FvH4_2g20310

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000654
Physical Location & Seq
Reverse (-)
17084464 .. 17089230
4767 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20310.t1

Sequence Viewer

Length: 3339 bp
ATGGAACGTCAAATAAACGATGGATATATGGTAGTCCCCGTTTTCTATAAGGTGGATCCATCTGTTGTTAGGTGGCAGAGTGGGTGTTTTGCTGAAGCATTTGTCAAACATGAAGAACGCTTCAAGGCGGAGATGGACAAGGTGACCAAGTGGAGAAAAGCTCTCAAAGCTGCCGCCGATCTAAGAGGGGTGGTCTTAGGAGATCAGTACGAATCACAGGTCATCCAAAATATTGTTGACGATATTGAAAAGAAAATGGATCCCGTTACTTTAAATGTTGCCCCATATGCTGTTGGAATAAACGATCGGGTGGAAGACCTCAACATGTGGTTAAGAGATGGATCAGTTGATGTTGGTGTGGCTGGCATCTATGGGATGGGTGGAATTGGCAAGACCACTATTGCTAAAGCTGCTTATAATCTGAACCAGCATAGTTTTGAAGGAAGAAGCTTTCTGGCAGATGTTAGAGCAACTACAGAACAACCTGATGGTTTGGTTCGCTTGCAGAGGAACCTTCTGTCAGATATCCAAAGGAGAAGAGCAAAGAAGATTAACAATGTGGCTGAAGGTACGAGCAAAATTAAGGATGTTGTACGCTGCAAAAGAGTACTGATTGTTCTTGATGATGTGAACGACTTGGACCAGTTAAATGCGATTTTTGGAATGCGCGAGTGGTTTCTTCCCGGAAGCAAGATTATCATAACAACTAGAGATAAACACTTGTTAACGGCTAATGGGGTTTCAAAAATGTTCATGGTCCCAGAATTGGATGAGGATGAATCACTTCAGCTTTTCAGTTGGCATGCCTTCAGACAAGCTCATCCTATCGAAGGCTTCATGGAGGTTTCAGCACTCGTAGTAAAACACTGCGGAGGGCTACCGTTAGCCCTCCAAGTTCTGGGGTCTTCTCTGTTTGGAAAAAGTCTACTTGTATGGTACGATACATTAGAGAGGTTAGATGAGGTTCCTGATGGAAAAATTCAAAGAATTCTTAGAATAAGCTATGATTATCTACAAGACGATCGTGACAAAACTTTATTCCTTCATATAGCATGTTTCTTCATAGGAAAAAAGAGAAACTTGACAGTCACAGTACTGCACCATCTAAAGCTTTTCGCAACAAGTGGAGTTCAAAATCTAGTTGATAGATGTCTACTGAAAATTGATGATGATAAGTTGATGATGCATCAATTAATCAGAGACATGGGAAGGGGAATTGTTTGCGAAGAATCAGTTGATGACCCTGGAAAACGCAGTAGAGTTGTGCAAAAAGATGCCTATGAGATTTTGAAAAACTCATCGGGAACGGAAACTATCAAGGGTCTCTTGCTCAACTTCTCTATGCTAATGGATGGCATGTCTTCCACGGCAATACTTAAAGATAACAATAAACGATGGCATGCCGAGGAGTCTGATGGAACTTTTTCAAGACGGCGTCGGCTTAGCTTCTTCTCTTGGGAATCAATCAACTATTCCTCACATAATTTAGCTTCTACATCGAGTGTGGGAGACTTCAAAACAGAGGCATTCAAAAGGATGACCAATCTTCAACTTCTCCAGCTCAATAATGTAAAAGTCAATGGTGGCTATAGAGAGTTTCCAAAAGCACTAATATGGTTGTCTTGGAAAGGGTTCCCCATGAAATCAATGCCAGCTGATTTTTGTTTGAAAAATCTAGTTGCTCTTGAGTTGAAGCATAGCATCCTGCAACTTGTATGGAAGGGAATCAAGTTTATTCCAGGATTGAAAATCCTTGATCTCAGTCATTCACATGGTCTTGTTAGAACCCCTGACTTCTCCGGACTCCCTACCCTTGAGAGATTAATTCTTAAAGATTGCATAAATATTGTCGAGATTGATGAATCAATCGGAGAACTTCAGGAACTTGTTTTCTTGAATCTAAAAGACTGCAAAAATCTCATGAAGCTTCCAAGAAGAATTAGCATGTTGACATCTCTTCATGAGCTAATCCTCTCTGGTTGCTCAAAGCTTGGTGATACAGTGAAACTCGGTCCTTCATCAAATAAACCATGGCTATCAATCTGTTCGTGGGTATCACCAAGAAAAAGTCTTGAAGCAACTAGCAGCTTCTCGTTGGCACATTTGCCAACATCTTTAGGAAGCTTAAGTCTGGCTGGTTGCAATCTATCAAAGATTGCTGATGATCTAAGTGTTCTATCCTCGCTGAAGTATTTGGATGTAAGTGGCAACCCAATTGTGTGCCTACCCGAGAACCTAAAAAGTCTTACTATGCTAGAATCTCTTTCCGTAAACGACTGCACAAAGCTTAAAATGCTTCCGGAGCTCCCTCCAAGTTTAAAGAATTTGCAAGCAGAAGTTTGTACTTCGTTGAGAAAAGTAACGAACTTACCAAACTTTTTCATGTCCCTAACACCATTTCTTACAGATTGCGGAGAATTAGCTGAAGTTCAGAGCTTGTTCACTATAAAACCATTGAGAGGCACTGACTTACAGATGATAAAAAATATGGGACTGTGCAATTTGGAATCCATCAGAGGCATTGAAGTCGAAATGGGCAATCACTTGACGTATACATCCAGGACAGGTCCCCTCCAGGGACTATATGAATGTGGTATATTCAGCATTTTCCTTCACGCGCACACAATTCCTGATCAGTACATCTACAGGAACTTGGGTAGTTCTGTGTTGTCGATTATTGTACCTTCCGATCCTAAGCTTAAGATCCGAGGCTTAAATGTATGTGTTCTATATGCACTAGGCTTATATAGGAGTTATGCTGGTTCCCAGATTCTTAAAGTCAGTAACGAGACGAAGGGTCTTATGTGGACATACTGCCCAGTTACTCAAGGTGTTCCGAAAGAGGATGGACTAATGTTATGGCTAAGTCATTGGCTGTTTGAGAACAATGAATTGGAGGGTGGGGATGAAATACGTGTCTCAGTGCATAGTGCAGCTGCGTTCATGCATTTTCCAGAAGGTCAAGACAGTCTTCCCGCAAAGAAGTTTGGAATTCAACTTGTGTATGATCAGGAAAATGAGGGTGTTCCATCCAATGGTCAAGAAATTGCACTACAAGATGAAGTGCCTTGTTGGAGCCACAATGTCATCACTGGGAGTGTGTCATTGTCAGCATATATGTATCAAATGTGGCCGGGCAAATACTTTATTTGCAATAATGTTAACTACATGCGTCATGGACATTTCAGAAGCTGCAAGGAGAATCCATCTCATCTTGATTTTTCATACGAGCCCAAAGATCGTGTGAGCGAGTATTGCCATCATTTATTTAAGCACGCTATCTTTTCACAAGAGAAAATTGAGTACATATCGAGTAAAAATGACACTTTATCTGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1113

Amino Acids

125.33

Weight (kDa)

7.33

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 3 - 89 2.6e-13 TIR domain
NB-ARC PF00931 102 - 272 5.5e-22 NB-ARC domain
WHD_ROQ1 PF23282 341 - 411 9.1e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 709 - 798 7e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 417
AccB7I CCANNNNNTGG 2 cut(s) 898, 3035
AccI GTMKAC 3 cut(s) 925, 1153, 2552
AccII CGCG 2 cut(s) 669, 2618
AccIII TCCGGA 2 cut(s) 1799, 2299
AciI CCGC 5 cut(s) 128, 174, 870, 2412, 2976
AclWI GGATC 7 cut(s) 50, 63, 254, 267, 349, 2684, 2698
AcoI YGGCCR 1 cut(s) 3131
AcsI RAATTY 4 cut(s) 978, 987, 2323, 2991
