pycom15g33580

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000654
Physical Location & Seq
Forward (+)
33064603 .. 33068624
4022 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g33580.2

Sequence Viewer

Length: 3492 bp
ATGCGCACCGAGCAACAGAATGCTTCTTCGTCCTCTTACCGGTGCACGTATGATGCATTCTTGAGTTTCAGAGGCACAGATACACGCAAGGGCTTTACAGATCACCTCTACAGTGCTTTGGAGTGGGCAGGAATCCACACTTTTAGGGACGATGAAGAAATTGAGACAGGAGAAAACATTGAACAGGAGCTACGGACGGCAATACAAGAGTCACGAGTATCAATCATTGTTTTCTCCAAGGACTACGCCTCTTCCAGTTGGTGCCTGAATGAACTTGCTATCATCATGGAACGTAAAAGAACAGATGGACACATGGTTATCCCAGTTTTCTATGATGTGGAACCATCAGATGTCAGGAAGCAGACGGGCAGTTTTGCAGAATCATTTACTAAACATGAAAAGCGCTTCAAGGACAAGGTGGAGGAGTGGAAGCGAGCTCTTAGAGATGTTGCAGATTTGGCAGGGATGGTTTATAGAGAACAGTACGAGTCGCAGTTTATCCAAGATATTGTTGAAGCGATTGGAAATAAGCTGAACGACATGACGAATACGAGATTAAGAGTTGAGCCCAATGTGATTGGAAGAGATTATCATGTGAAAAGACTAAACATGTGGCTGGAAGATGGATCAAATGATGTTGGAGTATCTGTCATCTATGGAATGGGTGGAATAGGCAAGACCACCATTGCTAAAACTGTTTATAACCAGAACTCCGATAAATTTCAAGCTAGCAGCTTTCTTTCAGATATTTGGGAAACTTCCAAACAAGGCAATGGTATTGTTCACTTGCAAAGGAAGCTTCTTTCAGATATCCAAAAGGGGAAAATGGAAAAAATATACAGCCTTGATGAAGGTATGGTAAAGATCGAACGGGTTGTACGTTGCAAAAGAGTTCTTATTGTTCTTGATGATGTGGAGAACTCAGAACAGTTCAATGCAATTCTTGGAATGCAAGACTGGTTCCATCCTGGAAGTAAAATTATCATAACAACTAGACATGAACATTTGTTAAAGGCTCATGAAGTTTGTGTAAGGTTTAAGGTTGAAGGATTACATGCGCATCAATCTCTTATGCTTTTCAGTTGGCATGCCTTCGGACAACCCCATCCTCAGGAAGGTTACATGGAGCATTCAAGAACCTTAGTAAAACATTGTGGAGGGGTTCCATTAGCTCTTCAAGTTCTGGGATCTTCTCTATTTGAAAAAACAGCAAATGTATGGGAAAATGTAGTACGCAACTTAGATGTTTTAGACGTGATTACTGAGGGAAAAATTAAAAAGATTCTTTGCAAAAGTTATGAATCTTTACAAGATCATGATAGACGTTTATTCCTCCATATAGCCTGTTTCTTCGTAGGAAAGGACAAGGATTTCGCAACTACAATCCTTGACAAATGTGATTTTGTAACAGAGATTGGACTTCAACATCTAGTTGATAGATGCCTTTTGGAAATTGATGCCCACAGCAAGTTAACCATGCATCAATTGCTTCAAGACATGGGAAGGGCAATAATTCGCGACGAATCACCTGAGGACCCTGGAAAACGCACTAGACTGTGGGATAAAGATGCAATTAACGTTTTGAGAAAATTAACTGGCACGAGAACTATCAAGGGCCTCGTGCTCAACTTTCCCTCAATGGAGTCATCTCCGGTATTAAATGAGGTAGGTTTCGAAACAAAGGCATTTACAGAGATGCTCAATCTTGAACTACTTCTGCTTGATAATGTAAAGTTGAGTGGAAGCTATGAAGATTTTCCAAAAAATTTAATATGGCTGTCTTGGCGAGGATTCTCTTTAAAATCAATACCAGCCAATTTTTGTATGGAAAATCTAGTTGTTCTTGACTTACGGAAGAGCAGTCTGCAACATGTTTGGAACGGAACCAAGCTTCTTACAAGATTGAAAATTCTTAATCTCAGTCATTCACATGGCCTTAGGACAACGTCTGACTTGTCCGGACTTCCTAACCTTGAGAAACTAATTCTTAAGGACTGCATTAATTTGGTTGATGTTGATGAATCCATTGGAAACTTGGGGAAACTTGTTTTCTTGAATCTAAAAGACTGCAAAAGTCTTATGAAGCTTCCAAAAAGAATGAACATGATGAGGTCTCTTGAGGAACTTATTCTTACTGGTTGCTCAAAGCTTGTGCTTCATGACAGTGCCATGGTTATTTACATACACACTACATCAAGGGATATGAATATTTTTAGACTGTTGTCAACTATATCATGGACACCAATCTGGTCTTGGGGGATGTGGGCATGGCCAAGAAAGACTCTTCAATCAACCAGTTTCTCACTGGCAAGTTTACCACGTTGTTTGGGAAGCTTAAGTCTGTCTTACTGCAACGTGTCGGAGGTTCCCAATGATCTGTCTACACTATCTTCATTGAAGTATTTGAATCTAATTGGTAATCCAATTTTGTGCCTACCACAAAAAATGAAGAGTCTTATTATGCTCGAGACTCTTTTGTTAGATAATTGCACAAACCTCGAAATTCTTCCAGAGCTCCCACCAAGCTTGAAGAGGTTAGAAGCAAAATATTGTACTTCATTGAAAAGATTAACAAATTTACCAAACTTGTTCAGATCATTGGAATCACTTTTTTGGGGTTGTGAGCATTTAGTTGAAGTTGATAGCTTGTTGAATATAAAACCGGTGAGAAGCGCTGATGTAGAAATGACAAGATATATGGGCCTGTTCAATTTGAAATCCATAGCAAGCACTGATGTCGAAATGATCAACTACTTGACCAGTACAACCAGGAAGGCTCCTGTCCAGGTACTCTATGAATGTGGCATATATAGCATTTTCTTTCATGGAAGCAAGATTCCAGATGGATTTGGCTTCACAATAATATATAGATCTGTGCTGTCAGTTATTGTACCTTCACATCCTATTCTCAAAATCCGAGGCTTGAATTCATGTGTGTTATATGCACAAGAACCAGGCTTGGAATATCGTCTTACTGTTCAGCATTTGATTAAAGTCAGTAATCAGACCAAGGGTCTTATGTGGACCTATTCCCCACTCACAGTAGGTTTGCCTACTGAGAAGGAAGATATGTTATGGCTAAGCCATTTTAGATTTGGAAACAACGAATTGGAGTATGGGGACGAATTACGTGTTTCGGTGGAGATGCATGATTTTGTCCGGGTAAAGGAATTCGGAATCCAGCTTGTGTACGAGCCGGAAAGACCTCCTTCTGGCCAGAATAATGTTGTTACTGGAGATGTGTCACTATCAGCATCAGAGTACAAAATGTGGACAGGAAAATACTTTCTCAGTAATCATGGGCACCGTACTCATCAAGCTCAATTCCGAAAGAGACAGGAGAATCCAGAACATATTGAATTTTCATTTGGGCCAGAGCGTCTATTTCATAAATTATTCAAGGCCCGAGGACCATTCGAAACTGTTGTGAAACTTAGAGCATATCCAGCAGGTTCCGCATCTGCTTCTCCTCACCCATTTTGGCTAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1164

Amino Acids

133.09

Weight (kDa)

7.77

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 702
Acc16I TGCGCA 2 cut(s) 5, 1059
Acc36I ACCTGC 1 cut(s) 3440
AccB1I GGYRCC 2 cut(s) 261, 3303
AccI GTMKAC 1 cut(s) 2381
AccII CGCG 1 cut(s) 1518
AccIII TCCGGA 1 cut(s) 1958
AciI CCGC 1 cut(s) 3456
AclI AACGTT 1 cut(s) 1578
AclWI GGATC 2 cut(s) 634, 1195
AcoI YGGCCR 2 cut(s) 2270, 3214
AcsI RAATTY 8 cut(s) 719, 1765, 1908, 2502, 2575, 2926, 3170, 3359
AfeI AGCGCT 2 cut(s) 404, 2674
AfiI CCNNNNNNNGG 5 cut(s) 39, 1111, 1115, 3166, 3212
AflII CTTAAG 2 cut(s) 1988, 2335
AflIII ACRYGT 4 cut(s) 609, 1870, 2355, 3130
AgeI ACCGGT 2 cut(s) 39, 2662
AhdI GACNNNNNGTC 1 cut(s) 3012
AjiI CACGTC 1 cut(s) 1255
AjnI CCWGG 5 cut(s) 967, 1537, 2768, 2784, 2953
AjuI GAANNNNNNNTTGG 2 cut(s) 3351, 3383
Alw21I GWGCWC 4 cut(s) 47, 439, 1626, 2517
