Rmu_sc0000195.1_g000037

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000195.1
OG0001043
Physical Location & Seq
Forward (+)
118628 .. 125113
6486 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1503 bp
atgactcgaagcaagcttgatcggggagaacgtatcaatcgcatcgaaactcgacacctaggaggtaagagttgcgacggggagggagatgtttatcagcgtgtcacttgcatcgaaacctcttgccaagacggtgtgggttttgacggggagggagacgttggcccttttcatcacagtgctctattgtgtgagtcggacgaaaaggtggaactggttagttttgactatgaaggttcaaatactatcttcaatttttcagttgataaaagtttggaaaaggaaaataaagatggggttgtggattttagtttgccaccaatttataattgtttttgtgaggcttgcagtggcaattgtgatgatcagatttgtgatggaaattgtttggaggttgaaggtaatattgatgaacatggtagcaatactattgataaagggtctgagctagcagtaggaaatattactatgcacatgcaaggaattgatccaattatacaagtcgatgaagctactataccaccaattgaagtggaagacctatacccatatataggtattgacaaaattgatgtgggtgagaggattgatgatatgaactcaaaccatggtcatattggtgatgacaacgtgggtttcagtattggacttccaagaactaggcatgagccggattcaatcttggatgagagtactacaagtcttctctcttcccagcctcctctgccgtcaaaactcaaaacacatcttcccatcgatcgattccgtcgctcctccgccacacgccccaaacccaaagcattcccggaccccagcttcttcaacctcctttcgtacttggttaaaatttgtgcgtggaaggctgcttcaaatatcctacctcctttgagtctgcttagtgaacgtggtatcgatcttgatacccaatgcccattctgcaaggaggaaattgaatcccctctacatgcactacgtgaatgtatcaatgccaagtctttcttgcagttggctaacctccctagctcagattctacttcaagtgttgttgattggttagagacttcctattctcagtctcctactacctttgttttcctgttgatggtgatttcggctacgtggagaaaccgcaatgacaaagtctggatcaatgctcctatggcgtcggaagttgttcccatgactgtagttaaacttgatgctgccttttgcaatgtgaatgggattgcagggtttggaggtattttccgagactttgaaggtacttgtctaggggacttttgccatacagttagggtcacaagttcagcaaggcatgctgatcgaattgagctcctacagtcagctgcttcagctcaggtttgctttgtgaggcgccaggccaactcggtggcgcataggctagctcaagaagccaagctggcttcgttttctttgaacttttttcatgtacctcctccaagtgtggtggagattataaaccatgattgtaatggtcattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PfkB TB2_DP1_HVA22

Protein Analysis

500

Amino Acids

55.06

Weight (kDa)

4.87

Isoelectric Point (pI)

43.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 327, 1478
AccB1I GGYRCC 1 cut(s) 1374
AciI CCGC 2 cut(s) 777, 1129
AclWI GGATC 2 cut(s) 482, 1154
AcsI RAATTY 1 cut(s) 846
AcuI CTGAAG 1 cut(s) 1335
AcyI GRCGYC 2 cut(s) 1163, 1375
AdeI CACNNNGTG 1 cut(s) 974
AfaI GTAC 4 cut(s) 694, 836, 1264, 1452
