MD15G1376100.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000654
Physical Location & Seq
Forward (+)
45994453 .. 45995393
941 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1376100.v1.1.491

Sequence Viewer

Length: 735 bp
ATGAAGCTTCCAAAAAGAATGAAAATGTTGAGGTCTCTTGAGGAACTTATTCTTACTGGTTGCTCAAAGCTTGGTCCTCATGACAGTGCCACGGTTATTGATTTACTCGCTACATCTAGGGATATGAATAAACTTAGACTGTTATCAACTATATCATGGACACCTATCAGGTATTGGTGGATGTGGGCATGGCCGAGAAAGACTCTTCAATCAACCAGTTTCTCACTGGCAAGTTTGCCACGTTGTTTGGGAAGCTTAAGTCTGTCTTACTGCAACGTGTCAGAGGTTCCCAATGATCTGTGTACACTATCTTTGTTGAAGCATTTGAATCTAACTGGTAACCCAATTTTGTGCCTACCACAAAAAATGAAGAGTCTTATTATGCTCGAGACTCTTTTGTTAGATAATTGCACAAACCTCGAAATTCTTCCAGAGCTCCCACCAAGGTTGAAGAGGTTAGAAGCAAAGTATTGTACTTCATTGAAAAGATTAACAAATTTACCAAACTTGTTCAGATCATTGGAATCACTTTTTTGGGGTTGTGAGCATTTAGTTGAAGTTGAAAGCTTGTTGAATATAAAACCGGTGAGAAGCGCTGACATAGAAATGACAAGATATATGGGCCTGTTCAATTTGAAATCCATAGCAAGCACTGATGTCGAAATGATGAACTACTTGACCGGTACAAGCAGGAAGGCTCCTGTCCAGATTCTTGATGAAACAGGTACTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

245

Amino Acids

27.79

Weight (kDa)