Alw26I GTCTC 4 cut(s) 158, 2118, 2462, 3328
Alw44I GTGCAC 1 cut(s) 43
AlwI GGATC 2 cut(s) 634, 1195
Ama87I CYCGRG 2 cut(s) 2465, 3405
Aor13HI TCCGGA 1 cut(s) 1958
Aor51HI AGCGCT 2 cut(s) 404, 2674
AoxI GGCC 7 cut(s) 1615, 1933, 2270, 2701, 3214, 3371, 3402
ApaLI GTGCAC 1 cut(s) 43
ApeKI GCWGC 1 cut(s) 732
ApoI RAATTY 8 cut(s) 719, 1765, 1908, 2502, 2575, 2926, 3170, 3359
ArsI GACNNNNNNTTYG 2 cut(s) 1355, 1387
AseI ATTAAT 1 cut(s) 2001
AsiGI ACCGGT 2 cut(s) 39, 2662
Asp700I GAANNNNTTC 4 cut(s) 1713, 1755, 2127, 2505
AspLEI GCGC 4 cut(s) 6, 405, 1060, 2675
AspS9I GGNCC 7 cut(s) 1534, 1615, 2701, 3024, 3371, 3403, 3410
AsuC2I CCSGG 1 cut(s) 3161
AsuHPI GGTGA 4 cut(s) 95, 1518, 2677, 3464
AsuII TTCGAA 2 cut(s) 1674, 3417
AsuNHI GCTAGC 1 cut(s) 728
AvaI CYCGRG 2 cut(s) 2465, 3405
AvaII GGWCC 3 cut(s) 1534, 3024, 3410
AxyI CCTNAGG 3 cut(s) 1110, 1530, 1937
BaeGI GKGCMC 2 cut(s) 47, 3306
BalI TGGCCA 2 cut(s) 2272, 3216
BanI GGYRCC 2 cut(s) 261, 3303
BanII GRGCYC 3 cut(s) 439, 570, 2517
BauI CACGAG 3 cut(s) 213, 1600, 1619
Bbv12I GWGCWC 4 cut(s) 47, 439, 1626, 2517
BbvI GCAGC 1 cut(s) 744
BccI CCATC 7 cut(s) 299, 352, 460, 617, 972, 1113, 2837
BceAI ACGGC 1 cut(s) 213
BcgI CGANNNNNNTGC 4 cut(s) 2479, 2513, 2719, 2753
BciT130I CCWGG 5 cut(s) 969, 1539, 2770, 2786, 2955
BclI TGATCA 1 cut(s) 2745
BcnI CCSGG 1 cut(s) 3161
BcoDI GTCTC 4 cut(s) 158, 2118, 2462, 3328
BfaI CTAG 5 cut(s) 729, 993, 1430, 1551, 1835
BfmI CTRYAG 1 cut(s) 109
BfoI RGCGCY 2 cut(s) 406, 2676
BfrI CTTAAG 2 cut(s) 1988, 2335
BfuAI ACCTGC 1 cut(s) 3440
BglII AGATCT 1 cut(s) 2870
BisI GCNGC 1 cut(s) 733
BlpI GCTNAGC 1 cut(s) 3080
BlsI GCNGC 1 cut(s) 734
Bme1390I CCNGG 6 cut(s) 969, 1539, 2770, 2786, 2955, 3161
Bme18I GGWCC 3 cut(s) 1534, 3024, 3410
BmeRI GACNNNNNGTC 1 cut(s) 3012
BmeT110I CYCGRG 2 cut(s) 2465, 3405
BmgBI CACGTC 1 cut(s) 1255
BmgT120I GGNCC 7 cut(s) 1534, 1615, 2701, 3024, 3371, 3403, 3410
BmrFI CCNGG 6 cut(s) 969, 1539, 2770, 2786, 2955, 3161
BmrI ACTGGG 1 cut(s) 317
BmtI GCTAGC 1 cut(s) 732
BmuI ACTGGG 1 cut(s) 317
BoxI GACNNNNGTC 1 cut(s) 2221
BpmI CTGGAG 1 cut(s) 3255
Bpu1102I GCTNAGC 1 cut(s) 3080
Bpu14I TTCGAA 2 cut(s) 1674, 3417
BpuEI CTTGAG 3 cut(s) 82, 1994, 2138
BpuMI CCSGG 1 cut(s) 3161
BsaAI YACGTR 2 cut(s) 48, 3131
BsaI GGTCTC 1 cut(s) 2118
