MD17G1274300.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000654
Physical Location & Seq
Forward (+)
33584520 .. 33587272
2753 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1274300.v1.1.491

Sequence Viewer

Length: 2301 bp
ATGGATTATACAAGTGCAATGTTGGACAGCTTAGAGTTTTGCACAAGGATTGGGATTGAAAATCTAGTTGACAGATACCTAGTGGAAATTCAAGGGTCGAAGTTGGTCATGCATCAATTGGTGAGAAACATGGCAATGGCAATTATTCGCGAAGAATCACCTGATGATCCTGGAAAACGTAGTAGAGTGTTACAGAATGATGCTTCTAATATTTTGAGAAAATTAAGTGGCACGGAAAATATTAAGGGCCTCATGCTCAACCTTGCTAGCAAAAGAACGTTTGTTGGAAGTGATAAAAAACAATGCCATGTTGAAGATTATGATAGAAATTGTTCAAGACAACGTTGGCTTGGTTTCTTGTCTTGGAAGCCAATTAGCTTTTCCTCCACAAACTCAGCTTCTGCATCAAATGAGATAGATTTCAAATCTGAGGCGTTTAGAAGGATGCACAATCTTGAAATTCTCATTCTCAACAACGTCAACGTCGAGGGAAGCTACGAAGAATTTTCAAAAAGTTTAATATGTTTATCTTGGAGAGGATTCCACTTAAAATCAATACCAGAAAATTTTTATTTGGAAAACCTGGTTGTTCTTGACATGCGAAATAGTAGCCTACAACATGTTTGGAATGGGATCAGGTTTCTTCCGAGGCTGAAAATTCTTAATCTCAGTCATTCGCATGGCCTTAGGACAACCTCCGACTTCTCAGGACTCCCTAACCTTGAGAGATTGATTCTTAAAGATTGCATAAATTTGGTTGAGGTTGATGAGTCCATCGGATACCTAGAGAAACTTGTTGTTTTGAATCTAGAAGGCTGCAAAAATCTCATGAAGCTTCCAAAATTGAGATCTATTCAGGATCTTATTCTTTCTGGTTGCTCAAAGCTTGTGCTTGGTGCCAATACGACTGCCACAACTGGTCACTTACAATCTACAGCTTGTGAAATGAAGAAACTCAATCTGCTATCAGCTAAATCATGGTATTCAATCTGGTCGCAGGTGTCGCCAAGAAAAAATATTGAACCAAGCAGTTTCTCATTGGCAAGTTTACCACATTCCTTGACAAGTTTACGTCTGGATAGCTGCAATCTATCAGAGATTCCGAGTGCCCTTACTATGCTGTCTTCGTTAGAGTACTTGGATCTGTATAACAATCCAATTACAAGCCTACCAGAGAGCATGAACAATCTTGTTAGGCTTCACACTCTTGCAGTAGAAGGTTGCAGAAACCTCACAATGCTTCCAGAGCTCCCACATAGTTTGAAACAATTGAATGGTAGAGGTTGCACATCATTGAAAAGAATAACAAATTTACCGAACTTGCACATATCACCTACCGTATATCCCTTCGGCATGCAAAAGGACTTAGATTTAATGCATTACAATATAGTATTGGACTCAAATTTATGGTATTGTGGGAAACTAGTTGATGTTGCAAGCTTGTTCAATACACTACCATTGAGCAGCTTTGACATAAAACTCCTCAAAGATATGGATCTATTCGATTTGAAACCCAACGGTGGCGTAGAGGTCCAAAGGAACAACGATTGTGCAAGTTTTAGCAAGCCAAGGCGCTCCAAGGGAACTTTTGAATGCGGCATAATTAGCATTTTTGTGCACGAGAGCAAGATTCCAGATCGGTTCAATTACAGGTGGATGCCTCCAGATCGGTTCGATTACAGGAGCATGGGTAACACTGTGTTCTCTATTATTGTTCCTTCACAGCCTAATATCAAGATCGTAGGTTTAAATGCATGTATACTGTATGCCCGGCAATCGGATCGAAGCCCTGTGATTCCATTTGGGATTCCATTTGGAAACTCTCATGCCCATTCGCTTGAACTTAGGAATGAGACAAAGGGCCTTAAGTGGAACTTAAGAATGTCGACTGGGGTTGAATGGAAAGTGATGAACGAAGATATGTTATGGCTAAGCCATTGGATTATGGGGAACAATGAATTGGAGTGCGGGGAAAAAGTGTATTTTAGACTAACCGAAGATCATGACGATTTATTCACAAAGGGGATTGGAGTCCAGTTTGGGTACGAGCAGCAAAATAAGGATAAGGAGGATGTCCCATCAAGTAGTGAAGATCCAATGATCCTACCTGACAGATCGCCTTGGGACGGCGGTCACGCTTCCAGCTGGGTAACGGATTCATGTCAGAGATTTATCGGGCCGAAATGGATCGAGAACCTTAAGAAGGAATCTATTTCAACTGTCGTGTGCGCGGCTAATTACTCTCTCTGTAACCATGAAGTGATCGACCTTGAGAACGAGTCCCGCATGACGCCATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

767

Amino Acids

87.1

Weight (kDa)

6.71

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 7 - 53 1.6e-06 Disease resistance protein Roq1-like, winged-helix domain
LRR_8 PF13855 353 - 408 5.3e-07 Leucine rich repeat
LRR_4 PF12799 353 - 391 6.9e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 354 - 444 5.5e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 988
Acc36I ACCTGC 1 cut(s) 988
AccB1I GGYRCC 1 cut(s) 896
AccI GTMKAC 2 cut(s) 1759, 1886
AccII CGCG 2 cut(s) 150, 2231
AciI CCGC 5 cut(s) 1596, 1968, 2130, 2231, 2284
AclI AACGTT 2 cut(s) 278, 343
AclWI GGATC 9 cut(s) 161, 641, 867, 1149, 1503, 1788, 2087, 2095, 2195
AcsI RAATTY 8 cut(s) 87, 459, 503, 565, 657, 751, 1309, 1402
AcyI GRCGYC 1 cut(s) 2291
AfaI GTAC 2 cut(s) 1136, 2045
AfiI CCNNNNNNNGG 4 cut(s) 1520, 1777, 2126, 2203
AflII CTTAAG 3 cut(s) 1865, 1876, 2198
AflIII ACRYGT 1 cut(s) 619
AhlI ACTAGT 1 cut(s) 1423
AjnI CCWGG 2 cut(s) 169, 582
AjuI GAANNNNNNNTTGG 4 cut(s) 364, 396, 1943, 1975
Alw21I GWGCWC 2 cut(s) 1251, 1620
Alw26I GTCTC 1 cut(s) 1847
Alw44I GTGCAC 1 cut(s) 1616
AlwI GGATC 9 cut(s) 161, 641, 867, 1149, 1503, 1788, 2087, 2095, 2195
AlwNI CAGNNNCTG 1 cut(s) 401
AoxI GGCC 4 cut(s) 247, 682, 1861, 2177
ApaLI GTGCAC 1 cut(s) 1616
ApeKI GCWGC 4 cut(s) 816, 1083, 1464, 2050
ApoI RAATTY 8 cut(s) 87, 459, 503, 565, 657, 751, 1309, 1402
Asp700I GAANNNNTTC 1 cut(s) 331
AspLEI GCGC 2 cut(s) 1575, 2231
AspS9I GGNCC 4 cut(s) 247, 1531, 1861, 2177
AsuC2I CCSGG 1 cut(s) 1771
AsuHPI GGTGA 3 cut(s) 133, 150, 1323
AsuNHI GCTAGC 1 cut(s) 266
AvaII GGWCC 1 cut(s) 1531
AxyI CCTNAGG 1 cut(s) 686
BaeGI GKGCMC 2 cut(s) 1111, 1620
BanI GGYRCC 1 cut(s) 896
BanII GRGCYC 1 cut(s) 1251
