Rh6CG296200

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0000654
Physical Location & Seq
Reverse (-)
47725423 .. 47726646
1224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG296200.1

Sequence Viewer

Length: 1059 bp
ATGCTCGACACTCTTTTCTTTGGAGACTGCACCAAGCTCAAAATGCTTCCAGAGCTCCCACCAAGTTTAAGTACTTTGCATGCAGAAGGTTGTACATCGTTGAGAAAAGTTACGAACTTACCAAACTTTTTCATATCCCTACAACCATATCTTACGGATTGCAAAGAATTAGCTGAAGTTCAAAGCTTATTCACTATAAAACCATTGAGAAGCATCGACATACAGATGATAAAATATATGGGTCTGTACAATTTGGAATCCACTGAAAGCATTGAAGTCGAAATGAGCAATCACCTGACAGATACAAACAAGACAGGTCCACTCCAGGGACTCTATGAATGTGGAATATTTAGCATTTTCCTTCATGGAAGCACAATTCCTGACTGCTACACCTACAGAAGCATGGGTGATTCAGTGTTGTCTATAACTGTTCCTTCCCATCTTAAGCTTAAGATCCGAGGCTTAAATGTATGTATTCTATATGCACTAGGCCCATGCCAGCACCTTAAAGTCAGTAATGACACGAAGGGTCTTATGTGGACATACTGCCCCGTCACTGTTGGTGTTCCTAAAGAGGATGGACTGATGCTATGGCTAAGTCATTGGCTGTTTGAGAACCATGAGTTGGAGGCTGGGGATGAATTACGTGTCTCGGTGCATAATGGAGCTATATTCTTTGACCCTCTTGGTCATCGAGACGTTCTTCCAGCAAAGGAATTTGGAATCCAACTTGTGTATGAATCGGAAAATAAGGACGTCCGATCCAAAAGTGAAGATATTACGGTACAACTTGAAGCGCCTTATTGGAGTTACAATATTGTTACTGGGAATGGATCATTGTCAGCATCAAAGTATCAAATGTGGACAGGCAAATACTTTCTTTGCAATTATATTTACAATATGCGTCAGATTCATTTCAGAAATTGCGAGGAGAATCCAGCTCATCTTGATTTTTCATATGAGCCCAAAGATCGTTTGAGTGGATATTGCCGTCATTTATTTGAGCACACTCACTTTCACAAGAAAAAATTTGAGTTTGTATTGAATCTCTTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

352

Amino Acids

40.44

Weight (kDa)

