Prupe.2G101900_v2.0.a1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000654
Physical Location & Seq
Forward (+)
15783336 .. 15788158
4823 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G101900.1

Sequence Viewer

Length: 3654 bp
ATGGCTGCTGCATTAAACCCCATGCTGCATGATGCTTCTTCTTCCTCCTCTTACCGGTGTAGCTATCACTCATTCTTGAGTTTCAGAGGCGAAGATACACGCAAGGGCTTCACCGATCACCTTTATAGGGCTTTGGAGCTGGCAGGAATCCACACGTTTAGAGACGATGATGAAATCGAGAGAGGAGCAGATATCGCAGCAGAGTTGAACAAAGCAATAAATGAGTCGAAAGTATCGATAATCGTCTTCTCCCAAAACTATGCTTCCTCAAGGTGGTGCTTGGACGAACTGGTGAAGATAATGGAACGTAGAAAACATGATGATGGCCATATTGTTATGCCAGTTTTCTATCATGTGGATCCATCCCATGTCAGGAACCAGAGAGGGAGCTTTGCAGAAGCATTTTCTAGACATGAAGAGCGCTTCAAGGAGGAGATGAACAAGGTGGAAGAATGGAGAAGAGCGCTTAAAGATGCAGCAGATCTGGCAGGGATGGCTTTAAAAGATAGCTATGAGTCGCAATTTATCCAAGACATTGTTAAAGAGATTGGAAATAAGCTGGATCCGAAGGTATTGAATGTTGCTCCCTATGCCGTTGGAATAGATGATCGCGTGCAAGGCATAAACATGTGGTTAGAAGATGGATCAAATGCTGTTGGTGTAGCTGTGATCTATGGGATGGGAGGAATTGGAAAGACCACCATTGCCAAAGCTGCTTATAACCGCAACTTTGGAAGATTTCAAGGCAGCAGCTTTCTTGCAGATATTAGAGAAGCTGCAGAACAGCCCTATGGTTTTGTTCGCTTGCAAAGGAAACTTCTTTCGGATATCCAAAAGGGGAAAGCAAAGAAAATAGACAACATAGATGAAGGAATAATCAAGATCAAACATGCTGTATGTAACAAAAGACTTCTTATTGTTCTTGATGATGTGAACGACATGGATCAATTTAATGCAATTCTTGGAATGAGAGAATGGTTTTATCCAGGAAGTAAAATTATCATAACAACCAGACATGAACATTTGTTAAAAGCTCATGAAGGTTGCACAATGTTTGAGGTTGAAGAATTGAATGAGTATGAATCACTTGAGCTTTTCAGTTGGCATGCCTTTGGACAACCCCAGCCTATCGAAGGCTACATGGAACTTTCGAGACCTGCAGTAGAACATTGTGGAGGAATTCCATTAGCTCTTCAAGTTCTGGGATCTTCTTTATCTGGGAAAGAAGTAGACGTATGGCGTAGCGCATTACAGAAGTTATGTGAAATTCCTAATGTCAAGATTCAAAAAATTCTTAGAATAAGCTATGATTCTCTGCAAGATGATCATGACCAAAATATATTCCTCCATATAGCCTATTTCTTCATTGGAAAGGAGAAGGATTTTACAATTGCAATACTGGACAACCTTAATTTTTACACAAGGATTGGAATTCAAAATCTAGTTGACAGATGTCTAGTAAAAATTAACAATGAAGACAACAGGTTGAACATGCATCACTTACTTAGAGACATGGGGAGGGGAATTGTTCGCGAAGAATCACCTCAAGACCCGGGAAGACGTAGTAGAGTGTGGCATAATGATGCCTTTAATATTCTGAGAAAAATGACTGGTACAGAAATGATTAAGGGCCTCATGCTCAACCTTCCTAAGTTGATGCAAGATGAGTCTTGCAAGACATTGTTTAGTAGAAGTAACAAAAAACGATCTCATGTTGAAGATTATGATGGAAGTTTTTCAAGACGTCGTCGACTTGATTTCTTCTCTTGGAAATCCATTGCAAGTAACTTTTCCTCAACAAATTCAGCTCCTGCATCAAATGAGGTGGACTTCAAAACAGAGGCATTTAAAAGGATGAACAATCTTGAGCTTCTCCAGCTCTATAATGTAAAAACCAGTGGAGGTTTTGAAGATTTTCCAAAAAACTTGGCATGGTTGTCTTGGCGAGGATTTCCTTTGAAATCACTACCAGCCAATTTTTGTTTGGAAAATCTAGTTGTTCTTGACTTGAGGAATAGCAGCCTGCAACATGTTTGGAAGGGACACAGGTTTCTTCCAAGGCTGAAAACACTCAACCTCAGCCATTCACATAGCCTAACGACAACCCCTGACATGTCAGGGCTCCCTAAGCTTGAGAGATTAATTCTTAAAGATTGCATAAACTTGGTTGAGGTTAATGAGTCCATTGGAGACTTAGAGAACCTTGTTCACTTGAACCTAAGAGACTGCAAAAATCTGATGAAGCTTCCAACGAGTATTAGAAGGTTGGGATCGCTTCAGGACCTCATTCTTTCGGGTTGCTCAAAACTTGAGCTTCATTCCAACACAAATGCAACAAATCAGGTAGACTCCACAGTTGGTGCTATGAAGAAATTCAATCTGTTATCAACTAAGTTATGGCAATCAATCGAGTCGTGGATATTGCCAAGAAAAAATCTTGTATCTTTCTCATTGGCAAGTTTACCACATTCAATCGAAAGGCTAAGTCTTGCTCACTGCAATGTAGCAGAGATTCCTAGTGAACTTGGTGCCCTATCGTCATTGAAGCATTTGGATCTATCTGCAACCCCAATTCTGAACCTACCAGGAAACATGAAGGGTCTTATTATGCTCCAGACTCTGTTGGTAGAAGGTTGCGCAAAGCTCCAAGCGCTTCCAGAGCTCCCAGCCAGTTTAAACTCACTGGAAGCAGGTCATTGTACATCATTGAAAAAAGTAACAAATTTACCAAACATTTTCACATCAATGAGTAAAAATCTTTGGGATTGCAATGAATTAGTTGAAGTAGAAAGCTTGTTTGAAATGAAACCATTGAGAAATGTTGACATAGAAATGATTAAAAATTTGGGATTGTTCAATTTGGAATCCAACGAAACCTCTGAGGTCGAAATGATCAACTACTTGACAAATACAACTAAGAAGTGTCGCCTTCAGGGATTGAACGAATGCGGCATATTTAGCATCTTCCTCCATGGGAACAAGATTCCAGATTGGTTCAGCTACAAGAGCTTGTGTAACTCCGTGTTGTCGATTGTTGTACCTTCACATCCTAATCTCAAGATCCGAGGCTTAAACGCATGCATTTTATACGCACGACGCCCCGATCACGAGGATGGTCCACATATGTTCAGTGAACACTTTGTTAAAGTGAGCAATGAGACCAAGGGTCTTATGTGGACGTACTTCCCTGTGGCAATGGGCCTTCCCAGAGAAAACCAAGACATGTTATGGCTAAGCCACTGGGTTTTCAGGGACAATGAACTGGAAAGCGGGGACGAAATACGCGTTTCAGTCAAGTCCGGTTTGTGGGCAAAGGAGTTCGGGATCCAACTTGTGCATGAAGAAGAAAATAAGGGTGAGGATGCTGGATCAAAAAGTGAAGATATAATAACACTTCCTTGGAACCAGAATGTTGATGTGCCAGTCTCAGTTTCAGCATCAAAGTATGAGATGTGGAGGGGCAAATACTTTCTATGCAACCATAGGTATCGAACTCATCAAGCTCAATTCAGAAGGTGCCAGCAGAATCCAGCTTATGGTGATTTTTCATACAAGCCAGGGGAATCCTTTCATTTGAACCGTTCCTTGTTTCACCACGAGGTTGATATAGAAGAGAACCAAATGCAGTTTGTTAGAAAAGCATTGACTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1218

Amino Acids

138.91

Weight (kDa)

6.7

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 720
AatII GACGTC 1 cut(s) 1747
Acc16I TGCGCA 1 cut(s) 2638
Acc36I ACCTGC 2 cut(s) 1165, 2681
AccB1I GGYRCC 2 cut(s) 2528, 3516
AccB7I CCANNNNNTGG 1 cut(s) 3536
AccI GTMKAC 3 cut(s) 1230, 1750, 2346
AccII CGCG 3 cut(s) 612, 1533, 3285
AciI CCGC 3 cut(s) 724, 2949, 3270
AcoI YGGCCR 1 cut(s) 325
AcsI RAATTY 8 cut(s) 1179, 1266, 1290, 1431, 1801, 2372, 2722, 2842
AcuI CTGAAG 2 cut(s) 2261, 2915
AcyI GRCGYC 2 cut(s) 1744, 3097
AfaI GTAC 4 cut(s) 1615, 2701, 3039, 3182
