Rmu_sc0000575.1_g000003

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000575.1
OG0000654
Physical Location & Seq
Forward (+)
17214 .. 21527
4314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000575.1_g000003.1.cds

Sequence Viewer

Length: 3591 bp
atgagcacacagttggatgcttcttcttcttcttcctcgtcctccccttaccggtgtacttatcatgcattcttgagtttcagaggtgaggacacacgtaagggatttaccgatcatctttacagggccttggagctcgcaggaatccacacgtttagagacgatgatgaaatcgagagaggagcaaatatcgcagcagagttacagagagctatccaagagtcgagggtatctgtggttgtgttgtctagagactatgcttcatcaaggtggtgcttggacgaacttgcaaagatcatggaacgtaggagaaatgatgacgcacacgtggttattccagtgttttatgatgtagatccatcccatgtcaggaagcaaatcgggagttttgctcaagcttttgttagacatgaagagcgcttcaaggaggagatgggaaaggttgccgagtggagaagagcgctttctgatattgcagatcttggtggaatggttttaggaccacggtatgaatcacaatttatccaagatattgttgaagaaatcagaagtaaattggattccaaagtcttaaacattgctccgtatgcaattggaatagacgatcgtgttcgaggtataaacatgtggttagaagatgggtcaaatgatgttggtgtggctgtgatctgtggaatgggtggaattggcaaaaccactattgccaaagctgcttataacttgaatttccatagatttcaaggtagcagctttcttgctgatgttagagaaacttcagaacagccgaatggtttggttcgcttacaaagaaatcttctttcagatctccaaaaggggaaaacaaagaaaatatacaacattgatgaaggaatacgaaagataaaagatgctgtatgttgcaagaaagttcttattgttcttgatgatgtgaaccactcagaccaatttaatgcaattattggaatgcgcagatggtttgttccaggaagtaagataatcttaacaaccagatatgaacatttgttaaaggctcacgaagttaacacaatgtttaaggttcaagagttgaatgagcatgaatcacttgagctcctcagttggcacgcctttggacaaggccatcctattgaagcttacattgatctttcaaaacccgttgtaaaacattgtggagggattccattagctctgcaagttctgggatcttctttgtctggtagaagtatagatatatggcaaagcgcattacagaagctatgtgagattccagatggagaaattcaaaaaattcttagaatcagttttgactctctgcaagatgatcatgacaaaaacttattccttcatatagcatgcttcttcagaggaaagaataaagagttttcaattacagtactggacaaccttaaattctacacagtgattggaattcaaaatctggttgatagatgtctagtgaaaattaatgaagacaacaagttgatagtgcatcatttgcttcgagacatggcaaggggaattattctcgaagaatcacctgaggaccctgggaaacgcagtagggtgtggcacaaagatgccttcaatattttgagtaaaagaactggtactggaactgttaagggcatcatgcttgacctttctatgttgatgcaagataagtcttccaaaacattatttagtggaagtaacagcaaacgatgtcgtgttcatgattatgatggaaattgttcaggacggcgtcgacttggtctcttcacctggcaattatttagtttttcctcagccaacacgactccagcatcagatgaggttgacctcaaaatagaggcattcaaaaagatgcacaaccttgaagttctcctactcaataatgtacatttcagtcaaggctttgaaaattttccaaaaaacttgatatggctgtcttggcgaggattctctttaaattcgataccagccagtttgtatttgaaagctctagttgttcttgacttgcggaatagcagcctgcaatgtgtttggaagggaaccaagtctcttccaagtctgaaagtccttaatcttagtcattcacatggcctttcagcaacccccgacatatcaggactccctaaccttgagagattgattctaaaaaattgtagaaaattgattgaggttgatgaatcgattggagacttggagaaacttgttttcttaaatctaaaagactgcaaaaatctgatgaagcttccgaccagaatcaaaatgtcaagttctcttcaaacacttattctttctggttgctcaaagcttgtgcttcattctaatatggttgcaacaaatcagtcactcttgacagctagtgatatgaagaaagacaattgttcatcacctaacttgtggaaatcactcttattatgggcagtaccaagcagaaatatcttaccaaccagtttctcagtatcaaatttaccacgttctttacgaagcttaagcttagctgactgcaacctattagagattccaggtgatcttagtatcctctcctcattgaagtatttagatctatgtgggaacccaattcagagcctcccagaaaacataagatctcttagtaagcttgagacacttatgttagaggactgcacagaactcagaacgcttccagagctcccaccaagtctccagtgtttgcttgcaagttcttgtaaatcattgaaaagaataacaaatttaccaaacttgttcaaatcattggatacagattttctgcattgcaagaagttagttgaagttgaaagcttgttcactataaaaccattgacaaccactcacatagaaatgatcaaagatatgggactgttcaattttaaatccattgaaagcgacgctgaggttgaaatgatcaactacattacaaatacaaccaagaaggtccccctccaggtgttgtctgaatgtggcatatctagcatttacctaaacggaaacgagattccagattggtttcgccacaggagaatgggcaactctgtgttgtctattatagtacctccacatcttaatcatagaattcgaggcttaaatgcatgtgttgtatatgcacgacgcccagatgttggtcgttctgctaaatatcaaaacttgcttaaagtcagcaatgagaccaagggtctcatgtggacctattcccctatcacaataggtattccaaaggagaatgaagacatgttatggctaagccattggtttggagaacatgatctgggagggggagacgaagtacgtgtttcggttgagtttggacccgagcttggacacaatctaaatttatggacaaaggaattgggaatccaacttgtgtatgagcaggaaagtagtgaagatggaaagaaacacaatttccttgctggagatgtgtcagtgtcatcctcaagttaccaaatgtggacgggaaaatactttctttgtaataacctgaatcgtgctcagaaaacacaattccagagtagccaggaaaacccatctcatcttgattctgacaaaaccaagagccccttccttttcttatttgagcatgatgatgtttcacaggctgatcattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1196

Amino Acids

135.12

Weight (kDa)

7.74

Isoelectric Point (pI)

46.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 717
Acc16I TGCGCA 1 cut(s) 968
AccB7I CCANNNNNTGG 1 cut(s) 3104
AccI GTMKAC 1 cut(s) 1744
AciI CCGC 1 cut(s) 1999
AclWI GGATC 2 cut(s) 350, 1210
AcuI CTGAAG 2 cut(s) 759, 1345
AcvI CACGTG 1 cut(s) 328
AcyI GRCGYC 2 cut(s) 1741, 3094
AfaI GTAC 7 cut(s) 58, 1395, 1609, 1878, 2412, 3036, 3270
AfeI AGCGCT 2 cut(s) 419, 462
AfiI CCNNNNNNNGG 5 cut(s) 51, 369, 2929, 3104, 3299
