RLG00000008845

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000654
Physical Location & Seq
Reverse (-)
40861245 .. 40865764
4520 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008845

Sequence Viewer

Length: 1665 bp
ATGAGCACACAGTTGGATGCTTCTTCTTCTTCTTCCTCGTCCTCCCCTTACCGGTGTACTTATCATGCATTCTTGAGTTTCAGAGGTAAGGACACACGTAAGGGATTTACTGATCATCTTTACAGGGCCTTGGAGCTCGCAGGAATCCACACGTTTAGAGACGATGATGAAATCGAGAGAGGAGCAAATATCGCGGCAGAGTTACAGAGAGCTATCCAAGAGTCGAGAGTATCTGTGGTTGTGTTGTCTAGAGACTATGCTTCATCAAGGTGGTGCTTGGACGAACTTGCAAAGATCATGGAACGTAGGAGAAATGATGACGCACACGTGGTTATTCCAGTGTTTTATGATGTAGATCCTTCCCATGTTAGGAAGCAAATCGGGAGTTTTGCTGAAGCTTTTGTTAGACATGAAGAGCGCTTCAAGGAGGAGATGGGAAAGGTTGCCGAGTGGAGAAGAGCGCTTTCTGATATTGCAGATCTTGCTGGAATGGTTTTAGGACAACGGTATGAATCACAATTTATCCAAGATATTGTTGAAGAAATCAGAAACAACAAGTTGATAGTGCATCATTTGCTTCGAGACATGGCAAGGGGAATTATTCGCGAAGAATCACCTGAGGACCCTGGGAAACGCAGTAGGGTGTGGCACAAAGATGCCTTCAATATTTTGAGTAAAAGAACTGTTGACCTCAAAATAGAGGCATTCAAAAAGATGCACAACCTTGAAGTTCTCCTACTCAATAATGTACATTTCAGTCAAGGCTTTGAAAATTTTCCAAAAAACTTGACATGGCTGTCTTGGCGAGGATTCTCTTTAAATTCGATACCAGCCAGTTTGTATTTGAAAACTCTAGTTGTTCTTGATTTGCGGAATAGCAGCCTGCAATGTGTTTGGAAGGGAACCAAGTTTCTTCCAAGTCTGAAAGTCCTTAATCTTAGTCATTCACATGGCCTTTCAGCAACCCCCGACATATCAGGACTCCCTAACCTTGAGAGATTGATTCTAAAAAATTGTAGAAATTTGATTGAGGTTGATGAATCGATTGGAGACTTGGAGAAACTTGTTTTCTTAAATCTGAAAGACTGCAAAAATCTGATGAAGCTTCCAACCAGAATCAAAATGTCAAGTTCTCTTCAAACACTTATTCTTTCTGGTTGCTCAAAGCTTGTGCTTCATTCTAATATGGTTGCAACAAATCAGTCACTCTTGACAGCTAGTGATATGAAGAAAGACGATTGTTTATCACCTAACTTGTGGAAATCACTCTTATCATGGGCAGTACCGAGCAGAAATATCATACCAACCAGTTTCTCAGTATCAAATTTACCACGTTCTTTACGAAGCTTAAGCCTAGCTGACTGCAACCTATTAGAGATTCCAGGTGATCTTAGTATCCTCTCCTCATTGAAGTATTTAGATCTATGTGGGAACCCAATTCAGAGACTCCCAGAAAACATAAGATCTCTTAGTAAGCTTGAGACACTTATGTTAGAGGACTGCACAGAACTCAGAGCGCTTCCAGAGCTCCCACCAAGTCTCCAGAGTTTGCTTGCAAGTTCTTATAGAGGAAAAAAGGGTAGTTTTAATTTTACATTTGGGGATGCTTCAAGGTGGCTCTGCTGGAAAGAATTCGAGTATATGCAGAATAAATTGCTCTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

555

Amino Acids

63.08

Weight (kDa)

8.69

Isoelectric Point (pI)

56.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 186 3.9e-54 TIR domain
TIR_2 PF13676 24 - 121 1.7e-18 TIR domain
LRR_14 PF23598 445 - 517 4.4e-08 Leucine-rich repeat region
