Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1553 |
| Acc36I | ACCTGC | 1 cut(s) | 452 |
| AccB1I | GGYRCC | 1 cut(s) | 809 |
| AccI | GTMKAC | 1 cut(s) | 243 |
| AccII | CGCG | 2 cut(s) | 87, 93 |
| AciI | CCGC | 4 cut(s) | 23, 87, 91, 1315 |
| AclWI | GGATC | 8 cut(s) | 212, 356, 518, 531, 580, 756, 1629, 1716 |
| AcoI | YGGCCR | 4 cut(s) | 24, 88, 502, 1132 |
| AcsI | RAATTY | 1 cut(s) | 431 |
| AcuI | CTGAAG | 1 cut(s) | 1103 |
| AdeI | CACNNNGTG | 1 cut(s) | 1006 |
| AfaI | GTAC | 3 cut(s) | 229, 710, 894 |
| AfeI | AGCGCT | 1 cut(s) | 1955 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 533, 1276, 1763, 1796, 1841 |
| AflIII | ACRYGT | 2 cut(s) | 1084, 1659 |
| AgeI | ACCGGT | 1 cut(s) | 1183 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1409 |
| AjnI | CCWGG | 5 cut(s) | 159, 364, 1834, 1911, 2098 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2080, 2112 |
| AluBI | AGCT | 9 cut(s) | 17, 423, 578, 1154, 1210, 1291, 1333, 1445, 1597 |
| AluI | AGCT | 9 cut(s) | 17, 423, 578, 1154, 1210, 1291, 1333, 1445, 1597 |
| Alw21I | GWGCWC | 3 cut(s) | 19, 425, 2025 |
| Alw26I | GTCTC | 2 cut(s) | 293, 1977 |
| AlwI | GGATC | 8 cut(s) | 212, 356, 518, 531, 580, 756, 1629, 1716 |
| Aor51HI | AGCGCT | 1 cut(s) | 1955 |
| ApeKI | GCWGC | 2 cut(s) | 1196, 1806 |
| ApoI | RAATTY | 1 cut(s) | 431 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 865, 897 |
| AsiGI | ACCGGT | 1 cut(s) | 1183 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1251 |
| AspLEI | GCGC | 3 cut(s) | 87, 1554, 1956 |
| AspS9I | GGNCC | 1 cut(s) | 1746 |
| AsuHPI | GGTGA | 1 cut(s) | 1779 |
| AxyI | CCTNAGG | 1 cut(s) | 554 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 1071, 1071, 1104, 1104 |
| BamHI | GGATCC | 1 cut(s) | 523 |
| BanI | GGYRCC | 1 cut(s) | 809 |
| BanII | GRGCYC | 3 cut(s) | 19, 372, 425 |
| BbsI | GAAGAC | 1 cut(s) | 1109 |
| Bbv12I | GWGCWC | 3 cut(s) | 19, 425, 2025 |
| BbvI | GCAGC | 2 cut(s) | 1183, 1818 |
| BccI | CCATC | 6 cut(s) | 800, 926, 1367, 1529, 1669, 1736 |
| BceAI | ACGGC | 4 cut(s) | 52, 1119, 1421, 1819 |
| BciT130I | CCWGG | 5 cut(s) | 161, 366, 1836, 1913, 2100 |
| BciVI | GTATCC | 3 cut(s) | 130, 162, 985 |
| BclI | TGATCA | 1 cut(s) | 205 |
| BcoDI | GTCTC | 2 cut(s) | 293, 1977 |
| BfaI | CTAG | 4 cut(s) | 1151, 1857, 2043, 2106 |
| BfmI | CTRYAG | 3 cut(s) | 1197, 1271, 1344 |
| BfoI | RGCGCY | 1 cut(s) | 1957 |
| BfuAI | ACCTGC | 1 cut(s) | 452 |
| BfuI | GTATCC | 3 cut(s) | 130, 162, 985 |
| BisI | GCNGC | 5 cut(s) | 24, 88, 91, 1197, 1807 |
| BlpI | GCTNAGC | 1 cut(s) | 1593 |
| BlsI | GCNGC | 5 cut(s) | 25, 89, 92, 1198, 1808 |