AflII CTTAAG 1 cut(s) 2476
AflIII ACRYGT 6 cut(s) 95, 150, 325, 624, 3213, 3271
AgeI ACCGGT 1 cut(s) 51
AhdI GACNNNNNGTC 1 cut(s) 3156
AjnI CCWGG 6 cut(s) 982, 1546, 1760, 2509, 2928, 3498
AjuI GAANNNNNNNTTGG 2 cut(s) 3527, 3559
Alw21I GWGCWC 5 cut(s) 8, 138, 1092, 2658, 3475
AlwI GGATC 2 cut(s) 350, 1210
Ama87I CYCGRG 1 cut(s) 3293
Aor51HI AGCGCT 2 cut(s) 419, 462
AoxI GGCC 3 cut(s) 126, 1117, 2080
ApeKI GCWGC 4 cut(s) 194, 710, 747, 2007
AseI ATTAAT 1 cut(s) 1464
AsiGI ACCGGT 1 cut(s) 51
Asp700I GAANNNNTTC 2 cut(s) 1729, 1902
AspLEI GCGC 4 cut(s) 420, 463, 969, 1244
AspS9I GGNCC 6 cut(s) 126, 500, 1543, 2920, 3168, 3290
AsuHPI GGTGA 5 cut(s) 98, 1527, 1750, 2367, 2525
AvaI CYCGRG 1 cut(s) 3293
AvaII GGWCC 5 cut(s) 500, 1543, 2920, 3168, 3290
AxyI CCTNAGG 1 cut(s) 1539
BanII GRGCYC 4 cut(s) 138, 1092, 2658, 3542
BarI GAAGNNNNNNTAC 2 cut(s) 1848, 1880
BbrPI CACGTG 1 cut(s) 328
BbsI GAAGAC 3 cut(s) 1476, 1656, 3216
Bbv12I GWGCWC 5 cut(s) 8, 138, 1092, 2658, 3475
BbvCI CCTCAGC 2 cut(s) 1783, 2877
BbvI GCAGC 4 cut(s) 206, 697, 759, 2019
BccI CCATC 9 cut(s) 367, 427, 632, 966, 1128, 1265, 1715, 3365, 3517
BceAI ACGGC 1 cut(s) 1754
BciT130I CCWGG 6 cut(s) 984, 1548, 1762, 2511, 2930, 3500
BciVI GTATCC 2 cut(s) 2534, 2737
BclI TGATCA 4 cut(s) 1321, 2829, 2889, 3583
BfaI CTAG 5 cut(s) 249, 1454, 1982, 2346, 2955
BfoI RGCGCY 2 cut(s) 421, 464
BfrI CTTAAG 1 cut(s) 2476
BfuI GTATCC 2 cut(s) 2534, 2737
BglII AGATCT 4 cut(s) 478, 823, 2548, 2591
BisI GCNGC 4 cut(s) 195, 711, 748, 2008
BlpI GCTNAGC 2 cut(s) 2482, 3224
BlsI GCNGC 4 cut(s) 196, 712, 749, 2009
BmcAI AGTACT 1 cut(s) 1395
Bme1390I CCNGG 6 cut(s) 984, 1548, 1762, 2511, 2930, 3500
Bme18I GGWCC 5 cut(s) 500, 1543, 2920, 3168, 3290
BmeRI GACNNNNNGTC 1 cut(s) 3156
BmeT110I CYCGRG 1 cut(s) 3293
BmgT120I GGNCC 6 cut(s) 126, 500, 1543, 2920, 3168, 3290
BmiI GGNNCC 5 cut(s) 1545, 2032, 2561, 2922, 3292
BmrFI CCNGG 6 cut(s) 984, 1548, 1762, 2511, 2930, 3500
BmsI GCATC 8 cut(s) 7, 877, 1498, 1567, 1635, 1641, 1811, 1833
BpiI GAAGAC 3 cut(s) 1476, 1656, 3216
BplI GAGNNNNNCTC 2 cut(s) 376, 408
BpmI CTGGAG 4 cut(s) 1782, 2654, 2912, 3417
Bpu10I CCTNAGC 2 cut(s) 1783, 2877
Bpu1102I GCTNAGC 2 cut(s) 2482, 3224
