Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1553 |
| Acc36I | ACCTGC | 1 cut(s) | 1706 |
| AccI | GTMKAC | 1 cut(s) | 243 |
| AccII | CGCG | 2 cut(s) | 87, 658 |
| AciI | CCGC | 4 cut(s) | 23, 53, 87, 1405 |
| AclWI | GGATC | 8 cut(s) | 131, 144, 212, 356, 580, 756, 1629, 1716 |
| AcoI | YGGCCR | 4 cut(s) | 88, 261, 502, 1132 |
| AcsI | RAATTY | 1 cut(s) | 431 |
| AcuI | CTGAAG | 2 cut(s) | 1103, 1974 |
| AdeI | CACNNNGTG | 2 cut(s) | 359, 1006 |
| AfaI | GTAC | 3 cut(s) | 229, 710, 1318 |
| AfeI | AGCGCT | 2 cut(s) | 424, 1955 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 533, 1276, 1763, 1796, 1835, 1841 |
| AflIII | ACRYGT | 3 cut(s) | 443, 1084, 1659 |
| AjnI | CCWGG | 3 cut(s) | 364, 1834, 1911 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 143, 175 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 360, 392 |
| AluBI | AGCT | 8 cut(s) | 17, 578, 770, 1210, 1291, 1333, 1445, 1597 |
| AluI | AGCT | 8 cut(s) | 17, 578, 770, 1210, 1291, 1333, 1445, 1597 |
| Alw21I | GWGCWC | 3 cut(s) | 19, 1293, 2025 |
| Alw26I | GTCTC | 3 cut(s) | 293, 941, 1977 |
| AlwI | GGATC | 8 cut(s) | 131, 144, 212, 356, 580, 756, 1629, 1716 |
| Ama87I | CYCGRG | 1 cut(s) | 1292 |
| Aor51HI | AGCGCT | 2 cut(s) | 424, 1955 |
| ApeKI | GCWGC | 2 cut(s) | 1196, 1806 |
| ApoI | RAATTY | 1 cut(s) | 431 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 197, 229, 865, 897 |
| AspLEI | GCGC | 5 cut(s) | 87, 425, 1363, 1554, 1956 |
| AspS9I | GGNCC | 4 cut(s) | 180, 523, 1746, 1826 |
| AsuHPI | GGTGA | 2 cut(s) | 37, 67 |
| AsuNHI | GCTAGC | 1 cut(s) | 2033 |
| AvaI | CYCGRG | 1 cut(s) | 1292 |
| AvaII | GGWCC | 3 cut(s) | 180, 523, 1826 |
| AxyI | CCTNAGG | 1 cut(s) | 554 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 1071, 1071, 1104, 1104 |
| BalI | TGGCCA | 1 cut(s) | 1134 |
| BamHI | GGATCC | 1 cut(s) | 136 |
| BanII | GRGCYC | 2 cut(s) | 19, 1293 |
| BbsI | GAAGAC | 1 cut(s) | 1109 |
| Bbv12I | GWGCWC | 3 cut(s) | 19, 1293, 2025 |
| BbvI | GCAGC | 2 cut(s) | 1183, 1818 |
| BccI | CCATC | 6 cut(s) | 800, 1367, 1456, 1529, 1669, 1736 |
| BceAI | ACGGC | 3 cut(s) | 52, 75, 248 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 636, 670 |
| BciT130I | CCWGG | 3 cut(s) | 366, 1836, 1913 |
| BciVI | GTATCC | 3 cut(s) | 162, 985, 1072 |
| BcoDI | GTCTC | 3 cut(s) | 293, 941, 1977 |
| BfaI | CTAG | 3 cut(s) | 1857, 2034, 2043 |
| BfmI | CTRYAG | 4 cut(s) | 1197, 1271, 1344, 1942 |
| BfoI | RGCGCY | 3 cut(s) | 426, 1364, 1957 |
| BfuAI | ACCTGC | 1 cut(s) | 1706 |
| BfuI | GTATCC | 3 cut(s) | 162, 985, 1072 |
