Rroxscaffold_5G00349860

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000654
Physical Location & Seq
Forward (+)
23187393 .. 23191788
4396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00349860.1

Sequence Viewer

Length: 3519 bp
ATGAGCACACGGTTGGATGCTTCTTCTTCTTCCTCGTCCTCCCCTTACCGGTGTACTTATCATGCATTCTTGAGTTTCAGAGGTGAGGACACACGTAAGGGATTTACTGATCATCTTTACAGGGCCTTGGAGCTCGCAGGAATCCACACGTTTAGAGACGATGATGAAATCGAGAGAGGAGCAAATATCGCAGCAGAGTTACAGAGAGCTATCCAAGAGTCGAGGGTATCTGTGGTTGTGTTGTCTAGAGACTATGCTTCCTCAAGGTGGTGCTTGGATGAACTTGCAAAGATCATGGAACGTAGGAGAAATGATGACGCACACGTGGTTATTCCAGTGTTTTATGATGTAGATCCTTCCCATGTCAGGAAGCAAATCGGGAGTTTTGCTGAAGCTTTTGTTAGACATGAAGAGCGCTTCAAGGAGGAGATGGGAAAGGTTGCCGAGTGGAGAAGAGCGCTTTCTGATATTGCTGATCTTGGTGGAATGGTTTTAGGACAACGGTATGAATCACAATTTATCCAAGATATTGTTGAAGAAATCAGAAGTAAATTGGATTCCAAAGTCTTAAACATTGCTCCGTATGCAATTGGAATAGACGATCGTGTGCGAGGTATAAACATGTGGTTAGAAGATGGGTCAAATGATGTTGGTGTGGCTGTGATCTATGGAATGGGTGGAATTGGCAAAACCACTATTGCCAAAGCTGCTTATAACTTGAATTTCCATAGATTTCAAGGTAGCAGCTTTCTTGCTGATGTTAGAGAAACTTCAGAACAGCTGAATGGTTTGGTTCGCTTGCAAAGAAATCTTCTTTCAGATCTCCAAAAGGGGAAAACAAAGAAAATATACAACATTGATGAAGGAATACGAAAGATAAAAGATGCTGTATGTTGCAAGAAAGTTCTTATTGTTCTTGATGATGTGAACCACTCAGACCAATTTAATGCAATTATTGGAATGCGCAGATGGTTTGTTCCAGGAAGTAAGATAATCTTAACAACCAGATACGAACATTTGTTAAAGGCTCACGAAGTTAACACAATGTTTAAGGTTCAAGAGTTGAATGAGCATGAATCACTTGAGCTCCTCAGTTGGCACGCCTTTGGACAAGCCCATCCTATTGAAGCTTACATTGATCTTTCAAAACCTGTTGTAAAACACTGTGGAGGGATTCCATTAGCTCTGCAAGTTCTGGGATCTTCTTTGTCTGGCAGAAGTATAGATATATGGCAAAGCGCATTACAGAAGCTATGTGAGATTCCAGATGGAGAAATTCAAAAAATTCTTAGAATCAGTTTTGACTCTCTGCAAGATGATCATGACAAAAACTTATTCCTTCATATAGCATGCTTCTTCAGAGGAAAGAATAAAGAGTTTTCAATTACAGTACTGGACAACCTTAAATTCTACACAGTGATTGGAATTCAAAATCTGGTTGATAGATGTCTAGTGAAAATTAATGAAGACAACAAGTTGATAGTGCATCATTTGCTTCGAGACATGGCAAGAGGAATTATTCGTGAAGAATCACCTGAGGACCCTGGGAAACGCAGTAGGGTGTGGCACAAAGATGCCTTCAATATTTTGAGTAAAAGAACTCAGGGTACTGGAACTGTTAAGGGCATCATGCTTGACCTTTCTATGTTGATGCAAGATGAGTCTTCCAAAACATTATTTAGTGGACGTAACAGCAAACGACGTCGTGTTGATGATTATGATGGAAATTGTTCAGGACGGCGTCGACTTGGTCTCTTCACCTGGCAATTATTTAGTTTTTCCTCAGCCAACACGACTCCAGCATCAGATGAGGTTGACCTCAAAATAGAGGCATTCAAAAAGATGCACAACCTTGAAGTTCTCCTACTCAATAATGTACATTTCAGTCAAGGCTTTGAAAATTTTCCCAAAAACTTGATATGGCTGTCTTGGCAAGGATTCTCTTTAAATTCGATACCAGCCAGTTTGTATTTGAAAACTCTAGTTGTTCTTGACTTGCGGAATAGCAGCCTGCAATGTGTTTGGAAGGGAACCAAGTTTCTTCCAAGTCTGAAAGTCCTTAATCTTAGTCATTCACATGGCCTTTCAGCAACCCCCGACATATCAGGACTCCCTAACCTTGAGAGATTGATTCTAAAAAATTGTAGAAATTTGATTGAGGTTGATGAATCGATTGGAGACTTGGAGAAACTTGTTTTCTTAAATCTAAAAGACTGCAAAAATCTGATGAAGCTTCCGACCAGAATCAAAATGTCAAGTTCTCTTCAAACACTTATTCTTTCTGGTTGCTCAAAGCTTGTGCTTCATTCTAATATGGTTGCAACAAATCAGTCACTCTTGACAGCTAGTGATATGAAGAAAGACGATTGTTTATCACCTAACTTGTGGAAATCACTCTTATCATGGGCAGTACCAAGCAGAAATATCATATCAACCAGTTTCTCAGTATCAAATTTACCACGTTCTTTACGAAGCTTAAGCCTAGCTGACTGCAACCTATTAGAGATTCCAGGTGATCTTAGTATCCTTTCCTCATTGAAGTATTTAGATCTATCTGGGAACCCAATTCAGAGACTCCCAGAAAACATAAGATCTCTTAGTAAGCTTGAGACACTTATGTTAGAGGACTGCACAGAACTCAGAACGCTTCCAGAGCTCCCACCAAGTCTCCAGAGTTTGCTTGCAAGTTCTTGTAAGTCATTGAAAAGAATAACAAATTTACCAAACCTGTTCAAATCATTGGATACAGATTTTTTGGATTGCAAGAAGTTAGTTGAAGTTGAAAGCTTGTTCACTATAAAACCATTGAGAACCACTGACATAGAAATGATCAAAGATATGGGACTGTTCAATTTTAAATCCGTTGGAAGCTACACTGAGGTTGAAATGATCAACTACATCACAAATACAACCAAGAAGGTCCCCCTCCAGGTGTTGTCTGAATGTGGCATATCTAGCATTTACCTAAACGGAAACGAGATTCCAGATTCGTTTCGCCACAGGAGAACGGGCAACTCTGTGTTGTCTATTATAGTACCTCCACATCTTAATCATAGAATTCGAGGCTTAAATGCATGTGTTGTATATGCACGACGCCCAGATATTGGTCGTTCTACTAAATATCAAAACTTGTTTAAAGTCAGCAATGAGACCAAGGGTCTCATGTGGACCTATTCCCCTATCACAATAGACGAAGTACGTGTTTCGGTTGAGTTTGGATCCGAGCTTGGACACAATCTAAATTTATGGACAAAGGAATTGGGAATCCAACTTGTGTATGAGCAGGAAAGTAGTGAAGATGGAAAGAAACACAATCTCCTTGCTGGAGATGTGTCAGTGTCATCCTCAAGTTACCAAATGTGGACGGGAAAATACTTTCTTTGTAATAACTTGAATCGTGCTCAGAAAACACAATTCAAGAGTAGCCAGGAAAACCCGTCTCATCTTGATTCTGACAAAACCAAGAGCCCCTTCCTTTTCTTATTTGAGCATGATGATGTTTCACAGGCTGATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1172

Amino Acids

132.73

Weight (kDa)

8.27

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 188 6.5e-55 TIR domain
TIR_2 PF13676 23 - 120 3.7e-18 TIR domain
NB-ARC PF00931 201 - 369 4.4e-20 NB-ARC domain
WHD_ROQ1 PF23282 440 - 511 5.6e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 821 - 939 4.9e-09 Leucine-rich repeat region
LRR_8 PF13855 821 - 877 6.9e-07 Leucine rich repeat
LRR_4 PF12799 845 - 887 7.8e-06 Leucine Rich repeats (2 copies)
C-JID PF20160 990 - 1097 9.6e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
AatII GACGTC 1 cut(s) 1705
Acc16I TGCGCA 1 cut(s) 965
