MD15G1375200.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000654
Physical Location & Seq
Forward (+)
45899194 .. 45902337
3144 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1375200.v1.1.491

Sequence Viewer

Length: 2532 bp
ATGCGAGACTGGTTCCATCCTGGATGTAAAATTATAATAACAACTAGGCATGAACATTTGTTAAAGGCTCATGAAGTTGTAAGGTTTAAGGTTGAAGGATTGCATGAGGATGAATCACTTGAGCTTTTCAGTTGGCATGCCTTTCGACAACCCCATCCTCAAGAAGGTTATATGGAGCTTTCAAGACCCGTGGTTGAACATTGTGGAGGGGTTCCATTAGCTCTTCAAGTTCTGGGATCTTCTCTATATGGAAAAACAATTGATGTATGGAAAAATGCACTACACAACTTAGACGTGATTTCTGAGGGGAAAATTCAAAAGATTCTTGCCATAAGCTTTGATTCTTTACAAGATCATGATAAACGTTTATTCCTGCATATAGCCTGTTTCTTCGTGGGAAAGGACAAGGATTTTACAACTACAATCCTTGACGAATGTGATTTTTCTACAGAGGTTGGACTTCAACATCTGGTTGATAGATGTCTTGTGGAAATTAATGTCCACAACAAGTTAACCATGCATCAATTACTTCAAGACATGGGAAGGGCAATAATTCGTGAAGAATCACCTGAGGACCCTGGAAGACGCACTAGACTGTGGCATAAAGATGCATTTAACGTTTTGAGAAAATTAGCGGGTACGAGAACTATCAAGGGCCTCGTGCTTAACTTTCCCTCAACAGAGTCATCTCCAGTATTAAATGAGGTAGGTTTCGAAACAAAGGCATTTACAGAGATGCTCAATCTTGAACTACTTCTGCTTGATAATGTAAAGTTGAGTGGAAGCTATGAAGATTTTCCAAAAAATTTAATATGGCTATCTTGGCGAGGATTCTCTTTAAAATCAATACCAGCCAATTTTTGTTTGGAAAATCTAGTTGTTCTTGACTTACGGAAGAGCAGTCTGCAACATGTTTGGAAAGGAACCAGGTTTCTTACAAGATTGAAAATTCTTAATCTCAGTCATTCGCATGGCCTTAGGACAACATCTGACTTGTCCGGACTCCCTAACCTTGAGAAACTAATTCTTAAGGATTGCATTAATTTGATTGATGTTGATGAATCCATTGGAAACTTGGGGAAACTTATTTTCTTGAATCTAAAAGACTGCAAAAGTCTTATGAAGCTTCCAAAAAGAATGAACATGTTGAGGTCTCTTGAGGAACTTATTCTTACTGGTTGCCCAAAGCTTGTTCTTCATGACAGTGCCACAGTTATTCATTTACACTCTACATCTAGGGATATGAATAAACTTAGACTGTTATCAATTATATCATGGACACCAATCAGGTATTGGTGGATGTGGGCATGGCCGGGAAAGACTCTTCAATCAATGAGTTTCTCTCTGGCAAGTTTACCCCGTTTTTTGGGAAGCTTAAGTTTGTCTTACTGCAACATGTCAGAGGTTCCCAACGATCTGTGTACACTATCTTCGTTGAAGCATTTGAATCTAATTGGTAACCCAATTTTGTGCTTACCACAAAAAATGAAGAGTCTTATTATGCTCGAGACTCTTTTGTTAGATAATTGCACAAACCTCGAAATTCTTCCAGAGCTCCCACCAAGGTTGAAGAGGTTAGAAGCAAAATATTGTACATCATTGAAAAGATTAACAAATTTACCAAACTTATTCAGATCATTGCAATCACTTTTTTGGGGTTGTGAGCATTTAGTTGAAGTTGAAAGCTTGTTGAATAAAAAACCGCTGAGAAGCGCTGACATAGAAATGACAAGATATATGGACCTGTTCAATTTGAAATCCATATCAAGCACTGATGTCGAAATGATCAACTACTTGACCGGTACAACCAGGAAGGCTCCTGTCCAGGTACTCTATGAATGTGGCATATATAGCATTTTCTTTCATGGAAGCAAGATTCCAGATGGATTTAGCTTCACAACAAGATACAGATCTGTGCTGTCAGTTATTGCACCTTCACATCCTAATCCCAAAATCCGAGGCTTGAATTCATGTGTTTTATATGCAAGAGAACCAGGCTTGGTTATTGATGACTATCTTGTTAGTGCTATGCATTTGATTAAGGTCAGTAATCAGACCAAGGGTCTTATGTGGACCTATTCTCCAGTCACAGTAGGTTTTCCAACTGAGACGGAAGATATGCTATGGCTAAGCCATTTTCGATTTGTAAACAATGAATTGGAGTATGGGGACGAAATACGTGTTTCGGTGGAGATGCATGATTTTCGGATAAAGGAGTTCGGAATCCAGCTTGTGTACGAGCAGGAAATGTCTCCTAGTAGCCAGAATAATGTTGTTGCTGGAGATGTGTCAATGTCAACATCAGAGTACCAAATGTGGACAGGAAAATACTTTCTCAGTAATCATAGGCACCGTACTCATCAGGCTCAATTCCGAATGAGACAGGAGAATCCAGCACATAATGAATTTTCATTTGGGCCAGAGCATCAGGTTCATAACTTATTCAAGGCCCAAGGACCATTGGAAAATATTGTGAGAGGATGTCCAGCAGATTCCCCATCTGCTTCTCGTCACCTGTTTTGGCGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

844

Amino Acids

96.81

Weight (kDa)

8.17

Isoelectric Point (pI)

46.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 119 - 189 2.5e-18 Disease resistance protein Roq1-like, winged-helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 35
AccB1I GGYRCC 1 cut(s) 2349
AccIII TCCGGA 1 cut(s) 998
AciI CCGC 2 cut(s) 635, 1703
AclI AACGTT 2 cut(s) 364, 618
AclWI GGATC 1 cut(s) 244
AcoI YGGCCR 1 cut(s) 1310
AcsI RAATTY 7 cut(s) 312, 805, 948, 1542, 1615, 1966, 2405
AfaI GTAC 8 cut(s) 640, 1423, 1594, 1804, 1830, 2237, 2309, 2356
AfeI AGCGCT 1 cut(s) 1714
AfiI CCNNNNNNNGG 2 cut(s) 164, 1366
AflII CTTAAG 2 cut(s) 1028, 1375
AflIII ACRYGT 4 cut(s) 910, 1143, 1395, 2179
AgeI ACCGGT 1 cut(s) 1799
AhdI GACNNNNNGTC 1 cut(s) 2061
AjiI CACGTC 1 cut(s) 295
AjnI CCWGG 6 cut(s) 19, 577, 926, 1808, 1824, 1993
AjuI GAANNNNNNNTTGG 2 cut(s) 2397, 2429
Alw21I GWGCWC 1 cut(s) 1557
Alw26I GTCTC 5 cut(s) 1158, 1502, 2102, 2256, 2374
AlwI GGATC 1 cut(s) 244
Ama87I CYCGRG 1 cut(s) 1505
Aor13HI TCCGGA 1 cut(s) 998
Aor51HI AGCGCT 1 cut(s) 1714
AoxI GGCC 5 cut(s) 655, 973, 1310, 2417, 2448
ApoI RAATTY 7 cut(s) 312, 805, 948, 1542, 1615, 1966, 2405
AseI ATTAAT 2 cut(s) 495, 1041
AsiGI ACCGGT 1 cut(s) 1799
Asp700I GAANNNNTTC 4 cut(s) 753, 795, 1167, 1545
AspLEI GCGC 1 cut(s) 1715
AspS9I GGNCC 7 cut(s) 574, 655, 1741, 2073, 2417, 2449, 2456
AsuC2I CCSGG 1 cut(s) 1314
AsuHPI GGTGA 2 cut(s) 558, 2504
AsuII TTCGAA 1 cut(s) 714
AvaI CYCGRG 1 cut(s) 1505
AvaII GGWCC 4 cut(s) 574, 1741, 2073, 2456
AxyI CCTNAGG 2 cut(s) 570, 977
BanI GGYRCC 1 cut(s) 2349
BanII GRGCYC 1 cut(s) 1557
BarI GAAGNNNNNNTAC 2 cut(s) 1414, 1446
BauI CACGAG 1 cut(s) 659
BbsI GAAGAC 1 cut(s) 589
Bbv12I GWGCWC 1 cut(s) 1557
