RchiOBHm_Chr4g0405381

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000654
Physical Location & Seq
Forward (+)
25865460 .. 25871733
6274 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37687

Sequence Viewer

Length: 3591 bp
ATGAGCACACAGTTGGATGCTTCTTCTTCTTCCTCGTCCTCCCCTTACCGGTGTACTTATCATGCATTCTTGAGTTTCAGAGGTGTGGACACACGTAAGGGATTTACCGATCATCTTTACAGGGCCTTGGAGCTCGCAGGAATCCACACGTTTAGAGACGATGATGAAATCGAGAGAGGAGCAAATATCGCAGCAGAGTTACAGAGAGCTATCGAAGAGTCGAGGGTATCTGTGGTTGTGTTGTCTAGAGACTATGCTGCATCAAGGTGGTGCTTGGACGAACTTGCAAAGATCATGGAGCGTAGGAGAAATGAAGACGCACACGTGGTTATTCCAGTGTTTTATGATGTAGATCCTTCCCATGTCAGGAAGCAAATCGGGAGTTTTGCTGAAGCTTTTGTTAGACATGAAGAGCGCTTCAAGGAGGAGATGGGAAAGGTTGCGGAGTGGAGAAGAGCGCTTTCTGATATTGCAGATCTTGGTGGAATGGTTTTAGGACAACGGTATGAATCACAATTTATCCAAGATATTGTTGAAGAAATCAGAAGTAAATTGGATTCCAAAGTCTTAAACATTGCTCCGTATGCAATTGGAATAGATGATCGTGTGCGAGGTATAAACATGTGGTTAGAAGATGGGTCAAATGATGTTGGTGTGGCTGTGATCTATGGAATGGGTGGAATTGGCAAAACCACTATTGCCAAAGCTGCTTATAACTTGAATTCCCATAGATTTCAAGGTAGCAGCTTTCTTGCTGATGTTAGAGAAACTTCAGAACAGCTGAATGGTTTGGTTCGCTTGCAAAGAAATCTTCTTTCAGATCTCCAAAAGGGGAAAACAAAGAAAATATACAACATTGATGAAGGAATACGAAAGATAAAAGATGCTGTATGTTGCAAGAAAGTTCTTATTGTTCTTGATGATGTGAACCACTCAGACCAATTTAATGCAATTATTGGAATGCGCAGATGGTTTGTTCCAGGAAGTAAGATAATCTTAACAACCAGATATGAACATTTGTTAAAGGCTCACGAAGTTAACACAATGTTTAAGGTTCAAGAGTTGAATGAGCATGAATCACTTGAGCTCCTCAGTTGGCACGCCTTTGGACAAGCCCATCCTATTGAAGCTTACATTGATCTTTCAAAACCCGTTGTAAAACATTGTGGAGGGATTCCATTAGCTCTGCAAGTTCTGGGATCTTCTTTGTCTGGCAGAAGTATAGATATATGGCAAAGCGCATTACAGAAGCTATGTGAGATTCCAGATGGAGAAATTCAAAAAATTCTTAGAATCAGTTTTGACTCTCTGCAAGATGATCATGACAAAAACTTATTCCTTCATATAGCATGCTTCTTCAGAGGGAAGAATAAAGAGTTTTCAATTACAGTACTGGACAACCTTAAATTCTACACAGTGATTGGAATTCAAAATCTGGTTGATAGATGTCTAGTGAAAATTAATGAAGACAACAAGTTGATAGTGCATCATTTGCTTCGAGACATGGCAAGGGGAATTATTCGCGAAGAATCACCTGAGGACCCTGGGAAACGCAGTAGGGTGTGGCACAAAGATGCCTTCAATATTTTGAGTAAAAGAACTGGTACTGGAACTGTTAAGGGCATCATGCTTGACCTTTCTATGTTGATGCAAGATGAGTCTTCCAAAACATTATTTAGTGGAAGTAACAGCAAACGACGTCGTGTTGATGATTATGATGGAAATTGTTCAGGACGGCGTCGACTTGGTCTCTTCACCTGGCAATTATTTAGTTTTTCCTCAGCCAACACGACTCCAGCGTCAGATGAGGTTGACCTCAAAATAGAGGCATTCAAAAAGATGCACAACCTTGAAGTTCTCCTACTCAATAATGTACATTTCAGTCAAGGCTTTGAAAATTTTCCAAAAAACTTGATATGGCTGTCTTGGCGAGGATTCTCTTTAAATTCGATACCAGCCAGTTTGTATTTGAAAACTCTAGTTGTTCTTGACTTGCGGAATAGCAGCCTGCAATGTGTTTGGAAGGGAACCAAGTCTCTTCCAAGTCTGAAAGTCCTTAATCTTAGTCATTCACATGGCCTTTCAGCAACCCCCGACATATCAGGACTCCCTAACCTGGAGAGATTGATTCTAAAAAATTGCAGAAATTTGATTGAGGTTGATGAATCGATTGGAGACTTGAAGAAACTTGTTTTCTTAAATCTAAAAGACTGCAAAAATCTGATGAAGCTTCCGACCAGAATCAAAATGTCAAGTTCTCTTCAAACACTTATTCTTTCTGGTTGCTCAAAGCTTGTGCTTCATTCTAATATGGTTGCAACAAATCAGTCACTCTTGACAGCTAGTGATATGAAGAAAGACGATTGTTCATCACCTAACTTGTGGAAATCACTCTTATTATGGGCAGTACCAAGCAGAAATATCATACCAACCAGTTTCTCAGTATCAAATTTACCACGTTCTTTACGAAGCTTAAGCTTAGCTGACTGCAACCTATTAGAGATTCCAGGTGATCTTAGTATCCTCTCCTCATTGAAGTATTTAGACCTATGTGGGAACCCAATTCAGAGCCTCCCAGAAAACATAAGATCTCTTAGTAAGCTTGAGACACTTATGTTAGAGGACTGCACAGAACTCAGAACTCTTCCAGAGCTCCCACCAAGTCTCCAGAGTTTGCTTGCAAGTTCTTGTAAATCATTGAAAAGAATAACAAATTTACCAAACTTGTTCAAATCATTGGATACTGATTTTCTGCATTGCAAGAAGTTGGTTGAAGTTGAAAGCTTGTTCACTATAAAACCATTGACAACCACTGACATAGAAATGATCAAAGATATGGGACTGTTCAATTTTAAATCCATTGGAAGCGACGCTGAGGTTGAAATGATCAACTACATTACAAATACAACCAAGAAGGTCCCCCTCCAGGTGTTGTCTGAATGTGGCATATCTAGCATTTACCTCAACGGAAACGAGATTCCAGATTGGTTTCGCCACAGGAGAATGGGCAACTCTGTGTTGTCTATTATAGTACCTCCACATCTTAATCATAGAATTCGAGGCTTAAATGCATGTGTTGTATATGCACGACGCCCAGACATTCGTCGTTCTGCTAAATATCAAAACTTGTTTAAAGTCAGCAATGAGACCAAGGGTCTCATGTGGACCTATTCCCCTATCACAATAGGTATTCCAAAGGAGAATGAAGACATGTTATGGCTAAGCCATTGGCGGTTTGGAGAACATGATCTGGGAGGGGGAGACGAAGTACGTGTTTCGGTTGAGTTTGGACCCGAGCTTGGACACGATCTAAATTTATGGACAAAGGAATTGGGAATCCAACTTGTGTATGAGCAGGAAAGTAGTGAAGATGGAAAGAAACACAATTTCCTTGCTGGAGATGTGTCAGTGTCATCCTCAAGTTACCAAATGTGGACGGGAAAATACTTTCTTTGTAATAACCTGAATCGTGCTCAGAAAACACAATTCAAAAGTAGCCAGGAAAACCCATCTCATCTTGATTCTGACAAAACCAAGAGCCCCTTCCTTTTCTTATTTGAGCATGATGATGTTTCACAGGCTGATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1196

Amino Acids

135.35

Weight (kDa)

7.76

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 188 6.9e-54 TIR domain
TIR_2 PF13676 23 - 120 7.3e-18 TIR domain
NB-ARC PF00931 201 - 369 1.6e-20 NB-ARC domain
WHD_ROQ1 PF23282 440 - 511 5.8e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 650 - 761 5.1e-06 Leucine-rich repeat region
LRR_14 PF23598 820 - 913 1.8e-08 Leucine-rich repeat region
LRR_8 PF13855 820 - 876 2.5e-06 Leucine rich repeat
C-JID PF20160 989 - 1121 2.5e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
AasI GACNNNNNNGTC 1 cut(s) 1798
AatII GACGTC 1 cut(s) 1702
Acc16I TGCGCA 1 cut(s) 965
AccI GTMKAC 1 cut(s) 1741
AccII CGCG 1 cut(s) 1524
AciI CCGC 3 cut(s) 443, 1996, 3232