BauI CACGAG 1 cut(s) 1619
BbsI GAAGAC 1 cut(s) 1116
Bbv12I GWGCWC 2 cut(s) 1251, 1620
BbvI GCAGC 4 cut(s) 803, 1070, 1476, 2062
BccI CCATC 2 cut(s) 782, 2086
BceAI ACGGC 1 cut(s) 2143
BcgI CGANNNNNNTGC 2 cut(s) 1763, 1797
BciT130I CCWGG 2 cut(s) 171, 584
BciVI GTATCC 1 cut(s) 773
BcnI CCSGG 1 cut(s) 1771
BcoDI GTCTC 1 cut(s) 1847
BcuI ACTAGT 1 cut(s) 1423
BfaI CTAG 6 cut(s) 65, 80, 267, 785, 809, 1424
BfmI CTRYAG 1 cut(s) 933
BfoI RGCGCY 1 cut(s) 1576
BfrI CTTAAG 3 cut(s) 1865, 1876, 2198
BfuAI ACCTGC 1 cut(s) 988
BfuI GTATCC 1 cut(s) 773
BglII AGATCT 1 cut(s) 848
BisI GCNGC 6 cut(s) 817, 1084, 1465, 1597, 2051, 2232
BlpI GCTNAGC 1 cut(s) 1931
BlsI GCNGC 6 cut(s) 818, 1085, 1466, 1598, 2052, 2233
BmcAI AGTACT 1 cut(s) 1136
Bme1390I CCNGG 3 cut(s) 171, 584, 1771
Bme18I GGWCC 1 cut(s) 1531
BmgT120I GGNCC 4 cut(s) 247, 1531, 1861, 2177
BmiI GGNNCC 1 cut(s) 898
BmrFI CCNGG 3 cut(s) 171, 584, 1771
BmrI ACTGGG 1 cut(s) 1899
BmsI GCATC 5 cut(s) 121, 190, 413, 435, 1647
BmtI GCTAGC 1 cut(s) 270
BmuI ACTGGG 1 cut(s) 1899
BoxI GACNNNNGTC 1 cut(s) 2130
BpiI GAAGAC 1 cut(s) 1116
BpmI CTGGAG 1 cut(s) 1647
Bpu1102I GCTNAGC 1 cut(s) 1931
BpuEI CTTGAG 2 cut(s) 743, 2291
BpuMI CCSGG 1 cut(s) 1771
BsaBI GATNNNNATC 1 cut(s) 1494
BsaHI GRCGYC 1 cut(s) 2291
BsaJI CCNNGG 5 cut(s) 647, 1568, 1578, 2120, 2294
BsaXI ACNNNNNCTCC 2 cut(s) 1675, 1705
Bsc4I CCNNNNNNNGG 4 cut(s) 1520, 1777, 2126, 2203
Bse1I ACTGG 3 cut(s) 922, 1894, 2035
Bse21I CCTNAGG 1 cut(s) 686
Bse3DI GCAATG 2 cut(s) 24, 141
Bse8I GATNNNNATC 1 cut(s) 1494
BseBI CCWGG 2 cut(s) 171, 584
BseDI CCNNGG 5 cut(s) 647, 1568, 1578, 2120, 2294
BseGI GGATG 3 cut(s) 450, 1662, 2077
BseJI GATNNNNATC 1 cut(s) 1494
BseLI CCNNNNNNNGG 4 cut(s) 1520, 1777, 2126, 2203
BseMI GCAATG 2 cut(s) 24, 141
BseMII CTCAG 4 cut(s) 408, 420, 682, 720
BseNI ACTGG 3 cut(s) 922, 1894, 2035
BseRI GAGGAG 1 cut(s) 1472
BseSI GKGCMC 2 cut(s) 1111, 1620
BseXI GCAGC 4 cut(s) 803, 1070, 1476, 2062
BseYI CCCAGC 1 cut(s) 2145
Bsh1236I CGCG 2 cut(s) 150, 2231
BshFI GGCC 4 cut(s) 249, 684, 1863, 2179
BshNI GGYRCC 1 cut(s) 896
BsiHKAI GWGCWC 2 cut(s) 1251, 1620
BsiSI CCGG 1 cut(s) 1771
