MD07G1079200.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000654
Physical Location & Seq
Forward (+)
7629485 .. 7634196
4712 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1079200.v1.1.491

Sequence Viewer

Length: 3645 bp
ATGGCTGGTGCTTCAAGCTCCATGCAGCTGCACGACACTTCTTCACGCTCTTACCGATGTACTTATCATGCGTTCTTGAGTTTCAGAGGGGCAGATACGCGTAAGAGCTTTACTGATCACCTCTACCGGGCTTTGGAGCTGGCAGGAATCCACACCTTTAGAGACGATGATGAAATCAAGAGAGGAGAAGATATAGCAGCAGAGTTACATAATGCTATAAATGAGTCAAAAGTATCGATAATTGTCTTCTCCAAGGACTATGCTTCATCGCGGTGGTGCTTGGACGAACTTGTTAAGATCATGGAACGTAGAAGAAGTGATGATGGACACTTTGTTTTGCCGGTTTTCTATGATGTGGATCCGTCCCATGTGAGGAAGCAGACTGCGAGTTTCGAAGAATCATTTTGTAGACATGAAGAGCGCTTCAAGGAGGAGATGGACAAGGTGGAGGGGTGGAGAAGAGCACTTAAAGATGTTGCAGATCTGGCAGGGATGGTTTTGGAAAATAGGTACGAGTCGCAGTTTATCCAAGATATTGTTAAAGAGATCGGAAGTAAATTGGATCCCAAAGCATTGAACGTTGCACCGTATGCTGTTGGCATAGACGATCGTGTACGAGGCATAAACATGTGGTTACAAGACGGATCAAATGATGTTGGTATAGCTGTGATATGTGGAATGGGTGGAATTGGCAAGACCACCATTGCCAGAGCTGCCTATAACCTCAATTATGCAAGATTCCAAGGTAGCAGCTTTCTTGCAGATATTAGAGCAAATTCAGAACAGCCATATGGTCTCGTTCGCTTGCAAAGGAAACTTCTTTCGGACATCCAAAAATGGAAAGCAGATAAAATATACGACATAAATGAAGGGATAATGAAGATCAAACATGCTGTCTGTAACAAGAGAGTTCTTATTGTTCTTGACGATGTGAATGACTTGGATCAATTTAACGCAATTCTTGGAATGCGGGAATGGTTTTATCCAGGAAGTAAAATCATCGTAACAACCAGGCACGAGCATTTGTTGAAGGCACATGAAGATAATGCAATGTTCGAGGTTGAAGAATTGAATGAGGATGAATCGCTTGAGCTTTTCAGTTGGCATGCATTTGGACAAGCCCATCCTATTGAAGGTTACATGGAACTTTCAAGACCAGCAGTGCAACACTGTGAAGGGCTACCCTTAGCTCTTCAAGTTTTGGGATCCTCTCTATCTGGGAAAGCAGTAGATGTATGGAAAAGTGCTTTACAGAAGTTATGCGAAATTCCTGATGACAAAGTTCACAAAGTTCTTCGAGTAAGCTATGATTCTCTACAAGATGATAATGACAAAAATGTATTCCTCTATATAGCCGGTTTCTTCATTGGAAAGGAGAAGGATTTTGCAATTACAGTACTGGACAACCTTGATTTTTACACGAGGATTGCAATTCAAAATCTGGTGGACAGATGTCTAGTAAAAATCAATAATGAAGACAACAGGCTGGCCATGCATCACCTACTTAGAGACATGGGAAGGGGAATTATTCGTGAAGAATCACCTCAAGATCCCGGAAAGCGTAGGATAGTGTGGCATAAAGATTCCTTTAATATTTTGAGAAAAAGAACGGGTACGGCAACTGTTAAGGGCCTCATGCTCAACCTGCCTGTGTTGATGGAAGATGAGTCTTTGAAGCCATTATTTGGTAAAAGTAACAAAAAACGATGCAATGTTGAAGATTATGAAGGAAAATTTTCAAGACGTCGTCGGCTTGATTTCTTTTCTTGGAAATCAATTGCAAGTAACTTTTCTTCAACAAACTCAACTCCCGCATCAAATGATTTGAAGTTCAGAACTGACGCATTTACAATGATGAACAATCTTGAACTGCTCCTGCTCAATAATGTAAGCATCTGTGGAGGCTTTGAAGATTTTCCAAAATATTTGACATGGTTGTCTTGGAGAGGGTTTCCTTTAAAATCGATACCAGCCAATTTTTGTTTGGAAAATCTAGTTGTTCTTGACTTGCGGAATAGCAGCCTGCAACATGTTTGGAAGGGAACCAGGTTTCTTGGCAGAATGAAAGTTCTTAATCTCAGTCATTCACACAGCCTTTTGACAACCCCTGACATGTCGGGACTTCCTAACCTTGAGAGATTAATTCTTAAAGATTGTATAAGTTTGGTTGAGGTTGATGAGTCTATAGGAGACTTGGAGAAACTTCTTGTTTTGAATCTAAGAGACTGCAAAAATCTCAGTAAGATTCCAAGTTTGAGTAGGTCAGGATCTCTTCAAGTTCTCATTCTTTCTGGTTGCTCAAAGCTCGGTAATGCAACGGAGCAGGTCTACTCAGGAGCTAGTGTTATGAAGAAATTCAATCTGTTATCAGCTAACTTATGGCAATCATTAACGTCGTGGGTATTACCAAGAAAAAATCTTGCATTGACCAGTTTCTCATTCAAAAGTATACCACATTCTTTAGGAAGATTAAGTCTTGCCAACTGCAATCTATCAGAGATTTCTAGTGATCTTGGTGTCCTATCGGCGTTGAAGCATTTGGATCTATCTGCAAACCCAATTCCAAACCTACCGGAAAACATGAACGGTCTTCACATGCTCCAAGAACTGCTGGTAGAGGGTTGCACAAAGCTCCAAATGCTTCCGGAGCTTCCACCAAAATTAAAGACATTGGAAGCAGGTCGGTGTACATCATTGAAAAGAATAACAAATTTACCAAACGCTTTCGAATCATTGAGTAAAAATTTTGAGGAATGTGAGAAGTTAGATGAAATTCAAAGCTTGTTCGAAATAAAACCGTTGAGAAACGTTGACATAGAAATGATCAAAGATGTGGGCCTGTTTAACTTGGAATCCACTGAAAGCACCGAGGTCGAAATGATCAACTACCTGACATATACAACCAAGAAGTGTTGCCTTCAGGTACTGAAAGAATGCGGCATATTTAGCATTTTCATTCATGGGAGCAAAATTCCAGATTGGTTCAGCTACAGGAGCATGGGTAACTCGTTGTTGTCGATTATCGTACCTTCACATCTTAATATCAAGGTCCGAGGCTTAAATGCATGCGTTGTATACACACGACTCCCTGATCGTAAGGGTGGTGTACATGTTGCCAGTGAACACTTTCTCAAAGTGAGTAATGAAACCAAGGGTCGTATGTGGACCTATTCCCCTGTCGCAATGGGTCTTCCAAAAGAGAACGAAGACATGCTATGGCTAAGTCATTGGGTGTTTGGAAGTGATGAACTGGAGAGTGGGGACGAAATACGGGTTTCTGTGAAGTGCGGTTTGTGGATAAAAGAGTTTGGAATCGAACTTGCGTACAAGGACGAAGATAAGGGTGAGGGTGTTCGATCAAATAATGAAGATACAACACTTCCTTGGAGCCAAAGTAACGTTGTTGCTGGAGACGTGACAGTGTCGGATTCAATGTACGAAATGTGGAGAGGCAAATACTTTCTTTGTAATCATAGGTTAAGAGCCCATCAAGCTCAATTCAGAAGGAGACAAGAGAACCCAGCTTTTGGTGATTTTTCATACAAGCCAGGGGACTCCTTTTATTTGAATGGTTTCTTATTCAAGCACGAGGTTGACATTGCTGAGTACCAACTGCAGTATTTTAGAAAAGCATTGACTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1215

Amino Acids

138.23

Weight (kDa)

6.58

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 189 7.9e-52 TIR domain
TIR_2 PF13676 25 - 130 1.1e-16 TIR domain
NB-ARC PF00931 203 - 371 6.6e-21 NB-ARC domain
WHD_ROQ1 PF23282 442 - 514 2.4e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 824 - 964 1.4e-06 Leucine-rich repeat region
C-JID PF20160 987 - 1113 1.6e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1936
AatII GACGTC 1 cut(s) 1747
Acc36I ACCTGC 3 cut(s) 1653, 2314, 2669
AccB7I CCANNNNNTGG 2 cut(s) 1685, 3527
AccI GTMKAC 4 cut(s) 409, 2328, 2449, 3075
AccII CGCG 2 cut(s) 100, 271