| BisI | GCNGC | 4 cut(s) | 88, 1197, 1406, 1807 |
| BlpI | GCTNAGC | 1 cut(s) | 1593 |
| BlsI | GCNGC | 4 cut(s) | 89, 1198, 1407, 1808 |
| Bme1390I | CCNGG | 3 cut(s) | 366, 1836, 1913 |
| Bme18I | GGWCC | 3 cut(s) | 180, 523, 1826 |
| BmeT110I | CYCGRG | 1 cut(s) | 1292 |
| BmgT120I | GGNCC | 4 cut(s) | 180, 523, 1746, 1826 |
| BmiI | GGNNCC | 5 cut(s) | 138, 525, 811, 1747, 1827 |
| BmrFI | CCNGG | 3 cut(s) | 366, 1836, 1913 |
| BmsI | GCATC | 3 cut(s) | 1289, 1330, 1378 |
| BmtI | GCTAGC | 1 cut(s) | 2037 |
| BpiI | GAAGAC | 1 cut(s) | 1109 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 720, 752, 1891, 1923, 1940, 1972 |
| Bpu10I | CCTNAGC | 1 cut(s) | 838 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1593 |
| BpuEI | CTTGAG | 2 cut(s) | 1869, 2061 |
| BsaBI | GATNNNNATC | 1 cut(s) | 141 |
| BsaI | GGTCTC | 2 cut(s) | 293, 941 |
| BsaJI | CCNNGG | 3 cut(s) | 183, 1911, 1912 |
| BsaWI | WCCGGW | 2 cut(s) | 320, 711 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 179, 209, 1368, 1398 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 533, 1276, 1763, 1796, 1835, 1841 |
| Bse118I | RCCGGY | 1 cut(s) | 755 |
| Bse1I | ACTGG | 3 cut(s) | 1155, 1242, 2030 |
| Bse21I | CCTNAGG | 1 cut(s) | 554 |
| Bse3DI | GCAATG | 1 cut(s) | 1620 |
| Bse8I | GATNNNNATC | 1 cut(s) | 141 |
| BseBI | CCWGG | 3 cut(s) | 366, 1836, 1913 |
| BseDI | CCNNGG | 3 cut(s) | 183, 1911, 1912 |
| BseGI | GGATG | 5 cut(s) | 244, 497, 811, 1053, 2010 |
| BseJI | GATNNNNATC | 1 cut(s) | 141 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 533, 1276, 1763, 1796, 1835, 1841 |
| BseMI | GCAATG | 1 cut(s) | 1620 |
| BseMII | CTCAG | 8 cut(s) | 236, 394, 529, 545, 952, 965, 1584, 1946 |
| BseNI | ACTGG | 3 cut(s) | 1155, 1242, 2030 |
| BseRI | GAGGAG | 2 cut(s) | 1529, 1964 |
| BseX3I | CGGCCG | 2 cut(s) | 88, 261 |
| BseXI | GCAGC | 2 cut(s) | 1183, 1818 |
| Bsh1236I | CGCG | 2 cut(s) | 87, 658 |
| Bsh1285I | CGRYCG | 2 cut(s) | 91, 264 |
| BsiEI | CGRYCG | 2 cut(s) | 91, 264 |
| BsiHKAI | GWGCWC | 3 cut(s) | 19, 1293, 2025 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1292 |
| BsiSI | CCGG | 5 cut(s) | 20, 321, 501, 712, 756 |
| BslFI | GGGAC | 2 cut(s) | 364, 1929 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 533, 1276, 1763, 1796, 1835, 1841 |
| BsmAI | GTCTC | 3 cut(s) | 293, 941, 1977 |
| BsmFI | GGGAC | 2 cut(s) | 364, 1929 |
| BsmI | GAATGC | 1 cut(s) | 1554 |
| Bso31I | GGTCTC | 2 cut(s) | 293, 941 |
| BsoBI | CYCGRG | 1 cut(s) | 1292 |
| Bsp1286I | GDGCHC | 3 cut(s) | 19, 1293, 2025 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1593 |
