MD15G1376300.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000654
Physical Location & Seq
Forward (+)
46022732 .. 46029184
6453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1376300.v1.1.491

Sequence Viewer

Length: 3459 bp
ATGGCGTCGAAGGGTAAGGGGATCGCCGCAGGAGGTGGATTCTCCGGCGGGAAGCGTAAGCACGATGACAAGTCCGGTGGTGGCCGGAAGAAGAGCAACCCCGGCGTCCTTAGATTCTTCGAAGACGTCGCTGACGAAGCCGACGACGGCGACGAGAGTGACTTCGATGATGATAGACTCTATTCAATAGCTGCAATGAGCAACGAGCAGCAGAATCCTTCTTCGTCTTCTAACCGGTGCACATATGATGCATTCTTGAGTTTCAGAGGCGCAGATACACGCAAGGGCTTTACAGATCACCTCTACAATGCTTTGAAGTTAGCAGGAATCCACACTTTTAGGGATGATGATGAAATTGAGAGAGGAGAAAACATTGCACAGGAGCTACAGACAGCAATACAAGAGTCACAAGTATCAATCATTGTTTTCTCCAAGGACTACGCCTCTTCCAGGTGGTGCCTGAATGAACTTGCTATCATCATGGAACGTAAAAGAACAGATGGACACATGGTTATCCCAGTTTTCTATTATGTGGAACCATCAGATGTCAGGAAGCAGACGGGCAGTTTTGCAGAATCATTTACTAGACATCAAGAACAATTCAAGGACGAGATAGACAAGGTGGAGGAGTGGAGGCGAGCTCTTAGAGATGTTGCAGACTTGGGAGGGATGGTTTTAAAAGACCGGTACGAGTCGCAGTTTATCCAAGAAATTGTTGAAGCTGTTGGAAAAAAACTGGACTACATGACAAATTGGAGACTAAGAGTTGAGCCCTATGTGATTGGAAGAGATTATCATGTGAAAAGACTAAACATGTGGCTGGAAGATGGATCAAATGATGTTGGAGTAGCTGTCATCCATGCAATGGGTGGAATAGGCAAGACCACCATTGCTAAAATTGCTTATAACCAGAACTTCAATAAATTTGAAGCTAGCAGCTTTCTTTCAGATATTAGGGAAACTTCCGGACAAGCCAATGGTTTTGTTCGCTTGCAAAGGAGACTTCTTTCAGATATCCAAAAGAGGAAAATGGAAAAAATAGACGGGCCTGAAGGTCTAGTAAAGATCAAACGGGTTGTACGTTGCAAAAGAGTTCTTATTGTTCTTGATGATGTGGAGAACTCGGAGCAGTTCAATGCAATTCTTGGAATGCGAGACTGGTTCTATCCTGGAAGTAAAATTATAATAACAACTAGGCATGAACATTTGTTAAAGGCTCATGAAGTTGTAAGGTTTAAGGTTGAAGGATTGCATGAGGATGAATCACTTAAGCTTTTCAGTTGGCACGCCTTTGGACAACCCCGTCCTCAAGAAGGTTACATGGAGCTTTCAAGACCCATAGTAGAACATTGTGGAGGGGTTCCATTAGCTCTTAAAGTTCTGGGATCTTCTCTATTTGGAAAAACAGCAGATATATGGGAAAATGCAATACACAACTTAGACGAGTTTACTGAGGGAAAAATTCGAAAGATTCTTTGCATAAGTTTTGATTCTTTACAAGATCATGATAAACGTTTATTCCTCAATATAGCCTGTTTCTTCGTGGGAAAGAACAAGGATTTTACAACTACAATCCTTGACGAATGTGATTTTGCCACAAAGGTTGGACTTCAACGTCTGGTTGATAGATGTCTTGTGGAAATTAATGTCTACAACAAGTTAACCATGCATCAATTGCTTCAAGACATGGGAAGGGCAATAATTCGCGAAGAATCACCTGAGGACCCTGGAAAACGCACTAGACTGTGGCATAAAGATGCAATTAACGTTTTGAGAAAATTAACGGGTACGAGAAGTATCAAGGGCCTCATGCTCAACTTTCCCTCAACAGATTCATCCCCAGTATTAAATGAGGAAGGTTTCGAAACAAAGGCATTTACAGAGATGCTCAATCTTGAACTACTTCTGCTTGATAATGTAAAGTTGAGTGGAAGCTATGAAGATTTTCCAAAAAATTTAATATTGCTGTCTTGGCGAGGATTCTCTTTAAAATCAATACCAGCCAATTTTTGTTTGCGAAATCTAGTTGTTCTTGACTTACGGAAGAGCAGTCTGCAACATGTTTGGAGAGGAACCAAGAATCTTAAAAGATTGAAAATTCTTAATCTCAGTCATTCACATGGCCTTAGGACAACCTCTGATTTGTCCATGCTCACTAACCTTGAGAAACTAATTCTTAAGGATTGCATTAATTTGGTTGATGTTGATGAATCCATTGGAAACTTGGGGAAACTTGTTTTCTTGAATCTAAAAGACTGCAAAAGCCTTACGAAGCTTCCAAAAAGAATGATCATGTTGAGGTCTCTTGAGGAACTTAATCTTAGCGGTTGCTCAAAGCTTGTGCTTCATGACAGTGCCACAGTTATTCATTTACACGCAATATCTAGGGATATGAATAGACCTAGACTGTTGTCAACTTTATCATGGACACCAATCGGGGCTTGGAGGATGTGGGCATGGCCGGGAAAGACTCTTCAATCAACCAGTTTCTCACTGGCAAGTTTACCACGTTTTTTGGGAAGCTTAAATCTGTCTGATTGCAATCTGTCAGAGGTTCCCAATGATCTGTGTACACTATCTTCGTTGAAGCATTTGAATCTAAATAGTAACCCAATTTTGTGCCTACCACAAAACATGAAGAGTCTTATTATGCTTGAGACTCTTTCGTTAGAAGATTGCACAAACCTCGAAATGCTTCCAGAGCTCCCAGCAAGGTTGGAATCGTTGGTACCACATGGTTGTAAATCATTGAAAAGATTAACAAATTTACCGAACTTGTTTAAATTGGATTTGAATTTTGGTTGTTGCCCTCTGTTAATTGAAGTTGAAAGCTTGTTGAATAAAAAAACGTTGATGTTGTTCAATATGGAATCCATAGCAAGCACTGATGACGAAATGCTCGACAACTTGACCGATACAACCAGGAAGGTTCCTGTCCAGGTATTCTTTCACGGTCGCACATATAGCATTTTCCTTCATGCAAGCAAGATTCCAGATGGATTTGACTTCACAACACATAAACCTGTGCTGTCATTTATTCCACCTTCACATCCTAATGGCAAAAGCTTGAATGTAGCTGTTTTATATGCCAAAAATGAACATCAGATTAAAGCCAGTGGACTAAATATGATTCACGTTCAAGTTTTTCATGAGATGGGGGGGAGTACTGGGTCGACCCATTCGGTACTCACAGTAGGTTTGCCAACTGAGAATGGAGATGTGTTGTGGCTTTGCCATATGCAATTTCAAAGCAAGGAAAGGGAATGTGTACGTGTTTCGGTGGAGATGAGTGATTTTTACCGGGTGAAGGAGTTGGGAATCCAGCTTTTGTACGCGCAGAATGATGTTGTTGCTGGACATGTGTCACTGTCAGCATCACCGTACGGCTACAGAATGGGGCCATTAAGTTACTATGGCCGCCTTCCTCGAACTTGTTTCATTGAGAAAGTAGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1153

Amino Acids

130.46

Weight (kDa)

7.38

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 82 - 251 1.6e-54 TIR domain
TIR_2 PF13676 85 - 180 6.3e-17 TIR domain
NB-ARC PF00931 264 - 431 4.5e-21 NB-ARC domain
WHD_ROQ1 PF23282 502 - 572 1.2e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 665 - 777 6.9e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 906, 1184
AasI GACNNNNNNGTC 2 cut(s) 1302, 1614
AatII GACGTC 1 cut(s) 129
Acc65I GGTACC 1 cut(s) 2728
AccB1I GGYRCC 2 cut(s) 456, 2728
AccB7I CCANNNNNTGG 1 cut(s) 865
AccI GTMKAC 2 cut(s) 1650, 3173
AccII CGCG 2 cut(s) 1707, 3335
AccIII TCCGGA 1 cut(s) 965
AciI CCGC 4 cut(s) 27, 48, 2325, 3418
AclI AACGTT 3 cut(s) 1513, 1767, 2849
AclWI GGATC 3 cut(s) 29, 838, 1393
AcoI YGGCCR 3 cut(s) 82, 2459, 3415
AcsI RAATTY 6 cut(s) 923, 1461, 1954, 2097, 2764, 2794
AcuI CTGAAG 1 cut(s) 1071
AcyI GRCGYC 3 cut(s) 5, 105, 126
AfiI CCNNNNNNNGG 4 cut(s) 450, 865, 1313, 3307
AflII CTTAAG 2 cut(s) 1268, 2177