AccIII TCCGGA 1 cut(s) 2644
AciI CCGC 6 cut(s) 271, 970, 1812, 2011, 2937, 3288
AclI AACGTT 3 cut(s) 579, 2808, 3399
AcoI YGGCCR 1 cut(s) 1488
AcsI RAATTY 8 cut(s) 775, 1266, 1733, 2354, 2710, 2743, 2772, 2970
AcuI CTGAAG 1 cut(s) 2903
AcyI GRCGYC 1 cut(s) 1744
AfeI AGCGCT 1 cut(s) 422
AfiI CCNNNNNNNGG 6 cut(s) 127, 133, 372, 1133, 1685, 3527
AflIII ACRYGT 5 cut(s) 98, 627, 2029, 2112, 3109
AjiI CACGTC 1 cut(s) 3415
AjnI CCWGG 4 cut(s) 985, 1010, 2045, 3547
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 7 cut(s) 156, 800, 1503, 2184, 2217, 3405, 3502
AlwNI CAGNNNCTG 1 cut(s) 2926
Aor13HI TCCGGA 1 cut(s) 2644
Aor51HI AGCGCT 1 cut(s) 422
AoxI GGCC 3 cut(s) 1488, 1630, 2836
ApeKI GCWGC 6 cut(s) 25, 28, 197, 713, 750, 2019
ApoI RAATTY 8 cut(s) 775, 1266, 1733, 2354, 2710, 2743, 2772, 2970
ArsI GACNNNNNNTTYG 2 cut(s) 879, 911
AseI ATTAAT 1 cut(s) 2141
Asp700I GAANNNNTTC 5 cut(s) 1735, 1914, 2354, 3126, 3572
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 4 cut(s) 1630, 2836, 3049, 3165
AsuC2I CCSGG 2 cut(s) 128, 1554
AsuHPI GGTGA 5 cut(s) 110, 1490, 1533, 3356, 3542
AsuII TTCGAA 3 cut(s) 393, 2727, 2787
AvaII GGWCC 2 cut(s) 3049, 3165
BalI TGGCCA 1 cut(s) 1490
BamHI GGATCC 3 cut(s) 358, 562, 1205
BanII GRGCYC 1 cut(s) 3487
BarI GAAGNNNNNNTAC 2 cut(s) 1777, 1809
BauI CACGAG 3 cut(s) 1016, 1420, 3587
BbsI GAAGAC 5 cut(s) 238, 1482, 2582, 3182, 3213
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 6 cut(s) 15, 37, 209, 700, 762, 2031
BccI CCATC 6 cut(s) 317, 430, 487, 1131, 1651, 3495
BceAI ACGGC 1 cut(s) 1632
BcgI CGANNNNNNTGC 2 cut(s) 2854, 2888
BciT130I CCWGG 4 cut(s) 987, 1012, 2047, 3549
BclI TGATCA 3 cut(s) 115, 2823, 2880
BcnI CCSGG 2 cut(s) 128, 1554
BcoDI GTCTC 7 cut(s) 156, 800, 1503, 2184, 2217, 3405, 3502
BfaI CTAG 4 cut(s) 1457, 1994, 2340, 2505
BfmI CTRYAG 3 cut(s) 2184, 2989, 3614
BfoI RGCGCY 1 cut(s) 424
BfuAI ACCTGC 3 cut(s) 1653, 2314, 2669
BglII AGATCT 1 cut(s) 481
BisI GCNGC 7 cut(s) 26, 29, 198, 714, 751, 2020, 2938
BlsI GCNGC 7 cut(s) 27, 30, 199, 715, 752, 2021, 2939
BmcAI AGTACT 1 cut(s) 1398
Bme1390I CCNGG 6 cut(s) 128, 987, 1012, 1554, 2047, 3549
Bme18I GGWCC 2 cut(s) 3049, 3165
BmgBI CACGTC 1 cut(s) 3415
BmgT120I GGNCC 4 cut(s) 1630, 2836, 3049, 3165
BmiI GGNNCC 5 cut(s) 360, 564, 1207, 2044, 3389