BsaJI CCNNGG 6 cut(s) 237, 1537, 2169, 2917, 3009, 3406
BsaWI WCCGGW 4 cut(s) 39, 1651, 1958, 2662
BsaXI ACNNNNNCTCC 4 cut(s) 633, 663, 695, 725
Bsc4I CCNNNNNNNGG 5 cut(s) 39, 1111, 1115, 3166, 3212
Bse118I RCCGGY 2 cut(s) 39, 2662
Bse1I ACTGG 9 cut(s) 255, 323, 962, 1600, 2140, 2295, 2310, 2760, 3238
Bse21I CCTNAGG 3 cut(s) 1110, 1530, 1937
Bse3DI GCAATG 2 cut(s) 684, 778
BseAI TCCGGA 1 cut(s) 1958
BseBI CCWGG 5 cut(s) 969, 1539, 2770, 2786, 2955
BseDI CCNNGG 6 cut(s) 237, 1537, 2169, 2917, 3009, 3406
BseGI GGATG 5 cut(s) 471, 964, 1105, 2265, 2899
BseLI CCNNNNNNNGG 5 cut(s) 39, 1111, 1115, 3166, 3212
BseMI GCAATG 2 cut(s) 684, 778
BseMII CTCAG 7 cut(s) 936, 1124, 1254, 1521, 1933, 3048, 3304
BseNI ACTGG 9 cut(s) 255, 323, 962, 1600, 2140, 2295, 2310, 2760, 3238
BseRI GAGGAG 2 cut(s) 437, 3459
BseSI GKGCMC 2 cut(s) 47, 3306
BseXI GCAGC 1 cut(s) 744
Bsh1236I CGCG 1 cut(s) 1518
BshFI GGCC 7 cut(s) 1617, 1935, 2272, 2703, 3216, 3373, 3404
BshNI GGYRCC 2 cut(s) 261, 3303
BshTI ACCGGT 2 cut(s) 39, 2662
BsiHKAI GWGCWC 4 cut(s) 47, 439, 1626, 2517
BsiHKCI CYCGRG 2 cut(s) 2465, 3405
BsiSI CCGG 6 cut(s) 40, 1652, 1959, 2663, 3160, 3197
BslFI GGGAC 2 cut(s) 161, 3134
BslI CCNNNNNNNGG 5 cut(s) 39, 1111, 1115, 3166, 3212
BsmAI GTCTC 4 cut(s) 158, 2118, 2462, 3328
BsmFI GGGAC 2 cut(s) 161, 3134
BsmI GAATGC 4 cut(s) 25, 56, 954, 1129
BsnI GGCC 7 cut(s) 1617, 1935, 2272, 2703, 3216, 3373, 3404
Bso31I GGTCTC 1 cut(s) 2118
BsoBI CYCGRG 2 cut(s) 2465, 3405
Bsp119I TTCGAA 2 cut(s) 1674, 3417
Bsp1286I GDGCHC 6 cut(s) 47, 439, 570, 1626, 2517, 3306
Bsp13I TCCGGA 1 cut(s) 1958
Bsp143I GATC 9 cut(s) 100, 626, 864, 1187, 1312, 2374, 2594, 2745, 2870
Bsp1720I GCTNAGC 1 cut(s) 3080
Bsp19I CCATGG 1 cut(s) 2169
Bsp68I TCGCGA 1 cut(s) 1518
BspACI CCGC 1 cut(s) 3456
BspANI GGCC 7 cut(s) 1617, 1935, 2272, 2703, 3216, 3373, 3404
BspCNI CTCAG 7 cut(s) 935, 1123, 1255, 1522, 1932, 3049, 3303
BspEI TCCGGA 1 cut(s) 1958
BspFNI CGCG 1 cut(s) 1518
BspHI TCATGA 3 cut(s) 1018, 1315, 2158
BspMI ACCTGC 1 cut(s) 3440
BspOI GCTAGC 1 cut(s) 732
BspPI GGATC 2 cut(s) 634, 1195
BspQI GCTCTTC 2 cut(s) 1179, 1850
BspT104I TTCGAA 2 cut(s) 1674, 3417
BspT107I GGYRCC 2 cut(s) 261, 3303
BspTI CTTAAG 2 cut(s) 1988, 2335
BspTNI GGTCTC 1 cut(s) 2118
BsrDI GCAATG 2 cut(s) 684, 778
BsrFI RCCGGY 2 cut(s) 39, 2662
