Rh6AG292000

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000654
Physical Location & Seq
Reverse (-)
48899050 .. 48919940
20891 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG292000.1

Sequence Viewer

Length: 2916 bp
ATGGAACGTAAAAGGACCGATGGATATATGGTAGTGCCAGTTTTCTATAAGGTGGATCCATCTGTTGTTAGGTGGCAGATTAGGAGTTTTGCAGATGCATTTGCCAAACATGAAAAGCGCTTCAAGGAGGAGATCGGCAAGGTGGACAAGTGGAGAAAAGCTCTTAAAGATGTAGCCGATCTAGGAGGGATGGTCTTAGGAGATCAGTACGAATCACAGCTCATCCAAAATATTGTTGACGAGATTGAAAAAAAAATGGATCCCATTACATTAAATGTTGCTCCATATGCTGTTGGAATAAACGCTCGTGTGGAAGACCTCAACATGTGGTTAAGAGATGGATCAGTTGATGTTGGTGTGGCTGGCATCTACGGGATGGGTGGAATTGGCAAGACCACTATTGCTAAAGCTGCTTATAATCTGAATCGGGATAGATTTGAAGGAAGAAGTTTTCTTGCAGATGTTAGAGCAACTACAGAACAACCTAATGGTTTGGTTCGCTTGCAGAGGAACCTTCTTTCAGATATTCAAAGGAGAAAAACAAAAAAGATAAACAGCGTGGATGAAGGTACAAGCAAAATCAAGAATGTTGTATGCTGCAAAAGAGTACTTATTGTTCTTGATGATGTGAACGACTTGGACCAGTTCAATGCGATTCTTGGAATGCGAGAGTGGTTTCATCCAGGAAGCAAGATTATCATAACAACTAGAGATAAACACTTGTTAACGGCTACTGGGGTTTCTAACATGTTCATGGTTCCAGAATTGGATGAGCATGAATCACTTCAGCTTTTCAGTTGGCATGCCTTCAGACAAGCTCATCCTATCGAAGGCTACATGGAGGTTTCAGCACTCATAGTAAAACACTGTGGAGGGCTACCGTTAGCCCTCCAAGTTCTGGGGTCTTCTCTATTTGGAAAAAGTGTACATGTATGGTACAGTACATTAGAGAAGTTAGATGTGGTTCCTGATAGCAAAATTCAAAGAATTCTTAGAATAAGCTACGATTATCTACAAGATCATCATGACAAATCTTTATTCCTTCATATAGCATGTTTCTTCATAGGAAAGAAGAGAAACTTGACATTTACAGTACTGGACAACCTTGAGTTTTTCACAGCAAGTGGAGTTCAAAATCTAGTTGATAGATGTCTACTGCAAATTAATGACGGCAAGTTGATGATGCATCAGTTAATCAGAGACATGGGAAGAGGAATTGTTCGCGAAGAATCAGTTGAGGACCCTGGAAAACGTAGTAGAGTGGCGCAAAAAGATGCCTATGATATTTTGAAAAACGCATCAGGCACGCAAACTATCAAGGGTCTCTTGCTCAACCTTTCTATGCTAATGGATGGTATGTCTTCCAAGGCAATACTCAACAATAAACGAGGGCATGCCGAAGAATTAGATGGAAATTGTTCAAGACGGCGTCGACTTAGTTTCTTCTCCTGGCAATCAATCAACTATTCCTCAAAAAATTTAGCTTCTATATCGAGTGTGGCAGACTTCAAGACAGAGGCATTCAAAAAGATGCCCAATCTTCAACTTCTCCTGCTCAATAATGTAAAAGTCAGTGGTGGCTACGGAGAGTTTCCAAAAACACTAATATGGTTGTCTTGGCAAGGGTTCCCCATGAAATCAATACCAGCCGATTTTTGTTTGAAAAATCTAGTTGCTCTTGAGTTGAAGCATAGCATCCTGCAAGTTGTATGGAAGGGAACCAAGTTTTTTCCAGGATTGAAAATCCTTGATCTCAGTCATTCACAGGGTCTTGTTAGAACCCCAGACTTGTCCGGACTCCCTAACCTTGAGAGATTAATTCTTAAAGGTTGCATAAATATTGTCGAGATTGATGAATCCATCGGAGAACTTCAGAAACTTGTATTCTTGAATCTAAAAGACTGCAAAAATCTCGTGAAGCTTCCAAGAAGAATTAGCATGTTGAGATCTCTTCATGAGCTAATCCTTTCTGGCTGCTCAAAGCTCGCTGATAATGAGAAACTCGGTCTTTTGTCAAATAAACCATGGTTATCAATCTGTTCGTGGGTATCACCAAGAAAAAGTCTTGAAGCAACCAGCAGCTTCTCATTGGCATATTTGCCACAGTCTTTAGGAAGCTTAAGTCTGGCTGATTGCAATCTATCAAAGATTTCCGATGATATTAGTGTTCTGTCCTCGTTGAAGCATCTGGATATAAGTGGCAACCCAATGCTAAGTCTTCCGGAGAGCATAAAGACTCTTACTATGCTTGACACCCTTTTCTTAGGACATTGCAACAAGCTCAAAATGCTTCCAGAGCTCCCACCAAGTTTAAGCACTTTGCATGCAGAAGGTTGTACATCGTTGAGAAAAGTTACGAACTTACCAAACTATTTCATATCCCTACAACCATATCTTACGCATTGCGAAGAGTTAGCTGAAGTTCAAAGCTTATTCACTATAAAACCATTGAGAAGCACCGACATACAGATGATAAAATATATGGGTCTGTACAATTTGGAATCCACTGAAAGCATTGAAGTCAAAATGAGCAATCAGCTGACATTTACAGACAAGACAGGTCCACTCCAGGGACTCTATGAATGTGGAATATTTAGCATTTTCCTGCATGGAAGCACAATTCCTGACTGCTACACCTACAGAAGCATGGGTGATTCAGTGTTGTCTATAACTGTTCCTTCCCATCTTAAGCTTAAGATCCGAGGCCTAAATGTATGTATTCTATATGCACTAGGCCCATGCCACCACCTTAAAGTCAGTAATGACACGAAGGGTCTTATGTGGACATACTGCCCAGTCACTGCAGGTGTTCCAAAAGAGAATGAAGAAATGTTATGGCTAAGCCATTGGCAGTTTGAGAATGATGAGATAGAGTATATAATCTGCTCAACTGATCTCTACGTAGTTGGAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

971

Amino Acids

109.39

Weight (kDa)

8.51

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 1 - 89 9e-15 TIR domain
NB-ARC PF00931 103 - 272 7.4e-21 NB-ARC domain
WHD_ROQ1 PF23282 341 - 411 1.7e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 576 - 790 3.5e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 417
AarI CACCTGC 1 cut(s) 2795
Acc36I ACCTGC 1 cut(s) 2795
AccB7I CCANNNNNTGG 1 cut(s) 898
AccI GTMKAC 2 cut(s) 1153, 1432
AccII CGCG 1 cut(s) 1224
AccIII TCCGGA 2 cut(s) 1793, 2221
AclWI GGATC 6 cut(s) 50, 63, 254, 267, 349, 2692
AcsI RAATTY 3 cut(s) 978, 987, 1477
AcuI CTGAAG 4 cut(s) 770, 793, 1856, 2439
AcyI GRCGYC 1 cut(s) 1429
AfaI GTAC 9 cut(s) 209, 571, 609, 927, 938, 943, 1095, 2338, 2492
AfeI AGCGCT 1 cut(s) 119
AfiI CCNNNNNNNGG 3 cut(s) 830, 898, 2570
AflII CTTAAG 3 cut(s) 2119, 2687, 2693
AflIII ACRYGT 3 cut(s) 324, 747, 928
AjnI CCWGG 5 cut(s) 682, 1243, 1448, 1732, 2568