AfiI CCNNNNNNNGG 2 cut(s) 554, 1102
AjnI CCWGG 1 cut(s) 1377
Alw21I GWGCWC 2 cut(s) 184, 1335
Alw26I GTCTC 4 cut(s) 150, 1052, 1080, 1245
AlwI GGATC 2 cut(s) 482, 1154
AoxI GGCC 2 cut(s) 163, 1380
ApeKI GCWGC 3 cut(s) 863, 1202, 1346
ApoI RAATTY 1 cut(s) 846
Asp700I GAANNNNTTC 1 cut(s) 1173
AspA2I CCTAGG 1 cut(s) 58
AspLEI GCGC 2 cut(s) 1377, 1396
AspS9I GGNCC 2 cut(s) 164, 808
AsuC2I CCSGG 1 cut(s) 806
AsuHPI GGTGA 3 cut(s) 590, 632, 1117
AsuNHI GCTAGC 2 cut(s) 448, 1402
AvaII GGWCC 1 cut(s) 808
AvrII CCTAGG 1 cut(s) 58
BanI GGYRCC 1 cut(s) 1374
BanII GRGCYC 1 cut(s) 1335
BarI GAAGNNNNNNTAC 2 cut(s) 501, 533
BbsI GAAGAC 2 cut(s) 543, 695
Bbv12I GWGCWC 2 cut(s) 184, 1335
BbvI GCAGC 3 cut(s) 850, 1189, 1333
BccI CCATC 4 cut(s) 287, 371, 761, 1096
BceAI ACGGC 1 cut(s) 712
BcgI CGANNNNNNTGC 2 cut(s) 1304, 1338
BciT130I CCWGG 1 cut(s) 1379
BclI TGATCA 1 cut(s) 364
BcnI CCSGG 1 cut(s) 806
BcoDI GTCTC 4 cut(s) 150, 1052, 1080, 1245
BfaI CTAG 6 cut(s) 59, 449, 660, 1020, 1271, 1403
BfmI CTRYAG 2 cut(s) 1185, 1337
BfoI RGCGCY 1 cut(s) 1378
BglI GCCNNNNNGGC 1 cut(s) 1421
BisI GCNGC 3 cut(s) 864, 1203, 1347
BlnI CCTAGG 1 cut(s) 58
BlsI GCNGC 3 cut(s) 865, 1204, 1348
BmcAI AGTACT 1 cut(s) 694
Bme1390I CCNGG 2 cut(s) 806, 1379
Bme18I GGWCC 1 cut(s) 808
BmgT120I GGNCC 2 cut(s) 164, 808
BmiI GGNNCC 2 cut(s) 810, 1376
BmrFI CCNGG 2 cut(s) 806, 1379
BmsI GCATC 3 cut(s) 51, 120, 1189
BmtI GCTAGC 2 cut(s) 452, 1406
BpiI GAAGAC 2 cut(s) 543, 695
Bpu10I CCTNAGC 1 cut(s) 1356
BpuEI CTTGAG 1 cut(s) 1392
BpuMI CCSGG 1 cut(s) 806
Bsa29I ATCGAT 3 cut(s) 756, 760, 912
BsaAI YACGTR 2 cut(s) 974, 1119
BsaBI GATNNNNATC 1 cut(s) 93
BsaHI GRCGYC 2 cut(s) 1163, 1375
BsaJI CCNNGG 2 cut(s) 58, 607
Bsc4I CCNNNNNNNGG 2 cut(s) 554, 1102
Bse1I ACTGG 1 cut(s) 219
Bse3DI GCAATG 2 cut(s) 1138, 1219
Bse8I GATNNNNATC 1 cut(s) 93
BseBI CCWGG 1 cut(s) 1379
BseCI ATCGAT 3 cut(s) 756, 760, 912
BseDI CCNNGG 2 cut(s) 58, 607
BseGI GGATG 1 cut(s) 691
BseJI GATNNNNATC 1 cut(s) 93
BseLI CCNNNNNNNGG 2 cut(s) 554, 1102
BseMI GCAATG 2 cut(s) 1138, 1219
BseMII CTCAG 4 cut(s) 435, 1038, 1085, 1370
BseNI ACTGG 1 cut(s) 219
BseRI GAGGAG 3 cut(s) 711, 763, 1446