AccB7I CCANNNNNTGG 1 cut(s) 3104
AccI GTMKAC 1 cut(s) 1744
AciI CCGC 1 cut(s) 1999
AclWI GGATC 4 cut(s) 347, 1207, 3213, 3226
AcuI CTGAAG 3 cut(s) 411, 756, 1342
AcvI CACGTG 1 cut(s) 325
AcyI GRCGYC 3 cut(s) 1702, 1741, 3094
AfaI GTAC 7 cut(s) 55, 1392, 1609, 1878, 2412, 3036, 3198
AfeI AGCGCT 2 cut(s) 416, 459
AfiI CCNNNNNNNGG 5 cut(s) 48, 267, 366, 2929, 3104
AflII CTTAAG 1 cut(s) 2476
AflIII ACRYGT 5 cut(s) 92, 147, 322, 621, 3199
AgeI ACCGGT 1 cut(s) 48
AhdI GACNNNNNGTC 1 cut(s) 3156
AjnI CCWGG 6 cut(s) 979, 1543, 1760, 2509, 2928, 3426
AjuI GAANNNNNNNTTGG 2 cut(s) 3455, 3487
Alw21I GWGCWC 5 cut(s) 8, 135, 1089, 2658, 3403
AlwI GGATC 4 cut(s) 347, 1207, 3213, 3226
Aor51HI AGCGCT 2 cut(s) 416, 459
AoxI GGCC 2 cut(s) 123, 2080
ApeKI GCWGC 4 cut(s) 191, 707, 744, 2007
AseI ATTAAT 1 cut(s) 1461
AsiGI ACCGGT 1 cut(s) 48
Asp700I GAANNNNTTC 2 cut(s) 1729, 1902
AspLEI GCGC 4 cut(s) 417, 460, 966, 1241
AspS9I GGNCC 4 cut(s) 123, 1540, 2920, 3168
AsuHPI GGTGA 5 cut(s) 95, 1524, 1750, 2367, 2525
AvaII GGWCC 3 cut(s) 1540, 2920, 3168
AxyI CCTNAGG 1 cut(s) 1536
BamHI GGATCC 1 cut(s) 3218
BanII GRGCYC 4 cut(s) 135, 1089, 2658, 3470
BarI GAAGNNNNNNTAC 2 cut(s) 1848, 1880
BbrPI CACGTG 1 cut(s) 325
BbsI GAAGAC 2 cut(s) 1473, 1656
Bbv12I GWGCWC 5 cut(s) 8, 135, 1089, 2658, 3403
BbvCI CCTCAGC 1 cut(s) 1783
BbvI GCAGC 4 cut(s) 203, 694, 756, 2019
BccI CCATC 7 cut(s) 424, 629, 963, 1125, 1262, 1715, 3293
BceAI ACGGC 1 cut(s) 1754
BciT130I CCWGG 6 cut(s) 981, 1545, 1762, 2511, 2930, 3428
BciVI GTATCC 2 cut(s) 2534, 2737
BclI TGATCA 5 cut(s) 109, 1318, 2829, 2889, 3511
BfaI CTAG 6 cut(s) 246, 1451, 1982, 2346, 2483, 2955
BfoI RGCGCY 2 cut(s) 418, 461
BfrI CTTAAG 1 cut(s) 2476
BfuI GTATCC 2 cut(s) 2534, 2737
BglII AGATCT 3 cut(s) 820, 2548, 2591
BisI GCNGC 4 cut(s) 192, 708, 745, 2008
BlsI GCNGC 4 cut(s) 193, 709, 746, 2009
BmcAI AGTACT 1 cut(s) 1392
Bme1390I CCNGG 6 cut(s) 981, 1545, 1762, 2511, 2930, 3428
Bme18I GGWCC 3 cut(s) 1540, 2920, 3168
BmeRI GACNNNNNGTC 1 cut(s) 3156
BmgT120I GGNCC 4 cut(s) 123, 1540, 2920, 3168
BmiI GGNNCC 5 cut(s) 1542, 2032, 2561, 2922, 3220
BmrFI CCNGG 6 cut(s) 981, 1545, 1762, 2511, 2930, 3428
BmsI GCATC 8 cut(s) 7, 874, 1495, 1564, 1635, 1641, 1811, 1833
BpiI GAAGAC 2 cut(s) 1473, 1656