BsrI ACTGG 9 cut(s) 255, 323, 962, 1600, 2140, 2295, 2310, 2760, 3238
BssAI RCCGGY 2 cut(s) 39, 2662
BssECI CCNNGG 6 cut(s) 237, 1537, 2169, 2917, 3009, 3406
BssMI GATC 9 cut(s) 100, 626, 864, 1187, 1312, 2374, 2594, 2745, 2870
BssSI CACGAG 3 cut(s) 213, 1600, 1619
BssT1I CCWWGG 3 cut(s) 237, 2169, 3009
Bst2BI CACGAG 3 cut(s) 213, 1600, 1619
Bst2UI CCWGG 5 cut(s) 969, 1539, 2770, 2786, 2955
Bst6I CTCTTC 7 cut(s) 256, 577, 1179, 1850, 2289, 2444, 2525
BstAFI CTTAAG 2 cut(s) 1988, 2335
BstAPI GCANNNNNTGC 1 cut(s) 1486
BstBAI YACGTR 2 cut(s) 48, 3131
BstBI TTCGAA 2 cut(s) 1674, 3417
BstC8I GCNNGC 4 cut(s) 435, 730, 1089, 2728
BstDSI CCRYGG 1 cut(s) 2169
BstENI CCTNNNNNAGG 1 cut(s) 1113
BstF5I GGATG 5 cut(s) 471, 964, 1105, 2265, 2899
BstFNI CGCG 1 cut(s) 1518
BstH2I RGCGCY 2 cut(s) 406, 2676
BstHHI GCGC 4 cut(s) 6, 405, 1060, 2675
BstKTI GATC 9 cut(s) 103, 629, 867, 1190, 1315, 2377, 2597, 2748, 2873
BstMAI GTCTC 4 cut(s) 158, 2118, 2462, 3328
BstMBI GATC 9 cut(s) 100, 626, 864, 1187, 1312, 2374, 2594, 2745, 2870
BstMWI GCNNNNNNNGC 8 cut(s) 10, 458, 796, 1486, 1783, 2811, 3446, 3455
BstNI CCWGG 5 cut(s) 969, 1539, 2770, 2786, 2955
BstNSI RCATGY 4 cut(s) 613, 1058, 1091, 1874
BstPAI GACNNNNGTC 1 cut(s) 2221
BstSCI CCNGG 6 cut(s) 967, 1537, 2768, 2784, 2953, 3159
BstSFI CTRYAG 1 cut(s) 109
BstSLI GKGCMC 2 cut(s) 47, 3306
BstUI CGCG 1 cut(s) 1518
BstV1I GCAGC 1 cut(s) 744
BstX2I RGATCY 2 cut(s) 1187, 2870
BstYI RGATCY 2 cut(s) 1187, 2870
Bsu36I CCTNAGG 3 cut(s) 1110, 1530, 1937
BsuRI GGCC 7 cut(s) 1617, 1935, 2272, 2703, 3216, 3373, 3404
BtgI CCRYGG 1 cut(s) 2169
BtrI CACGTC 1 cut(s) 1255
BtsCI GGATG 5 cut(s) 471, 964, 1105, 2265, 2899
BtsIMutI CAGTG 4 cut(s) 118, 2170, 2303, 2730
BtuMI TCGCGA 1 cut(s) 1518
BveI ACCTGC 1 cut(s) 3440
Cac8I GCNNGC 4 cut(s) 435, 730, 1089, 2728
CciI TCATGA 3 cut(s) 1018, 1315, 2158
CfoI GCGC 4 cut(s) 6, 405, 1060, 2675
Cfr10I RCCGGY 2 cut(s) 39, 2662
Cfr13I GGNCC 7 cut(s) 1534, 1615, 2701, 3024, 3371, 3403, 3410
CseI GACGC 1 cut(s) 3368
CspAI ACCGGT 2 cut(s) 39, 2662
CspCI CAANNNNNGTGG 4 cut(s) 2307, 2342, 2508, 2543
DpnI GATC 9 cut(s) 102, 628, 866, 1189, 1314, 2376, 2596, 2747, 2872
DpnII GATC 9 cut(s) 100, 626, 864, 1187, 1312, 2374, 2594, 2745, 2870
DraI TTTAAA 1 cut(s) 1800
DriI GACNNNNNGTC 1 cut(s) 3012
EaeI YGGCCR 2 cut(s) 2270, 3214