BseXI GCAGC 3 cut(s) 850, 1189, 1333
BseYI CCCAGC 2 cut(s) 714, 812
Bsh1285I CGRYCG 1 cut(s) 760
BshFI GGCC 2 cut(s) 165, 1382
BshNI GGYRCC 1 cut(s) 1374
BshVI ATCGAT 3 cut(s) 756, 760, 912
BsiEI CGRYCG 1 cut(s) 760
BsiHKAI GWGCWC 2 cut(s) 184, 1335
BsiSI CCGG 2 cut(s) 671, 806
BslFI GGGAC 1 cut(s) 1289
BslI CCNNNNNNNGG 2 cut(s) 554, 1102
BsmAI GTCTC 4 cut(s) 150, 1052, 1080, 1245
BsmBI CGTCTC 1 cut(s) 150
BsmFI GGGAC 1 cut(s) 1289
BsmI GAATGC 1 cut(s) 800
BsnI GGCC 2 cut(s) 165, 1382
Bsp1286I GDGCHC 2 cut(s) 184, 1335
Bsp143I GATC 7 cut(s) 19, 364, 487, 757, 913, 1146, 1321
Bsp19I CCATGG 1 cut(s) 607
BspACI CCGC 2 cut(s) 777, 1129
BspANI GGCC 2 cut(s) 165, 1382
BspCNI CTCAG 4 cut(s) 436, 1037, 1084, 1369
BspDI ATCGAT 3 cut(s) 756, 760, 912
BspLI GGNNCC 2 cut(s) 810, 1376
BspOI GCTAGC 2 cut(s) 452, 1406
BspPI GGATC 2 cut(s) 482, 1154
BspT107I GGYRCC 1 cut(s) 1374
BsrDI GCAATG 2 cut(s) 1138, 1219
BsrI ACTGG 1 cut(s) 219
BssECI CCNNGG 2 cut(s) 58, 607
BssMI GATC 7 cut(s) 19, 364, 487, 757, 913, 1146, 1321
BssNI GRCGYC 2 cut(s) 1163, 1375
BssT1I CCWWGG 2 cut(s) 58, 607
Bst2UI CCWGG 1 cut(s) 1379
Bst4CI ACNGT 5 cut(s) 134, 179, 1186, 1291, 1341
Bst6I CTCTTC 1 cut(s) 715
BstACI GRCGYC 2 cut(s) 1163, 1375
BstAPI GCANNNNNTGC 1 cut(s) 1316
BstBAI YACGTR 2 cut(s) 974, 1119
BstC8I GCNNGC 6 cut(s) 14, 346, 450, 1317, 1404, 1422
BstDEI CTNAG 5 cut(s) 444, 896, 1024, 1071, 1356
BstDSI CCRYGG 1 cut(s) 607
BstF5I GGATG 1 cut(s) 691
BstH2I RGCGCY 1 cut(s) 1378
BstHHI GCGC 2 cut(s) 1377, 1396
BstKTI GATC 7 cut(s) 22, 367, 490, 760, 916, 1149, 1324
BstMAI GTCTC 4 cut(s) 150, 1052, 1080, 1245
BstMBI GATC 7 cut(s) 19, 364, 487, 757, 913, 1146, 1321
BstMCI CGRYCG 1 cut(s) 760
BstMWI GCNNNNNNNGC 8 cut(s) 724, 860, 936, 1160, 1316, 1352, 1412, 1421
BstNI CCWGG 1 cut(s) 1379
BstNSI RCATGY 3 cut(s) 478, 968, 1319
BstSCI CCNGG 2 cut(s) 804, 1377
BstSFI CTRYAG 2 cut(s) 1185, 1337
BstV1I GCAGC 3 cut(s) 850, 1189, 1333
BstV2I GAAGAC 2 cut(s) 543, 695
BstXI CCANNNNNNTGG 1 cut(s) 1390
Bsu15I ATCGAT 3 cut(s) 756, 760, 912
BsuRI GGCC 2 cut(s) 165, 1382
BsuTUI ATCGAT 3 cut(s) 756, 760, 912
BtgI CCRYGG 1 cut(s) 607
BtsCI GGATG 1 cut(s) 691
BtsI GCAGTG 1 cut(s) 355
BtsIMutI CAGTG 2 cut(s) 184, 355
