttaagcggtgctgggcttttctctctgggcgaggttggatggggtttcgttgatggatggggtcaaggctatctggtttcagatccgagatggggctctctggaagtgatggattgctgggatcgtgcagtctctgaaggggttgtgcattgtcccggaggttgtgctagatcgggatcggacgaagatgttggagcggcggctctgacggcgaatgtcggcggcggtgcggctactttgtcgacgagactgggactgaagcacattgacagaaggtttggtggcactgacagtcaactcctgaggagactgggactggagtgtggtcaccgataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

19.06

Weight (kDa)

6.81

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 71
AccBSI CCGCTC 1 cut(s) 410
AccI GTMKAC 1 cut(s) 455
AciI CCGC 8 cut(s) 18, 170, 219, 410, 413, 435, 438, 443
AclWI GGATC 3 cut(s) 290, 342, 397
AcoI YGGCCR 1 cut(s) 62
AcuI CTGAAG 2 cut(s) 369, 491
AdeI CACNNNGTG 1 cut(s) 71
AfiI CCNNNNNNNGG 3 cut(s) 71, 128, 305
AluBI AGCT 1 cut(s) 9
AluI AGCT 1 cut(s) 9
Alw21I GWGCWC 1 cut(s) 109
Alw26I GTCTC 3 cut(s) 349, 454, 514
AlwI GGATC 3 cut(s) 290, 342, 397
AlwNI CAGNNNCTG 1 cut(s) 347
AoxI GGCC 1 cut(s) 62
ApeKI GCWGC 3 cut(s) 21, 132, 182
ArsI GACNNNNNNTTYG 2 cut(s) 473, 505
AsuC2I CCSGG 1 cut(s) 369
AsuHPI GGTGA 1 cut(s) 533
AxyI CCTNAGG 1 cut(s) 515
BanII GRGCYC 1 cut(s) 311
Bbv12I GWGCWC 1 cut(s) 109
BbvI GCAGC 3 cut(s) 8, 144, 169
BccI CCATC 6 cut(s) 160, 246, 260, 264, 297, 316
BceAI ACGGC 1 cut(s) 438
BcnI CCSGG 1 cut(s) 369
BcoDI GTCTC 3 cut(s) 349, 454, 514
BfaI CTAG 3 cut(s) 6, 209, 381
BisI GCNGC 8 cut(s) 19, 22, 133, 183, 411, 414, 436, 444
BlsI GCNGC 8 cut(s) 20, 23, 134, 184, 412, 415, 437, 445
Bme1390I CCNGG 1 cut(s) 369
BmrFI CCNGG 1 cut(s) 369
BmrI ACTGGG 3 cut(s) 23, 473, 533
BmuI ACTGGG 3 cut(s) 23, 473, 533
BpuEI CTTGAG 1 cut(s) 177
BpuMI CCSGG 1 cut(s) 369
BsaBI GATNNNNATC 1 cut(s) 388
Bsc4I CCNNNNNNNGG 3 cut(s) 71, 128, 305
Bse1I ACTGG 4 cut(s) 18, 468, 528, 534
Bse21I CCTNAGG 1 cut(s) 515
Bse8I GATNNNNATC 1 cut(s) 388
BseGI GGATG 2 cut(s) 257, 275
BseJI GATNNNNATC 1 cut(s) 388
BseLI CCNNNNNNNGG 3 cut(s) 71, 128, 305
BseMII CTCAG 1 cut(s) 506
BseNI ACTGG 4 cut(s) 18, 468, 528, 534
BseRI GAGGAG 1 cut(s) 532
BseXI GCAGC 3 cut(s) 8, 144, 169
BseYI CCCAGC 2 cut(s) 224, 330
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 109
BsiSI CCGG 2 cut(s) 61, 369
BslFI GGGAC 4 cut(s) 144, 351, 480, 540
BslI CCNNNNNNNGG 3 cut(s) 71, 128, 305
