RLG00000017316

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000981
Physical Location & Seq
Reverse (-)
15188432 .. 15189787
1356 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017316

Sequence Viewer

Length: 420 bp
ATGCCATTTCCTCATTACTTGGTTCTGATCTGGGATGCTTATTCTCCTACGCGCGTCGGAGATGGCAGTTCCTTGACTGCGTTCTGGCGAAGGTGCCGGAGCGGTGCTATGCTGGGTCCGGAGGAGTTGGGCCAGAAGGGTGGTGACGCTCTTCTTTCTCCTACCCTTCTGGGTGGTAGCGGTGGATTGTTTCGTCGGTGCCCCTTTGCTTCAGATCTTGATCGTATTCGTCTGTTAGGTGTAACGCCTGGCTTTGGGTGGATCCAGAGACATGCTTTAATTGGTGATGTTGACGATGGAGTGGCTGGTCATGGTGGCGCTATTAATGGTAGAATTGGCGACGGCGGTAGCGGCGGCTTCTCCAGCTACGGTGACGGCGTTGGGGTGAAGGAGTGCACCATGCCATTCCAATTCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

140

Amino Acids

14.6

Weight (kDa)

6.25

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 93, 198
AccBSI CCGCTC 1 cut(s) 102
AccII CGCG 2 cut(s) 52, 54
AccIII TCCGGA 1 cut(s) 118
AciI CCGC 5 cut(s) 102, 180, 345, 351, 354
AclWI GGATC 2 cut(s) 256, 269
AcuI CTGAAG 1 cut(s) 195
AfiI CCNNNNNNNGG 1 cut(s) 254
AgsI TTSAA 1 cut(s) 415
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 1 cut(s) 366
AluI AGCT 1 cut(s) 366
Alw21I GWGCWC 1 cut(s) 398
Alw26I GTCTC 1 cut(s) 262
Alw44I GTGCAC 1 cut(s) 394
AlwI GGATC 2 cut(s) 256, 269
Aor13HI TCCGGA 1 cut(s) 118
AoxI GGCC 1 cut(s) 130
ApaLI GTGCAC 1 cut(s) 394
AseI ATTAAT 1 cut(s) 324
AspLEI GCGC 2 cut(s) 54, 320
AspS9I GGNCC 2 cut(s) 116, 130
AsuHPI GGTGA 4 cut(s) 155, 296, 383, 397
AvaII GGWCC 1 cut(s) 116
BaeGI GKGCMC 2 cut(s) 203, 398
BamHI GGATCC 1 cut(s) 261
BanI GGYRCC 2 cut(s) 93, 198
Bbv12I GWGCWC 1 cut(s) 398
BccI CCATC 2 cut(s) 56, 290
BceAI ACGGC 2 cut(s) 358, 391
BciT130I CCWGG 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 262
BfoI RGCGCY 1 cut(s) 321
BglII AGATCT 1 cut(s) 214
BisI GCNGC 2 cut(s) 352, 355
BlsI GCNGC 2 cut(s) 353, 356
Bme1390I CCNGG 1 cut(s) 249
Bme18I GGWCC 1 cut(s) 116
BmgT120I GGNCC 2 cut(s) 116, 130
BmiI GGNNCC 4 cut(s) 95, 117, 200, 263
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 1 cut(s) 25
BpmI CTGGAG 1 cut(s) 346
BsaBI GATNNNNATC 1 cut(s) 219
BsaWI WCCGGW 1 cut(s) 118
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse8I GATNNNNATC 1 cut(s) 219
BseAI TCCGGA 1 cut(s) 118
BseBI CCWGG 1 cut(s) 249
BseGI GGATG 1 cut(s) 40
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 1 cut(s) 254
BseRI GAGGAG 1 cut(s) 137
BseSI GKGCMC 2 cut(s) 203, 398