Cac8I GCNNGC 6 cut(s) 14, 346, 450, 1317, 1404, 1422
CfoI GCGC 2 cut(s) 1377, 1396
Cfr13I GGNCC 2 cut(s) 164, 808
ClaI ATCGAT 3 cut(s) 756, 760, 912
CseI GACGC 1 cut(s) 1152
Csp6I GTAC 4 cut(s) 693, 835, 1263, 1451
CspCI CAANNNNNGTGG 4 cut(s) 513, 548, 1449, 1484
CviAII CATG 9 cut(s) 416, 475, 608, 665, 965, 1180, 1316, 1448, 1484
CviQI GTAC 4 cut(s) 693, 835, 1263, 1451
DdeI CTNAG 5 cut(s) 444, 896, 1024, 1071, 1356
DinI GGCGCC 1 cut(s) 1376
DpnI GATC 7 cut(s) 21, 366, 489, 759, 915, 1148, 1323
DpnII GATC 7 cut(s) 19, 364, 487, 757, 913, 1146, 1321
DraIII CACNNNGTG 1 cut(s) 974
Eam1104I CTCTTC 1 cut(s) 715
EarI CTCTTC 1 cut(s) 715
EciI GGCGGA 1 cut(s) 766
Ecl136II GAGCTC 1 cut(s) 1333
Eco130I CCWWGG 2 cut(s) 58, 607
Eco24I GRGCYC 1 cut(s) 1335
Eco47I GGWCC 1 cut(s) 808
Eco53kI GAGCTC 1 cut(s) 1333
Eco57I CTGAAG 1 cut(s) 1335
EcoICRI GAGCTC 1 cut(s) 1333
EcoRII CCWGG 1 cut(s) 1377
EcoT14I CCWWGG 2 cut(s) 58, 607
EcoT38I GRGCYC 1 cut(s) 1335
EgeI GGCGCC 1 cut(s) 1376
EheI GGCGCC 1 cut(s) 1376
ErhI CCWWGG 2 cut(s) 58, 607
Esp3I CGTCTC 1 cut(s) 150
FaeI CATG 9 cut(s) 419, 478, 611, 668, 968, 1183, 1319, 1451, 1487
FalI AAGNNNNNCTT 2 cut(s) 983, 1015
FaqI GGGAC 1 cut(s) 1289
FatI CATG 9 cut(s) 415, 474, 607, 664, 964, 1179, 1315, 1447, 1483
FbaI TGATCA 1 cut(s) 364
Fnu4HI GCNGC 3 cut(s) 864, 1203, 1347
FokI GGATG 1 cut(s) 698
FriOI GRGCYC 1 cut(s) 1335
Fsp4HI GCNGC 3 cut(s) 864, 1203, 1347
FspBI CTAG 6 cut(s) 59, 449, 660, 1020, 1271, 1403
GlaI GCGC 2 cut(s) 1376, 1395
GluI GCNGC 3 cut(s) 864, 1203, 1347
GsaI CCCAGC 2 cut(s) 718, 816
HaeII RGCGCY 1 cut(s) 1378
HaeIII GGCC 2 cut(s) 165, 1382
HapII CCGG 2 cut(s) 671, 806
HgaI GACGC 1 cut(s) 1152
HhaI GCGC 2 cut(s) 1377, 1396
Hin1I GRCGYC 2 cut(s) 1163, 1375
Hin1II CATG 9 cut(s) 419, 478, 611, 668, 968, 1183, 1319, 1451, 1487
Hin6I GCGC 2 cut(s) 1375, 1394
HinP1I GCGC 2 cut(s) 1375, 1394
HindIII AAGCTT 1 cut(s) 14
HinfI GANTC 7 cut(s) 4, 194, 674, 762, 889, 953, 1028
HpaII CCGG 2 cut(s) 671, 806
HphI GGTGA 3 cut(s) 590, 632, 1117
Hpy166II GTNNAC 1 cut(s) 902
Hpy188I TCNGA 6 cut(s) 199, 369, 445, 1027, 1168, 1250
Hpy188III TCNNGA 3 cut(s) 917, 1144, 1409
Hpy8I GTNNAC 1 cut(s) 902
