MD15G1375500.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000654
Physical Location & Seq
Forward (+)
45941682 .. 45942170
489 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1375500.v1.1.491

Sequence Viewer

Length: 369 bp
ATGAAGAGTCTTATTATGCTCGAGACTCTTTTGTTAGATAATTGCACAAACCTTGAAATTCTTCCAGAGCTCCCACCATGGTTGAAGAGGTTAGAAGCAAAATATTGTACTTCATTGAAAAGATTAACAAATTTACCAAACTTGTTCAGATCATTGGAATCACTTTTTTGGGGTTGTGAGCATTTAGTTGAAGTTGAAAGCTTGTTGAATATAAAACCGGTGAGAAGCGCTGACATAGAAATGACAAGATATATGGGCCTGTTCAATTTGAAATCCATAGCAAGCACTGATGTCGAAATGATCAACTACTTGACCAGTACAACCAGGAAGGCTCCTGTCCAGATTCTTGATGAAACAGGTACTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

123

Amino Acids

14.0

Weight (kDa)

5.42

Isoelectric Point (pI)

49.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 57, 130
AfaI GTAC 3 cut(s) 109, 319, 361
AfeI AGCGCT 1 cut(s) 229
AgeI ACCGGT 1 cut(s) 217
AgsI TTSAA 8 cut(s) 56, 85, 118, 191, 197, 208, 265, 271
AjnI CCWGG 1 cut(s) 323
AluBI AGCT 2 cut(s) 70, 201
AluI AGCT 2 cut(s) 70, 201
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 20
Aor51HI AGCGCT 1 cut(s) 229
AoxI GGCC 1 cut(s) 256
ApoI RAATTY 2 cut(s) 57, 130
AsiGI ACCGGT 1 cut(s) 217
Asp700I GAANNNNTTC 1 cut(s) 60
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 1 cut(s) 256
AsuHPI GGTGA 1 cut(s) 232
AvaI CYCGRG 1 cut(s) 20
BanII GRGCYC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 72
BcgI CGANNNNNNTGC 2 cut(s) 274, 308
BciT130I CCWGG 1 cut(s) 325
BclI TGATCA 1 cut(s) 300
BcoDI GTCTC 1 cut(s) 17
BfoI RGCGCY 1 cut(s) 231
Bme1390I CCNGG 1 cut(s) 325
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 256
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 1 cut(s) 325
BsaJI CCNNGG 1 cut(s) 77
BsaWI WCCGGW 1 cut(s) 217
Bse118I RCCGGY 1 cut(s) 217
Bse1I ACTGG 1 cut(s) 315
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 1 cut(s) 77
BseNI ACTGG 1 cut(s) 315
BshFI GGCC 1 cut(s) 258
BshTI ACCGGT 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 72
BsiHKCI CYCGRG 1 cut(s) 20
BsiSI CCGG 1 cut(s) 218
BsmAI GTCTC 1 cut(s) 17
BsnI GGCC 1 cut(s) 258
BsoBI CYCGRG 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 2 cut(s) 149, 300
Bsp19I CCATGG 1 cut(s) 77
BspANI GGCC 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 333
BsrFI RCCGGY 1 cut(s) 217
BsrI ACTGG 1 cut(s) 315
BssAI RCCGGY 1 cut(s) 217
BssECI CCNNGG 1 cut(s) 77
BssMI GATC 2 cut(s) 149, 300
BssT1I CCWWGG 1 cut(s) 77
Bst2UI CCWGG 1 cut(s) 325
Bst6I CTCTTC 1 cut(s) 80
BstC8I GCNNGC 1 cut(s) 283
BstDSI CCRYGG 1 cut(s) 77
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 2 cut(s) 152, 303
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 2 cut(s) 149, 300
BstNI CCWGG 1 cut(s) 325
BstSCI CCNGG 1 cut(s) 323
BsuRI GGCC 1 cut(s) 258
BtgI CCRYGG 1 cut(s) 77
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 1 cut(s) 283
CfoI GCGC 1 cut(s) 230
Cfr10I RCCGGY 1 cut(s) 217
Cfr13I GGNCC 1 cut(s) 256
Csp6I GTAC 3 cut(s) 108, 318, 360
CspAI ACCGGT 1 cut(s) 217
CspCI CAANNNNNGTGG 2 cut(s) 63, 98
CviAII CATG 1 cut(s) 78
CviJI RGCY 4 cut(s) 70, 201, 258, 332
