Rh6BG300700

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000654
Physical Location & Seq
Forward (+)
53005223 .. 53006322
1100 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG300700.1

Sequence Viewer

Length: 963 bp
ATGCTTCCGGAGCTCCCACCCAATTTAGAAATGTTTTTTGCAATCAATTGTACATCAGTGAAAAAACTTCCAAGTTTACCTAATACATCGATGAATATACTTATCGGTTGTGGAGAATTAGTTGAAGTTCAAAGTGTCTTCAGTATAAGGCCATTGATCGGCGAGCTTGACATGGAAATGATAAGAAATATGGGCCTGTGCAATTTGGAACCCTTTGGACGCACTGAGGAGCTTGAAATGATAAATTGCTTGACATCAACAACCAGGACATGTCCCCTCCAGGGGCTGTATGAATGTGGCATATTCAGCATTTTCCTTCATGGAAGCATGATTCCTAATTGGTACAAGTACAGGGGTGAAACCGCCTTGTCTATCATTGTTCCTTCCCATCCTAAACTCAAGATTAAAGGCTTAAATGTCTGTGTTGTGTACACCAGAGATTGTGTTGTGCACAAATTTGCGTTGTATATTTCACTCTTTCTAAAAGTCAGTAATGAAGCGAAGGGTCTTATGTGGTCATATTCCCCTGTTAGTGTTGGTCGCCCAAAAGAGAATGAAGAAATGTCATGGCTAAGCCATTGGCAGTTTGAGAATGATGAGTTAGAGGGTGGGGAAGAACTATGTTTTTCTGTCGATACCGAAGATGTTCGGTTCTTGATAAAGGAGATTGGAGTCCAACTTGTGTACGAGCATGAAAATGAGGGCAAGGCATGTATCCAATCATCAATTCCTGAAGATGTTACAACCCAACATGAGGCACCTTCTTGGAGCCAGGGTGTCCTCATTGAACATGTAGCGGCACCAAAGTATCAAATTTGGCCGGGCAAATACTTCCTTTGCAATCATTACTCATTTCCTGGTCGTTTTGATTTTTCAAAAGCATACCCCATGGGTGGTTACCGCCGTATATTGGAACACCAAGATTTTGAGAAACGTGTCTTGGGACAAAAAAATCTTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

320

Amino Acids

36.58

Weight (kDa)

