RLG00000021424

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000981
Physical Location & Seq
Reverse (-)
75314208 .. 75315564
1357 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021424

Sequence Viewer

Length: 819 bp
ATGACGCTTTCTCTTTCTCTCGGGAGAGCAACCTTAAGGTCGCACCTGACCTTGATTGGCTGTGGTGGCACTACAAAATTCCTGACTCTAGACAGAGAGGTTTCTCAGCCACATTGTGGCTCTGCATCTTGGATTTGGAAGGGCGGCGATGTCTCTCTTTCTTCTCCCTTCCTTGGTGACGCCGGTGGTTGGCCTCGACGTCCCTTTAATATATGCTCGGATCGGTTTGGTCTTCTGGATGGATCGGAGATTCTGATGGCAGTCATTTTGGTTGAGATGACGCGAGTTTCCTTGACTTCAAGCTCGTCGGCGATAGGCGAAGGGCTTTTCGTCGCCGTGGTGTTTGGGTTAGGTGATGTGGGACGGGGACTCAGTGATGGCTGGGTTGTTCAGGCTGGAGTGGGCTCAGATCCTCTCAGATGGGTTTGGTCGATTTGTATGGTGGCCAAGGACAACGTAATCGATGGCGAACCAATTTCCCCTTCGGTGGCAAAGATTGCAGTTGGGCTACAATTACATTGTGCCAGTGGTGATGGTGATCTTTCTCCTCTGTTGGATTGCGGCGGCGATGGAAAAGGCTATGGCGGCAGTGGTAAAGGAGATCCTGCTGAGGACAGCGGCTCTTGGGTGGCTAGGTATGCCAGGGTTTGCTACTGGGCTCTTAGACTGTTGGGCCTGGAGTGGACCTTTGCCTGTGGTTGGGGCCTTTGGGCTTGCTTTTGGACGGTGGACTTCCTGTGTGATGCATGGGTCTCTAGCTGGGCTAAGTTTAATCGGGATATGTTCAGTGGCAAATTTCATAATTTGATGAAACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

273

Amino Acids

29.13

Weight (kDa)