BsmAI GTCTC 3 cut(s) 349, 454, 514
BsmFI GGGAC 4 cut(s) 144, 351, 480, 540
BsnI GGCC 1 cut(s) 64
Bsp1286I GDGCHC 2 cut(s) 109, 311
Bsp143I GATC 4 cut(s) 295, 334, 383, 389
BspACI CCGC 8 cut(s) 18, 170, 219, 410, 413, 435, 438, 443
BspANI GGCC 1 cut(s) 64
BspCNI CTCAG 1 cut(s) 507
BspPI GGATC 3 cut(s) 290, 342, 397
BsrBI CCGCTC 1 cut(s) 410
BsrI ACTGG 4 cut(s) 18, 468, 528, 534
BssMI GATC 4 cut(s) 295, 334, 383, 389
Bst4CI ACNGT 1 cut(s) 506
BstDEI CTNAG 2 cut(s) 149, 515
BstEII GGTNACC 1 cut(s) 539
BstF5I GGATG 2 cut(s) 257, 275
BstKTI GATC 4 cut(s) 298, 337, 386, 392
BstMAI GTCTC 3 cut(s) 349, 454, 514
BstMBI GATC 4 cut(s) 295, 334, 383, 389
BstMWI GCNNNNNNNGC 3 cut(s) 15, 138, 422
BstPI GGTNACC 1 cut(s) 539
BstSCI CCNGG 1 cut(s) 367
BstV1I GCAGC 3 cut(s) 8, 144, 169
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
Bsu36I CCTNAGG 1 cut(s) 515
BsuRI GGCC 1 cut(s) 64
BtsCI GGATG 2 cut(s) 257, 275
BtsIMutI CAGTG 1 cut(s) 498
CaiI CAGNNNCTG 1 cut(s) 347
CseI GACGC 1 cut(s) 124
CviAII CATG 1 cut(s) 163
DdeI CTNAG 2 cut(s) 149, 515
DpnI GATC 4 cut(s) 297, 336, 385, 391
DpnII GATC 4 cut(s) 295, 334, 383, 389
DraIII CACNNNGTG 1 cut(s) 71
EaeI YGGCCR 1 cut(s) 62
Eco24I GRGCYC 1 cut(s) 311
Eco57I CTGAAG 2 cut(s) 369, 491
Eco81I CCTNAGG 1 cut(s) 515
Eco91I GGTNACC 1 cut(s) 539
EcoO65I GGTNACC 1 cut(s) 539
EcoT38I GRGCYC 1 cut(s) 311
FaeI CATG 1 cut(s) 166
FaiI YATR 1 cut(s) 164
FaqI GGGAC 4 cut(s) 144, 351, 480, 540
FatI CATG 1 cut(s) 162
FblI GTMKAC 1 cut(s) 455
Fnu4HI GCNGC 8 cut(s) 19, 22, 133, 183, 411, 414, 436, 444
FokI GGATG 2 cut(s) 264, 282
FriOI GRGCYC 1 cut(s) 311
Fsp4HI GCNGC 8 cut(s) 19, 22, 133, 183, 411, 414, 436, 444
FspBI CTAG 3 cut(s) 6, 209, 381
GluI GCNGC 8 cut(s) 19, 22, 133, 183, 411, 414, 436, 444
GsaI CCCAGC 2 cut(s) 228, 334
HaeIII GGCC 1 cut(s) 64
HapII CCGG 2 cut(s) 61, 369
HgaI GACGC 1 cut(s) 124
Hin1II CATG 1 cut(s) 166
HincII GTYRAC 2 cut(s) 456, 509
HindII GTYRAC 2 cut(s) 456, 509
HinfI GANTC 1 cut(s) 40
HpaII CCGG 2 cut(s) 61, 369
HphI GGTGA 1 cut(s) 533
Hpy166II GTNNAC 2 cut(s) 456, 509
Hpy188I TCNGA 5 cut(s) 295, 300, 349, 394, 420
Hpy188III TCNNGA 6 cut(s) 37, 44, 194, 314, 387, 514
Hpy8I GTNNAC 2 cut(s) 456, 509
Hpy99I CGWCG 2 cut(s) 140, 460
HpyAV CCTTC 3 cut(s) 88, 344, 480