5.46

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 757, 799
AccIII TCCGGA 1 cut(s) 7
AciI CCGC 3 cut(s) 363, 797, 901
AcoI YGGCCR 1 cut(s) 818
AcsI RAATTY 2 cut(s) 455, 813
AcuI CTGAAG 2 cut(s) 124, 753
AfaI GTAC 5 cut(s) 52, 344, 350, 431, 686
AfiI CCNNNNNNNGG 6 cut(s) 158, 281, 282, 754, 893, 910
AflIII ACRYGT 3 cut(s) 269, 790, 934
AgsI TTSAA 5 cut(s) 125, 131, 236, 788, 876
AjnI CCWGG 4 cut(s) 263, 279, 771, 856
AjuI GAANNNNNNNTTGG 4 cut(s) 711, 743, 923, 955
AluBI AGCT 3 cut(s) 13, 166, 232
AluI AGCT 3 cut(s) 13, 166, 232
Alw21I GWGCWC 2 cut(s) 15, 453
Alw44I GTGCAC 1 cut(s) 449
AlwNI CAGNNNCTG 1 cut(s) 286
Aor13HI TCCGGA 1 cut(s) 7
AoxI GGCC 3 cut(s) 149, 193, 818
ApaLI GTGCAC 1 cut(s) 449
ApoI RAATTY 2 cut(s) 455, 813
Asp700I GAANNNNTTC 1 cut(s) 645
AspS9I GGNCC 1 cut(s) 193
AsuC2I CCSGG 1 cut(s) 822
AsuHPI GGTGA 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 453
BaeI ACNNNNGTAYC 2 cut(s) 791, 824
BanI GGYRCC 2 cut(s) 757, 799
BanII GRGCYC 1 cut(s) 15
BbsI GAAGAC 1 cut(s) 130
Bbv12I GWGCWC 2 cut(s) 15, 453
BccI CCATC 1 cut(s) 396
BceAI ACGGC 1 cut(s) 888
BciT130I CCWGG 4 cut(s) 265, 281, 773, 858
BciVI GTATCC 1 cut(s) 725
BcnI CCSGG 1 cut(s) 822
BfuI GTATCC 1 cut(s) 725
BisI GCNGC 1 cut(s) 798
BlpI GCTNAGC 1 cut(s) 572
BlsI GCNGC 1 cut(s) 799
Bme1390I CCNGG 5 cut(s) 265, 281, 773, 822, 858
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 4 cut(s) 210, 759, 770, 801
BmrFI CCNGG 5 cut(s) 265, 281, 773, 822, 858
BpiI GAAGAC 1 cut(s) 130
BpmI CTGGAG 1 cut(s) 263
Bpu1102I GCTNAGC 1 cut(s) 572
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 1 cut(s) 822
Bsa29I ATCGAT 1 cut(s) 89
BsaJI CCNNGG 3 cut(s) 280, 772, 888
BsaWI WCCGGW 1 cut(s) 7
BsaXI ACNNNNNCTCC 4 cut(s) 105, 135, 760, 790
Bsc4I CCNNNNNNNGG 6 cut(s) 158, 281, 282, 754, 893, 910
BseAI TCCGGA 1 cut(s) 7
BseBI CCWGG 4 cut(s) 265, 281, 773, 858
BseCI ATCGAT 1 cut(s) 89
BseDI CCNNGG 3 cut(s) 280, 772, 888
BseGI GGATG 1 cut(s) 388
BseLI CCNNNNNNNGG 6 cut(s) 158, 281, 282, 754, 893, 910
BseMII CTCAG 1 cut(s) 216
BseRI GAGGAG 1 cut(s) 242
BseSI GKGCMC 1 cut(s) 453
BshFI GGCC 3 cut(s) 151, 195, 820
BshNI GGYRCC 2 cut(s) 757, 799
BshVI ATCGAT 1 cut(s) 89
BsiHKAI GWGCWC 2 cut(s) 15, 453
BsiSI CCGG 2 cut(s) 8, 821
BslFI GGGAC 2 cut(s) 258, 957
BslI CCNNNNNNNGG 6 cut(s) 158, 281, 282, 754, 893, 910
BsmFI GGGAC 2 cut(s) 258, 957
BsnI GGCC 3 cut(s) 151, 195, 820
Bsp1286I GDGCHC 2 cut(s) 15, 453
Bsp13I TCCGGA 1 cut(s) 7
Bsp1407I TGTACA 2 cut(s) 50, 429
Bsp143I GATC 1 cut(s) 156
