pycom07g06290

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000654
Physical Location & Seq
Forward (+)
5558798 .. 5563181
4384 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g06290.2

Sequence Viewer

Length: 3633 bp
ATGGCTGGTGCTATAAGCTCCATGCAGCTGCTCGACACTTCAAGCTCTTACCGATGTACTTATCATGTGTTCTTGAGTTTCAGAGGCGAAGATACGCGTAAGAGCTTTACTGATCACCTCTACCGGGCTTTGGAGTTGGCAGGAATCCACACCTTTAGAGACGATGATGAAATCGAGAGAGGAGAAGAGATAGCAGAAGAGTTACATAAAGCTATAAATGAGTCAAAAGTATCGATAATTGTCTTCTCCAAGAACTATGCTTCCTCGCGGTGGTGCTTGGACGAACTTGTTAAGATCATGGAACGTAGAAGAAGTGATGATGAGCATTTTGTTTTGCCGGTTTTCTATGATGTGGATCCATCCCATGTGCGGAAGCAGACTGGAAGTTTTGAAGAATCGTTTCATAGACATAAAGAGCGCTTCAAGGAGGAGATGGACAAGGTGGACGGGTGGAGAAGAGCACTTAGAGATGTTGCAGATCTGGCAGGGATGGTTTTGGGAAATAGGTACGAGTCGCAGTTTATCCAAGATATTGTTAAAGAGGTTGGAAATAAATTGGATCCCAAAGCATTGAACGTTGCACCGTATCCTGTTGGCATAGAAGATCGTGTGCGAGGCATAAACATGTGGTTACAAGACGGATCAAATGATGTTGGTGTAGCTGTGATATGTGGAATGGGTGGAATTGGCAAGTCCACCATTGCCAGAGCTGCCTATAACCTCAACTGTGCAAGATTCCAAGGTAGCAGCTTTCTTGCAGATATTAGAGCAAGTTCAGAACAGCCATATGGTCTCGTTCGCTTGCAAAGGAAACTTCTTTCGGACATCCAAAAAGGGAAAGCAAATAAAATATACGACATAAATGAAGGGATAATGAAGATCAAACATGCTGTCTGTAACAAGAGAGTTCTTATTGTTCTTGACGATGTGAATGACTTGGATCAATTTAACGCAATTCTTGGAATGCGGGAATGGTTTTATCCAGGAAGTAAAATCATCATAACAACCAGGCACGAGCATTTGTTGAAGGCACATGAAGATTATACAATGTTCGAGGTTGAAGAATTGAATGAGAATGAATCGCTTGAGCTTTTCAGTTGGCATGCATTCGGACAAGCCCAACCTGTTGAAGGTTACATGGAACTTTCGAGACCAGCAGCGCAACACTGTGAAGGGCTACCATTAGCTCTTCAAGTTTTGGGATCCTCTCTATCTGGGAAACTAGTAGATGTATGGAAAAGTGCTTTACAGAAGTTATGCGAAATTCCCAATGACAAAGTTCACAAAGTTCTTCGAGTAAGCTTCGATTCTCTACAAGATGATCATGACAAAAATATATTCCTCTATGTAGCCTGTTTCTTCATTGGAAAGGAGAAGGATTTTGCAATTACAGTACTGGACAACCTTGACTTTTGCACGAGGATTGGAATTCAAAATCTGGTGGACAGATGTCTAGTAAAAATCGATAATGAAGACAACAGGCTGGCTATGCATCACTTACTTAGAGACATGGGAAGGGGAATTATTCGGGAAGAATCACCTCAAGATCCTGGAAAGCGTAGTATAGTGTGGCATAAAGATTCCTTTAATATTTTGAGAAAAAGAACGGGTACGGCAACTGTTAAAGGCCTCATGCTCAACCTGCCTGTGTTGATGGAAGATGAGTCTTTGAAGCCATTATTTGGTAAAAGTAACAAAAAACGATGCAATGTTGAAGATTATGAAGGAAATTTTTCAAGACGTCGTCGGCTTGATTTCTTTTCTTGGAAATCAATTGCAAGTAACTTTTCTTCAACATACTCAACTCCTGCATCAAATGATTTGAAGTTCAGAACTGACGCATTTACAATGATGAACAATCTTGAACTGCTCGTGCTCAATAATGTAAGCATCTGTGGAGGCTTCGAAGATTTTCCAAAACATTTGACATGGTTGTCTTGGAGAGGGTTTCCTTTAAAATGGATACCAGCCATTTTTTGTATGGAAAATCTAGTTATTCTTGACTTGCGGAATAGCAGCCTGCAACATGTTTGGAAGGGAACCAGGTTTGTTGGTAGAATGAAAGTTCTTAATCTCAGTCATTCACACAGCCTTTTGACAACCCCTGACATGTCGGGACTTCCTAACCTTGAGAGATTAATTCTTAAAGATTGTATAAGTTTGGTTGAGGTTGATGAGTCTATTGGAGACTTGGAGAAACTTGTTGTTTTGAATCTAAGAGACTGCAAAAATCTCAGTAGGATTCCAACAAGTTTTAATAGGTCGGGATCTCTTCAAGTTCTCATTCTTTCTGGTTGCTCCAAGCTGGGTAATGCAACAGAGCAGGTCTACTCAGGAGCTAGTGTTATGAAGAAATTCAATATGTTATCAGCTAACTTATGGCAATCATTAACGTCGTGGGTATTACCAAGAAAAAATCTTGCATTGACCAGTTTCTCATTCAAAAGTTTTCCACATTCTTTGGGAAGATTAAGTCTTGCCAACTGCAATCTATCAGAGATTTCTAGTGATCTTGGTGCCCTATCGGTGTTGAAGCATTTGGATCTATCTGCAAACCCAATTCCAAACCTACCAGAAAACATGAACGGTCTTCACATGCTCCAAGAACTGCTAGTAGAGGATTGCACAAAGCTCCAAACACTTCCGGAACTTCCACCAAAATTAAAGACATTGGAAGCAGGTCGGTGTAAATCATTGAAAACAGTAACAAATTTACCAAACGCCTTTGAATCATTGAGTAAAAATTTTGATGAATGTGAGAAGTTAGTTGAAATTCAAAGCTTGTTCGAAATAAAACCGTTGAGAAACGTTGACATAGAAATGATCAAAGATGTGGGCCTGTTTAACTTGGAATCCACTGAAAGCACCGAAGTGGAAATGATCAACTACCTGACATGTACAACCAAGAAGTGTTGCCTTCAGGTACTGAAAGAATGCGGCATATTTAGCATTTTCATTCATGGGAGCAAGATTCCAGATTGGTTCAGCTGCAGGAGCATGGGTAACTCGTTGTTGTCGATTTTCGTACCTTTAAATGTTAATATCAAGGTCCGAGGCTTAAATGCATGCGTTATATACACACGACTCCCTGATCGAAGCGGTGGTGTACATGTTGCCAGTGAACACTTTCTCAAAGTGAGTAATGAGACCAAGGGTCGTATGTGGACCTATTCCCCTGTCGCAATGGGTCTTCCAAAAGAGAACGAAGACATGTTATGGCTAAGTCATTGGGTGTTTGGAAATGATGAACTGGAGAGTGGGGACGAAATACGGGTTTCTGTGAAGTGCGGTTTGTGTGTAAAAGAGTTTGGAATCGAACTTGTGTACGAGGACGAAAGTAAGGGTGAGGGTGTTCGATCAAATAATGAAGATACAACACAAAGTAATGTTGTTGCAGGAAATGTCACAGTGTCGGATTCAATGTACGAAATGTGGAGAGGCAAGTACTTTCTTTGTAATCATAGGCTTCGAACCCATCAAGCTCAATTCAGAAGGAGACAAGAGAACCCAGCTTTTGCTGATTTTTCATACAAGCCAGGGGACTCCTTTTATTTGAATGGTTTCTTATTCAAGCACGAGGTTGACATTGCGAAGTACCAACTGCAGTATTTTAGAAGAGCATTGACTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1211

Amino Acids

137.77

Weight (kDa)

6.58

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1933
AatII GACGTC 1 cut(s) 1744
Acc36I ACCTGC 3 cut(s) 1650, 2314, 2669
AccB1I GGYRCC 1 cut(s) 2516
AccB7I CCANNNNNTGG 1 cut(s) 1682
AccI GTMKAC 1 cut(s) 2328
AccII CGCG 2 cut(s) 97, 268
AccIII TCCGGA 1 cut(s) 2644
AciI CCGC 7 cut(s) 268, 370, 967, 2008, 2937, 3099, 3288
AclI AACGTT 2 cut(s) 576, 2808
AcsI RAATTY 7 cut(s) 1263, 1428, 1729, 2354, 2710, 2743, 2772
AcuI CTGAAG 1 cut(s) 2903
AcyI GRCGYC 1 cut(s) 1741
AfeI AGCGCT 1 cut(s) 419
AfiI CCNNNNNNNGG 6 cut(s) 124, 130, 270, 369, 1130, 1682
AflIII ACRYGT 7 cut(s) 95, 624, 2026, 2109, 2894, 3109, 3210
AhdI GACNNNNNGTC 1 cut(s) 3153
AhlI ACTAGT 1 cut(s) 1222
AjnI CCWGG 5 cut(s) 982, 1007, 1549, 2042, 3535
AleI CACNNNNGTG 1 cut(s) 2870