BpuEI CTTGAG 6 cut(s) 94, 378, 1106, 2141, 2627, 3403
Bsa29I ATCGAT 1 cut(s) 2171
BsaAI YACGTR 3 cut(s) 98, 328, 3272
BsaBI GATNNNNATC 1 cut(s) 354
BsaHI GRCGYC 2 cut(s) 1741, 3094
BsaI GGTCTC 3 cut(s) 1757, 3143, 3164
BsaJI CCNNGG 5 cut(s) 129, 503, 1546, 1547, 3153
BsaWI WCCGGW 1 cut(s) 51
BsaXI ACNNNNNCTCC 6 cut(s) 1074, 1104, 2652, 2682, 3252, 3282
Bsc4I CCNNNNNNNGG 5 cut(s) 51, 369, 2929, 3104, 3299
Bse118I RCCGGY 1 cut(s) 51
Bse1I ACTGG 7 cut(s) 338, 1401, 1609, 1615, 1962, 2436, 2671
Bse21I CCTNAGG 1 cut(s) 1539
Bse3DI GCAATG 4 cut(s) 576, 2021, 2758, 3151
Bse8I GATNNNNATC 1 cut(s) 354
BseBI CCWGG 6 cut(s) 984, 1548, 1762, 2511, 2930, 3500
BseCI ATCGAT 1 cut(s) 2171
BseDI CCNNGG 5 cut(s) 129, 503, 1546, 1547, 3153
BseGI GGATG 4 cut(s) 22, 359, 1120, 3413
BseJI GATNNNNATC 1 cut(s) 354
BseLI CCNNNNNNNGG 5 cut(s) 51, 369, 2929, 3104, 3299
BseMI GCAATG 4 cut(s) 576, 2021, 2758, 3151
BseMII CTCAG 8 cut(s) 951, 1108, 1530, 1797, 2457, 2653, 2868, 3488
BseNI ACTGG 7 cut(s) 338, 1401, 1609, 1615, 1962, 2436, 2671
BseRI GAGGAG 4 cut(s) 195, 443, 1082, 2521
BseXI GCAGC 4 cut(s) 206, 697, 759, 2019
BsgI GTGCAG 1 cut(s) 2614
Bsh1285I CGRYCG 1 cut(s) 607
BshFI GGCC 3 cut(s) 128, 1119, 2082
BshTI ACCGGT 1 cut(s) 51
BshVI ATCGAT 1 cut(s) 2171
BsiEI CGRYCG 1 cut(s) 607
BsiHKAI GWGCWC 5 cut(s) 8, 138, 1092, 2658, 3475
BsiHKCI CYCGRG 1 cut(s) 3293
BsiSI CCGG 1 cut(s) 52
BslFI GGGAC 2 cut(s) 2856, 2906
BslI CCNNNNNNNGG 5 cut(s) 51, 369, 2929, 3104, 3299
BsmBI CGTCTC 2 cut(s) 153, 3255
BsmFI GGGAC 2 cut(s) 2856, 2906
BsmI GAATGC 3 cut(s) 68, 969, 1832
BsnI GGCC 3 cut(s) 128, 1119, 2082
Bso31I GGTCTC 3 cut(s) 1757, 3143, 3164
BsoBI CYCGRG 1 cut(s) 3293
Bsp1286I GDGCHC 6 cut(s) 8, 138, 1092, 2658, 3475, 3542
Bsp1407I TGTACA 1 cut(s) 1876
Bsp1720I GCTNAGC 2 cut(s) 2482, 3224
BspACI CCGC 1 cut(s) 1999
BspANI GGCC 3 cut(s) 128, 1119, 2082
BspCNI CTCAG 8 cut(s) 950, 1107, 1531, 1796, 2456, 2652, 2869, 3487
BspDI ATCGAT 1 cut(s) 2171
BspHI TCATGA 2 cut(s) 1324, 1711
BspLI GGNNCC 5 cut(s) 1545, 2032, 2561, 2922, 3292
BspPI GGATC 2 cut(s) 350, 1210
BspQI GCTCTTC 2 cut(s) 408, 451
BspTI CTTAAG 1 cut(s) 2476
BspTNI GGTCTC 3 cut(s) 1757, 3143, 3164
BsrDI GCAATG 4 cut(s) 576, 2021, 2758, 3151
BsrFI RCCGGY 1 cut(s) 51