AcuI CTGAAG 7 cut(s) 114, 585, 770, 793, 1862, 2207, 2445
AcyI GRCGYC 1 cut(s) 1435
AfiI CCNNNNNNNGG 6 cut(s) 766, 830, 898, 2459, 2576, 3035
AflII CTTAAG 2 cut(s) 2125, 2699
AflIII ACRYGT 2 cut(s) 324, 2914
AhdI GACNNNNNGTC 1 cut(s) 2796
AjnI CCWGG 4 cut(s) 1243, 1738, 2558, 2574
AjuI GAANNNNNNNTTGG 2 cut(s) 2876, 2908
Alw21I GWGCWC 1 cut(s) 2307
Alw26I GTCTC 5 cut(s) 1194, 1328, 1503, 2783, 2923
AlwI GGATC 7 cut(s) 50, 63, 254, 267, 349, 2684, 2698
AlwNI CAGNNNCTG 1 cut(s) 3192
Ama87I CYCGRG 1 cut(s) 2228
Aor13HI TCCGGA 2 cut(s) 1799, 2299
AoxI GGCC 1 cut(s) 3131
ApeKI GCWGC 7 cut(s) 170, 410, 597, 2085, 2933, 2936, 3192
ApoI RAATTY 4 cut(s) 978, 987, 2323, 2991
ArsI GACNNNNNNTTYG 2 cut(s) 204, 236
AseI ATTAAT 2 cut(s) 1193, 1823
Asp700I GAANNNNTTC 3 cut(s) 783, 1423, 1631
AspLEI GCGC 2 cut(s) 669, 2620
AspS9I GGNCC 4 cut(s) 640, 757, 2012, 2567
AsuC2I CCSGG 2 cut(s) 684, 3135
AsuHPI GGTGA 3 cut(s) 154, 2006, 2049
AvaI CYCGRG 1 cut(s) 2228
AvaII GGWCC 4 cut(s) 640, 757, 2012, 2567
BamHI GGATCC 2 cut(s) 55, 259
BanII GRGCYC 2 cut(s) 2307, 3234
BbsI GAAGAC 4 cut(s) 321, 897, 1353, 2963
Bbv12I GWGCWC 1 cut(s) 2307
BbvI GCAGC 7 cut(s) 157, 397, 584, 2097, 2923, 2945, 3179
BceAI ACGGC 3 cut(s) 744, 1383, 1448
BcgI CGANNNNNNTGC 2 cut(s) 2509, 2543
BciT130I CCWGG 4 cut(s) 1245, 1740, 2560, 2576
BclI TGATCA 2 cut(s) 2632, 3007
BcnI CCSGG 2 cut(s) 684, 3135
BcoDI GTCTC 5 cut(s) 1194, 1328, 1503, 2783, 2923
BfaI CTAG 6 cut(s) 708, 1139, 1676, 2082, 2255, 2738
BfmI CTRYAG 3 cut(s) 474, 1588, 2644
BfrI CTTAAG 2 cut(s) 2125, 2699
BisI GCNGC 8 cut(s) 171, 174, 411, 598, 2086, 2934, 2937, 3193
BlpI GCTNAGC 1 cut(s) 1442
BlsI GCNGC 8 cut(s) 172, 175, 412, 599, 2087, 2935, 2938, 3194
BmcAI AGTACT 2 cut(s) 609, 1095
Bme1390I CCNGG 6 cut(s) 684, 1245, 1740, 2560, 2576, 3135
Bme18I GGWCC 4 cut(s) 640, 757, 2012, 2567
BmeRI GACNNNNNGTC 1 cut(s) 2796
BmeT110I CYCGRG 1 cut(s) 2228
BmgT120I GGNCC 4 cut(s) 640, 757, 2012, 2567
BmiI GGNNCC 9 cut(s) 57, 261, 512, 759, 966, 1634, 2569, 2764, 3077
BmrFI CCNGG 6 cut(s) 684, 1245, 1740, 2560, 2576, 3135
BmrI ACTGGG 2 cut(s) 2813, 3102