5.55

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 202
AccB7I CCANNNNNTGG 1 cut(s) 116
AccII CGCG 1 cut(s) 283
AciI CCGC 5 cut(s) 144, 561, 564, 585, 618
AclWI GGATC 4 cut(s) 228, 250, 404, 596
AcoI YGGCCR 1 cut(s) 444
AcsI RAATTY 2 cut(s) 77, 794
AcyI GRCGYC 2 cut(s) 180, 199
AdeI CACNNNGTG 1 cut(s) 116
AfiI CCNNNNNNNGG 5 cut(s) 116, 173, 189, 487, 699
AflII CTTAAG 1 cut(s) 34
AgsI TTSAA 1 cut(s) 300
AjnI CCWGG 2 cut(s) 641, 675
AjuI GAANNNNNNNTTGG 2 cut(s) 466, 498
AluBI AGCT 2 cut(s) 303, 759
AluI AGCT 2 cut(s) 303, 759
Alw26I GTCTC 2 cut(s) 157, 757
AlwI GGATC 4 cut(s) 228, 250, 404, 596
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 4 cut(s) 191, 444, 673, 703
ApoI RAATTY 2 cut(s) 77, 794
AspS9I GGNCC 3 cut(s) 673, 684, 703
AsuHPI GGTGA 4 cut(s) 188, 365, 542, 548
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 1 cut(s) 684
BalI TGGCCA 1 cut(s) 446
BanII GRGCYC 2 cut(s) 407, 661
BbsI GAAGAC 1 cut(s) 224
BbvCI CCTCAGC 1 cut(s) 609
BccI CCATC 7 cut(s) 233, 250, 371, 414, 458, 527, 563
BceAI ACGGC 1 cut(s) 320
BciT130I CCWGG 2 cut(s) 643, 677
BcoDI GTCTC 2 cut(s) 157, 757
BfaI CTAG 3 cut(s) 89, 633, 756
BfrI CTTAAG 1 cut(s) 34
BisI GCNGC 5 cut(s) 145, 562, 565, 586, 619
BlsI GCNGC 5 cut(s) 146, 563, 566, 587, 620
Bme1390I CCNGG 2 cut(s) 643, 677
Bme18I GGWCC 1 cut(s) 684
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 3 cut(s) 673, 684, 703
BmiI GGNNCC 1 cut(s) 704
BmrFI CCNGG 2 cut(s) 643, 677
BmrI ACTGGG 1 cut(s) 664
BmsI GCATC 2 cut(s) 134, 733
BmuI ACTGGG 1 cut(s) 664
BpiI GAAGAC 1 cut(s) 224
BpmI CTGGAG 2 cut(s) 417, 698
Bpu10I CCTNAGC 1 cut(s) 609
Bsa29I ATCGAT 1 cut(s) 462
BsaBI GATNNNNATC 1 cut(s) 537
BsaHI GRCGYC 2 cut(s) 180, 199
BsaI GGTCTC 1 cut(s) 757
BsaJI CCNNGG 4 cut(s) 172, 336, 447, 642
Bsc4I CCNNNNNNNGG 5 cut(s) 116, 173, 189, 487, 699
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 2 cut(s) 525, 659
Bse8I GATNNNNATC 1 cut(s) 537
BseBI CCWGG 2 cut(s) 643, 677
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 4 cut(s) 172, 336, 447, 642
BseGI GGATG 1 cut(s) 244
BseJI GATNNNNATC 1 cut(s) 537
BseLI CCNNNNNNNGG 5 cut(s) 116, 173, 189, 487, 699
BseMII CTCAG 5 cut(s) 119, 385, 420, 430, 600
BseNI ACTGG 2 cut(s) 525, 659
BseRI GAGGAG 1 cut(s) 537
BseYI CCCAGC 2 cut(s) 381, 759
Bsh1236I CGCG 1 cut(s) 283
BshFI GGCC 4 cut(s) 193, 446, 675, 705
BshVI ATCGAT 1 cut(s) 462
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 1 cut(s) 183
BslFI GGGAC 3 cut(s) 186, 375, 381
BslI CCNNNNNNNGG 5 cut(s) 116, 173, 189, 487, 699
BsmAI GTCTC 2 cut(s) 157, 757
BsmFI GGGAC 3 cut(s) 186, 375, 381
BsnI GGCC 4 cut(s) 193, 446, 675, 705
Bso31I GGTCTC 1 cut(s) 757
BsoBI CYCGRG 1 cut(s) 20
Bsp1286I GDGCHC 2 cut(s) 407, 661
Bsp143I GATC 5 cut(s) 220, 242, 409, 538, 601
BspACI CCGC 5 cut(s) 144, 561, 564, 585, 618
BspANI GGCC 4 cut(s) 193, 446, 675, 705
BspCNI CTCAG 5 cut(s) 118, 384, 419, 429, 601
BspDI ATCGAT 1 cut(s) 462
BspFNI CGCG 1 cut(s) 283