Eam1104I CTCTTC 7 cut(s) 256, 577, 1179, 1850, 2289, 2444, 2525
Eam1105I GACNNNNNGTC 1 cut(s) 3012
EarI CTCTTC 7 cut(s) 256, 577, 1179, 1850, 2289, 2444, 2525
Ecl136II GAGCTC 2 cut(s) 437, 2515
Eco130I CCWWGG 3 cut(s) 237, 2169, 3009
Eco24I GRGCYC 3 cut(s) 439, 570, 2517
Eco31I GGTCTC 1 cut(s) 2118
Eco32I GATATC 1 cut(s) 811
Eco47I GGWCC 3 cut(s) 1534, 3024, 3410
Eco47III AGCGCT 2 cut(s) 404, 2674
Eco53kI GAGCTC 2 cut(s) 437, 2515
Eco81I CCTNAGG 3 cut(s) 1110, 1530, 1937
Eco88I CYCGRG 2 cut(s) 2465, 3405
EcoICRI GAGCTC 2 cut(s) 437, 2515
EcoNI CCTNNNNNAGG 1 cut(s) 1113
EcoO109I RGGNCCY 2 cut(s) 1534, 1615
EcoRI GAATTC 2 cut(s) 2926, 3170
EcoRII CCWGG 5 cut(s) 967, 1537, 2768, 2784, 2953
EcoRV GATATC 1 cut(s) 811
EcoT14I CCWWGG 3 cut(s) 237, 2169, 3009
EcoT22I ATGCAT 3 cut(s) 58, 1482, 3150
EcoT38I GRGCYC 3 cut(s) 439, 570, 2517
ErhI CCWWGG 3 cut(s) 237, 2169, 3009
FalI AAGNNNNNCTT 4 cut(s) 2329, 2361, 3193, 3225
FaqI GGGAC 2 cut(s) 161, 3134
FbaI TGATCA 1 cut(s) 2745
FblI GTMKAC 1 cut(s) 2381
Fnu4HI GCNGC 1 cut(s) 733
FokI GGATG 5 cut(s) 478, 951, 1092, 2272, 2886
FriOI GRGCYC 3 cut(s) 439, 570, 2517
Fsp4HI GCNGC 1 cut(s) 733
FspAI RTGCGCAY 2 cut(s) 5, 1059
FspBI CTAG 5 cut(s) 729, 993, 1430, 1551, 1835
FspI TGCGCA 2 cut(s) 5, 1059
GlaI GCGC 4 cut(s) 5, 404, 1059, 2674
GluI GCNGC 1 cut(s) 733
GsuI CTGGAG 1 cut(s) 3255
HaeII RGCGCY 2 cut(s) 406, 2676
HaeIII GGCC 7 cut(s) 1617, 1935, 2272, 2703, 3216, 3373, 3404
HapII CCGG 6 cut(s) 40, 1652, 1959, 2663, 3160, 3197
HgaI GACGC 1 cut(s) 3368
HhaI GCGC 4 cut(s) 6, 405, 1060, 2675
Hin6I GCGC 4 cut(s) 4, 403, 1058, 2673
HinP1I GCGC 4 cut(s) 4, 403, 1058, 2673
HincII GTYRAC 2 cut(s) 1473, 2226
HindII GTYRAC 2 cut(s) 1473, 2226
HindIII AAGCTT 6 cut(s) 797, 1889, 2084, 2147, 2332, 2524
HpaI GTTAAC 1 cut(s) 1473
HpaII CCGG 6 cut(s) 40, 1652, 1959, 2663, 3160, 3197
HphI GGTGA 4 cut(s) 95, 1518, 2677, 3464
Hpy166II GTNNAC 9 cut(s) 45, 784, 1473, 2226, 2315, 2382, 3024, 3190, 3273
Hpy8I GTNNAC 9 cut(s) 45, 784, 1473, 2226, 2315, 2382, 3024, 3190, 3273
Hpy99I CGWCG 1 cut(s) 1523
HpyAV CCTTC 9 cut(s) 845, 1040, 1102, 1109, 1497, 2767, 2904, 3055, 3219
HpyF10VI GCNNNNNNNGC 8 cut(s) 10, 458, 796, 1486, 1783, 2811, 3446, 3455
HspAI GCGC 4 cut(s) 4, 403, 1058, 2673
Kpn2I TCCGGA 1 cut(s) 1958
Ksp22I TGATCA 1 cut(s) 2745