AflIII ACRYGT 4 cut(s) 813, 2059, 3271, 3358
AgeI ACCGGT 2 cut(s) 234, 684
AjnI CCWGG 5 cut(s) 449, 1168, 1726, 2921, 2937
Alw21I GWGCWC 3 cut(s) 242, 643, 2706
Alw26I GTCTC 5 cut(s) 751, 994, 1149, 2307, 2651
Alw44I GTGCAC 1 cut(s) 238
AlwI GGATC 3 cut(s) 29, 838, 1393
Aor13HI TCCGGA 1 cut(s) 965
AoxI GGCC 7 cut(s) 82, 1046, 1804, 2122, 2459, 3398, 3415
ApaLI GTGCAC 1 cut(s) 238
ApeKI GCWGC 3 cut(s) 191, 208, 936
ApoI RAATTY 6 cut(s) 923, 1461, 1954, 2097, 2764, 2794
AseI ATTAAT 2 cut(s) 1644, 2190
AsiGI ACCGGT 2 cut(s) 234, 684
Asp700I GAANNNNTTC 3 cut(s) 1902, 1944, 2694
Asp718I GGTACC 1 cut(s) 2728
AspLEI GCGC 2 cut(s) 272, 3337
AspS9I GGNCC 4 cut(s) 1046, 1723, 1804, 3398
AsuC2I CCSGG 3 cut(s) 102, 2463, 3302
AsuHPI GGTGA 4 cut(s) 290, 1707, 3316, 3369
AsuII TTCGAA 3 cut(s) 120, 1465, 1863
AsuNHI GCTAGC 1 cut(s) 932
AvaII GGWCC 1 cut(s) 1723
AxyI CCTNAGG 2 cut(s) 1719, 2126
BaeGI GKGCMC 1 cut(s) 242
BanI GGYRCC 2 cut(s) 456, 2728
BanII GRGCYC 3 cut(s) 643, 774, 2706
BarI GAAGNNNNNNTAC 2 cut(s) 2563, 2595
BbsI GAAGAC 2 cut(s) 129, 219
Bbv12I GWGCWC 3 cut(s) 242, 643, 2706
BbvI GCAGC 3 cut(s) 178, 220, 948
BccI CCATC 6 cut(s) 494, 547, 664, 821, 2990, 3148
BceAI ACGGC 2 cut(s) 163, 3400
BcgI CGANNNNNNTGC 2 cut(s) 2668, 2702
BciT130I CCWGG 5 cut(s) 451, 1170, 1728, 2923, 2939
BclI TGATCA 1 cut(s) 2289
BcnI CCSGG 3 cut(s) 102, 2463, 3302
BcoDI GTCTC 5 cut(s) 751, 994, 1149, 2307, 2651
BfaI CTAG 8 cut(s) 585, 933, 1058, 1194, 1740, 2024, 2385, 2403
BfmI CTRYAG 2 cut(s) 386, 3388
BfrI CTTAAG 2 cut(s) 1268, 2177
BisI GCNGC 5 cut(s) 27, 192, 209, 937, 3418
BlsI GCNGC 5 cut(s) 28, 193, 210, 938, 3419
BmcAI AGTACT 1 cut(s) 3166
Bme1390I CCNGG 8 cut(s) 102, 451, 1170, 1728, 2463, 2923, 2939, 3302
Bme18I GGWCC 1 cut(s) 1723
BmgT120I GGNCC 4 cut(s) 1046, 1723, 1804, 3398
BmiI GGNNCC 9 cut(s) 458, 537, 1362, 1725, 2074, 2556, 2730, 2931, 3399
BmrFI CCNGG 8 cut(s) 102, 451, 1170, 1728, 2463, 2923, 2939, 3302
BmrI ACTGGG 3 cut(s) 512, 1835, 3177
BmsI GCATC 5 cut(s) 238, 1678, 1747, 1875, 3383
BmtI GCTAGC 1 cut(s) 936
BmuI ACTGGG 3 cut(s) 512, 1835, 3177
BoxI GACNNNNGTC 2 cut(s) 2410, 3361
BpiI GAAGAC 2 cut(s) 129, 219
BplI GAGNNNNNCTC 2 cut(s) 625, 657
Bpu14I TTCGAA 3 cut(s) 120, 1465, 1863
BpuEI CTTGAG 5 cut(s) 277, 1293, 2183, 2327, 2675
BpuMI CCSGG 3 cut(s) 102, 2463, 3302
BsaAI YACGTR 1 cut(s) 3272
BsaBI GATNNNNATC 1 cut(s) 2541