AclWI GGATC 2 cut(s) 347, 1207
AcuI CTGAAG 3 cut(s) 411, 756, 1342
AcvI CACGTG 1 cut(s) 325
AcyI GRCGYC 3 cut(s) 1699, 1738, 3091
AfaI GTAC 7 cut(s) 55, 1392, 1606, 1875, 2409, 3033, 3270
AfeI AGCGCT 2 cut(s) 416, 459
AfiI CCNNNNNNNGG 5 cut(s) 48, 366, 2116, 2926, 3299
AflII CTTAAG 1 cut(s) 2473
AflIII ACRYGT 6 cut(s) 92, 147, 322, 621, 3210, 3271
AgeI ACCGGT 1 cut(s) 48
AhdI GACNNNNNGTC 1 cut(s) 3153
AjnI CCWGG 7 cut(s) 979, 1543, 1757, 2115, 2506, 2925, 3498
AjuI GAANNNNNNNTTGG 2 cut(s) 3527, 3559
Alw21I GWGCWC 5 cut(s) 8, 135, 1089, 2655, 3475
AlwI GGATC 2 cut(s) 347, 1207
Ama87I CYCGRG 1 cut(s) 3293
Aor51HI AGCGCT 2 cut(s) 416, 459
AoxI GGCC 2 cut(s) 123, 2077
ApeKI GCWGC 5 cut(s) 191, 257, 707, 744, 2004
AseI ATTAAT 1 cut(s) 1461
AsiGI ACCGGT 1 cut(s) 48
Asp700I GAANNNNTTC 2 cut(s) 1726, 1899
AspLEI GCGC 4 cut(s) 417, 460, 966, 1241
AspS9I GGNCC 5 cut(s) 123, 1540, 2917, 3165, 3290
AsuHPI GGTGA 4 cut(s) 1524, 1747, 2364, 2522
AvaI CYCGRG 1 cut(s) 3293
AvaII GGWCC 4 cut(s) 1540, 2917, 3165, 3290
AxyI CCTNAGG 1 cut(s) 1536
BanII GRGCYC 4 cut(s) 135, 1089, 2655, 3542
BarI GAAGNNNNNNTAC 2 cut(s) 1845, 1877
BbrPI CACGTG 1 cut(s) 325
BbsI GAAGAC 4 cut(s) 321, 1473, 1653, 3213
Bbv12I GWGCWC 5 cut(s) 8, 135, 1089, 2655, 3475
BbvCI CCTCAGC 2 cut(s) 1780, 2874
BbvI GCAGC 5 cut(s) 203, 244, 694, 756, 2016
BccI CCATC 8 cut(s) 424, 629, 963, 1125, 1262, 1712, 3365, 3517
BceAI ACGGC 1 cut(s) 1751
BciT130I CCWGG 7 cut(s) 981, 1545, 1759, 2117, 2508, 2927, 3500
BciVI GTATCC 2 cut(s) 2531, 2734
BclI TGATCA 4 cut(s) 1318, 2826, 2886, 3583
BfaI CTAG 5 cut(s) 246, 1451, 1979, 2343, 2952
BfoI RGCGCY 2 cut(s) 418, 461
BfrI CTTAAG 1 cut(s) 2473
BfuI GTATCC 2 cut(s) 2531, 2734
BglII AGATCT 3 cut(s) 475, 820, 2588
BisI GCNGC 5 cut(s) 192, 258, 708, 745, 2005
BlpI GCTNAGC 2 cut(s) 2479, 3221
BlsI GCNGC 5 cut(s) 193, 259, 709, 746, 2006
BmcAI AGTACT 1 cut(s) 1392
Bme1390I CCNGG 7 cut(s) 981, 1545, 1759, 2117, 2508, 2927, 3500
Bme18I GGWCC 4 cut(s) 1540, 2917, 3165, 3290
BmeRI GACNNNNNGTC 1 cut(s) 3153
BmeT110I CYCGRG 1 cut(s) 3293
BmgT120I GGNCC 5 cut(s) 123, 1540, 2917, 3165, 3290
BmiI GGNNCC 5 cut(s) 1542, 2029, 2558, 2919, 3292
BmrFI CCNGG 7 cut(s) 981, 1545, 1759, 2117, 2508, 2927, 3500
BmsI GCATC 8 cut(s) 7, 269, 874, 1495, 1564, 1632, 1638, 1830
BoxI GACNNNNGTC 1 cut(s) 3102