AfeI AGCGCT 3 cut(s) 422, 465, 2652
AfiI CCNNNNNNNGG 7 cut(s) 54, 127, 273, 372, 1133, 3306, 3536
AflIII ACRYGT 6 cut(s) 153, 627, 2029, 2112, 3222, 3283
AgeI ACCGGT 1 cut(s) 54
AhdI GACNNNNNGTC 1 cut(s) 3165
AjnI CCWGG 3 cut(s) 985, 2584, 3556
Alw21I GWGCWC 1 cut(s) 2664
Alw26I GTCTC 7 cut(s) 156, 1147, 1503, 2184, 2217, 3152, 3430
AlwNI CAGNNNCTG 2 cut(s) 1811, 2620
Ama87I CYCGRG 1 cut(s) 1552
Aor51HI AGCGCT 3 cut(s) 422, 465, 2652
AoxI GGCC 3 cut(s) 325, 1630, 3199
ApoI RAATTY 8 cut(s) 1179, 1266, 1290, 1431, 1801, 2372, 2722, 2842
AseI ATTAAT 1 cut(s) 2141
AsiGI ACCGGT 1 cut(s) 54
Asp700I GAANNNNTTC 4 cut(s) 1735, 1914, 2372, 3567
AspLEI GCGC 5 cut(s) 423, 466, 1247, 2639, 2653
AspS9I GGNCC 4 cut(s) 1630, 2281, 3116, 3199
AsuC2I CCSGG 2 cut(s) 1553, 1554
AsuHPI GGTGA 7 cut(s) 103, 110, 304, 1533, 3368, 3551, 3584
AvaI CYCGRG 1 cut(s) 1552
AvaII GGWCC 2 cut(s) 2281, 3116
BaeGI GKGCMC 1 cut(s) 2533
BalI TGGCCA 1 cut(s) 327
BamHI GGATCC 3 cut(s) 358, 562, 3324
BanI GGYRCC 2 cut(s) 2528, 3516
BanII GRGCYC 2 cut(s) 2124, 2664
BauI CACGAG 2 cut(s) 3107, 3596
BbsI GAAGAC 3 cut(s) 238, 1482, 1564
Bbv12I GWGCWC 1 cut(s) 2664
BbvCI CCTCAGC 1 cut(s) 2078
BbvI GCAGC 8 cut(s) 12, 209, 488, 700, 759, 762, 763, 2031
BccI CCATC 7 cut(s) 317, 370, 487, 635, 673, 1721, 3107
BceAI ACGGC 1 cut(s) 578
BciT130I CCWGG 3 cut(s) 987, 2586, 3558
BclI TGATCA 2 cut(s) 1324, 2892
BcnI CCSGG 2 cut(s) 1553, 1554
BcoDI GTCTC 7 cut(s) 156, 1147, 1503, 2184, 2217, 3152, 3430
BfaI CTAG 5 cut(s) 408, 1442, 1457, 1994, 2517
BfmI CTRYAG 2 cut(s) 777, 1158
BfoI RGCGCY 3 cut(s) 424, 467, 2654
BfuAI ACCTGC 2 cut(s) 1165, 2681
BglII AGATCT 1 cut(s) 481
BlpI GCTNAGC 1 cut(s) 3233
Bme1390I CCNGG 5 cut(s) 987, 1553, 1554, 2586, 3558
Bme18I GGWCC 2 cut(s) 2281, 3116
BmeRI GACNNNNNGTC 1 cut(s) 3165
BmeT110I CYCGRG 1 cut(s) 1552
BmgT120I GGNCC 4 cut(s) 1630, 2281, 3116, 3199
BmiI GGNNCC 8 cut(s) 360, 377, 564, 2123, 2530, 3326, 3404, 3518
BmrFI CCNGG 5 cut(s) 987, 1553, 1554, 2586, 3558
BmrI ACTGGG 1 cut(s) 3250
BmsI GCATC 9 cut(s) 22, 463, 1504, 1573, 1647, 1823, 2970, 3352, 3446
BmuI ACTGGG 1 cut(s) 3250
BoxI GACNNNNGTC 1 cut(s) 1452
BpiI GAAGAC 3 cut(s) 238, 1482, 1564
BpmI CTGGAG 2 cut(s) 1859, 2597
Bpu10I CCTNAGC 2 cut(s) 2078, 2127