BmrFI CCNGG 6 cut(s) 128, 987, 1012, 1554, 2047, 3549
BmsI GCATC 4 cut(s) 1504, 1697, 1823, 1902
BoxI GACNNNNGTC 1 cut(s) 1452
BpiI GAAGAC 5 cut(s) 238, 1482, 2582, 3182, 3213
BpmI CTGGAG 2 cut(s) 3272, 3429
Bpu10I CCTNAGC 1 cut(s) 1186
Bpu14I TTCGAA 3 cut(s) 393, 2727, 2787
BpuEI CTTGAG 4 cut(s) 97, 1109, 1530, 2153
BpuMI CCSGG 2 cut(s) 128, 1554
Bsa29I ATCGAT 2 cut(s) 236, 1964
BsaBI GATNNNNATC 1 cut(s) 357
BsaHI GRCGYC 1 cut(s) 1744
BsaI GGTCTC 1 cut(s) 800
BsaJI CCNNGG 7 cut(s) 252, 742, 2868, 3052, 3150, 3383, 3548
BsaWI WCCGGW 2 cut(s) 2572, 2644
BsaXI ACNNNNNCTCC 6 cut(s) 2328, 2358, 2986, 3016, 3379, 3409
Bsc4I CCNNNNNNNGG 6 cut(s) 127, 133, 372, 1133, 1685, 3527
Bse118I RCCGGY 2 cut(s) 340, 1355
Bse1I ACTGG 4 cut(s) 1404, 2430, 3117, 3255
Bse3DI GCAATG 5 cut(s) 702, 1056, 1717, 3189, 3597
Bse8I GATNNNNATC 1 cut(s) 357
BseAI TCCGGA 1 cut(s) 2644
BseBI CCWGG 4 cut(s) 987, 1012, 2047, 3549
BseCI ATCGAT 2 cut(s) 236, 1964
BseDI CCNNGG 7 cut(s) 252, 742, 2868, 3052, 3150, 3383, 3548
BseGI GGATG 4 cut(s) 498, 828, 1084, 1123
BseJI GATNNNNATC 1 cut(s) 357
BseLI CCNNNNNNNGG 6 cut(s) 127, 133, 372, 1133, 1685, 3527
BseMI GCAATG 5 cut(s) 702, 1056, 1717, 3189, 3597
BseMII CTCAG 4 cut(s) 2092, 2251, 2346, 3594
BseNI ACTGG 4 cut(s) 1404, 2430, 3117, 3255
BseRI GAGGAG 2 cut(s) 198, 446
BseXI GCAGC 6 cut(s) 15, 37, 209, 700, 762, 2031
BseYI CCCAGC 1 cut(s) 3520
BsgI GTGCAG 1 cut(s) 14
Bsh1236I CGCG 2 cut(s) 100, 271
Bsh1285I CGRYCG 1 cut(s) 610
BshFI GGCC 3 cut(s) 1490, 1632, 2838
BshVI ATCGAT 2 cut(s) 236, 1964
BsiEI CGRYCG 1 cut(s) 610
BsiHKAI GWGCWC 1 cut(s) 466
BsiSI CCGG 6 cut(s) 127, 341, 1356, 1554, 2573, 2645
BslFI GGGAC 4 cut(s) 349, 2133, 3275, 3566
BslI CCNNNNNNNGG 6 cut(s) 127, 133, 372, 1133, 1685, 3527
BsmAI GTCTC 7 cut(s) 156, 800, 1503, 2184, 2217, 3405, 3502
BsmBI CGTCTC 2 cut(s) 156, 3405
BsmFI GGGAC 4 cut(s) 349, 2133, 3275, 3566
BsmI GAATGC 2 cut(s) 972, 2939
BsnI GGCC 3 cut(s) 1490, 1632, 2838
Bso31I GGTCTC 1 cut(s) 800
Bsp119I TTCGAA 3 cut(s) 393, 2727, 2787
Bsp1286I GDGCHC 2 cut(s) 466, 3487
Bsp13I TCCGGA 1 cut(s) 2644
Bsp1407I TGTACA 2 cut(s) 2687, 3106
BspACI CCGC 6 cut(s) 271, 970, 1812, 2011, 2937, 3288
BspANI GGCC 3 cut(s) 1490, 1632, 2838
BspCNI CTCAG 4 cut(s) 2091, 2250, 2345, 3595
BspDI ATCGAT 2 cut(s) 236, 1964