Bsp1720I GCTNAGC 1 cut(s) 572
Bsp19I CCATGG 1 cut(s) 888
BspACI CCGC 3 cut(s) 363, 797, 901
BspANI GGCC 3 cut(s) 151, 195, 820
BspCNI CTCAG 1 cut(s) 217
BspDI ATCGAT 1 cut(s) 89
BspEI TCCGGA 1 cut(s) 7
BspLI GGNNCC 4 cut(s) 210, 759, 770, 801
BspT107I GGYRCC 2 cut(s) 757, 799
BsrGI TGTACA 2 cut(s) 50, 429
BssECI CCNNGG 3 cut(s) 280, 772, 888
BssMI GATC 1 cut(s) 156
BssT1I CCWWGG 1 cut(s) 888
Bst2UI CCWGG 4 cut(s) 265, 281, 773, 858
BstAUI TGTACA 2 cut(s) 50, 429
BstC8I GCNNGC 1 cut(s) 164
BstDEI CTNAG 2 cut(s) 225, 572
BstDSI CCRYGG 1 cut(s) 888
BstEII GGTNACC 1 cut(s) 896
BstF5I GGATG 1 cut(s) 388
BstKTI GATC 1 cut(s) 159
BstMBI GATC 1 cut(s) 156
BstMWI GCNNNNNNNGC 2 cut(s) 10, 306
BstNI CCWGG 4 cut(s) 265, 281, 773, 858
BstNSI RCATGY 3 cut(s) 273, 714, 794
BstPI GGTNACC 1 cut(s) 896
BstSCI CCNGG 5 cut(s) 263, 279, 771, 820, 856
BstSLI GKGCMC 1 cut(s) 453
BstV2I GAAGAC 1 cut(s) 130
Bsu15I ATCGAT 1 cut(s) 89
BsuI GTATCC 1 cut(s) 725
BsuRI GGCC 3 cut(s) 151, 195, 820
BsuTUI ATCGAT 1 cut(s) 89
BtgI CCRYGG 1 cut(s) 888
BtsCI GGATG 1 cut(s) 388
BtsIMutI CAGTG 2 cut(s) 63, 222
Cac8I GCNNGC 1 cut(s) 164
CaiI CAGNNNCTG 1 cut(s) 286
Cfr13I GGNCC 1 cut(s) 193
ClaI ATCGAT 1 cut(s) 89
CseI GACGC 1 cut(s) 228
Csp6I GTAC 5 cut(s) 51, 343, 349, 430, 685
CviQI GTAC 5 cut(s) 51, 343, 349, 430, 685
DdeI CTNAG 2 cut(s) 225, 572
DpnI GATC 1 cut(s) 158
DpnII GATC 1 cut(s) 156
EaeI YGGCCR 1 cut(s) 818
Ecl136II GAGCTC 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 888
Eco24I GRGCYC 1 cut(s) 15
Eco53kI GAGCTC 1 cut(s) 13
Eco57I CTGAAG 2 cut(s) 124, 753
Eco91I GGTNACC 1 cut(s) 896
EcoICRI GAGCTC 1 cut(s) 13
EcoO65I GGTNACC 1 cut(s) 896
EcoRII CCWGG 4 cut(s) 263, 279, 771, 856
EcoT14I CCWWGG 1 cut(s) 888
EcoT38I GRGCYC 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 888
FaqI GGGAC 2 cut(s) 258, 957
Fnu4HI GCNGC 1 cut(s) 798
FokI GGATG 1 cut(s) 375
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 1 cut(s) 798
GluI GCNGC 1 cut(s) 798
GsuI CTGGAG 1 cut(s) 263
HaeIII GGCC 3 cut(s) 151, 195, 820
HapII CCGG 2 cut(s) 8, 821
HgaI GACGC 1 cut(s) 228
HinfI GANTC 2 cut(s) 331, 672
HpaII CCGG 2 cut(s) 8, 821
HphI GGTGA 1 cut(s) 368
Hpy166II GTNNAC 5 cut(s) 77, 430, 432, 451, 685
Hpy188III TCNNGA 4 cut(s) 8, 400, 655, 731
Hpy8I GTNNAC 5 cut(s) 77, 430, 432, 451, 685
HpyAV CCTTC 4 cut(s) 326, 393, 496, 771
HpyCH4IV ACGT 1 cut(s) 934
HpyCH4V TGCA 4 cut(s) 41, 201, 451, 840
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 306
HpyF3I CTNAG 2 cut(s) 225, 572
HpySE526I ACGT 1 cut(s) 934
