Rh4AG131500

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000654
Physical Location & Seq
Forward (+)
28568962 .. 28573728
4767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG131500.1

Sequence Viewer

Length: 3603 bp
ATGAGCACACAGTTGGATGCTTCTTCTTCTTCTTCTTCTTCCTCCTGCTCCCCTTACCGGTGTACTTATCATGCATTCTTGAGTTTCAGAGGTGAGGACACACGTAAGGGATTTACCGATCATCTTTACAGGGCCTTGGAGCTCGCAGGAATCCACACGTTTAGAGACGATGATGAAATCGAGAGAGGAGCAAATATCGCAGCAGAGTTACAGAGAGCTATCCAAGAGTCGAGGGTATCTGTGGTTGTGTTGTCTAGAGACTATGCTTCATCAAGGTGGTGCTTGGACGAACTTGCAAAGATCATGGAACGTAGGAGAAATGATGACGCACACGTGGTTATTCCAGTGTTTTATGATGTAGATCCATCCCATGTCAGGAAGCAAATCGGGAGTTTTGCTGAAGCTTTTGTTAGACATGAAGAGCGCTTCAAGGAGGATATGGGAAAGGTTGCCGAGTGGAGAAGAGCGCTTTCTGATGTTGCAGATCTTGGTGGAATGGTTTTAGGACAACGGTATGAATCACAATTTATCCAAGATATTGTTGAAGAAATCAGAAGTAAATTGGATTCCAAAGTCTTAAACATTGCTCCGTATGCAATTGGAATAGATGATCGTGTTCGAGGTATAAACATGTGGTTAGAAGATGGGTCAAATGATGTTGGTGTGGCTGTGATCTATGGAATGGGTGGAATTGGCAAAACCACTATTGCCAAAGCTGCTTATAACTTGAATTTCCATAGATTTCATGGTAGCAGCTTTCTTGCTGATGTTAGAGAAACTTCAGAACAGCTGAATGGTTTGGTTCGCTTACAAAGAAATCTTCTTTCAGATCTCCAGAAGGGGAAAACAAAGAAAATATACAACATTGATGAAGGAATACGAAAGATAAAAGATGCTGTATGTTGCAAGAAAGTTCTTATTGTTCTTGATGATGTGAACCACTCAGACCAATTTAATGCAATTATTGGAATGCGCAGATGGTTTGTTCCAGGAAGTAAGATAATCTTAACAACCAGATACGAACATTTGTTAAAGGCTCACGAAGTTAACACAATGTTTAAGGTTCAGGAGTTGAATGAGCATGAATCACTTGAGCTCCTCAGTTGGCACGCCTTTGGACAAGCCCATCCTATTGAAGCTTACATTGATCTTTCAAAACCCGTTGTAAAACACTGTGGAGGGATTCCATTAGCTCTGCAAGTTCTGGGATCTTCTTTGTCTGGTAGAAGTATAGATATATGGCAAAGCGCATTACAGAAGCTATGTGAGATTCCAGATGGAGAAATTCAAAAAATTCTTAGAATCAGTTTTGACTCTCTGCAAGATGATCATGACAAAAACTTATTCCTTCATATAGCATGCTTCTTCAGAGGAAAGAATAAAGAGTTTTCAATTACAGTACTGGACAACCTTAAATTCTACACAGTGATTGGAATTCAAAATCTGGTTGATAGATGTCTAGTGAAAATTAATGAAGACAACAAGTTGATAGTGCATCATTTGCTTCGAGACATGGCAAGGGGAATTATTCGCGAAGAATCACCTGAGGACCCTGGGAAACGCAGTAGGGTGTGGCACAAAGATGCCTTCAATATTTTGAGTAAAAGAACTCAGGGTACTGGAACTGTTAAGGGCATCATGCTTGACCTTTCTATGTTGATGCAAGATGAGTCTTCCAAAACATTATTTAGTGGAAGTAACAGCAAACGACGTCGTGTTGATGATTATGATGGAAATTGTTCAGGACGGCGTCGACTTGGTCTCTTCACCTGGCAATTATTTAGTTTTTCCTCAGCCAACACGACTCCAGCATCAGATGAGGTTGACCTCAAAATAGAGGCATTCAAAAAGATGCACAACCTTGAAGTTCTCCTACTCAATAATGTACATTTCAGTCAAGGCTTTGAAAATTTTCCAAAAAACTTGATATGGCTGTCTTGGCGAGGATTCTCTTTAAATTCGATACCAGCCAGTTTGTATTTGAAAACTCTAGTTGTTCTTGACTTGCGGAATAGCAGCCTGCAATGTGTTTGGAAGGGAACCAAGTCTCTTCCAAGTCTGAAAGTCCTTAATCTTAGTCATTCACATGGCCTTTCAGCAACCCCCGACATATCAGGACTCCCTAACCTTGAGAGATTGATTCTAAAAAATTGTAGAAATTTGGTTGAGGTTGATGAATCGATTGGAGACTTGGAGAAACTTGTTTTCTTAAATCTAAAAGACTGCAAAAATCTGATGAAGCTTCCGACCAGAATCAAAATGTCAAGTTCTCTTCAAACACTTATTCTTTCTGGTTGCTCAAAGCTTGTGCTTCATTCTAATATGGTTGCAACAAATCAGTCACTCTTGACAGCTAGTGATATGAAGAAAGACGATTGTTCATCACCTAACTTGTGGAAATCACTCTTATTATGGGCAGTACCAAGCAGAAATATCATACCAACCAGTTTCTCAGTATCAAATTTACCACGTTCTTTACGAAGCTTAAGCTTAGCTGACTGCAACCTATTAGAGATTCCAGGTGATCTTAGTATCCTCTCCTCATTGAAGTATTTAGACCTATGTGGGAACCCAATTCAGAGCCTCCCAGAAAACTTAAGATCTCTTAGTAAGCTTGAGACACTTATGTTAGAGGACTGCACAGAACTCAGAACGCTTCCAGAGCTCCCACCAAGTCTCCAGAGTTTGCTTGCAAGTTCTTGTAAATCATTGAAAAGAATAACAAATTTACCAAACTTGTTCAAATCATTGGATACAGATTTTCTGCATTGCAAGAAGTTAGTTGAAGTTGAAAGCTTGTTCACTATAAAACCATTGACAACCACTGACATAGAAATGATCAAAGATATGGGACTGTTCAATTTCAAATCCATTGGAAGCTATGCTGAGGTTGAAATGATCAACTACATTACAAATACAACCAAGAAGGCCCCCCTCCAGGTGTTGTCTGAATGTGGCATATCTAGCATTTACGTAAACGGAAATGAGATTCCAGTTTGGTTTCGCCACAGGAGAATGGGCAACTCTGTGTTGTCTATTATAGTACCTCCACATCTTAATCATAGAATTCGAGGCTTAAATGCATGTGTTGTATATGCACGACGCCCAGATATTGGTCGTTCTGCTAAATATCAAAACTTGTTTAAAGTCAGCAATGAGACCAAGGGTCTCATGTGGACCTATTCCCCTATCACAATAGGTATTCCAAAGGAGAATGAAGACATGTTATGGCTAAGCCATTGGCAGTTTGGAGAACATGATCTGGGAGGGGGAGACGAAGTACGTGTTTCGGTTGAGTTTGGACCCGAGCTTGGACACAATCTAAATTTATGGACAAAGGAATTGGGAATCCAACTTGTGTATGAGCAGGAAAGTAGTGAAGATGGAAAGAAACACAATTTCCTTGCTGGAGATGTGTCAGTGTCATCCTCAAGTTACCAAATGTGGACGGGAAAATACTTTCTTTGTAATAACCTGAATCGTGCTCAGAAAACACAATTCAAGAGTAGCCAGGAAAACCCATCTCATCTTGATTCTGACAAAACCAAGAGCCCCTTCCTTTTCTTATTTGAGCATGATGATGTTTCACAGGCTGATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1200

Amino Acids

135.67

Weight (kDa)

7.57

Isoelectric Point (pI)

45.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 23 - 191 9e-55 TIR domain
TIR_2 PF13676 26 - 123 3.8e-18 TIR domain
NB-ARC PF00931 204 - 372 5.4e-20 NB-ARC domain
WHD_ROQ1 PF23282 443 - 514 5.8e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 654 - 765 4e-06 Leucine-rich repeat region
LRR_14 PF23598 824 - 917 1.2e-07 Leucine-rich repeat region
LRR_8 PF13855 824 - 880 2.6e-06 Leucine rich repeat
C-JID PF20160 993 - 1125 9.2e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 723
AatII GACGTC 1 cut(s) 1714
Acc16I TGCGCA 1 cut(s) 974
AccB7I CCANNNNNTGG 1 cut(s) 3113
AccI GTMKAC 1 cut(s) 1753
AccII CGCG 1 cut(s) 1533