BsaHI GRCGYC 3 cut(s) 5, 105, 126
BsaI GGTCTC 1 cut(s) 2307
BsaJI CCNNGG 3 cut(s) 100, 432, 1726
BsaWI WCCGGW 4 cut(s) 74, 234, 684, 965
BsaXI ACNNNNNCTCC 4 cut(s) 657, 687, 837, 867
Bsc4I CCNNNNNNNGG 4 cut(s) 450, 865, 1313, 3307
Bse118I RCCGGY 2 cut(s) 234, 684
Bse1I ACTGG 8 cut(s) 518, 741, 1163, 1841, 2484, 2499, 3114, 3172
Bse21I CCTNAGG 2 cut(s) 1719, 2126
Bse3DI GCAATG 4 cut(s) 201, 372, 870, 888
Bse8I GATNNNNATC 1 cut(s) 2541
BseAI TCCGGA 1 cut(s) 965
BseBI CCWGG 5 cut(s) 451, 1170, 1728, 2923, 2939
BseDI CCNNGG 3 cut(s) 100, 432, 1726
BseGI GGATG 7 cut(s) 349, 675, 855, 1264, 1835, 2454, 3049
BseJI GATNNNNATC 1 cut(s) 2541
BseLI CCNNNNNNNGG 4 cut(s) 450, 865, 1313, 3307
BseMI GCAATG 4 cut(s) 201, 372, 870, 888
BseMII CTCAG 4 cut(s) 1443, 1710, 2122, 3198
BseNI ACTGG 8 cut(s) 518, 741, 1163, 1841, 2484, 2499, 3114, 3172
BseRI GAGGAG 2 cut(s) 378, 641
BseSI GKGCMC 1 cut(s) 242
BseXI GCAGC 3 cut(s) 178, 220, 948
BseYI CCCAGC 1 cut(s) 2707
Bsh1236I CGCG 2 cut(s) 1707, 3335
Bsh1285I CGRYCG 1 cut(s) 2956
BshFI GGCC 7 cut(s) 84, 1048, 1806, 2124, 2461, 3400, 3417
BshNI GGYRCC 2 cut(s) 456, 2728
BshTI ACCGGT 2 cut(s) 234, 684
BsiEI CGRYCG 1 cut(s) 2956
BsiHKAI GWGCWC 3 cut(s) 242, 643, 2706
BsiSI CCGG 9 cut(s) 45, 75, 85, 102, 235, 685, 966, 2462, 3301
BsiWI CGTACG 1 cut(s) 3381
BslI CCNNNNNNNGG 4 cut(s) 450, 865, 1313, 3307
BsmAI GTCTC 5 cut(s) 751, 994, 1149, 2307, 2651
BsmI GAATGC 2 cut(s) 251, 1155
BsnI GGCC 7 cut(s) 84, 1048, 1806, 2124, 2461, 3400, 3417
Bso31I GGTCTC 1 cut(s) 2307
Bsp119I TTCGAA 3 cut(s) 120, 1465, 1863
Bsp1286I GDGCHC 4 cut(s) 242, 643, 774, 2706
Bsp13I TCCGGA 1 cut(s) 965
Bsp1407I TGTACA 1 cut(s) 2570
Bsp143I GATC 8 cut(s) 21, 295, 830, 1065, 1385, 1501, 2289, 2563
Bsp68I TCGCGA 1 cut(s) 1707
BspACI CCGC 4 cut(s) 27, 48, 2325, 3418
BspANI GGCC 7 cut(s) 84, 1048, 1806, 2124, 2461, 3400, 3417
BspCNI CTCAG 4 cut(s) 1444, 1711, 2121, 3199
BspEI TCCGGA 1 cut(s) 965
BspFNI CGCG 2 cut(s) 1707, 3335
BspHI TCATGA 5 cut(s) 1219, 1504, 2347, 3148, 3455
BspLI GGNNCC 9 cut(s) 458, 537, 1362, 1725, 2074, 2556, 2730, 2931, 3399
BspOI GCTAGC 1 cut(s) 936
BspPI GGATC 3 cut(s) 29, 838, 1393
BspQI GCTCTTC 2 cut(s) 86, 2039
BspT104I TTCGAA 3 cut(s) 120, 1465, 1863
BspT107I GGYRCC 2 cut(s) 456, 2728
BspTI CTTAAG 2 cut(s) 1268, 2177
BspTNI GGTCTC 1 cut(s) 2307
BsrDI GCAATG 4 cut(s) 201, 372, 870, 888