Alw21I GWGCWC 1 cut(s) 2301
Alw26I GTCTC 3 cut(s) 1194, 1328, 2904
AlwI GGATC 6 cut(s) 50, 63, 254, 267, 349, 2692
AlwNI CAGNNNCTG 1 cut(s) 2801
Aor13HI TCCGGA 2 cut(s) 1793, 2221
Aor51HI AGCGCT 1 cut(s) 119
AoxI GGCC 2 cut(s) 2704, 2734
ApeKI GCWGC 4 cut(s) 410, 597, 1974, 2079
ApoI RAATTY 3 cut(s) 978, 987, 1477
AseI ATTAAT 2 cut(s) 1164, 1817
Asp700I GAANNNNTTC 2 cut(s) 783, 1417
AspLEI GCGC 2 cut(s) 120, 1267
AspS9I GGNCC 5 cut(s) 15, 640, 1240, 2561, 2735
AsuHPI GGTGA 2 cut(s) 2043, 2663
AvaII GGWCC 4 cut(s) 15, 640, 1240, 2561
BamHI GGATCC 2 cut(s) 55, 259
BanII GRGCYC 1 cut(s) 2301
BauI CACGAG 2 cut(s) 306, 1913
BbsI GAAGAC 4 cut(s) 321, 897, 1353, 2210
Bbv12I GWGCWC 1 cut(s) 2301
BbvI GCAGC 4 cut(s) 397, 584, 1961, 2091
BccI CCATC 9 cut(s) 14, 67, 184, 332, 370, 1346, 1403, 1868, 2691
BceAI ACGGC 3 cut(s) 744, 1186, 1442
BcgI CGANNNNNNTGC 2 cut(s) 1894, 1928
BciT130I CCWGG 5 cut(s) 684, 1245, 1450, 1734, 2570
BcoDI GTCTC 3 cut(s) 1194, 1328, 2904
BfaI CTAG 5 cut(s) 182, 708, 1139, 1670, 2732
BfmI CTRYAG 3 cut(s) 474, 2638, 2802
BfoI RGCGCY 1 cut(s) 121
BfrI CTTAAG 3 cut(s) 2119, 2687, 2693
BfuAI ACCTGC 1 cut(s) 2795
BglII AGATCT 1 cut(s) 1946
BisI GCNGC 4 cut(s) 411, 598, 1975, 2080
BlpI GCTNAGC 1 cut(s) 2840
BlsI GCNGC 4 cut(s) 412, 599, 1976, 2081
BmcAI AGTACT 2 cut(s) 609, 1095
Bme1390I CCNGG 5 cut(s) 684, 1245, 1450, 1734, 2570
Bme18I GGWCC 4 cut(s) 15, 640, 1240, 2561
BmgT120I GGNCC 5 cut(s) 15, 640, 1240, 2561, 2735
BmiI GGNNCC 8 cut(s) 57, 261, 512, 759, 966, 1242, 1628, 1720
BmrFI CCNGG 5 cut(s) 684, 1245, 1450, 1734, 2570
BmrI ACTGGG 2 cut(s) 744, 2789
BmsI GCATC 9 cut(s) 85, 375, 1173, 1195, 1264, 1307, 1521, 1704, 2194
BmuI ACTGGG 2 cut(s) 744, 2789
BpiI GAAGAC 4 cut(s) 321, 897, 1353, 2210
BplI GAGNNNNNCTC 2 cut(s) 145, 177
BpmI CTGGAG 1 cut(s) 2552
Bpu1102I GCTNAGC 1 cut(s) 2840
BpuEI CTTGAG 3 cut(s) 1127, 1700, 1829
BsaAI YACGTR 1 cut(s) 2902
BsaBI GATNNNNATC 1 cut(s) 2136
BsaHI GRCGYC 1 cut(s) 1429
BsaI GGTCTC 1 cut(s) 1328
BsaJI CCNNGG 5 cut(s) 1243, 1365, 2024, 2569, 2701