BpiI GAAGAC 4 cut(s) 321, 1473, 1653, 3213
BpmI CTGGAG 5 cut(s) 1779, 2138, 2651, 2909, 3417
Bpu10I CCTNAGC 2 cut(s) 1780, 2874
Bpu1102I GCTNAGC 2 cut(s) 2479, 3221
BpuEI CTTGAG 4 cut(s) 91, 1103, 2624, 3403
Bsa29I ATCGAT 1 cut(s) 2168
BsaAI YACGTR 3 cut(s) 95, 325, 3272
BsaBI GATNNNNATC 1 cut(s) 351
BsaHI GRCGYC 3 cut(s) 1699, 1738, 3091
BsaI GGTCTC 3 cut(s) 1754, 3140, 3161
BsaJI CCNNGG 4 cut(s) 126, 1543, 1544, 3150
BsaWI WCCGGW 1 cut(s) 48
BsaXI ACNNNNNCTCC 6 cut(s) 1071, 1101, 2649, 2679, 3252, 3282
Bsc4I CCNNNNNNNGG 5 cut(s) 48, 366, 2116, 2926, 3299
Bse118I RCCGGY 1 cut(s) 48
Bse1I ACTGG 6 cut(s) 335, 1398, 1606, 1612, 1959, 2433
Bse21I CCTNAGG 1 cut(s) 1536
Bse3DI GCAATG 4 cut(s) 573, 2018, 2755, 3148
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 7 cut(s) 981, 1545, 1759, 2117, 2508, 2927, 3500
BseCI ATCGAT 1 cut(s) 2168
BseDI CCNNGG 4 cut(s) 126, 1543, 1544, 3150
BseGI GGATG 3 cut(s) 22, 1117, 3413
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 5 cut(s) 48, 366, 2116, 2926, 3299
BseMI GCAATG 4 cut(s) 573, 2018, 2755, 3148
BseMII CTCAG 8 cut(s) 948, 1105, 1527, 1794, 2454, 2650, 2865, 3488
BseNI ACTGG 6 cut(s) 335, 1398, 1606, 1612, 1959, 2433
BseRI GAGGAG 4 cut(s) 192, 440, 1079, 2518
BseXI GCAGC 5 cut(s) 203, 244, 694, 756, 2016
BsgI GTGCAG 1 cut(s) 2611
Bsh1236I CGCG 1 cut(s) 1524
BshFI GGCC 2 cut(s) 125, 2079
BshTI ACCGGT 1 cut(s) 48
BshVI ATCGAT 1 cut(s) 2168
BsiHKAI GWGCWC 5 cut(s) 8, 135, 1089, 2655, 3475
BsiHKCI CYCGRG 1 cut(s) 3293
BsiSI CCGG 1 cut(s) 49
BslFI GGGAC 2 cut(s) 2853, 2903
BslI CCNNNNNNNGG 5 cut(s) 48, 366, 2116, 2926, 3299
BsmBI CGTCTC 2 cut(s) 150, 3255
BsmFI GGGAC 2 cut(s) 2853, 2903
BsmI GAATGC 3 cut(s) 65, 966, 1829
BsnI GGCC 2 cut(s) 125, 2079
Bso31I GGTCTC 3 cut(s) 1754, 3140, 3161
BsoBI CYCGRG 1 cut(s) 3293
Bsp1286I GDGCHC 6 cut(s) 8, 135, 1089, 2655, 3475, 3542
Bsp1407I TGTACA 1 cut(s) 1873
Bsp1720I GCTNAGC 2 cut(s) 2479, 3221
Bsp68I TCGCGA 1 cut(s) 1524
BspACI CCGC 3 cut(s) 443, 1996, 3232
BspANI GGCC 2 cut(s) 125, 2079
BspCNI CTCAG 8 cut(s) 947, 1104, 1528, 1793, 2453, 2649, 2866, 3487
BspDI ATCGAT 1 cut(s) 2168
BspFNI CGCG 1 cut(s) 1524
BspHI TCATGA 1 cut(s) 1321
BspLI GGNNCC 5 cut(s) 1542, 2029, 2558, 2919, 3292
BspPI GGATC 2 cut(s) 347, 1207
BspQI GCTCTTC 2 cut(s) 405, 448
BspTI CTTAAG 1 cut(s) 2473