BsrFI RCCGGY 2 cut(s) 234, 684
BsrGI TGTACA 1 cut(s) 2570
BsrI ACTGG 8 cut(s) 518, 741, 1163, 1841, 2484, 2499, 3114, 3172
BssAI RCCGGY 2 cut(s) 234, 684
BssECI CCNNGG 3 cut(s) 100, 432, 1726
BssMI GATC 8 cut(s) 21, 295, 830, 1065, 1385, 1501, 2289, 2563
BssNI GRCGYC 3 cut(s) 5, 105, 126
BssT1I CCWWGG 1 cut(s) 432
Bst2UI CCWGG 5 cut(s) 451, 1170, 1728, 2923, 2939
Bst4CI ACNGT 8 cut(s) 1746, 2354, 2362, 2409, 2954, 3193, 3369, 3381
Bst6I CTCTTC 6 cut(s) 86, 451, 781, 2039, 2478, 2633
BstACI GRCGYC 3 cut(s) 5, 105, 126
BstAFI CTTAAG 2 cut(s) 1268, 2177
BstAPI GCANNNNNTGC 1 cut(s) 1675
BstAUI TGTACA 1 cut(s) 2570
BstBAI YACGTR 1 cut(s) 3272
BstBI TTCGAA 3 cut(s) 120, 1465, 1863
BstC8I GCNNGC 6 cut(s) 639, 934, 992, 1287, 2881, 2983
BstENI CCTNNNNNAGG 2 cut(s) 448, 1311
BstF5I GGATG 7 cut(s) 349, 675, 855, 1264, 1835, 2454, 3049
BstFNI CGCG 2 cut(s) 1707, 3335
BstHHI GCGC 2 cut(s) 272, 3337
BstKTI GATC 8 cut(s) 24, 298, 833, 1068, 1388, 1504, 2292, 2566
BstMAI GTCTC 5 cut(s) 751, 994, 1149, 2307, 2651
BstMBI GATC 8 cut(s) 21, 295, 830, 1065, 1385, 1501, 2289, 2563
BstMCI CGRYCG 1 cut(s) 2956
BstMWI GCNNNNNNNGC 7 cut(s) 102, 137, 899, 1675, 1972, 2701, 2964
BstNI CCWGG 5 cut(s) 451, 1170, 1728, 2923, 2939
BstNSI RCATGY 3 cut(s) 817, 2063, 3362
BstPAI GACNNNNGTC 2 cut(s) 2410, 3361
BstSCI CCNGG 8 cut(s) 100, 449, 1168, 1726, 2461, 2921, 2937, 3300
BstSFI CTRYAG 2 cut(s) 386, 3388
BstSLI GKGCMC 1 cut(s) 242
BstUI CGCG 2 cut(s) 1707, 3335
BstV1I GCAGC 3 cut(s) 178, 220, 948
BstV2I GAAGAC 2 cut(s) 129, 219
BstX2I RGATCY 1 cut(s) 1385
BstYI RGATCY 1 cut(s) 1385
Bsu36I CCTNAGG 2 cut(s) 1719, 2126
BsuRI GGCC 7 cut(s) 84, 1048, 1806, 2124, 2461, 3400, 3417
BtsCI GGATG 7 cut(s) 349, 675, 855, 1264, 1835, 2454, 3049
BtsIMutI CAGTG 5 cut(s) 2359, 2492, 2883, 3121, 3365
BtuMI TCGCGA 1 cut(s) 1707
Cac8I GCNNGC 6 cut(s) 639, 934, 992, 1287, 2881, 2983
CciI TCATGA 5 cut(s) 1219, 1504, 2347, 3148, 3455
CfoI GCGC 2 cut(s) 272, 3337
Cfr10I RCCGGY 2 cut(s) 234, 684
Cfr13I GGNCC 4 cut(s) 1046, 1723, 1804, 3398
CseI GACGC 1 cut(s) 94
CspAI ACCGGT 2 cut(s) 234, 684
CspCI CAANNNNNGTGG 6 cut(s) 58, 93, 1585, 1620, 2496, 2531
DpnI GATC 8 cut(s) 23, 297, 832, 1067, 1387, 1503, 2291, 2565
DpnII GATC 8 cut(s) 21, 295, 830, 1065, 1385, 1501, 2289, 2563
DraI TTTAAA 3 cut(s) 678, 1989, 2782
DrdI GACNNNNNNGTC 2 cut(s) 1302, 1614
