FvH4_2g20700

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000654
Physical Location & Seq
Forward (+)
17305579 .. 17307251
1673 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g20700.t1

Sequence Viewer

Length: 681 bp
ATGAATGTGGTATATTCAGCATTTTCCTTCATGGAAACAAGATTCCTAATTGGTACAAGTACCGCCATGTCTATCATTGTTCCTTCCCATCCTAAACTCAAGATCACAGGCTTAAATGTCTGTGTCGTATACACAAGACGTGGAAAAATTGAGGATATTTGCACCTCACCGATTGTAAAAGCCAGTAACGAAGCGAAAGGTGTTATGTGGTCGTATTGCCCTGTCAGTGTAGGTCTTCCAAAAGAGAATGAAAAGATGTCATGGCTAAGCCATTGGCAGTTTGAGAATGACGAGTTAGAGTGTGGGGAAGAACTACGTGTTTCTGTGGATACCGACTACATGTATGTTCCGATCTTGATAAAGGAGCTTGCAGTCCAACTTGTGTACGAGCATAAGCATGAGCATGATGAAAATGAGGGCAAGGCATGTAATGGTATGCAATTATCAGATCATGATGTAGGTGTTGCAACTCAACACGAGGCACCTTATTGGAGCCAGGGTGTTCTCATCGGACATGTATCGGCACCGAAGTATCAAATTTGGCCGGGCACATACTTCCTTCGCAATCATCACAATGTAGTTTATCATATTCCAGTTGAAAGACCCGCCGGAAATATGCGTATATTTGAGCACCATCGTTTTGATGAGATGCGTGTCTTGGGACAAAAGAATCTTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

227

Amino Acids

25.92

Weight (kDa)

