Rmu_sc0027178.1_g000001

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0027178.1
OG0000654
Physical Location & Seq
Reverse (-)
2 .. 699
698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0027178.1_g000001.1.cds

Sequence Viewer

Length: 698 bp
atgaaacagggactctatgaatgtggaatatttagcattttcctgcatggaagcacaattcctgcagactgctacacctacagaagcatgggtgattcagtgttgtctatcactgttccttcccatcttaagcttaagatccgaggcttaaatgtatgtattctatatgcattaggcccatgccaccaccttaaagtcagtaatgacacgaagggtcttatgtggacatactgcccagtcactgcaggtgttcctaaagaggatggactgatgctatggctaagccattggctgtttgagaaccatgagttggaggctggggatgaattacgtgtctcggtgcataatggagctatattctttgaccctcttggtcgtcaagacgttcttccagcaaaggaatttggaatccaacttgtgtatgaatcggaaaataaggacgtccgatccaaaagtgaagatcttacggtacaacatgaagcgccttattggagttacaatattgttactgggaatggatcattgtcagcatcaaagtatcaaatgtggacaggcaaatactttctttgcaattatgttcaccatatgcgtcagcttcatttcagaaattgcgaggcaaatccagctcatcttgatttttcatatgagcccaaagatcgtttgagtggatattgccgtcatttatttgatcacactca
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

232

Amino Acids

26.57

Weight (kDa)

6.38

Isoelectric Point (pI)