BslFI GGGAC 3 cut(s) 2060, 2138, 2266
BslI CCNNNNNNNGG 4 cut(s) 1520, 1777, 2126, 2203
BsmAI GTCTC 1 cut(s) 1847
BsmFI GGGAC 3 cut(s) 2060, 2138, 2266
BsmI GAATGC 1 cut(s) 1598
BsnI GGCC 4 cut(s) 249, 684, 1863, 2179
Bsp1286I GDGCHC 3 cut(s) 1111, 1251, 1620
Bsp1720I GCTNAGC 1 cut(s) 1931
Bsp19I CCATGG 1 cut(s) 2294
Bsp68I TCGCGA 1 cut(s) 150
BspACI CCGC 5 cut(s) 1596, 1968, 2130, 2231, 2284
BspANI GGCC 4 cut(s) 249, 684, 1863, 2179
BspCNI CTCAG 4 cut(s) 407, 421, 681, 719
BspFNI CGCG 2 cut(s) 150, 2231
BspHI TCATGA 2 cut(s) 828, 2002
BspLI GGNNCC 1 cut(s) 898
BspMI ACCTGC 1 cut(s) 988
BspOI GCTAGC 1 cut(s) 270
BspPI GGATC 9 cut(s) 161, 641, 867, 1149, 1503, 1788, 2087, 2095, 2195
BspT107I GGYRCC 1 cut(s) 896
BspTI CTTAAG 3 cut(s) 1865, 1876, 2198
BsrDI GCAATG 2 cut(s) 24, 141
BsrI ACTGG 3 cut(s) 922, 1894, 2035
BssECI CCNNGG 5 cut(s) 647, 1568, 1578, 2120, 2294
BssNAI GTATAC 1 cut(s) 1760
BssNI GRCGYC 1 cut(s) 2291
BssSI CACGAG 1 cut(s) 1619
BssT1I CCWWGG 4 cut(s) 1568, 1578, 2120, 2294
Bst1107I GTATAC 1 cut(s) 1760
Bst2BI CACGAG 1 cut(s) 1619
Bst2UI CCWGG 2 cut(s) 171, 584
Bst4CI ACNGT 5 cut(s) 1339, 1520, 1699, 1764, 2221
BstACI GRCGYC 1 cut(s) 2291
BstAFI CTTAAG 3 cut(s) 1865, 1876, 2198
BstC8I GCNNGC 4 cut(s) 268, 1355, 1438, 1565
BstDEI CTNAG 9 cut(s) 31, 394, 429, 668, 686, 706, 1366, 1844, 1931
BstDSI CCRYGG 1 cut(s) 2294
BstENI CCTNNNNNAGG 1 cut(s) 2201
BstF5I GGATG 3 cut(s) 450, 1662, 2077
BstFNI CGCG 2 cut(s) 150, 2231
BstH2I RGCGCY 1 cut(s) 1576
BstHHI GCGC 2 cut(s) 1575, 2231
BstMAI GTCTC 1 cut(s) 1847
BstMWI GCNNNNNNNGC 3 cut(s) 1003, 1246, 1605
BstNI CCWGG 2 cut(s) 171, 584
BstNSI RCATGY 4 cut(s) 601, 623, 1357, 1758
BstPAI GACNNNNGTC 1 cut(s) 2130
BstSCI CCNGG 3 cut(s) 169, 582, 1769
BstSFI CTRYAG 1 cut(s) 933
BstSLI GKGCMC 2 cut(s) 1111, 1620
BstUI CGCG 2 cut(s) 150, 2231
BstV1I GCAGC 4 cut(s) 803, 1070, 1476, 2062
BstV2I GAAGAC 1 cut(s) 1116
BstX2I RGATCY 5 cut(s) 848, 859, 1141, 1495, 2092
BstYI RGATCY 5 cut(s) 848, 859, 1141, 1495, 2092
BstZ17I GTATAC 1 cut(s) 1760
Bsu36I CCTNAGG 1 cut(s) 686
BsuI GTATCC 1 cut(s) 773
BsuRI GGCC 4 cut(s) 249, 684, 1863, 2179
BtgI CCRYGG 1 cut(s) 2294
