Rh6BG295500

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000654
Physical Location & Seq
Reverse (-)
52432546 .. 52433517
972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG295500.1

Sequence Viewer

Length: 828 bp
ATGATAAAATATATGGGTCTGTACAATTTGGAATCCACTGAAAGCATTGAAGTCAAAATGAGCAATCAGCTGACATTTACAGACAAGACAGGTCCACTCCAGGGACTCTATGAATGTGGAATATTTAGCATTTTCCTGCATGGAAGCACAATTCCTGACTGCTACACCTACAGAAGCATGGGTGATTCAGTGTTGTCTATAACTGTTCCTTCCCATCTTAAGCTTAAGATCCGAGGCTTAAATGTATGTATTCTATATGCACTAGGCCCATGCCACCACCTTAAAGTCAGTAATGACACGAAGGGTCTTATGTGGACATACTGCCCAGTCACTGCAGGTGTTCCTAAAGAGGATGGACTGATGCTATGGCTAAGTCATTGGCTGTTTGAGAACCATGAGTTGGAGGCTGGGGATGAATTACGTGTCTCGGTGCATAATGGAGCTATATTCTTTGACCCTCTTGGTCGTCAAGACGTTCTTCCAGCAAAGGAATTTGGAATCCAACTTGTGTATGAATCGGAAAATAAGGACGTCCGATCCAAAAGTGAAGATCTTACGGTACAACATGAAGCGCCTTATTGGAGTTACAATATTGTTACTGGGAATGGATCATTGTCAGCATCAAAGTATCAAATGTGGACAGGCAAATACTTTCTTTGCAACTGTGTTCACAATATGCGTCAGATTCATATCAGAAATTGCGAGGAGAATCCAGCTCATCTTGATTTTTCATATGAGCCCAAAGATCGTTTGAGTGGATATTGCCGTCATTTATTTGATCACACTCACTTTCACAAGAAAAAATTTGAGTTTGTATTGAATAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

275

Amino Acids

31.65

Weight (kDa)

6.42

Isoelectric Point (pI)