29.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 236
AatII GACGTC 1 cut(s) 444
Acc36I ACCTGC 1 cut(s) 236
AccB7I CCANNNNNTGG 1 cut(s) 310
AclWI GGATC 3 cut(s) 133, 441, 526
AcsI RAATTY 1 cut(s) 401
AcyI GRCGYC 1 cut(s) 441
AfaI GTAC 1 cut(s) 471
AfiI CCNNNNNNNGG 1 cut(s) 310
AflII CTTAAG 2 cut(s) 128, 134
AflIII ACRYGT 1 cut(s) 331
AjuI GAANNNNNNNTTGG 2 cut(s) 293, 325
AluBI AGCT 4 cut(s) 133, 353, 595, 626
AluI AGCT 4 cut(s) 133, 353, 595, 626
Alw26I GTCTC 1 cut(s) 340
AlwI GGATC 3 cut(s) 133, 441, 526
AlwNI CAGNNNCTG 1 cut(s) 242
AoxI GGCC 1 cut(s) 175
ApoI RAATTY 1 cut(s) 401
AspLEI GCGC 1 cut(s) 484
AspS9I GGNCC 1 cut(s) 176
AsuHPI GGTGA 2 cut(s) 104, 572
BanII GRGCYC 1 cut(s) 651
BccI CCATC 2 cut(s) 132, 257
BceAI ACGGC 1 cut(s) 660
BclI TGATCA 1 cut(s) 688
BcoDI GTCTC 1 cut(s) 340
BfmI CTRYAG 3 cut(s) 63, 79, 243
BfoI RGCGCY 1 cut(s) 485
BfrI CTTAAG 2 cut(s) 128, 134
BfuAI ACCTGC 1 cut(s) 236
BglII AGATCT 1 cut(s) 460
BlpI GCTNAGC 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 176
BmrI ACTGGG 2 cut(s) 230, 519
BmsI GCATC 2 cut(s) 261, 539
BmuI ACTGGG 2 cut(s) 230, 519
Bpu1102I GCTNAGC 1 cut(s) 281
BsaAI YACGTR 1 cut(s) 332
BsaHI GRCGYC 1 cut(s) 441
BsaJI CCNNGG 1 cut(s) 142
Bsc4I CCNNNNNNNGG 1 cut(s) 310
Bse1I ACTGG 2 cut(s) 236, 514
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 2 cut(s) 268, 328
BseLI CCNNNNNNNGG 1 cut(s) 310
BseNI ACTGG 2 cut(s) 236, 514
BseYI CCCAGC 1 cut(s) 317
BshFI GGCC 1 cut(s) 177
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 1 cut(s) 310
BsmAI GTCTC 1 cut(s) 340
BsmFI GGGAC 1 cut(s) 24
BsnI GGCC 1 cut(s) 177
Bsp1286I GDGCHC 1 cut(s) 651
Bsp143I GATC 6 cut(s) 138, 446, 460, 518, 655, 688
Bsp1720I GCTNAGC 1 cut(s) 281
BspANI GGCC 1 cut(s) 177
BspMAI CTGCAG 2 cut(s) 67, 247
BspMI ACCTGC 1 cut(s) 236
BspPI GGATC 3 cut(s) 133, 441, 526
BspTI CTTAAG 2 cut(s) 128, 134
BsrI ACTGG 2 cut(s) 236, 514
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 6 cut(s) 138, 446, 460, 518, 655, 688
BssNI GRCGYC 1 cut(s) 441
Bst4CI ACNGT 2 cut(s) 115, 469
BstACI GRCGYC 1 cut(s) 441
BstAFI CTTAAG 2 cut(s) 128, 134
BstBAI YACGTR 1 cut(s) 332
BstDEI CTNAG 1 cut(s) 281
BstF5I GGATG 2 cut(s) 268, 328
BstH2I RGCGCY 1 cut(s) 485
BstHHI GCGC 1 cut(s) 484
BstKTI GATC 6 cut(s) 141, 449, 463, 521, 658, 691
BstMAI GTCTC 1 cut(s) 340
BstMBI GATC 6 cut(s) 138, 446, 460, 518, 655, 688
BstMWI GCNNNNNNNGC 1 cut(s) 623
BstSFI CTRYAG 3 cut(s) 63, 79, 243
BstX2I RGATCY 2 cut(s) 138, 460
BstYI RGATCY 2 cut(s) 138, 460
BsuRI GGCC 1 cut(s) 177
BtsCI GGATG 2 cut(s) 268, 328
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 3 cut(s) 105, 111, 240
BveI ACCTGC 1 cut(s) 236
CaiI CAGNNNCTG 1 cut(s) 242
CfoI GCGC 1 cut(s) 484
Cfr13I GGNCC 1 cut(s) 176
CseI GACGC 1 cut(s) 578
Csp6I GTAC 1 cut(s) 470
CviAII CATG 5 cut(s) 47, 88, 180, 305, 476
CviQI GTAC 1 cut(s) 470
DdeI CTNAG 1 cut(s) 281
DpnI GATC 6 cut(s) 140, 448, 462, 520, 657, 690
DpnII GATC 6 cut(s) 138, 446, 460, 518, 655, 688
Eco24I GRGCYC 1 cut(s) 651
EcoT22I ATGCAT 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 651
FaeI CATG 5 cut(s) 50, 91, 183, 308, 479
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FaqI GGGAC 1 cut(s) 24