BtsCI GGATG 3 cut(s) 450, 1662, 2077
BtsIMutI CAGTG 1 cut(s) 1695
BtuMI TCGCGA 1 cut(s) 150
BveI ACCTGC 1 cut(s) 988
Cac8I GCNNGC 4 cut(s) 268, 1355, 1438, 1565
CaiI CAGNNNCTG 1 cut(s) 401
CciI TCATGA 2 cut(s) 828, 2002
CfoI GCGC 2 cut(s) 1575, 2231
Cfr13I GGNCC 4 cut(s) 247, 1531, 1861, 2177
CsiI ACCWGGT 1 cut(s) 582
Csp6I GTAC 2 cut(s) 1135, 2044
CspCI CAANNNNNGTGG 4 cut(s) 1041, 1076, 1242, 1277
CviQI GTAC 2 cut(s) 1135, 2044
DdeI CTNAG 9 cut(s) 31, 394, 429, 668, 686, 706, 1366, 1844, 1931
DraI TTTAAA 1 cut(s) 1749
Ecl136II GAGCTC 1 cut(s) 1249
Eco130I CCWWGG 4 cut(s) 1568, 1578, 2120, 2294
Eco24I GRGCYC 1 cut(s) 1251
Eco47I GGWCC 1 cut(s) 1531
Eco53kI GAGCTC 1 cut(s) 1249
Eco81I CCTNAGG 1 cut(s) 686
EcoICRI GAGCTC 1 cut(s) 1249
EcoNI CCTNNNNNAGG 1 cut(s) 2201
EcoO109I RGGNCCY 2 cut(s) 247, 1861
EcoRII CCWGG 2 cut(s) 169, 582
EcoT14I CCWWGG 4 cut(s) 1568, 1578, 2120, 2294
EcoT22I ATGCAT 3 cut(s) 114, 1380, 1756
EcoT38I GRGCYC 1 cut(s) 1251
ErhI CCWWGG 4 cut(s) 1568, 1578, 2120, 2294
FalI AAGNNNNNCTT 2 cut(s) 1859, 1891
FaqI GGGAC 3 cut(s) 2060, 2138, 2266
FauI CCCGC 2 cut(s) 1961, 2291
FblI GTMKAC 2 cut(s) 1759, 1886
Fnu4HI GCNGC 6 cut(s) 817, 1084, 1465, 1597, 2051, 2232
FokI GGATG 3 cut(s) 457, 1669, 2084
FriOI GRGCYC 1 cut(s) 1251
Fsp4HI GCNGC 6 cut(s) 817, 1084, 1465, 1597, 2051, 2232
FspBI CTAG 6 cut(s) 65, 80, 267, 785, 809, 1424
GlaI GCGC 2 cut(s) 1574, 2230
GluI GCNGC 6 cut(s) 817, 1084, 1465, 1597, 2051, 2232
GsaI CCCAGC 1 cut(s) 2149
GsuI CTGGAG 1 cut(s) 1647
HaeII RGCGCY 1 cut(s) 1576
HaeIII GGCC 4 cut(s) 249, 684, 1863, 2179
HapII CCGG 1 cut(s) 1771
HhaI GCGC 2 cut(s) 1575, 2231
Hin1I GRCGYC 1 cut(s) 2291
Hin6I GCGC 2 cut(s) 1573, 2229
HinP1I GCGC 2 cut(s) 1573, 2229
HincII GTYRAC 3 cut(s) 70, 481, 1887
HindII GTYRAC 3 cut(s) 70, 481, 1887
HindIII AAGCTT 3 cut(s) 833, 884, 1438
HpaII CCGG 1 cut(s) 1771
HphI GGTGA 3 cut(s) 133, 150, 1323
Hpy166II GTNNAC 7 cut(s) 70, 481, 1049, 1070, 1618, 1760, 1887
Hpy188I TCNGA 8 cut(s) 430, 648, 700, 779, 1096, 1104, 1780, 2166
Hpy8I GTNNAC 7 cut(s) 70, 481, 1049, 1070, 1618, 1760, 1887
Hpy99I CGWCG 1 cut(s) 488