BsrGI TGTACA 1 cut(s) 1876
BsrI ACTGG 7 cut(s) 338, 1401, 1609, 1615, 1962, 2436, 2671
BssAI RCCGGY 1 cut(s) 51
BssECI CCNNGG 5 cut(s) 129, 503, 1546, 1547, 3153
BssNI GRCGYC 2 cut(s) 1741, 3094
BssT1I CCWWGG 2 cut(s) 129, 3153
Bst2UI CCWGG 6 cut(s) 984, 1548, 1762, 2511, 2930, 3500
Bst4CI ACNGT 6 cut(s) 12, 507, 1393, 1420, 1618, 2847
Bst6I CTCTTC 5 cut(s) 408, 451, 1760, 2046, 2268
BstACI GRCGYC 2 cut(s) 1741, 3094
BstAFI CTTAAG 1 cut(s) 2476
BstAPI GCANNNNNTGC 1 cut(s) 1495
BstAUI TGTACA 1 cut(s) 1876
BstBAI YACGTR 3 cut(s) 98, 328, 3272
BstC8I GCNNGC 5 cut(s) 138, 1104, 1354, 2012, 2682
BstDSI CCRYGG 1 cut(s) 503
BstF5I GGATG 4 cut(s) 22, 359, 1120, 3413
BstH2I RGCGCY 2 cut(s) 421, 464
BstHHI GCGC 4 cut(s) 420, 463, 969, 1244
BstMCI CGRYCG 1 cut(s) 607
BstMWI GCNNNNNNNGC 7 cut(s) 191, 587, 710, 1495, 1930, 2653, 2955
BstNI CCWGG 6 cut(s) 984, 1548, 1762, 2511, 2930, 3500
BstNSI RCATGY 4 cut(s) 628, 1356, 3078, 3217
BstSCI CCNGG 6 cut(s) 982, 1546, 1760, 2509, 2928, 3498
BstV1I GCAGC 4 cut(s) 206, 697, 759, 2019
BstV2I GAAGAC 3 cut(s) 1476, 1656, 3216
BstX2I RGATCY 6 cut(s) 355, 478, 823, 1202, 2548, 2591
BstXI CCANNNNNNTGG 1 cut(s) 3236
BstYI RGATCY 6 cut(s) 355, 478, 823, 1202, 2548, 2591
Bsu15I ATCGAT 1 cut(s) 2171
Bsu36I CCTNAGG 1 cut(s) 1539
BsuI GTATCC 2 cut(s) 2534, 2737
BsuRI GGCC 3 cut(s) 128, 1119, 2082
BsuTUI ATCGAT 1 cut(s) 2171
BtgI CCRYGG 1 cut(s) 503
BtsCI GGATG 4 cut(s) 22, 359, 1120, 3413
BtsIMutI CAGTG 4 cut(s) 345, 1425, 2678, 3414
Cac8I GCNNGC 5 cut(s) 138, 1104, 1354, 2012, 2682
CciI TCATGA 2 cut(s) 1324, 1711
CfoI GCGC 4 cut(s) 420, 463, 969, 1244
Cfr10I RCCGGY 1 cut(s) 51
Cfr13I GGNCC 6 cut(s) 126, 500, 1543, 2920, 3168, 3290
ClaI ATCGAT 1 cut(s) 2171
CseI GACGC 4 cut(s) 329, 1730, 2882, 3102
Csp6I GTAC 7 cut(s) 57, 1394, 1608, 1877, 2411, 3035, 3269
CspAI ACCGGT 1 cut(s) 51
CviQI GTAC 7 cut(s) 57, 1394, 1608, 1877, 2411, 3035, 3269
DraI TTTAAA 2 cut(s) 1947, 2857
DriI GACNNNNNGTC 1 cut(s) 3156
Eam1104I CTCTTC 5 cut(s) 408, 451, 1760, 2046, 2268
Eam1105I GACNNNNNGTC 1 cut(s) 3156
EarI CTCTTC 5 cut(s) 408, 451, 1760, 2046, 2268
Ecl136II GAGCTC 3 cut(s) 136, 1090, 2656
Eco130I CCWWGG 2 cut(s) 129, 3153
Eco24I GRGCYC 4 cut(s) 138, 1092, 2658, 3542