BsaWI WCCGGW 2 cut(s) 1793, 2221
BsaXI ACNNNNNCTCC 2 cut(s) 177, 207
Bsc4I CCNNNNNNNGG 3 cut(s) 830, 898, 2570
Bse1I ACTGG 5 cut(s) 38, 643, 739, 1101, 2795
Bse3DI GCAATG 2 cut(s) 2269, 2401
Bse8I GATNNNNATC 1 cut(s) 2136
BseAI TCCGGA 2 cut(s) 1793, 2221
BseBI CCWGG 5 cut(s) 684, 1245, 1450, 1734, 2570
BseDI CCNNGG 5 cut(s) 1243, 1365, 2024, 2569, 2701
BseGI GGATG 9 cut(s) 195, 222, 381, 568, 679, 775, 820, 1357, 1695
BseJI GATNNNNATC 1 cut(s) 2136
BseLI CCNNNNNNNGG 3 cut(s) 830, 898, 2570
BseMI GCAATG 2 cut(s) 2269, 2401
BseMII CTCAG 1 cut(s) 1768
BseNI ACTGG 5 cut(s) 38, 643, 739, 1101, 2795
BseRI GAGGAG 1 cut(s) 143
BseXI GCAGC 4 cut(s) 397, 584, 1961, 2091
Bsh1236I CGCG 1 cut(s) 1224
BshFI GGCC 2 cut(s) 2706, 2736
BsiHKAI GWGCWC 1 cut(s) 2301
BsiSI CCGG 2 cut(s) 1794, 2222
BslFI GGGAC 1 cut(s) 2586
BslI CCNNNNNNNGG 3 cut(s) 830, 898, 2570
BsmAI GTCTC 3 cut(s) 1194, 1328, 2904
BsmFI GGGAC 1 cut(s) 2586
BsmI GAATGC 2 cut(s) 669, 1520
BsnI GGCC 2 cut(s) 2706, 2736
Bso31I GGTCTC 1 cut(s) 1328
Bsp1286I GDGCHC 1 cut(s) 2301
Bsp13I TCCGGA 2 cut(s) 1793, 2221
Bsp1407I TGTACA 3 cut(s) 925, 2336, 2490
Bsp1720I GCTNAGC 1 cut(s) 2840
Bsp19I CCATGG 1 cut(s) 2024
Bsp68I TCGCGA 1 cut(s) 1224
BspANI GGCC 2 cut(s) 2706, 2736
BspCNI CTCAG 1 cut(s) 1767
BspEI TCCGGA 2 cut(s) 1793, 2221
BspFNI CGCG 1 cut(s) 1224
BspHI TCATGA 2 cut(s) 1024, 1954
BspLI GGNNCC 8 cut(s) 57, 261, 512, 759, 966, 1242, 1628, 1720
BspMAI CTGCAG 1 cut(s) 2806
BspMI ACCTGC 1 cut(s) 2795
BspPI GGATC 6 cut(s) 50, 63, 254, 267, 349, 2692
BspTI CTTAAG 3 cut(s) 2119, 2687, 2693
BspTNI GGTCTC 1 cut(s) 1328
BsrDI GCAATG 2 cut(s) 2269, 2401
BsrGI TGTACA 3 cut(s) 925, 2336, 2490
BsrI ACTGG 5 cut(s) 38, 643, 739, 1101, 2795
BssECI CCNNGG 5 cut(s) 1243, 1365, 2024, 2569, 2701
BssNI GRCGYC 1 cut(s) 1429
BssSI CACGAG 2 cut(s) 306, 1913
BssT1I CCWWGG 2 cut(s) 1365, 2024
Bst2BI CACGAG 2 cut(s) 306, 1913
Bst2UI CCWGG 5 cut(s) 684, 1245, 1450, 1734, 2570
Bst4CI ACNGT 6 cut(s) 869, 882, 941, 1093, 2106, 2674
Bst6I CTCTTC 4 cut(s) 1067, 1204, 1956, 2403
BstACI GRCGYC 1 cut(s) 1429