| Bme1390I | CCNGG | 5 cut(s) | 161, 366, 1836, 1913, 2100 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1409 |
| BmgT120I | GGNCC | 1 cut(s) | 1746 |
| BmiI | GGNNCC | 3 cut(s) | 525, 811, 1747 |
| BmrFI | CCNGG | 5 cut(s) | 161, 366, 1836, 1913, 2100 |
| BmsI | GCATC | 2 cut(s) | 1289, 1330 |
| BpiI | GAAGAC | 1 cut(s) | 1109 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 720, 752, 1940, 1972 |
| BpmI | CTGGAG | 1 cut(s) | 182 |
| Bpu10I | CCTNAGC | 1 cut(s) | 838 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1593 |
| BpuEI | CTTGAG | 3 cut(s) | 1313, 1869, 2061 |
| BsaBI | GATNNNNATC | 2 cut(s) | 141, 2070 |
| BsaI | GGTCTC | 1 cut(s) | 293 |
| BsaJI | CCNNGG | 4 cut(s) | 183, 365, 1911, 1912 |
| BsaWI | WCCGGW | 3 cut(s) | 320, 711, 1183 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 179, 209, 1368, 1398 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 533, 1276, 1763, 1796, 1841 |
| Bse118I | RCCGGY | 2 cut(s) | 755, 1183 |
| Bse1I | ACTGG | 2 cut(s) | 1242, 2030 |
| Bse21I | CCTNAGG | 1 cut(s) | 554 |
| Bse3DI | GCAATG | 1 cut(s) | 1620 |
| Bse8I | GATNNNNATC | 2 cut(s) | 141, 2070 |
| BseBI | CCWGG | 5 cut(s) | 161, 366, 1836, 1913, 2100 |
| BseDI | CCNNGG | 4 cut(s) | 183, 365, 1911, 1912 |
| BseGI | GGATG | 7 cut(s) | 244, 497, 811, 1053, 1904, 2010, 2071 |
| BseJI | GATNNNNATC | 2 cut(s) | 141, 2070 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 533, 1276, 1763, 1796, 1841 |
| BseMI | GCAATG | 1 cut(s) | 1620 |
| BseMII | CTCAG | 6 cut(s) | 394, 529, 545, 929, 965, 1584 |
| BseNI | ACTGG | 2 cut(s) | 1242, 2030 |
| BseRI | GAGGAG | 2 cut(s) | 1529, 1964 |
| BseX3I | CGGCCG | 1 cut(s) | 88 |
| BseXI | GCAGC | 2 cut(s) | 1183, 1818 |
| Bsh1236I | CGCG | 2 cut(s) | 87, 93 |
| Bsh1285I | CGRYCG | 2 cut(s) | 91, 662 |
| BshNI | GGYRCC | 1 cut(s) | 809 |
| BshTI | ACCGGT | 1 cut(s) | 1183 |
| BsiEI | CGRYCG | 2 cut(s) | 91, 662 |
| BsiHKAI | GWGCWC | 3 cut(s) | 19, 425, 2025 |
| BsiSI | CCGG | 6 cut(s) | 20, 321, 501, 712, 756, 1184 |
| BslFI | GGGAC | 3 cut(s) | 364, 1679, 1929 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 533, 1276, 1763, 1796, 1841 |
| BsmAI | GTCTC | 2 cut(s) | 293, 1977 |
| BsmFI | GGGAC | 3 cut(s) | 364, 1679, 1929 |
| BsmI | GAATGC | 1 cut(s) | 1554 |
| Bso31I | GGTCTC | 1 cut(s) | 293 |
| Bsp1286I | GDGCHC | 4 cut(s) | 19, 372, 425, 2025 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1593 |
| BspACI | CCGC | 4 cut(s) | 23, 87, 91, 1315 |
| BspCNI | CTCAG | 6 cut(s) | 393, 528, 546, 930, 964, 1585 |
| BspFNI | CGCG | 2 cut(s) | 87, 93 |
| BspHI | TCATGA | 1 cut(s) | 1975 |
| BspLI | GGNNCC | 3 cut(s) | 525, 811, 1747 |
