Prupe.3G130700_v2.0.a1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000654
Physical Location & Seq
Reverse (-)
13341403 .. 13344812
3410 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G130700.1

Sequence Viewer

Length: 2826 bp
ATGGCTGCCACCAGAGCGCAGCAGGATGCTTCTCCGTCCTCTCACTGGTGCACTTATGATGCATTCTTGAGCTTCAGGGCCACAGACACACGCAAGGGATTTGCTGATCACCTCTATAGGGCTTTGGAGGTGGCAGGGATCCACACTTTTAGAGATGATGATGAAATCGAGAGAGGAGCAAATATTTTAGCAGAGCTTCAAAAAGCAATACAAGAGTCTCGAGTATCCATCATTGTCTTCTCCAAGGACTACGCTTCCTCGAGATGGTGCCTGGATGAACGAGTGACGATCATGGACCGTAGAGAAACTAATGAGCACATGGTTATGCCGATTTTCTATGATGTGGATCCATTCCATCGCTTCAACAAGGAGACGGACAAGGTGGAGAAGTGTAGAAAAGCTCTCAGAGATGTTGCAGATTTGGGAGGGATGGTCTTAGGAGATCGGTCCGAGTCGCAGTTTATCCAAGAAATAGTTGAAGTTATTGGAAATAAACTGGATCACACATGGAATAGGAGATTAAGAGTCGATCCCTATGTAGTTGGAATAGATTATCGTGTGAAAGGCCTAAACATGTGGTTAGAAGATGGATCAAGTGATGTTGGAGTAGCTGTAGTCTATGGGATGGGTGGAATTGGCAAGACCACCATTGCCAAGACTGCTTATAACCAGAACTTCTATAAATTTCAAGGTAGCAGCTTTCTTGCAGATATTAGAGCAACTTCAAAACTTCCCAATGGTTTGGTTCACTTCCAAAAGAACCTTCTTTCAGATCTCCAAAAGGGGAAAGCAAAGAAAATATACAGCTTGGATGAAGGAATAACAAAGATCAAACAGGCGACACGTTGCAAAAGAGTTCTTATTGCTCTTGATGATGTGGACAACTTGGAACAATTCAATGCAATTCTTGGAATGCGAGAATGGCTTCATCCAGGAAGTAAAATAATCATAACAACTAGACATGAACATTTGTTAAAGGCTCATGAGAATTGTGCAATGTTTAAGGTGAAAGGGTTGAATGAGAATGAATCACTTGAGCTTTTCAGTTGGCATGCCTTTAGACAACCCCATCCTGGCGCAGGTTACATGGATCTTTCAAGACCTGTAGTGCAACACTGTGGAGGGGTTCCATTAGCTCTTCAAGTTCTAGGATCTTCTCTCTTTGGAAAAGCTGCAGATGTATGGAAAAATGCATTACAGAACTTGGATGTGATTACTGAAGGAAAAGTTCAAAAGATTCTCAGAATAAGCTTTGATTCTCTGCAAGATCATGACAAACGTCTGTTCCTCCATATAGCCTGTTACTTCATAGGAAAACAAAAGGATTTTTCAACTACAGTCCTTGATGAATGTGGGTTTGCCACAAACATTGGAATTCAAAATCTGAAGTTAACCATGCACCAATTACTTCAAGACATGGGAAGGGGAATTATTCGTGAAGAATCACCTGAGGATCCTGGAAAACGTACTAGAGTGTGGAATAAAAATGCCTCTAATGTTTTGAGAAAATTAACTGGTACAGAAACTATTAAGGGCCTCATGCTCAACATTCCTATGTTAATAAAAGATGAGTCTTCTAAGATAACATCCAGTGGAAGTAACAGAAAAAGATTCCATGTTGAAGATTATGATGGAAACTGTTCAAGCAGCCGACGTCGGCTTGGTTTCTTCTCTTGGCAGTCAATTAGTTTTTCTTCAACAAACTCATTTCCTGTATCAAATGGGATAGGTTTCAAAACAGAGGCATTTAGAAGGATGCACAATCTTGAACTCCTGCTGCTTGATAATGTAAAAATCAGTGGAGGCTATGAAGATTTCTCAAAAAATTTAATATGGTTGTCTTCGCGAGGATTCGCTTTAAAGTCAATACCAACCAATTTTCGCTTGGAAAATCTAATTGCTCTTGACTTGCGGAACAGCAGCCTCCAACATGTTTGGAAGGGAACTAAGTTTCTTCCAAGACTGAAAATCTTAAATCTCAGTCATTCACATGGATTTGTGACAACCCCTGACTTGTCAGGATTCCCTAACCTCGAGAGATTAATCCTTAAAGTTTGCATAAATTTGAAAGAGGTGGATGAATCCATTGGAGATTTGGAGAAACTTGTTTTTTTGAATCTAAAAGATTGCAAAAATCTCATGAAGCTTCCAATAAGAATTAGTATGCTGCAATCTCTCCAGAAACTTATTCTCTCTGGTTGCTCAAATCTTGTGCTGCCTGCCAGTATGATCGTTAAAAATCAGTTAGACTCTCCACCTAGTGACATGAAGAAAGTCTGTCTGTTATCTGCAGTTAAATCGTGGCAATCAATCCGATCATGGGTATTGCCAAGAAGATATCTCCAACTTACCAGTGCAAGTTTGCCACAATTTTTAAAAAGCTTAGATATGGCATACTGCAATCTGTCAGAAATTCCCAATGATCTTAGTAGCCTATCCTCATTGGAGTATTTGAAGCTAAGCGGAAACCCATTTTTGAGCCTATCAGTAAACATGAATGGTCTTAGTAAGCTCCAGACTCTTTTGTTGGATCGTTGCACAAACCCCGAAATGATTCCCGAGCTCCCACCAAGTGTAAAGACTTTGCGCGCATATAGATGTACTTCATTGAAAAGAGTACTACGAAACTTACCCAACATGATACCAAAAATTGGAAATGCGTTTTTGATATGCAAGAATTTAGTTGAAATTCAAACAATGTTCAAAACAAGACCGTTGAGAAGCGTCAACATAGAAATGATCAAAGATATCGGTCAGTTCAATTTGGAATCCATAGGAAGCACTGAAGTTGAAATGCTCAACTACTTGACATGTACAACAAGGAAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

942

Amino Acids

106.74

Weight (kDa)

9.23

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
AatII GACGTC 1 cut(s) 1657
Acc36I ACCTGC 1 cut(s) 1070
AccB1I GGYRCC 1 cut(s) 267
AccB7I CCANNNNNTGG 1 cut(s) 2651
AccII CGCG 2 cut(s) 1846, 2589
AciI CCGC 2 cut(s) 1912, 2463
AcsI RAATTY 7 cut(s) 683, 1374, 1825, 2062, 2412, 2677, 2688
AcuI CTGAAG 4 cut(s) 58, 1239, 1406, 2805
AcyI GRCGYC 1 cut(s) 1654
AdeI CACNNNGTG 2 cut(s) 2261, 2573
AfaI GTAC 5 cut(s) 1468, 1519, 2602, 2619, 2815
AfiI CCNNNNNNNGG 7 cut(s) 45, 118, 264, 1073, 1079, 2320, 2651
AflIII ACRYGT 4 cut(s) 573, 842, 1930, 2810
AjnI CCWGG 4 cut(s) 270, 931, 1072, 1456
AjuI GAANNNNNNNTTGG 2 cut(s) 747, 779
Alw21I GWGCWC 3 cut(s) 53, 318, 2565
Alw26I GTCTC 2 cut(s) 222, 365
Alw44I GTGCAC 1 cut(s) 49
Ama87I CYCGRG 4 cut(s) 219, 259, 2033, 2558
AoxI GGCC 3 cut(s) 78, 565, 1534
ApaLI GTGCAC 1 cut(s) 49
ApeKI GCWGC 9 cut(s) 5, 19, 696, 1172, 1647, 1777, 1920, 2167, 2215
ApoI RAATTY 7 cut(s) 683, 1374, 1825, 2062, 2412, 2677, 2688
AseI ATTAAT 1 cut(s) 2042
Asp700I GAANNNNTTC 3 cut(s) 924, 1639, 2553
AspLEI GCGC 4 cut(s) 19, 1079, 2589, 2591
AspS9I GGNCC 4 cut(s) 78, 295, 447, 1534
AsuHPI GGTGA 3 cut(s) 101, 1018, 1437
AvaI CYCGRG 4 cut(s) 219, 259, 2033, 2558
AvaII GGWCC 2 cut(s) 295, 447
AxyI CCTNAGG 1 cut(s) 1449