BspLI GGNNCC 1 cut(s) 704
BspPI GGATC 4 cut(s) 228, 250, 404, 596
BspTI CTTAAG 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 757
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 2 cut(s) 525, 659
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 4 cut(s) 172, 336, 447, 642
BssMI GATC 5 cut(s) 220, 242, 409, 538, 601
BssNI GRCGYC 2 cut(s) 180, 199
BssT1I CCWWGG 2 cut(s) 172, 447
Bst2UI CCWGG 2 cut(s) 643, 677
Bst4CI ACNGT 2 cut(s) 669, 727
BstACI GRCGYC 2 cut(s) 180, 199
BstAFI CTTAAG 1 cut(s) 34
BstAPI GCANNNNNTGC 1 cut(s) 497
BstC8I GCNNGC 1 cut(s) 715
BstDEI CTNAG 7 cut(s) 105, 371, 406, 416, 609, 662, 765
BstDSI CCRYGG 1 cut(s) 336
BstF5I GGATG 1 cut(s) 244
BstFNI CGCG 1 cut(s) 283
BstKTI GATC 5 cut(s) 223, 245, 412, 541, 604
BstMAI GTCTC 2 cut(s) 157, 757
BstMBI GATC 5 cut(s) 220, 242, 409, 538, 601
BstMWI GCNNNNNNNGC 4 cut(s) 66, 497, 585, 638
BstNI CCWGG 2 cut(s) 643, 677
BstSCI CCNGG 2 cut(s) 641, 675
BstUI CGCG 1 cut(s) 283
BstV2I GAAGAC 1 cut(s) 224
BstX2I RGATCY 2 cut(s) 409, 601
BstYI RGATCY 2 cut(s) 409, 601
Bsu15I ATCGAT 1 cut(s) 462
BsuRI GGCC 4 cut(s) 193, 446, 675, 705
BsuTUI ATCGAT 1 cut(s) 462
BtgI CCRYGG 1 cut(s) 336
BtgZI GCGATG 2 cut(s) 162, 582
BtsCI GGATG 1 cut(s) 244
BtsI GCAGTG 1 cut(s) 595
BtsIMutI CAGTG 4 cut(s) 379, 532, 595, 793
Cac8I GCNNGC 1 cut(s) 715
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 3 cut(s) 673, 684, 703
ClaI ATCGAT 1 cut(s) 462
CseI GACGC 3 cut(s) 13, 188, 289
CviAII CATG 1 cut(s) 747
DdeI CTNAG 7 cut(s) 105, 371, 406, 416, 609, 662, 765
DpnI GATC 5 cut(s) 222, 244, 411, 540, 603
DpnII GATC 5 cut(s) 220, 242, 409, 538, 601
DraIII CACNNNGTG 1 cut(s) 116
EaeI YGGCCR 1 cut(s) 444
Eco130I CCWWGG 2 cut(s) 172, 447
Eco24I GRGCYC 2 cut(s) 407, 661
Eco31I GGTCTC 1 cut(s) 757
Eco47I GGWCC 1 cut(s) 684
Eco88I CYCGRG 1 cut(s) 20
EcoO109I RGGNCCY 1 cut(s) 703
EcoRII CCWGG 2 cut(s) 641, 675
EcoT14I CCWWGG 2 cut(s) 172, 447
EcoT22I ATGCAT 1 cut(s) 748
EcoT38I GRGCYC 2 cut(s) 407, 661
ErhI CCWWGG 2 cut(s) 172, 447
FaeI CATG 1 cut(s) 750
FaiI YATR 8 cut(s) 212, 214, 440, 582, 639, 748, 782, 801
FaqI GGGAC 3 cut(s) 186, 375, 381
FatI CATG 1 cut(s) 746
Fnu4HI GCNGC 5 cut(s) 145, 562, 565, 586, 619
FokI GGATG 1 cut(s) 251
FriOI GRGCYC 2 cut(s) 407, 661
Fsp4HI GCNGC 5 cut(s) 145, 562, 565, 586, 619
FspBI CTAG 3 cut(s) 89, 633, 756
GluI GCNGC 5 cut(s) 145, 562, 565, 586, 619
GsaI CCCAGC 2 cut(s) 385, 763
GsuI CTGGAG 2 cut(s) 417, 698
HaeIII GGCC 4 cut(s) 193, 446, 675, 705
HapII CCGG 1 cut(s) 183
HgaI GACGC 3 cut(s) 13, 188, 289
Hin1I GRCGYC 2 cut(s) 180, 199
Hin1II CATG 1 cut(s) 750
HinfI GANTC 3 cut(s) 85, 250, 369
HpaII CCGG 1 cut(s) 183
HphI GGTGA 4 cut(s) 188, 365, 542, 548
Hpy166II GTNNAC 2 cut(s) 684, 730
Hpy188I TCNGA 5 cut(s) 220, 247, 255, 409, 419
Hpy188III TCNNGA 5 cut(s) 22, 82, 89, 236, 776
Hpy8I GTNNAC 2 cut(s) 684, 730
Hpy99I CGWCG 3 cut(s) 201, 310, 335
HpyAV CCTTC 4 cut(s) 133, 178, 314, 492
HpyCH4III ACNGT 2 cut(s) 669, 727