BmsI GCATC 5 cut(s) 375, 1173, 1195, 1264, 1710
BmuI ACTGGG 2 cut(s) 2813, 3102
BpiI GAAGAC 4 cut(s) 321, 897, 1353, 2963
BplI GAGNNNNNCTC 2 cut(s) 145, 177
BpmI CTGGAG 2 cut(s) 1541, 2558
Bpu10I CCTNAGC 1 cut(s) 2694
Bpu1102I GCTNAGC 1 cut(s) 1442
BpuEI CTTGAG 3 cut(s) 1706, 1835, 2811
BpuMI CCSGG 2 cut(s) 684, 3135
BsaAI YACGTR 1 cut(s) 2915
BsaHI GRCGYC 1 cut(s) 1435
BsaI GGTCTC 1 cut(s) 1328
BsaJI CCNNGG 6 cut(s) 1243, 1365, 1404, 2030, 2575, 2707
BsaWI WCCGGW 2 cut(s) 1799, 2299
Bsc4I CCNNNNNNNGG 6 cut(s) 766, 830, 898, 2459, 2576, 3035
Bse1I ACTGG 3 cut(s) 643, 2819, 3097
BseAI TCCGGA 2 cut(s) 1799, 2299
BseBI CCWGG 4 cut(s) 1245, 1740, 2560, 2576
BseDI CCNNGG 6 cut(s) 1243, 1365, 1404, 2030, 2575, 2707
BseLI CCNNNNNNNGG 6 cut(s) 766, 830, 898, 2459, 2576, 3035
BseMII CTCAG 2 cut(s) 1774, 2934
BseNI ACTGG 3 cut(s) 643, 2819, 3097
BseRI GAGGAG 1 cut(s) 1421
BseXI GCAGC 7 cut(s) 157, 397, 584, 2097, 2923, 2945, 3179
BsgI GTGCAG 3 cut(s) 1082, 2263, 2952
Bsh1236I CGCG 2 cut(s) 669, 2618
Bsh1285I CGRYCG 2 cut(s) 307, 1024
BshFI GGCC 1 cut(s) 3133
BsiEI CGRYCG 2 cut(s) 307, 1024
BsiHKAI GWGCWC 1 cut(s) 2307
BsiHKCI CYCGRG 1 cut(s) 2228
BsiSI CCGG 4 cut(s) 684, 1800, 2300, 3134
BslFI GGGAC 6 cut(s) 20, 743, 2371, 2505, 2553, 2592
BslI CCNNNNNNNGG 6 cut(s) 766, 830, 898, 2459, 2576, 3035
BsmAI GTCTC 5 cut(s) 1194, 1328, 1503, 2783, 2923
BsmBI CGTCTC 1 cut(s) 2783
BsmFI GGGAC 6 cut(s) 20, 743, 2371, 2505, 2553, 2592
BsmI GAATGC 2 cut(s) 669, 1526
BsnI GGCC 1 cut(s) 3133
Bso31I GGTCTC 1 cut(s) 1328
BsoBI CYCGRG 1 cut(s) 2228
Bsp1286I GDGCHC 2 cut(s) 2307, 3234
Bsp13I TCCGGA 2 cut(s) 1799, 2299
Bsp1720I GCTNAGC 1 cut(s) 1442
Bsp19I CCATGG 1 cut(s) 2030
BspACI CCGC 5 cut(s) 128, 174, 870, 2412, 2976
BspANI GGCC 1 cut(s) 3133
BspCNI CTCAG 2 cut(s) 1773, 2933
BspEI TCCGGA 2 cut(s) 1799, 2299
BspFNI CGCG 2 cut(s) 669, 2618
BspHI TCATGA 2 cut(s) 1920, 1960
BspLI GGNNCC 9 cut(s) 57, 261, 512, 759, 966, 1634, 2569, 2764, 3077
BspPI GGATC 7 cut(s) 50, 63, 254, 267, 349, 2684, 2698
BspQI GCTCTTC 1 cut(s) 532
BspTI CTTAAG 2 cut(s) 2125, 2699
BspTNI GGTCTC 1 cut(s) 1328
BsrI ACTGG 3 cut(s) 643, 2819, 3097
