FvH4_2g20301

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000654
Physical Location & Seq
Reverse (-)
17072197 .. 17073036
840 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20301.t1

Sequence Viewer

Length: 480 bp
ATGGTGTCGAAATGGAGAAGAGCTCTTAAAGATGTCGCGGATCTTGGTGGAATGGTTTTAGGAGATCACCATTGGCCATTTGGGACAGATGATCAGCAGGGTGGGGAAGAATTGTGTGTTTCTGTGGATCAGGGGTATGAAAACTTGATAAAGGATTTCGGTGTCCAGCTTGTGTATGAGCAAGAAAATAGGGGCAATACTTGTGAAGGTGTCCAGTCAAACGGGGAAGATGTTACAGTTCACCAGCAAGTATCTTACTGGAGTCAGGATCATGTTGTTGGAGACGTGTCAGTGTCAGCATCGATGTATCAAATGTGGAGAGGCAAGTACTTTTTGTGTAATCTTAGGTTTGATGCCTATCAGAATCAATTCAAATGGAGCCTGAGGAATCCAGCTCTTCTTGATTTTTCATATGAGCCAGATGGTCCATACAATTTTTTATGTAAGCACCAAGCTGCAGAAGATTGTGAGGAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

160

Amino Acids

18.26

Weight (kDa)

4.56

Isoelectric Point (pI)