HpyCH4III ACNGT 1 cut(s) 506
HpyCH4V TGCA 3 cut(s) 29, 341, 361
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 138, 422
HpyF3I CTNAG 2 cut(s) 149, 515
Hsp92II CATG 1 cut(s) 166
Kzo9I GATC 4 cut(s) 295, 334, 383, 389
LmnI GCTCC 1 cut(s) 407
Lsp1109I GCAGC 3 cut(s) 8, 144, 169
MaeI CTAG 3 cut(s) 6, 209, 381
MaeIII GTNAC 1 cut(s) 539
MalI GATC 4 cut(s) 297, 336, 385, 391
MbiI CCGCTC 1 cut(s) 410
MboI GATC 4 cut(s) 295, 334, 383, 389
MboII GAAGA 2 cut(s) 108, 410
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 2 cut(s) 109, 311
MluCI AATT 1 cut(s) 81
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 3 cut(s) 71, 229, 385
MnlI CCTC 4 cut(s) 46, 238, 365, 510
MseI TTAA 1 cut(s) 215
MspI CCGG 2 cut(s) 61, 369
MspR9I CCNGG 1 cut(s) 369
MwoI GCNNNNNNNGC 3 cut(s) 15, 138, 422
NciI CCSGG 1 cut(s) 369
NdeII GATC 4 cut(s) 295, 334, 383, 389
NlaIII CATG 1 cut(s) 166
NmuCI GTSAC 1 cut(s) 539
PflFI GACNNNGTC 1 cut(s) 156
PflMI CCANNNNNTGG 1 cut(s) 71
PfoI TCCNGGA 1 cut(s) 367
PkrI GCNGC 8 cut(s) 20, 23, 134, 184, 412, 415, 437, 445
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
PspEI GGTNACC 1 cut(s) 539
PspFI CCCAGC 2 cut(s) 224, 330
PstNI CAGNNNCTG 1 cut(s) 347
PsuI RGATCY 1 cut(s) 295
PsyI GACNNNGTC 1 cut(s) 156
SalI GTCGAC 1 cut(s) 454
SaqAI TTAA 1 cut(s) 215
SatI GCNGC 8 cut(s) 19, 22, 133, 183, 411, 414, 436, 444
Sau3AI GATC 4 cut(s) 295, 334, 383, 389
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 1 cut(s) 369
SduI GDGCHC 2 cut(s) 109, 311
SetI ASST 5 cut(s) 11, 57, 249, 376, 491
SmlI CTYRAG 1 cut(s) 192
SmoI CTYRAG 1 cut(s) 192
Sse9I AATT 1 cut(s) 81
SsiI CCGC 8 cut(s) 18, 170, 219, 410, 413, 435, 438, 443
SspMI CTAG 3 cut(s) 6, 209, 381
StyD4I CCNGG 1 cut(s) 367
TaaI ACNGT 1 cut(s) 506
TaqI TCGA 2 cut(s) 45, 455
TasI AATT 1 cut(s) 81
TauI GCSGC 5 cut(s) 21, 413, 416, 438, 446
Tru1I TTAA 1 cut(s) 215
Tru9I TTAA 1 cut(s) 215
TscAI CASTG 1 cut(s) 505
TseFI GTSAC 1 cut(s) 539
TseI GCWGC 3 cut(s) 21, 132, 182
Tsp45I GTSAC 1 cut(s) 539
TspDTI ATGAA 1 cut(s) 190
TspRI CASTG 1 cut(s) 505
Tth111I GACNNNGTC 1 cut(s) 156
Van91I CCANNNNNTGG 1 cut(s) 71
XmiI GTMKAC 1 cut(s) 455
XspI CTAG 3 cut(s) 6, 209, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.