BspTNI GGTCTC 3 cut(s) 1754, 3140, 3161
BsrDI GCAATG 4 cut(s) 573, 2018, 2755, 3148
BsrFI RCCGGY 1 cut(s) 48
BsrGI TGTACA 1 cut(s) 1873
BsrI ACTGG 6 cut(s) 335, 1398, 1606, 1612, 1959, 2433
BssAI RCCGGY 1 cut(s) 48
BssECI CCNNGG 4 cut(s) 126, 1543, 1544, 3150
BssNI GRCGYC 3 cut(s) 1699, 1738, 3091
BssT1I CCWWGG 2 cut(s) 126, 3150
Bst2UI CCWGG 7 cut(s) 981, 1545, 1759, 2117, 2508, 2927, 3500
Bst4CI ACNGT 6 cut(s) 12, 504, 1390, 1417, 1615, 2844
Bst6I CTCTTC 7 cut(s) 210, 405, 448, 1757, 2043, 2265, 2649
BstACI GRCGYC 3 cut(s) 1699, 1738, 3091
BstAFI CTTAAG 1 cut(s) 2473
BstAPI GCANNNNNTGC 1 cut(s) 1492
BstAUI TGTACA 1 cut(s) 1873
BstBAI YACGTR 3 cut(s) 95, 325, 3272
BstC8I GCNNGC 6 cut(s) 135, 800, 1101, 1351, 2009, 2679
BstF5I GGATG 3 cut(s) 22, 1117, 3413
BstFNI CGCG 1 cut(s) 1524
BstH2I RGCGCY 2 cut(s) 418, 461
BstHHI GCGC 4 cut(s) 417, 460, 966, 1241
BstMWI GCNNNNNNNGC 6 cut(s) 188, 584, 707, 1492, 1927, 2952
BstNI CCWGG 7 cut(s) 981, 1545, 1759, 2117, 2508, 2927, 3500
BstNSI RCATGY 4 cut(s) 625, 1353, 3075, 3214
BstPAI GACNNNNGTC 1 cut(s) 3102
BstSCI CCNGG 7 cut(s) 979, 1543, 1757, 2115, 2506, 2925, 3498
BstUI CGCG 1 cut(s) 1524
BstV1I GCAGC 5 cut(s) 203, 244, 694, 756, 2016
BstV2I GAAGAC 4 cut(s) 321, 1473, 1653, 3213
BstX2I RGATCY 5 cut(s) 352, 475, 820, 1199, 2588
BstYI RGATCY 5 cut(s) 352, 475, 820, 1199, 2588
Bsu15I ATCGAT 1 cut(s) 2168
Bsu36I CCTNAGG 1 cut(s) 1536
BsuI GTATCC 2 cut(s) 2531, 2734
BsuRI GGCC 2 cut(s) 125, 2079
BsuTUI ATCGAT 1 cut(s) 2168
BtsCI GGATG 3 cut(s) 22, 1117, 3413
BtsIMutI CAGTG 4 cut(s) 342, 1422, 2811, 3414
BtuMI TCGCGA 1 cut(s) 1524
Cac8I GCNNGC 6 cut(s) 135, 800, 1101, 1351, 2009, 2679
CciI TCATGA 1 cut(s) 1321
CfoI GCGC 4 cut(s) 417, 460, 966, 1241
Cfr10I RCCGGY 1 cut(s) 48
Cfr13I GGNCC 5 cut(s) 123, 1540, 2917, 3165, 3290
ClaI ATCGAT 1 cut(s) 2168
CseI GACGC 5 cut(s) 326, 1727, 1788, 2879, 3099
Csp6I GTAC 7 cut(s) 54, 1391, 1605, 1874, 2408, 3032, 3269
CspAI ACCGGT 1 cut(s) 48
CviQI GTAC 7 cut(s) 54, 1391, 1605, 1874, 2408, 3032, 3269
DraI TTTAAA 3 cut(s) 1944, 2854, 3133
DrdI GACNNNNNNGTC 1 cut(s) 1798
DriI GACNNNNNGTC 1 cut(s) 3153
DseDI GACNNNNNNGTC 1 cut(s) 1798
Eam1104I CTCTTC 7 cut(s) 210, 405, 448, 1757, 2043, 2265, 2649
Eam1105I GACNNNNNGTC 1 cut(s) 3153
EarI CTCTTC 7 cut(s) 210, 405, 448, 1757, 2043, 2265, 2649