23.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 38
AciI CCGC 1 cut(s) 38
AclWI GGATC 3 cut(s) 48, 135, 276
AcoI YGGCCR 1 cut(s) 74
AfaI GTAC 1 cut(s) 329
AflIII ACRYGT 1 cut(s) 285
AgsI TTSAA 1 cut(s) 373
AjiI CACGTC 1 cut(s) 286
AluBI AGCT 4 cut(s) 23, 169, 395, 455
AluI AGCT 4 cut(s) 23, 169, 395, 455
Alw21I GWGCWC 1 cut(s) 25
Alw26I GTCTC 1 cut(s) 276
AlwI GGATC 3 cut(s) 48, 135, 276
AoxI GGCC 1 cut(s) 74
ApeKI GCWGC 1 cut(s) 455
Asp700I GAANNNNTTC 1 cut(s) 368
AspS9I GGNCC 1 cut(s) 425
AsuHPI GGTGA 2 cut(s) 59, 233
AvaII GGWCC 1 cut(s) 425
AxyI CCTNAGG 1 cut(s) 383
BalI TGGCCA 1 cut(s) 76
BanII GRGCYC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 25
BbvI GCAGC 1 cut(s) 442
BccI CCATC 1 cut(s) 416
BclI TGATCA 1 cut(s) 91
BcoDI GTCTC 1 cut(s) 276
BfmI CTRYAG 1 cut(s) 456
BisI GCNGC 1 cut(s) 456
BlsI GCNGC 1 cut(s) 457
BmcAI AGTACT 1 cut(s) 329
Bme18I GGWCC 1 cut(s) 425
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 425
BmiI GGNNCC 1 cut(s) 380
BmsI GCATC 2 cut(s) 308, 343
BplI GAGNNNNNCTC 2 cut(s) 7, 39
BpmI CTGGAG 1 cut(s) 280
Bsa29I ATCGAT 1 cut(s) 302
BsaBI GATNNNNATC 1 cut(s) 357
Bse1I ACTGG 2 cut(s) 214, 263
Bse21I CCTNAGG 1 cut(s) 383
Bse8I GATNNNNATC 1 cut(s) 357
BseCI ATCGAT 1 cut(s) 302
BseJI GATNNNNATC 1 cut(s) 357
BseMII CTCAG 1 cut(s) 374
BseNI ACTGG 2 cut(s) 214, 263
BseXI GCAGC 1 cut(s) 442
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 1 cut(s) 76
BshVI ATCGAT 1 cut(s) 302
BsiHKAI GWGCWC 1 cut(s) 25
BslFI GGGAC 1 cut(s) 97
BsmAI GTCTC 1 cut(s) 276
BsmBI CGTCTC 1 cut(s) 276
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 25
Bsp143I GATC 5 cut(s) 40, 64, 91, 127, 268
BspACI CCGC 1 cut(s) 38
BspANI GGCC 1 cut(s) 76
BspCNI CTCAG 1 cut(s) 375
BspDI ATCGAT 1 cut(s) 302
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 1 cut(s) 380
BspMAI CTGCAG 1 cut(s) 460
BspPI GGATC 3 cut(s) 48, 135, 276
BspQI GCTCTTC 2 cut(s) 13, 402
BsrI ACTGG 2 cut(s) 214, 263
BssMI GATC 5 cut(s) 40, 64, 91, 127, 268
Bst4CI ACNGT 1 cut(s) 238
Bst6I CTCTTC 2 cut(s) 13, 402
BstDEI CTNAG 2 cut(s) 344, 383
BstFNI CGCG 1 cut(s) 38
BstKTI GATC 5 cut(s) 43, 67, 94, 130, 271
BstMAI GTCTC 1 cut(s) 276
BstMBI GATC 5 cut(s) 40, 64, 91, 127, 268
BstSFI CTRYAG 1 cut(s) 456
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 1 cut(s) 442
BstX2I RGATCY 1 cut(s) 40
BstYI RGATCY 1 cut(s) 40
Bsu15I ATCGAT 1 cut(s) 302
Bsu36I CCTNAGG 1 cut(s) 383
BsuRI GGCC 1 cut(s) 76
BsuTUI ATCGAT 1 cut(s) 302
BtrI CACGTC 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 297
Cfr13I GGNCC 1 cut(s) 425
ClaI ATCGAT 1 cut(s) 302
Csp6I GTAC 1 cut(s) 328
CviAII CATG 1 cut(s) 272
CviJI RGCY 7 cut(s) 23, 76, 169, 381, 395, 418, 455
CviKI_1 RGCY 7 cut(s) 23, 76, 169, 381, 395, 418, 455
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 2 cut(s) 344, 383
DpnI GATC 5 cut(s) 42, 66, 93, 129, 270
DpnII GATC 5 cut(s) 40, 64, 91, 127, 268
EaeI YGGCCR 1 cut(s) 74
Eam1104I CTCTTC 2 cut(s) 13, 402
EarI CTCTTC 2 cut(s) 13, 402
Ecl136II GAGCTC 1 cut(s) 23
Eco24I GRGCYC 1 cut(s) 25
Eco47I GGWCC 1 cut(s) 425
Eco53kI GAGCTC 1 cut(s) 23