BssECI CCNNGG 6 cut(s) 1243, 1365, 1404, 2030, 2575, 2707
BssNAI GTATAC 1 cut(s) 2553
BssNI GRCGYC 1 cut(s) 1435
BssT1I CCWWGG 1 cut(s) 2030
Bst1107I GTATAC 1 cut(s) 2553
Bst2UI CCWGG 4 cut(s) 1245, 1740, 2560, 2576
Bst4CI ACNGT 6 cut(s) 882, 1087, 1093, 2002, 2496, 2969
Bst6I CTCTTC 2 cut(s) 532, 1962
BstACI GRCGYC 1 cut(s) 1435
BstAFI CTTAAG 2 cut(s) 2125, 2699
BstBAI YACGTR 1 cut(s) 2915
BstC8I GCNNGC 7 cut(s) 364, 503, 804, 1401, 1653, 2331, 3276
BstDEI CTNAG 9 cut(s) 182, 196, 992, 1442, 1760, 2168, 2694, 2864, 2920
BstDSI CCRYGG 2 cut(s) 1365, 2030
BstEII GGTNACC 1 cut(s) 142
BstENI CCTNNNNNAGG 1 cut(s) 828
BstFNI CGCG 2 cut(s) 669, 2618
BstHHI GCGC 2 cut(s) 669, 2620
BstMAI GTCTC 5 cut(s) 1194, 1328, 1503, 2783, 2923
BstMCI CGRYCG 2 cut(s) 307, 1024
BstMWI GCNNNNNNNGC 6 cut(s) 167, 287, 410, 2293, 2302, 3255
BstNI CCWGG 4 cut(s) 1245, 1740, 2560, 2576
BstNSI RCATGY 7 cut(s) 328, 806, 1056, 1360, 1403, 1948, 3172
BstPI GGTNACC 1 cut(s) 142
BstSCI CCNGG 6 cut(s) 682, 1243, 1738, 2558, 2574, 3133
BstSFI CTRYAG 3 cut(s) 474, 1588, 2644
BstUI CGCG 2 cut(s) 669, 2618
BstV1I GCAGC 7 cut(s) 157, 397, 584, 2097, 2923, 2945, 3179
BstV2I GAAGAC 4 cut(s) 321, 897, 1353, 2963
BstX2I RGATCY 3 cut(s) 55, 259, 2703
BstYI RGATCY 3 cut(s) 55, 259, 2703
BstZ17I GTATAC 1 cut(s) 2553
BsuRI GGCC 1 cut(s) 3133
BtgI CCRYGG 2 cut(s) 1365, 2030
BtsI GCAGTG 1 cut(s) 865
BtsIMutI CAGTG 5 cut(s) 865, 2007, 2463, 2928, 3090
Cac8I GCNNGC 7 cut(s) 364, 503, 804, 1401, 1653, 2331, 3276
CaiI CAGNNNCTG 1 cut(s) 3192
CciI TCATGA 2 cut(s) 1920, 1960
CfoI GCGC 2 cut(s) 669, 2620
Cfr13I GGNCC 4 cut(s) 640, 757, 2012, 2567
CseI GACGC 2 cut(s) 1424, 3161
DdeI CTNAG 9 cut(s) 182, 196, 992, 1442, 1760, 2168, 2694, 2864, 2920
DraI TTTAAA 2 cut(s) 273, 2319
DriI GACNNNNNGTC 1 cut(s) 2796
EaeI YGGCCR 1 cut(s) 3131
Eam1104I CTCTTC 2 cut(s) 532, 1962
Eam1105I GACNNNNNGTC 1 cut(s) 2796
EarI CTCTTC 2 cut(s) 532, 1962
EciI GGCGGA 1 cut(s) 143
Ecl136II GAGCTC 1 cut(s) 2305
Eco130I CCWWGG 1 cut(s) 2030
Eco24I GRGCYC 2 cut(s) 2307, 3234
Eco31I GGTCTC 1 cut(s) 1328
Eco32I GATATC 1 cut(s) 526
Eco47I GGWCC 4 cut(s) 640, 757, 2012, 2567