| BspMAI | CTGCAG | 1 cut(s) | 1201 |
| BspMI | ACCTGC | 1 cut(s) | 452 |
| BspPI | GGATC | 8 cut(s) | 212, 356, 518, 531, 580, 756, 1629, 1716 |
| BspT107I | GGYRCC | 1 cut(s) | 809 |
| BspTNI | GGTCTC | 1 cut(s) | 293 |
| BsrDI | GCAATG | 1 cut(s) | 1620 |
| BsrFI | RCCGGY | 2 cut(s) | 755, 1183 |
| BsrI | ACTGG | 2 cut(s) | 1242, 2030 |
| BssAI | RCCGGY | 2 cut(s) | 755, 1183 |
| BssECI | CCNNGG | 4 cut(s) | 183, 365, 1911, 1912 |
| BssT1I | CCWWGG | 1 cut(s) | 183 |
| Bst2UI | CCWGG | 5 cut(s) | 161, 366, 1836, 1913, 2100 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 670 |
| BstC8I | GCNNGC | 6 cut(s) | 65, 550, 666, 757, 2035, 2039 |
| BstDEI | CTNAG | 9 cut(s) | 380, 515, 554, 838, 938, 951, 1172, 1559, 1593 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1274 |
| BstF5I | GGATG | 7 cut(s) | 244, 497, 811, 1053, 1904, 2010, 2071 |
| BstFNI | CGCG | 2 cut(s) | 87, 93 |
| BstH2I | RGCGCY | 1 cut(s) | 1957 |
| BstHHI | GCGC | 3 cut(s) | 87, 1554, 1956 |
| BstMAI | GTCTC | 2 cut(s) | 293, 1977 |
| BstMCI | CGRYCG | 2 cut(s) | 91, 662 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 23, 32, 670, 1031, 1397, 1560 |
| BstNI | CCWGG | 5 cut(s) | 161, 366, 1836, 1913, 2100 |
| BstNSI | RCATGY | 3 cut(s) | 668, 1088, 1663 |
| BstSCI | CCNGG | 5 cut(s) | 159, 364, 1834, 1911, 2098 |
| BstSFI | CTRYAG | 3 cut(s) | 1197, 1271, 1344 |
| BstUI | CGCG | 2 cut(s) | 87, 93 |
| BstV1I | GCAGC | 2 cut(s) | 1183, 1818 |
| BstV2I | GAAGAC | 1 cut(s) | 1109 |
| BstX2I | RGATCY | 4 cut(s) | 523, 585, 748, 1708 |
| BstYI | RGATCY | 4 cut(s) | 523, 585, 748, 1708 |
| BstZI | CGGCCG | 1 cut(s) | 88 |
| Bsu36I | CCTNAGG | 1 cut(s) | 554 |
| BsuI | GTATCC | 3 cut(s) | 130, 162, 985 |
| BtsCI | GGATG | 7 cut(s) | 244, 497, 811, 1053, 1904, 2010, 2071 |
| BtsI | GCAGTG | 2 cut(s) | 675, 766 |
| BtsIMutI | CAGTG | 8 cut(s) | 675, 766, 1002, 1071, 1249, 1317, 1649, 2023 |
| BveI | ACCTGC | 1 cut(s) | 452 |
| Cac8I | GCNNGC | 6 cut(s) | 65, 550, 666, 757, 2035, 2039 |
| CciI | TCATGA | 1 cut(s) | 1975 |
| CciNI | GCGGCCGC | 1 cut(s) | 88 |
| CfoI | GCGC | 3 cut(s) | 87, 1554, 1956 |
| Cfr10I | RCCGGY | 2 cut(s) | 755, 1183 |
| Cfr13I | GGNCC | 1 cut(s) | 1746 |
| Csp6I | GTAC | 3 cut(s) | 228, 709, 893 |
| CspAI | ACCGGT | 1 cut(s) | 1183 |
| CviAII | CATG | 9 cut(s) | 209, 284, 373, 665, 1085, 1235, 1660, 1840, 1976 |
| CviQI | GTAC | 3 cut(s) | 228, 709, 893 |
| DdeI | CTNAG | 9 cut(s) | 380, 515, 554, 838, 938, 951, 1172, 1559, 1593 |
| DraIII | CACNNNGTG | 1 cut(s) | 1006 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1409 |
| EaeI | YGGCCR | 4 cut(s) | 24, 88, 502, 1132 |