BccI CCATC 4 cut(s) 24, 162, 1877, 2506
BcgI CGANNNNNNTGC 8 cut(s) 125, 159, 1519, 1553, 1759, 1793, 2186, 2220
BciT130I CCWGG 6 cut(s) 21, 579, 928, 1810, 1826, 1995
BclI TGATCA 1 cut(s) 1785
BcnI CCSGG 1 cut(s) 1314
BcoDI GTCTC 5 cut(s) 1158, 1502, 2102, 2256, 2374
BfaI CTAG 5 cut(s) 45, 591, 875, 1236, 2256
BfmI CTRYAG 1 cut(s) 447
BfoI RGCGCY 1 cut(s) 1716
BfrI CTTAAG 2 cut(s) 1028, 1375
BglII AGATCT 1 cut(s) 1910
BlpI GCTNAGC 1 cut(s) 2129
Bme1390I CCNGG 7 cut(s) 21, 579, 928, 1314, 1810, 1826, 1995
Bme18I GGWCC 4 cut(s) 574, 1741, 2073, 2456
BmeRI GACNNNNNGTC 1 cut(s) 2061
BmeT110I CYCGRG 1 cut(s) 1505
BmgBI CACGTC 1 cut(s) 295
BmgT120I GGNCC 7 cut(s) 574, 655, 1741, 2073, 2417, 2449, 2456
BmiI GGNNCC 7 cut(s) 14, 213, 576, 925, 1407, 1818, 2351
BmrFI CCNGG 7 cut(s) 21, 579, 928, 1314, 1810, 1826, 1995
BmsI GCATC 5 cut(s) 529, 598, 726, 2184, 2434
BpiI GAAGAC 1 cut(s) 589
BplI GAGNNNNNCTC 2 cut(s) 1327, 1359
BpmI CTGGAG 3 cut(s) 675, 2067, 2301
Bpu1102I GCTNAGC 1 cut(s) 2129
Bpu14I TTCGAA 1 cut(s) 714
BpuEI CTTGAG 4 cut(s) 140, 144, 1034, 1178
BpuMI CCSGG 1 cut(s) 1314
BsaAI YACGTR 1 cut(s) 2180
BsaBI GATNNNNATC 2 cut(s) 1909, 2013
BsaI GGTCTC 1 cut(s) 1158
BsaJI CCNNGG 6 cut(s) 189, 577, 1562, 1957, 2058, 2452
BsaWI WCCGGW 2 cut(s) 998, 1799
BsaXI ACNNNNNCTCC 2 cut(s) 2065, 2095
Bsc4I CCNNNNNNNGG 2 cut(s) 164, 1366
Bse118I RCCGGY 1 cut(s) 1799
Bse1I ACTGG 4 cut(s) 14, 692, 1180, 2084
Bse21I CCTNAGG 2 cut(s) 570, 977
Bse3DI GCAATG 1 cut(s) 1637
Bse8I GATNNNNATC 2 cut(s) 1909, 2013
BseAI TCCGGA 1 cut(s) 998
BseBI CCWGG 6 cut(s) 21, 579, 928, 1810, 1826, 1995
BseDI CCNNGG 6 cut(s) 189, 577, 1562, 1957, 2058, 2452
BseGI GGATG 7 cut(s) 16, 29, 115, 154, 1305, 1939, 2486
BseJI GATNNNNATC 2 cut(s) 1909, 2013
BseLI CCNNNNNNNGG 2 cut(s) 164, 1366
BseMI GCAATG 1 cut(s) 1637
BseMII CTCAG 6 cut(s) 294, 561, 973, 1697, 2097, 2350
BseNI ACTGG 4 cut(s) 14, 692, 1180, 2084
BshFI GGCC 5 cut(s) 657, 975, 1312, 2419, 2450
BshNI GGYRCC 1 cut(s) 2349
BshTI ACCGGT 1 cut(s) 1799
BsiHKAI GWGCWC 1 cut(s) 1557
BsiHKCI CYCGRG 1 cut(s) 1505
BsiSI CCGG 3 cut(s) 999, 1313, 1800
BslFI GGGAC 1 cut(s) 2183
BslI CCNNNNNNNGG 2 cut(s) 164, 1366
BsmAI GTCTC 5 cut(s) 1158, 1502, 2102, 2256, 2374
BsmBI CGTCTC 1 cut(s) 2102
BsmFI GGGAC 1 cut(s) 2183