AciI CCGC 1 cut(s) 2008
AclWI GGATC 2 cut(s) 356, 1216
AcuI CTGAAG 3 cut(s) 420, 765, 1351
AcvI CACGTG 1 cut(s) 334
AcyI GRCGYC 3 cut(s) 1711, 1750, 3103
AfaI GTAC 7 cut(s) 64, 1401, 1618, 1887, 2421, 3045, 3282
AfeI AGCGCT 2 cut(s) 425, 468
AfiI CCNNNNNNNGG 5 cut(s) 57, 375, 2938, 3113, 3311
AflII CTTAAG 2 cut(s) 2485, 2596
AflIII ACRYGT 6 cut(s) 101, 156, 331, 630, 3222, 3283
AgeI ACCGGT 1 cut(s) 57
AhdI GACNNNNNGTC 1 cut(s) 3165
AjnI CCWGG 6 cut(s) 988, 1552, 1769, 2518, 2937, 3510
AjuI GAANNNNNNNTTGG 2 cut(s) 3539, 3571
Alw21I GWGCWC 5 cut(s) 8, 144, 1098, 2667, 3487
AlwI GGATC 2 cut(s) 356, 1216
Ama87I CYCGRG 1 cut(s) 3305
Aor51HI AGCGCT 2 cut(s) 425, 468
AoxI GGCC 3 cut(s) 132, 2089, 2928
ApeKI GCWGC 4 cut(s) 200, 716, 753, 2016
AseI ATTAAT 1 cut(s) 1470
AsiGI ACCGGT 1 cut(s) 57
Asp700I GAANNNNTTC 2 cut(s) 1738, 1911
AspLEI GCGC 4 cut(s) 426, 469, 975, 1250
AspS9I GGNCC 5 cut(s) 132, 1549, 2929, 3177, 3302
AsuHPI GGTGA 5 cut(s) 104, 1533, 1759, 2376, 2534
AvaI CYCGRG 1 cut(s) 3305
AvaII GGWCC 3 cut(s) 1549, 3177, 3302
AxyI CCTNAGG 1 cut(s) 1545
BanII GRGCYC 4 cut(s) 144, 1098, 2667, 3554
BarI GAAGNNNNNNTAC 2 cut(s) 1857, 1889
BbrPI CACGTG 1 cut(s) 334
BbsI GAAGAC 3 cut(s) 1482, 1665, 3225
Bbv12I GWGCWC 5 cut(s) 8, 144, 1098, 2667, 3487
BbvCI CCTCAGC 2 cut(s) 1792, 2886
BbvI GCAGC 4 cut(s) 212, 703, 765, 2028
BccI CCATC 8 cut(s) 373, 638, 972, 1134, 1271, 1724, 3377, 3529
BceAI ACGGC 1 cut(s) 1763
BciT130I CCWGG 6 cut(s) 990, 1554, 1771, 2520, 2939, 3512
BciVI GTATCC 2 cut(s) 2543, 2746
BclI TGATCA 4 cut(s) 1327, 2838, 2898, 3595
BfaI CTAG 5 cut(s) 255, 1460, 1991, 2355, 2964
BfoI RGCGCY 2 cut(s) 427, 470
BfrI CTTAAG 2 cut(s) 2485, 2596
BfuI GTATCC 2 cut(s) 2543, 2746
BglII AGATCT 3 cut(s) 484, 829, 2600
BisI GCNGC 4 cut(s) 201, 717, 754, 2017
BlpI GCTNAGC 2 cut(s) 2491, 3233
BlsI GCNGC 4 cut(s) 202, 718, 755, 2018
BmcAI AGTACT 1 cut(s) 1401
Bme1390I CCNGG 6 cut(s) 990, 1554, 1771, 2520, 2939, 3512
Bme18I GGWCC 3 cut(s) 1549, 3177, 3302
BmeRI GACNNNNNGTC 1 cut(s) 3165
BmeT110I CYCGRG 1 cut(s) 3305
BmgT120I GGNCC 5 cut(s) 132, 1549, 2929, 3177, 3302
BmiI GGNNCC 5 cut(s) 1551, 2041, 2570, 2931, 3304
BmrFI CCNGG 6 cut(s) 990, 1554, 1771, 2520, 2939, 3512
BmsI GCATC 8 cut(s) 7, 883, 1504, 1573, 1644, 1650, 1820, 1842
BpiI GAAGAC 3 cut(s) 1482, 1665, 3225