HpyAV CCTTC 6 cut(s) 435, 806, 1211, 1357, 1728, 2197
HpyCH4III ACNGT 5 cut(s) 1339, 1520, 1699, 1764, 2221
HpyCH4IV ACGT 6 cut(s) 178, 278, 343, 477, 483, 1072
HpyF10VI GCNNNNNNNGC 3 cut(s) 1003, 1246, 1605
HpyF3I CTNAG 9 cut(s) 31, 394, 429, 668, 686, 706, 1366, 1844, 1931
HpySE526I ACGT 6 cut(s) 178, 278, 343, 477, 483, 1072
Hsp92I GRCGYC 1 cut(s) 2291
HspAI GCGC 2 cut(s) 1573, 2229
LmnI GCTCC 3 cut(s) 1254, 1580, 1683
Lsp1109I GCAGC 4 cut(s) 803, 1070, 1476, 2062
LweI GCATC 5 cut(s) 121, 190, 413, 435, 1647
MabI ACCWGGT 1 cut(s) 582
MaeI CTAG 6 cut(s) 65, 80, 267, 785, 809, 1424
MaeII ACGT 6 cut(s) 178, 278, 343, 477, 483, 1072
MaeIII GTNAC 6 cut(s) 189, 920, 1691, 2132, 2149, 2249
MboII GAAGA 9 cut(s) 164, 326, 512, 635, 961, 1116, 1928, 2009, 2102
MfeI CAATTG 2 cut(s) 116, 1268
MflI RGATCY 5 cut(s) 848, 859, 1141, 1495, 2092
MhlI GDGCHC 3 cut(s) 1111, 1251, 1620
MlyI GAGTC 5 cut(s) 705, 779, 1391, 2040, 2288
MmeI TCCRAC 2 cut(s) 265, 723
Mph1103I ATGCAT 3 cut(s) 114, 1380, 1756
MroXI GAANNNNTTC 1 cut(s) 331
MslI CAYNNNNRTG 3 cut(s) 134, 678, 1613
MspA1I CMGCKG 1 cut(s) 2145
MspCI CTTAAG 3 cut(s) 1865, 1876, 2198
MspI CCGG 1 cut(s) 1771
MspR9I CCNGG 3 cut(s) 171, 584, 1771
MunI CAATTG 2 cut(s) 116, 1268
Mva1269I GAATGC 1 cut(s) 1598
MvaI CCWGG 2 cut(s) 171, 584
MvnI CGCG 2 cut(s) 150, 2231
MwoI GCNNNNNNNGC 3 cut(s) 1003, 1246, 1605
NciI CCSGG 1 cut(s) 1771
NcoI CCATGG 1 cut(s) 2294
NheI GCTAGC 1 cut(s) 266
NlaIV GGNNCC 1 cut(s) 898
NmuCI GTSAC 2 cut(s) 920, 2132
NruI TCGCGA 1 cut(s) 150
NsiI ATGCAT 3 cut(s) 114, 1380, 1756
NspI RCATGY 4 cut(s) 601, 623, 1357, 1758
PaeI GCATGC 1 cut(s) 1357
PagI TCATGA 2 cut(s) 828, 2002
PaqCI CACCTGC 1 cut(s) 988
PciI ACATGT 1 cut(s) 619
PcsI WCGNNNNNNNCGW 1 cut(s) 483
PctI GAATGC 1 cut(s) 1598
PdmI GAANNNNTTC 1 cut(s) 331
PfoI TCCNGGA 1 cut(s) 169
PkrI GCNGC 6 cut(s) 818, 1085, 1466, 1598, 2052, 2233
PleI GAGTC 5 cut(s) 705, 778, 1391, 2039, 2287
PpsI GAGTC 5 cut(s) 705, 778, 1391, 2039, 2287
PscI ACATGT 1 cut(s) 619
PshAI GACNNNNGTC 1 cut(s) 2130
Psp124BI GAGCTC 1 cut(s) 1251
Psp1406I AACGTT 2 cut(s) 278, 343
Psp6I CCWGG 2 cut(s) 169, 582
PspFI CCCAGC 1 cut(s) 2145