Eco81I CCTNAGG 1 cut(s) 383
EcoICRI GAGCTC 1 cut(s) 23
EcoT38I GRGCYC 1 cut(s) 25
Esp3I CGTCTC 1 cut(s) 276
FaeI CATG 1 cut(s) 275
FaiI YATR 7 cut(s) 138, 177, 273, 412, 414, 430, 442
FaqI GGGAC 1 cut(s) 97
FatI CATG 1 cut(s) 271
FauNDI CATATG 1 cut(s) 412
FbaI TGATCA 1 cut(s) 91
Fnu4HI GCNGC 1 cut(s) 456
FriOI GRGCYC 1 cut(s) 25
Fsp4HI GCNGC 1 cut(s) 456
GluI GCNGC 1 cut(s) 456
GsuI CTGGAG 1 cut(s) 280
HaeIII GGCC 1 cut(s) 76
Hin1II CATG 1 cut(s) 275
HinfI GANTC 3 cut(s) 262, 364, 388
HphI GGTGA 2 cut(s) 59, 233
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 1 cut(s) 363
Hpy188III TCNNGA 2 cut(s) 266, 401
Hpy8I GTNNAC 1 cut(s) 241
HpyAV CCTTC 1 cut(s) 200
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 1 cut(s) 285
HpyCH4V TGCA 1 cut(s) 458
HpyF3I CTNAG 2 cut(s) 344, 383
HpySE526I ACGT 1 cut(s) 285
Hsp92II CATG 1 cut(s) 275
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 5 cut(s) 40, 64, 91, 127, 268
LguI GCTCTTC 2 cut(s) 13, 402
LmnI GCTCC 1 cut(s) 378
Lsp1109I GCAGC 1 cut(s) 442
LweI GCATC 2 cut(s) 308, 343
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 232
MalI GATC 5 cut(s) 42, 66, 93, 129, 270
MboI GATC 5 cut(s) 40, 64, 91, 127, 268
MboII GAAGA 5 cut(s) 30, 119, 239, 389, 473
MflI RGATCY 1 cut(s) 40
MhlI GDGCHC 1 cut(s) 25
MlsI TGGCCA 1 cut(s) 76
MluCI AATT 4 cut(s) 110, 368, 433, 475
MluNI TGGCCA 1 cut(s) 76
MlyI GAGTC 1 cut(s) 271
MmeI TCCRAC 1 cut(s) 259
MnlI CCTC 3 cut(s) 314, 378, 463
Mox20I TGGCCA 1 cut(s) 76
MroXI GAANNNNTTC 1 cut(s) 368
MscI TGGCCA 1 cut(s) 76
MseI TTAA 2 cut(s) 27, 478
Msp20I TGGCCA 1 cut(s) 76
MvnI CGCG 1 cut(s) 38
NdeI CATATG 1 cut(s) 412
NdeII GATC 5 cut(s) 40, 64, 91, 127, 268
NlaIII CATG 1 cut(s) 275
NlaIV GGNNCC 1 cut(s) 380
PciSI GCTCTTC 2 cut(s) 13, 402
PdmI GAANNNNTTC 1 cut(s) 368
PfeI GAWTC 2 cut(s) 364, 388
PkrI GCNGC 1 cut(s) 457
PleI GAGTC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 270
Psp124BI GAGCTC 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 380
PspPI GGNCC 1 cut(s) 425
PstI CTGCAG 1 cut(s) 460
PsuI RGATCY 1 cut(s) 40
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
SacI GAGCTC 1 cut(s) 25
SapI GCTCTTC 2 cut(s) 13, 402
SaqAI TTAA 2 cut(s) 27, 478
SatI GCNGC 1 cut(s) 456
Sau3AI GATC 5 cut(s) 40, 64, 91, 127, 268
Sau96I GGNCC 1 cut(s) 425
ScaI AGTACT 1 cut(s) 329
SchI GAGTC 1 cut(s) 271
SduI GDGCHC 1 cut(s) 25
SetI ASST 7 cut(s) 25, 171, 211, 288, 350, 397, 457
SfaNI GCATC 2 cut(s) 308, 343
SfcI CTRYAG 1 cut(s) 456
SinI GGWCC 1 cut(s) 425
Sse9I AATT 4 cut(s) 110, 368, 433, 475
SsiI CCGC 1 cut(s) 38
SstI GAGCTC 1 cut(s) 25
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 1 cut(s) 288
TaqI TCGA 2 cut(s) 8, 302
TasI AATT 4 cut(s) 110, 368, 433, 475
TatI WGTACW 1 cut(s) 327
TfiI GAWTC 2 cut(s) 364, 388
Tru1I TTAA 2 cut(s) 27, 478
Tru9I TTAA 2 cut(s) 27, 478
TscAI CASTG 1 cut(s) 297
TseI GCWGC 1 cut(s) 455
TspDTI ATGAA 2 cut(s) 153, 399
TspRI CASTG 1 cut(s) 297
VpaK11BI GGWCC 1 cut(s) 425
XcmI CCANNNNNNNNNTGG 1 cut(s) 77
XmnI GAANNNNTTC 1 cut(s) 368
ZrmI AGTACT 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.