LRR_8 PF13855 445 - 501 1.1e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 796
AccII CGCG 2 cut(s) 194, 606
AciI CCGC 2 cut(s) 194, 871
AclWI GGATC 1 cut(s) 350
AcsI RAATTY 5 cut(s) 772, 820, 1021, 1324, 1631
AcuI CTGAAG 1 cut(s) 414
AcvI CACGTG 1 cut(s) 328
AfaI GTAC 3 cut(s) 58, 750, 1284
AfeI AGCGCT 3 cut(s) 419, 462, 1518
AfiI CCNNNNNNNGG 2 cut(s) 51, 369
AflII CTTAAG 1 cut(s) 1348
AflIII ACRYGT 3 cut(s) 95, 150, 325
AgeI ACCGGT 1 cut(s) 51
AjnI CCWGG 2 cut(s) 625, 1381
Alw21I GWGCWC 3 cut(s) 8, 138, 1530
Alw26I GTCTC 7 cut(s) 153, 246, 576, 1044, 1438, 1475, 1544
AlwI GGATC 1 cut(s) 350
Aor51HI AGCGCT 3 cut(s) 419, 462, 1518
AoxI GGCC 2 cut(s) 126, 952
ApeKI GCWGC 1 cut(s) 879
ApoI RAATTY 5 cut(s) 772, 820, 1021, 1324, 1631
AsiGI ACCGGT 1 cut(s) 51
Asp700I GAANNNNTTC 2 cut(s) 774, 1631
AspLEI GCGC 3 cut(s) 420, 463, 1519
AspS9I GGNCC 2 cut(s) 126, 622
AsuHPI GGTGA 3 cut(s) 606, 1239, 1397
AvaII GGWCC 1 cut(s) 622
AxyI CCTNAGG 1 cut(s) 618
BanII GRGCYC 2 cut(s) 138, 1530
BarI GAAGNNNNNNTAC 2 cut(s) 720, 752
BbrPI CACGTG 1 cut(s) 328
Bbv12I GWGCWC 3 cut(s) 8, 138, 1530
BbvI GCAGC 1 cut(s) 891
BccI CCATC 1 cut(s) 427
BciT130I CCWGG 2 cut(s) 627, 1383
BciVI GTATCC 1 cut(s) 1406
BclI TGATCA 1 cut(s) 112
BcoDI GTCTC 7 cut(s) 153, 246, 576, 1044, 1438, 1475, 1544
BfaI CTAG 5 cut(s) 249, 854, 1218, 1355, 1663
BfoI RGCGCY 3 cut(s) 421, 464, 1520
BfrI CTTAAG 1 cut(s) 1348
BfuI GTATCC 1 cut(s) 1406
BglII AGATCT 3 cut(s) 478, 1420, 1463
BisI GCNGC 2 cut(s) 195, 880
BlsI GCNGC 2 cut(s) 196, 881
Bme1390I CCNGG 2 cut(s) 627, 1383
Bme18I GGWCC 1 cut(s) 622
BmgT120I GGNCC 2 cut(s) 126, 622
BmiI GGNNCC 3 cut(s) 624, 904, 1433
BmrFI CCNGG 2 cut(s) 627, 1383
BmsI GCATC 5 cut(s) 7, 577, 646, 705, 1594
BpmI CTGGAG 1 cut(s) 1526
BpuEI CTTGAG 3 cut(s) 94, 1013, 1499
Bsa29I ATCGAT 1 cut(s) 1043
BsaAI YACGTR 2 cut(s) 98, 328
BsaBI GATNNNNATC 1 cut(s) 354
BsaJI CCNNGG 3 cut(s) 129, 625, 626
BsaWI WCCGGW 1 cut(s) 51
BsaXI ACNNNNNCTCC 2 cut(s) 1524, 1554
Bsc4I CCNNNNNNNGG 2 cut(s) 51, 369
Bse118I RCCGGY 1 cut(s) 51
Bse1I ACTGG 3 cut(s) 338, 834, 1308
Bse21I CCTNAGG 1 cut(s) 618
Bse3DI GCAATG 1 cut(s) 893
Bse8I GATNNNNATC 1 cut(s) 354
