MD07G1079400.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000654
Physical Location & Seq
Forward (+)
7639094 .. 7640424
1331 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1079400.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGAAGGATCTTATTATGCTCCAAACTCTGCTGCTAGAAGGTTGCACAAAGCTTCAAACACTTCCAGAGCTCCCCTTAAGTTTAACAAGATTGGAAGCGAATCATTGTACATCATTGAAAAGAATAACAAATTTACCAAACATTTTCAGGTCATTGTGTAAAAGTTTTTGGTATTGTAAGAAATTAGTTGAAGCTCAAGGATTGTTCGAGATAAAACTGTTGAGCAGTGTTGACATAGATCTGATAAGAAACATGGGCTTGTTCAACTTGGAATCCACAGAAAGATCTGAGGCCGAAATGATCAACTACTTGACAAATACAACCAAGAAGTGTCCCCTCCAGGGATTAAAGGAATGCGGCATATTTAGCATTTACATTTGTGGGAGCGAGATTCCAAATTGGTTCAGCTACAGGAGCATGGGTAATTCCGTGTTGTCAGTCATTATACCTTCAGGTGTTAATCTCAAGATCCGAGGCCTAAACGCATGTGTTGTATACGCACGACAACCGGATCATAGGGGTGGTGTACAGGTTGCCAGTGAACAGTTTCTTAAAGTGAGTAATGAGACCAAGGATCGTATGTGGACCTATTCCCCCGTCACAATGGGCATTCCCAAAGAGAACGAAGACATGTTGTGGCTAAGCCGTTGGACTTTCAGAGACAATGAACTGGAGGGTGGGGAAGAAATCCGCGTTTCAGTGAAGTGTGGTTTGTGGACAAAGGAGTTTGGAATCGAACTTGTGTATGAGGACGAGAATAAGGGTAAGGATGTTGCATCAAATAGTGAAGATATACTTCCTTGGAGCCAAAGTAATGTAGTTGCTGGAGACGTGGCAGTATCAACATCAATGTATGAAATGTGGAGAGGCAAGTACTTTCTTTGTAATCATAGGTATCGAACTCATCAAGCTCAATTCAGAAGGAGGCAGGAGAACCCAGCTCATGGCGATTTTTCGTATAAACCGCCTAGTTCCTTTCATTTGAATGATTTCTTATTTAAGCACGAGGTTGATATTGCTGAAAGCAGGATAATAAAGTATCTTAAAAGAGCAGTGACAATGTTAAACCTATATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

360

Amino Acids

41.41

Weight (kDa)

7.97

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 127 - 253 1.7e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 944
AccI GTMKAC 1 cut(s) 495
AccII CGCG 1 cut(s) 693
AciI CCGC 3 cut(s) 357, 691, 965
AclWI GGATC 4 cut(s) 15, 463, 519, 582
AcsI RAATTY 1 cut(s) 130
AcuI CTGAAG 1 cut(s) 435
AfaI GTAC 3 cut(s) 109, 528, 875
AfiI CCNNNNNNNGG 2 cut(s) 341, 944
AflII CTTAAG 1 cut(s) 76
AflIII ACRYGT 1 cut(s) 630
AgsI TTSAA 5 cut(s) 56, 118, 191, 265, 985
AjiI CACGTC 1 cut(s) 832
AjnI CCWGG 1 cut(s) 339
AluBI AGCT 6 cut(s) 52, 70, 194, 408, 911, 941
AluI AGCT 6 cut(s) 52, 70, 194, 408, 911, 941
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 3 cut(s) 560, 654, 822
AlwI GGATC 4 cut(s) 15, 463, 519, 582
AoxI GGCC 2 cut(s) 291, 475
ApeKI GCWGC 1 cut(s) 31
ApoI RAATTY 1 cut(s) 130
Asp700I GAANNNNTTC 2 cut(s) 546, 989
AspS9I GGNCC 1 cut(s) 585
AvaII GGWCC 1 cut(s) 585
BanII GRGCYC 1 cut(s) 72
BauI CACGAG 1 cut(s) 1004
BbsI GAAGAC 1 cut(s) 633
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 1 cut(s) 18
BceAI ACGGC 1 cut(s) 630
BciT130I CCWGG 1 cut(s) 341
BclI TGATCA 1 cut(s) 300
BcoDI GTCTC 3 cut(s) 560, 654, 822
BfaI CTAG 2 cut(s) 35, 969
BfmI CTRYAG 1 cut(s) 409
BfrI CTTAAG 1 cut(s) 76