BspEI TCCGGA 1 cut(s) 2644
BspFNI CGCG 2 cut(s) 100, 271
BspLI GGNNCC 5 cut(s) 360, 564, 1207, 2044, 3389
BspMAI CTGCAG 1 cut(s) 3618
BspMI ACCTGC 3 cut(s) 1653, 2314, 2669
BspQI GCTCTTC 3 cut(s) 411, 454, 1197
BspT104I TTCGAA 3 cut(s) 393, 2727, 2787
BspTNI GGTCTC 1 cut(s) 800
BsrDI GCAATG 5 cut(s) 702, 1056, 1717, 3189, 3597
BsrFI RCCGGY 2 cut(s) 340, 1355
BsrGI TGTACA 2 cut(s) 2687, 3106
BsrI ACTGG 4 cut(s) 1404, 2430, 3117, 3255
BssAI RCCGGY 2 cut(s) 340, 1355
BssECI CCNNGG 7 cut(s) 252, 742, 2868, 3052, 3150, 3383, 3548
BssNAI GTATAC 2 cut(s) 2450, 3076
BssNI GRCGYC 1 cut(s) 1744
BssSI CACGAG 3 cut(s) 1016, 1420, 3587
BssT1I CCWWGG 4 cut(s) 252, 742, 3150, 3383
Bst1107I GTATAC 2 cut(s) 2450, 3076
Bst2BI CACGAG 3 cut(s) 1016, 1420, 3587
Bst2UI CCWGG 4 cut(s) 987, 1012, 2047, 3549
Bst4CI ACNGT 7 cut(s) 588, 1172, 1396, 1624, 2588, 2799, 3421
Bst6I CTCTTC 4 cut(s) 411, 454, 1197, 2277
BstACI GRCGYC 1 cut(s) 1744
BstAPI GCANNNNNTGC 1 cut(s) 590
BstAUI TGTACA 2 cut(s) 2687, 3106
BstBI TTCGAA 3 cut(s) 393, 2727, 2787
BstC8I GCNNGC 6 cut(s) 141, 806, 1107, 1488, 2024, 3067
BstDEI CTNAG 8 cut(s) 1186, 1505, 2078, 2219, 2237, 2332, 3221, 3603
BstENI CCTNNNNNAGG 1 cut(s) 1131
BstF5I GGATG 4 cut(s) 498, 828, 1084, 1123
BstFNI CGCG 2 cut(s) 100, 271
BstH2I RGCGCY 1 cut(s) 424
BstHHI GCGC 1 cut(s) 423
BstMAI GTCTC 7 cut(s) 156, 800, 1503, 2184, 2217, 3405, 3502
BstMCI CGRYCG 1 cut(s) 610
BstNI CCWGG 4 cut(s) 987, 1012, 2047, 3549
BstNSI RCATGY 9 cut(s) 631, 893, 1109, 2033, 2116, 2599, 3069, 3113, 3214
BstPAI GACNNNNGTC 1 cut(s) 1452
BstSCI CCNGG 6 cut(s) 126, 985, 1010, 1552, 2045, 3547
BstSFI CTRYAG 3 cut(s) 2184, 2989, 3614
BstUI CGCG 2 cut(s) 100, 271
BstV1I GCAGC 6 cut(s) 15, 37, 209, 700, 762, 2031
BstV2I GAAGAC 5 cut(s) 238, 1482, 2582, 3182, 3213
BstX2I RGATCY 7 cut(s) 358, 481, 562, 1205, 1549, 2267, 2542
BstYI RGATCY 7 cut(s) 358, 481, 562, 1205, 1549, 2267, 2542
BstZ17I GTATAC 2 cut(s) 2450, 3076
Bsu15I ATCGAT 2 cut(s) 236, 1964
BsuRI GGCC 3 cut(s) 1490, 1632, 2838
BsuTUI ATCGAT 2 cut(s) 236, 1964
BtgZI GCGATG 1 cut(s) 252
BtrI CACGTC 1 cut(s) 3415
BtsCI GGATG 4 cut(s) 498, 828, 1084, 1123
BtsI GCAGTG 1 cut(s) 1167
BtsIMutI CAGTG 5 cut(s) 1167, 1168, 2856, 3124, 3426
BveI ACCTGC 3 cut(s) 1653, 2314, 2669