Hpy99I CGWCG 3 cut(s) 80, 771, 1168
HpyAV CCTTC 4 cut(s) 227, 392, 853, 1253
HpyCH4III ACNGT 5 cut(s) 134, 179, 1186, 1291, 1341
HpyCH4IV ACGT 6 cut(s) 31, 159, 630, 904, 973, 1118
HpyCH4V TGCA 9 cut(s) 111, 348, 472, 478, 939, 968, 1003, 1212, 1229
HpyF10VI GCNNNNNNNGC 8 cut(s) 724, 860, 936, 1160, 1316, 1352, 1412, 1421
HpyF3I CTNAG 5 cut(s) 444, 896, 1024, 1071, 1356
HpySE526I ACGT 6 cut(s) 31, 159, 630, 904, 973, 1118
Hsp92I GRCGYC 2 cut(s) 1163, 1375
Hsp92II CATG 9 cut(s) 419, 478, 611, 668, 968, 1183, 1319, 1451, 1487
HspAI GCGC 2 cut(s) 1375, 1394
KasI GGCGCC 1 cut(s) 1374
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 7 cut(s) 19, 364, 487, 757, 913, 1146, 1321
LmnI GCTCC 3 cut(s) 776, 1159, 1338
Lsp1109I GCAGC 3 cut(s) 850, 1189, 1333
LweI GCATC 3 cut(s) 51, 120, 1189
MaeI CTAG 6 cut(s) 59, 449, 660, 1020, 1271, 1403
MaeII ACGT 6 cut(s) 31, 159, 630, 904, 973, 1118
MaeIII GTNAC 2 cut(s) 103, 1297
MalI GATC 7 cut(s) 21, 366, 489, 759, 915, 1148, 1323
MboI GATC 7 cut(s) 19, 364, 487, 757, 913, 1146, 1321
MboII GAAGA 6 cut(s) 241, 548, 695, 702, 740, 811
MfeI CAATTG 2 cut(s) 355, 525
MhlI GDGCHC 2 cut(s) 184, 1335
Mly113I GGCGCC 1 cut(s) 1375
MlyI GAGTC 2 cut(s) 203, 898
MmeI TCCRAC 2 cut(s) 177, 1146
MroXI GAANNNNTTC 1 cut(s) 1173
MseI TTAA 3 cut(s) 843, 1191, 1501
MslI CAYNNNNRTG 2 cut(s) 177, 618
MspA1I CMGCKG 1 cut(s) 1346
MspI CCGG 2 cut(s) 671, 806
MspR9I CCNGG 2 cut(s) 806, 1379
MunI CAATTG 2 cut(s) 355, 525
Mva1269I GAATGC 1 cut(s) 800
MvaI CCWGG 1 cut(s) 1379
MwoI GCNNNNNNNGC 8 cut(s) 724, 860, 936, 1160, 1316, 1352, 1412, 1421
NarI GGCGCC 1 cut(s) 1375
NciI CCSGG 1 cut(s) 806
NcoI CCATGG 1 cut(s) 607
NdeII GATC 7 cut(s) 19, 364, 487, 757, 913, 1146, 1321
NheI GCTAGC 2 cut(s) 448, 1402
NlaIII CATG 9 cut(s) 419, 478, 611, 668, 968, 1183, 1319, 1451, 1487
NlaIV GGNNCC 2 cut(s) 810, 1376
NmuCI GTSAC 2 cut(s) 103, 1297
NspI RCATGY 3 cut(s) 478, 968, 1319
PaeI GCATGC 1 cut(s) 1319
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PctI GAATGC 1 cut(s) 800
PdmI GAANNNNTTC 1 cut(s) 1173
PfeI GAWTC 4 cut(s) 674, 762, 953, 1028
PflFI GACNNNGTC 1 cut(s) 1139
PfoI TCCNGGA 1 cut(s) 804
PkrI GCNGC 3 cut(s) 865, 1204, 1348