DseDI GACNNNNNNGTC 2 cut(s) 1302, 1614
EaeI YGGCCR 3 cut(s) 82, 2459, 3415
Eam1104I CTCTTC 6 cut(s) 86, 451, 781, 2039, 2478, 2633
EarI CTCTTC 6 cut(s) 86, 451, 781, 2039, 2478, 2633
Ecl136II GAGCTC 2 cut(s) 641, 2704
Eco130I CCWWGG 1 cut(s) 432
Eco24I GRGCYC 3 cut(s) 643, 774, 2706
Eco31I GGTCTC 1 cut(s) 2307
Eco32I GATATC 1 cut(s) 1015
Eco47I GGWCC 1 cut(s) 1723
Eco53kI GAGCTC 2 cut(s) 641, 2704
Eco57I CTGAAG 1 cut(s) 1071
Eco81I CCTNAGG 2 cut(s) 1719, 2126
EcoICRI GAGCTC 2 cut(s) 641, 2704
EcoNI CCTNNNNNAGG 2 cut(s) 448, 1311
EcoO109I RGGNCCY 2 cut(s) 1723, 1804
EcoRII CCWGG 5 cut(s) 449, 1168, 1726, 2921, 2937
EcoRV GATATC 1 cut(s) 1015
EcoT14I CCWWGG 1 cut(s) 432
EcoT22I ATGCAT 2 cut(s) 253, 1671
EcoT38I GRGCYC 3 cut(s) 643, 774, 2706
ErhI CCWWGG 1 cut(s) 432
FauI CCCGC 1 cut(s) 41
FauNDI CATATG 2 cut(s) 244, 3237
FbaI TGATCA 1 cut(s) 2289
FblI GTMKAC 2 cut(s) 1650, 3173
Fnu4HI GCNGC 5 cut(s) 27, 192, 209, 937, 3418
FokI GGATG 7 cut(s) 356, 682, 842, 1271, 1822, 2461, 3036
FriOI GRGCYC 3 cut(s) 643, 774, 2706
Fsp4HI GCNGC 5 cut(s) 27, 192, 209, 937, 3418
FspBI CTAG 8 cut(s) 585, 933, 1058, 1194, 1740, 2024, 2385, 2403
GlaI GCGC 2 cut(s) 271, 3336
GluI GCNGC 5 cut(s) 27, 192, 209, 937, 3418
GsaI CCCAGC 1 cut(s) 2711
HaeIII GGCC 7 cut(s) 84, 1048, 1806, 2124, 2461, 3400, 3417
HapII CCGG 9 cut(s) 45, 75, 85, 102, 235, 685, 966, 2462, 3301
HgaI GACGC 1 cut(s) 94
HhaI GCGC 2 cut(s) 272, 3337
Hin1I GRCGYC 3 cut(s) 5, 105, 126
Hin6I GCGC 2 cut(s) 270, 3335
HinP1I GCGC 2 cut(s) 270, 3335
HincII GTYRAC 3 cut(s) 1662, 2415, 3174
HindII GTYRAC 3 cut(s) 1662, 2415, 3174
HindIII AAGCTT 6 cut(s) 1271, 2273, 2336, 2521, 2830, 3064
HpaI GTTAAC 1 cut(s) 1662
HpaII CCGG 9 cut(s) 45, 75, 85, 102, 235, 685, 966, 2462, 3301
HphI GGTGA 4 cut(s) 290, 1707, 3316, 3369
Hpy188I TCNGA 9 cut(s) 266, 544, 949, 1012, 1126, 2140, 2536, 2551, 3105
Hpy99I CGWCG 5 cut(s) 10, 131, 146, 149, 155
HpyCH4III ACNGT 8 cut(s) 1746, 2354, 2362, 2409, 2954, 3193, 3369, 3381
HpyF10VI GCNNNNNNNGC 7 cut(s) 102, 137, 899, 1675, 1972, 2701, 2964
Hsp92I GRCGYC 3 cut(s) 5, 105, 126
HspAI GCGC 2 cut(s) 270, 3335
Kpn2I TCCGGA 1 cut(s) 965
KpnI GGTACC 1 cut(s) 2732
Ksp22I TGATCA 1 cut(s) 2289
KspAI GTTAAC 1 cut(s) 1662
Kzo9I GATC 8 cut(s) 21, 295, 830, 1065, 1385, 1501, 2289, 2563
LguI GCTCTTC 2 cut(s) 86, 2039
LmnI GCTCC 4 cut(s) 382, 1126, 1324, 2709
Lsp1109I GCAGC 3 cut(s) 178, 220, 948