BpmI CTGGAG 5 cut(s) 818, 1791, 2663, 2921, 3429
Bpu10I CCTNAGC 2 cut(s) 1792, 2886
Bpu1102I GCTNAGC 2 cut(s) 2491, 3233
BpuEI CTTGAG 5 cut(s) 100, 1112, 2150, 2636, 3415
Bsa29I ATCGAT 1 cut(s) 2180
BsaAI YACGTR 4 cut(s) 104, 334, 2974, 3284
BsaBI GATNNNNATC 1 cut(s) 360
BsaHI GRCGYC 3 cut(s) 1711, 1750, 3103
BsaI GGTCTC 3 cut(s) 1766, 3152, 3173
BsaJI CCNNGG 4 cut(s) 135, 1552, 1553, 3162
BsaWI WCCGGW 1 cut(s) 57
BsaXI ACNNNNNCTCC 6 cut(s) 1080, 1110, 2661, 2691, 3264, 3294
Bsc4I CCNNNNNNNGG 5 cut(s) 57, 375, 2938, 3113, 3311
Bse118I RCCGGY 1 cut(s) 57
Bse1I ACTGG 6 cut(s) 344, 1407, 1624, 1971, 2445, 2993
Bse21I CCTNAGG 1 cut(s) 1545
Bse3DI GCAATG 4 cut(s) 582, 2030, 2767, 3160
Bse8I GATNNNNATC 1 cut(s) 360
BseBI CCWGG 6 cut(s) 990, 1554, 1771, 2520, 2939, 3512
BseCI ATCGAT 1 cut(s) 2180
BseDI CCNNGG 4 cut(s) 135, 1552, 1553, 3162
BseGI GGATG 4 cut(s) 22, 365, 1126, 3425
BseJI GATNNNNATC 1 cut(s) 360
BseLI CCNNNNNNNGG 5 cut(s) 57, 375, 2938, 3113, 3311
BseMI GCAATG 4 cut(s) 582, 2030, 2767, 3160
BseMII CTCAG 9 cut(s) 957, 1114, 1536, 1625, 1806, 2466, 2662, 2877, 3500
BseNI ACTGG 6 cut(s) 344, 1407, 1624, 1971, 2445, 2993
BseRI GAGGAG 3 cut(s) 201, 1088, 2530
BseXI GCAGC 4 cut(s) 212, 703, 765, 2028
BsgI GTGCAG 1 cut(s) 2623
Bsh1236I CGCG 1 cut(s) 1533
BshFI GGCC 3 cut(s) 134, 2091, 2930
BshTI ACCGGT 1 cut(s) 57
BshVI ATCGAT 1 cut(s) 2180
BsiHKAI GWGCWC 5 cut(s) 8, 144, 1098, 2667, 3487
BsiHKCI CYCGRG 1 cut(s) 3305
BsiSI CCGG 1 cut(s) 58
BslFI GGGAC 1 cut(s) 2865
BslI CCNNNNNNNGG 5 cut(s) 57, 375, 2938, 3113, 3311
BsmBI CGTCTC 2 cut(s) 159, 3267
BsmFI GGGAC 1 cut(s) 2865
BsmI GAATGC 3 cut(s) 74, 975, 1841
BsnI GGCC 3 cut(s) 134, 2091, 2930
Bso31I GGTCTC 3 cut(s) 1766, 3152, 3173
BsoBI CYCGRG 1 cut(s) 3305
Bsp1286I GDGCHC 6 cut(s) 8, 144, 1098, 2667, 3487, 3554
Bsp1407I TGTACA 1 cut(s) 1885
Bsp1720I GCTNAGC 2 cut(s) 2491, 3233
Bsp68I TCGCGA 1 cut(s) 1533
BspACI CCGC 1 cut(s) 2008
BspANI GGCC 3 cut(s) 134, 2091, 2930
BspCNI CTCAG 9 cut(s) 956, 1113, 1537, 1624, 1805, 2465, 2661, 2878, 3499
BspDI ATCGAT 1 cut(s) 2180
BspFNI CGCG 1 cut(s) 1533
BspHI TCATGA 1 cut(s) 1330
BspLI GGNNCC 5 cut(s) 1551, 2041, 2570, 2931, 3304
BspPI GGATC 2 cut(s) 356, 1216
BspQI GCTCTTC 2 cut(s) 414, 457
BspTI CTTAAG 2 cut(s) 2485, 2596
BspTNI GGTCTC 3 cut(s) 1766, 3152, 3173