BsnI GGCC 5 cut(s) 657, 975, 1312, 2419, 2450
Bso31I GGTCTC 1 cut(s) 1158
BsoBI CYCGRG 1 cut(s) 1505
Bsp119I TTCGAA 1 cut(s) 714
Bsp1286I GDGCHC 1 cut(s) 1557
Bsp13I TCCGGA 1 cut(s) 998
Bsp1407I TGTACA 2 cut(s) 1421, 1592
Bsp143I GATC 6 cut(s) 236, 352, 1414, 1634, 1785, 1910
Bsp1720I GCTNAGC 1 cut(s) 2129
BspACI CCGC 2 cut(s) 635, 1703
BspANI GGCC 5 cut(s) 657, 975, 1312, 2419, 2450
BspCNI CTCAG 6 cut(s) 295, 562, 972, 1698, 2098, 2349
BspEI TCCGGA 1 cut(s) 998
BspHI TCATGA 3 cut(s) 70, 355, 1198
BspLI GGNNCC 7 cut(s) 14, 213, 576, 925, 1407, 1818, 2351
BspPI GGATC 1 cut(s) 244
BspQI GCTCTTC 2 cut(s) 228, 890
BspT104I TTCGAA 1 cut(s) 714
BspT107I GGYRCC 1 cut(s) 2349
BspTI CTTAAG 2 cut(s) 1028, 1375
BspTNI GGTCTC 1 cut(s) 1158
BsrDI GCAATG 1 cut(s) 1637
BsrFI RCCGGY 1 cut(s) 1799
BsrGI TGTACA 2 cut(s) 1421, 1592
BsrI ACTGG 4 cut(s) 14, 692, 1180, 2084
BssAI RCCGGY 1 cut(s) 1799
BssECI CCNNGG 6 cut(s) 189, 577, 1562, 1957, 2058, 2452
BssMI GATC 6 cut(s) 236, 352, 1414, 1634, 1785, 1910
BssSI CACGAG 1 cut(s) 659
BssT1I CCWWGG 3 cut(s) 1562, 2058, 2452
Bst2BI CACGAG 1 cut(s) 659
Bst2UI CCWGG 6 cut(s) 21, 579, 928, 1810, 1826, 1995
Bst4CI ACNGT 6 cut(s) 597, 1205, 1213, 1260, 2092, 2354
Bst6I CTCTTC 5 cut(s) 228, 890, 1329, 1484, 1565
BstAFI CTTAAG 2 cut(s) 1028, 1375
BstAUI TGTACA 2 cut(s) 1421, 1592
BstBAI YACGTR 1 cut(s) 2180
BstBI TTCGAA 1 cut(s) 714
BstC8I GCNNGC 1 cut(s) 138
BstDSI CCRYGG 1 cut(s) 189
BstEII GGTNACC 1 cut(s) 1457
BstENI CCTNNNNNAGG 1 cut(s) 162
BstF5I GGATG 7 cut(s) 16, 29, 115, 154, 1305, 1939, 2486
BstH2I RGCGCY 1 cut(s) 1716
BstHHI GCGC 1 cut(s) 1715
BstKTI GATC 6 cut(s) 239, 355, 1417, 1637, 1788, 1913
BstMAI GTCTC 5 cut(s) 1158, 1502, 2102, 2256, 2374
BstMBI GATC 6 cut(s) 236, 352, 1414, 1634, 1785, 1910
BstMWI GCNNNNNNNGC 2 cut(s) 823, 1851
BstNI CCWGG 6 cut(s) 21, 579, 928, 1810, 1826, 1995
BstNSI RCATGY 4 cut(s) 140, 914, 1147, 1399
BstPI GGTNACC 1 cut(s) 1457
BstSCI CCNGG 7 cut(s) 19, 577, 926, 1312, 1808, 1824, 1993
BstSFI CTRYAG 1 cut(s) 447
BstV2I GAAGAC 1 cut(s) 589
BstX2I RGATCY 2 cut(s) 236, 1910
BstYI RGATCY 2 cut(s) 236, 1910
Bsu36I CCTNAGG 2 cut(s) 570, 977
BsuRI GGCC 5 cut(s) 657, 975, 1312, 2419, 2450
BtgI CCRYGG 1 cut(s) 189
BtrI CACGTC 1 cut(s) 295