LweI GCATC 5 cut(s) 238, 1678, 1747, 1875, 3383
MaeI CTAG 8 cut(s) 585, 933, 1058, 1194, 1740, 2024, 2385, 2403
MaeIII GTNAC 6 cut(s) 158, 405, 1316, 2606, 3363, 3407
MalI GATC 8 cut(s) 23, 297, 832, 1067, 1387, 1503, 2291, 2565
MboI GATC 8 cut(s) 21, 295, 830, 1065, 1385, 1501, 2289, 2563
MfeI CAATTG 1 cut(s) 1673
MflI RGATCY 1 cut(s) 1385
MhlI GDGCHC 4 cut(s) 242, 643, 774, 2706
MlyI GAGTC 6 cut(s) 171, 413, 701, 2464, 2650, 2653
MmeI TCCRAC 4 cut(s) 706, 823, 1585, 2697
Mph1103I ATGCAT 2 cut(s) 253, 1671
MroI TCCGGA 1 cut(s) 965
MroXI GAANNNNTTC 3 cut(s) 1902, 1944, 2694
MslI CAYNNNNRTG 5 cut(s) 1257, 1755, 2118, 2352, 3054
MspCI CTTAAG 2 cut(s) 1268, 2177
MspI CCGG 9 cut(s) 45, 75, 85, 102, 235, 685, 966, 2462, 3301
MspR9I CCNGG 8 cut(s) 102, 451, 1170, 1728, 2463, 2923, 2939, 3302
MunI CAATTG 1 cut(s) 1673
Mva1269I GAATGC 2 cut(s) 251, 1155
MvaI CCWGG 5 cut(s) 451, 1170, 1728, 2923, 2939
MvnI CGCG 2 cut(s) 1707, 3335
MwoI GCNNNNNNNGC 7 cut(s) 102, 137, 899, 1675, 1972, 2701, 2964
NciI CCSGG 3 cut(s) 102, 2463, 3302
NdeI CATATG 2 cut(s) 244, 3237
NdeII GATC 8 cut(s) 21, 295, 830, 1065, 1385, 1501, 2289, 2563
NheI GCTAGC 1 cut(s) 932
NlaIV GGNNCC 9 cut(s) 458, 537, 1362, 1725, 2074, 2556, 2730, 2931, 3399
NmuCI GTSAC 3 cut(s) 158, 405, 3363
NruI TCGCGA 1 cut(s) 1707
NsiI ATGCAT 2 cut(s) 253, 1671
NspI RCATGY 3 cut(s) 817, 2063, 3362
NspV TTCGAA 3 cut(s) 120, 1465, 1863
PagI TCATGA 5 cut(s) 1219, 1504, 2347, 3148, 3455
PciI ACATGT 3 cut(s) 813, 2059, 3358
PciSI GCTCTTC 2 cut(s) 86, 2039
PcsI WCGNNNNNNNCGW 5 cut(s) 132, 141, 150, 1078, 2898
PctI GAATGC 2 cut(s) 251, 1155
PdmI GAANNNNTTC 3 cut(s) 1902, 1944, 2694
Pfl23II CGTACG 1 cut(s) 3381
PflMI CCANNNNNTGG 1 cut(s) 865
PfoI TCCNGGA 1 cut(s) 1168
PinAI ACCGGT 2 cut(s) 234, 684
PkrI GCNGC 5 cut(s) 28, 193, 210, 938, 3419
PleI GAGTC 6 cut(s) 171, 412, 700, 2464, 2649, 2653
PpsI GAGTC 6 cut(s) 171, 412, 700, 2464, 2649, 2653
Ppu21I YACGTR 1 cut(s) 3272
PpuMI RGGWCCY 1 cut(s) 1723
PscI ACATGT 3 cut(s) 813, 2059, 3358
PshAI GACNNNNGTC 2 cut(s) 2410, 3361
PshBI ATTAAT 2 cut(s) 1644, 2190
PsiI TTATAA 2 cut(s) 906, 1184
Psp124BI GAGCTC 2 cut(s) 643, 2706
Psp1406I AACGTT 3 cut(s) 1513, 1767, 2849
Psp5II RGGWCCY 1 cut(s) 1723
Psp6I CCWGG 5 cut(s) 449, 1168, 1726, 2921, 2937
PspFI CCCAGC 1 cut(s) 2707
PspGI CCWGG 5 cut(s) 449, 1168, 1726, 2921, 2937
