Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 517 |
| AccI | GTMKAC | 1 cut(s) | 309 |
| AclWI | GGATC | 3 cut(s) | 422, 583, 596 |
| AcoI | YGGCCR | 2 cut(s) | 567, 626 |
| AcsI | RAATTY | 1 cut(s) | 496 |
| AdeI | CACNNNGTG | 1 cut(s) | 65 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 83, 598, 624 |
| AflII | CTTAAG | 1 cut(s) | 53 |
| AgsI | TTSAA | 4 cut(s) | 34, 502, 523, 556 |
| AhlI | ACTAGT | 1 cut(s) | 658 |
| AjnI | CCWGG | 1 cut(s) | 16 |
| AluBI | AGCT | 4 cut(s) | 256, 268, 289, 488 |
| AluI | AGCT | 4 cut(s) | 256, 268, 289, 488 |
| Alw21I | GWGCWC | 2 cut(s) | 70, 490 |
| Alw26I | GTCTC | 2 cut(s) | 152, 359 |
| AlwI | GGATC | 3 cut(s) | 422, 583, 596 |
| Ama87I | CYCGRG | 1 cut(s) | 431 |
| AoxI | GGCC | 3 cut(s) | 567, 615, 626 |
| ApeKI | GCWGC | 1 cut(s) | 265 |
| ApoI | RAATTY | 1 cut(s) | 496 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 255 |
| AspS9I | GGNCC | 2 cut(s) | 46, 74 |
| AsuC2I | CCSGG | 2 cut(s) | 432, 433 |
| AsuHPI | GGTGA | 1 cut(s) | 152 |
| AvaI | CYCGRG | 1 cut(s) | 431 |
| AvaII | GGWCC | 2 cut(s) | 46, 74 |
| BalI | TGGCCA | 1 cut(s) | 628 |
| BamHI | GGATCC | 1 cut(s) | 588 |
| BanII | GRGCYC | 2 cut(s) | 438, 490 |
| BbsI | GAAGAC | 1 cut(s) | 94 |
| Bbv12I | GWGCWC | 2 cut(s) | 70, 490 |
| BbvI | GCAGC | 1 cut(s) | 277 |
| BccI | CCATC | 1 cut(s) | 98 |
| BceAI | ACGGC | 1 cut(s) | 9 |
| BciT130I | CCWGG | 1 cut(s) | 18 |
| BclI | TGATCA | 1 cut(s) | 168 |
| BcnI | CCSGG | 2 cut(s) | 432, 433 |
| BcoDI | GTCTC | 2 cut(s) | 152, 359 |
| BcuI | ACTAGT | 1 cut(s) | 658 |
| BfaI | CTAG | 2 cut(s) | 275, 659 |
| BfrI | CTTAAG | 1 cut(s) | 53 |
| BfuAI | ACCTGC | 1 cut(s) | 517 |
| BisI | GCNGC | 1 cut(s) | 266 |
| BlsI | GCNGC | 1 cut(s) | 267 |
| Bme1390I | CCNGG | 3 cut(s) | 18, 432, 433 |
| Bme18I | GGWCC | 2 cut(s) | 46, 74 |
| BmeT110I | CYCGRG | 1 cut(s) | 431 |
| BmgT120I | GGNCC | 2 cut(s) | 46, 74 |
| BmiI | GGNNCC | 4 cut(s) | 48, 75, 76, 590 |
| BmrFI | CCNGG | 3 cut(s) | 18, 432, 433 |
| BmsI | GCATC | 1 cut(s) | 648 |
| BpiI | GAAGAC | 1 cut(s) | 94 |
| Bpu10I | CCTNAGC | 1 cut(s) | 69 |
| BpuEI | CTTGAG | 1 cut(s) | 279 |
| BpuMI | CCSGG | 2 cut(s) | 432, 433 |
| BsaI | GGTCTC | 1 cut(s) | 359 |
| BsaJI | CCNNGG | 3 cut(s) | 16, 25, 431 |
| BsaWI | WCCGGW | 1 cut(s) | 386 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 83, 598, 624 |
| BseBI | CCWGG | 1 cut(s) | 18 |
| BseDI | CCNNGG | 3 cut(s) | 16, 25, 431 |
| BseGI | GGATG | 4 cut(s) | 179, 310, 562, 639 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 83, 598, 624 |
| BseMII | CTCAG | 3 cut(s) | 76, 83, 594 |
| BseXI | GCAGC | 1 cut(s) | 277 |