BpmI CTGGAG 4 cut(s) 1782, 2654, 2912, 3345
Bpu10I CCTNAGC 1 cut(s) 1783
BpuEI CTTGAG 6 cut(s) 91, 247, 1103, 2141, 2627, 3331
Bsa29I ATCGAT 1 cut(s) 2171
BsaAI YACGTR 3 cut(s) 95, 325, 3200
BsaBI GATNNNNATC 1 cut(s) 351
BsaHI GRCGYC 3 cut(s) 1702, 1741, 3094
BsaI GGTCTC 3 cut(s) 1757, 3143, 3164
BsaJI CCNNGG 4 cut(s) 126, 1543, 1544, 3153
BsaWI WCCGGW 1 cut(s) 48
BsaXI ACNNNNNCTCC 6 cut(s) 1071, 1101, 2652, 2682, 3300, 3330
Bsc4I CCNNNNNNNGG 5 cut(s) 48, 267, 366, 2929, 3104
Bse118I RCCGGY 1 cut(s) 48
Bse1I ACTGG 5 cut(s) 335, 1398, 1615, 1962, 2436
Bse21I CCTNAGG 1 cut(s) 1536
Bse3DI GCAATG 3 cut(s) 573, 2021, 3151
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 6 cut(s) 981, 1545, 1762, 2511, 2930, 3428
BseCI ATCGAT 1 cut(s) 2171
BseDI CCNNGG 4 cut(s) 126, 1543, 1544, 3153
BseGI GGATG 4 cut(s) 22, 283, 1117, 3341
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 5 cut(s) 48, 267, 366, 2929, 3104
BseMI GCAATG 3 cut(s) 573, 2021, 3151
BseMII CTCAG 9 cut(s) 948, 1105, 1527, 1616, 1797, 2457, 2653, 2868, 3416
BseNI ACTGG 5 cut(s) 335, 1398, 1615, 1962, 2436
BseRI GAGGAG 3 cut(s) 192, 440, 1079
BseXI GCAGC 4 cut(s) 203, 694, 756, 2019
BsgI GTGCAG 1 cut(s) 2614
Bsh1285I CGRYCG 1 cut(s) 604
BshFI GGCC 2 cut(s) 125, 2082
BshTI ACCGGT 1 cut(s) 48
BshVI ATCGAT 1 cut(s) 2171
BsiEI CGRYCG 1 cut(s) 604
BsiHKAI GWGCWC 5 cut(s) 8, 135, 1089, 2658, 3403
BsiSI CCGG 1 cut(s) 49
BslFI GGGAC 2 cut(s) 2856, 2906
BslI CCNNNNNNNGG 5 cut(s) 48, 267, 366, 2929, 3104
BsmBI CGTCTC 2 cut(s) 150, 3444
BsmFI GGGAC 2 cut(s) 2856, 2906
BsmI GAATGC 3 cut(s) 65, 966, 1832
BsnI GGCC 2 cut(s) 125, 2082
Bso31I GGTCTC 3 cut(s) 1757, 3143, 3164
Bsp1286I GDGCHC 6 cut(s) 8, 135, 1089, 2658, 3403, 3470
Bsp1407I TGTACA 1 cut(s) 1876
BspACI CCGC 1 cut(s) 1999
BspANI GGCC 2 cut(s) 125, 2082
BspCNI CTCAG 9 cut(s) 947, 1104, 1528, 1615, 1796, 2456, 2652, 2869, 3415
BspDI ATCGAT 1 cut(s) 2171
BspHI TCATGA 1 cut(s) 1321
BspLI GGNNCC 5 cut(s) 1542, 2032, 2561, 2922, 3220
BspPI GGATC 4 cut(s) 347, 1207, 3213, 3226
BspQI GCTCTTC 2 cut(s) 405, 448
BspTI CTTAAG 1 cut(s) 2476
BspTNI GGTCTC 3 cut(s) 1757, 3143, 3164
BsrDI GCAATG 3 cut(s) 573, 2021, 3151
BsrFI RCCGGY 1 cut(s) 48
BsrGI TGTACA 1 cut(s) 1876