Eco31I GGTCTC 3 cut(s) 1757, 3143, 3164
Eco47I GGWCC 5 cut(s) 500, 1543, 2920, 3168, 3290
Eco47III AGCGCT 2 cut(s) 419, 462
Eco53kI GAGCTC 3 cut(s) 136, 1090, 2656
Eco57I CTGAAG 2 cut(s) 759, 1345
Eco72I CACGTG 1 cut(s) 328
Eco81I CCTNAGG 1 cut(s) 1539
Eco88I CYCGRG 1 cut(s) 3293
EcoICRI GAGCTC 3 cut(s) 136, 1090, 2656
EcoO109I RGGNCCY 3 cut(s) 126, 1543, 2920
EcoRI GAATTC 2 cut(s) 1428, 3057
EcoRII CCWGG 6 cut(s) 982, 1546, 1760, 2509, 2928, 3498
EcoT14I CCWWGG 2 cut(s) 129, 3153
EcoT22I ATGCAT 2 cut(s) 70, 3076
EcoT38I GRGCYC 4 cut(s) 138, 1092, 2658, 3542
ErhI CCWWGG 2 cut(s) 129, 3153
Esp3I CGTCTC 2 cut(s) 153, 3255
FalI AAGNNNNNCTT 4 cut(s) 983, 1015, 3527, 3559
FaqI GGGAC 2 cut(s) 2856, 2906
FbaI TGATCA 4 cut(s) 1321, 2829, 2889, 3583
FblI GTMKAC 1 cut(s) 1744
Fnu4HI GCNGC 4 cut(s) 195, 711, 748, 2008
FokI GGATG 4 cut(s) 29, 346, 1107, 3400
FriOI GRGCYC 4 cut(s) 138, 1092, 2658, 3542
Fsp4HI GCNGC 4 cut(s) 195, 711, 748, 2008
FspBI CTAG 5 cut(s) 249, 1454, 1982, 2346, 2955
FspI TGCGCA 1 cut(s) 968
GlaI GCGC 4 cut(s) 419, 462, 968, 1243
GluI GCNGC 4 cut(s) 195, 711, 748, 2008
GsuI CTGGAG 4 cut(s) 1782, 2654, 2912, 3417
HaeII RGCGCY 2 cut(s) 421, 464
HaeIII GGCC 3 cut(s) 128, 1119, 2082
HapII CCGG 1 cut(s) 52
HgaI GACGC 4 cut(s) 329, 1730, 2882, 3102
HhaI GCGC 4 cut(s) 420, 463, 969, 1244
Hin1I GRCGYC 2 cut(s) 1741, 3094
Hin6I GCGC 4 cut(s) 418, 461, 967, 1242
HinP1I GCGC 4 cut(s) 418, 461, 967, 1242
HincII GTYRAC 3 cut(s) 1042, 1745, 1816
HindII GTYRAC 3 cut(s) 1042, 1745, 1816
HindIII AAGCTT 8 cut(s) 396, 1131, 2231, 2294, 2473, 2479, 2603, 2785
HpaI GTTAAC 1 cut(s) 1042
HpaII CCGG 1 cut(s) 52
HphI GGTGA 5 cut(s) 98, 1527, 1750, 2367, 2525
Hpy166II GTNNAC 8 cut(s) 57, 931, 1042, 1745, 1816, 2793, 3168, 3435
Hpy8I GTNNAC 8 cut(s) 57, 931, 1042, 1745, 1816, 2793, 3168, 3435
Hpy99I CGWCG 3 cut(s) 1746, 2876, 3096
HpyAV CCTTC 6 cut(s) 860, 1352, 1591, 2020, 2911, 3553
HpyCH4III ACNGT 6 cut(s) 12, 507, 1393, 1420, 1618, 2847
HpyCH4IV ACGT 6 cut(s) 97, 152, 304, 327, 2461, 3271
HpyF10VI GCNNNNNNNGC 7 cut(s) 191, 587, 710, 1495, 1930, 2653, 2955
HpySE526I ACGT 6 cut(s) 97, 152, 304, 327, 2461, 3271
Hsp92I GRCGYC 2 cut(s) 1741, 3094
HspAI GCGC 4 cut(s) 418, 461, 967, 1242
