Prupe.3G130900_v2.0.a1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000654
Physical Location & Seq
Reverse (-)
13377924 .. 13381571
3648 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G130900.1

Sequence Viewer

Length: 2958 bp
ATGGGTGGAGTTGGCAAAACCACTATTGCCAAAGCTGCTTATAAGCAGAACTTTGAAAAATTTCAAGGTAGCAGCTTTCTTCCAGATGTTAGAGCAGCGTCAGAACAACCTAATGGTCTGGTTTGCTTGCAAAGAATACTTCTTTCGGATATCCAAAAAGGGAAAACAAAGAAAATATACAGCATAGATGAAGGAATGAGCAAGATCAAACTTCTTGTGCGTTGCAAAAGGGTTCTTATAGTCCTTGATGATGTGAATCACTCTGAGCAATTCAATGCAATTCTTGGAATGCGAGAATGGTTTCATCCAGGAAGTAAAATTATCGTAACAACTAGACATGAAAATCTGTTAAATGATCATGCAGTTTATGCAATGTTTAAGGTTAAAGGATTAGGTGAGGGTGAATCACTGGAGCTTTTCAGTTGGCATGCCTTCAAACAAGCGCATCCTATCAAAGGTTACATGAACCTTTCAAGATCTGTAGTACAACACTGTGAAGGCCTTCCACTAGCTCTCCAAGTTTTGGGATCTTCTCTATTTGGAAAAAGTGTAGATCTATGGCAAAGTGCATTACAGAAGTTGCACGTGATTCCTGATGACAAGATTCAAAAGATTCTTAGAATAAGCTTTGATTCTCTAAAAGATGATCATGACAGGAATTTATTCCTTCATATTGTCTGTTTCTTCATAGAAAAGAAGATGGGTTATACAATTACAGTACTAGACAATTTAAATTTTTACACAAGGATTGGAATTCAAAATCTAGTTGATAGATGTCTAGTGGAAATTGATATAGACAACAGGTTGATCATGCATCAATTACTTAGAGACATGGGAAGGGCAATTATTCGTGAAGAATCACCCGAGGACCCTGGAAAGCGTAGTAGAGTGTGGCATAAAGATGCCTCTGATGTTTTGAGAAAATTAACTCAGGGAACAGAAACTATTAAGGGACTCATGCTCAACCTTCCTAGCGAGGCAATATTTAGTACAAGTAACCAAAACCGGTGCCATGTTGAAGATTTTGATGGAAATTGTTCAAGACGACGTCGACTTGGTTACTTCTCTTGGATATCAATTAACTCTTCCTCAACAAATTCAGCTGCTGCATCAAATGAGGTAGACTTCAAGGCAGAGGCATTTAGAAGAATGCACAATCTTGAACTGCTCCTGCTCGATAATGTAAAAGTCGGTGGAGATTATGAAGATTTTCCAAAAAAATTAATATGGTTGTGCTGGCGAGGATTCCCTTTAAAATCAATACCAGAAAAATTTTATTTGGAAAATCTGGTTGGTCTTGACCTACGAAATAGCACCCTACAACATGTTTGGAAGGGAACCAGGTTTCTTCCAGGACTGAAAATTCTTAATCTCAGTCATTCACATAGCCTTGTGACAACCCCCGACTTGTCAGGAGTCCCTAACCTTGAGAAATTGATTCTTAAAGACTGCATAAATTTGGTTGTGATTGATGAATCCCTAGGAAACCTGGAGAAACTCATCTTCTTGAATCTAAAAGACTGCAGAAGTCTCATGAAGCTTCCAACAAGAATTAGCATGTTGAGATCTCTTCAGGAACTTGATCTTTCTGGGTGCTCAAAGCTTGTGCTTCATACCAGTACGACTGCTGCTAATCACTTACACTCTACAACTAGAGTTAGGAAGAAATTAAATATGTTATCAGAAAAAATATGGCAATCGATATGGTTGTGGAGGTCATGGGTATCACCAAGAAATAAGCTTGAATCAGCCAGTTTGTCCATGGAAATCTGGCCAAATTGCTTAGGAACCTTAAGTCTGGCTGACTGCAATCTGTCAGAGATTCCTGGTGATCTTAGTATCTTGTCCTTGTTGAAGCATTTGAATCTAAGTAGAAACCCAATTCTGAGGCTACCAGAAAACATGAACGGTCTTATTATGCTCCAGACTCTTGAGATACAAGGTTGCACAAAGCTACGCACTCTTCCAAAGCTCCCACGGAGTCTAAGAAAATTGCATGCAAGTTATTGTACATCACTTGAAAGAATAACGAATCTACCAAACATGTTTGAATCATTGGATTCAAGTCTTTGGAAATGCAAGAAGTTACATGAAGTTCAAAGCTTGTTCAACATAAAACCATTGGGAAGGGTTGACATAGAAATGATCAGTGATATGGGCCTGTTTAATTTGGAATCCACAGGAGGAAGTACTGAAGTTGAAATGACCAACTACATGACATGTACAACAAGGAAGGGTCCCCTCCAGGCACTGTATGAATGTGGCATAATTAGTATTTTTGTTCAAGGAAACAAAATTCCAGATTGGTTCACCTACAGGAGCATGGGTAATTCTGTTTTGTCTATTATTCTACCTTCACATCTTAATCTCAAGATCCGAGGGTTAAATGTATGTGTTATGTATTCACGCCGCCCATTTTGGTTTAGTGCTACAAACTTTCTTAAAGTTAGTAATGAGACCAAAGGTCTTAAGTGGACCTATTGCCCAGTTGCAGCAGGTCTCCCAAAAAAGAACCAAGACATGCTATGGCTAAGCCACTGGCGGTTTGAAAATGATGAATTGGAGGAAGGAGAACAAGTGCATGTTTCGATAAATGAGGAATTTAGTTTCTGGGCAAAGGAGTTCTGTATCCAGCTTGTGTATGAGAAGGACCCATCAAAAAGTGAAAATATAACAATCCAACAGGAAACACCTCCTTCGAGCCAGATTGCTGCTGCTGGAAATGTATCAGCATCAGCATCCAAGTATCAATTCGGGGCGGGCAAATACTTTTTATCTAACCATCGGGCTCGTATTCATCAATGTCAATTCTCAAATAGCCAGATGAACGCAGCGTCTCTTGAATATTCATACTTATTTGATCAAGATGTTCATCCTCCACGAAGGATTGGCCTTCATCCAGCATTGTTCTTTGACCGTTGTAAGAGAAACATCAGCAATATTAATTGTCTCCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

986

Amino Acids

112.37

Weight (kDa)

9.0

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 42
AatII GACGTC 1 cut(s) 1051
Acc36I ACCTGC 1 cut(s) 2486
AccB1I GGYRCC 1 cut(s) 1008
AccB7I CCANNNNNTGG 1 cut(s) 523
AccI GTMKAC 2 cut(s) 1051, 1122
AciI CCGC 3 cut(s) 2410, 2542, 2759
AclWI GGATC 2 cut(s) 535, 2368
AcoI YGGCCR 1 cut(s) 1772
AcuI CTGAAG 2 cut(s) 1556, 2214
AcvI CACGTG 1 cut(s) 586
AcyI GRCGYC 1 cut(s) 1048
AfaI GTAC 7 cut(s) 486, 720, 991, 1621, 2011, 2191, 2224
AfiI CCNNNNNNNGG 2 cut(s) 455, 523
AflII CTTAAG 2 cut(s) 1792, 2468
AflIII ACRYGT 3 cut(s) 1324, 2043, 2219
AgeI ACCGGT 1 cut(s) 1005
AhdI GACNNNNNGTC 1 cut(s) 2463
AjnI CCWGG 7 cut(s) 307, 871, 1340, 1351, 1488, 1825, 2244
AjuI GAANNNNNNNTTGG 4 cut(s) 1275, 1307, 2735, 2767
Alw21I GWGCWC 1 cut(s) 1598