BglII AGATCT 2 cut(s) 238, 284
BisI GCNGC 2 cut(s) 32, 358
BlpI GCTNAGC 1 cut(s) 641
BlsI GCNGC 2 cut(s) 33, 359
BmcAI AGTACT 1 cut(s) 875
Bme1390I CCNGG 1 cut(s) 341
Bme18I GGWCC 1 cut(s) 585
BmgBI CACGTC 1 cut(s) 832
BmgT120I GGNCC 1 cut(s) 585
BmiI GGNNCC 1 cut(s) 806
BmrFI CCNGG 1 cut(s) 341
BmsI GCATC 1 cut(s) 785
BpiI GAAGAC 1 cut(s) 633
BpmI CTGGAG 3 cut(s) 323, 692, 846
Bpu1102I GCTNAGC 1 cut(s) 641
BpuEI CTTGAG 2 cut(s) 180, 449
BsaI GGTCTC 1 cut(s) 560
BsaJI CCNNGG 4 cut(s) 340, 472, 570, 800
BsaWI WCCGGW 1 cut(s) 508
BsaXI ACNNNNNCTCC 4 cut(s) 406, 436, 796, 826
Bsc4I CCNNNNNNNGG 2 cut(s) 341, 944
Bse1I ACTGG 2 cut(s) 537, 675
BseBI CCWGG 1 cut(s) 341
BseDI CCNNGG 4 cut(s) 340, 472, 570, 800
BseGI GGATG 1 cut(s) 775
BseLI CCNNNNNNNGG 2 cut(s) 341, 944
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 2 cut(s) 537, 675
BseXI GCAGC 1 cut(s) 18
BseYI CCCAGC 1 cut(s) 937
Bsh1236I CGCG 1 cut(s) 693
BshFI GGCC 2 cut(s) 293, 477
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 1 cut(s) 509
BslFI GGGAC 1 cut(s) 318
BslI CCNNNNNNNGG 2 cut(s) 341, 944
BsmAI GTCTC 3 cut(s) 560, 654, 822
BsmBI CGTCTC 1 cut(s) 822
BsmFI GGGAC 1 cut(s) 318
BsmI GAATGC 2 cut(s) 359, 609
BsnI GGCC 2 cut(s) 293, 477
Bso31I GGTCTC 1 cut(s) 560
Bsp1286I GDGCHC 1 cut(s) 72
Bsp1407I TGTACA 2 cut(s) 107, 526
Bsp143I GATC 7 cut(s) 7, 238, 284, 300, 468, 511, 574
Bsp1720I GCTNAGC 1 cut(s) 641
BspACI CCGC 3 cut(s) 357, 691, 965
BspANI GGCC 2 cut(s) 293, 477
BspCNI CTCAG 1 cut(s) 280
BspFNI CGCG 1 cut(s) 693
BspLI GGNNCC 1 cut(s) 806
BspPI GGATC 4 cut(s) 15, 463, 519, 582
BspTI CTTAAG 1 cut(s) 76
BspTNI GGTCTC 1 cut(s) 560
BsrGI TGTACA 2 cut(s) 107, 526
BsrI ACTGG 2 cut(s) 537, 675
BssECI CCNNGG 4 cut(s) 340, 472, 570, 800
BssMI GATC 7 cut(s) 7, 238, 284, 300, 468, 511, 574
BssNAI GTATAC 1 cut(s) 496
BssSI CACGAG 1 cut(s) 1004
BssT1I CCWWGG 2 cut(s) 570, 800
Bst1107I GTATAC 1 cut(s) 496
Bst2BI CACGAG 1 cut(s) 1004
Bst2UI CCWGG 1 cut(s) 341
Bst4CI ACNGT 2 cut(s) 219, 546
BstAFI CTTAAG 1 cut(s) 76
BstAUI TGTACA 2 cut(s) 107, 526
BstDEI CTNAG 2 cut(s) 288, 641
BstF5I GGATG 1 cut(s) 775
BstFNI CGCG 1 cut(s) 693
BstKTI GATC 7 cut(s) 10, 241, 287, 303, 471, 514, 577
BstMAI GTCTC 3 cut(s) 560, 654, 822
BstMBI GATC 7 cut(s) 7, 238, 284, 300, 468, 511, 574
BstMWI GCNNNNNNNGC 2 cut(s) 366, 414
BstNI CCWGG 1 cut(s) 341
BstNSI RCATGY 2 cut(s) 489, 634
BstSCI CCNGG 1 cut(s) 339
BstSFI CTRYAG 1 cut(s) 409
BstUI CGCG 1 cut(s) 693
BstV1I GCAGC 1 cut(s) 18
BstV2I GAAGAC 1 cut(s) 633
BstX2I RGATCY 4 cut(s) 7, 238, 284, 468
BstYI RGATCY 4 cut(s) 7, 238, 284, 468
BstZ17I GTATAC 1 cut(s) 496
BsuRI GGCC 2 cut(s) 293, 477