BstAFI CTTAAG 3 cut(s) 2119, 2687, 2693
BstAUI TGTACA 3 cut(s) 925, 2336, 2490
BstBAI YACGTR 1 cut(s) 2902
BstC8I GCNNGC 7 cut(s) 364, 503, 804, 1307, 1395, 1986, 2325
BstDEI CTNAG 7 cut(s) 196, 992, 1436, 1754, 2213, 2263, 2840
BstDSI CCRYGG 1 cut(s) 2024
BstENI CCTNNNNNAGG 1 cut(s) 828
BstF5I GGATG 9 cut(s) 195, 222, 381, 568, 679, 775, 820, 1357, 1695
BstFNI CGCG 1 cut(s) 1224
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 2 cut(s) 120, 1267
BstMAI GTCTC 3 cut(s) 1194, 1328, 2904
BstMWI GCNNNNNNNGC 4 cut(s) 287, 410, 2287, 2296
BstNI CCWGG 5 cut(s) 684, 1245, 1450, 1734, 2570
BstNSI RCATGY 8 cut(s) 328, 751, 806, 932, 1056, 1397, 1942, 2327
BstSCI CCNGG 5 cut(s) 682, 1243, 1448, 1732, 2568
BstSFI CTRYAG 3 cut(s) 474, 2638, 2802
BstSNI TACGTA 1 cut(s) 2902
BstUI CGCG 1 cut(s) 1224
BstV1I GCAGC 4 cut(s) 397, 584, 1961, 2091
BstV2I GAAGAC 4 cut(s) 321, 897, 1353, 2210
BstX2I RGATCY 4 cut(s) 55, 259, 1946, 2697
BstYI RGATCY 4 cut(s) 55, 259, 1946, 2697
BsuRI GGCC 2 cut(s) 2706, 2736
BtgI CCRYGG 1 cut(s) 2024
BtsCI GGATG 9 cut(s) 195, 222, 381, 568, 679, 775, 820, 1357, 1695
BtsI GCAGTG 1 cut(s) 2799
BtsIMutI CAGTG 5 cut(s) 865, 1579, 2505, 2664, 2799
BtuMI TCGCGA 1 cut(s) 1224
BveI ACCTGC 1 cut(s) 2795
Cac8I GCNNGC 7 cut(s) 364, 503, 804, 1307, 1395, 1986, 2325
CaiI CAGNNNCTG 1 cut(s) 2801
CciI TCATGA 2 cut(s) 1024, 1954
CfoI GCGC 2 cut(s) 120, 1267
Cfr13I GGNCC 5 cut(s) 15, 640, 1240, 2561, 2735
CseI GACGC 1 cut(s) 1418
Csp6I GTAC 9 cut(s) 208, 570, 608, 926, 937, 942, 1094, 2337, 2491
CviQI GTAC 9 cut(s) 208, 570, 608, 926, 937, 942, 1094, 2337, 2491
DdeI CTNAG 7 cut(s) 196, 992, 1436, 1754, 2213, 2263, 2840
Eam1104I CTCTTC 4 cut(s) 1067, 1204, 1956, 2403
EarI CTCTTC 4 cut(s) 1067, 1204, 1956, 2403
Ecl136II GAGCTC 1 cut(s) 2299
Eco105I TACGTA 1 cut(s) 2902
Eco130I CCWWGG 2 cut(s) 1365, 2024
Eco147I AGGCCT 1 cut(s) 2706
Eco24I GRGCYC 1 cut(s) 2301
Eco31I GGTCTC 1 cut(s) 1328
Eco47I GGWCC 4 cut(s) 15, 640, 1240, 2561
Eco47III AGCGCT 1 cut(s) 119
Eco53kI GAGCTC 1 cut(s) 2299
Eco57I CTGAAG 4 cut(s) 770, 793, 1856, 2439