Bpu1102I GCTNAGC 1 cut(s) 3233
BpuEI CTTGAG 9 cut(s) 97, 253, 1109, 1530, 1886, 2029, 2153, 2330, 3041
BpuMI CCSGG 2 cut(s) 1553, 1554
Bsa29I ATCGAT 1 cut(s) 236
BsaHI GRCGYC 2 cut(s) 1744, 3097
BsaI GGTCTC 2 cut(s) 1147, 3152
BsaJI CCNNGG 7 cut(s) 1552, 2057, 2971, 3064, 3162, 3398, 3557
BsaWI WCCGGW 2 cut(s) 54, 3299
Bsc4I CCNNNNNNNGG 7 cut(s) 54, 127, 273, 372, 1133, 3306, 3536
Bse118I RCCGGY 1 cut(s) 54
Bse3DI GCAATG 6 cut(s) 702, 1776, 2506, 2776, 3160, 3201
BseBI CCWGG 3 cut(s) 987, 2586, 3558
BseCI ATCGAT 1 cut(s) 236
BseDI CCNNGG 7 cut(s) 1552, 2057, 2971, 3064, 3162, 3398, 3557
BseGI GGATG 7 cut(s) 362, 498, 684, 1860, 3046, 3118, 3367
BseLI CCNNNNNNNGG 7 cut(s) 54, 127, 273, 372, 1133, 3306, 3536
BseMI GCAATG 6 cut(s) 702, 1776, 2506, 2776, 3160, 3201
BseMII CTCAG 4 cut(s) 1589, 2092, 2871, 3441
BseRI GAGGAG 3 cut(s) 37, 198, 446
BseSI GKGCMC 1 cut(s) 2533
BseXI GCAGC 8 cut(s) 12, 209, 488, 700, 759, 762, 763, 2031
BseYI CCCAGC 2 cut(s) 1122, 2665
Bsh1236I CGCG 3 cut(s) 612, 1533, 3285
BshFI GGCC 3 cut(s) 327, 1632, 3201
BshNI GGYRCC 2 cut(s) 2528, 3516
BshTI ACCGGT 1 cut(s) 54
BshVI ATCGAT 1 cut(s) 236
BsiHKAI GWGCWC 1 cut(s) 2664
BsiHKCI CYCGRG 1 cut(s) 1552
BsiSI CCGG 3 cut(s) 55, 1553, 3300
BslFI GGGAC 3 cut(s) 2055, 3266, 3287
BslI CCNNNNNNNGG 7 cut(s) 54, 127, 273, 372, 1133, 3306, 3536
BsmAI GTCTC 7 cut(s) 156, 1147, 1503, 2184, 2217, 3152, 3430
BsmBI CGTCTC 1 cut(s) 156
BsmFI GGGAC 3 cut(s) 2055, 3266, 3287
BsmI GAATGC 1 cut(s) 2951
BsnI GGCC 3 cut(s) 327, 1632, 3201
Bso31I GGTCTC 2 cut(s) 1147, 3152
BsoBI CYCGRG 1 cut(s) 1552
Bsp1286I GDGCHC 3 cut(s) 2124, 2533, 2664
Bsp1407I TGTACA 1 cut(s) 2699
Bsp1720I GCTNAGC 1 cut(s) 3233
Bsp19I CCATGG 1 cut(s) 2971
Bsp68I TCGCGA 1 cut(s) 1533
BspACI CCGC 3 cut(s) 724, 2949, 3270
BspANI GGCC 3 cut(s) 327, 1632, 3201
BspCNI CTCAG 4 cut(s) 1590, 2091, 2872, 3440
BspDI ATCGAT 1 cut(s) 236
BspFNI CGCG 3 cut(s) 612, 1533, 3285
BspHI TCATGA 2 cut(s) 1036, 1327
BspLI GGNNCC 8 cut(s) 360, 377, 564, 2123, 2530, 3326, 3404, 3518
BspMAI CTGCAG 2 cut(s) 781, 1162
BspMI ACCTGC 2 cut(s) 1165, 2681
BspQI GCTCTTC 3 cut(s) 411, 454, 1197
BspT107I GGYRCC 2 cut(s) 2528, 3516
BspTNI GGTCTC 2 cut(s) 1147, 3152
BsrDI GCAATG 6 cut(s) 702, 1776, 2506, 2776, 3160, 3201