9.02

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 191
AcsI RAATTY 2 cut(s) 423, 496
AfaI GTAC 4 cut(s) 304, 475, 685, 727
AfeI AGCGCT 1 cut(s) 595
AflII CTTAAG 1 cut(s) 256
AflIII ACRYGT 1 cut(s) 276
AgeI ACCGGT 2 cut(s) 583, 680
AluBI AGCT 5 cut(s) 7, 70, 255, 436, 567
AluI AGCT 5 cut(s) 7, 70, 255, 436, 567
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 2 cut(s) 39, 383
Ama87I CYCGRG 1 cut(s) 386
Aor51HI AGCGCT 1 cut(s) 595
AoxI GGCC 2 cut(s) 191, 622
ApoI RAATTY 2 cut(s) 423, 496
AsiGI ACCGGT 2 cut(s) 583, 680
Asp700I GAANNNNTTC 2 cut(s) 48, 426
AspLEI GCGC 1 cut(s) 596
AspS9I GGNCC 2 cut(s) 74, 622
AsuHPI GGTGA 1 cut(s) 598
AvaI CYCGRG 1 cut(s) 386
AvaII GGWCC 1 cut(s) 74
BanII GRGCYC 1 cut(s) 438
Bbv12I GWGCWC 1 cut(s) 438
BcgI CGANNNNNNTGC 4 cut(s) 400, 434, 640, 674
BcoDI GTCTC 2 cut(s) 39, 383
BfaI CTAG 1 cut(s) 117
BfoI RGCGCY 1 cut(s) 597
BfrI CTTAAG 1 cut(s) 256
Bme18I GGWCC 1 cut(s) 74
BmeT110I CYCGRG 1 cut(s) 386
BmgT120I GGNCC 2 cut(s) 74, 622
BmiI GGNNCC 2 cut(s) 288, 699
BplI GAGNNNNNCTC 2 cut(s) 187, 219
BpuEI CTTGAG 1 cut(s) 59
BsaI GGTCTC 1 cut(s) 39
BsaJI CCNNGG 2 cut(s) 90, 443
BsaWI WCCGGW 2 cut(s) 583, 680
Bse118I RCCGGY 2 cut(s) 583, 680
Bse1I ACTGG 4 cut(s) 61, 216, 231, 340
BseDI CCNNGG 2 cut(s) 90, 443
BseGI GGATG 1 cut(s) 186
BseNI ACTGG 4 cut(s) 61, 216, 231, 340
BshFI GGCC 2 cut(s) 193, 624
BshTI ACCGGT 2 cut(s) 583, 680
BsiHKAI GWGCWC 1 cut(s) 438
BsiHKCI CYCGRG 1 cut(s) 386
BsiSI CCGG 2 cut(s) 584, 681
BsmAI GTCTC 2 cut(s) 39, 383
BsnI GGCC 2 cut(s) 193, 624
Bso31I GGTCTC 1 cut(s) 39
BsoBI CYCGRG 1 cut(s) 386
Bsp1286I GDGCHC 1 cut(s) 438
Bsp1407I TGTACA 1 cut(s) 302
Bsp143I GATC 2 cut(s) 295, 515
BspANI GGCC 2 cut(s) 193, 624
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 2 cut(s) 288, 699
BspTI CTTAAG 1 cut(s) 256
BspTNI GGTCTC 1 cut(s) 39
BsrFI RCCGGY 2 cut(s) 583, 680
BsrGI TGTACA 1 cut(s) 302
BsrI ACTGG 4 cut(s) 61, 216, 231, 340
BssAI RCCGGY 2 cut(s) 583, 680
BssECI CCNNGG 2 cut(s) 90, 443
BssMI GATC 2 cut(s) 295, 515
BssT1I CCWWGG 1 cut(s) 443
Bst4CI ACNGT 3 cut(s) 86, 94, 141
Bst6I CTCTTC 3 cut(s) 210, 365, 446
BstAFI CTTAAG 1 cut(s) 256
BstAUI TGTACA 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 649
BstDEI CTNAG 1 cut(s) 134
BstDSI CCRYGG 1 cut(s) 90
BstEII GGTNACC 1 cut(s) 338
BstF5I GGATG 1 cut(s) 186
BstH2I RGCGCY 1 cut(s) 597
BstHHI GCGC 1 cut(s) 596
BstKTI GATC 2 cut(s) 298, 518
BstMAI GTCTC 2 cut(s) 39, 383
BstMBI GATC 2 cut(s) 295, 515
BstPI GGTNACC 1 cut(s) 338
BsuRI GGCC 2 cut(s) 193, 624
BtgI CCRYGG 1 cut(s) 90
BtsCI GGATG 1 cut(s) 186
BtsIMutI CAGTG 3 cut(s) 91, 224, 651
Cac8I GCNNGC 1 cut(s) 649
CciI TCATGA 1 cut(s) 79
CfoI GCGC 1 cut(s) 596
Cfr10I RCCGGY 2 cut(s) 583, 680
Cfr13I GGNCC 2 cut(s) 74, 622
Csp6I GTAC 4 cut(s) 303, 474, 684, 726
CspAI ACCGGT 2 cut(s) 583, 680
CspCI CAANNNNNGTGG 6 cut(s) 79, 114, 228, 263, 429, 464
CviAII CATG 3 cut(s) 80, 156, 189
CviJI RGCY 8 cut(s) 7, 70, 193, 255, 436, 567, 624, 698
CviKI_1 RGCY 8 cut(s) 7, 70, 193, 255, 436, 567, 624, 698
CviQI GTAC 4 cut(s) 303, 474, 684, 726
DdeI CTNAG 1 cut(s) 134
DpnI GATC 2 cut(s) 297, 517
DpnII GATC 2 cut(s) 295, 515
EaeI YGGCCR 1 cut(s) 191
Eam1104I CTCTTC 3 cut(s) 210, 365, 446
EarI CTCTTC 3 cut(s) 210, 365, 446
Ecl136II GAGCTC 1 cut(s) 436
Eco130I CCWWGG 1 cut(s) 443
Eco24I GRGCYC 1 cut(s) 438
Eco31I GGTCTC 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 74