BtrI CACGTC 1 cut(s) 832
BtsCI GGATG 1 cut(s) 775
BtsI GCAGTG 2 cut(s) 232, 1059
BtsIMutI CAGTG 4 cut(s) 232, 544, 705, 1059
Cfr13I GGNCC 1 cut(s) 585
Csp6I GTAC 3 cut(s) 108, 527, 874
CviAII CATG 5 cut(s) 253, 418, 486, 631, 944
CviQI GTAC 3 cut(s) 108, 527, 874
DdeI CTNAG 2 cut(s) 288, 641
DpnI GATC 7 cut(s) 9, 240, 286, 302, 470, 513, 576
DpnII GATC 7 cut(s) 7, 238, 284, 300, 468, 511, 574
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 2 cut(s) 570, 800
Eco147I AGGCCT 1 cut(s) 477
Eco24I GRGCYC 1 cut(s) 72
Eco31I GGTCTC 1 cut(s) 560
Eco47I GGWCC 1 cut(s) 585
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 1 cut(s) 435
EcoICRI GAGCTC 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 339
EcoT14I CCWWGG 2 cut(s) 570, 800
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 2 cut(s) 570, 800
Esp3I CGTCTC 1 cut(s) 822
FaeI CATG 5 cut(s) 256, 421, 489, 634, 947
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FaqI GGGAC 1 cut(s) 318
FatI CATG 5 cut(s) 252, 417, 485, 630, 943
FbaI TGATCA 1 cut(s) 300
FblI GTMKAC 1 cut(s) 495
Fnu4HI GCNGC 2 cut(s) 32, 358
FokI GGATG 1 cut(s) 782
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 2 cut(s) 32, 358
FspBI CTAG 2 cut(s) 35, 969
GluI GCNGC 2 cut(s) 32, 358
GsaI CCCAGC 1 cut(s) 941
GsuI CTGGAG 3 cut(s) 323, 692, 846
HaeIII GGCC 2 cut(s) 293, 477
HapII CCGG 1 cut(s) 509
Hin1II CATG 5 cut(s) 256, 421, 489, 634, 947
HincII GTYRAC 1 cut(s) 232
HindII GTYRAC 1 cut(s) 232
HindIII AAGCTT 1 cut(s) 50
HinfI GANTC 4 cut(s) 100, 272, 391, 732
HpaII CCGG 1 cut(s) 509
Hpy166II GTNNAC 6 cut(s) 232, 496, 527, 542, 585, 717
Hpy188I TCNGA 5 cut(s) 243, 289, 473, 659, 920
Hpy188III TCNNGA 3 cut(s) 65, 208, 466
Hpy8I GTNNAC 6 cut(s) 232, 496, 527, 542, 585, 717
HpyAV CCTTC 3 cut(s) 32, 459, 915
HpyCH4III ACNGT 2 cut(s) 219, 546
HpyCH4IV ACGT 1 cut(s) 831
HpyCH4V TGCA 2 cut(s) 45, 776
HpyF10VI GCNNNNNNNGC 2 cut(s) 366, 414
HpyF3I CTNAG 2 cut(s) 288, 641
HpySE526I ACGT 1 cut(s) 831
Hsp92II CATG 5 cut(s) 256, 421, 489, 634, 947
Ksp22I TGATCA 1 cut(s) 300
Kzo9I GATC 7 cut(s) 7, 238, 284, 300, 468, 511, 574
LmnI GCTCC 5 cut(s) 24, 75, 384, 414, 804
Lsp1109I GCAGC 1 cut(s) 18
LweI GCATC 1 cut(s) 785
MaeI CTAG 2 cut(s) 35, 969
MaeII ACGT 1 cut(s) 831
MaeIII GTNAC 2 cut(s) 598, 1054
MalI GATC 7 cut(s) 9, 240, 286, 302, 470, 513, 576
MboI GATC 7 cut(s) 7, 238, 284, 300, 468, 511, 574
MboII GAAGA 3 cut(s) 638, 695, 800
MflI RGATCY 4 cut(s) 7, 238, 284, 468
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 5 cut(s) 130, 182, 397, 424, 914
MmeI TCCRAC 1 cut(s) 629
MnlI CCTC 8 cut(s) 283, 347, 467, 667, 742, 860, 918, 1000
MroXI GAANNNNTTC 2 cut(s) 546, 989