BseYI CCCAGC 1 cut(s) 112
Bsh1236I CGCG 2 cut(s) 52, 54
BshFI GGCC 1 cut(s) 132
BshNI GGYRCC 2 cut(s) 93, 198
BsiHKAI GWGCWC 1 cut(s) 398
BsiSI CCGG 2 cut(s) 97, 119
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 262
BsnI GGCC 1 cut(s) 132
Bsp1286I GDGCHC 2 cut(s) 203, 398
Bsp13I TCCGGA 1 cut(s) 118
Bsp143I GATC 4 cut(s) 27, 214, 220, 261
BspACI CCGC 5 cut(s) 102, 180, 345, 351, 354
BspANI GGCC 1 cut(s) 132
BspEI TCCGGA 1 cut(s) 118
BspFNI CGCG 2 cut(s) 52, 54
BspLI GGNNCC 4 cut(s) 95, 117, 200, 263
BspPI GGATC 2 cut(s) 256, 269
BspQI GCTCTTC 1 cut(s) 156
BspT107I GGYRCC 2 cut(s) 93, 198
BsrBI CCGCTC 1 cut(s) 102
BssMI GATC 4 cut(s) 27, 214, 220, 261
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 1 cut(s) 371
Bst6I CTCTTC 1 cut(s) 156
BstF5I GGATG 1 cut(s) 40
BstFNI CGCG 2 cut(s) 52, 54
BstH2I RGCGCY 1 cut(s) 321
BstHHI GCGC 2 cut(s) 54, 320
BstKTI GATC 4 cut(s) 30, 217, 223, 264
BstMAI GTCTC 1 cut(s) 262
BstMBI GATC 4 cut(s) 27, 214, 220, 261
BstMWI GCNNNNNNNGC 2 cut(s) 351, 363
BstNI CCWGG 1 cut(s) 249
BstNSI RCATGY 1 cut(s) 275
BstSCI CCNGG 1 cut(s) 247
BstSLI GKGCMC 2 cut(s) 203, 398
BstUI CGCG 2 cut(s) 52, 54
BstX2I RGATCY 2 cut(s) 214, 261
BstXI CCANNNNNNTGG 1 cut(s) 140
BstYI RGATCY 2 cut(s) 214, 261
BsuRI GGCC 1 cut(s) 132
BtsCI GGATG 1 cut(s) 40
CfoI GCGC 2 cut(s) 54, 320
Cfr13I GGNCC 2 cut(s) 116, 130
CseI GACGC 2 cut(s) 43, 155
CviAII CATG 3 cut(s) 272, 311, 400
CviJI RGCY 5 cut(s) 132, 252, 305, 357, 366
CviKI_1 RGCY 5 cut(s) 132, 252, 305, 357, 366
DpnI GATC 4 cut(s) 29, 216, 222, 263
DpnII GATC 4 cut(s) 27, 214, 220, 261
Eam1104I CTCTTC 1 cut(s) 156
EarI CTCTTC 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 116
Eco57I CTGAAG 1 cut(s) 195
EcoRII CCWGG 1 cut(s) 247
FaeI CATG 3 cut(s) 275, 314, 403
FaiI YATR 4 cut(s) 110, 273, 312, 401
FatI CATG 3 cut(s) 271, 310, 399
Fnu4HI GCNGC 2 cut(s) 352, 355
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 2 cut(s) 352, 355
GlaI GCGC 2 cut(s) 53, 319
GluI GCNGC 2 cut(s) 352, 355
GsaI CCCAGC 1 cut(s) 116
GsuI CTGGAG 1 cut(s) 346
HaeII RGCGCY 1 cut(s) 321
HaeIII GGCC 1 cut(s) 132
HapII CCGG 2 cut(s) 97, 119
HgaI GACGC 2 cut(s) 43, 155
HhaI GCGC 2 cut(s) 54, 320
Hin1II CATG 3 cut(s) 275, 314, 403
Hin6I GCGC 2 cut(s) 52, 318
HinP1I GCGC 2 cut(s) 52, 318
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HpaII CCGG 2 cut(s) 97, 119
HphI GGTGA 4 cut(s) 155, 296, 383, 397
Hpy166II GTNNAC 2 cut(s) 292, 396
Hpy188I TCNGA 3 cut(s) 27, 59, 214
Hpy188III TCNNGA 3 cut(s) 119, 218, 265
Hpy8I GTNNAC 2 cut(s) 292, 396
Hpy99I CGWCG 3 cut(s) 59, 198, 344
HpyAV CCTTC 4 cut(s) 84, 130, 176, 382
HpyCH4III ACNGT 1 cut(s) 371
HpyCH4V TGCA 1 cut(s) 396
HpyF10VI GCNNNNNNNGC 2 cut(s) 351, 363
Hsp92II CATG 3 cut(s) 275, 314, 403
HspAI GCGC 2 cut(s) 52, 318
Kpn2I TCCGGA 1 cut(s) 118
Kzo9I GATC 4 cut(s) 27, 214, 220, 261
LguI GCTCTTC 1 cut(s) 156
LmnI GCTCC 1 cut(s) 99
LweI GCATC 1 cut(s) 25
MaeIII GTNAC 3 cut(s) 143, 241, 371
MalI GATC 4 cut(s) 29, 216, 222, 263
MbiI CCGCTC 1 cut(s) 102
MboI GATC 4 cut(s) 27, 214, 220, 261
MboII GAAGA 1 cut(s) 143
MflI RGATCY 2 cut(s) 214, 261
MhlI GDGCHC 2 cut(s) 203, 398
MluCI AATT 4 cut(s) 279, 333, 410, 415
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 2 cut(s) 21, 115
MroI TCCGGA 1 cut(s) 118
MseI TTAA 2 cut(s) 278, 324
MspI CCGG 2 cut(s) 97, 119
MspR9I CCNGG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 249
MvnI CGCG 2 cut(s) 52, 54
MwoI GCNNNNNNNGC 2 cut(s) 351, 363
NdeII GATC 4 cut(s) 27, 214, 220, 261
NlaIII CATG 3 cut(s) 275, 314, 403
NlaIV GGNNCC 4 cut(s) 95, 117, 200, 263
NmuCI GTSAC 2 cut(s) 143, 371
NspI RCATGY 1 cut(s) 275
PciSI GCTCTTC 1 cut(s) 156
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PkrI GCNGC 2 cut(s) 353, 356
PshBI ATTAAT 1 cut(s) 324
Psp6I CCWGG 1 cut(s) 247
PspFI CCCAGC 1 cut(s) 112
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 4 cut(s) 95, 117, 200, 263
PspPI GGNCC 2 cut(s) 116, 130
PsuI RGATCY 2 cut(s) 214, 261
SapI GCTCTTC 1 cut(s) 156
SaqAI TTAA 2 cut(s) 278, 324
SatI GCNGC 2 cut(s) 352, 355
Sau3AI GATC 4 cut(s) 27, 214, 220, 261
Sau96I GGNCC 2 cut(s) 116, 130
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 2 cut(s) 203, 398
SetI ASST 3 cut(s) 95, 241, 368
SfaNI GCATC 1 cut(s) 25
SinI GGWCC 1 cut(s) 116
Sse9I AATT 4 cut(s) 279, 333, 410, 415
SsiI CCGC 5 cut(s) 102, 180, 345, 351, 354
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 1 cut(s) 371
TasI AATT 4 cut(s) 279, 333, 410, 415
TauI GCSGC 2 cut(s) 354, 357
Tru1I TTAA 2 cut(s) 278, 324
Tru9I TTAA 2 cut(s) 278, 324
TseFI GTSAC 2 cut(s) 143, 371
Tsp45I GTSAC 2 cut(s) 143, 371
VneI GTGCAC 1 cut(s) 394
VpaK11BI GGWCC 1 cut(s) 116
VspI ATTAAT 1 cut(s) 324
XceI RCATGY 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.