Eco47III AGCGCT 1 cut(s) 595
Eco53kI GAGCTC 1 cut(s) 436
Eco88I CYCGRG 1 cut(s) 386
Eco91I GGTNACC 1 cut(s) 338
EcoICRI GAGCTC 1 cut(s) 436
EcoO65I GGTNACC 1 cut(s) 338
EcoT14I CCWWGG 1 cut(s) 443
EcoT38I GRGCYC 1 cut(s) 438
ErhI CCWWGG 1 cut(s) 443
FaeI CATG 3 cut(s) 83, 159, 192
FalI AAGNNNNNCTT 2 cut(s) 250, 282
FatI CATG 3 cut(s) 79, 155, 188
FokI GGATG 1 cut(s) 193
FriOI GRGCYC 1 cut(s) 438
FspBI CTAG 1 cut(s) 117
GlaI GCGC 1 cut(s) 595
HaeII RGCGCY 1 cut(s) 597
HaeIII GGCC 2 cut(s) 193, 624
HapII CCGG 2 cut(s) 584, 681
HhaI GCGC 1 cut(s) 596
Hin1II CATG 3 cut(s) 83, 159, 192
Hin6I GCGC 1 cut(s) 594
HinP1I GCGC 1 cut(s) 594
HindIII AAGCTT 4 cut(s) 5, 68, 253, 565
HinfI GANTC 6 cut(s) 202, 328, 373, 391, 524, 709
HpaII CCGG 2 cut(s) 584, 681
HphI GGTGA 1 cut(s) 598
Hpy166II GTNNAC 2 cut(s) 303, 305
Hpy188I TCNGA 2 cut(s) 283, 515
Hpy188III TCNNGA 6 cut(s) 38, 80, 388, 431, 706, 713
Hpy8I GTNNAC 2 cut(s) 303, 305
HpyAV CCTTC 1 cut(s) 688
HpyCH4III ACNGT 3 cut(s) 86, 94, 141
HpyCH4IV ACGT 2 cut(s) 241, 276
HpyCH4V TGCA 2 cut(s) 273, 411
HpyF3I CTNAG 1 cut(s) 134
HpySE526I ACGT 2 cut(s) 241, 276
Hsp92II CATG 3 cut(s) 83, 159, 192
HspAI GCGC 1 cut(s) 594
Kzo9I GATC 2 cut(s) 295, 515
LmnI GCTCC 2 cut(s) 441, 703
MaeI CTAG 1 cut(s) 117
MaeII ACGT 2 cut(s) 241, 276
MaeIII GTNAC 1 cut(s) 338
MalI GATC 2 cut(s) 297, 517
MboI GATC 2 cut(s) 295, 515
MboII GAAGA 4 cut(s) 197, 382, 419, 463
MhlI GDGCHC 1 cut(s) 438
MluCI AATT 5 cut(s) 345, 406, 423, 496, 631
MlyI GAGTC 3 cut(s) 196, 382, 385
MnlI CCTC 6 cut(s) 24, 34, 87, 277, 428, 447
MroXI GAANNNNTTC 2 cut(s) 48, 426
MseI TTAA 2 cut(s) 257, 491
MslI CAYNNNNRTG 2 cut(s) 84, 605
MspCI CTTAAG 1 cut(s) 256
MspI CCGG 2 cut(s) 584, 681
NdeII GATC 2 cut(s) 295, 515
NlaIII CATG 3 cut(s) 83, 159, 192
NlaIV GGNNCC 2 cut(s) 288, 699
NmeAIII GCCGAG 1 cut(s) 219
PaeR7I CTCGAG 1 cut(s) 386
PagI TCATGA 1 cut(s) 79
PdmI GAANNNNTTC 2 cut(s) 48, 426
PfeI GAWTC 3 cut(s) 328, 524, 709
PinAI ACCGGT 2 cut(s) 583, 680
PleI GAGTC 3 cut(s) 196, 381, 385
PpsI GAGTC 3 cut(s) 196, 381, 385
Psp124BI GAGCTC 1 cut(s) 438
PspEI GGTNACC 1 cut(s) 338
PspN4I GGNNCC 2 cut(s) 288, 699
PspPI GGNCC 2 cut(s) 74, 622
RsaI GTAC 4 cut(s) 304, 475, 685, 727
RsaNI GTAC 4 cut(s) 303, 474, 684, 726
RseI CAYNNNNRTG 2 cut(s) 84, 605
SacI GAGCTC 1 cut(s) 438
SaqAI TTAA 2 cut(s) 257, 491
Sau3AI GATC 2 cut(s) 295, 515
Sau96I GGNCC 2 cut(s) 74, 622
SchI GAGTC 3 cut(s) 196, 382, 385
SduI GDGCHC 1 cut(s) 438
Sfr274I CTCGAG 1 cut(s) 386
SinI GGWCC 1 cut(s) 74
SlaI CTCGAG 1 cut(s) 386
SmiMI CAYNNNNRTG 2 cut(s) 84, 605
SmlI CTYRAG 3 cut(s) 38, 256, 386
SmoI CTYRAG 3 cut(s) 38, 256, 386
Sse9I AATT 5 cut(s) 345, 406, 423, 496, 631
SspMI CTAG 1 cut(s) 117
SstI GAGCTC 1 cut(s) 438
StyI CCWWGG 1 cut(s) 443
TaaI ACNGT 3 cut(s) 86, 94, 141
TaiI ACGT 2 cut(s) 244, 279
TaqI TCGA 3 cut(s) 387, 420, 660
TasI AATT 5 cut(s) 345, 406, 423, 496, 631
TatI WGTACW 2 cut(s) 302, 473
TfiI GAWTC 3 cut(s) 328, 524, 709
Tru1I TTAA 2 cut(s) 257, 491
Tru9I TTAA 2 cut(s) 257, 491
TscAI CASTG 3 cut(s) 91, 231, 658
TspDTI ATGAA 7 cut(s) 17, 35, 140, 383, 468, 683, 732
TspRI CASTG 3 cut(s) 91, 231, 658
Vha464I CTTAAG 1 cut(s) 256
VpaK11BI GGWCC 1 cut(s) 74
XapI RAATTY 2 cut(s) 423, 496
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
XhoI CTCGAG 1 cut(s) 386
XmnI GAANNNNTTC 2 cut(s) 48, 426
XspI CTAG 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.