| Bsp68I | TCGCGA | 1 cut(s) | 658 |
| BspACI | CCGC | 4 cut(s) | 23, 53, 87, 1405 |
| BspCNI | CTCAG | 8 cut(s) | 235, 393, 528, 546, 951, 964, 1585, 1945 |
| BspFNI | CGCG | 2 cut(s) | 87, 658 |
| BspHI | TCATGA | 2 cut(s) | 1975, 2062 |
| BspLI | GGNNCC | 5 cut(s) | 138, 525, 811, 1747, 1827 |
| BspMAI | CTGCAG | 2 cut(s) | 1201, 1946 |
| BspMI | ACCTGC | 1 cut(s) | 1706 |
| BspOI | GCTAGC | 1 cut(s) | 2037 |
| BspPI | GGATC | 8 cut(s) | 131, 144, 212, 356, 580, 756, 1629, 1716 |
| BspTNI | GGTCTC | 2 cut(s) | 293, 941 |
| BsrDI | GCAATG | 1 cut(s) | 1620 |
| BsrFI | RCCGGY | 1 cut(s) | 755 |
| BsrI | ACTGG | 3 cut(s) | 1155, 1242, 2030 |
| BssAI | RCCGGY | 1 cut(s) | 755 |
| BssECI | CCNNGG | 3 cut(s) | 183, 1911, 1912 |
| BssT1I | CCWWGG | 1 cut(s) | 183 |
| Bst2UI | CCWGG | 3 cut(s) | 366, 1836, 1913 |
| BstC8I | GCNNGC | 5 cut(s) | 65, 550, 757, 2035, 2039 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1274 |
| BstF5I | GGATG | 5 cut(s) | 244, 497, 811, 1053, 2010 |
| BstFNI | CGCG | 2 cut(s) | 87, 658 |
| BstH2I | RGCGCY | 3 cut(s) | 426, 1364, 1957 |
| BstHHI | GCGC | 5 cut(s) | 87, 425, 1363, 1554, 1956 |
| BstMAI | GTCTC | 3 cut(s) | 293, 941, 1977 |
| BstMCI | CGRYCG | 2 cut(s) | 91, 264 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1031, 1397, 1560 |
| BstNI | CCWGG | 3 cut(s) | 366, 1836, 1913 |
| BstNSI | RCATGY | 4 cut(s) | 447, 668, 1088, 1663 |
| BstSCI | CCNGG | 3 cut(s) | 364, 1834, 1911 |
| BstSFI | CTRYAG | 4 cut(s) | 1197, 1271, 1344, 1942 |
| BstUI | CGCG | 2 cut(s) | 87, 658 |
| BstV1I | GCAGC | 2 cut(s) | 1183, 1818 |
| BstV2I | GAAGAC | 1 cut(s) | 1109 |
| BstX2I | RGATCY | 4 cut(s) | 136, 585, 748, 1708 |
| BstYI | RGATCY | 4 cut(s) | 136, 585, 748, 1708 |
| BstZI | CGGCCG | 2 cut(s) | 88, 261 |
| Bsu36I | CCTNAGG | 1 cut(s) | 554 |
| BsuI | GTATCC | 3 cut(s) | 162, 985, 1072 |
| BtsCI | GGATG | 5 cut(s) | 244, 497, 811, 1053, 2010 |
| BtsI | GCAGTG | 3 cut(s) | 675, 766, 1078 |
| BtsIMutI | CAGTG | 9 cut(s) | 230, 675, 766, 946, 1002, 1078, 1249, 1649, 2023 |
| BtuMI | TCGCGA | 1 cut(s) | 658 |
| BveI | ACCTGC | 1 cut(s) | 1706 |
| Cac8I | GCNNGC | 5 cut(s) | 65, 550, 757, 2035, 2039 |
| CciI | TCATGA | 2 cut(s) | 1975, 2062 |
| CfoI | GCGC | 5 cut(s) | 87, 425, 1363, 1554, 1956 |
| Cfr10I | RCCGGY | 1 cut(s) | 755 |
| Cfr13I | GGNCC | 4 cut(s) | 180, 523, 1746, 1826 |
| Csp6I | GTAC | 3 cut(s) | 228, 709, 1317 |
| CviQI | GTAC | 3 cut(s) | 228, 709, 1317 |
| DraIII | CACNNNGTG | 2 cut(s) | 359, 1006 |
| EaeI | YGGCCR | 4 cut(s) | 88, 261, 502, 1132 |
| EagI | CGGCCG | 2 cut(s) | 88, 261 |