6.1

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 481, 523
AccI GTMKAC 1 cut(s) 129
AciI CCGC 2 cut(s) 63, 606
AcoI YGGCCR 1 cut(s) 542
AcsI RAATTY 1 cut(s) 537
AfaI GTAC 3 cut(s) 55, 61, 386
AflIII ACRYGT 3 cut(s) 316, 339, 514
AgsI TTSAA 1 cut(s) 599
AjiI CACGTC 1 cut(s) 140
AjnI CCWGG 1 cut(s) 495
AluBI AGCT 1 cut(s) 367
AluI AGCT 1 cut(s) 367
Alw21I GWGCWC 1 cut(s) 633
AoxI GGCC 1 cut(s) 542
ApoI RAATTY 1 cut(s) 537
AsuC2I CCSGG 1 cut(s) 546
AsuHPI GGTGA 1 cut(s) 159
BaeGI GKGCMC 1 cut(s) 551
BaeI ACNNNNGTAYC 2 cut(s) 515, 548
BanI GGYRCC 2 cut(s) 481, 523
BauI CACGAG 1 cut(s) 476
BbsI GAAGAC 1 cut(s) 227
Bbv12I GWGCWC 1 cut(s) 633
BccI CCATC 2 cut(s) 96, 642
BciT130I CCWGG 1 cut(s) 497
BciVI GTATCC 1 cut(s) 322
BcnI CCSGG 1 cut(s) 546
BfuI GTATCC 1 cut(s) 322
BlpI GCTNAGC 1 cut(s) 266
Bme1390I CCNGG 2 cut(s) 497, 546
BmgBI CACGTC 1 cut(s) 140
BmiI GGNNCC 3 cut(s) 483, 494, 525
BmrFI CCNGG 2 cut(s) 497, 546
BmsI GCATC 1 cut(s) 639
BpiI GAAGAC 1 cut(s) 227
Bpu1102I GCTNAGC 1 cut(s) 266
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 546
BsaAI YACGTR 1 cut(s) 317
BsaJI CCNNGG 1 cut(s) 496
BsaXI ACNNNNNCTCC 2 cut(s) 484, 514
Bse1I ACTGG 2 cut(s) 183, 593
BseBI CCWGG 1 cut(s) 497
BseDI CCNNGG 1 cut(s) 496
BseGI GGATG 1 cut(s) 88
BseNI ACTGG 2 cut(s) 183, 593
BseSI GKGCMC 1 cut(s) 551
BshFI GGCC 1 cut(s) 544
BshNI GGYRCC 2 cut(s) 481, 523
BsiHKAI GWGCWC 1 cut(s) 633
BsiSI CCGG 2 cut(s) 545, 609
BslFI GGGAC 1 cut(s) 675
BsmFI GGGAC 1 cut(s) 675
BsnI GGCC 1 cut(s) 544
Bsp1286I GDGCHC 2 cut(s) 551, 633
Bsp143I GATC 3 cut(s) 102, 351, 448
Bsp1720I GCTNAGC 1 cut(s) 266
BspACI CCGC 2 cut(s) 63, 606
BspANI GGCC 1 cut(s) 544
BspHI TCATGA 1 cut(s) 451
BspLI GGNNCC 3 cut(s) 483, 494, 525
BspT107I GGYRCC 2 cut(s) 481, 523
BsrI ACTGG 2 cut(s) 183, 593
BssECI CCNNGG 1 cut(s) 496
BssMI GATC 3 cut(s) 102, 351, 448
BssNAI GTATAC 1 cut(s) 130
BssSI CACGAG 1 cut(s) 476
Bst1107I GTATAC 1 cut(s) 130
Bst2BI CACGAG 1 cut(s) 476
Bst2UI CCWGG 1 cut(s) 497
BstBAI YACGTR 1 cut(s) 317
BstC8I GCNNGC 1 cut(s) 369
BstDEI CTNAG 1 cut(s) 266
BstF5I GGATG 1 cut(s) 88
BstKTI GATC 3 cut(s) 105, 354, 451
BstMBI GATC 3 cut(s) 102, 351, 448
BstNI CCWGG 1 cut(s) 497
BstNSI RCATGY 3 cut(s) 343, 429, 518
BstSCI CCNGG 2 cut(s) 495, 544
BstSLI GKGCMC 1 cut(s) 551
BstV2I GAAGAC 1 cut(s) 227
BstZ17I GTATAC 1 cut(s) 130
BsuI GTATCC 1 cut(s) 322
BsuRI GGCC 1 cut(s) 544
BtrI CACGTC 1 cut(s) 140
BtsCI GGATG 1 cut(s) 88
BtsIMutI CAGTG 1 cut(s) 232
Cac8I GCNNGC 1 cut(s) 369
CciI TCATGA 1 cut(s) 451
Csp6I GTAC 3 cut(s) 54, 60, 385
CviAII CATG 9 cut(s) 31, 67, 261, 340, 398, 404, 426, 452, 515
CviJI RGCY 7 cut(s) 111, 182, 265, 270, 367, 495, 544
CviKI_1 RGCY 7 cut(s) 111, 182, 265, 270, 367, 495, 544
CviQI GTAC 3 cut(s) 54, 60, 385