Kpn2I TCCGGA 1 cut(s) 7
Kzo9I GATC 1 cut(s) 156
LmnI GCTCC 4 cut(s) 10, 18, 229, 768
MaeII ACGT 1 cut(s) 934
MaeIII GTNAC 2 cut(s) 739, 896
MalI GATC 1 cut(s) 158
MboI GATC 1 cut(s) 156
MboII GAAGA 5 cut(s) 130, 569, 626, 653, 746
MfeI CAATTG 1 cut(s) 46
MhlI GDGCHC 2 cut(s) 15, 453
MluCI AATT 9 cut(s) 22, 46, 116, 202, 244, 337, 455, 726, 813
MlyI GAGTC 1 cut(s) 681
MmeI TCCRAC 1 cut(s) 700
MnlI CCTC 6 cut(s) 220, 287, 598, 694, 748, 791
MroI TCCGGA 1 cut(s) 7
MroXI GAANNNNTTC 1 cut(s) 645
MseI TTAA 2 cut(s) 405, 413
MslI CAYNNNNRTG 2 cut(s) 176, 696
MspI CCGG 2 cut(s) 8, 821
MspR9I CCNGG 5 cut(s) 265, 281, 773, 822, 858
MunI CAATTG 1 cut(s) 46
MvaI CCWGG 4 cut(s) 265, 281, 773, 858
MwoI GCNNNNNNNGC 2 cut(s) 10, 306
NciI CCSGG 1 cut(s) 822
NcoI CCATGG 1 cut(s) 888
NdeII GATC 1 cut(s) 156
NlaIV GGNNCC 4 cut(s) 210, 759, 770, 801
NspI RCATGY 3 cut(s) 273, 714, 794
PciI ACATGT 2 cut(s) 269, 790
PdmI GAANNNNTTC 1 cut(s) 645
PfeI GAWTC 1 cut(s) 331
PkrI GCNGC 1 cut(s) 799
PleI GAGTC 1 cut(s) 680
PpsI GAGTC 1 cut(s) 680
PscI ACATGT 2 cut(s) 269, 790
Psp124BI GAGCTC 1 cut(s) 15
Psp6I CCWGG 4 cut(s) 263, 279, 771, 856
PspEI GGTNACC 1 cut(s) 896
PspGI CCWGG 4 cut(s) 263, 279, 771, 856
PspN4I GGNNCC 4 cut(s) 210, 759, 770, 801
PspPI GGNCC 1 cut(s) 193
PstNI CAGNNNCTG 1 cut(s) 286
RsaI GTAC 5 cut(s) 52, 344, 350, 431, 686
RsaNI GTAC 5 cut(s) 51, 343, 349, 430, 685
RseI CAYNNNNRTG 2 cut(s) 176, 696
SacI GAGCTC 1 cut(s) 15
SaqAI TTAA 2 cut(s) 405, 413
SatI GCNGC 1 cut(s) 798
Sau3AI GATC 1 cut(s) 156
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 1 cut(s) 681
ScrFI CCNGG 5 cut(s) 265, 281, 773, 822, 858
SduI GDGCHC 2 cut(s) 15, 453
SetI ASST 6 cut(s) 15, 82, 168, 234, 763, 937
SmiMI CAYNNNNRTG 2 cut(s) 176, 696
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 9 cut(s) 22, 46, 116, 202, 244, 337, 455, 726, 813
SsiI CCGC 3 cut(s) 363, 797, 901
SstI GAGCTC 1 cut(s) 15
StyD4I CCNGG 5 cut(s) 263, 279, 771, 820, 856
StyI CCWWGG 1 cut(s) 888
TaiI ACGT 1 cut(s) 937
TaqI TCGA 2 cut(s) 89, 633
TasI AATT 9 cut(s) 22, 46, 116, 202, 244, 337, 455, 726, 813
TatI WGTACW 3 cut(s) 50, 348, 429
TauI GCSGC 1 cut(s) 800
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 2 cut(s) 405, 413
Tru9I TTAA 2 cut(s) 405, 413
TscAI CASTG 2 cut(s) 63, 229
TspDTI ATGAA 6 cut(s) 107, 306, 308, 510, 570, 708
TspRI CASTG 2 cut(s) 63, 229
VneI GTGCAC 1 cut(s) 449
XapI RAATTY 2 cut(s) 455, 813
XceI RCATGY 3 cut(s) 273, 714, 794
XmnI GAANNNNTTC 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.