BaeGI GKGCMC 1 cut(s) 53
BamHI GGATCC 3 cut(s) 138, 346, 1453
BanI GGYRCC 1 cut(s) 267
BanII GRGCYC 1 cut(s) 2565
BbsI GAAGAC 3 cut(s) 229, 1566, 1833
Bbv12I GWGCWC 3 cut(s) 53, 318, 2565
BbvI GCAGC 8 cut(s) 31, 708, 1159, 1659, 1764, 1932, 2154, 2202
BccI CCATC 8 cut(s) 236, 258, 363, 424, 581, 619, 1077, 1625
BcgI CGANNNNNNTGC 2 cut(s) 2280, 2314
BciT130I CCWGG 4 cut(s) 272, 933, 1074, 1458
BciVI GTATCC 1 cut(s) 235
BclI TGATCA 2 cut(s) 106, 2739
BcoDI GTCTC 2 cut(s) 222, 365
BfaI CTAG 4 cut(s) 957, 1148, 1470, 2259
BfmI CTRYAG 6 cut(s) 115, 612, 1104, 1173, 1335, 2289
BfuAI ACCTGC 1 cut(s) 1070
BfuI GTATCC 1 cut(s) 235
BglII AGATCT 1 cut(s) 772
BisI GCNGC 9 cut(s) 6, 20, 697, 1173, 1648, 1778, 1921, 2168, 2216
BlpI GCTNAGC 1 cut(s) 2459
BlsI GCNGC 9 cut(s) 7, 21, 698, 1174, 1649, 1779, 1922, 2169, 2217
BmcAI AGTACT 1 cut(s) 2619
Bme1390I CCNGG 4 cut(s) 272, 933, 1074, 1458
Bme18I GGWCC 2 cut(s) 295, 447
BmeT110I CYCGRG 4 cut(s) 219, 259, 2033, 2558
BmgT120I GGNCC 4 cut(s) 78, 295, 447, 1534
BmiI GGNNCC 5 cut(s) 140, 269, 348, 1128, 1455
BmrFI CCNGG 4 cut(s) 272, 933, 1074, 1458
BmsI GCATC 3 cut(s) 16, 49, 1746
BoxI GACNNNNGTC 1 cut(s) 1278
BpiI GAAGAC 3 cut(s) 229, 1566, 1833
BpmI CTGGAG 2 cut(s) 2162, 2498
Bpu1102I GCTNAGC 1 cut(s) 2459
BpuEI CTTGAG 2 cut(s) 88, 1055
BsaBI GATNNNNATC 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 1654
BsaJI CCNNGG 1 cut(s) 243
BsaXI ACNNNNNCTCC 4 cut(s) 417, 447, 597, 627
Bsc4I CCNNNNNNNGG 7 cut(s) 45, 118, 264, 1073, 1079, 2320, 2651
Bse1I ACTGG 6 cut(s) 50, 501, 1519, 1590, 2223, 2352
Bse21I CCTNAGG 1 cut(s) 1449
Bse3DI GCAATG 2 cut(s) 648, 1002
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 4 cut(s) 272, 933, 1074, 1458
BseDI CCNNGG 1 cut(s) 243
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 7 cut(s) 45, 118, 264, 1073, 1079, 2320, 2651
BseMI GCAATG 2 cut(s) 648, 1002
BseMII CTCAG 4 cut(s) 418, 1255, 1440, 1993
BseNI ACTGG 6 cut(s) 50, 501, 1519, 1590, 2223, 2352
BsePI GCGCGC 1 cut(s) 2587
BseRI GAGGAG 1 cut(s) 189
BseSI GKGCMC 1 cut(s) 53
BseXI GCAGC 8 cut(s) 31, 708, 1159, 1659, 1764, 1932, 2154, 2202
Bsh1236I CGCG 2 cut(s) 1846, 2589
BshFI GGCC 3 cut(s) 80, 567, 1536
BshNI GGYRCC 1 cut(s) 267
