Rorug04G0058200

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000654
Physical Location & Seq
Reverse (-)
9120169 .. 9122266
2098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0058200.1

Sequence Viewer

Length: 687 bp
ATGGTGCCACTTGACCCAGCGTCCTGTCTCGTCGACGACCTCCGTAACTTCTTGTCCGACGTCGGCGACCACGACGGCCGGCCCGATGATCCTAGCCGTCCGTTAATCGCAACTGAGGAAGCAGAAGAAGAGCTGGAATGGATATCGAACAAGGACGCGTTCCCGGCGGTGGAAACGTTCATTCTCTCGGAGCAGGTGGGCGGCATTGCGATTGCCAAGCACCAGAGCCCTGTCTCGGTGCTGGAGAACAGCACAAACAGCAGCAGCGCCTCCCTCATGAGCTCGTGTGGGGGGCTAAAGCCGCCCCACCGAGCGCGAACCAAAGGCCGCCGCAGGCGGAGTGAAATTCCTGCGCAGCAGCTATTTTGGAACCAACCGATTGAGAGCAAACCCAGCAGGAGTCACAGTGCCAGTGCCAGTGCCAGCAAGCTGGATATTGGGAGAAAGTGCCTGCATTGCGGGACTGATCAGACACCGCAATGGCGGGCGGGTCCCGTTGGACCCAAAACCTTGTGTAATGCCTGTGGGGTGAGGTACAAGTCAGGGCGGCTTTGCCCCGAGTACCGCCCTGCGAGTAGCCCGACGTTCTCGAACGAGATGCATTCGAACTCCCACAGGAAGGTATTGGAGATGAGGAAGCAAAAGTATGGAATGGGGATGGTTGTGAAGCCTGAGGATACAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

228

Amino Acids

25.0

Weight (kDa)

8.47

Isoelectric Point (pI)