BsrI ACTGG 5 cut(s) 335, 1398, 1615, 1962, 2436
BssAI RCCGGY 1 cut(s) 48
BssECI CCNNGG 4 cut(s) 126, 1543, 1544, 3153
BssNI GRCGYC 3 cut(s) 1702, 1741, 3094
BssT1I CCWWGG 2 cut(s) 126, 3153
Bst2UI CCWGG 6 cut(s) 981, 1545, 1762, 2511, 2930, 3428
Bst4CI ACNGT 7 cut(s) 12, 504, 1166, 1390, 1417, 1618, 2847
Bst6I CTCTTC 4 cut(s) 405, 448, 1760, 2268
BstACI GRCGYC 3 cut(s) 1702, 1741, 3094
BstAFI CTTAAG 1 cut(s) 2476
BstAPI GCANNNNNTGC 1 cut(s) 1492
BstAUI TGTACA 1 cut(s) 1876
BstBAI YACGTR 3 cut(s) 95, 325, 3200
BstC8I GCNNGC 6 cut(s) 135, 800, 1101, 1351, 2012, 2682
BstF5I GGATG 4 cut(s) 22, 283, 1117, 3341
BstH2I RGCGCY 2 cut(s) 418, 461
BstHHI GCGC 4 cut(s) 417, 460, 966, 1241
BstMCI CGRYCG 1 cut(s) 604
BstMWI GCNNNNNNNGC 7 cut(s) 188, 584, 707, 1492, 1930, 2653, 2955
BstNI CCWGG 6 cut(s) 981, 1545, 1762, 2511, 2930, 3428
BstNSI RCATGY 3 cut(s) 625, 1353, 3078
BstSCI CCNGG 6 cut(s) 979, 1543, 1760, 2509, 2928, 3426
BstV1I GCAGC 4 cut(s) 203, 694, 756, 2019
BstV2I GAAGAC 2 cut(s) 1473, 1656
BstX2I RGATCY 6 cut(s) 352, 820, 1199, 2548, 2591, 3218
BstYI RGATCY 6 cut(s) 352, 820, 1199, 2548, 2591, 3218
Bsu15I ATCGAT 1 cut(s) 2171
Bsu36I CCTNAGG 1 cut(s) 1536
BsuI GTATCC 2 cut(s) 2534, 2737
BsuRI GGCC 2 cut(s) 125, 2082
BsuTUI ATCGAT 1 cut(s) 2171
BtsCI GGATG 4 cut(s) 22, 283, 1117, 3341
BtsIMutI CAGTG 6 cut(s) 342, 1162, 1422, 2814, 2874, 3342
Cac8I GCNNGC 6 cut(s) 135, 800, 1101, 1351, 2012, 2682
CciI TCATGA 1 cut(s) 1321
CfoI GCGC 4 cut(s) 417, 460, 966, 1241
Cfr10I RCCGGY 1 cut(s) 48
Cfr13I GGNCC 4 cut(s) 123, 1540, 2920, 3168
ClaI ATCGAT 1 cut(s) 2171
CseI GACGC 3 cut(s) 326, 1730, 3102
Csp6I GTAC 7 cut(s) 54, 1391, 1608, 1877, 2411, 3035, 3197
CspAI ACCGGT 1 cut(s) 48
CviQI GTAC 7 cut(s) 54, 1391, 1608, 1877, 2411, 3035, 3197
DraI TTTAAA 3 cut(s) 1947, 2857, 3136
DriI GACNNNNNGTC 1 cut(s) 3156
Eam1104I CTCTTC 4 cut(s) 405, 448, 1760, 2268
Eam1105I GACNNNNNGTC 1 cut(s) 3156
EarI CTCTTC 4 cut(s) 405, 448, 1760, 2268
Ecl136II GAGCTC 3 cut(s) 133, 1087, 2656
Eco130I CCWWGG 2 cut(s) 126, 3153
Eco24I GRGCYC 4 cut(s) 135, 1089, 2658, 3470
Eco31I GGTCTC 3 cut(s) 1757, 3143, 3164
Eco47I GGWCC 3 cut(s) 1540, 2920, 3168
Eco47III AGCGCT 2 cut(s) 416, 459
Eco53kI GAGCTC 3 cut(s) 133, 1087, 2656