Eco53kI GAGCTC 1 cut(s) 2305
Eco57I CTGAAG 7 cut(s) 114, 585, 770, 793, 1862, 2207, 2445
Eco88I CYCGRG 1 cut(s) 2228
Eco91I GGTNACC 1 cut(s) 142
EcoICRI GAGCTC 1 cut(s) 2305
EcoNI CCTNNNNNAGG 1 cut(s) 828
EcoO109I RGGNCCY 1 cut(s) 2567
EcoO65I GGTNACC 1 cut(s) 142
EcoRI GAATTC 2 cut(s) 987, 2991
EcoRII CCWGG 4 cut(s) 1243, 1738, 2558, 2574
EcoRV GATATC 1 cut(s) 526
EcoT14I CCWWGG 1 cut(s) 2030
EcoT22I ATGCAT 2 cut(s) 1188, 2949
EcoT38I GRGCYC 2 cut(s) 2307, 3234
ErhI CCWWGG 1 cut(s) 2030
Esp3I CGTCTC 1 cut(s) 2783
FalI AAGNNNNNCTT 6 cut(s) 1064, 1096, 1310, 1342, 2955, 2987
FaqI GGGAC 6 cut(s) 20, 743, 2371, 2505, 2553, 2592
FauI CCCGC 1 cut(s) 2983
FauNDI CATATG 1 cut(s) 286
FbaI TGATCA 2 cut(s) 2632, 3007
FblI GTMKAC 3 cut(s) 925, 1153, 2552
Fnu4HI GCNGC 8 cut(s) 171, 174, 411, 598, 2086, 2934, 2937, 3193
FriOI GRGCYC 2 cut(s) 2307, 3234
Fsp4HI GCNGC 8 cut(s) 171, 174, 411, 598, 2086, 2934, 2937, 3193
FspBI CTAG 6 cut(s) 708, 1139, 1676, 2082, 2255, 2738
GlaI GCGC 2 cut(s) 668, 2619
GluI GCNGC 8 cut(s) 171, 174, 411, 598, 2086, 2934, 2937, 3193
GsuI CTGGAG 2 cut(s) 1541, 2558
HaeIII GGCC 1 cut(s) 3133
HapII CCGG 4 cut(s) 684, 1800, 2300, 3134
HgaI GACGC 2 cut(s) 1424, 3161
HhaI GCGC 2 cut(s) 669, 2620
Hin1I GRCGYC 1 cut(s) 1435
Hin6I GCGC 2 cut(s) 667, 2618
HinP1I GCGC 2 cut(s) 667, 2618
HincII GTYRAC 4 cut(s) 238, 726, 1950, 3163
HindII GTYRAC 4 cut(s) 238, 726, 1950, 3163
HindIII AAGCTT 7 cut(s) 448, 1109, 1925, 1988, 2122, 2285, 2696
HpaI GTTAAC 2 cut(s) 726, 3163
HpaII CCGG 4 cut(s) 684, 1800, 2300, 3134
HphI GGTGA 3 cut(s) 154, 2006, 2049
Hpy99I CGWCG 1 cut(s) 1440
HpyCH4III ACNGT 6 cut(s) 882, 1087, 1093, 2002, 2496, 2969
HpyCH4IV ACGT 3 cut(s) 7, 2549, 2914
HpyF10VI GCNNNNNNNGC 6 cut(s) 167, 287, 410, 2293, 2302, 3255
HpyF3I CTNAG 9 cut(s) 182, 196, 992, 1442, 1760, 2168, 2694, 2864, 2920
HpySE526I ACGT 3 cut(s) 7, 2549, 2914
Hsp92I GRCGYC 1 cut(s) 1435
HspAI GCGC 2 cut(s) 667, 2618
Kpn2I TCCGGA 2 cut(s) 1799, 2299
Ksp22I TGATCA 2 cut(s) 2632, 3007
KspAI GTTAAC 2 cut(s) 726, 3163
LguI GCTCTTC 1 cut(s) 532
LmnI GCTCC 3 cut(s) 2302, 2310, 3075