BsrFI RCCGGY 1 cut(s) 54
BsrGI TGTACA 1 cut(s) 2699
BssAI RCCGGY 1 cut(s) 54
BssECI CCNNGG 7 cut(s) 1552, 2057, 2971, 3064, 3162, 3398, 3557
BssNI GRCGYC 2 cut(s) 1744, 3097
BssSI CACGAG 2 cut(s) 3107, 3596
BssT1I CCWWGG 4 cut(s) 2057, 2971, 3162, 3398
Bst2BI CACGAG 2 cut(s) 3107, 3596
Bst2UI CCWGG 3 cut(s) 987, 2586, 3558
Bst4CI ACNGT 2 cut(s) 2356, 3581
Bst6I CTCTTC 4 cut(s) 411, 454, 1197, 3606
BstACI GRCGYC 2 cut(s) 1744, 3097
BstAUI TGTACA 1 cut(s) 2699
BstC8I GCNNGC 7 cut(s) 141, 614, 806, 1107, 2024, 3079, 3521
BstDSI CCRYGG 1 cut(s) 2971
BstENI CCTNNNNNAGG 1 cut(s) 1131
BstF5I GGATG 7 cut(s) 362, 498, 684, 1860, 3046, 3118, 3367
BstFNI CGCG 3 cut(s) 612, 1533, 3285
BstH2I RGCGCY 3 cut(s) 424, 467, 2654
BstHHI GCGC 5 cut(s) 423, 466, 1247, 2639, 2653
BstMAI GTCTC 7 cut(s) 156, 1147, 1503, 2184, 2217, 3152, 3430
BstNI CCWGG 3 cut(s) 987, 2586, 3558
BstNSI RCATGY 8 cut(s) 631, 893, 1109, 1495, 2033, 2116, 3081, 3226
BstPAI GACNNNNGTC 1 cut(s) 1452
BstSCI CCNGG 5 cut(s) 985, 1551, 1552, 2584, 3556
BstSFI CTRYAG 2 cut(s) 777, 1158
BstSLI GKGCMC 1 cut(s) 2533
BstUI CGCG 3 cut(s) 612, 1533, 3285
BstV1I GCAGC 8 cut(s) 12, 209, 488, 700, 759, 762, 763, 2031
BstV2I GAAGAC 3 cut(s) 238, 1482, 1564
BstX2I RGATCY 7 cut(s) 358, 481, 562, 1205, 2554, 3060, 3324
BstYI RGATCY 7 cut(s) 358, 481, 562, 1205, 2554, 3060, 3324
Bsu15I ATCGAT 1 cut(s) 236
BsuRI GGCC 3 cut(s) 327, 1632, 3201
BsuTUI ATCGAT 1 cut(s) 236
BtgI CCRYGG 1 cut(s) 2971
BtsCI GGATG 7 cut(s) 362, 498, 684, 1860, 3046, 3118, 3367
BtsI GCAGTG 1 cut(s) 2494
BtsIMutI CAGTG 5 cut(s) 1903, 2494, 2681, 3136, 3238
BtuMI TCGCGA 1 cut(s) 1533
BveI ACCTGC 2 cut(s) 1165, 2681
Cac8I GCNNGC 7 cut(s) 141, 614, 806, 1107, 2024, 3079, 3521
CaiI CAGNNNCTG 2 cut(s) 1811, 2620
CciI TCATGA 2 cut(s) 1036, 1327
CfoI GCGC 5 cut(s) 423, 466, 1247, 2639, 2653
Cfr10I RCCGGY 1 cut(s) 54
Cfr13I GGNCC 4 cut(s) 1630, 2281, 3116, 3199
Cfr9I CCCGGG 1 cut(s) 1552
ClaI ATCGAT 1 cut(s) 236
CseI GACGC 1 cut(s) 3105
Csp6I GTAC 4 cut(s) 1614, 2700, 3038, 3181
CspAI ACCGGT 1 cut(s) 54
CspCI CAANNNNNGTGG 2 cut(s) 1806, 1841
CviQI GTAC 4 cut(s) 1614, 2700, 3038, 3181
DraI TTTAAA 3 cut(s) 501, 1849, 2676
DriI GACNNNNNGTC 1 cut(s) 3165
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 4 cut(s) 411, 454, 1197, 3606