EcoICRI GAGCTC 1 cut(s) 2299
EcoNI CCTNNNNNAGG 1 cut(s) 828
EcoO109I RGGNCCY 1 cut(s) 1240
EcoRI GAATTC 1 cut(s) 987
EcoRII CCWGG 5 cut(s) 682, 1243, 1448, 1732, 2568
EcoT14I CCWWGG 2 cut(s) 1365, 2024
EcoT22I ATGCAT 2 cut(s) 100, 1188
EcoT38I GRGCYC 1 cut(s) 2301
ErhI CCWWGG 2 cut(s) 1365, 2024
FalI AAGNNNNNCTT 4 cut(s) 1064, 1096, 1310, 1342
FaqI GGGAC 1 cut(s) 2586
FauNDI CATATG 1 cut(s) 286
FblI GTMKAC 2 cut(s) 1153, 1432
Fnu4HI GCNGC 4 cut(s) 411, 598, 1975, 2080
FokI GGATG 9 cut(s) 202, 209, 388, 575, 666, 782, 807, 1364, 1682
FriOI GRGCYC 1 cut(s) 2301
Fsp4HI GCNGC 4 cut(s) 411, 598, 1975, 2080
FspBI CTAG 5 cut(s) 182, 708, 1139, 1670, 2732
GlaI GCGC 2 cut(s) 119, 1266
GluI GCNGC 4 cut(s) 411, 598, 1975, 2080
GsuI CTGGAG 1 cut(s) 2552
HaeII RGCGCY 1 cut(s) 121
HaeIII GGCC 2 cut(s) 2706, 2736
HapII CCGG 2 cut(s) 1794, 2222
HgaI GACGC 1 cut(s) 1418
HhaI GCGC 2 cut(s) 120, 1267
Hin1I GRCGYC 1 cut(s) 1429
Hin6I GCGC 2 cut(s) 118, 1265
HinP1I GCGC 2 cut(s) 118, 1265
HincII GTYRAC 3 cut(s) 238, 726, 1433
HindII GTYRAC 3 cut(s) 238, 726, 1433
HindIII AAGCTT 4 cut(s) 1919, 2116, 2428, 2690
HpaI GTTAAC 1 cut(s) 726
HpaII CCGG 2 cut(s) 1794, 2222
HphI GGTGA 2 cut(s) 2043, 2663
Hpy166II GTNNAC 9 cut(s) 145, 238, 631, 726, 926, 1154, 1433, 2564, 2784
Hpy188I TCNGA 8 cut(s) 423, 523, 812, 1199, 1865, 1875, 2155, 2702
Hpy8I GTNNAC 9 cut(s) 145, 238, 631, 726, 926, 1154, 1433, 2564, 2784
Hpy99I CGWCG 1 cut(s) 1434
HpyCH4III ACNGT 6 cut(s) 869, 882, 941, 1093, 2106, 2674
HpyCH4IV ACGT 3 cut(s) 7, 1252, 2901
HpyF10VI GCNNNNNNNGC 4 cut(s) 287, 410, 2287, 2296
HpyF3I CTNAG 7 cut(s) 196, 992, 1436, 1754, 2213, 2263, 2840
HpySE526I ACGT 3 cut(s) 7, 1252, 2901
Hsp92I GRCGYC 1 cut(s) 1429
HspAI GCGC 2 cut(s) 118, 1265
Kpn2I TCCGGA 2 cut(s) 1793, 2221
KspAI GTTAAC 1 cut(s) 726
LmnI GCTCC 2 cut(s) 286, 2304
Lsp1109I GCAGC 4 cut(s) 397, 584, 1961, 2091
LweI GCATC 9 cut(s) 85, 375, 1173, 1195, 1264, 1307, 1521, 1704, 2194
MaeI CTAG 5 cut(s) 182, 708, 1139, 1670, 2732
MaeII ACGT 3 cut(s) 7, 1252, 2901
MaeIII GTNAC 2 cut(s) 2353, 2797