Lsp1109I GCAGC 7 cut(s) 157, 397, 584, 2097, 2923, 2945, 3179
LweI GCATC 5 cut(s) 375, 1173, 1195, 1264, 1710
MaeI CTAG 6 cut(s) 708, 1139, 1676, 2082, 2255, 2738
MaeII ACGT 3 cut(s) 7, 2549, 2914
MaeIII GTNAC 7 cut(s) 142, 265, 1025, 1087, 2359, 2783, 2821
MfeI CAATTG 1 cut(s) 2214
MflI RGATCY 3 cut(s) 55, 259, 2703
MhlI GDGCHC 2 cut(s) 2307, 3234
MlyI GAGTC 2 cut(s) 1418, 1797
MmeI TCCRAC 2 cut(s) 274, 3053
Mph1103I ATGCAT 2 cut(s) 1188, 2949
MroI TCCGGA 2 cut(s) 1799, 2299
MroXI GAANNNNTTC 3 cut(s) 783, 1423, 1631
MslI CAYNNNNRTG 2 cut(s) 1612, 1770
MspA1I CMGCKG 2 cut(s) 1655, 2936
MspCI CTTAAG 2 cut(s) 2125, 2699
MspI CCGG 4 cut(s) 684, 1800, 2300, 3134
MspR9I CCNGG 6 cut(s) 684, 1245, 1740, 2560, 2576, 3135
MunI CAATTG 1 cut(s) 2214
Mva1269I GAATGC 2 cut(s) 669, 1526
MvaI CCWGG 4 cut(s) 1245, 1740, 2560, 2576
MvnI CGCG 2 cut(s) 669, 2618
MwoI GCNNNNNNNGC 6 cut(s) 167, 287, 410, 2293, 2302, 3255
NciI CCSGG 2 cut(s) 684, 3135
NcoI CCATGG 1 cut(s) 2030
NdeI CATATG 1 cut(s) 286
NlaIV GGNNCC 9 cut(s) 57, 261, 512, 759, 966, 1634, 2569, 2764, 3077
NmeAIII GCCGAG 1 cut(s) 1429
NmuCI GTSAC 3 cut(s) 142, 1025, 1087
NsiI ATGCAT 2 cut(s) 1188, 2949
NspI RCATGY 7 cut(s) 328, 806, 1056, 1360, 1403, 1948, 3172
PaeI GCATGC 2 cut(s) 806, 1403
PagI TCATGA 2 cut(s) 1920, 1960
PciI ACATGT 1 cut(s) 324
PciSI GCTCTTC 1 cut(s) 532
PctI GAATGC 2 cut(s) 669, 1526
PdmI GAANNNNTTC 3 cut(s) 783, 1423, 1631
PflFI GACNNNGTC 1 cut(s) 1434
PflMI CCANNNNNTGG 2 cut(s) 898, 3035
PfoI TCCNGGA 3 cut(s) 682, 1738, 2558
PkrI GCNGC 8 cut(s) 172, 175, 412, 599, 2087, 2935, 2938, 3194
Ple19I CGATCG 2 cut(s) 307, 1024
PleI GAGTC 2 cut(s) 1417, 1797
PpsI GAGTC 2 cut(s) 1417, 1797
Ppu21I YACGTR 1 cut(s) 2915
PpuMI RGGWCCY 1 cut(s) 2567
PscI ACATGT 1 cut(s) 324
PshBI ATTAAT 2 cut(s) 1193, 1823
PsiI TTATAA 1 cut(s) 417
Psp124BI GAGCTC 1 cut(s) 2307
Psp5II RGGWCCY 1 cut(s) 2567
Psp6I CCWGG 4 cut(s) 1243, 1738, 2558, 2574
PspEI GGTNACC 1 cut(s) 142
PspGI CCWGG 4 cut(s) 1243, 1738, 2558, 2574
PspN4I GGNNCC 9 cut(s) 57, 261, 512, 759, 966, 1634, 2569, 2764, 3077
PspPI GGNCC 4 cut(s) 640, 757, 2012, 2567
PspPPI RGGWCCY 1 cut(s) 2567
PsrI GAACNNNNNNTAC 2 cut(s) 600, 632