BtsCI GGATG 7 cut(s) 16, 29, 115, 154, 1305, 1939, 2486
BtsIMutI CAGTG 2 cut(s) 1210, 1770
Cac8I GCNNGC 1 cut(s) 138
CciI TCATGA 3 cut(s) 70, 355, 1198
CfoI GCGC 1 cut(s) 1715
Cfr10I RCCGGY 1 cut(s) 1799
Cfr13I GGNCC 7 cut(s) 574, 655, 1741, 2073, 2417, 2449, 2456
CseI GACGC 1 cut(s) 594
CsiI ACCWGGT 1 cut(s) 926
Csp6I GTAC 8 cut(s) 639, 1422, 1593, 1803, 1829, 2236, 2308, 2355
CspAI ACCGGT 1 cut(s) 1799
CspCI CAANNNNNGTGG 4 cut(s) 171, 206, 1548, 1583
CviQI GTAC 8 cut(s) 639, 1422, 1593, 1803, 1829, 2236, 2308, 2355
DpnI GATC 6 cut(s) 238, 354, 1416, 1636, 1787, 1912
DpnII GATC 6 cut(s) 236, 352, 1414, 1634, 1785, 1910
DraI TTTAAA 1 cut(s) 840
DriI GACNNNNNGTC 1 cut(s) 2061
EaeI YGGCCR 1 cut(s) 1310
Eam1104I CTCTTC 5 cut(s) 228, 890, 1329, 1484, 1565
Eam1105I GACNNNNNGTC 1 cut(s) 2061
EarI CTCTTC 5 cut(s) 228, 890, 1329, 1484, 1565
Ecl136II GAGCTC 1 cut(s) 1555
Eco130I CCWWGG 3 cut(s) 1562, 2058, 2452
Eco24I GRGCYC 1 cut(s) 1557
Eco31I GGTCTC 1 cut(s) 1158
Eco47I GGWCC 4 cut(s) 574, 1741, 2073, 2456
Eco47III AGCGCT 1 cut(s) 1714
Eco53kI GAGCTC 1 cut(s) 1555
Eco81I CCTNAGG 2 cut(s) 570, 977
Eco88I CYCGRG 1 cut(s) 1505
Eco91I GGTNACC 1 cut(s) 1457
EcoICRI GAGCTC 1 cut(s) 1555
EcoNI CCTNNNNNAGG 1 cut(s) 162
EcoO109I RGGNCCY 2 cut(s) 574, 655
EcoO65I GGTNACC 1 cut(s) 1457
EcoRI GAATTC 1 cut(s) 1966
EcoRII CCWGG 6 cut(s) 19, 577, 926, 1808, 1824, 1993
EcoT14I CCWWGG 3 cut(s) 1562, 2058, 2452
EcoT22I ATGCAT 4 cut(s) 522, 613, 2034, 2199
EcoT38I GRGCYC 1 cut(s) 1557
ErhI CCWWGG 3 cut(s) 1562, 2058, 2452
Esp3I CGTCTC 1 cut(s) 2102
FalI AAGNNNNNCTT 2 cut(s) 1369, 1401
FaqI GGGAC 1 cut(s) 2183
FauI CCCGC 1 cut(s) 628
FbaI TGATCA 1 cut(s) 1785
FokI GGATG 7 cut(s) 3, 36, 122, 141, 1312, 1926, 2493
FriOI GRGCYC 1 cut(s) 1557
FspBI CTAG 5 cut(s) 45, 591, 875, 1236, 2256
GlaI GCGC 1 cut(s) 1714
GsuI CTGGAG 3 cut(s) 675, 2067, 2301
HaeII RGCGCY 1 cut(s) 1716
HaeIII GGCC 5 cut(s) 657, 975, 1312, 2419, 2450
HapII CCGG 3 cut(s) 999, 1313, 1800
HgaI GACGC 1 cut(s) 594
HhaI GCGC 1 cut(s) 1715
Hin6I GCGC 1 cut(s) 1713
HinP1I GCGC 1 cut(s) 1713
HincII GTYRAC 2 cut(s) 513, 2298
HindII GTYRAC 2 cut(s) 513, 2298
HindIII AAGCTT 5 cut(s) 334, 1124, 1187, 1372, 1684
HpaI GTTAAC 1 cut(s) 513
HpaII CCGG 3 cut(s) 999, 1313, 1800
HphI GGTGA 2 cut(s) 558, 2504