MflI RGATCY 4 cut(s) 55, 259, 1946, 2697
MhlI GDGCHC 1 cut(s) 2301
MlyI GAGTC 3 cut(s) 1791, 2230, 2568
MmeI TCCRAC 2 cut(s) 274, 2887
Mph1103I ATGCAT 2 cut(s) 100, 1188
MroI TCCGGA 2 cut(s) 1793, 2221
MroXI GAANNNNTTC 2 cut(s) 783, 1417
MslI CAYNNNNRTG 3 cut(s) 752, 1606, 2468
MspA1I CMGCKG 1 cut(s) 2539
MspCI CTTAAG 3 cut(s) 2119, 2687, 2693
MspI CCGG 2 cut(s) 1794, 2222
MspR9I CCNGG 5 cut(s) 684, 1245, 1450, 1734, 2570
Mva1269I GAATGC 2 cut(s) 669, 1520
MvaI CCWGG 5 cut(s) 684, 1245, 1450, 1734, 2570
MvnI CGCG 1 cut(s) 1224
MwoI GCNNNNNNNGC 4 cut(s) 287, 410, 2287, 2296
NcoI CCATGG 1 cut(s) 2024
NdeI CATATG 1 cut(s) 286
NlaIV GGNNCC 8 cut(s) 57, 261, 512, 759, 966, 1242, 1628, 1720
NmuCI GTSAC 1 cut(s) 2797
NruI TCGCGA 1 cut(s) 1224
NsiI ATGCAT 2 cut(s) 100, 1188
NspI RCATGY 8 cut(s) 328, 751, 806, 932, 1056, 1397, 1942, 2327
PaeI GCATGC 3 cut(s) 806, 1397, 2327
PagI TCATGA 2 cut(s) 1024, 1954
PaqCI CACCTGC 1 cut(s) 2795
PceI AGGCCT 1 cut(s) 2706
PciI ACATGT 3 cut(s) 324, 747, 928
PctI GAATGC 2 cut(s) 669, 1520
PdmI GAANNNNTTC 2 cut(s) 783, 1417
PfeI GAWTC 9 cut(s) 212, 424, 655, 779, 1229, 1856, 1891, 2501, 2654
PflFI GACNNNGTC 1 cut(s) 1428
PflMI CCANNNNNTGG 1 cut(s) 898
PfoI TCCNGGA 2 cut(s) 682, 1732
PkrI GCNGC 4 cut(s) 412, 599, 1976, 2081
PleI GAGTC 3 cut(s) 1791, 2230, 2568
PpsI GAGTC 3 cut(s) 1791, 2230, 2568
Ppu21I YACGTR 1 cut(s) 2902
PpuMI RGGWCCY 1 cut(s) 1240
PscI ACATGT 3 cut(s) 324, 747, 928
PshBI ATTAAT 2 cut(s) 1164, 1817
PsiI TTATAA 1 cut(s) 417
Psp124BI GAGCTC 1 cut(s) 2301
Psp5II RGGWCCY 1 cut(s) 1240
Psp6I CCWGG 5 cut(s) 682, 1243, 1448, 1732, 2568
PspGI CCWGG 5 cut(s) 682, 1243, 1448, 1732, 2568
PspN4I GGNNCC 8 cut(s) 57, 261, 512, 759, 966, 1242, 1628, 1720
PspPI GGNCC 5 cut(s) 15, 640, 1240, 2561, 2735
PspPPI RGGWCCY 1 cut(s) 1240
PsrI GAACNNNNNNTAC 2 cut(s) 600, 632
PstI CTGCAG 1 cut(s) 2806
PstNI CAGNNNCTG 1 cut(s) 2801
PsuI RGATCY 4 cut(s) 55, 259, 1946, 2697
PsyI GACNNNGTC 1 cut(s) 1428
PvuII CAGCTG 1 cut(s) 2539
RruI TCGCGA 1 cut(s) 1224
RsaI GTAC 9 cut(s) 209, 571, 609, 927, 938, 943, 1095, 2338, 2492