6.01

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 121 - 249 7.8e-06 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 759
AccB7I CCANNNNNTGG 1 cut(s) 625
AclWI GGATC 3 cut(s) 448, 756, 841
AcsI RAATTY 2 cut(s) 716, 1028
AcuI CTGAAG 1 cut(s) 195
AcyI GRCGYC 1 cut(s) 756
AfaI GTAC 4 cut(s) 73, 94, 248, 786
AfiI CCNNNNNNNGG 2 cut(s) 326, 625
AflII CTTAAG 2 cut(s) 443, 449
AflIII ACRYGT 1 cut(s) 646
AgsI TTSAA 4 cut(s) 182, 275, 794, 1045
AjnI CCWGG 1 cut(s) 324
AjuI GAANNNNNNNTTGG 2 cut(s) 608, 640
AluBI AGCT 7 cut(s) 37, 55, 173, 186, 448, 668, 941
AluI AGCT 7 cut(s) 37, 55, 173, 186, 448, 668, 941
Alw21I GWGCWC 2 cut(s) 57, 1008
Alw26I GTCTC 3 cut(s) 18, 655, 690
AlwI GGATC 3 cut(s) 448, 756, 841
AoxI GGCC 1 cut(s) 490
ApoI RAATTY 2 cut(s) 716, 1028
AspLEI GCGC 1 cut(s) 799
AspS9I GGNCC 2 cut(s) 317, 491
AsuHPI GGTGA 2 cut(s) 284, 419
AvaII GGWCC 1 cut(s) 317
BanII GRGCYC 2 cut(s) 57, 966
Bbv12I GWGCWC 2 cut(s) 57, 1008
BccI CCATC 2 cut(s) 447, 572
BceAI ACGGC 1 cut(s) 975
BcgI CGANNNNNNTGC 2 cut(s) 259, 293
BciT130I CCWGG 1 cut(s) 326
BcoDI GTCTC 3 cut(s) 18, 655, 690
BfaI CTAG 1 cut(s) 488
BfmI CTRYAG 1 cut(s) 394
BfoI RGCGCY 1 cut(s) 800
BfrI CTTAAG 2 cut(s) 443, 449
BmcAI AGTACT 1 cut(s) 73
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 317
BmgT120I GGNCC 2 cut(s) 317, 491
BmrFI CCNGG 1 cut(s) 326
BmrI ACTGGG 1 cut(s) 834
BmsI GCATC 3 cut(s) 222, 576, 854
BmuI ACTGGG 1 cut(s) 834
BpmI CTGGAG 1 cut(s) 308
BsaAI YACGTR 1 cut(s) 647
BsaHI GRCGYC 1 cut(s) 756
BsaJI CCNNGG 2 cut(s) 325, 457
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 625
Bse1I ACTGG 1 cut(s) 829
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 2 cut(s) 325, 457
BseGI GGATG 2 cut(s) 583, 643
BseLI CCNNNNNNNGG 2 cut(s) 326, 625
BseNI ACTGG 1 cut(s) 829
BseRI GAGGAG 1 cut(s) 944
BseYI CCCAGC 1 cut(s) 632
BsgI GTGCAG 1 cut(s) 13
BshFI GGCC 1 cut(s) 492
BsiHKAI GWGCWC 2 cut(s) 57, 1008
BslFI GGGAC 1 cut(s) 342
BslI CCNNNNNNNGG 2 cut(s) 326, 625
BsmAI GTCTC 3 cut(s) 18, 655, 690
BsmBI CGTCTC 1 cut(s) 690
BsmFI GGGAC 1 cut(s) 342
BsnI GGCC 1 cut(s) 492
Bsp1286I GDGCHC 3 cut(s) 57, 966, 1008
Bsp1407I TGTACA 2 cut(s) 92, 246
Bsp143I GATC 4 cut(s) 453, 761, 833, 970
BspANI GGCC 1 cut(s) 492
BspPI GGATC 3 cut(s) 448, 756, 841
BspTI CTTAAG 2 cut(s) 443, 449
BsrGI TGTACA 2 cut(s) 92, 246
BsrI ACTGG 1 cut(s) 829
BssECI CCNNGG 2 cut(s) 325, 457
BssMI GATC 4 cut(s) 453, 761, 833, 970
BssNI GRCGYC 1 cut(s) 756
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 3 cut(s) 430, 559, 784
BstACI GRCGYC 1 cut(s) 756
BstAFI CTTAAG 2 cut(s) 443, 449