KspAI GTTAAC 1 cut(s) 1473
Kzo9I GATC 9 cut(s) 100, 626, 864, 1187, 1312, 2374, 2594, 2745, 2870
LguI GCTCTTC 2 cut(s) 1179, 1850
LmnI GCTCC 4 cut(s) 187, 1126, 2520, 2782
Lsp1109I GCAGC 1 cut(s) 744
MaeI CTAG 5 cut(s) 729, 993, 1430, 1551, 1835
MaeIII GTNAC 5 cut(s) 210, 1118, 1405, 3229, 3243
MalI GATC 9 cut(s) 102, 628, 866, 1189, 1314, 2376, 2596, 2747, 2872
MboI GATC 9 cut(s) 100, 626, 864, 1187, 1312, 2374, 2594, 2745, 2870
MfeI CAATTG 1 cut(s) 1484
MflI RGATCY 2 cut(s) 1187, 2870
MhlI GDGCHC 6 cut(s) 47, 439, 570, 1626, 2517, 3306
MlsI TGGCCA 2 cut(s) 2272, 3216
MluNI TGGCCA 2 cut(s) 2272, 3216
MlyI GAGTC 6 cut(s) 218, 497, 1652, 2275, 2461, 2464
MmeI TCCRAC 2 cut(s) 619, 2340
Mox20I TGGCCA 2 cut(s) 2272, 3216
Mph1103I ATGCAT 3 cut(s) 58, 1482, 3150
MroI TCCGGA 1 cut(s) 1958
MroXI GAANNNNTTC 4 cut(s) 1713, 1755, 2127, 2505
MscI TGGCCA 2 cut(s) 2272, 3216
MslI CAYNNNNRTG 2 cut(s) 1929, 2163
Msp20I TGGCCA 2 cut(s) 2272, 3216
MspCI CTTAAG 2 cut(s) 1988, 2335
MspI CCGG 6 cut(s) 40, 1652, 1959, 2663, 3160, 3197
MspR9I CCNGG 6 cut(s) 969, 1539, 2770, 2786, 2955, 3161
MunI CAATTG 1 cut(s) 1484
Mva1269I GAATGC 4 cut(s) 25, 56, 954, 1129
MvaI CCWGG 5 cut(s) 969, 1539, 2770, 2786, 2955
MvnI CGCG 1 cut(s) 1518
MwoI GCNNNNNNNGC 8 cut(s) 10, 458, 796, 1486, 1783, 2811, 3446, 3455
NciI CCSGG 1 cut(s) 3161
NcoI CCATGG 1 cut(s) 2169
NdeII GATC 9 cut(s) 100, 626, 864, 1187, 1312, 2374, 2594, 2745, 2870
NheI GCTAGC 1 cut(s) 728
NmuCI GTSAC 2 cut(s) 210, 3243
NruI TCGCGA 1 cut(s) 1518
NsbI TGCGCA 2 cut(s) 5, 1059
NsiI ATGCAT 3 cut(s) 58, 1482, 3150
NspI RCATGY 4 cut(s) 613, 1058, 1091, 1874
NspV TTCGAA 2 cut(s) 1674, 3417
PaeI GCATGC 1 cut(s) 1091
PaeR7I CTCGAG 1 cut(s) 2465
PagI TCATGA 3 cut(s) 1018, 1315, 2158
PciI ACATGT 2 cut(s) 609, 1870
PciSI GCTCTTC 2 cut(s) 1179, 1850
PcsI WCGNNNNNNNCGW 1 cut(s) 877
PctI GAATGC 4 cut(s) 25, 56, 954, 1129
PdmI GAANNNNTTC 4 cut(s) 1713, 1755, 2127, 2505
PflFI GACNNNGTC 1 cut(s) 1945
PfoI TCCNGGA 1 cut(s) 967
PinAI ACCGGT 2 cut(s) 39, 2662
PkrI GCNGC 1 cut(s) 734
PleI GAGTC 6 cut(s) 217, 496, 1651, 2275, 2460, 2464
PpsI GAGTC 6 cut(s) 217, 496, 1651, 2275, 2460, 2464
Ppu21I YACGTR 2 cut(s) 48, 3131
PpuMI RGGWCCY 1 cut(s) 1534
PscI ACATGT 2 cut(s) 609, 1870
PshAI GACNNNNGTC 1 cut(s) 2221
PshBI ATTAAT 1 cut(s) 2001