Alw26I GTCTC 6 cut(s) 822, 1535, 2450, 2504, 2841, 2954
AlwI GGATC 2 cut(s) 535, 2368
AlwNI CAGNNNCTG 2 cut(s) 1106, 2251
Ama87I CYCGRG 1 cut(s) 863
AoxI GGCC 4 cut(s) 499, 1772, 2158, 2890
AseI ATTAAT 2 cut(s) 1223, 2943
AsiGI ACCGGT 1 cut(s) 1005
Asp700I GAANNNNTTC 6 cut(s) 60, 300, 501, 662, 1036, 1209
AspA2I CCTAGG 1 cut(s) 1480
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 5 cut(s) 868, 2158, 2237, 2475, 2650
AsuHPI GGTGA 6 cut(s) 407, 413, 852, 1719, 1841, 2302
AvaI CYCGRG 1 cut(s) 863
AvaII GGWCC 4 cut(s) 868, 2237, 2475, 2650
AvrII CCTAGG 1 cut(s) 1480
BalI TGGCCA 1 cut(s) 1774
BanI GGYRCC 1 cut(s) 1008
BanII GRGCYC 1 cut(s) 2791
BbrPI CACGTG 1 cut(s) 586
Bbv12I GWGCWC 1 cut(s) 1598
BccI CCATC 4 cut(s) 694, 1022, 2662, 2790
BciT130I CCWGG 7 cut(s) 309, 873, 1342, 1353, 1490, 1827, 2246
BciVI GTATCC 1 cut(s) 2639
BclI TGATCA 5 cut(s) 355, 646, 807, 2145, 2860
BcoDI GTCTC 6 cut(s) 822, 1535, 2450, 2504, 2841, 2954
BfaI CTAG 8 cut(s) 333, 509, 722, 764, 779, 972, 1481, 1653
BfmI CTRYAG 3 cut(s) 480, 1522, 2314
BfrI CTTAAG 2 cut(s) 1792, 2468
BfuAI ACCTGC 1 cut(s) 2486
BfuI GTATCC 1 cut(s) 2639
BglII AGATCT 3 cut(s) 476, 553, 1565
BlnI CCTAGG 1 cut(s) 1480
BlpI GCTNAGC 1 cut(s) 2531
BmcAI AGTACT 2 cut(s) 720, 2191
Bme1390I CCNGG 7 cut(s) 309, 873, 1342, 1353, 1490, 1827, 2246
Bme18I GGWCC 4 cut(s) 868, 2237, 2475, 2650
BmeRI GACNNNNNGTC 1 cut(s) 2463
BmeT110I CYCGRG 1 cut(s) 863
BmgT120I GGNCC 5 cut(s) 868, 2158, 2237, 2475, 2650
BmiI GGNNCC 7 cut(s) 870, 1010, 1339, 1789, 2238, 2239, 2652
BmrFI CCNGG 7 cut(s) 309, 873, 1342, 1353, 1490, 1827, 2246
BmrI ACTGGG 1 cut(s) 2480
BmsI GCATC 6 cut(s) 454, 823, 892, 1118, 2741, 2747
BmuI ACTGGG 1 cut(s) 2480
BpmI CTGGAG 5 cut(s) 431, 1511, 1907, 2228, 2936
Bpu10I CCTNAGC 1 cut(s) 1783
Bpu1102I GCTNAGC 1 cut(s) 2531
BpuEI CTTGAG 3 cut(s) 1448, 1952, 2354
Bsa29I ATCGAT 1 cut(s) 1700
BsaAI YACGTR 1 cut(s) 586
BsaBI GATNNNNATC 2 cut(s) 255, 2871
BsaHI GRCGYC 1 cut(s) 1048
BsaI GGTCTC 2 cut(s) 2450, 2504
BsaJI CCNNGG 6 cut(s) 864, 871, 1480, 1761, 1976, 2377