BstAUI TGTACA 2 cut(s) 92, 246
BstBAI YACGTR 1 cut(s) 647
BstC8I GCNNGC 2 cut(s) 81, 500
BstDEI CTNAG 1 cut(s) 596
BstF5I GGATG 2 cut(s) 583, 643
BstH2I RGCGCY 1 cut(s) 800
BstHHI GCGC 1 cut(s) 799
BstKTI GATC 4 cut(s) 456, 764, 836, 973
BstMAI GTCTC 3 cut(s) 18, 655, 690
BstMBI GATC 4 cut(s) 453, 761, 833, 970
BstMWI GCNNNNNNNGC 2 cut(s) 43, 52
BstNI CCWGG 1 cut(s) 326
BstNSI RCATGY 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 324
BstSFI CTRYAG 1 cut(s) 394
BstX2I RGATCY 1 cut(s) 453
BstYI RGATCY 1 cut(s) 453
BsuRI GGCC 1 cut(s) 492
BtsCI GGATG 2 cut(s) 583, 643
BtsIMutI CAGTG 3 cut(s) 261, 420, 555
Cac8I GCNNGC 2 cut(s) 81, 500
CfoI GCGC 1 cut(s) 799
Cfr13I GGNCC 2 cut(s) 317, 491
CseI GACGC 1 cut(s) 893
Csp6I GTAC 4 cut(s) 72, 93, 247, 785
CviAII CATG 5 cut(s) 80, 365, 403, 495, 620
CviQI GTAC 4 cut(s) 72, 93, 247, 785
DdeI CTNAG 1 cut(s) 596
DpnI GATC 4 cut(s) 455, 763, 835, 972
DpnII GATC 4 cut(s) 453, 761, 833, 970
Ecl136II GAGCTC 1 cut(s) 55
Eco24I GRGCYC 2 cut(s) 57, 966
Eco47I GGWCC 1 cut(s) 317
Eco53kI GAGCTC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 195
EcoICRI GAGCTC 1 cut(s) 55
EcoRII CCWGG 1 cut(s) 324
EcoT38I GRGCYC 2 cut(s) 57, 966
Esp3I CGTCTC 1 cut(s) 690
FaeI CATG 5 cut(s) 83, 368, 406, 498, 623
FaqI GGGAC 1 cut(s) 342
FatI CATG 5 cut(s) 79, 364, 402, 494, 619
FauNDI CATATG 1 cut(s) 958
FokI GGATG 2 cut(s) 590, 650
FriOI GRGCYC 2 cut(s) 57, 966
FspBI CTAG 1 cut(s) 488
GlaI GCGC 1 cut(s) 798
GsaI CCCAGC 1 cut(s) 636
GsuI CTGGAG 1 cut(s) 308
HaeII RGCGCY 1 cut(s) 800
HaeIII GGCC 1 cut(s) 492
HgaI GACGC 1 cut(s) 893
HhaI GCGC 1 cut(s) 799
Hin1I GRCGYC 1 cut(s) 756
Hin1II CATG 5 cut(s) 83, 368, 406, 498, 623
Hin6I GCGC 1 cut(s) 797
HinP1I GCGC 1 cut(s) 797
HindIII AAGCTT 2 cut(s) 184, 446
HinfI GANTC 8 cut(s) 257, 330, 410, 723, 740, 910, 934, 1045
HphI GGTGA 2 cut(s) 284, 419
Hpy166II GTNNAC 3 cut(s) 320, 540, 864
Hpy188I TCNGA 5 cut(s) 458, 745, 761, 909, 920
Hpy188III TCNNGA 4 cut(s) 50, 380, 695, 947
Hpy8I GTNNAC 3 cut(s) 320, 540, 864
HpyAV CCTTC 4 cut(s) 80, 371, 444, 520
HpyCH4III ACNGT 3 cut(s) 430, 559, 784
HpyCH4IV ACGT 3 cut(s) 646, 699, 756
HpyCH4V TGCA 7 cut(s) 30, 79, 83, 162, 485, 658, 885
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 52
HpyF3I CTNAG 1 cut(s) 596
HpySE526I ACGT 3 cut(s) 646, 699, 756
Hsp92I GRCGYC 1 cut(s) 756
Hsp92II CATG 5 cut(s) 83, 368, 406, 498, 623
HspAI GCGC 1 cut(s) 797
Kzo9I GATC 4 cut(s) 453, 761, 833, 970
LmnI GCTCC 2 cut(s) 60, 665
LweI GCATC 3 cut(s) 222, 576, 854
MaeI CTAG 1 cut(s) 488
MaeII ACGT 3 cut(s) 646, 699, 756
MaeIII GTNAC 4 cut(s) 109, 553, 809, 820