BsiHKAI GWGCWC 3 cut(s) 53, 318, 2565
BsiHKCI CYCGRG 4 cut(s) 219, 259, 2033, 2558
BslI CCNNNNNNNGG 7 cut(s) 45, 118, 264, 1073, 1079, 2320, 2651
BsmAI GTCTC 2 cut(s) 222, 365
BsmBI CGTCTC 1 cut(s) 365
BsmI GAATGC 2 cut(s) 62, 918
BsnI GGCC 3 cut(s) 80, 567, 1536
BsoBI CYCGRG 4 cut(s) 219, 259, 2033, 2558
Bsp1286I GDGCHC 3 cut(s) 53, 318, 2565
Bsp1407I TGTACA 1 cut(s) 2813
Bsp1720I GCTNAGC 1 cut(s) 2459
Bsp68I TCGCGA 1 cut(s) 1846
BspACI CCGC 2 cut(s) 1912, 2463
BspANI GGCC 3 cut(s) 80, 567, 1536
BspCNI CTCAG 4 cut(s) 417, 1254, 1441, 1992
BspFNI CGCG 2 cut(s) 1846, 2589
BspHI TCATGA 3 cut(s) 982, 1270, 2139
BspLI GGNNCC 5 cut(s) 140, 269, 348, 1128, 1455
BspMAI CTGCAG 2 cut(s) 1177, 2293
BspMI ACCTGC 1 cut(s) 1070
BspQI GCTCTTC 1 cut(s) 1143
BspT107I GGYRCC 1 cut(s) 267
BsrDI GCAATG 2 cut(s) 648, 1002
BsrGI TGTACA 1 cut(s) 2813
BsrI ACTGG 6 cut(s) 50, 501, 1519, 1590, 2223, 2352
BssECI CCNNGG 1 cut(s) 243
BssHII GCGCGC 1 cut(s) 2587
BssNI GRCGYC 1 cut(s) 1654
BssT1I CCWWGG 1 cut(s) 243
Bst2UI CCWGG 4 cut(s) 272, 933, 1074, 1458
Bst4CI ACNGT 5 cut(s) 299, 1118, 1339, 1640, 2715
Bst6I CTCTTC 1 cut(s) 1143
BstACI GRCGYC 1 cut(s) 1654
BstAUI TGTACA 1 cut(s) 2813
BstC8I GCNNGC 3 cut(s) 1053, 2220, 2589
BstENI CCTNNNNNAGG 1 cut(s) 1077
BstFNI CGCG 2 cut(s) 1846, 2589
BstHHI GCGC 4 cut(s) 19, 1079, 2589, 2591
BstMAI GTCTC 2 cut(s) 222, 365
BstMWI GCNNNNNNNGC 3 cut(s) 14, 659, 922
BstNI CCWGG 4 cut(s) 272, 933, 1074, 1458
BstNSI RCATGY 4 cut(s) 577, 1055, 1934, 2814
BstPAI GACNNNNGTC 1 cut(s) 1278
BstSCI CCNGG 4 cut(s) 270, 931, 1072, 1456
BstSFI CTRYAG 6 cut(s) 115, 612, 1104, 1173, 1335, 2289
BstSLI GKGCMC 1 cut(s) 53
BstUI CGCG 2 cut(s) 1846, 2589
BstV1I GCAGC 8 cut(s) 31, 708, 1159, 1659, 1764, 1932, 2154, 2202
BstV2I GAAGAC 3 cut(s) 229, 1566, 1833
BstX2I RGATCY 6 cut(s) 138, 346, 772, 1090, 1151, 1453
BstXI CCANNNNNNTGG 1 cut(s) 742
BstYI RGATCY 6 cut(s) 138, 346, 772, 1090, 1151, 1453
Bsu36I CCTNAGG 1 cut(s) 1449
BsuI GTATCC 1 cut(s) 235
BsuRI GGCC 3 cut(s) 80, 567, 1536
BtgZI GCGATG 1 cut(s) 341
BtsIMutI CAGTG 6 cut(s) 43, 1114, 1597, 1804, 2359, 2781
BtuMI TCGCGA 1 cut(s) 1846
BveI ACCTGC 1 cut(s) 1070
Cac8I GCNNGC 3 cut(s) 1053, 2220, 2589