Eco57I CTGAAG 3 cut(s) 411, 756, 1342
Eco72I CACGTG 1 cut(s) 325
Eco81I CCTNAGG 1 cut(s) 1536
EcoICRI GAGCTC 3 cut(s) 133, 1087, 2656
EcoO109I RGGNCCY 3 cut(s) 123, 1540, 2920
EcoRI GAATTC 2 cut(s) 1425, 3057
EcoRII CCWGG 6 cut(s) 979, 1543, 1760, 2509, 2928, 3426
EcoT14I CCWWGG 2 cut(s) 126, 3153
EcoT22I ATGCAT 2 cut(s) 67, 3076
EcoT38I GRGCYC 4 cut(s) 135, 1089, 2658, 3470
ErhI CCWWGG 2 cut(s) 126, 3153
Esp3I CGTCTC 2 cut(s) 150, 3444
FalI AAGNNNNNCTT 4 cut(s) 980, 1012, 3455, 3487
FaqI GGGAC 2 cut(s) 2856, 2906
FbaI TGATCA 5 cut(s) 109, 1318, 2829, 2889, 3511
FblI GTMKAC 1 cut(s) 1744
Fnu4HI GCNGC 4 cut(s) 192, 708, 745, 2008
FokI GGATG 4 cut(s) 29, 290, 1104, 3328
FriOI GRGCYC 4 cut(s) 135, 1089, 2658, 3470
Fsp4HI GCNGC 4 cut(s) 192, 708, 745, 2008
FspBI CTAG 6 cut(s) 246, 1451, 1982, 2346, 2483, 2955
FspI TGCGCA 1 cut(s) 965
GlaI GCGC 4 cut(s) 416, 459, 965, 1240
GluI GCNGC 4 cut(s) 192, 708, 745, 2008
GsuI CTGGAG 4 cut(s) 1782, 2654, 2912, 3345
HaeII RGCGCY 2 cut(s) 418, 461
HaeIII GGCC 2 cut(s) 125, 2082
HapII CCGG 1 cut(s) 49
HgaI GACGC 3 cut(s) 326, 1730, 3102
HhaI GCGC 4 cut(s) 417, 460, 966, 1241
Hin1I GRCGYC 3 cut(s) 1702, 1741, 3094
Hin6I GCGC 4 cut(s) 415, 458, 964, 1239
HinP1I GCGC 4 cut(s) 415, 458, 964, 1239
HincII GTYRAC 3 cut(s) 1039, 1745, 1816
HindII GTYRAC 3 cut(s) 1039, 1745, 1816
HindIII AAGCTT 7 cut(s) 393, 1128, 2231, 2294, 2473, 2603, 2785
HpaI GTTAAC 1 cut(s) 1039
HpaII CCGG 1 cut(s) 49
HphI GGTGA 5 cut(s) 95, 1524, 1750, 2367, 2525
Hpy166II GTNNAC 9 cut(s) 54, 928, 1039, 1685, 1745, 1816, 2793, 3168, 3363
Hpy8I GTNNAC 9 cut(s) 54, 928, 1039, 1685, 1745, 1816, 2793, 3168, 3363
Hpy99I CGWCG 4 cut(s) 1704, 1707, 1746, 3096
HpyAV CCTTC 7 cut(s) 366, 857, 1349, 1588, 2020, 2911, 3481
HpyCH4III ACNGT 7 cut(s) 12, 504, 1166, 1390, 1417, 1618, 2847
HpyCH4IV ACGT 8 cut(s) 94, 149, 301, 324, 1687, 1702, 2461, 3199
HpyF10VI GCNNNNNNNGC 7 cut(s) 188, 584, 707, 1492, 1930, 2653, 2955
HpySE526I ACGT 8 cut(s) 94, 149, 301, 324, 1687, 1702, 2461, 3199
Hsp92I GRCGYC 3 cut(s) 1702, 1741, 3094
HspAI GCGC 4 cut(s) 415, 458, 964, 1239
Ksp22I TGATCA 5 cut(s) 109, 1318, 2829, 2889, 3511
KspAI GTTAAC 1 cut(s) 1039
LguI GCTCTTC 2 cut(s) 405, 448