67.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATA PF00320 150 - 184 1.6e-15 GATA zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 184
AatII GACGTC 1 cut(s) 63
Acc16I TGCGCA 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 184
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 33
AccII CGCG 2 cut(s) 158, 316
AclI AACGTT 1 cut(s) 176
AclWI GGATC 1 cut(s) 83
AcoI YGGCCR 1 cut(s) 76
AcsI RAATTY 1 cut(s) 345
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 2 cut(s) 536, 563
AfiI CCNNNNNNNGG 3 cut(s) 169, 235, 619
AflIII ACRYGT 1 cut(s) 156
AhdI GACNNNNNGTC 1 cut(s) 19
AluBI AGCT 4 cut(s) 133, 282, 361, 430
AluI AGCT 4 cut(s) 133, 282, 361, 430
Alw21I GWGCWC 1 cut(s) 284
Alw26I GTCTC 2 cut(s) 32, 238
AlwI GGATC 1 cut(s) 83
Ama87I CYCGRG 1 cut(s) 557
AoxI GGCC 3 cut(s) 76, 80, 325
ApeKI GCWGC 4 cut(s) 261, 264, 355, 358
ApoI RAATTY 1 cut(s) 345
AspLEI GCGC 3 cut(s) 269, 316, 355
AspS9I GGNCC 3 cut(s) 81, 491, 500
AsuC2I CCSGG 1 cut(s) 164
AsuHPI GGTGA 1 cut(s) 541
AsuII TTCGAA 1 cut(s) 605
AvaI CYCGRG 1 cut(s) 557
AvaII GGWCC 2 cut(s) 491, 500
AxyI CCTNAGG 1 cut(s) 672
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 230, 284
BauI CACGAG 1 cut(s) 283
Bbv12I GWGCWC 1 cut(s) 284
BbvI GCAGC 4 cut(s) 273, 276, 367, 370
BccI CCATC 1 cut(s) 652
BceAI ACGGC 2 cut(s) 81, 91
BcgI CGANNNNNNTGC 2 cut(s) 580, 614
BciVI GTATCC 1 cut(s) 670
BclI TGATCA 1 cut(s) 466
BcnI CCSGG 1 cut(s) 164
BcoDI GTCTC 2 cut(s) 32, 238
BfaI CTAG 1 cut(s) 93
BfoI RGCGCY 1 cut(s) 270
BfuAI ACCTGC 1 cut(s) 184
BfuI GTATCC 1 cut(s) 670
BisI GCNGC 9 cut(s) 202, 262, 265, 302, 328, 331, 356, 359, 548
BlsI GCNGC 9 cut(s) 203, 263, 266, 303, 329, 332, 357, 360, 549
Bme1390I CCNGG 1 cut(s) 164
Bme18I GGWCC 2 cut(s) 491, 500
BmeRI GACNNNNNGTC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 557
BmgT120I GGNCC 3 cut(s) 81, 491, 500
BmiI GGNNCC 5 cut(s) 6, 371, 492, 493, 502
BmrFI CCNGG 1 cut(s) 164
BmsI GCATC 1 cut(s) 588
BpmI CTGGAG 1 cut(s) 263
Bpu14I TTCGAA 1 cut(s) 605
BpuMI CCSGG 1 cut(s) 164
BsaHI GRCGYC 1 cut(s) 60
BsaXI ACNNNNNCTCC 2 cut(s) 391, 421
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 235, 619
Bse118I RCCGGY 1 cut(s) 78
Bse1I ACTGG 2 cut(s) 411, 417
Bse21I CCTNAGG 1 cut(s) 672
Bse3DI GCAATG 3 cut(s) 204, 454, 485
BseGI GGATG 1 cut(s) 663
BseLI CCNNNNNNNGG 3 cut(s) 169, 235, 619
BseMI GCAATG 3 cut(s) 204, 454, 485
BseMII CTCAG 2 cut(s) 105, 663
BseNI ACTGG 2 cut(s) 411, 417
BseX3I CGGCCG 1 cut(s) 76
BseXI GCAGC 4 cut(s) 273, 276, 367, 370
BseYI CCCAGC 2 cut(s) 16, 392
Bsh1236I CGCG 2 cut(s) 158, 316
Bsh1285I CGRYCG 1 cut(s) 79
BshFI GGCC 3 cut(s) 78, 82, 327
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 79
BsiHKAI GWGCWC 1 cut(s) 284
BsiHKCI CYCGRG 1 cut(s) 557
BsiSI CCGG 2 cut(s) 79, 164
BslFI GGGAC 2 cut(s) 475, 477
BslI CCNNNNNNNGG 3 cut(s) 169, 235, 619
BsmAI GTCTC 2 cut(s) 32, 238
BsmFI GGGAC 2 cut(s) 475, 477
BsmI GAATGC 1 cut(s) 601
BsnI GGCC 3 cut(s) 78, 82, 327
BsoBI CYCGRG 1 cut(s) 557
Bsp119I TTCGAA 1 cut(s) 605
Bsp1286I GDGCHC 2 cut(s) 230, 284
Bsp143I GATC 2 cut(s) 88, 466
BspANI GGCC 3 cut(s) 78, 82, 327
BspCNI CTCAG 2 cut(s) 106, 664
BspFNI CGCG 2 cut(s) 158, 316
BspHI TCATGA 1 cut(s) 276
BspLI GGNNCC 5 cut(s) 6, 371, 492, 493, 502
BspMI ACCTGC 1 cut(s) 184
BspPI GGATC 1 cut(s) 83
BspQI GCTCTTC 1 cut(s) 123
BspT104I TTCGAA 1 cut(s) 605