CciI TCATGA 3 cut(s) 982, 1270, 2139
CfoI GCGC 4 cut(s) 19, 1079, 2589, 2591
Cfr13I GGNCC 4 cut(s) 78, 295, 447, 1534
CpoI CGGWCCG 1 cut(s) 447
CseI GACGC 1 cut(s) 2713
Csp6I GTAC 5 cut(s) 1467, 1518, 2601, 2618, 2814
CspCI CAANNNNNGTGG 2 cut(s) 1351, 1386
CspI CGGWCCG 1 cut(s) 447
CviQI GTAC 5 cut(s) 1467, 1518, 2601, 2618, 2814
DraI TTTAAA 2 cut(s) 1860, 2376
DraIII CACNNNGTG 2 cut(s) 2261, 2573
Eam1104I CTCTTC 1 cut(s) 1143
EarI CTCTTC 1 cut(s) 1143
Ecl136II GAGCTC 1 cut(s) 2563
Eco130I CCWWGG 1 cut(s) 243
Eco147I AGGCCT 1 cut(s) 567
Eco24I GRGCYC 1 cut(s) 2565
Eco32I GATATC 2 cut(s) 2339, 2749
Eco47I GGWCC 2 cut(s) 295, 447
Eco53kI GAGCTC 1 cut(s) 2563
Eco57I CTGAAG 4 cut(s) 58, 1239, 1406, 2805
Eco81I CCTNAGG 1 cut(s) 1449
Eco88I CYCGRG 4 cut(s) 219, 259, 2033, 2558
EcoICRI GAGCTC 1 cut(s) 2563
EcoNI CCTNNNNNAGG 1 cut(s) 1077
EcoO109I RGGNCCY 1 cut(s) 1534
EcoRI GAATTC 1 cut(s) 1374
EcoRII CCWGG 4 cut(s) 270, 931, 1072, 1456
EcoRV GATATC 2 cut(s) 2339, 2749
EcoT14I CCWWGG 1 cut(s) 243
EcoT22I ATGCAT 2 cut(s) 64, 1195
EcoT38I GRGCYC 1 cut(s) 2565
ErhI CCWWGG 1 cut(s) 243
Esp3I CGTCTC 1 cut(s) 365
FbaI TGATCA 2 cut(s) 106, 2739
Fnu4HI GCNGC 9 cut(s) 6, 20, 697, 1173, 1648, 1778, 1921, 2168, 2216
FriOI GRGCYC 1 cut(s) 2565
Fsp4HI GCNGC 9 cut(s) 6, 20, 697, 1173, 1648, 1778, 1921, 2168, 2216
FspBI CTAG 4 cut(s) 957, 1148, 1470, 2259
GlaI GCGC 4 cut(s) 18, 1078, 2588, 2590
GluI GCNGC 9 cut(s) 6, 20, 697, 1173, 1648, 1778, 1921, 2168, 2216
GsuI CTGGAG 2 cut(s) 2162, 2498
HaeIII GGCC 3 cut(s) 80, 567, 1536
HgaI GACGC 1 cut(s) 2713
HhaI GCGC 4 cut(s) 19, 1079, 2589, 2591
Hin1I GRCGYC 1 cut(s) 1654
Hin6I GCGC 4 cut(s) 17, 1077, 2587, 2589
HinP1I GCGC 4 cut(s) 17, 1077, 2587, 2589
HincII GTYRAC 2 cut(s) 1392, 2728
HindII GTYRAC 2 cut(s) 1392, 2728
HindIII AAGCTT 3 cut(s) 1249, 2144, 2380
HpaI GTTAAC 1 cut(s) 1392
HphI GGTGA 3 cut(s) 101, 1018, 1437
Hpy166II GTNNAC 6 cut(s) 51, 748, 880, 1392, 2491, 2728
Hpy188I TCNGA 7 cut(s) 407, 451, 772, 1244, 1386, 2315, 2410
Hpy8I GTNNAC 6 cut(s) 51, 748, 880, 1392, 2491, 2728
Hpy99I CGWCG 2 cut(s) 1656, 1659
HpyAV CCTTC 6 cut(s) 773, 809, 1214, 1416, 1746, 1933
HpyCH4III ACNGT 5 cut(s) 299, 1118, 1339, 1640, 2715
HpyCH4IV ACGT 4 cut(s) 844, 1279, 1465, 1654
