Rh5DG114300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000981
Physical Location & Seq
Forward (+)
10353995 .. 10354288
294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG114300.1

Sequence Viewer

Length: 294 bp
ATGATGGTGTGGCTCTGCTTATCAGAGAAGATTATCTCTGATGGTGACGCTGCCTGGTGGAGCGATGAGCTCTACATCGTCGGTGGTGCCGGCGATCATGGTGATGAGGTCAAGCGGCCATGGGTCAGATGCGGCATCGGCGTCTTCCAGATTGGGTTGTCTCCAACTGTGGGCGTAGGCGGTGGTGTCACAAGTGGCAGGTACAGACTCGGTGGTAGCTTTTGCGCTGGTGCCGATCCTGACGCTTGGGTATGTGGCGTCTCAATTGCTAGGGCGTCAAGGCTGAGTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

97

Amino Acids

10.12

Weight (kDa)

5.11

Isoelectric Point (pI)

16.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 189
AccB1I GGYRCC 2 cut(s) 86, 230
AciI CCGC 3 cut(s) 115, 132, 180
AclWI GGATC 1 cut(s) 230
AcoI YGGCCR 1 cut(s) 116
AcyI GRCGYC 3 cut(s) 141, 258, 275
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 1 cut(s) 170
AjnI CCWGG 1 cut(s) 53
AluBI AGCT 2 cut(s) 70, 219
AluI AGCT 2 cut(s) 70, 219
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 2 cut(s) 165, 265
AlwI GGATC 1 cut(s) 230
AoxI GGCC 1 cut(s) 116
ApeKI GCWGC 1 cut(s) 50
AspLEI GCGC 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 56, 113
BanI GGYRCC 2 cut(s) 86, 230
BanII GRGCYC 1 cut(s) 72
BbsI GAAGAC 1 cut(s) 136
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 1 cut(s) 37
BccI CCATC 1 cut(s) 35
BciT130I CCWGG 1 cut(s) 55
BcoDI GTCTC 2 cut(s) 165, 265
BfaI CTAG 1 cut(s) 270
BfuAI ACCTGC 1 cut(s) 189
BisI GCNGC 3 cut(s) 51, 116, 133
BlsI GCNGC 3 cut(s) 52, 117, 134
Bme1390I CCNGG 1 cut(s) 55
BmiI GGNNCC 2 cut(s) 88, 232
BmrFI CCNGG 1 cut(s) 55
BmsI GCATC 2 cut(s) 119, 144
BpiI GAAGAC 1 cut(s) 136
BsaHI GRCGYC 3 cut(s) 141, 258, 275
BsaJI CCNNGG 1 cut(s) 119
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse118I RCCGGY 1 cut(s) 89
BseBI CCWGG 1 cut(s) 55
BseDI CCNNGG 1 cut(s) 119
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMII CTCAG 1 cut(s) 275
BseXI GCAGC 1 cut(s) 37
BshFI GGCC 1 cut(s) 118
BshNI GGYRCC 2 cut(s) 86, 230
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 1 cut(s) 90
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 2 cut(s) 165, 265
BsmBI CGTCTC 1 cut(s) 265
BsnI GGCC 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 2 cut(s) 94, 235
Bsp19I CCATGG 1 cut(s) 119
BspACI CCGC 3 cut(s) 115, 132, 180
BspANI GGCC 1 cut(s) 118
BspCNI CTCAG 1 cut(s) 276
BspLI GGNNCC 2 cut(s) 88, 232
BspMI ACCTGC 1 cut(s) 189
BspPI GGATC 1 cut(s) 230
BspT107I GGYRCC 2 cut(s) 86, 230
BsrFI RCCGGY 1 cut(s) 89
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 2 cut(s) 94, 235
BssNI GRCGYC 3 cut(s) 141, 258, 275
BssT1I CCWWGG 1 cut(s) 119
Bst2UI CCWGG 1 cut(s) 55
Bst4CI ACNGT 1 cut(s) 169
BstACI GRCGYC 3 cut(s) 141, 258, 275
BstC8I GCNNGC 1 cut(s) 91
BstDEI CTNAG 1 cut(s) 284
BstDSI CCRYGG 1 cut(s) 119
BstHHI GCGC 1 cut(s) 227
BstKTI GATC 2 cut(s) 97, 238
BstMAI GTCTC 2 cut(s) 165, 265
BstMBI GATC 2 cut(s) 94, 235
BstMWI GCNNNNNNNGC 1 cut(s) 138
BstNI CCWGG 1 cut(s) 55
BstSCI CCNGG 1 cut(s) 53
BstV1I GCAGC 1 cut(s) 37
BstV2I GAAGAC 1 cut(s) 136
BsuRI GGCC 1 cut(s) 118
BtgI CCRYGG 1 cut(s) 119
BtgZI GCGATG 1 cut(s) 78
BveI ACCTGC 1 cut(s) 189
Cac8I GCNNGC 1 cut(s) 91
CfoI GCGC 1 cut(s) 227
Cfr10I RCCGGY 1 cut(s) 89
CseI GACGC 5 cut(s) 56, 130, 247, 251, 264