MseI TTAA 8 cut(s) 77, 83, 347, 459, 552, 999, 1044, 1064
MslI CAYNNNNRTG 2 cut(s) 519, 984
MspCI CTTAAG 1 cut(s) 76
MspI CCGG 1 cut(s) 509
MspR9I CCNGG 1 cut(s) 341
Mva1269I GAATGC 2 cut(s) 359, 609
MvaI CCWGG 1 cut(s) 341
MvnI CGCG 1 cut(s) 693
MwoI GCNNNNNNNGC 2 cut(s) 366, 414
NdeII GATC 7 cut(s) 7, 238, 284, 300, 468, 511, 574
NlaIII CATG 5 cut(s) 256, 421, 489, 634, 947
NlaIV GGNNCC 1 cut(s) 806
NmuCI GTSAC 2 cut(s) 598, 1054
NspI RCATGY 2 cut(s) 489, 634
PceI AGGCCT 1 cut(s) 477
PciI ACATGT 1 cut(s) 630
PctI GAATGC 2 cut(s) 359, 609
PdmI GAANNNNTTC 2 cut(s) 546, 989
PfeI GAWTC 4 cut(s) 100, 272, 391, 732
PflMI CCANNNNNTGG 1 cut(s) 944
PkrI GCNGC 2 cut(s) 33, 359
PscI ACATGT 1 cut(s) 630
Psp124BI GAGCTC 1 cut(s) 72
Psp6I CCWGG 1 cut(s) 339
PspFI CCCAGC 1 cut(s) 937
PspGI CCWGG 1 cut(s) 339
PspN4I GGNNCC 1 cut(s) 806
PspPI GGNCC 1 cut(s) 585
PsuI RGATCY 4 cut(s) 7, 238, 284, 468
RsaI GTAC 3 cut(s) 109, 528, 875
RsaNI GTAC 3 cut(s) 108, 527, 874
RseI CAYNNNNRTG 2 cut(s) 519, 984
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 8 cut(s) 77, 83, 347, 459, 552, 999, 1044, 1064
SatI GCNGC 2 cut(s) 32, 358
Sau3AI GATC 7 cut(s) 7, 238, 284, 300, 468, 511, 574
Sau96I GGNCC 1 cut(s) 585
ScaI AGTACT 1 cut(s) 875
ScrFI CCNGG 1 cut(s) 341
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 1 cut(s) 785
SfcI CTRYAG 1 cut(s) 409
SinI GGWCC 1 cut(s) 585
SmiMI CAYNNNNRTG 2 cut(s) 519, 984
SmlI CTYRAG 3 cut(s) 76, 195, 464
SmoI CTYRAG 3 cut(s) 76, 195, 464
Sse9I AATT 5 cut(s) 130, 182, 397, 424, 914
SseBI AGGCCT 1 cut(s) 477
SsiI CCGC 3 cut(s) 357, 691, 965
SspMI CTAG 2 cut(s) 35, 969
SstI GAGCTC 1 cut(s) 72
StuI AGGCCT 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 339
StyI CCWWGG 2 cut(s) 570, 800
TaaI ACNGT 2 cut(s) 219, 546
TaiI ACGT 1 cut(s) 834
TaqI TCGA 3 cut(s) 207, 735, 898
TasI AATT 5 cut(s) 130, 182, 397, 424, 914
TatI WGTACW 3 cut(s) 107, 526, 873
TauI GCSGC 1 cut(s) 360
TfiI GAWTC 4 cut(s) 100, 272, 391, 732
Tru1I TTAA 8 cut(s) 77, 83, 347, 459, 552, 999, 1044, 1064
Tru9I TTAA 8 cut(s) 77, 83, 347, 459, 552, 999, 1044, 1064
TscAI CASTG 4 cut(s) 232, 544, 705, 1059
TseFI GTSAC 2 cut(s) 598, 1054
TseI GCWGC 1 cut(s) 31
Tsp45I GTSAC 2 cut(s) 598, 1054
TspDTI ATGAA 4 cut(s) 17, 681, 870, 968
TspGWI ACGGA 1 cut(s) 418
TspRI CASTG 4 cut(s) 232, 544, 705, 1059
Van91I CCANNNNNTGG 1 cut(s) 944
Vha464I CTTAAG 1 cut(s) 76
VpaK11BI GGWCC 1 cut(s) 585
XapI RAATTY 1 cut(s) 130
XceI RCATGY 2 cut(s) 489, 634
XmiI GTMKAC 1 cut(s) 495
XmnI GAANNNNTTC 2 cut(s) 546, 989
XspI CTAG 2 cut(s) 35, 969
ZrmI AGTACT 1 cut(s) 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.