HpyCH4IV ACGT 2 cut(s) 199, 456
HpyCH4V TGCA 3 cut(s) 125, 500, 746
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 497, 585, 638
HpyF3I CTNAG 7 cut(s) 105, 371, 406, 416, 609, 662, 765
HpySE526I ACGT 2 cut(s) 199, 456
Hsp92I GRCGYC 2 cut(s) 180, 199
Hsp92II CATG 1 cut(s) 750
Kzo9I GATC 5 cut(s) 220, 242, 409, 538, 601
LweI GCATC 2 cut(s) 134, 733
MaeI CTAG 3 cut(s) 89, 633, 756
MaeII ACGT 2 cut(s) 199, 456
MaeIII GTNAC 1 cut(s) 176
MalI GATC 5 cut(s) 222, 244, 411, 540, 603
MboI GATC 5 cut(s) 220, 242, 409, 538, 601
MboII GAAGA 2 cut(s) 153, 224
MflI RGATCY 2 cut(s) 409, 601
MhlI GDGCHC 2 cut(s) 407, 661
MlsI TGGCCA 1 cut(s) 446
MluCI AATT 5 cut(s) 77, 474, 512, 794, 802
MluNI TGGCCA 1 cut(s) 446
MlyI GAGTC 2 cut(s) 79, 363
MmeI TCCRAC 1 cut(s) 534
MnlI CCTC 5 cut(s) 91, 204, 423, 558, 604
Mox20I TGGCCA 1 cut(s) 446
Mph1103I ATGCAT 1 cut(s) 748
MscI TGGCCA 1 cut(s) 446
MseI TTAA 4 cut(s) 35, 207, 771, 817
Msp20I TGGCCA 1 cut(s) 446
MspA1I CMGCKG 1 cut(s) 618
MspCI CTTAAG 1 cut(s) 34
MspI CCGG 1 cut(s) 183
MspR9I CCNGG 2 cut(s) 643, 677
MvaI CCWGG 2 cut(s) 643, 677
MvnI CGCG 1 cut(s) 283
MwoI GCNNNNNNNGC 4 cut(s) 66, 497, 585, 638
NdeII GATC 5 cut(s) 220, 242, 409, 538, 601
NlaIII CATG 1 cut(s) 750
NlaIV GGNNCC 1 cut(s) 704
NmuCI GTSAC 1 cut(s) 176
NsiI ATGCAT 1 cut(s) 748
PfeI GAWTC 1 cut(s) 250
PflMI CCANNNNNTGG 1 cut(s) 116
PkrI GCNGC 5 cut(s) 146, 563, 566, 587, 620
PleI GAGTC 2 cut(s) 79, 363
PpsI GAGTC 2 cut(s) 79, 363
Psp6I CCWGG 2 cut(s) 641, 675
PspFI CCCAGC 2 cut(s) 381, 759
PspGI CCWGG 2 cut(s) 641, 675
PspN4I GGNNCC 1 cut(s) 704
PspPI GGNCC 3 cut(s) 673, 684, 703
PsuI RGATCY 2 cut(s) 409, 601
SaqAI TTAA 4 cut(s) 35, 207, 771, 817
SatI GCNGC 5 cut(s) 145, 562, 565, 586, 619
Sau3AI GATC 5 cut(s) 220, 242, 409, 538, 601
Sau96I GGNCC 3 cut(s) 673, 684, 703
SchI GAGTC 2 cut(s) 79, 363
ScrFI CCNGG 2 cut(s) 643, 677
SduI GDGCHC 2 cut(s) 407, 661
SfaNI GCATC 2 cut(s) 134, 733
SgrAI CRCCGGYG 1 cut(s) 182
SinI GGWCC 1 cut(s) 684
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
Sse9I AATT 5 cut(s) 77, 474, 512, 794, 802
SsiI CCGC 5 cut(s) 144, 561, 564, 585, 618
SspMI CTAG 3 cut(s) 89, 633, 756
StyD4I CCNGG 2 cut(s) 641, 675
StyI CCWWGG 2 cut(s) 172, 447
TaaI ACNGT 2 cut(s) 669, 727
TaiI ACGT 2 cut(s) 202, 459
TaqI TCGA 3 cut(s) 196, 431, 462
TasI AATT 5 cut(s) 77, 474, 512, 794, 802
TauI GCSGC 5 cut(s) 147, 564, 567, 588, 621
TfiI GAWTC 1 cut(s) 250
Tru1I TTAA 4 cut(s) 35, 207, 771, 817
Tru9I TTAA 4 cut(s) 35, 207, 771, 817
TscAI CASTG 4 cut(s) 379, 532, 595, 793
TseFI GTSAC 1 cut(s) 176
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 1 cut(s) 788
TspRI CASTG 4 cut(s) 379, 532, 595, 793
Van91I CCANNNNNTGG 1 cut(s) 116
Vha464I CTTAAG 1 cut(s) 34
VpaK11BI GGWCC 1 cut(s) 684
XapI RAATTY 2 cut(s) 77, 794
XbaI TCTAGA 1 cut(s) 88
XspI CTAG 3 cut(s) 89, 633, 756
ZraI GACGTC 1 cut(s) 200
Zsp2I ATGCAT 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.