| BshFI | GGCC | 3 cut(s) | 569, 617, 628 |
| BsiHKAI | GWGCWC | 2 cut(s) | 70, 490 |
| BsiHKCI | CYCGRG | 1 cut(s) | 431 |
| BsiSI | CCGG | 3 cut(s) | 387, 432, 566 |
| BslFI | GGGAC | 2 cut(s) | 87, 430 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 83, 598, 624 |
| BsmAI | GTCTC | 2 cut(s) | 152, 359 |
| BsmFI | GGGAC | 2 cut(s) | 87, 430 |
| BsnI | GGCC | 3 cut(s) | 569, 617, 628 |
| Bso31I | GGTCTC | 1 cut(s) | 359 |
| BsoBI | CYCGRG | 1 cut(s) | 431 |
| Bsp1286I | GDGCHC | 3 cut(s) | 70, 438, 490 |
| Bsp143I | GATC | 5 cut(s) | 168, 333, 427, 466, 588 |
| BspANI | GGCC | 3 cut(s) | 569, 617, 628 |
| BspCNI | CTCAG | 3 cut(s) | 75, 82, 593 |
| BspLI | GGNNCC | 4 cut(s) | 48, 75, 76, 590 |
| BspMI | ACCTGC | 1 cut(s) | 517 |
| BspPI | GGATC | 3 cut(s) | 422, 583, 596 |
| BspQI | GCTCTTC | 1 cut(s) | 246 |
| BspTI | CTTAAG | 1 cut(s) | 53 |
| BspTNI | GGTCTC | 1 cut(s) | 359 |
| BssECI | CCNNGG | 3 cut(s) | 16, 25, 431 |
| BssMI | GATC | 5 cut(s) | 168, 333, 427, 466, 588 |
| Bst2UI | CCWGG | 1 cut(s) | 18 |
| Bst4CI | ACNGT | 1 cut(s) | 367 |
| Bst6I | CTCTTC | 1 cut(s) | 246 |
| BstAFI | CTTAAG | 1 cut(s) | 53 |
| BstC8I | GCNNGC | 2 cut(s) | 222, 615 |
| BstDEI | CTNAG | 5 cut(s) | 62, 69, 446, 580, 632 |
| BstDSI | CCRYGG | 1 cut(s) | 25 |
| BstF5I | GGATG | 4 cut(s) | 179, 310, 562, 639 |
| BstKTI | GATC | 5 cut(s) | 171, 336, 430, 469, 591 |
| BstMAI | GTCTC | 2 cut(s) | 152, 359 |
| BstMBI | GATC | 5 cut(s) | 168, 333, 427, 466, 588 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 262 |
| BstNI | CCWGG | 1 cut(s) | 18 |
| BstSCI | CCNGG | 3 cut(s) | 16, 430, 431 |
| BstV1I | GCAGC | 1 cut(s) | 277 |
| BstV2I | GAAGAC | 1 cut(s) | 94 |
| BstX2I | RGATCY | 1 cut(s) | 588 |
| BstYI | RGATCY | 1 cut(s) | 588 |
| BsuRI | GGCC | 3 cut(s) | 569, 617, 628 |
| BtgI | CCRYGG | 1 cut(s) | 25 |
| BtsCI | GGATG | 4 cut(s) | 179, 310, 562, 639 |
| BtsI | GCAGTG | 1 cut(s) | 115 |
| BtsIMutI | CAGTG | 2 cut(s) | 70, 115 |
| BveI | ACCTGC | 1 cut(s) | 517 |
| Cac8I | GCNNGC | 2 cut(s) | 222, 615 |
| Cfr13I | GGNCC | 2 cut(s) | 46, 74 |
| Cfr9I | CCCGGG | 1 cut(s) | 431 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 645, 680 |
| CviAII | CATG | 3 cut(s) | 186, 350, 439 |
| CviJI | RGCY | 8 cut(s) | 256, 268, 289, 436, 488, 569, 617, 628 |
| CviKI_1 | RGCY | 8 cut(s) | 256, 268, 289, 436, 488, 569, 617, 628 |
| DdeI | CTNAG | 5 cut(s) | 62, 69, 446, 580, 632 |
| DpnI | GATC | 5 cut(s) | 170, 335, 429, 468, 590 |
| DpnII | GATC | 5 cut(s) | 168, 333, 427, 466, 588 |
| DraIII | CACNNNGTG | 1 cut(s) | 65 |
| EaeI | YGGCCR | 2 cut(s) | 567, 626 |
| Eam1104I | CTCTTC | 1 cut(s) | 246 |