BseBI CCWGG 2 cut(s) 627, 1383
BseCI ATCGAT 1 cut(s) 1043
BseDI CCNNGG 3 cut(s) 129, 625, 626
BseGI GGATG 2 cut(s) 22, 1609
BseJI GATNNNNATC 1 cut(s) 354
BseLI CCNNNNNNNGG 2 cut(s) 51, 369
BseMI GCAATG 1 cut(s) 893
BseMII CTCAG 3 cut(s) 609, 1329, 1525
BseNI ACTGG 3 cut(s) 338, 834, 1308
BseRI GAGGAG 3 cut(s) 195, 443, 1393
BseXI GCAGC 1 cut(s) 891
BsgI GTGCAG 1 cut(s) 1486
Bsh1236I CGCG 2 cut(s) 194, 606
BshFI GGCC 2 cut(s) 128, 954
BshTI ACCGGT 1 cut(s) 51
BshVI ATCGAT 1 cut(s) 1043
BsiHKAI GWGCWC 3 cut(s) 8, 138, 1530
BsiSI CCGG 1 cut(s) 52
BslI CCNNNNNNNGG 2 cut(s) 51, 369
BsmAI GTCTC 7 cut(s) 153, 246, 576, 1044, 1438, 1475, 1544
BsmBI CGTCTC 1 cut(s) 153
BsmI GAATGC 2 cut(s) 68, 704
BsnI GGCC 2 cut(s) 128, 954
Bsp1286I GDGCHC 3 cut(s) 8, 138, 1530
Bsp1407I TGTACA 1 cut(s) 748
Bsp143I GATC 7 cut(s) 112, 294, 355, 478, 1387, 1420, 1463
Bsp68I TCGCGA 1 cut(s) 606
BspACI CCGC 2 cut(s) 194, 871
BspANI GGCC 2 cut(s) 128, 954
BspCNI CTCAG 3 cut(s) 610, 1328, 1524
BspDI ATCGAT 1 cut(s) 1043
BspFNI CGCG 2 cut(s) 194, 606
BspLI GGNNCC 3 cut(s) 624, 904, 1433
BspPI GGATC 1 cut(s) 350
BspQI GCTCTTC 2 cut(s) 408, 451
BspTI CTTAAG 1 cut(s) 1348
BsrDI GCAATG 1 cut(s) 893
BsrFI RCCGGY 1 cut(s) 51
BsrGI TGTACA 1 cut(s) 748
BsrI ACTGG 3 cut(s) 338, 834, 1308
BssAI RCCGGY 1 cut(s) 51
BssECI CCNNGG 3 cut(s) 129, 625, 626
BssMI GATC 7 cut(s) 112, 294, 355, 478, 1387, 1420, 1463
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 2 cut(s) 627, 1383
Bst4CI ACNGT 3 cut(s) 12, 507, 685
Bst6I CTCTTC 3 cut(s) 408, 451, 1140
BstAFI CTTAAG 1 cut(s) 1348
BstAPI GCANNNNNTGC 2 cut(s) 482, 574
BstAUI TGTACA 1 cut(s) 748
BstBAI YACGTR 2 cut(s) 98, 328
BstC8I GCNNGC 3 cut(s) 138, 884, 1554
BstDEI CTNAG 6 cut(s) 618, 938, 1315, 1391, 1469, 1511
BstF5I GGATG 2 cut(s) 22, 1609
BstFNI CGCG 2 cut(s) 194, 606
BstH2I RGCGCY 3 cut(s) 421, 464, 1520
BstHHI GCGC 3 cut(s) 420, 463, 1519
BstKTI GATC 7 cut(s) 115, 297, 358, 481, 1390, 1423, 1466
BstMAI GTCTC 7 cut(s) 153, 246, 576, 1044, 1438, 1475, 1544
BstMBI GATC 7 cut(s) 112, 294, 355, 478, 1387, 1420, 1463
BstMWI GCNNNNNNNGC 5 cut(s) 191, 482, 574, 802, 1525
BstNI CCWGG 2 cut(s) 627, 1383
BstSCI CCNGG 2 cut(s) 625, 1381
BstUI CGCG 2 cut(s) 194, 606