BsaWI WCCGGW 1 cut(s) 1005
BsaXI ACNNNNNCTCC 6 cut(s) 498, 528, 2311, 2341, 2562, 2592
Bsc4I CCNNNNNNNGG 2 cut(s) 455, 523
Bse118I RCCGGY 1 cut(s) 1005
Bse1I ACTGG 6 cut(s) 414, 1617, 1752, 2486, 2543, 2953
Bse3DI GCAATG 1 cut(s) 378
Bse8I GATNNNNATC 2 cut(s) 255, 2871
BseBI CCWGG 7 cut(s) 309, 873, 1342, 1353, 1490, 1827, 2246
BseCI ATCGAT 1 cut(s) 1700
BseDI CCNNGG 6 cut(s) 864, 871, 1480, 1761, 1976, 2377
BseGI GGATG 5 cut(s) 304, 445, 2738, 2872, 2896
BseJI GATNNNNATC 2 cut(s) 255, 2871
BseLI CCNNNNNNNGG 2 cut(s) 455, 523
BseMI GCAATG 1 cut(s) 378
BseMII CTCAG 4 cut(s) 255, 944, 1387, 1877
BseNI ACTGG 6 cut(s) 414, 1617, 1752, 2486, 2543, 2953
BshFI GGCC 4 cut(s) 501, 1774, 2160, 2892
BshNI GGYRCC 1 cut(s) 1008
BshTI ACCGGT 1 cut(s) 1005
BshVI ATCGAT 1 cut(s) 1700
BsiHKAI GWGCWC 1 cut(s) 1598
BsiHKCI CYCGRG 1 cut(s) 863
BsiSI CCGG 1 cut(s) 1006
BslFI GGGAC 3 cut(s) 966, 1403, 2223
BslI CCNNNNNNNGG 2 cut(s) 455, 523
BsmAI GTCTC 6 cut(s) 822, 1535, 2450, 2504, 2841, 2954
BsmBI CGTCTC 1 cut(s) 2841
BsmFI GGGAC 3 cut(s) 966, 1403, 2223
BsmI GAATGC 2 cut(s) 294, 1155
BsnI GGCC 4 cut(s) 501, 1774, 2160, 2892
Bso31I GGTCTC 2 cut(s) 2450, 2504
BsoBI CYCGRG 1 cut(s) 863
Bsp1286I GDGCHC 2 cut(s) 1598, 2791
Bsp1407I TGTACA 2 cut(s) 2009, 2222
Bsp1720I GCTNAGC 1 cut(s) 2531
Bsp19I CCATGG 1 cut(s) 1761
BspACI CCGC 3 cut(s) 2410, 2542, 2759
BspANI GGCC 4 cut(s) 501, 1774, 2160, 2892
BspCNI CTCAG 4 cut(s) 256, 943, 1386, 1878
BspDI ATCGAT 1 cut(s) 1700
BspHI TCATGA 2 cut(s) 649, 1533
BspLI GGNNCC 7 cut(s) 870, 1010, 1339, 1789, 2238, 2239, 2652
BspMAI CTGCAG 1 cut(s) 1526
BspMI ACCTGC 1 cut(s) 2486
BspPI GGATC 2 cut(s) 535, 2368
BspT107I GGYRCC 1 cut(s) 1008
BspTI CTTAAG 2 cut(s) 1792, 2468
BspTNI GGTCTC 2 cut(s) 2450, 2504
BsrDI GCAATG 1 cut(s) 378
BsrFI RCCGGY 1 cut(s) 1005
BsrGI TGTACA 2 cut(s) 2009, 2222
BsrI ACTGG 6 cut(s) 414, 1617, 1752, 2486, 2543, 2953
BssAI RCCGGY 1 cut(s) 1005
BssECI CCNNGG 6 cut(s) 864, 871, 1480, 1761, 1976, 2377
BssNI GRCGYC 1 cut(s) 1048
BssT1I CCWWGG 2 cut(s) 1480, 1761