| EarI | CTCTTC | 1 cut(s) | 246 |
| Ecl136II | GAGCTC | 1 cut(s) | 488 |
| Eco24I | GRGCYC | 2 cut(s) | 438, 490 |
| Eco31I | GGTCTC | 1 cut(s) | 359 |
| Eco47I | GGWCC | 2 cut(s) | 46, 74 |
| Eco53kI | GAGCTC | 1 cut(s) | 488 |
| Eco88I | CYCGRG | 1 cut(s) | 431 |
| EcoICRI | GAGCTC | 1 cut(s) | 488 |
| EcoO109I | RGGNCCY | 2 cut(s) | 46, 74 |
| EcoRII | CCWGG | 1 cut(s) | 16 |
| EcoT22I | ATGCAT | 1 cut(s) | 323 |
| EcoT38I | GRGCYC | 2 cut(s) | 438, 490 |
| FaeI | CATG | 3 cut(s) | 189, 353, 442 |
| FaqI | GGGAC | 2 cut(s) | 87, 430 |
| FatI | CATG | 3 cut(s) | 185, 349, 438 |
| FbaI | TGATCA | 1 cut(s) | 168 |
| FblI | GTMKAC | 1 cut(s) | 309 |
| Fnu4HI | GCNGC | 1 cut(s) | 266 |
| FokI | GGATG | 4 cut(s) | 166, 317, 549, 626 |
| FriOI | GRGCYC | 2 cut(s) | 438, 490 |
| Fsp4HI | GCNGC | 1 cut(s) | 266 |
| FspBI | CTAG | 2 cut(s) | 275, 659 |
| GluI | GCNGC | 1 cut(s) | 266 |
| HaeIII | GGCC | 3 cut(s) | 569, 617, 628 |
| HapII | CCGG | 3 cut(s) | 387, 432, 566 |
| Hin1II | CATG | 3 cut(s) | 189, 353, 442 |
| HinfI | GANTC | 2 cut(s) | 30, 602 |
| HpaII | CCGG | 3 cut(s) | 387, 432, 566 |
| HphI | GGTGA | 1 cut(s) | 152 |
| Hpy166II | GTNNAC | 2 cut(s) | 191, 310 |
| Hpy188I | TCNGA | 3 cut(s) | 471, 492, 583 |
| Hpy188III | TCNNGA | 3 cut(s) | 34, 520, 592 |
| Hpy8I | GTNNAC | 2 cut(s) | 191, 310 |
| HpyCH4III | ACNGT | 1 cut(s) | 367 |
| HpyCH4V | TGCA | 5 cut(s) | 241, 321, 362, 454, 639 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 262 |
| HpyF3I | CTNAG | 5 cut(s) | 62, 69, 446, 580, 632 |
| Hsp92II | CATG | 3 cut(s) | 189, 353, 442 |
| KflI | GGGWCCC | 1 cut(s) | 74 |
| Ksp22I | TGATCA | 1 cut(s) | 168 |
| Kzo9I | GATC | 5 cut(s) | 168, 333, 427, 466, 588 |
| LguI | GCTCTTC | 1 cut(s) | 246 |
| LmnI | GCTCC | 1 cut(s) | 517 |
| Lsp1109I | GCAGC | 1 cut(s) | 277 |
| LweI | GCATC | 1 cut(s) | 648 |
| MaeI | CTAG | 2 cut(s) | 275, 659 |
| MaeIII | GTNAC | 4 cut(s) | 5, 57, 110, 504 |
| MalI | GATC | 5 cut(s) | 170, 335, 429, 468, 590 |
| MboI | GATC | 5 cut(s) | 168, 333, 427, 466, 588 |
| MboII | GAAGA | 3 cut(s) | 99, 241, 263 |
| MflI | RGATCY | 1 cut(s) | 588 |
| MhlI | GDGCHC | 3 cut(s) | 70, 438, 490 |
| MlsI | TGGCCA | 1 cut(s) | 628 |
| MluCI | AATT | 1 cut(s) | 496 |
| MluNI | TGGCCA | 1 cut(s) | 628 |
| MnlI | CCTC | 4 cut(s) | 193, 229, 579, 628 |
| Mox20I | TGGCCA | 1 cut(s) | 628 |
| Mph1103I | ATGCAT | 1 cut(s) | 323 |
| MroXI | GAANNNNTTC | 1 cut(s) | 255 |
| MscI | TGGCCA | 1 cut(s) | 628 |
| MseI | TTAA | 3 cut(s) | 54, 648, 706 |
| MslI | CAYNNNNRTG | 1 cut(s) | 184 |
| Msp20I | TGGCCA | 1 cut(s) | 628 |
| MspCI | CTTAAG | 1 cut(s) | 53 |