HpyAV CCTTC 5 cut(s) 89, 158, 537, 1807, 1944
HpyCH4III ACNGT 6 cut(s) 597, 1205, 1213, 1260, 2092, 2354
HpyCH4IV ACGT 4 cut(s) 294, 364, 618, 2179
HpyF10VI GCNNNNNNNGC 2 cut(s) 823, 1851
HpySE526I ACGT 4 cut(s) 294, 364, 618, 2179
HspAI GCGC 1 cut(s) 1713
Kpn2I TCCGGA 1 cut(s) 998
Ksp22I TGATCA 1 cut(s) 1785
KspAI GTTAAC 1 cut(s) 513
Kzo9I GATC 6 cut(s) 236, 352, 1414, 1634, 1785, 1910
LguI GCTCTTC 2 cut(s) 228, 890
LmnI GCTCC 3 cut(s) 175, 1560, 1822
LweI GCATC 5 cut(s) 529, 598, 726, 2184, 2434
MabI ACCWGGT 1 cut(s) 926
MaeI CTAG 5 cut(s) 45, 591, 875, 1236, 2256
MaeII ACGT 4 cut(s) 294, 364, 618, 2179
MaeIII GTNAC 3 cut(s) 1457, 2086, 2510
MalI GATC 6 cut(s) 238, 354, 1416, 1636, 1787, 1912
MboI GATC 6 cut(s) 236, 352, 1414, 1634, 1785, 1910
MfeI CAATTG 1 cut(s) 258
MflI RGATCY 2 cut(s) 236, 1910
MhlI GDGCHC 1 cut(s) 1557
MlyI GAGTC 5 cut(s) 692, 996, 1315, 1501, 1504
MmeI TCCRAC 2 cut(s) 436, 2126
Mph1103I ATGCAT 4 cut(s) 522, 613, 2034, 2199
MroI TCCGGA 1 cut(s) 998
MroXI GAANNNNTTC 4 cut(s) 753, 795, 1167, 1545
MslI CAYNNNNRTG 5 cut(s) 108, 606, 969, 1203, 1724
MspA1I CMGCKG 1 cut(s) 1705
MspCI CTTAAG 2 cut(s) 1028, 1375
MspI CCGG 3 cut(s) 999, 1313, 1800
MspR9I CCNGG 7 cut(s) 21, 579, 928, 1314, 1810, 1826, 1995
MunI CAATTG 1 cut(s) 258
MvaI CCWGG 6 cut(s) 21, 579, 928, 1810, 1826, 1995
MwoI GCNNNNNNNGC 2 cut(s) 823, 1851
NciI CCSGG 1 cut(s) 1314
NdeII GATC 6 cut(s) 236, 352, 1414, 1634, 1785, 1910
NlaIV GGNNCC 7 cut(s) 14, 213, 576, 925, 1407, 1818, 2351
NmuCI GTSAC 2 cut(s) 2086, 2510
NsiI ATGCAT 4 cut(s) 522, 613, 2034, 2199
NspI RCATGY 4 cut(s) 140, 914, 1147, 1399
NspV TTCGAA 1 cut(s) 714
PaeI GCATGC 1 cut(s) 140
PaeR7I CTCGAG 1 cut(s) 1505
PagI TCATGA 3 cut(s) 70, 355, 1198
PciI ACATGT 3 cut(s) 910, 1143, 1395
PciSI GCTCTTC 2 cut(s) 228, 890
PdmI GAANNNNTTC 4 cut(s) 753, 795, 1167, 1545
PfoI TCCNGGA 1 cut(s) 19
PinAI ACCGGT 1 cut(s) 1799
PleI GAGTC 5 cut(s) 691, 996, 1315, 1500, 1504
PpsI GAGTC 5 cut(s) 691, 996, 1315, 1500, 1504
Ppu21I YACGTR 1 cut(s) 2180
PpuMI RGGWCCY 1 cut(s) 574
PscI ACATGT 3 cut(s) 910, 1143, 1395
PshBI ATTAAT 2 cut(s) 495, 1041
PsiI TTATAA 1 cut(s) 35
Psp124BI GAGCTC 1 cut(s) 1557
Psp1406I AACGTT 2 cut(s) 364, 618
Psp5II RGGWCCY 1 cut(s) 574
Psp6I CCWGG 6 cut(s) 19, 577, 926, 1808, 1824, 1993