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 3 cut(s) 204, 454, 485
BsrFI RCCGGY 1 cut(s) 78
BsrI ACTGG 2 cut(s) 411, 417
BssAI RCCGGY 1 cut(s) 78
BssMI GATC 2 cut(s) 88, 466
BssNI GRCGYC 1 cut(s) 60
BssSI CACGAG 1 cut(s) 283
Bst2BI CACGAG 1 cut(s) 283
Bst4CI ACNGT 1 cut(s) 407
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 1 cut(s) 60
BstBI TTCGAA 1 cut(s) 605
BstC8I GCNNGC 6 cut(s) 80, 335, 424, 428, 452, 486
BstDEI CTNAG 2 cut(s) 114, 672
BstF5I GGATG 1 cut(s) 663
BstFNI CGCG 2 cut(s) 158, 316
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 3 cut(s) 269, 316, 355
BstKTI GATC 2 cut(s) 91, 469
BstMAI GTCTC 2 cut(s) 32, 238
BstMBI GATC 2 cut(s) 88, 466
BstMCI CGRYCG 1 cut(s) 79
BstMWI GCNNNNNNNGC 5 cut(s) 164, 258, 301, 393, 456
BstSCI CCNGG 1 cut(s) 162
BstUI CGCG 2 cut(s) 158, 316
BstV1I GCAGC 4 cut(s) 273, 276, 367, 370
BstXI CCANNNNNNTGG 1 cut(s) 430
BstZI CGGCCG 1 cut(s) 76
Bsu36I CCTNAGG 1 cut(s) 672
BsuI GTATCC 1 cut(s) 670
BsuRI GGCC 3 cut(s) 78, 82, 327
BtsCI GGATG 1 cut(s) 663
BtsIMutI CAGTG 3 cut(s) 412, 418, 424
BveI ACCTGC 1 cut(s) 184
Cac8I GCNNGC 6 cut(s) 80, 335, 424, 428, 452, 486
CciI TCATGA 1 cut(s) 276
CfoI GCGC 3 cut(s) 269, 316, 355
Cfr10I RCCGGY 1 cut(s) 78
Cfr13I GGNCC 3 cut(s) 81, 491, 500
CseI GACGC 2 cut(s) 9, 164
Csp6I GTAC 2 cut(s) 535, 562
CviAII CATG 1 cut(s) 277
CviQI GTAC 2 cut(s) 535, 562
DdeI CTNAG 2 cut(s) 114, 672
DpnI GATC 2 cut(s) 90, 468
DpnII GATC 2 cut(s) 88, 466
DriI GACNNNNNGTC 1 cut(s) 19
EaeI YGGCCR 1 cut(s) 76
EagI CGGCCG 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 123
Eam1105I GACNNNNNGTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 123
EciI GGCGGA 1 cut(s) 352
Ecl136II GAGCTC 1 cut(s) 282
EclXI CGGCCG 1 cut(s) 76
Eco24I GRGCYC 2 cut(s) 230, 284
Eco32I GATATC 1 cut(s) 144
Eco47I GGWCC 2 cut(s) 491, 500
Eco52I CGGCCG 1 cut(s) 76
Eco53kI GAGCTC 1 cut(s) 282
Eco81I CCTNAGG 1 cut(s) 672
Eco88I CYCGRG 1 cut(s) 557
EcoICRI GAGCTC 1 cut(s) 282
EcoO109I RGGNCCY 1 cut(s) 491
EcoRV GATATC 1 cut(s) 144
EcoT22I ATGCAT 1 cut(s) 603
EcoT38I GRGCYC 2 cut(s) 230, 284
FaeI CATG 1 cut(s) 280
FaiI YATR 2 cut(s) 278, 648
FaqI GGGAC 2 cut(s) 475, 477
FatI CATG 1 cut(s) 276
FauI CCCGC 3 cut(s) 452, 477, 481
FbaI TGATCA 1 cut(s) 466
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 9 cut(s) 202, 262, 265, 302, 328, 331, 356, 359, 548
FokI GGATG 1 cut(s) 670
FriOI GRGCYC 2 cut(s) 230, 284
FseI GGCCGGCC 1 cut(s) 82
Fsp4HI GCNGC 9 cut(s) 202, 262, 265, 302, 328, 331, 356, 359, 548
FspBI CTAG 1 cut(s) 93
FspI TGCGCA 1 cut(s) 354
GlaI GCGC 3 cut(s) 268, 315, 354
GluI GCNGC 9 cut(s) 202, 262, 265, 302, 328, 331, 356, 359, 548
GsaI CCCAGC 2 cut(s) 20, 396
GsuI CTGGAG 1 cut(s) 263
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 3 cut(s) 78, 82, 327
HapII CCGG 2 cut(s) 79, 164
HgaI GACGC 2 cut(s) 9, 164
HhaI GCGC 3 cut(s) 269, 316, 355
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 1 cut(s) 280
Hin6I GCGC 3 cut(s) 267, 314, 353
HinP1I GCGC 3 cut(s) 267, 314, 353
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HinfI GANTC 1 cut(s) 400
HpaII CCGG 2 cut(s) 79, 164
HphI GGTGA 1 cut(s) 541
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 3 cut(s) 58, 190, 471
Hpy188III TCNNGA 2 cut(s) 277, 589
Hpy8I GTNNAC 1 cut(s) 34
Hpy99I CGWCG 6 cut(s) 35, 38, 62, 65, 77, 586
HpyAV CCTTC 1 cut(s) 613