CviKI_1 RGCY 4 cut(s) 70, 201, 258, 332
CviQI GTAC 3 cut(s) 108, 318, 360
DpnI GATC 2 cut(s) 151, 302
DpnII GATC 2 cut(s) 149, 300
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 77
Eco24I GRGCYC 1 cut(s) 72
Eco47III AGCGCT 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 70
Eco88I CYCGRG 1 cut(s) 20
EcoICRI GAGCTC 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 323
EcoT14I CCWWGG 1 cut(s) 77
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 77
FaeI CATG 1 cut(s) 81
FaiI YATR 8 cut(s) 17, 79, 212, 236, 252, 254, 278, 367
FatI CATG 1 cut(s) 77
FbaI TGATCA 1 cut(s) 300
FriOI GRGCYC 1 cut(s) 72
GlaI GCGC 1 cut(s) 229
HaeII RGCGCY 1 cut(s) 231
HaeIII GGCC 1 cut(s) 258
HapII CCGG 1 cut(s) 218
HhaI GCGC 1 cut(s) 230
Hin1II CATG 1 cut(s) 81
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HindIII AAGCTT 1 cut(s) 199
HinfI GANTC 4 cut(s) 7, 25, 158, 343
HpaII CCGG 1 cut(s) 218
HphI GGTGA 1 cut(s) 232
Hpy188I TCNGA 1 cut(s) 149
Hpy188III TCNNGA 4 cut(s) 22, 65, 340, 347
HpyAV CCTTC 1 cut(s) 322
HpyCH4V TGCA 1 cut(s) 45
Hsp92II CATG 1 cut(s) 81
HspAI GCGC 1 cut(s) 228
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 2 cut(s) 149, 300
LmnI GCTCC 2 cut(s) 75, 337
LpnPI CCDG 9 cut(s) 78, 231, 272, 310, 328, 337, 342, 348, 353
MalI GATC 2 cut(s) 151, 302
MboI GATC 2 cut(s) 149, 300
MboII GAAGA 3 cut(s) 16, 53, 97
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 4 cut(s) 40, 57, 130, 265
MlyI GAGTC 2 cut(s) 16, 19
MnlI CCTC 1 cut(s) 81
MroXI GAANNNNTTC 1 cut(s) 60
MseI TTAA 1 cut(s) 125
MslI CAYNNNNRTG 1 cut(s) 239
MspI CCGG 1 cut(s) 218
MspR9I CCNGG 1 cut(s) 325
MvaI CCWGG 1 cut(s) 325
NcoI CCATGG 1 cut(s) 77
NdeII GATC 2 cut(s) 149, 300
NlaIII CATG 1 cut(s) 81
NlaIV GGNNCC 1 cut(s) 333
PaeR7I CTCGAG 1 cut(s) 20
PdmI GAANNNNTTC 1 cut(s) 60
PfeI GAWTC 2 cut(s) 158, 343
PinAI ACCGGT 1 cut(s) 217
PleI GAGTC 2 cut(s) 15, 19
PpsI GAGTC 2 cut(s) 15, 19
Psp124BI GAGCTC 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 323
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 1 cut(s) 333
PspPI GGNCC 1 cut(s) 256
RsaI GTAC 3 cut(s) 109, 319, 361
RsaNI GTAC 3 cut(s) 108, 318, 360
RseI CAYNNNNRTG 1 cut(s) 239
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 1 cut(s) 125
Sau3AI GATC 2 cut(s) 149, 300
Sau96I GGNCC 1 cut(s) 256
SchI GAGTC 2 cut(s) 16, 19
ScrFI CCNGG 1 cut(s) 325
SduI GDGCHC 1 cut(s) 72
SetI ASST 5 cut(s) 54, 72, 92, 203, 361
Sfr274I CTCGAG 1 cut(s) 20
SlaI CTCGAG 1 cut(s) 20
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 4 cut(s) 40, 57, 130, 265
SspI AATATT 1 cut(s) 104
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 1 cut(s) 323
StyI CCWWGG 1 cut(s) 77
TaqI TCGA 2 cut(s) 21, 294
TasI AATT 4 cut(s) 40, 57, 130, 265
TatI WGTACW 2 cut(s) 107, 317
TfiI GAWTC 2 cut(s) 158, 343
Tru1I TTAA 1 cut(s) 125
Tru9I TTAA 1 cut(s) 125
TscAI CASTG 1 cut(s) 292
TspDTI ATGAA 3 cut(s) 17, 102, 366
TspRI CASTG 1 cut(s) 292
XapI RAATTY 2 cut(s) 57, 130
XhoI CTCGAG 1 cut(s) 20
XmnI GAANNNNTTC 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.