PspLI CGTACG 1 cut(s) 3381
PspN4I GGNNCC 9 cut(s) 458, 537, 1362, 1725, 2074, 2556, 2730, 2931, 3399
PspPI GGNCC 4 cut(s) 1046, 1723, 1804, 3398
PspPPI RGGWCCY 1 cut(s) 1723
PsuI RGATCY 1 cut(s) 1385
RruI TCGCGA 1 cut(s) 1707
RseI CAYNNNNRTG 5 cut(s) 1257, 1755, 2118, 2352, 3054
SacI GAGCTC 2 cut(s) 643, 2706
SalI GTCGAC 1 cut(s) 3172
SapI GCTCTTC 2 cut(s) 86, 2039
SatI GCNGC 5 cut(s) 27, 192, 209, 937, 3418
Sau3AI GATC 8 cut(s) 21, 295, 830, 1065, 1385, 1501, 2289, 2563
Sau96I GGNCC 4 cut(s) 1046, 1723, 1804, 3398
ScaI AGTACT 1 cut(s) 3166
SchI GAGTC 6 cut(s) 171, 413, 701, 2464, 2650, 2653
ScrFI CCNGG 8 cut(s) 102, 451, 1170, 1728, 2463, 2923, 2939, 3302
SduI GDGCHC 4 cut(s) 242, 643, 774, 2706
SfaNI GCATC 5 cut(s) 238, 1678, 1747, 1875, 3383
SfcI CTRYAG 2 cut(s) 386, 3388
SfuI TTCGAA 3 cut(s) 120, 1465, 1863
SinI GGWCC 1 cut(s) 1723
SmiMI CAYNNNNRTG 5 cut(s) 1257, 1755, 2118, 2352, 3054
SmlI CTYRAG 7 cut(s) 256, 1268, 1308, 2162, 2177, 2306, 2654
SmoI CTYRAG 7 cut(s) 256, 1268, 1308, 2162, 2177, 2306, 2654
SsiI CCGC 4 cut(s) 27, 48, 2325, 3418
SspI AATATT 1 cut(s) 1962
SspMI CTAG 8 cut(s) 585, 933, 1058, 1194, 1740, 2024, 2385, 2403
SstI GAGCTC 2 cut(s) 643, 2706
StyD4I CCNGG 8 cut(s) 100, 449, 1168, 1726, 2461, 2921, 2937, 3300
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 8 cut(s) 1746, 2354, 2362, 2409, 2954, 3193, 3369, 3381
TaqI TCGA 9 cut(s) 8, 120, 165, 1465, 1863, 2688, 2901, 3173, 3427
TaqII GACCGA 1 cut(s) 2927
TatI WGTACW 2 cut(s) 2570, 3164
TauI GCSGC 2 cut(s) 29, 3420
TscAI CASTG 5 cut(s) 2359, 2499, 2890, 3121, 3372
TseFI GTSAC 3 cut(s) 158, 405, 3363
TseI GCWGC 3 cut(s) 191, 208, 936
Tsp45I GTSAC 3 cut(s) 158, 405, 3363
TspGWI ACGGA 1 cut(s) 2056
TspRI CASTG 5 cut(s) 2359, 2499, 2890, 3121, 3372
Van91I CCANNNNNTGG 1 cut(s) 865
Vha464I CTTAAG 2 cut(s) 1268, 2177
VneI GTGCAC 1 cut(s) 238
VpaK11BI GGWCC 1 cut(s) 1723
VspI ATTAAT 2 cut(s) 1644, 2190
XagI CCTNNNNNAGG 2 cut(s) 448, 1311
XapI RAATTY 6 cut(s) 923, 1461, 1954, 2097, 2764, 2794
XceI RCATGY 3 cut(s) 817, 2063, 3362
XcmI CCANNNNNNNNNTGG 4 cut(s) 866, 2221, 2439, 2491
XmiI GTMKAC 2 cut(s) 1650, 3173
XmnI GAANNNNTTC 3 cut(s) 1902, 1944, 2694
XspI CTAG 8 cut(s) 585, 933, 1058, 1194, 1740, 2024, 2385, 2403
ZraI GACGTC 1 cut(s) 127
ZrmI AGTACT 1 cut(s) 3166
Zsp2I ATGCAT 2 cut(s) 253, 1671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.