| Ecl136II | GAGCTC | 2 cut(s) | 17, 1291 |
| EclXI | CGGCCG | 2 cut(s) | 88, 261 |
| Eco130I | CCWWGG | 1 cut(s) | 183 |
| Eco147I | AGGCCT | 1 cut(s) | 2041 |
| Eco24I | GRGCYC | 2 cut(s) | 19, 1293 |
| Eco31I | GGTCTC | 2 cut(s) | 293, 941 |
| Eco47I | GGWCC | 3 cut(s) | 180, 523, 1826 |
| Eco47III | AGCGCT | 2 cut(s) | 424, 1955 |
| Eco52I | CGGCCG | 2 cut(s) | 88, 261 |
| Eco53kI | GAGCTC | 2 cut(s) | 17, 1291 |
| Eco57I | CTGAAG | 2 cut(s) | 1103, 1974 |
| Eco81I | CCTNAGG | 1 cut(s) | 554 |
| Eco88I | CYCGRG | 1 cut(s) | 1292 |
| EcoICRI | GAGCTC | 2 cut(s) | 17, 1291 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1274 |
| EcoO109I | RGGNCCY | 2 cut(s) | 523, 1826 |
| EcoRII | CCWGG | 3 cut(s) | 364, 1834, 1911 |
| EcoT14I | CCWWGG | 1 cut(s) | 183 |
| EcoT22I | ATGCAT | 3 cut(s) | 214, 257, 1393 |
| EcoT38I | GRGCYC | 2 cut(s) | 19, 1293 |
| ErhI | CCWWGG | 1 cut(s) | 183 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 185, 217 |
| FaqI | GGGAC | 2 cut(s) | 364, 1929 |
| FauNDI | CATATG | 2 cut(s) | 310, 1506 |
| FblI | GTMKAC | 1 cut(s) | 243 |
| Fnu4HI | GCNGC | 4 cut(s) | 88, 1197, 1406, 1807 |
| FokI | GGATG | 5 cut(s) | 251, 484, 818, 1040, 2017 |
| FriOI | GRGCYC | 2 cut(s) | 19, 1293 |
| Fsp4HI | GCNGC | 4 cut(s) | 88, 1197, 1406, 1807 |
| FspBI | CTAG | 3 cut(s) | 1857, 2034, 2043 |
| FspI | TGCGCA | 1 cut(s) | 1553 |
| GlaI | GCGC | 5 cut(s) | 86, 424, 1362, 1553, 1955 |
| GluI | GCNGC | 4 cut(s) | 88, 1197, 1406, 1807 |
| HaeII | RGCGCY | 3 cut(s) | 426, 1364, 1957 |
| HapII | CCGG | 5 cut(s) | 20, 321, 501, 712, 756 |
| HhaI | GCGC | 5 cut(s) | 87, 425, 1363, 1554, 1956 |
| Hin6I | GCGC | 5 cut(s) | 85, 423, 1361, 1552, 1954 |
| HinP1I | GCGC | 5 cut(s) | 85, 423, 1361, 1552, 1954 |
| HincII | GTYRAC | 3 cut(s) | 985, 1453, 1496 |
| HindII | GTYRAC | 3 cut(s) | 985, 1453, 1496 |
| HinfI | GANTC | 7 cut(s) | 175, 369, 537, 715, 1163, 1686, 1728 |
| HpaI | GTTAAC | 1 cut(s) | 1453 |
| HpaII | CCGG | 5 cut(s) | 20, 321, 501, 712, 756 |
| HphI | GGTGA | 2 cut(s) | 37, 67 |
| Hpy166II | GTNNAC | 8 cut(s) | 228, 244, 764, 827, 866, 985, 1453, 1496 |
| Hpy188I | TCNGA | 7 cut(s) | 180, 518, 775, 954, 1093, 1122, 1924 |
| Hpy8I | GTNNAC | 8 cut(s) | 228, 244, 764, 827, 866, 985, 1453, 1496 |
| Hpy99I | CGWCG | 1 cut(s) | 621 |
| HpyAV | CCTTC | 6 cut(s) | 871, 1186, 1270, 1839, 1969, 1998 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1031, 1397, 1560 |
| HspAI | GCGC | 5 cut(s) | 85, 423, 1361, 1552, 1954 |
| KroI | GCCGGC | 1 cut(s) | 755 |
| KroNI | GCCGGC | 1 cut(s) | 757 |
| KspAI | GTTAAC | 1 cut(s) | 1453 |