Ecl136II GAGCTC 3 cut(s) 133, 1087, 2653
Eco130I CCWWGG 2 cut(s) 126, 3150
Eco24I GRGCYC 4 cut(s) 135, 1089, 2655, 3542
Eco31I GGTCTC 3 cut(s) 1754, 3140, 3161
Eco47I GGWCC 4 cut(s) 1540, 2917, 3165, 3290
Eco47III AGCGCT 2 cut(s) 416, 459
Eco53kI GAGCTC 3 cut(s) 133, 1087, 2653
Eco57I CTGAAG 3 cut(s) 411, 756, 1342
Eco72I CACGTG 1 cut(s) 325
Eco81I CCTNAGG 1 cut(s) 1536
Eco88I CYCGRG 1 cut(s) 3293
EcoICRI GAGCTC 3 cut(s) 133, 1087, 2653
EcoO109I RGGNCCY 3 cut(s) 123, 1540, 2917
EcoRI GAATTC 3 cut(s) 721, 1425, 3054
EcoRII CCWGG 7 cut(s) 979, 1543, 1757, 2115, 2506, 2925, 3498
EcoT14I CCWWGG 2 cut(s) 126, 3150
EcoT22I ATGCAT 2 cut(s) 67, 3073
EcoT38I GRGCYC 4 cut(s) 135, 1089, 2655, 3542
ErhI CCWWGG 2 cut(s) 126, 3150
Esp3I CGTCTC 2 cut(s) 150, 3255
FalI AAGNNNNNCTT 4 cut(s) 980, 1012, 3527, 3559
FaqI GGGAC 2 cut(s) 2853, 2903
FbaI TGATCA 4 cut(s) 1318, 2826, 2886, 3583
FblI GTMKAC 1 cut(s) 1741
Fnu4HI GCNGC 5 cut(s) 192, 258, 708, 745, 2005
FokI GGATG 3 cut(s) 29, 1104, 3400
FriOI GRGCYC 4 cut(s) 135, 1089, 2655, 3542
Fsp4HI GCNGC 5 cut(s) 192, 258, 708, 745, 2005
FspBI CTAG 5 cut(s) 246, 1451, 1979, 2343, 2952
FspI TGCGCA 1 cut(s) 965
GlaI GCGC 4 cut(s) 416, 459, 965, 1240
GluI GCNGC 5 cut(s) 192, 258, 708, 745, 2005
GsuI CTGGAG 5 cut(s) 1779, 2138, 2651, 2909, 3417
HaeII RGCGCY 2 cut(s) 418, 461
HaeIII GGCC 2 cut(s) 125, 2079
HapII CCGG 1 cut(s) 49
HgaI GACGC 5 cut(s) 326, 1727, 1788, 2879, 3099
HhaI GCGC 4 cut(s) 417, 460, 966, 1241
Hin1I GRCGYC 3 cut(s) 1699, 1738, 3091
Hin6I GCGC 4 cut(s) 415, 458, 964, 1239
HinP1I GCGC 4 cut(s) 415, 458, 964, 1239
HincII GTYRAC 3 cut(s) 1039, 1742, 1813
HindII GTYRAC 3 cut(s) 1039, 1742, 1813
HindIII AAGCTT 8 cut(s) 393, 1128, 2228, 2291, 2470, 2476, 2600, 2782
HpaI GTTAAC 1 cut(s) 1039
HpaII CCGG 1 cut(s) 49
HphI GGTGA 4 cut(s) 1524, 1747, 2364, 2522
Hpy166II GTNNAC 9 cut(s) 54, 88, 928, 1039, 1742, 1813, 2790, 3165, 3435
Hpy8I GTNNAC 9 cut(s) 54, 88, 928, 1039, 1742, 1813, 2790, 3165, 3435
Hpy99I CGWCG 6 cut(s) 1701, 1704, 1743, 2873, 3093, 3108
HpyAV CCTTC 7 cut(s) 366, 857, 1349, 1588, 2017, 2908, 3553
HpyCH4III ACNGT 6 cut(s) 12, 504, 1390, 1417, 1615, 2844
HpyCH4IV ACGT 6 cut(s) 94, 149, 324, 1699, 2458, 3271
HpyF10VI GCNNNNNNNGC 6 cut(s) 188, 584, 707, 1492, 1927, 2952