BsrDI GCAATG 4 cut(s) 582, 2030, 2767, 3160
BsrFI RCCGGY 1 cut(s) 57
BsrGI TGTACA 1 cut(s) 1885
BsrI ACTGG 6 cut(s) 344, 1407, 1624, 1971, 2445, 2993
BssAI RCCGGY 1 cut(s) 57
BssECI CCNNGG 4 cut(s) 135, 1552, 1553, 3162
BssNI GRCGYC 3 cut(s) 1711, 1750, 3103
BssT1I CCWWGG 2 cut(s) 135, 3162
Bst2UI CCWGG 6 cut(s) 990, 1554, 1771, 2520, 2939, 3512
Bst4CI ACNGT 7 cut(s) 12, 513, 1175, 1399, 1426, 1627, 2856
Bst6I CTCTTC 5 cut(s) 414, 457, 1769, 2055, 2277
BstACI GRCGYC 3 cut(s) 1711, 1750, 3103
BstAFI CTTAAG 2 cut(s) 2485, 2596
BstAPI GCANNNNNTGC 1 cut(s) 1501
BstAUI TGTACA 1 cut(s) 1885
BstBAI YACGTR 4 cut(s) 104, 334, 2974, 3284
BstC8I GCNNGC 5 cut(s) 144, 1110, 1360, 2021, 2691
BstF5I GGATG 4 cut(s) 22, 365, 1126, 3425
BstFNI CGCG 1 cut(s) 1533
BstH2I RGCGCY 2 cut(s) 427, 470
BstHHI GCGC 4 cut(s) 426, 469, 975, 1250
BstMWI GCNNNNNNNGC 7 cut(s) 197, 593, 716, 1501, 1939, 2662, 2964
BstNI CCWGG 6 cut(s) 990, 1554, 1771, 2520, 2939, 3512
BstNSI RCATGY 4 cut(s) 634, 1362, 3087, 3226
BstSCI CCNGG 6 cut(s) 988, 1552, 1769, 2518, 2937, 3510
BstSNI TACGTA 1 cut(s) 2974
BstUI CGCG 1 cut(s) 1533
BstV1I GCAGC 4 cut(s) 212, 703, 765, 2028
BstV2I GAAGAC 3 cut(s) 1482, 1665, 3225
BstX2I RGATCY 5 cut(s) 361, 484, 829, 1208, 2600
BstYI RGATCY 5 cut(s) 361, 484, 829, 1208, 2600
Bsu15I ATCGAT 1 cut(s) 2180
Bsu36I CCTNAGG 1 cut(s) 1545
BsuI GTATCC 2 cut(s) 2543, 2746
BsuRI GGCC 3 cut(s) 134, 2091, 2930
BsuTUI ATCGAT 1 cut(s) 2180
BtsCI GGATG 4 cut(s) 22, 365, 1126, 3425
BtsIMutI CAGTG 5 cut(s) 351, 1171, 1431, 2823, 3426
BtuMI TCGCGA 1 cut(s) 1533
Cac8I GCNNGC 5 cut(s) 144, 1110, 1360, 2021, 2691
CciI TCATGA 1 cut(s) 1330
CfoI GCGC 4 cut(s) 426, 469, 975, 1250
Cfr10I RCCGGY 1 cut(s) 57
Cfr13I GGNCC 5 cut(s) 132, 1549, 2929, 3177, 3302
ClaI ATCGAT 1 cut(s) 2180
CseI GACGC 3 cut(s) 335, 1739, 3111
Csp6I GTAC 7 cut(s) 63, 1400, 1617, 1886, 2420, 3044, 3281
CspAI ACCGGT 1 cut(s) 57
CviQI GTAC 7 cut(s) 63, 1400, 1617, 1886, 2420, 3044, 3281
DraI TTTAAA 2 cut(s) 1956, 3145
DriI GACNNNNNGTC 1 cut(s) 3165
Eam1104I CTCTTC 5 cut(s) 414, 457, 1769, 2055, 2277
Eam1105I GACNNNNNGTC 1 cut(s) 3165
EarI CTCTTC 5 cut(s) 414, 457, 1769, 2055, 2277
Ecl136II GAGCTC 3 cut(s) 142, 1096, 2665
Eco105I TACGTA 1 cut(s) 2974
Eco130I CCWWGG 2 cut(s) 135, 3162