| EagI | CGGCCG | 1 cut(s) | 88 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1409 |
| Ecl136II | GAGCTC | 2 cut(s) | 17, 423 |
| EclXI | CGGCCG | 1 cut(s) | 88 |
| Eco130I | CCWWGG | 1 cut(s) | 183 |
| Eco147I | AGGCCT | 1 cut(s) | 2041 |
| Eco24I | GRGCYC | 3 cut(s) | 19, 372, 425 |
| Eco31I | GGTCTC | 1 cut(s) | 293 |
| Eco32I | GATATC | 1 cut(s) | 1493 |
| Eco47III | AGCGCT | 1 cut(s) | 1955 |
| Eco52I | CGGCCG | 1 cut(s) | 88 |
| Eco53kI | GAGCTC | 2 cut(s) | 17, 423 |
| Eco57I | CTGAAG | 1 cut(s) | 1103 |
| Eco81I | CCTNAGG | 1 cut(s) | 554 |
| EcoICRI | GAGCTC | 2 cut(s) | 17, 423 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1274 |
| EcoRII | CCWGG | 5 cut(s) | 159, 364, 1834, 1911, 2098 |
| EcoRV | GATATC | 1 cut(s) | 1493 |
| EcoT14I | CCWWGG | 1 cut(s) | 183 |
| EcoT22I | ATGCAT | 2 cut(s) | 214, 257 |
| EcoT38I | GRGCYC | 3 cut(s) | 19, 372, 425 |
| ErhI | CCWWGG | 1 cut(s) | 183 |
| FaeI | CATG | 9 cut(s) | 212, 287, 376, 668, 1088, 1238, 1663, 1843, 1979 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 185, 217 |
| FaqI | GGGAC | 3 cut(s) | 364, 1679, 1929 |
| FatI | CATG | 9 cut(s) | 208, 283, 372, 664, 1084, 1234, 1659, 1839, 1975 |
| FauNDI | CATATG | 2 cut(s) | 310, 1506 |
| FbaI | TGATCA | 1 cut(s) | 205 |
| FblI | GTMKAC | 1 cut(s) | 243 |
| Fnu4HI | GCNGC | 5 cut(s) | 24, 88, 91, 1197, 1807 |
| FokI | GGATG | 7 cut(s) | 251, 484, 818, 1040, 1891, 2017, 2058 |
| FriOI | GRGCYC | 3 cut(s) | 19, 372, 425 |
| Fsp4HI | GCNGC | 5 cut(s) | 24, 88, 91, 1197, 1807 |
| FspBI | CTAG | 4 cut(s) | 1151, 1857, 2043, 2106 |
| FspI | TGCGCA | 1 cut(s) | 1553 |
| GlaI | GCGC | 3 cut(s) | 86, 1553, 1955 |
| GluI | GCNGC | 5 cut(s) | 24, 88, 91, 1197, 1807 |
| GsuI | CTGGAG | 1 cut(s) | 182 |
| HaeII | RGCGCY | 1 cut(s) | 1957 |
| HapII | CCGG | 6 cut(s) | 20, 321, 501, 712, 756, 1184 |
| HhaI | GCGC | 3 cut(s) | 87, 1554, 1956 |
| Hin1II | CATG | 9 cut(s) | 212, 287, 376, 668, 1088, 1238, 1663, 1843, 1979 |
| Hin6I | GCGC | 3 cut(s) | 85, 1552, 1954 |
| HinP1I | GCGC | 3 cut(s) | 85, 1552, 1954 |
| HincII | GTYRAC | 1 cut(s) | 985 |
| HindII | GTYRAC | 1 cut(s) | 985 |
| HinfI | GANTC | 5 cut(s) | 175, 537, 715, 1163, 1728 |
| HpaII | CCGG | 6 cut(s) | 20, 321, 501, 712, 756, 1184 |
| HphI | GGTGA | 1 cut(s) | 1779 |
| Hpy166II | GTNNAC | 4 cut(s) | 244, 764, 827, 985 |
| Hpy188I | TCNGA | 8 cut(s) | 405, 427, 518, 775, 939, 954, 1093, 1924 |
| Hpy8I | GTNNAC | 4 cut(s) | 244, 764, 827, 985 |
| Hpy99I | CGWCG | 1 cut(s) | 621 |
| HpyAV | CCTTC | 5 cut(s) | 871, 1186, 1270, 1969, 1998 |
| HpyCH4IV | ACGT | 1 cut(s) | 766 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 23, 32, 670, 1031, 1397, 1560 |