Cac8I GCNNGC 6 cut(s) 141, 806, 1107, 1488, 2024, 3067
CaiI CAGNNNCTG 1 cut(s) 2926
CfoI GCGC 1 cut(s) 423
Cfr10I RCCGGY 2 cut(s) 340, 1355
Cfr13I GGNCC 4 cut(s) 1630, 2836, 3049, 3165
ClaI ATCGAT 2 cut(s) 236, 1964
CseI GACGC 1 cut(s) 1850
CsiI ACCWGGT 1 cut(s) 2045
DdeI CTNAG 8 cut(s) 1186, 1505, 2078, 2219, 2237, 2332, 3221, 3603
DraI TTTAAA 1 cut(s) 1959
DrdI GACNNNNNNGTC 1 cut(s) 1936
DseDI GACNNNNNNGTC 1 cut(s) 1936
EaeI YGGCCR 1 cut(s) 1488
Eam1104I CTCTTC 4 cut(s) 411, 454, 1197, 2277
EarI CTCTTC 4 cut(s) 411, 454, 1197, 2277
Eco130I CCWWGG 4 cut(s) 252, 742, 3150, 3383
Eco24I GRGCYC 1 cut(s) 3487
Eco31I GGTCTC 1 cut(s) 800
Eco47I GGWCC 2 cut(s) 3049, 3165
Eco47III AGCGCT 1 cut(s) 422
Eco57I CTGAAG 1 cut(s) 2903
EcoNI CCTNNNNNAGG 1 cut(s) 1131
EcoO109I RGGNCCY 1 cut(s) 1630
EcoRII CCWGG 4 cut(s) 985, 1010, 2045, 3547
EcoT14I CCWWGG 4 cut(s) 252, 742, 3150, 3383
EcoT22I ATGCAT 3 cut(s) 1111, 1497, 3067
EcoT38I GRGCYC 1 cut(s) 3487
ErhI CCWWGG 4 cut(s) 252, 742, 3150, 3383
Esp3I CGTCTC 2 cut(s) 156, 3405
FaqI GGGAC 4 cut(s) 349, 2133, 3275, 3566
FauI CCCGC 2 cut(s) 963, 1819
FauNDI CATATG 1 cut(s) 790
FbaI TGATCA 3 cut(s) 115, 2823, 2880
FblI GTMKAC 4 cut(s) 409, 2328, 2449, 3075
Fnu4HI GCNGC 7 cut(s) 26, 29, 198, 714, 751, 2020, 2938
FokI GGATG 4 cut(s) 505, 815, 1091, 1110
FriOI GRGCYC 1 cut(s) 3487
Fsp4HI GCNGC 7 cut(s) 26, 29, 198, 714, 751, 2020, 2938
FspBI CTAG 4 cut(s) 1457, 1994, 2340, 2505
GlaI GCGC 1 cut(s) 422
GluI GCNGC 7 cut(s) 26, 29, 198, 714, 751, 2020, 2938
GsaI CCCAGC 1 cut(s) 3524
GsuI CTGGAG 2 cut(s) 3272, 3429
HaeII RGCGCY 1 cut(s) 424
HaeIII GGCC 3 cut(s) 1490, 1632, 2838
HapII CCGG 6 cut(s) 127, 341, 1356, 1554, 2573, 2645
HgaI GACGC 1 cut(s) 1850
HhaI GCGC 1 cut(s) 423
Hin1I GRCGYC 1 cut(s) 1744
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HincII GTYRAC 2 cut(s) 2812, 3595
HindII GTYRAC 2 cut(s) 2812, 3595
HindIII AAGCTT 1 cut(s) 2779
HpaII CCGG 6 cut(s) 127, 341, 1356, 1554, 2573, 2645
HphI GGTGA 5 cut(s) 110, 1490, 1533, 3356, 3542
Hpy188I TCNGA 9 cut(s) 86, 551, 781, 826, 1835, 2497, 3053, 3427, 3503
Hpy99I CGWCG 3 cut(s) 1749, 1752, 2398
HpyCH4III ACNGT 7 cut(s) 588, 1172, 1396, 1624, 2588, 2799, 3421
HpyCH4IV ACGT 7 cut(s) 307, 579, 1744, 2393, 2808, 3399, 3414
HpyF3I CTNAG 8 cut(s) 1186, 1505, 2078, 2219, 2237, 2332, 3221, 3603
