MD12G1024400.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000654
Physical Location & Seq
Forward (+)
2619654 .. 2620604
951 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1024400.v1.1.491

Sequence Viewer

Length: 951 bp
ATGCAGTCCTCATTAGAGTACTTGGATCTTGATGGTAATCGGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAGACTCTTAAAGTATCTGGTTGCAGAAACCTCACAATACTTCCAGAGCTCCCACGTTCTTTGACATGTTTAACACTGGCTTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCAGCCCTCATTAGAGTACTTGGATCTTGATTGTAATCCGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAGACTCTTAAAGTATCTGGTTGCAGAAACCTCACAATACTTCCAGAGCTCCCAAGTTCTTTGACATGTTTAACACTGGCTTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCTGTCCTCGTTGGAGTACTTGGATCTTGATGGTAATCCGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAGACTCTTAAAGTATCTGGTTGCAGAAACCTCACAATACTTCCAGAGCTCCCACGTTCTTTGACATGTTTAACACTGGCTTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCTGTCCTCGTTGGAGTACTTGGATCTTGATGGTAATCCGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAGACTCTTAAAGTATCTGGTTGCAGAAACCTCACAATACTTCCAGAGCTCCCATGTTCTTTGACAAGTTTAACACTAGCTTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCTGTCCTCGTTGGAGAAGTTGGATCTGAGTTGCAATCCGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAAACTCTTAAAGTATCTGGTTGCAGAAACCTCACAATACTTCCAGAGCTCCACATTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

317

Amino Acids

34.31

Weight (kDa)

4.74

Isoelectric Point (pI)