Bst2UI CCWGG 7 cut(s) 309, 873, 1342, 1353, 1490, 1827, 2246
Bst4CI ACNGT 5 cut(s) 494, 718, 1910, 2253, 2918
Bst6I CTCTTC 3 cut(s) 1090, 1575, 1968
BstACI GRCGYC 1 cut(s) 1048
BstAFI CTTAAG 2 cut(s) 1792, 2468
BstAPI GCANNNNNTGC 1 cut(s) 368
BstAUI TGTACA 2 cut(s) 2009, 2222
BstBAI YACGTR 1 cut(s) 586
BstC8I GCNNGC 5 cut(s) 128, 429, 1238, 1998, 2761
BstDSI CCRYGG 2 cut(s) 1761, 1976
BstENI CCTNNNNNAGG 1 cut(s) 453
BstF5I GGATG 5 cut(s) 304, 445, 2738, 2872, 2896
BstHHI GCGC 1 cut(s) 445
BstMAI GTCTC 6 cut(s) 822, 1535, 2450, 2504, 2841, 2954
BstMWI GCNNNNNNNGC 2 cut(s) 35, 368
BstNI CCWGG 7 cut(s) 309, 873, 1342, 1353, 1490, 1827, 2246
BstNSI RCATGY 8 cut(s) 431, 1328, 1561, 2000, 2047, 2223, 2524, 2585
BstSCI CCNGG 7 cut(s) 307, 871, 1340, 1351, 1488, 1825, 2244
BstSFI CTRYAG 3 cut(s) 480, 1522, 2314
BstX2I RGATCY 5 cut(s) 476, 527, 553, 1565, 2373
BstYI RGATCY 5 cut(s) 476, 527, 553, 1565, 2373
Bsu15I ATCGAT 1 cut(s) 1700
BsuI GTATCC 1 cut(s) 2639
BsuRI GGCC 4 cut(s) 501, 1774, 2160, 2892
BsuTUI ATCGAT 1 cut(s) 1700
BtgI CCRYGG 2 cut(s) 1761, 1976
BtsCI GGATG 5 cut(s) 304, 445, 2738, 2872, 2896
BtsIMutI CAGTG 5 cut(s) 407, 490, 2155, 2249, 2536
BveI ACCTGC 1 cut(s) 2486
Cac8I GCNNGC 5 cut(s) 128, 429, 1238, 1998, 2761
CaiI CAGNNNCTG 2 cut(s) 1106, 2251
CciI TCATGA 2 cut(s) 649, 1533
CfoI GCGC 1 cut(s) 445
Cfr10I RCCGGY 1 cut(s) 1005
Cfr13I GGNCC 5 cut(s) 868, 2158, 2237, 2475, 2650
ClaI ATCGAT 1 cut(s) 1700
CseI GACGC 2 cut(s) 87, 2823
CsiI ACCWGGT 1 cut(s) 1340
Csp6I GTAC 7 cut(s) 485, 719, 990, 1620, 2010, 2190, 2223
CspAI ACCGGT 1 cut(s) 1005
CviQI GTAC 7 cut(s) 485, 719, 990, 1620, 2010, 2190, 2223
DraI TTTAAA 2 cut(s) 732, 1254
DriI GACNNNNNGTC 1 cut(s) 2463
EaeI YGGCCR 1 cut(s) 1772
Eam1104I CTCTTC 3 cut(s) 1090, 1575, 1968
Eam1105I GACNNNNNGTC 1 cut(s) 2463
EarI CTCTTC 3 cut(s) 1090, 1575, 1968
Eco130I CCWWGG 2 cut(s) 1480, 1761
Eco147I AGGCCT 1 cut(s) 501
Eco24I GRGCYC 1 cut(s) 2791
Eco31I GGTCTC 2 cut(s) 2450, 2504
Eco32I GATATC 2 cut(s) 151, 1074
