MD17G1276000.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000654
Physical Location & Seq
Forward (+)
33679734 .. 33682698
2965 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1276000.v1.1.491

Sequence Viewer

Length: 549 bp
ATGCTGTCCTCGTTGGAGAAGTTGGATCTGAGTTGCAATCCGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAAACTCTTAAAGTATCTGGTTGCAGAAACCTCACAATACTTCCAGAGCTCCCACATTCTTTGACAAGTTTAACACTGGCTTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCTGTCCTCGTTGGAGAAGTTGGATCTGAGTTACAATCCGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAGACTCTTAAAGTATCTGGTTGCAGAAACCTCACAATACTTCCAGAGCTCCCACGTTCTTTGACAAGTTTAACACTGGCTTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTATGCTGTCCTCATTAGAGTACTTGGATCTTGATGGTAATCTGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAGACTCTTAAAGTATCTGGTTGCAAAAACCTCACAATGCTTCCAGAGCTCCCGCGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

183

Amino Acids

19.79

Weight (kDa)

5.42

Isoelectric Point (pI)

55.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 3 - 75 4.1e-07 Leucine-rich repeat region
LRR_4 PF12799 4 - 47 1.1e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 48 - 104 2.4e-10 Leucine rich repeat
LRR_4 PF12799 48 - 86 1.1e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 50 - 119 7.1e-08 Leucine-rich repeat region
LRR_4 PF12799 71 - 113 5e-07 Leucine Rich repeats (2 copies)
LRR_8 PF13855 114 - 170 2.1e-10 Leucine rich repeat
LRR_4 PF12799 115 - 153 2.2e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 118 - 178 3.8e-07 Leucine-rich repeat region
LRR_4 PF12799 138 - 180 6.9e-06 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 541
AciI CCGC 1 cut(s) 539
AclWI GGATC 3 cut(s) 33, 234, 435
AfaI GTAC 1 cut(s) 422
AluBI AGCT 6 cut(s) 82, 133, 283, 334, 484, 535
AluI AGCT 6 cut(s) 82, 133, 283, 334, 484, 535
Alw21I GWGCWC 3 cut(s) 135, 336, 537
AlwI GGATC 3 cut(s) 33, 234, 435
ApeKI GCWGC 2 cut(s) 168, 369
BaeGI GKGCMC 2 cut(s) 196, 397
BanII GRGCYC 3 cut(s) 135, 336, 537
Bbv12I GWGCWC 3 cut(s) 135, 336, 537
BbvI GCAGC 2 cut(s) 155, 356
BccI CCATC 1 cut(s) 428
BisI GCNGC 2 cut(s) 169, 370
BlsI GCNGC 2 cut(s) 170, 371
BmcAI AGTACT 1 cut(s) 422
BpmI CTGGAG 2 cut(s) 269, 470
BsaBI GATNNNNATC 1 cut(s) 438
Bse1I ACTGG 2 cut(s) 165, 366
Bse8I GATNNNNATC 1 cut(s) 438
BseJI GATNNNNATC 1 cut(s) 438
BseMII CTCAG 2 cut(s) 20, 221
BseNI ACTGG 2 cut(s) 165, 366
BseSI GKGCMC 2 cut(s) 196, 397
BseXI GCAGC 2 cut(s) 155, 356
Bsh1236I CGCG 1 cut(s) 541
BsiHKAI GWGCWC 3 cut(s) 135, 336, 537
Bsp1286I GDGCHC 5 cut(s) 135, 196, 336, 397, 537
Bsp143I GATC 3 cut(s) 25, 226, 427
BspACI CCGC 1 cut(s) 539
BspCNI CTCAG 2 cut(s) 21, 222
BspFNI CGCG 1 cut(s) 541
BspPI GGATC 3 cut(s) 33, 234, 435
BsrI ACTGG 2 cut(s) 165, 366
BssMI GATC 3 cut(s) 25, 226, 427
BstC8I GCNNGC 2 cut(s) 166, 367
BstDEI CTNAG 2 cut(s) 29, 230
BstFNI CGCG 1 cut(s) 541
BstKTI GATC 3 cut(s) 28, 229, 430
BstMBI GATC 3 cut(s) 25, 226, 427