FatI CATG 5 cut(s) 46, 87, 179, 304, 475
FauNDI CATATG 2 cut(s) 585, 643
FbaI TGATCA 1 cut(s) 688
FokI GGATG 2 cut(s) 275, 335
FriOI GRGCYC 1 cut(s) 651
GlaI GCGC 1 cut(s) 483
GsaI CCCAGC 1 cut(s) 321
HaeII RGCGCY 1 cut(s) 485
HaeIII GGCC 1 cut(s) 177
HgaI GACGC 1 cut(s) 578
HhaI GCGC 1 cut(s) 484
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 5 cut(s) 50, 91, 183, 308, 479
Hin6I GCGC 1 cut(s) 482
HinP1I GCGC 1 cut(s) 482
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 4 cut(s) 12, 95, 408, 425
HphI GGTGA 2 cut(s) 104, 572
Hpy166II GTNNAC 3 cut(s) 225, 549, 580
Hpy188I TCNGA 4 cut(s) 143, 430, 446, 605
Hpy188III TCNNGA 2 cut(s) 380, 632
Hpy8I GTNNAC 3 cut(s) 225, 549, 580
HpyAV CCTTC 2 cut(s) 129, 205
HpyCH4III ACNGT 2 cut(s) 115, 469
HpyCH4IV ACGT 3 cut(s) 331, 384, 441
HpyCH4V TGCA 6 cut(s) 46, 65, 170, 245, 343, 570
HpyF10VI GCNNNNNNNGC 1 cut(s) 623
HpyF3I CTNAG 1 cut(s) 281
HpySE526I ACGT 3 cut(s) 331, 384, 441
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 5 cut(s) 50, 91, 183, 308, 479
HspAI GCGC 1 cut(s) 482
Ksp22I TGATCA 1 cut(s) 688
Kzo9I GATC 6 cut(s) 138, 446, 460, 518, 655, 688
LmnI GCTCC 1 cut(s) 350
LpnPI CCDG 9 cut(s) 56, 75, 231, 249, 303, 405, 495, 537, 636
LweI GCATC 2 cut(s) 261, 539
MaeII ACGT 3 cut(s) 331, 384, 441
MaeIII GTNAC 3 cut(s) 238, 494, 505
MalI GATC 6 cut(s) 140, 448, 462, 520, 657, 690
MboI GATC 6 cut(s) 138, 446, 460, 518, 655, 688
MboII GAAGA 2 cut(s) 380, 470
MflI RGATCY 2 cut(s) 138, 460
MhlI GDGCHC 1 cut(s) 651
MluCI AATT 5 cut(s) 57, 326, 401, 571, 607
MlyI GAGTC 1 cut(s) 6
MmeI TCCRAC 2 cut(s) 291, 436
MnlI CCTC 5 cut(s) 137, 253, 307, 378, 607
Mph1103I ATGCAT 1 cut(s) 172
MseI TTAA 4 cut(s) 129, 135, 149, 192
MspCI CTTAAG 2 cut(s) 128, 134
MwoI GCNNNNNNNGC 1 cut(s) 623
NdeI CATATG 2 cut(s) 585, 643
NdeII GATC 6 cut(s) 138, 446, 460, 518, 655, 688
NlaIII CATG 5 cut(s) 50, 91, 183, 308, 479
NmuCI GTSAC 1 cut(s) 238
NsiI ATGCAT 1 cut(s) 172
PaqCI CACCTGC 1 cut(s) 236
PfeI GAWTC 3 cut(s) 95, 408, 425
PflMI CCANNNNNTGG 1 cut(s) 310
PleI GAGTC 1 cut(s) 6
PpsI GAGTC 1 cut(s) 6
Ppu21I YACGTR 1 cut(s) 332
PspFI CCCAGC 1 cut(s) 317
PspPI GGNCC 1 cut(s) 176
PstI CTGCAG 2 cut(s) 67, 247
PstNI CAGNNNCTG 1 cut(s) 242
PsuI RGATCY 2 cut(s) 138, 460
RsaI GTAC 1 cut(s) 471
RsaNI GTAC 1 cut(s) 470
SaqAI TTAA 4 cut(s) 129, 135, 149, 192
Sau3AI GATC 6 cut(s) 138, 446, 460, 518, 655, 688
Sau96I GGNCC 1 cut(s) 176
SchI GAGTC 1 cut(s) 6
SduI GDGCHC 1 cut(s) 651
SfaNI GCATC 2 cut(s) 261, 539
SfcI CTRYAG 3 cut(s) 63, 79, 243
SmlI CTYRAG 2 cut(s) 128, 134
SmoI CTYRAG 2 cut(s) 128, 134
Sse9I AATT 5 cut(s) 57, 326, 401, 571, 607
SspI AATATT 2 cut(s) 30, 502
TaaI ACNGT 2 cut(s) 115, 469
TaiI ACGT 3 cut(s) 334, 387, 444
TasI AATT 5 cut(s) 57, 326, 401, 571, 607
TfiI GAWTC 3 cut(s) 95, 408, 425
Tru1I TTAA 4 cut(s) 129, 135, 149, 192
Tru9I TTAA 4 cut(s) 129, 135, 149, 192
TscAI CASTG 3 cut(s) 105, 118, 247
TseFI GTSAC 1 cut(s) 238
Tsp45I GTSAC 1 cut(s) 238
TspDTI ATGAA 7 cut(s) 17, 33, 339, 438, 492, 587, 630
TspRI CASTG 3 cut(s) 105, 118, 247
Van91I CCANNNNNTGG 1 cut(s) 310
Vha464I CTTAAG 2 cut(s) 128, 134
XapI RAATTY 1 cut(s) 401
ZraI GACGTC 1 cut(s) 442
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.