Eco47I GGWCC 4 cut(s) 868, 2237, 2475, 2650
Eco57I CTGAAG 2 cut(s) 1556, 2214
Eco72I CACGTG 1 cut(s) 586
Eco88I CYCGRG 1 cut(s) 863
EcoNI CCTNNNNNAGG 1 cut(s) 453
EcoO109I RGGNCCY 3 cut(s) 868, 2237, 2650
EcoRI GAATTC 1 cut(s) 753
EcoRII CCWGG 7 cut(s) 307, 871, 1340, 1351, 1488, 1825, 2244
EcoRV GATATC 2 cut(s) 151, 1074
EcoT14I CCWWGG 2 cut(s) 1480, 1761
EcoT22I ATGCAT 1 cut(s) 816
EcoT38I GRGCYC 1 cut(s) 2791
ErhI CCWWGG 2 cut(s) 1480, 1761
Esp3I CGTCTC 1 cut(s) 2841
FalI AAGNNNNNCTT 2 cut(s) 35, 67
FaqI GGGAC 3 cut(s) 966, 1403, 2223
FauI CCCGC 1 cut(s) 2752
FbaI TGATCA 5 cut(s) 355, 646, 807, 2145, 2860
FblI GTMKAC 2 cut(s) 1051, 1122
FokI GGATG 5 cut(s) 291, 432, 2725, 2859, 2883
FriOI GRGCYC 1 cut(s) 2791
FspBI CTAG 8 cut(s) 333, 509, 722, 764, 779, 972, 1481, 1653
GlaI GCGC 1 cut(s) 444
GsuI CTGGAG 5 cut(s) 431, 1511, 1907, 2228, 2936
HaeIII GGCC 4 cut(s) 501, 1774, 2160, 2892
HapII CCGG 1 cut(s) 1006
HgaI GACGC 2 cut(s) 87, 2823
HhaI GCGC 1 cut(s) 445
Hin1I GRCGYC 1 cut(s) 1048
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HincII GTYRAC 2 cut(s) 1052, 2134
HindII GTYRAC 2 cut(s) 1052, 2134
HindIII AAGCTT 5 cut(s) 625, 1538, 1601, 1739, 2101
HpaII CCGG 1 cut(s) 1006
HphI GGTGA 6 cut(s) 407, 413, 852, 1719, 1841, 2302
Hpy166II GTNNAC 5 cut(s) 1052, 1123, 2134, 2310, 2475
Hpy188I TCNGA 8 cut(s) 103, 148, 265, 910, 1684, 1819, 1887, 2378
Hpy8I GTNNAC 5 cut(s) 1052, 1123, 2134, 2310, 2475
Hpy99I CGWCG 2 cut(s) 1050, 1053
HpyCH4III ACNGT 5 cut(s) 494, 718, 1910, 2253, 2918
HpyCH4IV ACGT 2 cut(s) 585, 1048
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 368
HpySE526I ACGT 2 cut(s) 585, 1048
Hsp92I GRCGYC 1 cut(s) 1048
HspAI GCGC 1 cut(s) 443
KflI GGGWCCC 1 cut(s) 2237
Ksp22I TGATCA 5 cut(s) 355, 646, 807, 2145, 2860
LmnI GCTCC 5 cut(s) 412, 1173, 1926, 1977, 2319
LweI GCATC 6 cut(s) 454, 823, 892, 1118, 2741, 2747
MabI ACCWGGT 1 cut(s) 1340
MaeI CTAG 8 cut(s) 333, 509, 722, 764, 779, 972, 1481, 1653
MaeII ACGT 2 cut(s) 585, 1048
MaeIII GTNAC 6 cut(s) 325, 458, 995, 1058, 1393, 2085