Eco24I GRGCYC 4 cut(s) 144, 1098, 2667, 3554
Eco31I GGTCTC 3 cut(s) 1766, 3152, 3173
Eco47I GGWCC 3 cut(s) 1549, 3177, 3302
Eco47III AGCGCT 2 cut(s) 425, 468
Eco53kI GAGCTC 3 cut(s) 142, 1096, 2665
Eco57I CTGAAG 3 cut(s) 420, 765, 1351
Eco72I CACGTG 1 cut(s) 334
Eco81I CCTNAGG 1 cut(s) 1545
Eco88I CYCGRG 1 cut(s) 3305
EcoICRI GAGCTC 3 cut(s) 142, 1096, 2665
EcoO109I RGGNCCY 3 cut(s) 132, 1549, 2929
EcoRI GAATTC 2 cut(s) 1434, 3066
EcoRII CCWGG 6 cut(s) 988, 1552, 1769, 2518, 2937, 3510
EcoT14I CCWWGG 2 cut(s) 135, 3162
EcoT22I ATGCAT 2 cut(s) 76, 3085
EcoT38I GRGCYC 4 cut(s) 144, 1098, 2667, 3554
ErhI CCWWGG 2 cut(s) 135, 3162
Esp3I CGTCTC 2 cut(s) 159, 3267
FalI AAGNNNNNCTT 4 cut(s) 989, 1021, 3539, 3571
FaqI GGGAC 1 cut(s) 2865
FbaI TGATCA 4 cut(s) 1327, 2838, 2898, 3595
FblI GTMKAC 1 cut(s) 1753
Fnu4HI GCNGC 4 cut(s) 201, 717, 754, 2017
FokI GGATG 4 cut(s) 29, 352, 1113, 3412
FriOI GRGCYC 4 cut(s) 144, 1098, 2667, 3554
Fsp4HI GCNGC 4 cut(s) 201, 717, 754, 2017
FspBI CTAG 5 cut(s) 255, 1460, 1991, 2355, 2964
FspI TGCGCA 1 cut(s) 974
GlaI GCGC 4 cut(s) 425, 468, 974, 1249
GluI GCNGC 4 cut(s) 201, 717, 754, 2017
GsuI CTGGAG 5 cut(s) 818, 1791, 2663, 2921, 3429
HaeII RGCGCY 2 cut(s) 427, 470
HaeIII GGCC 3 cut(s) 134, 2091, 2930
HapII CCGG 1 cut(s) 58
HgaI GACGC 3 cut(s) 335, 1739, 3111
HhaI GCGC 4 cut(s) 426, 469, 975, 1250
Hin1I GRCGYC 3 cut(s) 1711, 1750, 3103
Hin6I GCGC 4 cut(s) 424, 467, 973, 1248
HinP1I GCGC 4 cut(s) 424, 467, 973, 1248
HincII GTYRAC 3 cut(s) 1048, 1754, 1825
HindII GTYRAC 3 cut(s) 1048, 1754, 1825
HindIII AAGCTT 8 cut(s) 402, 1137, 2240, 2303, 2482, 2488, 2612, 2794
HpaI GTTAAC 1 cut(s) 1048
HpaII CCGG 1 cut(s) 58
HphI GGTGA 5 cut(s) 104, 1533, 1759, 2376, 2534
Hpy166II GTNNAC 9 cut(s) 63, 937, 1048, 1754, 1825, 2802, 2977, 3177, 3447
Hpy8I GTNNAC 9 cut(s) 63, 937, 1048, 1754, 1825, 2802, 2977, 3177, 3447
Hpy99I CGWCG 4 cut(s) 1713, 1716, 1755, 3105
HpyAV CCTTC 7 cut(s) 832, 866, 1358, 1597, 2029, 2920, 3565
HpyCH4III ACNGT 7 cut(s) 12, 513, 1175, 1399, 1426, 1627, 2856
HpyCH4IV ACGT 8 cut(s) 103, 158, 310, 333, 1711, 2470, 2973, 3283
HpyF10VI GCNNNNNNNGC 7 cut(s) 197, 593, 716, 1501, 1939, 2662, 2964
HpySE526I ACGT 8 cut(s) 103, 158, 310, 333, 1711, 2470, 2973, 3283
Hsp92I GRCGYC 3 cut(s) 1711, 1750, 3103
HspAI GCGC 4 cut(s) 424, 467, 973, 1248