AloI GAACNNNNNNTCC 2 cut(s) 245, 277
Alw21I GWGCWC 2 cut(s) 463, 1878
Alw26I GTCTC 8 cut(s) 153, 797, 1144, 1500, 2181, 2214, 3140, 3490
AlwNI CAGNNNCTG 1 cut(s) 2926
Aor13HI TCCGGA 1 cut(s) 2644
Aor51HI AGCGCT 1 cut(s) 419
AoxI GGCC 2 cut(s) 1627, 2836
ApeKI GCWGC 7 cut(s) 25, 28, 710, 747, 1157, 2016, 2988
ApoI RAATTY 7 cut(s) 1263, 1428, 1729, 2354, 2710, 2743, 2772
ArsI GACNNNNNNTTYG 2 cut(s) 876, 908
AseI ATTAAT 1 cut(s) 2138
Asp700I GAANNNNTTC 6 cut(s) 399, 1732, 1911, 2354, 3126, 3560
AspLEI GCGC 2 cut(s) 420, 1162
AspS9I GGNCC 3 cut(s) 2836, 3049, 3165
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 3 cut(s) 107, 1530, 3356
AsuII TTCGAA 3 cut(s) 1905, 2787, 3469
AvaII GGWCC 2 cut(s) 3049, 3165
BaeGI GKGCMC 1 cut(s) 2521
BamHI GGATCC 3 cut(s) 355, 559, 1202
BanI GGYRCC 1 cut(s) 2516
BarI GAAGNNNNNNTAC 2 cut(s) 1774, 1806
BauI CACGAG 4 cut(s) 1013, 1417, 1871, 3575
BbsI GAAGAC 5 cut(s) 235, 1479, 2582, 3182, 3213
Bbv12I GWGCWC 2 cut(s) 463, 1878
BbvI GCAGC 7 cut(s) 15, 37, 697, 759, 1169, 2028, 2975
BccI CCATC 5 cut(s) 367, 427, 484, 1648, 3483
BceAI ACGGC 1 cut(s) 1629
BciT130I CCWGG 5 cut(s) 984, 1009, 1551, 2044, 3537
BciVI GTATCC 2 cut(s) 597, 1956
BclI TGATCA 4 cut(s) 112, 1321, 2823, 2880
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 8 cut(s) 153, 797, 1144, 1500, 2181, 2214, 3140, 3490
BcuI ACTAGT 1 cut(s) 1222
BfaI CTAG 6 cut(s) 1223, 1454, 1991, 2340, 2505, 2612
BfmI CTRYAG 2 cut(s) 2989, 3602
BfoI RGCGCY 1 cut(s) 421
BfuAI ACCTGC 3 cut(s) 1650, 2314, 2669
BfuI GTATCC 2 cut(s) 597, 1956
BglII AGATCT 1 cut(s) 478
BisI GCNGC 8 cut(s) 26, 29, 711, 748, 1158, 2017, 2938, 2989
BlsI GCNGC 8 cut(s) 27, 30, 712, 749, 1159, 2018, 2939, 2990
BmcAI AGTACT 2 cut(s) 1395, 3446
Bme1390I CCNGG 6 cut(s) 125, 984, 1009, 1551, 2044, 3537
Bme18I GGWCC 2 cut(s) 3049, 3165
BmeRI GACNNNNNGTC 1 cut(s) 3153
BmgT120I GGNCC 3 cut(s) 2836, 3049, 3165
BmiI GGNNCC 5 cut(s) 357, 561, 1204, 2041, 2518
BmrFI CCNGG 6 cut(s) 125, 984, 1009, 1551, 2044, 3537
BmsI GCATC 4 cut(s) 1501, 1694, 1820, 1899
BoxI GACNNNNGTC 1 cut(s) 1449
BpiI GAAGAC 5 cut(s) 235, 1479, 2582, 3182, 3213
BpmI CTGGAG 1 cut(s) 3272
Bpu14I TTCGAA 3 cut(s) 1905, 2787, 3469
BpuEI CTTGAG 4 cut(s) 94, 1106, 1527, 2150
BpuMI CCSGG 1 cut(s) 125
Bsa29I ATCGAT 2 cut(s) 233, 1464
BsaBI GATNNNNATC 1 cut(s) 354
BsaHI GRCGYC 1 cut(s) 1741