Ksp22I TGATCA 4 cut(s) 1321, 2829, 2889, 3583
KspAI GTTAAC 1 cut(s) 1042
LguI GCTCTTC 2 cut(s) 408, 451
LmnI GCTCC 5 cut(s) 133, 182, 586, 1095, 2661
Lsp1109I GCAGC 4 cut(s) 206, 697, 759, 2019
LweI GCATC 8 cut(s) 7, 877, 1498, 1567, 1635, 1641, 1811, 1833
MaeI CTAG 5 cut(s) 249, 1454, 1982, 2346, 2955
MaeII ACGT 6 cut(s) 97, 152, 304, 327, 2461, 3271
MaeIII GTNAC 4 cut(s) 201, 1688, 2331, 3422
MfeI CAATTG 2 cut(s) 591, 2365
MflI RGATCY 6 cut(s) 355, 478, 823, 1202, 2548, 2591
MhlI GDGCHC 6 cut(s) 8, 138, 1092, 2658, 3475, 3542
MlyI GAGTC 4 cut(s) 230, 1301, 1789, 2103
MmeI TCCRAC 2 cut(s) 2261, 3364
Mph1103I ATGCAT 2 cut(s) 70, 3076
MroXI GAANNNNTTC 2 cut(s) 1729, 1902
MslI CAYNNNNRTG 6 cut(s) 268, 1575, 1716, 2076, 2825, 3567
MspCI CTTAAG 1 cut(s) 2476
MspI CCGG 1 cut(s) 52
MspR9I CCNGG 6 cut(s) 984, 1548, 1762, 2511, 2930, 3500
MunI CAATTG 2 cut(s) 591, 2365
Mva1269I GAATGC 3 cut(s) 68, 969, 1832
MvaI CCWGG 6 cut(s) 984, 1548, 1762, 2511, 2930, 3500
MwoI GCNNNNNNNGC 7 cut(s) 191, 587, 710, 1495, 1930, 2653, 2955
NlaIV GGNNCC 5 cut(s) 1545, 2032, 2561, 2922, 3292
NmeAIII GCCGAG 1 cut(s) 472
NmuCI GTSAC 1 cut(s) 2331
NsbI TGCGCA 1 cut(s) 968
NsiI ATGCAT 2 cut(s) 70, 3076
NspI RCATGY 4 cut(s) 628, 1356, 3078, 3217
PaeI GCATGC 1 cut(s) 1356
PagI TCATGA 2 cut(s) 1324, 1711
PasI CCCWGGG 1 cut(s) 1547
PciI ACATGT 2 cut(s) 624, 3213
PciSI GCTCTTC 2 cut(s) 408, 451
PcsI WCGNNNNNNNCGW 1 cut(s) 2467
PctI GAATGC 3 cut(s) 68, 969, 1832
PdmI GAANNNNTTC 2 cut(s) 1729, 1902
PflFI GACNNNGTC 2 cut(s) 1740, 1749
PflMI CCANNNNNTGG 1 cut(s) 3104
PfoI TCCNGGA 1 cut(s) 982
PinAI ACCGGT 1 cut(s) 51
PkrI GCNGC 4 cut(s) 196, 712, 749, 2009
Ple19I CGATCG 1 cut(s) 607
PleI GAGTC 4 cut(s) 229, 1301, 1789, 2103
PmaCI CACGTG 1 cut(s) 328
PmlI CACGTG 1 cut(s) 328
PpsI GAGTC 4 cut(s) 229, 1301, 1789, 2103
Ppu21I YACGTR 3 cut(s) 98, 328, 3272
PpuMI RGGWCCY 2 cut(s) 1543, 2920
PscI ACATGT 2 cut(s) 624, 3213
PshBI ATTAAT 1 cut(s) 1464
PsiI TTATAA 1 cut(s) 717
Psp124BI GAGCTC 3 cut(s) 138, 1092, 2658
Psp5II RGGWCCY 2 cut(s) 1543, 2920
Psp6I CCWGG 6 cut(s) 982, 1546, 1760, 2509, 2928, 3498
PspCI CACGTG 1 cut(s) 328
PspGI CCWGG 6 cut(s) 982, 1546, 1760, 2509, 2928, 3498