37.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 46 - 174 3.7e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 326
AatII GACGTC 1 cut(s) 534
Acc36I ACCTGC 1 cut(s) 326
AccB7I CCANNNNNTGG 1 cut(s) 400
AclWI GGATC 3 cut(s) 223, 531, 616
AcsI RAATTY 2 cut(s) 491, 803
AcyI GRCGYC 1 cut(s) 531
AfaI GTAC 2 cut(s) 23, 561
AfiI CCNNNNNNNGG 2 cut(s) 101, 400
AflII CTTAAG 2 cut(s) 218, 224
AflIII ACRYGT 1 cut(s) 421
AgsI TTSAA 2 cut(s) 50, 820
AjnI CCWGG 1 cut(s) 99
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AluBI AGCT 4 cut(s) 70, 223, 443, 716
AluI AGCT 4 cut(s) 70, 223, 443, 716
Alw26I GTCTC 1 cut(s) 430
AlwI GGATC 3 cut(s) 223, 531, 616
AlwNI CAGNNNCTG 1 cut(s) 332
AoxI GGCC 1 cut(s) 265
ApoI RAATTY 2 cut(s) 491, 803
AspLEI GCGC 1 cut(s) 574
AspS9I GGNCC 2 cut(s) 92, 266
AsuHPI GGTGA 1 cut(s) 194
AvaII GGWCC 1 cut(s) 92
BanII GRGCYC 1 cut(s) 741
BccI CCATC 2 cut(s) 222, 347
BceAI ACGGC 1 cut(s) 750
BciT130I CCWGG 1 cut(s) 101
BclI TGATCA 1 cut(s) 778
BcoDI GTCTC 1 cut(s) 430
BfaI CTAG 1 cut(s) 263
BfmI CTRYAG 2 cut(s) 169, 333
BfoI RGCGCY 1 cut(s) 575
BfrI CTTAAG 2 cut(s) 218, 224
BfuAI ACCTGC 1 cut(s) 326
BglII AGATCT 1 cut(s) 550
Bme1390I CCNGG 1 cut(s) 101
Bme18I GGWCC 1 cut(s) 92
BmgT120I GGNCC 2 cut(s) 92, 266
BmrFI CCNGG 1 cut(s) 101
BmrI ACTGGG 2 cut(s) 320, 609
BmsI GCATC 2 cut(s) 351, 629
BmuI ACTGGG 2 cut(s) 320, 609
BpmI CTGGAG 1 cut(s) 83
BsaAI YACGTR 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 689
BsaHI GRCGYC 1 cut(s) 531
BsaJI CCNNGG 2 cut(s) 100, 232
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 400
Bse1I ACTGG 2 cut(s) 326, 604
Bse8I GATNNNNATC 1 cut(s) 689
BseBI CCWGG 1 cut(s) 101
BseDI CCNNGG 2 cut(s) 100, 232
BseGI GGATG 2 cut(s) 358, 418
BseJI GATNNNNATC 1 cut(s) 689
BseLI CCNNNNNNNGG 2 cut(s) 101, 400
BseNI ACTGG 2 cut(s) 326, 604
BseRI GAGGAG 1 cut(s) 719
BseYI CCCAGC 1 cut(s) 407
BshFI GGCC 1 cut(s) 267
BslFI GGGAC 1 cut(s) 117
BslI CCNNNNNNNGG 2 cut(s) 101, 400
BsmAI GTCTC 1 cut(s) 430
BsmFI GGGAC 1 cut(s) 117
BsnI GGCC 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 741
Bsp1407I TGTACA 1 cut(s) 21
Bsp143I GATC 6 cut(s) 228, 536, 550, 608, 745, 778
BspANI GGCC 1 cut(s) 267
BspMAI CTGCAG 1 cut(s) 337
BspMI ACCTGC 1 cut(s) 326
BspPI GGATC 3 cut(s) 223, 531, 616
BspTI CTTAAG 2 cut(s) 218, 224
BsrGI TGTACA 1 cut(s) 21
BsrI ACTGG 2 cut(s) 326, 604
BssECI CCNNGG 2 cut(s) 100, 232
BssMI GATC 6 cut(s) 228, 536, 550, 608, 745, 778
BssNI GRCGYC 1 cut(s) 531
Bst2UI CCWGG 1 cut(s) 101
Bst4CI ACNGT 3 cut(s) 205, 559, 665
BstACI GRCGYC 1 cut(s) 531
BstAFI CTTAAG 2 cut(s) 218, 224
BstAUI TGTACA 1 cut(s) 21
BstBAI YACGTR 1 cut(s) 422
BstDEI CTNAG 1 cut(s) 371
BstF5I GGATG 2 cut(s) 358, 418
BstH2I RGCGCY 1 cut(s) 575
BstHHI GCGC 1 cut(s) 574
BstKTI GATC 6 cut(s) 231, 539, 553, 611, 748, 781
BstMAI GTCTC 1 cut(s) 430
BstMBI GATC 6 cut(s) 228, 536, 550, 608, 745, 778
BstNI CCWGG 1 cut(s) 101
BstSCI CCNGG 1 cut(s) 99
BstSFI CTRYAG 2 cut(s) 169, 333
BstX2I RGATCY 2 cut(s) 228, 550
BstYI RGATCY 2 cut(s) 228, 550
BsuRI GGCC 1 cut(s) 267
BtsCI GGATG 2 cut(s) 358, 418
BtsI GCAGTG 1 cut(s) 330
BtsIMutI CAGTG 3 cut(s) 36, 195, 330
BveI ACCTGC 1 cut(s) 326
CaiI CAGNNNCTG 1 cut(s) 332
CfoI GCGC 1 cut(s) 574
Cfr13I GGNCC 2 cut(s) 92, 266
CseI GACGC 1 cut(s) 668
Csp6I GTAC 2 cut(s) 22, 560
CviAII CATG 5 cut(s) 140, 178, 270, 395, 566
CviQI GTAC 2 cut(s) 22, 560
DdeI CTNAG 1 cut(s) 371
DpnI GATC 6 cut(s) 230, 538, 552, 610, 747, 780
DpnII GATC 6 cut(s) 228, 536, 550, 608, 745, 778
Eco24I GRGCYC 1 cut(s) 741
Eco47I GGWCC 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 99
EcoT38I GRGCYC 1 cut(s) 741