BsaI GGTCTC 3 cut(s) 797, 1144, 3140
BsaJI CCNNGG 4 cut(s) 739, 3052, 3150, 3536
BsaWI WCCGGW 1 cut(s) 2644
BsaXI ACNNNNNCTCC 4 cut(s) 2328, 2358, 2986, 3016
Bsc4I CCNNNNNNNGG 6 cut(s) 124, 130, 270, 369, 1130, 1682
Bse118I RCCGGY 1 cut(s) 337
Bse1I ACTGG 5 cut(s) 385, 1401, 2430, 3117, 3255
Bse3DI GCAATG 4 cut(s) 699, 1714, 3189, 3585
Bse8I GATNNNNATC 1 cut(s) 354
BseAI TCCGGA 1 cut(s) 2644
BseBI CCWGG 5 cut(s) 984, 1009, 1551, 2044, 3537
BseCI ATCGAT 2 cut(s) 233, 1464
BseDI CCNNGG 4 cut(s) 739, 3052, 3150, 3536
BseGI GGATG 3 cut(s) 359, 495, 825
BseJI GATNNNNATC 1 cut(s) 354
BseLI CCNNNNNNNGG 6 cut(s) 124, 130, 270, 369, 1130, 1682
BseMI GCAATG 4 cut(s) 699, 1714, 3189, 3585
BseMII CTCAG 3 cut(s) 2089, 2248, 2346
BseNI ACTGG 5 cut(s) 385, 1401, 2430, 3117, 3255
BseRI GAGGAG 2 cut(s) 195, 443
BseSI GKGCMC 1 cut(s) 2521
BseXI GCAGC 7 cut(s) 15, 37, 697, 759, 1169, 2028, 2975
BseYI CCCAGC 2 cut(s) 2305, 3508
Bsh1236I CGCG 2 cut(s) 97, 268
BshFI GGCC 2 cut(s) 1629, 2838
BshNI GGYRCC 1 cut(s) 2516
BshVI ATCGAT 2 cut(s) 233, 1464
BsiHKAI GWGCWC 2 cut(s) 463, 1878
BsiSI CCGG 3 cut(s) 124, 338, 2645
BslFI GGGAC 3 cut(s) 2130, 3275, 3554
BslI CCNNNNNNNGG 6 cut(s) 124, 130, 270, 369, 1130, 1682
BsmAI GTCTC 8 cut(s) 153, 797, 1144, 1500, 2181, 2214, 3140, 3490
BsmBI CGTCTC 1 cut(s) 153
BsmFI GGGAC 3 cut(s) 2130, 3275, 3554
BsmI GAATGC 3 cut(s) 969, 1106, 2939
BsnI GGCC 2 cut(s) 1629, 2838
Bso31I GGTCTC 3 cut(s) 797, 1144, 3140
Bsp119I TTCGAA 3 cut(s) 1905, 2787, 3469
Bsp1286I GDGCHC 3 cut(s) 463, 1878, 2521
Bsp13I TCCGGA 1 cut(s) 2644
Bsp1407I TGTACA 2 cut(s) 2897, 3106
BspACI CCGC 7 cut(s) 268, 370, 967, 2008, 2937, 3099, 3288
BspANI GGCC 2 cut(s) 1629, 2838
BspCNI CTCAG 3 cut(s) 2088, 2247, 2345
BspDI ATCGAT 2 cut(s) 233, 1464
BspEI TCCGGA 1 cut(s) 2644
BspFNI CGCG 2 cut(s) 97, 268
BspHI TCATGA 1 cut(s) 1324
BspLI GGNNCC 5 cut(s) 357, 561, 1204, 2041, 2518
BspMAI CTGCAG 2 cut(s) 2993, 3606
BspMI ACCTGC 3 cut(s) 1650, 2314, 2669
BspQI GCTCTTC 3 cut(s) 451, 1194, 3610
BspT104I TTCGAA 3 cut(s) 1905, 2787, 3469
BspT107I GGYRCC 1 cut(s) 2516
BspTNI GGTCTC 3 cut(s) 797, 1144, 3140
BsrDI GCAATG 4 cut(s) 699, 1714, 3189, 3585
BsrFI RCCGGY 1 cut(s) 337
BsrGI TGTACA 2 cut(s) 2897, 3106
BsrI ACTGG 5 cut(s) 385, 1401, 2430, 3117, 3255
BssAI RCCGGY 1 cut(s) 337
BssECI CCNNGG 4 cut(s) 739, 3052, 3150, 3536
BssNI GRCGYC 1 cut(s) 1741