PstNI CAGNNNCTG 1 cut(s) 3192
PsuI RGATCY 3 cut(s) 55, 259, 2703
PsyI GACNNNGTC 1 cut(s) 1434
PvuI CGATCG 2 cut(s) 307, 1024
PvuII CAGCTG 2 cut(s) 1655, 2936
RseI CAYNNNNRTG 2 cut(s) 1612, 1770
SacI GAGCTC 1 cut(s) 2307
SapI GCTCTTC 1 cut(s) 532
SatI GCNGC 8 cut(s) 171, 174, 411, 598, 2086, 2934, 2937, 3193
Sau96I GGNCC 4 cut(s) 640, 757, 2012, 2567
ScaI AGTACT 2 cut(s) 609, 1095
SchI GAGTC 2 cut(s) 1418, 1797
ScrFI CCNGG 6 cut(s) 684, 1245, 1740, 2560, 2576, 3135
SduI GDGCHC 2 cut(s) 2307, 3234
SfaNI GCATC 5 cut(s) 375, 1173, 1195, 1264, 1710
SfcI CTRYAG 3 cut(s) 474, 1588, 2644
SinI GGWCC 4 cut(s) 640, 757, 2012, 2567
SmiMI CAYNNNNRTG 2 cut(s) 1612, 1770
SmlI CTYRAG 5 cut(s) 1685, 1814, 2125, 2699, 2826
SmoI CTYRAG 5 cut(s) 1685, 1814, 2125, 2699, 2826
SphI GCATGC 2 cut(s) 806, 1403
SsiI CCGC 5 cut(s) 128, 174, 870, 2412, 2976
SspI AATATT 2 cut(s) 232, 1846
SspMI CTAG 6 cut(s) 708, 1139, 1676, 2082, 2255, 2738
SstI GAGCTC 1 cut(s) 2307
StyD4I CCNGG 6 cut(s) 682, 1243, 1738, 2558, 2574, 3133
StyI CCWWGG 1 cut(s) 2030
TaaI ACNGT 6 cut(s) 882, 1087, 1093, 2002, 2496, 2969
TaiI ACGT 3 cut(s) 10, 2552, 2917
TaqI TCGA 6 cut(s) 828, 1499, 1851, 2529, 2672, 3311
TaqII GACCGA 1 cut(s) 2000
TatI WGTACW 5 cut(s) 607, 1093, 2342, 2637, 3303
TauI GCSGC 1 cut(s) 176
TscAI CASTG 5 cut(s) 872, 2007, 2470, 2928, 3097
TseFI GTSAC 3 cut(s) 142, 1025, 1087
TseI GCWGC 7 cut(s) 170, 410, 597, 2085, 2933, 2936, 3192
Tsp45I GTSAC 3 cut(s) 142, 1025, 1087
TspGWI ACGGA 2 cut(s) 1322, 2257
TspRI CASTG 5 cut(s) 872, 2007, 2470, 2928, 3097
Tth111I GACNNNGTC 1 cut(s) 1434
Van91I CCANNNNNTGG 2 cut(s) 898, 3035
Vha464I CTTAAG 2 cut(s) 2125, 2699
VpaK11BI GGWCC 4 cut(s) 640, 757, 2012, 2567
VspI ATTAAT 2 cut(s) 1193, 1823
XagI CCTNNNNNAGG 1 cut(s) 828
XapI RAATTY 4 cut(s) 978, 987, 2323, 2991
XceI RCATGY 7 cut(s) 328, 806, 1056, 1360, 1403, 1948, 3172
XmiI GTMKAC 3 cut(s) 925, 1153, 2552
XmnI GAANNNNTTC 3 cut(s) 783, 1423, 1631
XspI CTAG 6 cut(s) 708, 1139, 1676, 2082, 2255, 2738
ZrmI AGTACT 2 cut(s) 609, 1095
Zsp2I ATGCAT 2 cut(s) 1188, 2949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.