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 659, 922
HpySE526I ACGT 4 cut(s) 844, 1279, 1465, 1654
Hsp92I GRCGYC 1 cut(s) 1654
HspAI GCGC 4 cut(s) 17, 1077, 2587, 2589
Ksp22I TGATCA 2 cut(s) 106, 2739
KspAI GTTAAC 1 cut(s) 1392
LguI GCTCTTC 1 cut(s) 1143
LmnI GCTCC 3 cut(s) 176, 2517, 2568
Lsp1109I GCAGC 8 cut(s) 31, 708, 1159, 1659, 1764, 1932, 2154, 2202
LweI GCATC 3 cut(s) 16, 49, 1746
MaeI CTAG 4 cut(s) 957, 1148, 1470, 2259
MaeII ACGT 4 cut(s) 844, 1279, 1465, 1654
MaeIII GTNAC 6 cut(s) 283, 1082, 1301, 1598, 1999, 2261
MflI RGATCY 6 cut(s) 138, 346, 772, 1090, 1151, 1453
MhlI GDGCHC 3 cut(s) 53, 318, 2565
MlyI GAGTC 6 cut(s) 224, 461, 534, 1580, 2243, 2512
MmeI TCCRAC 5 cut(s) 523, 583, 1951, 2368, 2508
Mph1103I ATGCAT 2 cut(s) 64, 1195
MroXI GAANNNNTTC 3 cut(s) 924, 1639, 2553
MslI CAYNNNNRTG 5 cut(s) 323, 1553, 1989, 2596, 2735
MspR9I CCNGG 4 cut(s) 272, 933, 1074, 1458
Mva1269I GAATGC 2 cut(s) 62, 918
MvaI CCWGG 4 cut(s) 272, 933, 1074, 1458
MvnI CGCG 2 cut(s) 1846, 2589
MwoI GCNNNNNNNGC 3 cut(s) 14, 659, 922
NlaIV GGNNCC 5 cut(s) 140, 269, 348, 1128, 1455
NmuCI GTSAC 3 cut(s) 283, 1999, 2261
NruI TCGCGA 1 cut(s) 1846
NsiI ATGCAT 2 cut(s) 64, 1195
NspI RCATGY 4 cut(s) 577, 1055, 1934, 2814
PaeI GCATGC 1 cut(s) 1055
PaeR7I CTCGAG 3 cut(s) 219, 259, 2033
PagI TCATGA 3 cut(s) 982, 1270, 2139
PauI GCGCGC 1 cut(s) 2587
PceI AGGCCT 1 cut(s) 567
PciI ACATGT 3 cut(s) 573, 1930, 2810
PciSI GCTCTTC 1 cut(s) 1143
PctI GAATGC 2 cut(s) 62, 918
PdmI GAANNNNTTC 3 cut(s) 924, 1639, 2553
PflMI CCANNNNNTGG 1 cut(s) 2651
PfoI TCCNGGA 2 cut(s) 931, 1456
PkrI GCNGC 9 cut(s) 7, 21, 698, 1174, 1649, 1779, 1922, 2169, 2217
PleI GAGTC 6 cut(s) 223, 460, 533, 1579, 2243, 2512
PpsI GAGTC 6 cut(s) 223, 460, 533, 1579, 2243, 2512
PscI ACATGT 3 cut(s) 573, 1930, 2810
PshAI GACNNNNGTC 1 cut(s) 1278
PshBI ATTAAT 1 cut(s) 2042
PsiI TTATAA 1 cut(s) 666
Psp124BI GAGCTC 1 cut(s) 2565
Psp6I CCWGG 4 cut(s) 270, 931, 1072, 1456
PspGI CCWGG 4 cut(s) 270, 931, 1072, 1456
PspN4I GGNNCC 5 cut(s) 140, 269, 348, 1128, 1455
PspPI GGNCC 4 cut(s) 78, 295, 447, 1534
PstI CTGCAG 2 cut(s) 1177, 2293
PsuI RGATCY 6 cut(s) 138, 346, 772, 1090, 1151, 1453
PteI GCGCGC 1 cut(s) 2587