HpySE526I ACGT 6 cut(s) 94, 149, 324, 1699, 2458, 3271
Hsp92I GRCGYC 3 cut(s) 1699, 1738, 3091
HspAI GCGC 4 cut(s) 415, 458, 964, 1239
Ksp22I TGATCA 4 cut(s) 1318, 2826, 2886, 3583
KspAI GTTAAC 1 cut(s) 1039
LguI GCTCTTC 2 cut(s) 405, 448
LmnI GCTCC 6 cut(s) 130, 179, 298, 583, 1092, 2658
Lsp1109I GCAGC 5 cut(s) 203, 244, 694, 756, 2016
LweI GCATC 8 cut(s) 7, 269, 874, 1495, 1564, 1632, 1638, 1830
MaeI CTAG 5 cut(s) 246, 1451, 1979, 2343, 2952
MaeII ACGT 6 cut(s) 94, 149, 324, 1699, 2458, 3271
MaeIII GTNAC 4 cut(s) 198, 1685, 2328, 3422
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 5 cut(s) 352, 475, 820, 1199, 2588
MhlI GDGCHC 6 cut(s) 8, 135, 1089, 2655, 3475, 3542
MlyI GAGTC 5 cut(s) 227, 1298, 1667, 1786, 2100
MmeI TCCRAC 2 cut(s) 2258, 3364
Mph1103I ATGCAT 2 cut(s) 67, 3073
MroXI GAANNNNTTC 2 cut(s) 1726, 1899
MslI CAYNNNNRTG 5 cut(s) 265, 1572, 2073, 2822, 3567
MspA1I CMGCKG 1 cut(s) 781
MspCI CTTAAG 1 cut(s) 2473
MspI CCGG 1 cut(s) 49
MspR9I CCNGG 7 cut(s) 981, 1545, 1759, 2117, 2508, 2927, 3500
MunI CAATTG 1 cut(s) 588
Mva1269I GAATGC 3 cut(s) 65, 966, 1829
MvaI CCWGG 7 cut(s) 981, 1545, 1759, 2117, 2508, 2927, 3500
MvnI CGCG 1 cut(s) 1524
MwoI GCNNNNNNNGC 6 cut(s) 188, 584, 707, 1492, 1927, 2952
NlaIV GGNNCC 5 cut(s) 1542, 2029, 2558, 2919, 3292
NmuCI GTSAC 1 cut(s) 2328
NruI TCGCGA 1 cut(s) 1524
NsbI TGCGCA 1 cut(s) 965
NsiI ATGCAT 2 cut(s) 67, 3073
NspI RCATGY 4 cut(s) 625, 1353, 3075, 3214
PaeI GCATGC 1 cut(s) 1353
PagI TCATGA 1 cut(s) 1321
PasI CCCWGGG 1 cut(s) 1544
PciI ACATGT 2 cut(s) 621, 3210
PciSI GCTCTTC 2 cut(s) 405, 448
PcsI WCGNNNNNNNCGW 2 cut(s) 1796, 2464
PctI GAATGC 3 cut(s) 65, 966, 1829
PdmI GAANNNNTTC 2 cut(s) 1726, 1899
PflFI GACNNNGTC 2 cut(s) 1737, 1746
PfoI TCCNGGA 1 cut(s) 979
PinAI ACCGGT 1 cut(s) 48
PkrI GCNGC 5 cut(s) 193, 259, 709, 746, 2006
PleI GAGTC 5 cut(s) 226, 1298, 1666, 1786, 2100
PmaCI CACGTG 1 cut(s) 325
PmlI CACGTG 1 cut(s) 325
PpsI GAGTC 5 cut(s) 226, 1298, 1666, 1786, 2100
Ppu21I YACGTR 3 cut(s) 95, 325, 3272
PpuMI RGGWCCY 2 cut(s) 1540, 2917
PscI ACATGT 2 cut(s) 621, 3210
PshAI GACNNNNGTC 1 cut(s) 3102
PshBI ATTAAT 1 cut(s) 1461
PsiI TTATAA 1 cut(s) 714
Psp124BI GAGCTC 3 cut(s) 135, 1089, 2655
Psp5II RGGWCCY 2 cut(s) 1540, 2917
Psp6I CCWGG 7 cut(s) 979, 1543, 1757, 2115, 2506, 2925, 3498
PspCI CACGTG 1 cut(s) 325