Csp6I GTAC 1 cut(s) 202
CviAII CATG 2 cut(s) 98, 120
CviJI RGCY 5 cut(s) 13, 70, 118, 219, 283
CviKI_1 RGCY 5 cut(s) 13, 70, 118, 219, 283
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 1 cut(s) 284
DpnI GATC 2 cut(s) 96, 237
DpnII GATC 2 cut(s) 94, 235
EaeI YGGCCR 1 cut(s) 116
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 72
Eco53kI GAGCTC 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 53
EcoT14I CCWWGG 1 cut(s) 119
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 119
Esp3I CGTCTC 1 cut(s) 265
FaeI CATG 2 cut(s) 101, 123
FaiI YATR 3 cut(s) 99, 121, 253
FatI CATG 2 cut(s) 97, 119
Fnu4HI GCNGC 3 cut(s) 51, 116, 133
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 3 cut(s) 51, 116, 133
FspBI CTAG 1 cut(s) 270
GlaI GCGC 1 cut(s) 226
GluI GCNGC 3 cut(s) 51, 116, 133
HaeIII GGCC 1 cut(s) 118
HapII CCGG 1 cut(s) 90
HgaI GACGC 5 cut(s) 56, 130, 247, 251, 264
HhaI GCGC 1 cut(s) 227
Hin1I GRCGYC 3 cut(s) 141, 258, 275
Hin1II CATG 2 cut(s) 101, 123
Hin6I GCGC 1 cut(s) 225
HinP1I GCGC 1 cut(s) 225
HinfI GANTC 1 cut(s) 207
HpaII CCGG 1 cut(s) 90
HphI GGTGA 2 cut(s) 56, 113
Hpy188I TCNGA 3 cut(s) 25, 40, 128
Hpy188III TCNNGA 2 cut(s) 148, 239
Hpy99I CGWCG 1 cut(s) 83
HpyCH4III ACNGT 1 cut(s) 169
HpyF10VI GCNNNNNNNGC 1 cut(s) 138
HpyF3I CTNAG 1 cut(s) 284
Hsp92I GRCGYC 3 cut(s) 141, 258, 275
Hsp92II CATG 2 cut(s) 101, 123
HspAI GCGC 1 cut(s) 225
KroI GCCGGC 1 cut(s) 89
KroNI GCCGGC 1 cut(s) 91
Kzo9I GATC 2 cut(s) 94, 235
LmnI GCTCC 1 cut(s) 60
LpnPI CCDG 7 cut(s) 40, 67, 103, 161, 184, 213, 252
Lsp1109I GCAGC 1 cut(s) 37
LweI GCATC 2 cut(s) 119, 144
MaeI CTAG 1 cut(s) 270
MaeIII GTNAC 2 cut(s) 44, 187
MalI GATC 2 cut(s) 96, 237
MboI GATC 2 cut(s) 94, 235
MboII GAAGA 2 cut(s) 40, 136
MfeI CAATTG 1 cut(s) 264
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 1 cut(s) 264
MlyI GAGTC 1 cut(s) 201
MmeI TCCRAC 1 cut(s) 188
MnlI CCTC 1 cut(s) 100
MroNI GCCGGC 1 cut(s) 89
MslI CAYNNNNRTG 1 cut(s) 102
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 1 cut(s) 55
MunI CAATTG 1 cut(s) 264
MvaI CCWGG 1 cut(s) 55
MwoI GCNNNNNNNGC 1 cut(s) 138
NaeI GCCGGC 1 cut(s) 91
NcoI CCATGG 1 cut(s) 119
NdeII GATC 2 cut(s) 94, 235
NgoMIV GCCGGC 1 cut(s) 89
NlaIII CATG 2 cut(s) 101, 123
NlaIV GGNNCC 2 cut(s) 88, 232
NmuCI GTSAC 2 cut(s) 44, 187
PdiI GCCGGC 1 cut(s) 91
PkrI GCNGC 3 cut(s) 52, 117, 134
PleI GAGTC 1 cut(s) 201
PpsI GAGTC 1 cut(s) 201
Psp124BI GAGCTC 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 53
PspGI CCWGG 1 cut(s) 53
PspN4I GGNNCC 2 cut(s) 88, 232
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
RseI CAYNNNNRTG 1 cut(s) 102
SacI GAGCTC 1 cut(s) 72
SatI GCNGC 3 cut(s) 51, 116, 133
Sau3AI GATC 2 cut(s) 94, 235
SchI GAGTC 1 cut(s) 201
ScrFI CCNGG 1 cut(s) 55
SduI GDGCHC 1 cut(s) 72
SetI ASST 4 cut(s) 72, 111, 203, 221
SfaNI GCATC 2 cut(s) 119, 144
SmiMI CAYNNNNRTG 1 cut(s) 102
Sse9I AATT 1 cut(s) 264
SsiI CCGC 3 cut(s) 115, 132, 180
SspMI CTAG 1 cut(s) 270
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 1 cut(s) 53
StyI CCWWGG 1 cut(s) 119
TaaI ACNGT 1 cut(s) 169
TasI AATT 1 cut(s) 264
TauI GCSGC 2 cut(s) 118, 135
TseFI GTSAC 2 cut(s) 44, 187
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 2 cut(s) 44, 187
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.