RsaNI GTAC 9 cut(s) 208, 570, 608, 926, 937, 942, 1094, 2337, 2491
RseI CAYNNNNRTG 3 cut(s) 752, 1606, 2468
SacI GAGCTC 1 cut(s) 2301
SalI GTCGAC 1 cut(s) 1431
SatI GCNGC 4 cut(s) 411, 598, 1975, 2080
Sau96I GGNCC 5 cut(s) 15, 640, 1240, 2561, 2735
ScaI AGTACT 2 cut(s) 609, 1095
SchI GAGTC 3 cut(s) 1791, 2230, 2568
ScrFI CCNGG 5 cut(s) 684, 1245, 1450, 1734, 2570
SduI GDGCHC 1 cut(s) 2301
SfaNI GCATC 9 cut(s) 85, 375, 1173, 1195, 1264, 1307, 1521, 1704, 2194
SfcI CTRYAG 3 cut(s) 474, 2638, 2802
SinI GGWCC 4 cut(s) 15, 640, 1240, 2561
SmiMI CAYNNNNRTG 3 cut(s) 752, 1606, 2468
SmlI CTYRAG 6 cut(s) 1106, 1679, 1808, 2119, 2687, 2693
SmoI CTYRAG 6 cut(s) 1106, 1679, 1808, 2119, 2687, 2693
SnaBI TACGTA 1 cut(s) 2902
SphI GCATGC 3 cut(s) 806, 1397, 2327
SseBI AGGCCT 1 cut(s) 2706
SspI AATATT 3 cut(s) 232, 1840, 2592
SspMI CTAG 5 cut(s) 182, 708, 1139, 1670, 2732
SstI GAGCTC 1 cut(s) 2301
StuI AGGCCT 1 cut(s) 2706
StyD4I CCNGG 5 cut(s) 682, 1243, 1448, 1732, 2568
StyI CCWWGG 2 cut(s) 1365, 2024
TaaI ACNGT 6 cut(s) 869, 882, 941, 1093, 2106, 2674
TaiI ACGT 3 cut(s) 10, 1255, 2904
TaqI TCGA 4 cut(s) 828, 1432, 1493, 1845
TaqII GACCGA 2 cut(s) 32, 1994
TatI WGTACW 6 cut(s) 607, 925, 941, 1093, 2336, 2490
TfiI GAWTC 9 cut(s) 212, 424, 655, 779, 1229, 1856, 1891, 2501, 2654
TscAI CASTG 5 cut(s) 872, 1579, 2512, 2664, 2806
TseFI GTSAC 1 cut(s) 2797
TseI GCWGC 4 cut(s) 410, 597, 1974, 2079
Tsp45I GTSAC 1 cut(s) 2797
TspGWI ACGGA 1 cut(s) 1599
TspRI CASTG 5 cut(s) 872, 1579, 2512, 2664, 2806
Tth111I GACNNNGTC 1 cut(s) 1428
Van91I CCANNNNNTGG 1 cut(s) 898
Vha464I CTTAAG 3 cut(s) 2119, 2687, 2693
VpaK11BI GGWCC 4 cut(s) 15, 640, 1240, 2561
VspI ATTAAT 2 cut(s) 1164, 1817
XagI CCTNNNNNAGG 1 cut(s) 828
XapI RAATTY 3 cut(s) 978, 987, 1477
XceI RCATGY 8 cut(s) 328, 751, 806, 932, 1056, 1397, 1942, 2327
XmiI GTMKAC 2 cut(s) 1153, 1432
XmnI GAANNNNTTC 2 cut(s) 783, 1417
XspI CTAG 5 cut(s) 182, 708, 1139, 1670, 2732
ZrmI AGTACT 2 cut(s) 609, 1095
Zsp2I ATGCAT 2 cut(s) 100, 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.