BstV1I GCAGC 1 cut(s) 891
BstX2I RGATCY 4 cut(s) 355, 478, 1420, 1463
BstYI RGATCY 4 cut(s) 355, 478, 1420, 1463
Bsu15I ATCGAT 1 cut(s) 1043
Bsu36I CCTNAGG 1 cut(s) 618
BsuI GTATCC 1 cut(s) 1406
BsuRI GGCC 2 cut(s) 128, 954
BsuTUI ATCGAT 1 cut(s) 1043
BtsCI GGATG 2 cut(s) 22, 1609
BtsIMutI CAGTG 1 cut(s) 345
BtuMI TCGCGA 1 cut(s) 606
Cac8I GCNNGC 3 cut(s) 138, 884, 1554
CfoI GCGC 3 cut(s) 420, 463, 1519
Cfr10I RCCGGY 1 cut(s) 51
Cfr13I GGNCC 2 cut(s) 126, 622
ClaI ATCGAT 1 cut(s) 1043
CseI GACGC 1 cut(s) 329
Csp6I GTAC 3 cut(s) 57, 749, 1283
CspAI ACCGGT 1 cut(s) 51
CviAII CATG 8 cut(s) 65, 298, 365, 410, 586, 792, 950, 1275
CviQI GTAC 3 cut(s) 57, 749, 1283
DdeI CTNAG 6 cut(s) 618, 938, 1315, 1391, 1469, 1511
DpnI GATC 7 cut(s) 114, 296, 357, 480, 1389, 1422, 1465
DpnII GATC 7 cut(s) 112, 294, 355, 478, 1387, 1420, 1463
DraI TTTAAA 1 cut(s) 819
DrdI GACNNNNNNGTC 1 cut(s) 796
DseDI GACNNNNNNGTC 1 cut(s) 796
Eam1104I CTCTTC 3 cut(s) 408, 451, 1140
EarI CTCTTC 3 cut(s) 408, 451, 1140
Ecl136II GAGCTC 2 cut(s) 136, 1528
Eco130I CCWWGG 1 cut(s) 129
Eco24I GRGCYC 2 cut(s) 138, 1530
Eco47I GGWCC 1 cut(s) 622
Eco47III AGCGCT 3 cut(s) 419, 462, 1518
Eco53kI GAGCTC 2 cut(s) 136, 1528
Eco57I CTGAAG 1 cut(s) 414
Eco72I CACGTG 1 cut(s) 328
Eco81I CCTNAGG 1 cut(s) 618
EcoICRI GAGCTC 2 cut(s) 136, 1528
EcoO109I RGGNCCY 2 cut(s) 126, 622
EcoRI GAATTC 1 cut(s) 1631
EcoRII CCWGG 2 cut(s) 625, 1381
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 1 cut(s) 70
EcoT38I GRGCYC 2 cut(s) 138, 1530
ErhI CCWWGG 1 cut(s) 129
Esp3I CGTCTC 1 cut(s) 153
FaeI CATG 8 cut(s) 68, 301, 368, 413, 589, 795, 953, 1278
FatI CATG 8 cut(s) 64, 297, 364, 409, 585, 791, 949, 1274
FbaI TGATCA 1 cut(s) 112
Fnu4HI GCNGC 2 cut(s) 195, 880
FokI GGATG 2 cut(s) 29, 1616
FriOI GRGCYC 2 cut(s) 138, 1530
Fsp4HI GCNGC 2 cut(s) 195, 880
FspBI CTAG 5 cut(s) 249, 854, 1218, 1355, 1663
GlaI GCGC 3 cut(s) 419, 462, 1518
GluI GCNGC 2 cut(s) 195, 880
GsuI CTGGAG 1 cut(s) 1526
HaeII RGCGCY 3 cut(s) 421, 464, 1520
HaeIII GGCC 2 cut(s) 128, 954
HapII CCGG 1 cut(s) 52
HgaI GACGC 1 cut(s) 329
HhaI GCGC 3 cut(s) 420, 463, 1519
Hin1II CATG 8 cut(s) 68, 301, 368, 413, 589, 795, 953, 1278