Eam1105I GACNNNNNGTC 1 cut(s) 3165
EarI CTCTTC 4 cut(s) 411, 454, 1197, 3606
Ecl136II GAGCTC 1 cut(s) 2662
Eco130I CCWWGG 4 cut(s) 2057, 2971, 3162, 3398
Eco24I GRGCYC 2 cut(s) 2124, 2664
Eco31I GGTCTC 2 cut(s) 1147, 3152
Eco32I GATATC 2 cut(s) 193, 829
Eco47I GGWCC 2 cut(s) 2281, 3116
Eco47III AGCGCT 3 cut(s) 422, 465, 2652
Eco53kI GAGCTC 1 cut(s) 2662
Eco57I CTGAAG 2 cut(s) 2261, 2915
Eco88I CYCGRG 1 cut(s) 1552
EcoICRI GAGCTC 1 cut(s) 2662
EcoNI CCTNNNNNAGG 1 cut(s) 1131
EcoO109I RGGNCCY 2 cut(s) 1630, 2281
EcoRI GAATTC 2 cut(s) 1179, 1431
EcoRII CCWGG 3 cut(s) 985, 2584, 3556
EcoRV GATATC 2 cut(s) 193, 829
EcoT14I CCWWGG 4 cut(s) 2057, 2971, 3162, 3398
EcoT22I ATGCAT 2 cut(s) 1497, 3083
EcoT38I GRGCYC 2 cut(s) 2124, 2664
ErhI CCWWGG 4 cut(s) 2057, 2971, 3162, 3398
Esp3I CGTCTC 1 cut(s) 156
FaqI GGGAC 3 cut(s) 2055, 3266, 3287
FauI CCCGC 1 cut(s) 3263
FauNDI CATATG 1 cut(s) 3123
FbaI TGATCA 2 cut(s) 1324, 2892
FblI GTMKAC 3 cut(s) 1230, 1750, 2346
FokI GGATG 7 cut(s) 349, 505, 691, 1867, 3033, 3125, 3374
FriOI GRGCYC 2 cut(s) 2124, 2664
FspBI CTAG 5 cut(s) 408, 1442, 1457, 1994, 2517
FspI TGCGCA 1 cut(s) 2638
GlaI GCGC 5 cut(s) 422, 465, 1246, 2638, 2652
GsaI CCCAGC 2 cut(s) 1126, 2669
GsuI CTGGAG 2 cut(s) 1859, 2597
HaeII RGCGCY 3 cut(s) 424, 467, 2654
HaeIII GGCC 3 cut(s) 327, 1632, 3201
HapII CCGG 3 cut(s) 55, 1553, 3300
HgaI GACGC 1 cut(s) 3105
HhaI GCGC 5 cut(s) 423, 466, 1247, 2639, 2653
Hin1I GRCGYC 2 cut(s) 1744, 3097
Hin6I GCGC 5 cut(s) 421, 464, 1245, 2637, 2651
HinP1I GCGC 5 cut(s) 421, 464, 1245, 2637, 2651
HincII GTYRAC 3 cut(s) 1447, 1751, 2824
HindII GTYRAC 3 cut(s) 1447, 1751, 2824
HindIII AAGCTT 3 cut(s) 2129, 2243, 2791
HpaII CCGG 3 cut(s) 55, 1553, 3300
HphI GGTGA 7 cut(s) 103, 110, 304, 1533, 3368, 3551, 3584
Hpy188I TCNGA 9 cut(s) 86, 567, 826, 1599, 2238, 2577, 2881, 3065, 3512
Hpy99I CGWCG 3 cut(s) 1749, 1752, 3099
HpyCH4III ACNGT 2 cut(s) 2356, 3581
HpyCH4IV ACGT 6 cut(s) 155, 307, 1233, 1561, 1744, 3179
HpySE526I ACGT 6 cut(s) 155, 307, 1233, 1561, 1744, 3179
Hsp92I GRCGYC 2 cut(s) 1744, 3097
HspAI GCGC 5 cut(s) 421, 464, 1245, 2637, 2651
Ksp22I TGATCA 2 cut(s) 1324, 2892
LguI GCTCTTC 3 cut(s) 411, 454, 1197
LmnI GCTCC 9 cut(s) 136, 185, 387, 589, 1813, 2127, 2616, 2649, 2667