MflI RGATCY 5 cut(s) 476, 527, 553, 1565, 2373
MhlI GDGCHC 2 cut(s) 1598, 2791
MlsI TGGCCA 1 cut(s) 1774
MluNI TGGCCA 1 cut(s) 1774
MlyI GAGTC 4 cut(s) 948, 1425, 1921, 1990
MmeI TCCRAC 2 cut(s) 1568, 2704
Mox20I TGGCCA 1 cut(s) 1774
Mph1103I ATGCAT 1 cut(s) 816
MroXI GAANNNNTTC 6 cut(s) 60, 300, 501, 662, 1036, 1209
MscI TGGCCA 1 cut(s) 1774
MslI CAYNNNNRTG 2 cut(s) 900, 2141
Msp20I TGGCCA 1 cut(s) 1774
MspA1I CMGCKG 1 cut(s) 1103
MspCI CTTAAG 2 cut(s) 1792, 2468
MspI CCGG 1 cut(s) 1006
MspR9I CCNGG 7 cut(s) 309, 873, 1342, 1353, 1490, 1827, 2246
Mva1269I GAATGC 2 cut(s) 294, 1155
MvaI CCWGG 7 cut(s) 309, 873, 1342, 1353, 1490, 1827, 2246
MwoI GCNNNNNNNGC 2 cut(s) 35, 368
NcoI CCATGG 1 cut(s) 1761
NlaIV GGNNCC 7 cut(s) 870, 1010, 1339, 1789, 2238, 2239, 2652
NmuCI GTSAC 1 cut(s) 1393
NsiI ATGCAT 1 cut(s) 816
NspI RCATGY 8 cut(s) 431, 1328, 1561, 2000, 2047, 2223, 2524, 2585
PaeI GCATGC 2 cut(s) 431, 2000
PagI TCATGA 2 cut(s) 649, 1533
PceI AGGCCT 1 cut(s) 501
PciI ACATGT 3 cut(s) 1324, 2043, 2219
PctI GAATGC 2 cut(s) 294, 1155
PdmI GAANNNNTTC 6 cut(s) 60, 300, 501, 662, 1036, 1209
PflFI GACNNNGTC 1 cut(s) 1047
PflMI CCANNNNNTGG 1 cut(s) 523
PfoI TCCNGGA 2 cut(s) 307, 1351
PinAI ACCGGT 1 cut(s) 1005
PleI GAGTC 4 cut(s) 948, 1424, 1921, 1989
PmaCI CACGTG 1 cut(s) 586
PmlI CACGTG 1 cut(s) 586
PpsI GAGTC 4 cut(s) 948, 1424, 1921, 1989
Ppu21I YACGTR 1 cut(s) 586
PpuMI RGGWCCY 3 cut(s) 868, 2237, 2650
PscI ACATGT 3 cut(s) 1324, 2043, 2219
PshBI ATTAAT 2 cut(s) 1223, 2943
PsiI TTATAA 1 cut(s) 42
Psp5II RGGWCCY 3 cut(s) 868, 2237, 2650
Psp6I CCWGG 7 cut(s) 307, 871, 1340, 1351, 1488, 1825, 2244
PspCI CACGTG 1 cut(s) 586
PspGI CCWGG 7 cut(s) 307, 871, 1340, 1351, 1488, 1825, 2244
PspN4I GGNNCC 7 cut(s) 870, 1010, 1339, 1789, 2238, 2239, 2652
PspPI GGNCC 5 cut(s) 868, 2158, 2237, 2475, 2650
PspPPI RGGWCCY 3 cut(s) 868, 2237, 2650
PstI CTGCAG 1 cut(s) 1526
PstNI CAGNNNCTG 2 cut(s) 1106, 2251
PsuI RGATCY 5 cut(s) 476, 527, 553, 1565, 2373
PsyI GACNNNGTC 1 cut(s) 1047
PvuII CAGCTG 1 cut(s) 1103