Ksp22I TGATCA 4 cut(s) 1327, 2838, 2898, 3595
KspAI GTTAAC 1 cut(s) 1048
LguI GCTCTTC 2 cut(s) 414, 457
LmnI GCTCC 6 cut(s) 53, 139, 188, 592, 1101, 2670
Lsp1109I GCAGC 4 cut(s) 212, 703, 765, 2028
LweI GCATC 8 cut(s) 7, 883, 1504, 1573, 1644, 1650, 1820, 1842
MaeI CTAG 5 cut(s) 255, 1460, 1991, 2355, 2964
MaeII ACGT 8 cut(s) 103, 158, 310, 333, 1711, 2470, 2973, 3283
MaeIII GTNAC 4 cut(s) 207, 1697, 2340, 3434
MfeI CAATTG 1 cut(s) 597
MflI RGATCY 5 cut(s) 361, 484, 829, 1208, 2600
MhlI GDGCHC 6 cut(s) 8, 144, 1098, 2667, 3487, 3554
MlyI GAGTC 5 cut(s) 236, 1307, 1679, 1798, 2112
MmeI TCCRAC 2 cut(s) 2270, 3376
Mph1103I ATGCAT 2 cut(s) 76, 3085
MroXI GAANNNNTTC 2 cut(s) 1738, 1911
MslI CAYNNNNRTG 5 cut(s) 274, 1581, 2085, 2834, 3579
MspA1I CMGCKG 1 cut(s) 790
MspCI CTTAAG 2 cut(s) 2485, 2596
MspI CCGG 1 cut(s) 58
MspR9I CCNGG 6 cut(s) 990, 1554, 1771, 2520, 2939, 3512
MunI CAATTG 1 cut(s) 597
Mva1269I GAATGC 3 cut(s) 74, 975, 1841
MvaI CCWGG 6 cut(s) 990, 1554, 1771, 2520, 2939, 3512
MvnI CGCG 1 cut(s) 1533
MwoI GCNNNNNNNGC 7 cut(s) 197, 593, 716, 1501, 1939, 2662, 2964
NlaIV GGNNCC 5 cut(s) 1551, 2041, 2570, 2931, 3304
NmeAIII GCCGAG 1 cut(s) 478
NmuCI GTSAC 1 cut(s) 2340
NruI TCGCGA 1 cut(s) 1533
NsbI TGCGCA 1 cut(s) 974
NsiI ATGCAT 2 cut(s) 76, 3085
NspI RCATGY 4 cut(s) 634, 1362, 3087, 3226
PaeI GCATGC 1 cut(s) 1362
PagI TCATGA 1 cut(s) 1330
PasI CCCWGGG 1 cut(s) 1553
PciI ACATGT 2 cut(s) 630, 3222
PciSI GCTCTTC 2 cut(s) 414, 457
PcsI WCGNNNNNNNCGW 1 cut(s) 2476
PctI GAATGC 3 cut(s) 74, 975, 1841
PdmI GAANNNNTTC 2 cut(s) 1738, 1911
PflFI GACNNNGTC 2 cut(s) 1749, 1758
PflMI CCANNNNNTGG 1 cut(s) 3113
PfoI TCCNGGA 1 cut(s) 988
PinAI ACCGGT 1 cut(s) 57
PkrI GCNGC 4 cut(s) 202, 718, 755, 2018
PleI GAGTC 5 cut(s) 235, 1307, 1678, 1798, 2112
PmaCI CACGTG 1 cut(s) 334
PmlI CACGTG 1 cut(s) 334
PpsI GAGTC 5 cut(s) 235, 1307, 1678, 1798, 2112
Ppu21I YACGTR 4 cut(s) 104, 334, 2974, 3284
PpuMI RGGWCCY 1 cut(s) 1549
PscI ACATGT 2 cut(s) 630, 3222
PshBI ATTAAT 1 cut(s) 1470
PsiI TTATAA 1 cut(s) 723
Psp124BI GAGCTC 3 cut(s) 144, 1098, 2667
Psp5II RGGWCCY 1 cut(s) 1549
Psp6I CCWGG 6 cut(s) 988, 1552, 1769, 2518, 2937, 3510
PspCI CACGTG 1 cut(s) 334
PspGI CCWGG 6 cut(s) 988, 1552, 1769, 2518, 2937, 3510
PspN4I GGNNCC 5 cut(s) 1551, 2041, 2570, 2931, 3304