HpySE526I ACGT 7 cut(s) 307, 579, 1744, 2393, 2808, 3399, 3414
Hsp92I GRCGYC 1 cut(s) 1744
HspAI GCGC 1 cut(s) 421
Kpn2I TCCGGA 1 cut(s) 2644
Ksp22I TGATCA 3 cut(s) 115, 2823, 2880
LguI GCTCTTC 3 cut(s) 411, 454, 1197
Lsp1109I GCAGC 6 cut(s) 15, 37, 209, 700, 762, 2031
LweI GCATC 4 cut(s) 1504, 1697, 1823, 1902
MabI ACCWGGT 1 cut(s) 2045
MaeI CTAG 4 cut(s) 1457, 1994, 2340, 2505
MaeII ACGT 7 cut(s) 307, 579, 1744, 2393, 2808, 3399, 3414
MfeI CAATTG 1 cut(s) 1776
MflI RGATCY 7 cut(s) 358, 481, 562, 1205, 1549, 2267, 2542
MhlI GDGCHC 2 cut(s) 466, 3487
MlsI TGGCCA 1 cut(s) 1490
MluI ACGCGT 1 cut(s) 98
MluNI TGGCCA 1 cut(s) 1490
MlyI GAGTC 6 cut(s) 233, 524, 1676, 2189, 3078, 3548
MmeI TCCRAC 1 cut(s) 3405
Mox20I TGGCCA 1 cut(s) 1490
Mph1103I ATGCAT 3 cut(s) 1111, 1497, 3067
MroI TCCGGA 1 cut(s) 2644
MroXI GAANNNNTTC 5 cut(s) 1735, 1914, 2354, 3126, 3572
MscI TGGCCA 1 cut(s) 1490
MslI CAYNNNNRTG 3 cut(s) 271, 626, 2819
Msp20I TGGCCA 1 cut(s) 1490
MspA1I CMGCKG 1 cut(s) 28
MspI CCGG 6 cut(s) 127, 341, 1356, 1554, 2573, 2645
MspR9I CCNGG 6 cut(s) 128, 987, 1012, 1554, 2047, 3549
MunI CAATTG 1 cut(s) 1776
Mva1269I GAATGC 2 cut(s) 972, 2939
MvaI CCWGG 4 cut(s) 987, 1012, 2047, 3549
MvnI CGCG 2 cut(s) 100, 271
NciI CCSGG 2 cut(s) 128, 1554
NdeI CATATG 1 cut(s) 790
NlaIV GGNNCC 5 cut(s) 360, 564, 1207, 2044, 3389
NmuCI GTSAC 1 cut(s) 3415
NsiI ATGCAT 3 cut(s) 1111, 1497, 3067
NspI RCATGY 9 cut(s) 631, 893, 1109, 2033, 2116, 2599, 3069, 3113, 3214
NspV TTCGAA 3 cut(s) 393, 2727, 2787
PaeI GCATGC 2 cut(s) 1109, 3069
PciI ACATGT 4 cut(s) 627, 2029, 2112, 3109
PciSI GCTCTTC 3 cut(s) 411, 454, 1197
PcsI WCGNNNNNNNCGW 2 cut(s) 52, 3014
PctI GAATGC 2 cut(s) 972, 2939
PdmI GAANNNNTTC 5 cut(s) 1735, 1914, 2354, 3126, 3572
PflFI GACNNNGTC 2 cut(s) 1746, 3421
PflMI CCANNNNNTGG 2 cut(s) 1685, 3527
PfoI TCCNGGA 2 cut(s) 985, 1552
PkrI GCNGC 7 cut(s) 27, 30, 199, 715, 752, 2021, 2939
Ple19I CGATCG 1 cut(s) 610
PleI GAGTC 6 cut(s) 232, 523, 1675, 2188, 3078, 3548
PpsI GAGTC 6 cut(s) 232, 523, 1675, 2188, 3078, 3548
PscI ACATGT 4 cut(s) 627, 2029, 2112, 3109
PshAI GACNNNNGTC 1 cut(s) 1452
PshBI ATTAAT 1 cut(s) 2141
Psp1406I AACGTT 3 cut(s) 579, 2808, 3399
Psp6I CCWGG 4 cut(s) 985, 1010, 2045, 3547
PspFI CCCAGC 1 cut(s) 3520