Lsp1109I GCAGC 8 cut(s) 12, 209, 488, 700, 759, 762, 763, 2031
LweI GCATC 9 cut(s) 22, 463, 1504, 1573, 1647, 1823, 2970, 3352, 3446
MaeI CTAG 5 cut(s) 408, 1442, 1457, 1994, 2517
MaeII ACGT 6 cut(s) 155, 307, 1233, 1561, 1744, 3179
MaeIII GTNAC 5 cut(s) 899, 1694, 1784, 2716, 3014
MfeI CAATTG 1 cut(s) 1389
MflI RGATCY 7 cut(s) 358, 481, 562, 1205, 2554, 3060, 3324
MhlI GDGCHC 3 cut(s) 2124, 2533, 2664
MlsI TGGCCA 1 cut(s) 327
MluI ACGCGT 1 cut(s) 3283
MluNI TGGCCA 1 cut(s) 327
MlyI GAGTC 7 cut(s) 233, 524, 1676, 2189, 2342, 2420, 2611
MmeI TCCRAC 5 cut(s) 577, 2273, 2346, 2892, 3352
Mox20I TGGCCA 1 cut(s) 327
Mph1103I ATGCAT 2 cut(s) 1497, 3083
MroXI GAANNNNTTC 4 cut(s) 1735, 1914, 2372, 3567
MscI TGGCCA 1 cut(s) 327
MslI CAYNNNNRTG 7 cut(s) 321, 626, 1581, 2499, 2744, 2831, 3111
Msp20I TGGCCA 1 cut(s) 327
MspI CCGG 3 cut(s) 55, 1553, 3300
MspR9I CCNGG 5 cut(s) 987, 1553, 1554, 2586, 3558
MssI GTTTAAAC 1 cut(s) 2676
MunI CAATTG 1 cut(s) 1389
Mva1269I GAATGC 1 cut(s) 2951
MvaI CCWGG 3 cut(s) 987, 2586, 3558
MvnI CGCG 3 cut(s) 612, 1533, 3285
NciI CCSGG 2 cut(s) 1553, 1554
NcoI CCATGG 1 cut(s) 2971
NdeI CATATG 1 cut(s) 3123
NlaIV GGNNCC 8 cut(s) 360, 377, 564, 2123, 2530, 3326, 3404, 3518
NruI TCGCGA 1 cut(s) 1533
NsbI TGCGCA 1 cut(s) 2638
NsiI ATGCAT 2 cut(s) 1497, 3083
NspI RCATGY 8 cut(s) 631, 893, 1109, 1495, 2033, 2116, 3081, 3226
PaeI GCATGC 2 cut(s) 1109, 3081
PagI TCATGA 2 cut(s) 1036, 1327
PciI ACATGT 4 cut(s) 627, 2029, 2112, 3222
PciSI GCTCTTC 3 cut(s) 411, 454, 1197
PcsI WCGNNNNNNNCGW 2 cut(s) 233, 3282
PctI GAATGC 1 cut(s) 2951
PdmI GAANNNNTTC 4 cut(s) 1735, 1914, 2372, 3567
PflFI GACNNNGTC 1 cut(s) 1746
PflMI CCANNNNNTGG 1 cut(s) 3536
PfoI TCCNGGA 1 cut(s) 985
PinAI ACCGGT 1 cut(s) 54
PleI GAGTC 7 cut(s) 232, 523, 1675, 2188, 2342, 2419, 2611
PmeI GTTTAAAC 1 cut(s) 2676
PpsI GAGTC 7 cut(s) 232, 523, 1675, 2188, 2342, 2419, 2611
PpuMI RGGWCCY 1 cut(s) 2281
PscI ACATGT 4 cut(s) 627, 2029, 2112, 3222
PshAI GACNNNNGTC 1 cut(s) 1452
PshBI ATTAAT 1 cut(s) 2141
PsiI TTATAA 1 cut(s) 720
Psp124BI GAGCTC 1 cut(s) 2664
Psp5II RGGWCCY 1 cut(s) 2281
Psp6I CCWGG 3 cut(s) 985, 2584, 3556
PspFI CCCAGC 2 cut(s) 1122, 2665
PspGI CCWGG 3 cut(s) 985, 2584, 3556
PspN4I GGNNCC 8 cut(s) 360, 377, 564, 2123, 2530, 3326, 3404, 3518