| LmnI | GCTCC | 6 cut(s) | 22, 82, 809, 1780, 1951, 1961 |
| Lsp1109I | GCAGC | 2 cut(s) | 1183, 1818 |
| LweI | GCATC | 3 cut(s) | 1289, 1330, 1378 |
| MaeI | CTAG | 3 cut(s) | 1857, 2034, 2043 |
| MaeIII | GTNAC | 5 cut(s) | 439, 1310, 1430, 1526, 1799 |
| MboII | GAAGA | 5 cut(s) | 40, 202, 645, 1109, 1751 |
| MflI | RGATCY | 4 cut(s) | 136, 585, 748, 1708 |
| MhlI | GDGCHC | 3 cut(s) | 19, 1293, 2025 |
| MlsI | TGGCCA | 1 cut(s) | 1134 |
| MluCI | AATT | 6 cut(s) | 232, 431, 684, 911, 946, 1601 |
| MluNI | TGGCCA | 1 cut(s) | 1134 |
| MlyI | GAGTC | 2 cut(s) | 363, 1680 |
| MmeI | TCCRAC | 2 cut(s) | 114, 1525 |
| Mox20I | TGGCCA | 1 cut(s) | 1134 |
| Mph1103I | ATGCAT | 3 cut(s) | 214, 257, 1393 |
| MroNI | GCCGGC | 1 cut(s) | 755 |
| MscI | TGGCCA | 1 cut(s) | 1134 |
| MseI | TTAA | 8 cut(s) | 72, 599, 687, 800, 819, 1452, 1632, 1967 |
| Msp20I | TGGCCA | 1 cut(s) | 1134 |
| MspA1I | CMGCKG | 1 cut(s) | 578 |
| MspI | CCGG | 5 cut(s) | 20, 321, 501, 712, 756 |
| MspR9I | CCNGG | 3 cut(s) | 366, 1836, 1913 |
| Mva1269I | GAATGC | 1 cut(s) | 1554 |
| MvaI | CCWGG | 3 cut(s) | 366, 1836, 1913 |
| MvnI | CGCG | 2 cut(s) | 87, 658 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1031, 1397, 1560 |
| NaeI | GCCGGC | 1 cut(s) | 757 |
| NdeI | CATATG | 2 cut(s) | 310, 1506 |
| NgoMIV | GCCGGC | 1 cut(s) | 755 |
| NheI | GCTAGC | 1 cut(s) | 2033 |
| NlaIV | GGNNCC | 5 cut(s) | 138, 525, 811, 1747, 1827 |
| NmeAIII | GCCGAG | 1 cut(s) | 30 |
| NmuCI | GTSAC | 1 cut(s) | 1430 |
| NruI | TCGCGA | 1 cut(s) | 658 |
| NsbI | TGCGCA | 1 cut(s) | 1553 |
| NsiI | ATGCAT | 3 cut(s) | 214, 257, 1393 |
| NspI | RCATGY | 4 cut(s) | 447, 668, 1088, 1663 |
| PaeR7I | CTCGAG | 1 cut(s) | 1292 |
| PagI | TCATGA | 2 cut(s) | 1975, 2062 |
| PasI | CCCWGGG | 1 cut(s) | 1912 |
| PceI | AGGCCT | 1 cut(s) | 2041 |
| PciI | ACATGT | 3 cut(s) | 443, 1084, 1659 |
| PctI | GAATGC | 1 cut(s) | 1554 |
| PdiI | GCCGGC | 1 cut(s) | 757 |
| PfeI | GAWTC | 5 cut(s) | 175, 537, 715, 1163, 1728 |
| PfoI | TCCNGGA | 1 cut(s) | 364 |
| PkrI | GCNGC | 4 cut(s) | 89, 1198, 1407, 1808 |
| PleI | GAGTC | 2 cut(s) | 363, 1680 |
| PpsI | GAGTC | 2 cut(s) | 363, 1680 |
| PpuMI | RGGWCCY | 2 cut(s) | 523, 1826 |
| PscI | ACATGT | 3 cut(s) | 443, 1084, 1659 |
| Psp124BI | GAGCTC | 2 cut(s) | 19, 1293 |
| Psp5II | RGGWCCY | 2 cut(s) | 523, 1826 |
| Psp6I | CCWGG | 3 cut(s) | 364, 1834, 1911 |
| PspGI | CCWGG | 3 cut(s) | 364, 1834, 1911 |
| PspN4I | GGNNCC | 5 cut(s) | 138, 525, 811, 1747, 1827 |
| PspPI | GGNCC | 4 cut(s) | 180, 523, 1746, 1826 |