PspEI GGTNACC 1 cut(s) 1457
PspGI CCWGG 6 cut(s) 19, 577, 926, 1808, 1824, 1993
PspN4I GGNNCC 7 cut(s) 14, 213, 576, 925, 1407, 1818, 2351
PspPI GGNCC 7 cut(s) 574, 655, 1741, 2073, 2417, 2449, 2456
PspPPI RGGWCCY 1 cut(s) 574
PsuI RGATCY 2 cut(s) 236, 1910
RsaI GTAC 8 cut(s) 640, 1423, 1594, 1804, 1830, 2237, 2309, 2356
RsaNI GTAC 8 cut(s) 639, 1422, 1593, 1803, 1829, 2236, 2308, 2355
RseI CAYNNNNRTG 5 cut(s) 108, 606, 969, 1203, 1724
SacI GAGCTC 1 cut(s) 1557
SapI GCTCTTC 2 cut(s) 228, 890
Sau3AI GATC 6 cut(s) 236, 352, 1414, 1634, 1785, 1910
Sau96I GGNCC 7 cut(s) 574, 655, 1741, 2073, 2417, 2449, 2456
SchI GAGTC 5 cut(s) 692, 996, 1315, 1501, 1504
ScrFI CCNGG 7 cut(s) 21, 579, 928, 1314, 1810, 1826, 1995
SduI GDGCHC 1 cut(s) 1557
SexAI ACCWGGT 1 cut(s) 926
SfaNI GCATC 5 cut(s) 529, 598, 726, 2184, 2434
SfcI CTRYAG 1 cut(s) 447
Sfr274I CTCGAG 1 cut(s) 1505
SfuI TTCGAA 1 cut(s) 714
SinI GGWCC 4 cut(s) 574, 1741, 2073, 2456
SlaI CTCGAG 1 cut(s) 1505
SmiMI CAYNNNNRTG 5 cut(s) 108, 606, 969, 1203, 1724
SmlI CTYRAG 7 cut(s) 119, 159, 1013, 1028, 1157, 1375, 1505
SmoI CTYRAG 7 cut(s) 119, 159, 1013, 1028, 1157, 1375, 1505
SphI GCATGC 1 cut(s) 140
SsiI CCGC 2 cut(s) 635, 1703
SspI AATATT 2 cut(s) 1589, 2470
SspMI CTAG 5 cut(s) 45, 591, 875, 1236, 2256
SstI GAGCTC 1 cut(s) 1557
StyD4I CCNGG 7 cut(s) 19, 577, 926, 1312, 1808, 1824, 1993
StyI CCWWGG 3 cut(s) 1562, 2058, 2452
TaaI ACNGT 6 cut(s) 597, 1205, 1213, 1260, 2092, 2354
TaiI ACGT 4 cut(s) 297, 367, 621, 2182
TaqI TCGA 6 cut(s) 145, 714, 1506, 1539, 1779, 2140
TatI WGTACW 2 cut(s) 1421, 1592
TscAI CASTG 2 cut(s) 1210, 1777
TseFI GTSAC 2 cut(s) 2086, 2510
Tsp45I GTSAC 2 cut(s) 2086, 2510
TspGWI ACGGA 2 cut(s) 907, 2126
TspRI CASTG 2 cut(s) 1210, 1777
Vha464I CTTAAG 2 cut(s) 1028, 1375
VpaK11BI GGWCC 4 cut(s) 574, 1741, 2073, 2456
VspI ATTAAT 2 cut(s) 495, 1041
XagI CCTNNNNNAGG 1 cut(s) 162
XapI RAATTY 7 cut(s) 312, 805, 948, 1542, 1615, 1966, 2405
XceI RCATGY 4 cut(s) 140, 914, 1147, 1399
XcmI CCANNNNNNNNNTGG 3 cut(s) 862, 1072, 1290
XhoI CTCGAG 1 cut(s) 1505
XmnI GAANNNNTTC 4 cut(s) 753, 795, 1167, 1545
XspI CTAG 5 cut(s) 45, 591, 875, 1236, 2256
Zsp2I ATGCAT 4 cut(s) 522, 613, 2034, 2199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.