LmnI GCTCC 5 cut(s) 130, 179, 583, 1092, 2661
Lsp1109I GCAGC 4 cut(s) 203, 694, 756, 2019
LweI GCATC 8 cut(s) 7, 874, 1495, 1564, 1635, 1641, 1811, 1833
MaeI CTAG 6 cut(s) 246, 1451, 1982, 2346, 2483, 2955
MaeII ACGT 8 cut(s) 94, 149, 301, 324, 1687, 1702, 2461, 3199
MaeIII GTNAC 4 cut(s) 198, 1688, 2331, 3350
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 6 cut(s) 352, 820, 1199, 2548, 2591, 3218
MhlI GDGCHC 6 cut(s) 8, 135, 1089, 2658, 3403, 3470
MlyI GAGTC 6 cut(s) 227, 1298, 1670, 1789, 2103, 2568
MmeI TCCRAC 3 cut(s) 2261, 2845, 3292
Mph1103I ATGCAT 2 cut(s) 67, 3076
MroXI GAANNNNTTC 2 cut(s) 1729, 1902
MslI CAYNNNNRTG 4 cut(s) 1572, 2076, 2825, 3495
MspA1I CMGCKG 1 cut(s) 781
MspCI CTTAAG 1 cut(s) 2476
MspI CCGG 1 cut(s) 49
MspR9I CCNGG 6 cut(s) 981, 1545, 1762, 2511, 2930, 3428
MunI CAATTG 1 cut(s) 588
Mva1269I GAATGC 3 cut(s) 65, 966, 1832
MvaI CCWGG 6 cut(s) 981, 1545, 1762, 2511, 2930, 3428
MwoI GCNNNNNNNGC 7 cut(s) 188, 584, 707, 1492, 1930, 2653, 2955
NlaIV GGNNCC 5 cut(s) 1542, 2032, 2561, 2922, 3220
NmeAIII GCCGAG 1 cut(s) 469
NmuCI GTSAC 1 cut(s) 2331
NsbI TGCGCA 1 cut(s) 965
NsiI ATGCAT 2 cut(s) 67, 3076
NspI RCATGY 3 cut(s) 625, 1353, 3078
PaeI GCATGC 1 cut(s) 1353
PagI TCATGA 1 cut(s) 1321
PasI CCCWGGG 1 cut(s) 1544
PciI ACATGT 1 cut(s) 621
PciSI GCTCTTC 2 cut(s) 405, 448
PcsI WCGNNNNNNNCGW 1 cut(s) 2467
PctI GAATGC 3 cut(s) 65, 966, 1832
PdmI GAANNNNTTC 2 cut(s) 1729, 1902
PflFI GACNNNGTC 2 cut(s) 1740, 1749
PflMI CCANNNNNTGG 1 cut(s) 3104
PfoI TCCNGGA 1 cut(s) 979
PinAI ACCGGT 1 cut(s) 48
PkrI GCNGC 4 cut(s) 193, 709, 746, 2009
Ple19I CGATCG 1 cut(s) 604
PleI GAGTC 6 cut(s) 226, 1298, 1669, 1789, 2103, 2568
PmaCI CACGTG 1 cut(s) 325
PmlI CACGTG 1 cut(s) 325
PpsI GAGTC 6 cut(s) 226, 1298, 1669, 1789, 2103, 2568
Ppu21I YACGTR 3 cut(s) 95, 325, 3200
PpuMI RGGWCCY 2 cut(s) 1540, 2920
PscI ACATGT 1 cut(s) 621
PshBI ATTAAT 1 cut(s) 1461
PsiI TTATAA 1 cut(s) 714
Psp124BI GAGCTC 3 cut(s) 135, 1089, 2658
Psp5II RGGWCCY 2 cut(s) 1540, 2920
Psp6I CCWGG 6 cut(s) 979, 1543, 1760, 2509, 2928, 3426
PspCI CACGTG 1 cut(s) 325
PspGI CCWGG 6 cut(s) 979, 1543, 1760, 2509, 2928, 3426
PspN4I GGNNCC 5 cut(s) 1542, 2032, 2561, 2922, 3220
PspPI GGNCC 4 cut(s) 123, 1540, 2920, 3168