Hin6I GCGC 3 cut(s) 418, 461, 1517
HinP1I GCGC 3 cut(s) 418, 461, 1517
HincII GTYRAC 1 cut(s) 688
HindII GTYRAC 1 cut(s) 688
HindIII AAGCTT 5 cut(s) 396, 1103, 1166, 1345, 1475
HpaII CCGG 1 cut(s) 52
HphI GGTGA 3 cut(s) 606, 1239, 1397
Hpy166II GTNNAC 2 cut(s) 57, 688
Hpy188I TCNGA 8 cut(s) 83, 469, 548, 924, 1080, 1098, 1443, 1514
Hpy8I GTNNAC 2 cut(s) 57, 688
HpyAV CCTTC 3 cut(s) 369, 670, 892
HpyCH4III ACNGT 3 cut(s) 12, 507, 685
HpyCH4IV ACGT 5 cut(s) 97, 152, 304, 327, 1333
HpyF10VI GCNNNNNNNGC 5 cut(s) 191, 482, 574, 802, 1525
HpyF3I CTNAG 6 cut(s) 618, 938, 1315, 1391, 1469, 1511
HpySE526I ACGT 5 cut(s) 97, 152, 304, 327, 1333
Hsp92II CATG 8 cut(s) 68, 301, 368, 413, 589, 795, 953, 1278
HspAI GCGC 3 cut(s) 418, 461, 1517
Ksp22I TGATCA 1 cut(s) 112
Kzo9I GATC 7 cut(s) 112, 294, 355, 478, 1387, 1420, 1463
LguI GCTCTTC 2 cut(s) 408, 451
LmnI GCTCC 3 cut(s) 133, 182, 1533
Lsp1109I GCAGC 1 cut(s) 891
LweI GCATC 5 cut(s) 7, 577, 646, 705, 1594
MaeI CTAG 5 cut(s) 249, 854, 1218, 1355, 1663
MaeII ACGT 5 cut(s) 97, 152, 304, 327, 1333
MaeIII GTNAC 2 cut(s) 201, 1203
MalI GATC 7 cut(s) 114, 296, 357, 480, 1389, 1422, 1465
MboI GATC 7 cut(s) 112, 294, 355, 478, 1387, 1420, 1463
MflI RGATCY 4 cut(s) 355, 478, 1420, 1463
MhlI GDGCHC 3 cut(s) 8, 138, 1530
MlyI GAGTC 3 cut(s) 230, 975, 1440
MmeI TCCRAC 1 cut(s) 1133
Mph1103I ATGCAT 1 cut(s) 70
MroXI GAANNNNTTC 2 cut(s) 774, 1631
MseI TTAA 5 cut(s) 818, 933, 1073, 1349, 1587
MslI CAYNNNNRTG 3 cut(s) 268, 654, 948
MspCI CTTAAG 1 cut(s) 1348
MspI CCGG 1 cut(s) 52
MspR9I CCNGG 2 cut(s) 627, 1383
Mva1269I GAATGC 2 cut(s) 68, 704
MvaI CCWGG 2 cut(s) 627, 1383
MvnI CGCG 2 cut(s) 194, 606
MwoI GCNNNNNNNGC 5 cut(s) 191, 482, 574, 802, 1525
NdeII GATC 7 cut(s) 112, 294, 355, 478, 1387, 1420, 1463
NlaIII CATG 8 cut(s) 68, 301, 368, 413, 589, 795, 953, 1278
NlaIV GGNNCC 3 cut(s) 624, 904, 1433
NmeAIII GCCGAG 1 cut(s) 472
NmuCI GTSAC 1 cut(s) 1203
NruI TCGCGA 1 cut(s) 606
NsiI ATGCAT 1 cut(s) 70
PasI CCCWGGG 1 cut(s) 626
PciSI GCTCTTC 2 cut(s) 408, 451
PcsI WCGNNNNNNNCGW 1 cut(s) 1339
PctI GAATGC 2 cut(s) 68, 704
PdmI GAANNNNTTC 2 cut(s) 774, 1631
PfeI GAWTC 8 cut(s) 144, 512, 611, 810, 1003, 1040, 1116, 1378