PspN4I GGNNCC 5 cut(s) 1545, 2032, 2561, 2922, 3292
PspPI GGNCC 6 cut(s) 126, 500, 1543, 2920, 3168, 3290
PspPPI RGGWCCY 2 cut(s) 1543, 2920
PsuI RGATCY 6 cut(s) 355, 478, 823, 1202, 2548, 2591
PsyI GACNNNGTC 2 cut(s) 1740, 1749
PvuI CGATCG 1 cut(s) 607
RsaI GTAC 7 cut(s) 58, 1395, 1609, 1878, 2412, 3036, 3270
RsaNI GTAC 7 cut(s) 57, 1394, 1608, 1877, 2411, 3035, 3269
RseI CAYNNNNRTG 6 cut(s) 268, 1575, 1716, 2076, 2825, 3567
SacI GAGCTC 3 cut(s) 138, 1092, 2658
SalI GTCGAC 1 cut(s) 1743
SapI GCTCTTC 2 cut(s) 408, 451
SatI GCNGC 4 cut(s) 195, 711, 748, 2008
Sau96I GGNCC 6 cut(s) 126, 500, 1543, 2920, 3168, 3290
ScaI AGTACT 1 cut(s) 1395
SchI GAGTC 4 cut(s) 230, 1301, 1789, 2103
ScrFI CCNGG 6 cut(s) 984, 1548, 1762, 2511, 2930, 3500
SduI GDGCHC 6 cut(s) 8, 138, 1092, 2658, 3475, 3542
SfaNI GCATC 8 cut(s) 7, 877, 1498, 1567, 1635, 1641, 1811, 1833
SinI GGWCC 5 cut(s) 500, 1543, 2920, 3168, 3290
SmiMI CAYNNNNRTG 6 cut(s) 268, 1575, 1716, 2076, 2825, 3567
SmlI CTYRAG 7 cut(s) 73, 393, 1085, 2120, 2476, 2606, 3418
SmoI CTYRAG 7 cut(s) 73, 393, 1085, 2120, 2476, 2606, 3418
SphI GCATGC 1 cut(s) 1356
SsiI CCGC 1 cut(s) 1999
SspI AATATT 1 cut(s) 1588
SspMI CTAG 5 cut(s) 249, 1454, 1982, 2346, 2955
SstI GAGCTC 3 cut(s) 138, 1092, 2658
StyD4I CCNGG 6 cut(s) 982, 1546, 1760, 2509, 2928, 3498
StyI CCWWGG 2 cut(s) 129, 3153
TaaI ACNGT 6 cut(s) 12, 507, 1393, 1420, 1618, 2847
TaiI ACGT 6 cut(s) 100, 155, 307, 330, 2464, 3274
TaqI TCGA 9 cut(s) 174, 224, 613, 1501, 1527, 1744, 1952, 2171, 3061
TatI WGTACW 3 cut(s) 56, 1393, 1876
TscAI CASTG 4 cut(s) 345, 1425, 2678, 3414
TseFI GTSAC 1 cut(s) 2331
TseI GCWGC 4 cut(s) 194, 710, 747, 2007
Tsp45I GTSAC 1 cut(s) 2331
TspGWI ACGGA 2 cut(s) 573, 2985
TspRI CASTG 4 cut(s) 345, 1425, 2678, 3414
Tth111I GACNNNGTC 2 cut(s) 1740, 1749
Van91I CCANNNNNTGG 1 cut(s) 3104
Vha464I CTTAAG 1 cut(s) 2476
VpaK11BI GGWCC 5 cut(s) 500, 1543, 2920, 3168, 3290
VspI ATTAAT 1 cut(s) 1464
XbaI TCTAGA 1 cut(s) 248
XceI RCATGY 4 cut(s) 628, 1356, 3078, 3217
XmiI GTMKAC 1 cut(s) 1744
XmnI GAANNNNTTC 2 cut(s) 1729, 1902
XspI CTAG 5 cut(s) 249, 1454, 1982, 2346, 2955
ZrmI AGTACT 1 cut(s) 1395
Zsp2I ATGCAT 2 cut(s) 70, 3076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.