PsiI TTATAA 1 cut(s) 702
Psp124BI GAGCTC 2 cut(s) 439, 2517
Psp1406I AACGTT 1 cut(s) 1578
Psp5II RGGWCCY 1 cut(s) 1534
Psp6I CCWGG 5 cut(s) 967, 1537, 2768, 2784, 2953
PspGI CCWGG 5 cut(s) 967, 1537, 2768, 2784, 2953
PspPI GGNCC 7 cut(s) 1534, 1615, 2701, 3024, 3371, 3403, 3410
PspPPI RGGWCCY 1 cut(s) 1534
PsrI GAACNNNNNNTAC 4 cut(s) 174, 206, 861, 893
PsuI RGATCY 2 cut(s) 1187, 2870
PsyI GACNNNGTC 1 cut(s) 1945
RruI TCGCGA 1 cut(s) 1518
RseI CAYNNNNRTG 2 cut(s) 1929, 2163
SacI GAGCTC 2 cut(s) 439, 2517
SapI GCTCTTC 2 cut(s) 1179, 1850
SatI GCNGC 1 cut(s) 733
Sau3AI GATC 9 cut(s) 100, 626, 864, 1187, 1312, 2374, 2594, 2745, 2870
Sau96I GGNCC 7 cut(s) 1534, 1615, 2701, 3024, 3371, 3403, 3410
SchI GAGTC 6 cut(s) 218, 497, 1652, 2275, 2461, 2464
ScrFI CCNGG 6 cut(s) 969, 1539, 2770, 2786, 2955, 3161
SduI GDGCHC 6 cut(s) 47, 439, 570, 1626, 2517, 3306
SfcI CTRYAG 1 cut(s) 109
Sfr274I CTCGAG 1 cut(s) 2465
SfuI TTCGAA 2 cut(s) 1674, 3417
SinI GGWCC 3 cut(s) 1534, 3024, 3410
SlaI CTCGAG 1 cut(s) 2465
SmiMI CAYNNNNRTG 2 cut(s) 1929, 2163
SmlI CTYRAG 6 cut(s) 61, 1973, 1988, 2117, 2335, 2465
SmoI CTYRAG 6 cut(s) 61, 1973, 1988, 2117, 2335, 2465
SphI GCATGC 1 cut(s) 1091
SsiI CCGC 1 cut(s) 3456
SspI AATATT 2 cut(s) 2209, 2549
SspMI CTAG 5 cut(s) 729, 993, 1430, 1551, 1835
SstI GAGCTC 2 cut(s) 439, 2517
StyD4I CCNGG 6 cut(s) 967, 1537, 2768, 2784, 2953, 3159
StyI CCWWGG 3 cut(s) 237, 2169, 3009
TaqI TCGA 6 cut(s) 867, 1674, 2466, 2499, 2739, 3417
TatI WGTACW 3 cut(s) 2552, 2762, 3261
TscAI CASTG 4 cut(s) 118, 2170, 2310, 2737
TseFI GTSAC 2 cut(s) 210, 3243
TseI GCWGC 1 cut(s) 732
Tsp45I GTSAC 2 cut(s) 210, 3243
TspGWI ACGGA 3 cut(s) 208, 1867, 1896
TspRI CASTG 4 cut(s) 118, 2170, 2310, 2737
Tth111I GACNNNGTC 1 cut(s) 1945
Vha464I CTTAAG 2 cut(s) 1988, 2335
VneI GTGCAC 1 cut(s) 43
VpaK11BI GGWCC 3 cut(s) 1534, 3024, 3410
VspI ATTAAT 1 cut(s) 2001
XagI CCTNNNNNAGG 1 cut(s) 1113
XapI RAATTY 8 cut(s) 719, 1765, 1908, 2502, 2575, 2926, 3170, 3359
XceI RCATGY 4 cut(s) 613, 1058, 1091, 1874
XcmI CCANNNNNNNNNTGG 5 cut(s) 1822, 2032, 2250, 2302, 3092
XhoI CTCGAG 1 cut(s) 2465
XmiI GTMKAC 1 cut(s) 2381
XmnI GAANNNNTTC 4 cut(s) 1713, 1755, 2127, 2505
XspI CTAG 5 cut(s) 729, 993, 1430, 1551, 1835
Zsp2I ATGCAT 3 cut(s) 58, 1482, 3150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.