| HpyF3I | CTNAG | 9 cut(s) | 380, 515, 554, 838, 938, 951, 1172, 1559, 1593 |
| HpySE526I | ACGT | 1 cut(s) | 766 |
| Hsp92II | CATG | 9 cut(s) | 212, 287, 376, 668, 1088, 1238, 1663, 1843, 1979 |
| HspAI | GCGC | 3 cut(s) | 85, 1552, 1954 |
| KroI | GCCGGC | 1 cut(s) | 755 |
| KroNI | GCCGGC | 1 cut(s) | 757 |
| Ksp22I | TGATCA | 1 cut(s) | 205 |
| LmnI | GCTCC | 5 cut(s) | 22, 452, 1780, 1951, 1961 |
| Lsp1109I | GCAGC | 2 cut(s) | 1183, 1818 |
| LweI | GCATC | 2 cut(s) | 1289, 1330 |
| MaeI | CTAG | 4 cut(s) | 1151, 1857, 2043, 2106 |
| MaeII | ACGT | 1 cut(s) | 766 |
| MaeIII | GTNAC | 5 cut(s) | 439, 1310, 1526, 1785, 1799 |
| MboII | GAAGA | 4 cut(s) | 202, 645, 1109, 1751 |
| MflI | RGATCY | 4 cut(s) | 523, 585, 748, 1708 |
| MhlI | GDGCHC | 4 cut(s) | 19, 372, 425, 2025 |
| MluCI | AATT | 7 cut(s) | 232, 431, 684, 911, 946, 1453, 1601 |
| MmeI | TCCRAC | 1 cut(s) | 1525 |
| Mph1103I | ATGCAT | 2 cut(s) | 214, 257 |
| MroNI | GCCGGC | 1 cut(s) | 755 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1251 |
| MseI | TTAA | 9 cut(s) | 102, 599, 687, 800, 819, 1394, 1632, 1719, 1967 |
| MspA1I | CMGCKG | 1 cut(s) | 578 |
| MspI | CCGG | 6 cut(s) | 20, 321, 501, 712, 756, 1184 |
| MspR9I | CCNGG | 5 cut(s) | 161, 366, 1836, 1913, 2100 |
| Mva1269I | GAATGC | 1 cut(s) | 1554 |
| MvaI | CCWGG | 5 cut(s) | 161, 366, 1836, 1913, 2100 |
| MvnI | CGCG | 2 cut(s) | 87, 93 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 23, 32, 670, 1031, 1397, 1560 |
| NaeI | GCCGGC | 1 cut(s) | 757 |
| NdeI | CATATG | 2 cut(s) | 310, 1506 |
| NgoMIV | GCCGGC | 1 cut(s) | 755 |
| NlaIII | CATG | 9 cut(s) | 212, 287, 376, 668, 1088, 1238, 1663, 1843, 1979 |
| NlaIV | GGNNCC | 3 cut(s) | 525, 811, 1747 |
| NmeAIII | GCCGAG | 1 cut(s) | 30 |
| NmuCI | GTSAC | 1 cut(s) | 1785 |
| NotI | GCGGCCGC | 1 cut(s) | 88 |
| NsbI | TGCGCA | 1 cut(s) | 1553 |
| NsiI | ATGCAT | 2 cut(s) | 214, 257 |
| NspI | RCATGY | 3 cut(s) | 668, 1088, 1663 |
| PaeI | GCATGC | 1 cut(s) | 668 |
| PagI | TCATGA | 1 cut(s) | 1975 |
| PasI | CCCWGGG | 1 cut(s) | 1912 |
| PceI | AGGCCT | 1 cut(s) | 2041 |
| PciI | ACATGT | 2 cut(s) | 1084, 1659 |
| PctI | GAATGC | 1 cut(s) | 1554 |
| PdiI | GCCGGC | 1 cut(s) | 757 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1251 |
| PfeI | GAWTC | 5 cut(s) | 175, 537, 715, 1163, 1728 |
| PinAI | ACCGGT | 1 cut(s) | 1183 |
| PkrI | GCNGC | 5 cut(s) | 25, 89, 92, 1198, 1808 |
| Ple19I | CGATCG | 1 cut(s) | 662 |
| PscI | ACATGT | 2 cut(s) | 1084, 1659 |
| Psp124BI | GAGCTC | 2 cut(s) | 19, 425 |
| Psp6I | CCWGG | 5 cut(s) | 159, 364, 1834, 1911, 2098 |