PspPI GGNCC 5 cut(s) 132, 1549, 2929, 3177, 3302
PspPPI RGGWCCY 1 cut(s) 1549
PsrI GAACNNNNNNTAC 2 cut(s) 1600, 1632
PsuI RGATCY 5 cut(s) 361, 484, 829, 1208, 2600
PsyI GACNNNGTC 2 cut(s) 1749, 1758
PvuII CAGCTG 1 cut(s) 790
RruI TCGCGA 1 cut(s) 1533
RsaI GTAC 7 cut(s) 64, 1401, 1618, 1887, 2421, 3045, 3282
RsaNI GTAC 7 cut(s) 63, 1400, 1617, 1886, 2420, 3044, 3281
RseI CAYNNNNRTG 5 cut(s) 274, 1581, 2085, 2834, 3579
SacI GAGCTC 3 cut(s) 144, 1098, 2667
SalI GTCGAC 1 cut(s) 1752
SapI GCTCTTC 2 cut(s) 414, 457
SatI GCNGC 4 cut(s) 201, 717, 754, 2017
Sau96I GGNCC 5 cut(s) 132, 1549, 2929, 3177, 3302
ScaI AGTACT 1 cut(s) 1401
SchI GAGTC 5 cut(s) 236, 1307, 1679, 1798, 2112
ScrFI CCNGG 6 cut(s) 990, 1554, 1771, 2520, 2939, 3512
SduI GDGCHC 6 cut(s) 8, 144, 1098, 2667, 3487, 3554
SfaNI GCATC 8 cut(s) 7, 883, 1504, 1573, 1644, 1650, 1820, 1842
SinI GGWCC 3 cut(s) 1549, 3177, 3302
SmiMI CAYNNNNRTG 5 cut(s) 274, 1581, 2085, 2834, 3579
SmlI CTYRAG 7 cut(s) 79, 1091, 2129, 2485, 2596, 2615, 3430
SmoI CTYRAG 7 cut(s) 79, 1091, 2129, 2485, 2596, 2615, 3430
SnaBI TACGTA 1 cut(s) 2974
SphI GCATGC 1 cut(s) 1362
SsiI CCGC 1 cut(s) 2008
SspI AATATT 1 cut(s) 1594
SspMI CTAG 5 cut(s) 255, 1460, 1991, 2355, 2964
SstI GAGCTC 3 cut(s) 144, 1098, 2667
StyD4I CCNGG 6 cut(s) 988, 1552, 1769, 2518, 2937, 3510
StyI CCWWGG 2 cut(s) 135, 3162
TaaI ACNGT 7 cut(s) 12, 513, 1175, 1399, 1426, 1627, 2856
TaiI ACGT 8 cut(s) 106, 161, 313, 336, 1714, 2473, 2976, 3286
TaqI TCGA 8 cut(s) 180, 230, 619, 1507, 1753, 1961, 2180, 3070
TatI WGTACW 3 cut(s) 62, 1399, 1885
TscAI CASTG 5 cut(s) 351, 1178, 1431, 2830, 3426
TseFI GTSAC 1 cut(s) 2340
TseI GCWGC 4 cut(s) 200, 716, 753, 2016
Tsp45I GTSAC 1 cut(s) 2340
TspGWI ACGGA 2 cut(s) 579, 2994
TspRI CASTG 5 cut(s) 351, 1178, 1431, 2830, 3426
Tth111I GACNNNGTC 2 cut(s) 1749, 1758
Van91I CCANNNNNTGG 1 cut(s) 3113
Vha464I CTTAAG 2 cut(s) 2485, 2596
VpaK11BI GGWCC 3 cut(s) 1549, 3177, 3302
VspI ATTAAT 1 cut(s) 1470
XbaI TCTAGA 1 cut(s) 254
XceI RCATGY 4 cut(s) 634, 1362, 3087, 3226
XcmI CCANNNNNNNNNTGG 2 cut(s) 743, 3245
XmiI GTMKAC 1 cut(s) 1753
XmnI GAANNNNTTC 2 cut(s) 1738, 1911
XspI CTAG 5 cut(s) 255, 1460, 1991, 2355, 2964
ZraI GACGTC 1 cut(s) 1712
ZrmI AGTACT 1 cut(s) 1401
Zsp2I ATGCAT 2 cut(s) 76, 3085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.