RruI TCGCGA 1 cut(s) 1846
RsaI GTAC 5 cut(s) 1468, 1519, 2602, 2619, 2815
RsaNI GTAC 5 cut(s) 1467, 1518, 2601, 2618, 2814
RseI CAYNNNNRTG 5 cut(s) 323, 1553, 1989, 2596, 2735
Rsr2I CGGWCCG 1 cut(s) 447
RsrII CGGWCCG 1 cut(s) 447
SacI GAGCTC 1 cut(s) 2565
SapI GCTCTTC 1 cut(s) 1143
SatI GCNGC 9 cut(s) 6, 20, 697, 1173, 1648, 1778, 1921, 2168, 2216
Sau96I GGNCC 4 cut(s) 78, 295, 447, 1534
ScaI AGTACT 1 cut(s) 2619
SchI GAGTC 6 cut(s) 224, 461, 534, 1580, 2243, 2512
ScrFI CCNGG 4 cut(s) 272, 933, 1074, 1458
SduI GDGCHC 3 cut(s) 53, 318, 2565
SfaNI GCATC 3 cut(s) 16, 49, 1746
SfcI CTRYAG 6 cut(s) 115, 612, 1104, 1173, 1335, 2289
Sfr274I CTCGAG 3 cut(s) 219, 259, 2033
SinI GGWCC 2 cut(s) 295, 447
SlaI CTCGAG 3 cut(s) 219, 259, 2033
SmiMI CAYNNNNRTG 5 cut(s) 323, 1553, 1989, 2596, 2735
SmlI CTYRAG 5 cut(s) 67, 219, 259, 1034, 2033
SmoI CTYRAG 5 cut(s) 67, 219, 259, 1034, 2033
SphI GCATGC 1 cut(s) 1055
SseBI AGGCCT 1 cut(s) 567
SsiI CCGC 2 cut(s) 1912, 2463
SspI AATATT 1 cut(s) 184
SspMI CTAG 4 cut(s) 957, 1148, 1470, 2259
SstI GAGCTC 1 cut(s) 2565
StuI AGGCCT 1 cut(s) 567
StyD4I CCNGG 4 cut(s) 270, 931, 1072, 1456
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 5 cut(s) 299, 1118, 1339, 1640, 2715
TaiI ACGT 4 cut(s) 847, 1282, 1468, 1657
TaqI TCGA 5 cut(s) 168, 220, 260, 528, 2034
TaqII GACCGA 2 cut(s) 435, 2741
TatI WGTACW 3 cut(s) 2600, 2617, 2813
TscAI CASTG 6 cut(s) 50, 1121, 1597, 1804, 2359, 2788
TseFI GTSAC 3 cut(s) 283, 1999, 2261
TseI GCWGC 9 cut(s) 5, 19, 696, 1172, 1647, 1777, 1920, 2167, 2215
Tsp45I GTSAC 3 cut(s) 283, 1999, 2261
TspGWI ACGGA 2 cut(s) 24, 389
TspRI CASTG 6 cut(s) 50, 1121, 1597, 1804, 2359, 2788
Van91I CCANNNNNTGG 1 cut(s) 2651
VneI GTGCAC 1 cut(s) 49
VpaK11BI GGWCC 2 cut(s) 295, 447
VspI ATTAAT 1 cut(s) 2042
XagI CCTNNNNNAGG 1 cut(s) 1077
XapI RAATTY 7 cut(s) 683, 1374, 1825, 2062, 2412, 2677, 2688
XceI RCATGY 4 cut(s) 577, 1055, 1934, 2814
XcmI CCANNNNNNNNNTGG 2 cut(s) 1882, 2092
XhoI CTCGAG 3 cut(s) 219, 259, 2033
XmnI GAANNNNTTC 3 cut(s) 924, 1639, 2553
XspI CTAG 4 cut(s) 957, 1148, 1470, 2259
ZraI GACGTC 1 cut(s) 1655
ZrmI AGTACT 1 cut(s) 2619
Zsp2I ATGCAT 2 cut(s) 64, 1195
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.