PspPPI RGGWCCY 2 cut(s) 1540, 2920
PsrI GAACNNNNNNTAC 2 cut(s) 1591, 1623
PsuI RGATCY 6 cut(s) 352, 820, 1199, 2548, 2591, 3218
PsyI GACNNNGTC 2 cut(s) 1740, 1749
PvuI CGATCG 1 cut(s) 604
PvuII CAGCTG 1 cut(s) 781
RsaI GTAC 7 cut(s) 55, 1392, 1609, 1878, 2412, 3036, 3198
RsaNI GTAC 7 cut(s) 54, 1391, 1608, 1877, 2411, 3035, 3197
RseI CAYNNNNRTG 4 cut(s) 1572, 2076, 2825, 3495
SacI GAGCTC 3 cut(s) 135, 1089, 2658
SalI GTCGAC 1 cut(s) 1743
SapI GCTCTTC 2 cut(s) 405, 448
SatI GCNGC 4 cut(s) 192, 708, 745, 2008
Sau96I GGNCC 4 cut(s) 123, 1540, 2920, 3168
ScaI AGTACT 1 cut(s) 1392
SchI GAGTC 6 cut(s) 227, 1298, 1670, 1789, 2103, 2568
ScrFI CCNGG 6 cut(s) 981, 1545, 1762, 2511, 2930, 3428
SduI GDGCHC 6 cut(s) 8, 135, 1089, 2658, 3403, 3470
SfaNI GCATC 8 cut(s) 7, 874, 1495, 1564, 1635, 1641, 1811, 1833
SinI GGWCC 3 cut(s) 1540, 2920, 3168
SmiMI CAYNNNNRTG 4 cut(s) 1572, 2076, 2825, 3495
SmlI CTYRAG 7 cut(s) 70, 262, 1082, 2120, 2476, 2606, 3346
SmoI CTYRAG 7 cut(s) 70, 262, 1082, 2120, 2476, 2606, 3346
SphI GCATGC 1 cut(s) 1353
SsiI CCGC 1 cut(s) 1999
SspI AATATT 1 cut(s) 1585
SspMI CTAG 6 cut(s) 246, 1451, 1982, 2346, 2483, 2955
SstI GAGCTC 3 cut(s) 135, 1089, 2658
StyD4I CCNGG 6 cut(s) 979, 1543, 1760, 2509, 2928, 3426
StyI CCWWGG 2 cut(s) 126, 3153
TaaI ACNGT 7 cut(s) 12, 504, 1166, 1390, 1417, 1618, 2847
TaiI ACGT 8 cut(s) 97, 152, 304, 327, 1690, 1705, 2464, 3202
TaqI TCGA 7 cut(s) 171, 221, 1498, 1744, 1952, 2171, 3061
TatI WGTACW 3 cut(s) 53, 1390, 1876
TscAI CASTG 6 cut(s) 342, 1169, 1422, 2821, 2881, 3342
TseFI GTSAC 1 cut(s) 2331
TseI GCWGC 4 cut(s) 191, 707, 744, 2007
Tsp45I GTSAC 1 cut(s) 2331
TspGWI ACGGA 3 cut(s) 570, 2851, 2985
TspRI CASTG 6 cut(s) 342, 1169, 1422, 2821, 2881, 3342
Tth111I GACNNNGTC 2 cut(s) 1740, 1749
Van91I CCANNNNNTGG 1 cut(s) 3104
Vha464I CTTAAG 1 cut(s) 2476
VpaK11BI GGWCC 3 cut(s) 1540, 2920, 3168
VspI ATTAAT 1 cut(s) 1461
XbaI TCTAGA 1 cut(s) 245
XceI RCATGY 3 cut(s) 625, 1353, 3078
XmiI GTMKAC 1 cut(s) 1744
XmnI GAANNNNTTC 2 cut(s) 1729, 1902
XspI CTAG 6 cut(s) 246, 1451, 1982, 2346, 2483, 2955
ZraI GACGTC 1 cut(s) 1703
ZrmI AGTACT 1 cut(s) 1392
Zsp2I ATGCAT 2 cut(s) 67, 3076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.