PspGI CCWGG 4 cut(s) 985, 1010, 2045, 3547
PspN4I GGNNCC 5 cut(s) 360, 564, 1207, 2044, 3389
PspPI GGNCC 4 cut(s) 1630, 2836, 3049, 3165
PstI CTGCAG 1 cut(s) 3618
PstNI CAGNNNCTG 1 cut(s) 2926
PsuI RGATCY 7 cut(s) 358, 481, 562, 1205, 1549, 2267, 2542
PsyI GACNNNGTC 2 cut(s) 1746, 3421
PvuI CGATCG 1 cut(s) 610
PvuII CAGCTG 1 cut(s) 28
RseI CAYNNNNRTG 3 cut(s) 271, 626, 2819
SapI GCTCTTC 3 cut(s) 411, 454, 1197
SatI GCNGC 7 cut(s) 26, 29, 198, 714, 751, 2020, 2938
Sau96I GGNCC 4 cut(s) 1630, 2836, 3049, 3165
ScaI AGTACT 1 cut(s) 1398
SchI GAGTC 6 cut(s) 233, 524, 1676, 2189, 3078, 3548
ScrFI CCNGG 6 cut(s) 128, 987, 1012, 1554, 2047, 3549
SduI GDGCHC 2 cut(s) 466, 3487
SexAI ACCWGGT 1 cut(s) 2045
SfaNI GCATC 4 cut(s) 1504, 1697, 1823, 1902
SfcI CTRYAG 3 cut(s) 2184, 2989, 3614
SfuI TTCGAA 3 cut(s) 393, 2727, 2787
SinI GGWCC 2 cut(s) 3049, 3165
SmiMI CAYNNNNRTG 3 cut(s) 271, 626, 2819
SmlI CTYRAG 4 cut(s) 76, 1088, 1545, 2132
SmoI CTYRAG 4 cut(s) 76, 1088, 1545, 2132
SphI GCATGC 2 cut(s) 1109, 3069
SsiI CCGC 6 cut(s) 271, 970, 1812, 2011, 2937, 3288
SspI AATATT 2 cut(s) 1594, 1925
SspMI CTAG 4 cut(s) 1457, 1994, 2340, 2505
StyD4I CCNGG 6 cut(s) 126, 985, 1010, 1552, 2045, 3547
StyI CCWWGG 4 cut(s) 252, 742, 3150, 3383
TaaI ACNGT 7 cut(s) 588, 1172, 1396, 1624, 2588, 2799, 3421
TaiI ACGT 7 cut(s) 310, 582, 1747, 2396, 2811, 3402, 3417
TatI WGTACW 4 cut(s) 59, 1396, 2687, 3106
TauI GCSGC 1 cut(s) 2940
TscAI CASTG 5 cut(s) 1167, 1175, 2863, 3124, 3426
TseFI GTSAC 1 cut(s) 3415
TseI GCWGC 6 cut(s) 25, 28, 197, 713, 750, 2019
Tsp45I GTSAC 1 cut(s) 3415
TspGWI ACGGA 3 cut(s) 351, 657, 2333
TspRI CASTG 5 cut(s) 1167, 1175, 2863, 3124, 3426
Tth111I GACNNNGTC 2 cut(s) 1746, 3421
Van91I CCANNNNNTGG 2 cut(s) 1685, 3527
VpaK11BI GGWCC 2 cut(s) 3049, 3165
VspI ATTAAT 1 cut(s) 2141
XagI CCTNNNNNAGG 1 cut(s) 1131
XapI RAATTY 8 cut(s) 775, 1266, 1733, 2354, 2710, 2743, 2772, 2970
XceI RCATGY 9 cut(s) 631, 893, 1109, 2033, 2116, 2599, 3069, 3113, 3214
XcmI CCANNNNNNNNNTGG 1 cut(s) 1981
XmiI GTMKAC 4 cut(s) 409, 2328, 2449, 3075
XmnI GAANNNNTTC 5 cut(s) 1735, 1914, 2354, 3126, 3572
XspI CTAG 4 cut(s) 1457, 1994, 2340, 2505
ZraI GACGTC 1 cut(s) 1745
ZrmI AGTACT 1 cut(s) 1398
Zsp2I ATGCAT 3 cut(s) 1111, 1497, 3067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.