Ple19I CGATCG 1 cut(s) 760
PleI GAGTC 2 cut(s) 202, 897
PluTI GGCGCC 1 cut(s) 1378
PpsI GAGTC 2 cut(s) 202, 897
Ppu21I YACGTR 2 cut(s) 974, 1119
PsiI TTATAA 2 cut(s) 327, 1478
Psp124BI GAGCTC 1 cut(s) 1335
Psp6I CCWGG 1 cut(s) 1377
PspFI CCCAGC 2 cut(s) 714, 812
PspGI CCWGG 1 cut(s) 1377
PspN4I GGNNCC 2 cut(s) 810, 1376
PspPI GGNCC 2 cut(s) 164, 808
PsyI GACNNNGTC 1 cut(s) 1139
PvuI CGATCG 1 cut(s) 760
PvuII CAGCTG 1 cut(s) 1346
RsaI GTAC 4 cut(s) 694, 836, 1264, 1452
RsaNI GTAC 4 cut(s) 693, 835, 1263, 1451
RseI CAYNNNNRTG 2 cut(s) 177, 618
SacI GAGCTC 1 cut(s) 1335
SaqAI TTAA 3 cut(s) 843, 1191, 1501
SatI GCNGC 3 cut(s) 864, 1203, 1347
Sau3AI GATC 7 cut(s) 19, 364, 487, 757, 913, 1146, 1321
Sau96I GGNCC 2 cut(s) 164, 808
ScaI AGTACT 1 cut(s) 694
SchI GAGTC 2 cut(s) 203, 898
ScrFI CCNGG 2 cut(s) 806, 1379
SduI GDGCHC 2 cut(s) 184, 1335
SfaNI GCATC 3 cut(s) 51, 120, 1189
SfcI CTRYAG 2 cut(s) 1185, 1337
SfoI GGCGCC 1 cut(s) 1376
SinI GGWCC 1 cut(s) 808
SmiMI CAYNNNNRTG 2 cut(s) 177, 618
SmlI CTYRAG 1 cut(s) 1407
SmoI CTYRAG 1 cut(s) 1407
SphI GCATGC 1 cut(s) 1319
SsiI CCGC 2 cut(s) 777, 1129
SspDI GGCGCC 1 cut(s) 1374
SspI AATATT 2 cut(s) 406, 463
SspMI CTAG 6 cut(s) 59, 449, 660, 1020, 1271, 1403
SstI GAGCTC 1 cut(s) 1335
StyD4I CCNGG 2 cut(s) 804, 1377
StyI CCWWGG 2 cut(s) 58, 607
TaaI ACNGT 5 cut(s) 134, 179, 1186, 1291, 1341
TaiI ACGT 6 cut(s) 34, 162, 633, 907, 976, 1121
TaqI TCGA 9 cut(s) 7, 45, 52, 114, 504, 756, 760, 912, 1324
TatI WGTACW 1 cut(s) 692
TfiI GAWTC 4 cut(s) 674, 762, 953, 1028
Tru1I TTAA 3 cut(s) 843, 1191, 1501
Tru9I TTAA 3 cut(s) 843, 1191, 1501
TscAI CASTG 2 cut(s) 184, 355
TseFI GTSAC 2 cut(s) 103, 1297
TseI GCWGC 3 cut(s) 863, 1202, 1346
Tsp45I GTSAC 2 cut(s) 103, 1297
TspDTI ATGAA 6 cut(s) 161, 246, 426, 522, 611, 1436
TspGWI ACGGA 1 cut(s) 755
TspRI CASTG 2 cut(s) 184, 355
Tth111I GACNNNGTC 1 cut(s) 1139
VpaK11BI GGWCC 1 cut(s) 808
XapI RAATTY 1 cut(s) 846
XceI RCATGY 3 cut(s) 478, 968, 1319
XcmI CCANNNNNNNNNTGG 2 cut(s) 614, 1490
XmaJI CCTAGG 1 cut(s) 58
XmnI GAANNNNTTC 1 cut(s) 1173
XspI CTAG 6 cut(s) 59, 449, 660, 1020, 1271, 1403
ZrmI AGTACT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.