PspGI CCWGG 2 cut(s) 169, 582
PspN4I GGNNCC 1 cut(s) 898
PspPI GGNCC 4 cut(s) 247, 1531, 1861, 2177
PstNI CAGNNNCTG 1 cut(s) 401
PsuI RGATCY 5 cut(s) 848, 859, 1141, 1495, 2092
PvuII CAGCTG 1 cut(s) 2145
RruI TCGCGA 1 cut(s) 150
RsaI GTAC 2 cut(s) 1136, 2045
RsaNI GTAC 2 cut(s) 1135, 2044
RseI CAYNNNNRTG 3 cut(s) 134, 678, 1613
SacI GAGCTC 1 cut(s) 1251
SalI GTCGAC 1 cut(s) 1885
SatI GCNGC 6 cut(s) 817, 1084, 1465, 1597, 2051, 2232
Sau96I GGNCC 4 cut(s) 247, 1531, 1861, 2177
ScaI AGTACT 1 cut(s) 1136
SchI GAGTC 5 cut(s) 705, 779, 1391, 2040, 2288
ScrFI CCNGG 3 cut(s) 171, 584, 1771
SduI GDGCHC 3 cut(s) 1111, 1251, 1620
SexAI ACCWGGT 1 cut(s) 582
SfaNI GCATC 5 cut(s) 121, 190, 413, 435, 1647
SfcI CTRYAG 1 cut(s) 933
SinI GGWCC 1 cut(s) 1531
SmiMI CAYNNNNRTG 3 cut(s) 134, 678, 1613
SmlI CTYRAG 5 cut(s) 722, 1865, 1876, 2198, 2270
SmoI CTYRAG 5 cut(s) 722, 1865, 1876, 2198, 2270
SpeI ACTAGT 1 cut(s) 1423
SphI GCATGC 1 cut(s) 1357
SsiI CCGC 5 cut(s) 1596, 1968, 2130, 2231, 2284
SspI AATATT 3 cut(s) 211, 241, 1018
SspMI CTAG 6 cut(s) 65, 80, 267, 785, 809, 1424
SstI GAGCTC 1 cut(s) 1251
StyD4I CCNGG 3 cut(s) 169, 582, 1769
StyI CCWWGG 4 cut(s) 1568, 1578, 2120, 2294
TaaI ACNGT 5 cut(s) 1339, 1520, 1699, 1764, 2221
TaiI ACGT 6 cut(s) 181, 281, 346, 480, 486, 1075
TaqI TCGA 8 cut(s) 98, 486, 1503, 1674, 1783, 1886, 2190, 2265
TatI WGTACW 1 cut(s) 1134
TauI GCSGC 2 cut(s) 1599, 2234
TscAI CASTG 1 cut(s) 1702
TseFI GTSAC 2 cut(s) 920, 2132
TseI GCWGC 4 cut(s) 816, 1083, 1464, 2050
Tsp45I GTSAC 2 cut(s) 920, 2132
TspDTI ATGAA 7 cut(s) 845, 962, 1196, 1925, 1971, 2148, 2271
TspGWI ACGGA 2 cut(s) 248, 2168
TspRI CASTG 1 cut(s) 1702
Vha464I CTTAAG 3 cut(s) 1865, 1876, 2198
VneI GTGCAC 1 cut(s) 1616
VpaK11BI GGWCC 1 cut(s) 1531
XagI CCTNNNNNAGG 1 cut(s) 2201
XapI RAATTY 8 cut(s) 87, 459, 503, 565, 657, 751, 1309, 1402
XbaI TCTAGA 1 cut(s) 808
XceI RCATGY 4 cut(s) 601, 623, 1357, 1758
XmiI GTMKAC 2 cut(s) 1759, 1886
XmnI GAANNNNTTC 1 cut(s) 331
XspI CTAG 6 cut(s) 65, 80, 267, 785, 809, 1424
ZrmI AGTACT 1 cut(s) 1136
Zsp2I ATGCAT 3 cut(s) 114, 1380, 1756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.