62.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 4 - 46 2.8e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 5 - 64 7.4e-06 Leucine-rich repeat region
LRR_8 PF13855 47 - 104 1.4e-07 Leucine rich repeat
LRR_4 PF12799 48 - 86 6.8e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 115 - 171 4.3e-08 Leucine rich repeat
LRR_4 PF12799 115 - 153 5.4e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 116 - 186 4.4e-08 Leucine-rich repeat region
LRR_8 PF13855 181 - 238 3.4e-08 Leucine rich repeat
LRR_4 PF12799 182 - 220 4.5e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 183 - 253 8.4e-08 Leucine-rich repeat region
LRR_8 PF13855 249 - 305 1.2e-09 Leucine rich repeat
LRR_4 PF12799 249 - 287 1.2e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 251 - 314 9.3e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 5 cut(s) 33, 234, 435, 636, 837
AfaI GTAC 4 cut(s) 20, 221, 422, 623
AflIII ACRYGT 3 cut(s) 149, 350, 551
Alw21I GWGCWC 5 cut(s) 135, 336, 537, 738, 939
AlwI GGATC 5 cut(s) 33, 234, 435, 636, 837
ApeKI GCWGC 5 cut(s) 168, 205, 369, 570, 771
BaeGI GKGCMC 4 cut(s) 196, 397, 598, 799
BanII GRGCYC 5 cut(s) 135, 336, 537, 738, 939
Bbv12I GWGCWC 5 cut(s) 135, 336, 537, 738, 939
BbvI GCAGC 5 cut(s) 155, 217, 356, 557, 758
BccI CCATC 3 cut(s) 26, 428, 629
BfaI CTAG 1 cut(s) 764
BisI GCNGC 5 cut(s) 169, 206, 370, 571, 772
BlsI GCNGC 5 cut(s) 170, 207, 371, 572, 773
BmcAI AGTACT 4 cut(s) 20, 221, 422, 623
BpmI CTGGAG 4 cut(s) 68, 269, 470, 671
BsaBI GATNNNNATC 4 cut(s) 36, 237, 438, 639
Bse1I ACTGG 3 cut(s) 165, 366, 567
Bse8I GATNNNNATC 4 cut(s) 36, 237, 438, 639
BseJI GATNNNNATC 4 cut(s) 36, 237, 438, 639
BseMII CTCAG 1 cut(s) 824
BseNI ACTGG 3 cut(s) 165, 366, 567
BseSI GKGCMC 4 cut(s) 196, 397, 598, 799
BseXI GCAGC 5 cut(s) 155, 217, 356, 557, 758
BsiHKAI GWGCWC 5 cut(s) 135, 336, 537, 738, 939
Bsp1286I GDGCHC 9 cut(s) 135, 196, 336, 397, 537, 598, 738, 799, 939
Bsp143I GATC 5 cut(s) 25, 226, 427, 628, 829
BspCNI CTCAG 1 cut(s) 825
BspPI GGATC 5 cut(s) 33, 234, 435, 636, 837
BsrI ACTGG 3 cut(s) 165, 366, 567
BssMI GATC 5 cut(s) 25, 226, 427, 628, 829
BstC8I GCNNGC 4 cut(s) 166, 367, 568, 769
BstDEI CTNAG 1 cut(s) 833
BstKTI GATC 5 cut(s) 28, 229, 430, 631, 832
BstMBI GATC 5 cut(s) 25, 226, 427, 628, 829
BstNSI RCATGY 3 cut(s) 153, 354, 555
BstSLI GKGCMC 4 cut(s) 196, 397, 598, 799
BstV1I GCAGC 5 cut(s) 155, 217, 356, 557, 758
BstX2I RGATCY 5 cut(s) 25, 226, 427, 628, 829
BstYI RGATCY 5 cut(s) 25, 226, 427, 628, 829
BtsIMutI CAGTG 3 cut(s) 158, 359, 560
Cac8I GCNNGC 4 cut(s) 166, 367, 568, 769
Csp6I GTAC 4 cut(s) 19, 220, 421, 622
CspCI CAANNNNNGTGG 5 cut(s) 126, 161, 528, 563, 929
CviAII CATG 9 cut(s) 64, 150, 265, 351, 466, 552, 667, 741, 868
CviQI GTAC 4 cut(s) 19, 220, 421, 622
DdeI CTNAG 1 cut(s) 833
DpnI GATC 5 cut(s) 27, 228, 429, 630, 831
DpnII GATC 5 cut(s) 25, 226, 427, 628, 829
Ecl136II GAGCTC 5 cut(s) 133, 334, 535, 736, 937
Eco24I GRGCYC 5 cut(s) 135, 336, 537, 738, 939
Eco53kI GAGCTC 5 cut(s) 133, 334, 535, 736, 937
EcoICRI GAGCTC 5 cut(s) 133, 334, 535, 736, 937
EcoT38I GRGCYC 5 cut(s) 135, 336, 537, 738, 939
FaeI CATG 9 cut(s) 67, 153, 268, 354, 469, 555, 670, 744, 871
FatI CATG 9 cut(s) 63, 149, 264, 350, 465, 551, 666, 740, 867
Fnu4HI GCNGC 5 cut(s) 169, 206, 370, 571, 772
FriOI GRGCYC 5 cut(s) 135, 336, 537, 738, 939
Fsp4HI GCNGC 5 cut(s) 169, 206, 370, 571, 772
FspBI CTAG 1 cut(s) 764
GluI GCNGC 5 cut(s) 169, 206, 370, 571, 772
GsuI CTGGAG 4 cut(s) 68, 269, 470, 671
Hin1II CATG 9 cut(s) 67, 153, 268, 354, 469, 555, 670, 744, 871
HinfI GANTC 8 cut(s) 88, 184, 289, 385, 490, 586, 691, 787
HpyCH4IV ACGT 2 cut(s) 139, 541
HpyF3I CTNAG 1 cut(s) 833
HpySE526I ACGT 2 cut(s) 139, 541
Hsp92II CATG 9 cut(s) 67, 153, 268, 354, 469, 555, 670, 744, 871
Kzo9I GATC 5 cut(s) 25, 226, 427, 628, 829
Lsp1109I GCAGC 5 cut(s) 155, 217, 356, 557, 758
MaeI CTAG 1 cut(s) 764
MaeII ACGT 2 cut(s) 139, 541
MalI GATC 5 cut(s) 27, 228, 429, 630, 831
MboI GATC 5 cut(s) 25, 226, 427, 628, 829
MflI RGATCY 5 cut(s) 25, 226, 427, 628, 829
MhlI GDGCHC 9 cut(s) 135, 196, 336, 397, 537, 598, 738, 799, 939
MlyI GAGTC 4 cut(s) 82, 283, 484, 685
MmeI TCCRAC 4 cut(s) 396, 597, 798, 807
NdeII GATC 5 cut(s) 25, 226, 427, 628, 829
NlaIII CATG 9 cut(s) 67, 153, 268, 354, 469, 555, 670, 744, 871
NspI RCATGY 3 cut(s) 153, 354, 555
PciI ACATGT 3 cut(s) 149, 350, 551
PfeI GAWTC 4 cut(s) 184, 385, 586, 787
PkrI GCNGC 5 cut(s) 170, 207, 371, 572, 773
PleI GAGTC 4 cut(s) 82, 283, 484, 685
PpsI GAGTC 4 cut(s) 82, 283, 484, 685
PscI ACATGT 3 cut(s) 149, 350, 551
Psp124BI GAGCTC 5 cut(s) 135, 336, 537, 738, 939
PsuI RGATCY 5 cut(s) 25, 226, 427, 628, 829
RsaI GTAC 4 cut(s) 20, 221, 422, 623
RsaNI GTAC 4 cut(s) 19, 220, 421, 622
SacI GAGCTC 5 cut(s) 135, 336, 537, 738, 939
SatI GCNGC 5 cut(s) 169, 206, 370, 571, 772
Sau3AI GATC 5 cut(s) 25, 226, 427, 628, 829
ScaI AGTACT 4 cut(s) 20, 221, 422, 623
SchI GAGTC 4 cut(s) 82, 283, 484, 685
SduI GDGCHC 9 cut(s) 135, 196, 336, 397, 537, 598, 738, 799, 939
SspMI CTAG 1 cut(s) 764
SstI GAGCTC 5 cut(s) 135, 336, 537, 738, 939
TaiI ACGT 2 cut(s) 142, 544
TatI WGTACW 4 cut(s) 18, 219, 420, 621
TfiI GAWTC 4 cut(s) 184, 385, 586, 787
TscAI CASTG 3 cut(s) 165, 366, 567
TseI GCWGC 5 cut(s) 168, 205, 369, 570, 771
TspDTI ATGAA 5 cut(s) 80, 281, 482, 683, 884
TspRI CASTG 3 cut(s) 165, 366, 567
XceI RCATGY 3 cut(s) 153, 354, 555
XspI CTAG 1 cut(s) 764
ZrmI AGTACT 4 cut(s) 20, 221, 422, 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.