RsaI GTAC 7 cut(s) 486, 720, 991, 1621, 2011, 2191, 2224
RsaNI GTAC 7 cut(s) 485, 719, 990, 1620, 2010, 2190, 2223
RseI CAYNNNNRTG 2 cut(s) 900, 2141
SalI GTCGAC 1 cut(s) 1050
Sau96I GGNCC 5 cut(s) 868, 2158, 2237, 2475, 2650
ScaI AGTACT 2 cut(s) 720, 2191
SchI GAGTC 4 cut(s) 948, 1425, 1921, 1990
ScrFI CCNGG 7 cut(s) 309, 873, 1342, 1353, 1490, 1827, 2246
SduI GDGCHC 2 cut(s) 1598, 2791
SexAI ACCWGGT 1 cut(s) 1340
SfaNI GCATC 6 cut(s) 454, 823, 892, 1118, 2741, 2747
SfcI CTRYAG 3 cut(s) 480, 1522, 2314
SinI GGWCC 4 cut(s) 868, 2237, 2475, 2650
SmiI ATTTAAAT 1 cut(s) 732
SmiMI CAYNNNNRTG 2 cut(s) 900, 2141
SmlI CTYRAG 5 cut(s) 1427, 1792, 1931, 2369, 2468
SmoI CTYRAG 5 cut(s) 1427, 1792, 1931, 2369, 2468
SphI GCATGC 2 cut(s) 431, 2000
SseBI AGGCCT 1 cut(s) 501
SsiI CCGC 3 cut(s) 2410, 2542, 2759
SspI AATATT 3 cut(s) 984, 2846, 2941
SspMI CTAG 8 cut(s) 333, 509, 722, 764, 779, 972, 1481, 1653
StuI AGGCCT 1 cut(s) 501
StyD4I CCNGG 7 cut(s) 307, 871, 1340, 1351, 1488, 1825, 2244
StyI CCWWGG 2 cut(s) 1480, 1761
SwaI ATTTAAAT 1 cut(s) 732
TaaI ACNGT 5 cut(s) 494, 718, 1910, 2253, 2918
TaiI ACGT 2 cut(s) 588, 1051
TaqI TCGA 5 cut(s) 1051, 1176, 1700, 2588, 2699
TatI WGTACW 6 cut(s) 484, 718, 989, 2009, 2189, 2222
TauI GCSGC 1 cut(s) 2412
TscAI CASTG 5 cut(s) 414, 497, 2155, 2256, 2543
TseFI GTSAC 1 cut(s) 1393
Tsp45I GTSAC 1 cut(s) 1393
TspGWI ACGGA 1 cut(s) 1993
TspRI CASTG 5 cut(s) 414, 497, 2155, 2256, 2543
Tth111I GACNNNGTC 1 cut(s) 1047
Van91I CCANNNNNTGG 1 cut(s) 523
Vha464I CTTAAG 2 cut(s) 1792, 2468
VpaK11BI GGWCC 4 cut(s) 868, 2237, 2475, 2650
VspI ATTAAT 2 cut(s) 1223, 2943
XagI CCTNNNNNAGG 1 cut(s) 453
XceI RCATGY 8 cut(s) 431, 1328, 1561, 2000, 2047, 2223, 2524, 2585
XcmI CCANNNNNNNNNTGG 1 cut(s) 1759
XmaJI CCTAGG 1 cut(s) 1480
XmiI GTMKAC 2 cut(s) 1051, 1122
XmnI GAANNNNTTC 6 cut(s) 60, 300, 501, 662, 1036, 1209
XspI CTAG 8 cut(s) 333, 509, 722, 764, 779, 972, 1481, 1653
ZraI GACGTC 1 cut(s) 1049
ZrmI AGTACT 2 cut(s) 720, 2191
Zsp2I ATGCAT 1 cut(s) 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.