FaeI CATG 5 cut(s) 143, 181, 273, 398, 569
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FaqI GGGAC 1 cut(s) 117
FatI CATG 5 cut(s) 139, 177, 269, 394, 565
FauNDI CATATG 1 cut(s) 733
FbaI TGATCA 1 cut(s) 778
FokI GGATG 2 cut(s) 365, 425
FriOI GRGCYC 1 cut(s) 741
FspBI CTAG 1 cut(s) 263
GlaI GCGC 1 cut(s) 573
GsaI CCCAGC 1 cut(s) 411
GsuI CTGGAG 1 cut(s) 83
HaeII RGCGCY 1 cut(s) 575
HaeIII GGCC 1 cut(s) 267
HgaI GACGC 1 cut(s) 668
HhaI GCGC 1 cut(s) 574
Hin1I GRCGYC 1 cut(s) 531
Hin1II CATG 5 cut(s) 143, 181, 273, 398, 569
Hin6I GCGC 1 cut(s) 572
HinP1I GCGC 1 cut(s) 572
HindIII AAGCTT 1 cut(s) 221
HinfI GANTC 7 cut(s) 32, 105, 185, 498, 515, 685, 709
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 4 cut(s) 95, 315, 639, 670
Hpy188I TCNGA 5 cut(s) 233, 520, 536, 684, 695
Hpy188III TCNNGA 3 cut(s) 155, 470, 722
Hpy8I GTNNAC 4 cut(s) 95, 315, 639, 670
HpyAV CCTTC 2 cut(s) 219, 295
HpyCH4III ACNGT 3 cut(s) 205, 559, 665
HpyCH4IV ACGT 3 cut(s) 421, 474, 531
HpyCH4V TGCA 5 cut(s) 139, 260, 335, 433, 660
HpyF3I CTNAG 1 cut(s) 371
HpySE526I ACGT 3 cut(s) 421, 474, 531
Hsp92I GRCGYC 1 cut(s) 531
Hsp92II CATG 5 cut(s) 143, 181, 273, 398, 569
HspAI GCGC 1 cut(s) 572
Ksp22I TGATCA 1 cut(s) 778
Kzo9I GATC 6 cut(s) 228, 536, 550, 608, 745, 778
LmnI GCTCC 1 cut(s) 440
LweI GCATC 2 cut(s) 351, 629
MaeI CTAG 1 cut(s) 263
MaeII ACGT 3 cut(s) 421, 474, 531
MaeIII GTNAC 3 cut(s) 328, 584, 595
MalI GATC 6 cut(s) 230, 538, 552, 610, 747, 780
MboI GATC 6 cut(s) 228, 536, 550, 608, 745, 778
MboII GAAGA 2 cut(s) 470, 560
MflI RGATCY 2 cut(s) 228, 550
MhlI GDGCHC 1 cut(s) 741
MluCI AATT 6 cut(s) 25, 150, 416, 491, 697, 803
MlyI GAGTC 1 cut(s) 99
MmeI TCCRAC 2 cut(s) 381, 526
MnlI CCTC 5 cut(s) 227, 343, 397, 468, 697
MseI TTAA 4 cut(s) 219, 225, 239, 282
MspA1I CMGCKG 1 cut(s) 70
MspCI CTTAAG 2 cut(s) 218, 224
MspR9I CCNGG 1 cut(s) 101
MvaI CCWGG 1 cut(s) 101
NdeI CATATG 1 cut(s) 733
NdeII GATC 6 cut(s) 228, 536, 550, 608, 745, 778
NlaIII CATG 5 cut(s) 143, 181, 273, 398, 569
NmuCI GTSAC 1 cut(s) 328
PaqCI CACCTGC 1 cut(s) 326
PfeI GAWTC 6 cut(s) 32, 185, 498, 515, 685, 709
PflMI CCANNNNNTGG 1 cut(s) 400
PleI GAGTC 1 cut(s) 99
PpsI GAGTC 1 cut(s) 99
Ppu21I YACGTR 1 cut(s) 422
Psp6I CCWGG 1 cut(s) 99
PspFI CCCAGC 1 cut(s) 407
PspGI CCWGG 1 cut(s) 99
PspPI GGNCC 2 cut(s) 92, 266
PstI CTGCAG 1 cut(s) 337
PstNI CAGNNNCTG 1 cut(s) 332
PsuI RGATCY 2 cut(s) 228, 550
PvuII CAGCTG 1 cut(s) 70
RsaI GTAC 2 cut(s) 23, 561
RsaNI GTAC 2 cut(s) 22, 560
SaqAI TTAA 4 cut(s) 219, 225, 239, 282
Sau3AI GATC 6 cut(s) 228, 536, 550, 608, 745, 778
Sau96I GGNCC 2 cut(s) 92, 266
SchI GAGTC 1 cut(s) 99
ScrFI CCNGG 1 cut(s) 101
SduI GDGCHC 1 cut(s) 741
SfaNI GCATC 2 cut(s) 351, 629
SfcI CTRYAG 2 cut(s) 169, 333
SinI GGWCC 1 cut(s) 92
SmlI CTYRAG 2 cut(s) 218, 224
SmoI CTYRAG 2 cut(s) 218, 224
Sse9I AATT 6 cut(s) 25, 150, 416, 491, 697, 803
SspI AATATT 2 cut(s) 123, 592
SspMI CTAG 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 99
TaaI ACNGT 3 cut(s) 205, 559, 665
TaiI ACGT 3 cut(s) 424, 477, 534
TasI AATT 6 cut(s) 25, 150, 416, 491, 697, 803
TatI WGTACW 1 cut(s) 21
TfiI GAWTC 6 cut(s) 32, 185, 498, 515, 685, 709
Tru1I TTAA 4 cut(s) 219, 225, 239, 282
Tru9I TTAA 4 cut(s) 219, 225, 239, 282
TscAI CASTG 3 cut(s) 43, 195, 337
TseFI GTSAC 1 cut(s) 328
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 6 cut(s) 126, 429, 528, 582, 677, 720
TspRI CASTG 3 cut(s) 43, 195, 337
Van91I CCANNNNNTGG 1 cut(s) 400
Vha464I CTTAAG 2 cut(s) 218, 224
VpaK11BI GGWCC 1 cut(s) 92
XapI RAATTY 2 cut(s) 491, 803
XspI CTAG 1 cut(s) 263
ZraI GACGTC 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.