PspPI GGNCC 4 cut(s) 1630, 2281, 3116, 3199
PspPPI RGGWCCY 1 cut(s) 2281
PstI CTGCAG 2 cut(s) 781, 1162
PstNI CAGNNNCTG 2 cut(s) 1811, 2620
PsuI RGATCY 7 cut(s) 358, 481, 562, 1205, 2554, 3060, 3324
PsyI GACNNNGTC 1 cut(s) 1746
RruI TCGCGA 1 cut(s) 1533
RsaI GTAC 4 cut(s) 1615, 2701, 3039, 3182
RsaNI GTAC 4 cut(s) 1614, 2700, 3038, 3181
RseI CAYNNNNRTG 7 cut(s) 321, 626, 1581, 2499, 2744, 2831, 3111
SacI GAGCTC 1 cut(s) 2664
SalI GTCGAC 1 cut(s) 1749
SapI GCTCTTC 3 cut(s) 411, 454, 1197
Sau96I GGNCC 4 cut(s) 1630, 2281, 3116, 3199
SchI GAGTC 7 cut(s) 233, 524, 1676, 2189, 2342, 2420, 2611
ScrFI CCNGG 5 cut(s) 987, 1553, 1554, 2586, 3558
SduI GDGCHC 3 cut(s) 2124, 2533, 2664
SfaNI GCATC 9 cut(s) 22, 463, 1504, 1573, 1647, 1823, 2970, 3352, 3446
SfcI CTRYAG 2 cut(s) 777, 1158
SinI GGWCC 2 cut(s) 2281, 3116
SmaI CCCGGG 1 cut(s) 1554
SmiMI CAYNNNNRTG 7 cut(s) 321, 626, 1581, 2499, 2744, 2831, 3111
SmlI CTYRAG 9 cut(s) 76, 268, 1088, 1545, 1865, 2008, 2132, 2309, 3056
SmoI CTYRAG 9 cut(s) 76, 268, 1088, 1545, 1865, 2008, 2132, 2309, 3056
SphI GCATGC 2 cut(s) 1109, 3081
SsiI CCGC 3 cut(s) 724, 2949, 3270
SspI AATATT 1 cut(s) 1594
SspMI CTAG 5 cut(s) 408, 1442, 1457, 1994, 2517
SstI GAGCTC 1 cut(s) 2664
StyD4I CCNGG 5 cut(s) 985, 1551, 1552, 2584, 3556
StyI CCWWGG 4 cut(s) 2057, 2971, 3162, 3398
TaaI ACNGT 2 cut(s) 2356, 3581
TaiI ACGT 6 cut(s) 158, 310, 1236, 1564, 1747, 3182
TatI WGTACW 1 cut(s) 2699
TauI GCSGC 1 cut(s) 2952
TscAI CASTG 5 cut(s) 1903, 2501, 2688, 3136, 3245
TspGWI ACGGA 1 cut(s) 3010
TspMI CCCGGG 1 cut(s) 1552
TspRI CASTG 5 cut(s) 1903, 2501, 2688, 3136, 3245
Tth111I GACNNNGTC 1 cut(s) 1746
Van91I CCANNNNNTGG 1 cut(s) 3536
VpaK11BI GGWCC 2 cut(s) 2281, 3116
VspI ATTAAT 1 cut(s) 2141
XagI CCTNNNNNAGG 1 cut(s) 1131
XapI RAATTY 8 cut(s) 1179, 1266, 1290, 1431, 1801, 2372, 2722, 2842
XbaI TCTAGA 1 cut(s) 407
XceI RCATGY 8 cut(s) 631, 893, 1109, 1495, 2033, 2116, 3081, 3226
XcmI CCANNNNNNNNNTGG 1 cut(s) 1981
XmaI CCCGGG 1 cut(s) 1552
XmiI GTMKAC 3 cut(s) 1230, 1750, 2346
XmnI GAANNNNTTC 4 cut(s) 1735, 1914, 2372, 3567
XspI CTAG 5 cut(s) 408, 1442, 1457, 1994, 2517
ZraI GACGTC 1 cut(s) 1745
Zsp2I ATGCAT 2 cut(s) 1497, 3083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.