| MspI | CCGG | 3 cut(s) | 387, 432, 566 |
| MspR9I | CCNGG | 3 cut(s) | 18, 432, 433 |
| MvaI | CCWGG | 1 cut(s) | 18 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 262 |
| NciI | CCSGG | 2 cut(s) | 432, 433 |
| NdeII | GATC | 5 cut(s) | 168, 333, 427, 466, 588 |
| NlaIII | CATG | 3 cut(s) | 189, 353, 442 |
| NlaIV | GGNNCC | 4 cut(s) | 48, 75, 76, 590 |
| NmuCI | GTSAC | 2 cut(s) | 57, 110 |
| NsiI | ATGCAT | 1 cut(s) | 323 |
| PciSI | GCTCTTC | 1 cut(s) | 246 |
| PdmI | GAANNNNTTC | 1 cut(s) | 255 |
| PfeI | GAWTC | 2 cut(s) | 30, 602 |
| PkrI | GCNGC | 1 cut(s) | 267 |
| PpuMI | RGGWCCY | 2 cut(s) | 46, 74 |
| Psp124BI | GAGCTC | 1 cut(s) | 490 |
| Psp5II | RGGWCCY | 2 cut(s) | 46, 74 |
| Psp6I | CCWGG | 1 cut(s) | 16 |
| PspGI | CCWGG | 1 cut(s) | 16 |
| PspN4I | GGNNCC | 4 cut(s) | 48, 75, 76, 590 |
| PspPI | GGNCC | 2 cut(s) | 46, 74 |
| PspPPI | RGGWCCY | 2 cut(s) | 46, 74 |
| PsuI | RGATCY | 1 cut(s) | 588 |
| RseI | CAYNNNNRTG | 1 cut(s) | 184 |
| SacI | GAGCTC | 1 cut(s) | 490 |
| SapI | GCTCTTC | 1 cut(s) | 246 |
| SaqAI | TTAA | 3 cut(s) | 54, 648, 706 |
| SatI | GCNGC | 1 cut(s) | 266 |
| Sau3AI | GATC | 5 cut(s) | 168, 333, 427, 466, 588 |
| Sau96I | GGNCC | 2 cut(s) | 46, 74 |
| ScrFI | CCNGG | 3 cut(s) | 18, 432, 433 |
| SduI | GDGCHC | 3 cut(s) | 70, 438, 490 |
| SetI | ASST | 7 cut(s) | 142, 240, 258, 270, 291, 490, 511 |
| SfaNI | GCATC | 1 cut(s) | 648 |
| SinI | GGWCC | 2 cut(s) | 46, 74 |
| SmaI | CCCGGG | 1 cut(s) | 433 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 184 |
| SmlI | CTYRAG | 2 cut(s) | 53, 294 |
| SmoI | CTYRAG | 2 cut(s) | 53, 294 |
| SpeI | ACTAGT | 1 cut(s) | 658 |
| Sse9I | AATT | 1 cut(s) | 496 |
| SspI | AATATT | 1 cut(s) | 462 |
| SspMI | CTAG | 2 cut(s) | 275, 659 |
| SstI | GAGCTC | 1 cut(s) | 490 |
| StyD4I | CCNGG | 3 cut(s) | 16, 430, 431 |
| TaaI | ACNGT | 1 cut(s) | 367 |
| TasI | AATT | 1 cut(s) | 496 |
| TfiI | GAWTC | 2 cut(s) | 30, 602 |
| Tru1I | TTAA | 3 cut(s) | 54, 648, 706 |
| Tru9I | TTAA | 3 cut(s) | 54, 648, 706 |
| TscAI | CASTG | 2 cut(s) | 70, 115 |
| TseFI | GTSAC | 2 cut(s) | 57, 110 |
| TseI | GCWGC | 1 cut(s) | 265 |
| Tsp45I | GTSAC | 2 cut(s) | 57, 110 |
| TspDTI | ATGAA | 2 cut(s) | 338, 509 |
| TspGWI | ACGGA | 2 cut(s) | 208, 319 |
| TspMI | CCCGGG | 1 cut(s) | 431 |
| TspRI | CASTG | 2 cut(s) | 70, 115 |
| Vha464I | CTTAAG | 1 cut(s) | 53 |
| VpaK11BI | GGWCC | 2 cut(s) | 46, 74 |
| XapI | RAATTY | 1 cut(s) | 496 |
| XmaI | CCCGGG | 1 cut(s) | 431 |
| XmiI | GTMKAC | 1 cut(s) | 309 |
| XmnI | GAANNNNTTC | 1 cut(s) | 255 |
| XspI | CTAG | 2 cut(s) | 275, 659 |
| Zsp2I | ATGCAT | 1 cut(s) | 323 |