BstMWI GCNNNNNNNGC 1 cut(s) 532
BstSLI GKGCMC 2 cut(s) 196, 397
BstUI CGCG 1 cut(s) 541
BstV1I GCAGC 2 cut(s) 155, 356
BstX2I RGATCY 3 cut(s) 25, 226, 427
BstYI RGATCY 3 cut(s) 25, 226, 427
BtsIMutI CAGTG 2 cut(s) 158, 359
Cac8I GCNNGC 2 cut(s) 166, 367
Csp6I GTAC 1 cut(s) 421
CspCI CAANNNNNGTGG 4 cut(s) 126, 161, 327, 362
CviAII CATG 3 cut(s) 64, 265, 466
CviQI GTAC 1 cut(s) 421
DdeI CTNAG 2 cut(s) 29, 230
DpnI GATC 3 cut(s) 27, 228, 429
DpnII GATC 3 cut(s) 25, 226, 427
Ecl136II GAGCTC 3 cut(s) 133, 334, 535
Eco24I GRGCYC 3 cut(s) 135, 336, 537
Eco53kI GAGCTC 3 cut(s) 133, 334, 535
EcoICRI GAGCTC 3 cut(s) 133, 334, 535
EcoT38I GRGCYC 3 cut(s) 135, 336, 537
FaeI CATG 3 cut(s) 67, 268, 469
FaiI YATR 7 cut(s) 65, 176, 203, 266, 377, 404, 467
FatI CATG 3 cut(s) 63, 264, 465
FauI CCCGC 1 cut(s) 546
Fnu4HI GCNGC 2 cut(s) 169, 370
FriOI GRGCYC 3 cut(s) 135, 336, 537
Fsp4HI GCNGC 2 cut(s) 169, 370
GluI GCNGC 2 cut(s) 169, 370
GsuI CTGGAG 2 cut(s) 269, 470
Hin1II CATG 3 cut(s) 67, 268, 469
HinfI GANTC 4 cut(s) 184, 289, 385, 490
Hpy188I TCNGA 7 cut(s) 30, 42, 181, 231, 243, 382, 444
Hpy188III TCNNGA 6 cut(s) 128, 286, 329, 431, 487, 530
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 6 cut(s) 36, 108, 171, 309, 372, 510
HpyF10VI GCNNNNNNNGC 1 cut(s) 532
HpyF3I CTNAG 2 cut(s) 29, 230
HpySE526I ACGT 1 cut(s) 340
Hsp92II CATG 3 cut(s) 67, 268, 469
Kzo9I GATC 3 cut(s) 25, 226, 427
LmnI GCTCC 6 cut(s) 87, 138, 288, 339, 489, 540
Lsp1109I GCAGC 2 cut(s) 155, 356
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 1 cut(s) 233
MalI GATC 3 cut(s) 27, 228, 429
MboI GATC 3 cut(s) 25, 226, 427
MflI RGATCY 3 cut(s) 25, 226, 427
MhlI GDGCHC 5 cut(s) 135, 196, 336, 397, 537
MlyI GAGTC 2 cut(s) 283, 484
MmeI TCCRAC 2 cut(s) 195, 204
MnlI CCTC 6 cut(s) 19, 125, 220, 326, 421, 527
MseI TTAA 8 cut(s) 78, 93, 155, 279, 294, 356, 480, 495
MvnI CGCG 1 cut(s) 541
MwoI GCNNNNNNNGC 1 cut(s) 532
NdeII GATC 3 cut(s) 25, 226, 427
NlaIII CATG 3 cut(s) 67, 268, 469
PfeI GAWTC 2 cut(s) 184, 385
PkrI GCNGC 2 cut(s) 170, 371
PleI GAGTC 2 cut(s) 283, 484
PpsI GAGTC 2 cut(s) 283, 484
Psp124BI GAGCTC 3 cut(s) 135, 336, 537
PsuI RGATCY 3 cut(s) 25, 226, 427
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
SacI GAGCTC 3 cut(s) 135, 336, 537
SaqAI TTAA 8 cut(s) 78, 93, 155, 279, 294, 356, 480, 495
SatI GCNGC 2 cut(s) 169, 370
Sau3AI GATC 3 cut(s) 25, 226, 427
ScaI AGTACT 1 cut(s) 422
SchI GAGTC 2 cut(s) 283, 484
SduI GDGCHC 5 cut(s) 135, 196, 336, 397, 537
SsiI CCGC 1 cut(s) 539
SstI GAGCTC 3 cut(s) 135, 336, 537
TaiI ACGT 1 cut(s) 343
TatI WGTACW 1 cut(s) 420
TfiI GAWTC 2 cut(s) 184, 385
Tru1I TTAA 8 cut(s) 78, 93, 155, 279, 294, 356, 480, 495
Tru9I TTAA 8 cut(s) 78, 93, 155, 279, 294, 356, 480, 495
TscAI CASTG 2 cut(s) 165, 366
TseI GCWGC 2 cut(s) 168, 369
TspDTI ATGAA 3 cut(s) 80, 281, 482
TspRI CASTG 2 cut(s) 165, 366
ZrmI AGTACT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.