PspGI CCWGG 7 cut(s) 979, 1543, 1757, 2115, 2506, 2925, 3498
PspN4I GGNNCC 5 cut(s) 1542, 2029, 2558, 2919, 3292
PspPI GGNCC 5 cut(s) 123, 1540, 2917, 3165, 3290
PspPPI RGGWCCY 2 cut(s) 1540, 2917
PsuI RGATCY 5 cut(s) 352, 475, 820, 1199, 2588
PsyI GACNNNGTC 2 cut(s) 1737, 1746
PvuII CAGCTG 1 cut(s) 781
RruI TCGCGA 1 cut(s) 1524
RsaI GTAC 7 cut(s) 55, 1392, 1606, 1875, 2409, 3033, 3270
RsaNI GTAC 7 cut(s) 54, 1391, 1605, 1874, 2408, 3032, 3269
RseI CAYNNNNRTG 5 cut(s) 265, 1572, 2073, 2822, 3567
SacI GAGCTC 3 cut(s) 135, 1089, 2655
SalI GTCGAC 1 cut(s) 1740
SapI GCTCTTC 2 cut(s) 405, 448
SatI GCNGC 5 cut(s) 192, 258, 708, 745, 2005
Sau96I GGNCC 5 cut(s) 123, 1540, 2917, 3165, 3290
ScaI AGTACT 1 cut(s) 1392
SchI GAGTC 5 cut(s) 227, 1298, 1667, 1786, 2100
ScrFI CCNGG 7 cut(s) 981, 1545, 1759, 2117, 2508, 2927, 3500
SduI GDGCHC 6 cut(s) 8, 135, 1089, 2655, 3475, 3542
SfaNI GCATC 8 cut(s) 7, 269, 874, 1495, 1564, 1632, 1638, 1830
SinI GGWCC 4 cut(s) 1540, 2917, 3165, 3290
SmiMI CAYNNNNRTG 5 cut(s) 265, 1572, 2073, 2822, 3567
SmlI CTYRAG 5 cut(s) 70, 1082, 2473, 2603, 3418
SmoI CTYRAG 5 cut(s) 70, 1082, 2473, 2603, 3418
SphI GCATGC 1 cut(s) 1353
SsiI CCGC 3 cut(s) 443, 1996, 3232
SspI AATATT 1 cut(s) 1585
SspMI CTAG 5 cut(s) 246, 1451, 1979, 2343, 2952
SstI GAGCTC 3 cut(s) 135, 1089, 2655
StyD4I CCNGG 7 cut(s) 979, 1543, 1757, 2115, 2506, 2925, 3498
StyI CCWWGG 2 cut(s) 126, 3150
TaaI ACNGT 6 cut(s) 12, 504, 1390, 1417, 1615, 2844
TaiI ACGT 6 cut(s) 97, 152, 327, 1702, 2461, 3274
TaqI TCGA 8 cut(s) 171, 213, 221, 1498, 1741, 1949, 2168, 3058
TatI WGTACW 3 cut(s) 53, 1390, 1873
TscAI CASTG 4 cut(s) 342, 1422, 2818, 3414
TseFI GTSAC 1 cut(s) 2328
TseI GCWGC 5 cut(s) 191, 257, 707, 744, 2004
Tsp45I GTSAC 1 cut(s) 2328
TspGWI ACGGA 2 cut(s) 570, 2982
TspRI CASTG 4 cut(s) 342, 1422, 2818, 3414
Tth111I GACNNNGTC 2 cut(s) 1737, 1746
Vha464I CTTAAG 1 cut(s) 2473
VpaK11BI GGWCC 4 cut(s) 1540, 2917, 3165, 3290
VspI ATTAAT 1 cut(s) 1461
XbaI TCTAGA 1 cut(s) 245
XceI RCATGY 4 cut(s) 625, 1353, 3075, 3214
XcmI CCANNNNNNNNNTGG 1 cut(s) 3233
XmiI GTMKAC 1 cut(s) 1741
XmnI GAANNNNTTC 2 cut(s) 1726, 1899
XspI CTAG 5 cut(s) 246, 1451, 1979, 2343, 2952
ZraI GACGTC 1 cut(s) 1700
ZrmI AGTACT 1 cut(s) 1392
Zsp2I ATGCAT 2 cut(s) 67, 3073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.