PinAI ACCGGT 1 cut(s) 51
PkrI GCNGC 2 cut(s) 196, 881
PleI GAGTC 3 cut(s) 229, 975, 1440
PmaCI CACGTG 1 cut(s) 328
PmlI CACGTG 1 cut(s) 328
PpsI GAGTC 3 cut(s) 229, 975, 1440
Ppu21I YACGTR 2 cut(s) 98, 328
PpuMI RGGWCCY 1 cut(s) 622
Psp124BI GAGCTC 2 cut(s) 138, 1530
Psp5II RGGWCCY 1 cut(s) 622
Psp6I CCWGG 2 cut(s) 625, 1381
PspCI CACGTG 1 cut(s) 328
PspGI CCWGG 2 cut(s) 625, 1381
PspN4I GGNNCC 3 cut(s) 624, 904, 1433
PspPI GGNCC 2 cut(s) 126, 622
PspPPI RGGWCCY 1 cut(s) 622
PsuI RGATCY 4 cut(s) 355, 478, 1420, 1463
RruI TCGCGA 1 cut(s) 606
RsaI GTAC 3 cut(s) 58, 750, 1284
RsaNI GTAC 3 cut(s) 57, 749, 1283
RseI CAYNNNNRTG 3 cut(s) 268, 654, 948
SacI GAGCTC 2 cut(s) 138, 1530
SapI GCTCTTC 2 cut(s) 408, 451
SaqAI TTAA 5 cut(s) 818, 933, 1073, 1349, 1587
SatI GCNGC 2 cut(s) 195, 880
Sau3AI GATC 7 cut(s) 112, 294, 355, 478, 1387, 1420, 1463
Sau96I GGNCC 2 cut(s) 126, 622
SchI GAGTC 3 cut(s) 230, 975, 1440
ScrFI CCNGG 2 cut(s) 627, 1383
SduI GDGCHC 3 cut(s) 8, 138, 1530
SfaNI GCATC 5 cut(s) 7, 577, 646, 705, 1594
SinI GGWCC 1 cut(s) 622
SmiMI CAYNNNNRTG 3 cut(s) 268, 654, 948
SmlI CTYRAG 4 cut(s) 73, 992, 1348, 1478
SmoI CTYRAG 4 cut(s) 73, 992, 1348, 1478
SsiI CCGC 2 cut(s) 194, 871
SspI AATATT 1 cut(s) 667
SspMI CTAG 5 cut(s) 249, 854, 1218, 1355, 1663
SstI GAGCTC 2 cut(s) 138, 1530
StyD4I CCNGG 2 cut(s) 625, 1381
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 3 cut(s) 12, 507, 685
TaiI ACGT 5 cut(s) 100, 155, 307, 330, 1336
TaqI TCGA 6 cut(s) 174, 224, 580, 824, 1043, 1635
TatI WGTACW 2 cut(s) 56, 748
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 8 cut(s) 144, 512, 611, 810, 1003, 1040, 1116, 1378
Tru1I TTAA 5 cut(s) 818, 933, 1073, 1349, 1587
Tru9I TTAA 5 cut(s) 818, 933, 1073, 1349, 1587
TscAI CASTG 1 cut(s) 345
TseFI GTSAC 1 cut(s) 1203
TseI GCWGC 1 cut(s) 879
Tsp45I GTSAC 1 cut(s) 1203
TspDTI ATGAA 8 cut(s) 183, 252, 426, 525, 1053, 1115, 1166, 1241
TspRI CASTG 1 cut(s) 345
Vha464I CTTAAG 1 cut(s) 1348
VpaK11BI GGWCC 1 cut(s) 622
XapI RAATTY 5 cut(s) 772, 820, 1021, 1324, 1631
XbaI TCTAGA 1 cut(s) 248
XmnI GAANNNNTTC 2 cut(s) 774, 1631
XspI CTAG 5 cut(s) 249, 854, 1218, 1355, 1663
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.