| PspPPI | RGGWCCY | 2 cut(s) | 523, 1826 |
| PspXI | VCTCGAGB | 1 cut(s) | 1292 |
| PstI | CTGCAG | 2 cut(s) | 1201, 1946 |
| PsuI | RGATCY | 4 cut(s) | 136, 585, 748, 1708 |
| PvuII | CAGCTG | 1 cut(s) | 578 |
| RruI | TCGCGA | 1 cut(s) | 658 |
| RsaI | GTAC | 3 cut(s) | 229, 710, 1318 |
| RsaNI | GTAC | 3 cut(s) | 228, 709, 1317 |
| SacI | GAGCTC | 2 cut(s) | 19, 1293 |
| SaqAI | TTAA | 8 cut(s) | 72, 599, 687, 800, 819, 1452, 1632, 1967 |
| SatI | GCNGC | 4 cut(s) | 88, 1197, 1406, 1807 |
| Sau96I | GGNCC | 4 cut(s) | 180, 523, 1746, 1826 |
| SchI | GAGTC | 2 cut(s) | 363, 1680 |
| ScrFI | CCNGG | 3 cut(s) | 366, 1836, 1913 |
| SduI | GDGCHC | 3 cut(s) | 19, 1293, 2025 |
| SfaNI | GCATC | 3 cut(s) | 1289, 1330, 1378 |
| SfcI | CTRYAG | 4 cut(s) | 1197, 1271, 1344, 1942 |
| Sfr274I | CTCGAG | 1 cut(s) | 1292 |
| SinI | GGWCC | 3 cut(s) | 180, 523, 1826 |
| SlaI | CTCGAG | 1 cut(s) | 1292 |
| SmlI | CTYRAG | 3 cut(s) | 1292, 1884, 2076 |
| SmoI | CTYRAG | 3 cut(s) | 1292, 1884, 2076 |
| Sse9I | AATT | 6 cut(s) | 232, 431, 684, 911, 946, 1601 |
| SseBI | AGGCCT | 1 cut(s) | 2041 |
| SsiI | CCGC | 4 cut(s) | 23, 53, 87, 1405 |
| SspI | AATATT | 2 cut(s) | 817, 1225 |
| SspMI | CTAG | 3 cut(s) | 1857, 2034, 2043 |
| SstI | GAGCTC | 2 cut(s) | 19, 1293 |
| StuI | AGGCCT | 1 cut(s) | 2041 |
| StyD4I | CCNGG | 3 cut(s) | 364, 1834, 1911 |
| StyI | CCWWGG | 1 cut(s) | 183 |
| TaqI | TCGA | 6 cut(s) | 149, 1030, 1287, 1293, 1689, 1898 |
| TasI | AATT | 6 cut(s) | 232, 431, 684, 911, 946, 1601 |
| TauI | GCSGC | 2 cut(s) | 90, 1408 |
| TfiI | GAWTC | 5 cut(s) | 175, 537, 715, 1163, 1728 |
| Tru1I | TTAA | 8 cut(s) | 72, 599, 687, 800, 819, 1452, 1632, 1967 |
| Tru9I | TTAA | 8 cut(s) | 72, 599, 687, 800, 819, 1452, 1632, 1967 |
| TscAI | CASTG | 9 cut(s) | 230, 675, 766, 946, 1009, 1078, 1249, 1656, 2030 |
| TseFI | GTSAC | 1 cut(s) | 1430 |
| TseI | GCWGC | 2 cut(s) | 1196, 1806 |
| Tsp45I | GTSAC | 1 cut(s) | 1430 |
| TspDTI | ATGAA | 7 cut(s) | 272, 444, 483, 1203, 1251, 1455, 1707 |
| TspGWI | ACGGA | 2 cut(s) | 634, 650 |
| TspRI | CASTG | 9 cut(s) | 230, 675, 766, 946, 1009, 1078, 1249, 1656, 2030 |
| VpaK11BI | GGWCC | 3 cut(s) | 180, 523, 1826 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1274 |
| XapI | RAATTY | 1 cut(s) | 431 |
| XbaI | TCTAGA | 1 cut(s) | 1856 |
| XceI | RCATGY | 4 cut(s) | 447, 668, 1088, 1663 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1064 |
| XhoI | CTCGAG | 1 cut(s) | 1292 |
| XmiI | GTMKAC | 1 cut(s) | 243 |
| XspI | CTAG | 3 cut(s) | 1857, 2034, 2043 |
| Zsp2I | ATGCAT | 3 cut(s) | 214, 257, 1393 |