MalI GATC 4 cut(s) 455, 763, 835, 972
MboI GATC 4 cut(s) 453, 761, 833, 970
MboII GAAGA 2 cut(s) 695, 785
MflI RGATCY 1 cut(s) 453
MhlI GDGCHC 3 cut(s) 57, 966, 1008
MluCI AATT 8 cut(s) 167, 250, 375, 641, 716, 886, 922, 1028
MlyI GAGTC 1 cut(s) 324
MmeI TCCRAC 2 cut(s) 606, 751
MnlI CCTC 5 cut(s) 452, 568, 622, 693, 922
MseI TTAA 5 cut(s) 68, 444, 450, 464, 507
MslI CAYNNNNRTG 1 cut(s) 224
MspCI CTTAAG 2 cut(s) 443, 449
MspR9I CCNGG 1 cut(s) 326
MvaI CCWGG 1 cut(s) 326
MwoI GCNNNNNNNGC 2 cut(s) 43, 52
NdeI CATATG 1 cut(s) 958
NdeII GATC 4 cut(s) 453, 761, 833, 970
NlaIII CATG 5 cut(s) 83, 368, 406, 498, 623
NmuCI GTSAC 1 cut(s) 553
NspI RCATGY 1 cut(s) 83
PaeI GCATGC 1 cut(s) 83
PfeI GAWTC 7 cut(s) 257, 410, 723, 740, 910, 934, 1045
PflMI CCANNNNNTGG 1 cut(s) 625
PleI GAGTC 1 cut(s) 324
PpsI GAGTC 1 cut(s) 324
Ppu21I YACGTR 1 cut(s) 647
Psp124BI GAGCTC 1 cut(s) 57
Psp6I CCWGG 1 cut(s) 324
PspFI CCCAGC 1 cut(s) 632
PspGI CCWGG 1 cut(s) 324
PspPI GGNCC 2 cut(s) 317, 491
PsuI RGATCY 1 cut(s) 453
RsaI GTAC 4 cut(s) 73, 94, 248, 786
RsaNI GTAC 4 cut(s) 72, 93, 247, 785
RseI CAYNNNNRTG 1 cut(s) 224
SacI GAGCTC 1 cut(s) 57
SaqAI TTAA 5 cut(s) 68, 444, 450, 464, 507
Sau3AI GATC 4 cut(s) 453, 761, 833, 970
Sau96I GGNCC 2 cut(s) 317, 491
ScaI AGTACT 1 cut(s) 73
SchI GAGTC 1 cut(s) 324
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 3 cut(s) 57, 966, 1008
SfaNI GCATC 3 cut(s) 222, 576, 854
SfcI CTRYAG 1 cut(s) 394
SinI GGWCC 1 cut(s) 317
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 2 cut(s) 443, 449
SmoI CTYRAG 2 cut(s) 443, 449
SphI GCATGC 1 cut(s) 83
Sse9I AATT 8 cut(s) 167, 250, 375, 641, 716, 886, 922, 1028
SspI AATATT 2 cut(s) 348, 817
SspMI CTAG 1 cut(s) 488
SstI GAGCTC 1 cut(s) 57
StyD4I CCNGG 1 cut(s) 324
TaaI ACNGT 3 cut(s) 430, 559, 784
TaiI ACGT 3 cut(s) 649, 702, 759
TaqI TCGA 4 cut(s) 6, 216, 279, 694
TasI AATT 8 cut(s) 167, 250, 375, 641, 716, 886, 922, 1028
TatI WGTACW 3 cut(s) 71, 92, 246
TfiI GAWTC 7 cut(s) 257, 410, 723, 740, 910, 934, 1045
Tru1I TTAA 5 cut(s) 68, 444, 450, 464, 507
Tru9I TTAA 5 cut(s) 68, 444, 450, 464, 507
TscAI CASTG 3 cut(s) 268, 420, 562
TseFI GTSAC 1 cut(s) 553
Tsp45I GTSAC 1 cut(s) 553
TspDTI ATGAA 7 cut(s) 121, 351, 353, 654, 753, 902, 945
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 3 cut(s) 268, 420, 562
Van91I CCANNNNNTGG 1 cut(s) 625
Vha464I CTTAAG 2 cut(s) 443, 449
VpaK11BI GGWCC 1 cut(s) 317
XapI RAATTY 2 cut(s) 716, 1028
XceI RCATGY 1 cut(s) 83
XspI CTAG 1 cut(s) 488
ZraI GACGTC 1 cut(s) 757
ZrmI AGTACT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.