DdeI CTNAG 1 cut(s) 266
DpnI GATC 3 cut(s) 104, 353, 450
DpnII GATC 3 cut(s) 102, 351, 448
EaeI YGGCCR 1 cut(s) 542
EcoRII CCWGG 1 cut(s) 495
FaeI CATG 9 cut(s) 34, 70, 264, 343, 401, 407, 429, 455, 518
FaqI GGGAC 1 cut(s) 675
FatI CATG 9 cut(s) 30, 66, 260, 339, 397, 403, 425, 451, 514
FauI CCCGC 1 cut(s) 613
FblI GTMKAC 1 cut(s) 129
FokI GGATG 1 cut(s) 75
HaeIII GGCC 1 cut(s) 544
HapII CCGG 2 cut(s) 545, 609
Hin1II CATG 9 cut(s) 34, 70, 264, 343, 401, 407, 429, 455, 518
HinfI GANTC 2 cut(s) 42, 670
HpaII CCGG 2 cut(s) 545, 609
HphI GGTGA 1 cut(s) 159
Hpy166II GTNNAC 2 cut(s) 130, 385
Hpy188I TCNGA 3 cut(s) 351, 448, 512
Hpy188III TCNNGA 3 cut(s) 100, 355, 452
Hpy8I GTNNAC 2 cut(s) 130, 385
HpyAV CCTTC 3 cut(s) 37, 93, 569
HpyCH4IV ACGT 2 cut(s) 139, 316
HpyCH4V TGCA 4 cut(s) 162, 371, 439, 467
HpyF3I CTNAG 1 cut(s) 266
HpySE526I ACGT 2 cut(s) 139, 316
Hsp92II CATG 9 cut(s) 34, 70, 264, 343, 401, 407, 429, 455, 518
Kzo9I GATC 3 cut(s) 102, 351, 448
LmnI GCTCC 2 cut(s) 364, 492
LpnPI CCDG 8 cut(s) 93, 196, 234, 482, 509, 558, 606, 622
LweI GCATC 1 cut(s) 639
MaeII ACGT 2 cut(s) 139, 316
MaeIII GTNAC 1 cut(s) 185
MalI GATC 3 cut(s) 104, 353, 450
MboI GATC 3 cut(s) 102, 351, 448
MboII GAAGA 2 cut(s) 227, 320
MhlI GDGCHC 2 cut(s) 551, 633
MluCI AATT 4 cut(s) 48, 147, 440, 537
MmeI TCCRAC 1 cut(s) 400
MnlI CCTC 4 cut(s) 145, 175, 409, 472
MseI TTAA 1 cut(s) 113
MslI CAYNNNNRTG 3 cut(s) 396, 402, 573
MspI CCGG 2 cut(s) 545, 609
MspR9I CCNGG 2 cut(s) 497, 546
MvaI CCWGG 1 cut(s) 497
NciI CCSGG 1 cut(s) 546
NdeII GATC 3 cut(s) 102, 351, 448
NlaIII CATG 9 cut(s) 34, 70, 264, 343, 401, 407, 429, 455, 518
NlaIV GGNNCC 3 cut(s) 483, 494, 525
NspI RCATGY 3 cut(s) 343, 429, 518
PagI TCATGA 1 cut(s) 451
PciI ACATGT 2 cut(s) 339, 514
PfeI GAWTC 2 cut(s) 42, 670
Ppu21I YACGTR 1 cut(s) 317
PscI ACATGT 2 cut(s) 339, 514
Psp6I CCWGG 1 cut(s) 495
PspGI CCWGG 1 cut(s) 495
PspN4I GGNNCC 3 cut(s) 483, 494, 525
RsaI GTAC 3 cut(s) 55, 61, 386
RsaNI GTAC 3 cut(s) 54, 60, 385
RseI CAYNNNNRTG 3 cut(s) 396, 402, 573
SaqAI TTAA 1 cut(s) 113
Sau3AI GATC 3 cut(s) 102, 351, 448
ScrFI CCNGG 2 cut(s) 497, 546
SduI GDGCHC 2 cut(s) 551, 633
SetI ASST 8 cut(s) 142, 167, 202, 235, 319, 369, 463, 487
SfaNI GCATC 1 cut(s) 639
SmiMI CAYNNNNRTG 3 cut(s) 396, 402, 573
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 4 cut(s) 48, 147, 440, 537
SsiI CCGC 2 cut(s) 63, 606
StyD4I CCNGG 2 cut(s) 495, 544
TaiI ACGT 2 cut(s) 142, 319
TasI AATT 4 cut(s) 48, 147, 440, 537
TfiI GAWTC 2 cut(s) 42, 670
Tru1I TTAA 1 cut(s) 113
Tru9I TTAA 1 cut(s) 113
TscAI CASTG 1 cut(s) 232
TspDTI ATGAA 4 cut(s) 17, 19, 264, 423
TspRI CASTG 1 cut(s) 232
XapI RAATTY 1 cut(s) 537
XceI RCATGY 3 cut(s) 343, 429, 518
XmiI GTMKAC 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.