HpyCH4III ACNGT 1 cut(s) 407
HpyCH4IV ACGT 3 cut(s) 60, 176, 584
HpyCH4V TGCA 2 cut(s) 454, 601
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 258, 301, 393, 456
HpyF3I CTNAG 2 cut(s) 114, 672
HpySE526I ACGT 3 cut(s) 60, 176, 584
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 1 cut(s) 280
HspAI GCGC 3 cut(s) 267, 314, 353
KflI GGGWCCC 1 cut(s) 491
KroI GCCGGC 1 cut(s) 78
KroNI GCCGGC 1 cut(s) 80
Ksp22I TGATCA 1 cut(s) 466
Kzo9I GATC 2 cut(s) 88, 466
LguI GCTCTTC 1 cut(s) 123
LmnI GCTCC 1 cut(s) 190
Lsp1109I GCAGC 4 cut(s) 273, 276, 367, 370
LweI GCATC 1 cut(s) 588
MaeI CTAG 1 cut(s) 93
MaeII ACGT 3 cut(s) 60, 176, 584
MaeIII GTNAC 2 cut(s) 44, 401
MalI GATC 2 cut(s) 90, 468
MboI GATC 2 cut(s) 88, 466
MboII GAAGA 2 cut(s) 137, 140
MhlI GDGCHC 2 cut(s) 230, 284
MluCI AATT 1 cut(s) 345
MluI ACGCGT 1 cut(s) 156
MlyI GAGTC 1 cut(s) 409
MmeI TCCRAC 2 cut(s) 81, 478
MnlI CCTC 7 cut(s) 50, 109, 280, 284, 525, 627, 667
Mph1103I ATGCAT 1 cut(s) 603
MroNI GCCGGC 1 cut(s) 78
MseI TTAA 1 cut(s) 104
MslI CAYNNNNRTG 1 cut(s) 478
MspI CCGG 2 cut(s) 79, 164
MspR9I CCNGG 1 cut(s) 164
Mva1269I GAATGC 1 cut(s) 601
MvnI CGCG 2 cut(s) 158, 316
MwoI GCNNNNNNNGC 5 cut(s) 164, 258, 301, 393, 456
NaeI GCCGGC 1 cut(s) 80
NciI CCSGG 1 cut(s) 164
NdeII GATC 2 cut(s) 88, 466
NgoMIV GCCGGC 1 cut(s) 78
NlaIII CATG 1 cut(s) 280
NlaIV GGNNCC 5 cut(s) 6, 371, 492, 493, 502
NmuCI GTSAC 1 cut(s) 401
NsbI TGCGCA 1 cut(s) 354
NsiI ATGCAT 1 cut(s) 603
NspV TTCGAA 1 cut(s) 605
PagI TCATGA 1 cut(s) 276
PaqCI CACCTGC 1 cut(s) 184
PciSI GCTCTTC 1 cut(s) 123
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 81
PctI GAATGC 1 cut(s) 601
PdiI GCCGGC 1 cut(s) 80
PkrI GCNGC 9 cut(s) 203, 263, 266, 303, 329, 332, 357, 360, 549
PleI GAGTC 1 cut(s) 408
PpsI GAGTC 1 cut(s) 408
PpuMI RGGWCCY 1 cut(s) 491
Psp124BI GAGCTC 1 cut(s) 284
Psp1406I AACGTT 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 491
PspFI CCCAGC 2 cut(s) 16, 392
PspN4I GGNNCC 5 cut(s) 6, 371, 492, 493, 502
PspPI GGNCC 3 cut(s) 81, 491, 500
PspPPI RGGWCCY 1 cut(s) 491
RigI GGCCGGCC 1 cut(s) 82
RsaI GTAC 2 cut(s) 536, 563
RsaNI GTAC 2 cut(s) 535, 562
RseI CAYNNNNRTG 1 cut(s) 478
SacI GAGCTC 1 cut(s) 284
SalI GTCGAC 1 cut(s) 32
SapI GCTCTTC 1 cut(s) 123
SaqAI TTAA 1 cut(s) 104
SatI GCNGC 9 cut(s) 202, 262, 265, 302, 328, 331, 356, 359, 548
Sau3AI GATC 2 cut(s) 88, 466
Sau96I GGNCC 3 cut(s) 81, 491, 500
SchI GAGTC 1 cut(s) 409
ScrFI CCNGG 1 cut(s) 164
SduI GDGCHC 2 cut(s) 230, 284
SfaNI GCATC 1 cut(s) 588
SfuI TTCGAA 1 cut(s) 605
SgrDI CGTCGACG 1 cut(s) 32
SinI GGWCC 2 cut(s) 491, 500
SmiMI CAYNNNNRTG 1 cut(s) 478
Sse9I AATT 1 cut(s) 345
SspMI CTAG 1 cut(s) 93
SstI GAGCTC 1 cut(s) 284
StyD4I CCNGG 1 cut(s) 162
TaaI ACNGT 1 cut(s) 407
TaiI ACGT 3 cut(s) 63, 179, 587
TaqI TCGA 4 cut(s) 33, 146, 590, 605
TasI AATT 1 cut(s) 345
TauI GCSGC 5 cut(s) 204, 304, 330, 333, 550
Tru1I TTAA 1 cut(s) 104
Tru9I TTAA 1 cut(s) 104
TscAI CASTG 3 cut(s) 412, 418, 424
TseFI GTSAC 1 cut(s) 401
TseI GCWGC 4 cut(s) 261, 264, 355, 358
Tsp45I GTSAC 1 cut(s) 401
TspDTI ATGAA 1 cut(s) 169
TspGWI ACGGA 2 cut(s) 32, 90
TspRI CASTG 3 cut(s) 412, 418, 424
VpaK11BI GGWCC 2 cut(s) 491, 500
XapI RAATTY 1 cut(s) 345
XmiI GTMKAC 1 cut(s) 33
XspI CTAG 1 cut(s) 93
ZraI GACGTC 1 cut(s) 61
Zsp2I ATGCAT 1 cut(s) 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.