| PspGI | CCWGG | 5 cut(s) | 159, 364, 1834, 1911, 2098 |
| PspN4I | GGNNCC | 3 cut(s) | 525, 811, 1747 |
| PspPI | GGNCC | 1 cut(s) | 1746 |
| PstI | CTGCAG | 1 cut(s) | 1201 |
| PsuI | RGATCY | 4 cut(s) | 523, 585, 748, 1708 |
| PvuI | CGATCG | 1 cut(s) | 662 |
| PvuII | CAGCTG | 1 cut(s) | 578 |
| RsaI | GTAC | 3 cut(s) | 229, 710, 894 |
| RsaNI | GTAC | 3 cut(s) | 228, 709, 893 |
| SacI | GAGCTC | 2 cut(s) | 19, 425 |
| SaqAI | TTAA | 9 cut(s) | 102, 599, 687, 800, 819, 1394, 1632, 1719, 1967 |
| SatI | GCNGC | 5 cut(s) | 24, 88, 91, 1197, 1807 |
| Sau96I | GGNCC | 1 cut(s) | 1746 |
| ScrFI | CCNGG | 5 cut(s) | 161, 366, 1836, 1913, 2100 |
| SduI | GDGCHC | 4 cut(s) | 19, 372, 425, 2025 |
| SfaNI | GCATC | 2 cut(s) | 1289, 1330 |
| SfcI | CTRYAG | 3 cut(s) | 1197, 1271, 1344 |
| SmlI | CTYRAG | 3 cut(s) | 1292, 1884, 2076 |
| SmoI | CTYRAG | 3 cut(s) | 1292, 1884, 2076 |
| SphI | GCATGC | 1 cut(s) | 668 |
| Sse9I | AATT | 7 cut(s) | 232, 431, 684, 911, 946, 1453, 1601 |
| SseBI | AGGCCT | 1 cut(s) | 2041 |
| SsiI | CCGC | 4 cut(s) | 23, 87, 91, 1315 |
| SspI | AATATT | 3 cut(s) | 396, 817, 1225 |
| SspMI | CTAG | 4 cut(s) | 1151, 1857, 2043, 2106 |
| SstI | GAGCTC | 2 cut(s) | 19, 425 |
| StuI | AGGCCT | 1 cut(s) | 2041 |
| StyD4I | CCNGG | 5 cut(s) | 159, 364, 1834, 1911, 2098 |
| StyI | CCWWGG | 1 cut(s) | 183 |
| TaiI | ACGT | 1 cut(s) | 769 |
| TaqI | TCGA | 6 cut(s) | 178, 658, 1030, 1470, 1898, 2064 |
| TaqII | GACCGA | 1 cut(s) | 1923 |
| TasI | AATT | 7 cut(s) | 232, 431, 684, 911, 946, 1453, 1601 |
| TatI | WGTACW | 1 cut(s) | 227 |
| TauI | GCSGC | 3 cut(s) | 26, 90, 93 |
| TfiI | GAWTC | 5 cut(s) | 175, 537, 715, 1163, 1728 |
| Tru1I | TTAA | 9 cut(s) | 102, 599, 687, 800, 819, 1394, 1632, 1719, 1967 |
| Tru9I | TTAA | 9 cut(s) | 102, 599, 687, 800, 819, 1394, 1632, 1719, 1967 |
| TscAI | CASTG | 8 cut(s) | 675, 766, 1009, 1078, 1249, 1324, 1656, 2030 |
| TseFI | GTSAC | 1 cut(s) | 1785 |
| TseI | GCWGC | 2 cut(s) | 1196, 1806 |
| Tsp45I | GTSAC | 1 cut(s) | 1785 |
| TspDTI | ATGAA | 6 cut(s) | 272, 444, 1251, 1455, 1707, 2037 |
| TspGWI | ACGGA | 4 cut(s) | 253, 634, 650, 1478 |
| TspRI | CASTG | 8 cut(s) | 675, 766, 1009, 1078, 1249, 1324, 1656, 2030 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1274 |
| XapI | RAATTY | 1 cut(s) | 431 |
| XbaI | TCTAGA | 1 cut(s) | 1856 |
| XceI | RCATGY | 3 cut(s) | 668, 1088, 1663 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1064 |
| XmiI | GTMKAC | 1 cut(s) | 243 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1251 |
| XspI | CTAG | 4 cut(s) | 1151, 1857, 2043, 2106 |
| Zsp2I | ATGCAT | 2 cut(s) | 214, 257 |