BssSI CACGAG 4 cut(s) 1013, 1417, 1871, 3575
BssT1I CCWWGG 2 cut(s) 739, 3150
Bst2BI CACGAG 4 cut(s) 1013, 1417, 1871, 3575
Bst2UI CCWGG 5 cut(s) 984, 1009, 1551, 2044, 3537
Bst4CI ACNGT 9 cut(s) 585, 728, 1169, 1393, 1621, 2588, 2704, 2799, 3409
Bst6I CTCTTC 6 cut(s) 180, 192, 451, 1194, 2277, 3610
BstACI GRCGYC 1 cut(s) 1741
BstAUI TGTACA 2 cut(s) 2897, 3106
BstBI TTCGAA 3 cut(s) 1905, 2787, 3469
BstC8I GCNNGC 5 cut(s) 803, 1104, 1485, 2021, 3067
BstDEI CTNAG 7 cut(s) 464, 1502, 2075, 2216, 2234, 2332, 3221
BstENI CCTNNNNNAGG 1 cut(s) 1128
BstF5I GGATG 3 cut(s) 359, 495, 825
BstFNI CGCG 2 cut(s) 97, 268
BstH2I RGCGCY 1 cut(s) 421
BstHHI GCGC 2 cut(s) 420, 1162
BstMAI GTCTC 8 cut(s) 153, 797, 1144, 1500, 2181, 2214, 3140, 3490
BstMWI GCNNNNNNNGC 6 cut(s) 482, 710, 1489, 1642, 2946, 2994
BstNI CCWGG 5 cut(s) 984, 1009, 1551, 2044, 3537
BstPAI GACNNNNGTC 1 cut(s) 1449
BstSCI CCNGG 6 cut(s) 123, 982, 1007, 1549, 2042, 3535
BstSFI CTRYAG 2 cut(s) 2989, 3602
BstSLI GKGCMC 1 cut(s) 2521
BstUI CGCG 2 cut(s) 97, 268
BstV1I GCAGC 7 cut(s) 15, 37, 697, 759, 1169, 2028, 2975
BstV2I GAAGAC 5 cut(s) 235, 1479, 2582, 3182, 3213
BstX2I RGATCY 7 cut(s) 355, 478, 559, 1202, 1546, 2267, 2542
BstYI RGATCY 7 cut(s) 355, 478, 559, 1202, 1546, 2267, 2542
Bsu15I ATCGAT 2 cut(s) 233, 1464
BsuI GTATCC 2 cut(s) 597, 1956
BsuRI GGCC 2 cut(s) 1629, 2838
BsuTUI ATCGAT 2 cut(s) 233, 1464
BtsCI GGATG 3 cut(s) 359, 495, 825
BtsIMutI CAGTG 4 cut(s) 1165, 2856, 3124, 3414
BveI ACCTGC 3 cut(s) 1650, 2314, 2669
Cac8I GCNNGC 5 cut(s) 803, 1104, 1485, 2021, 3067
CaiI CAGNNNCTG 1 cut(s) 2926
CciI TCATGA 1 cut(s) 1324
CfoI GCGC 2 cut(s) 420, 1162
Cfr10I RCCGGY 1 cut(s) 337
Cfr13I GGNCC 3 cut(s) 2836, 3049, 3165
ClaI ATCGAT 2 cut(s) 233, 1464
CseI GACGC 1 cut(s) 1847
CsiI ACCWGGT 1 cut(s) 2042
CspCI CAANNNNNGTGG 2 cut(s) 2442, 2477
DdeI CTNAG 7 cut(s) 464, 1502, 2075, 2216, 2234, 2332, 3221
DraI TTTAAA 2 cut(s) 1956, 3033
DrdI GACNNNNNNGTC 1 cut(s) 1933
DriI GACNNNNNGTC 1 cut(s) 3153
DseDI GACNNNNNNGTC 1 cut(s) 1933
Eam1104I CTCTTC 6 cut(s) 180, 192, 451, 1194, 2277, 3610
Eam1105I GACNNNNNGTC 1 cut(s) 3153
EarI CTCTTC 6 cut(s) 180, 192, 451, 1194, 2277, 3610
Eco130I CCWWGG 2 cut(s) 739, 3150
Eco147I AGGCCT 1 cut(s) 1629
Eco31I GGTCTC 3 cut(s) 797, 1144, 3140
Eco47I GGWCC 2 cut(s) 3049, 3165
Eco47III AGCGCT 1 cut(s) 419
Eco57I CTGAAG 1 cut(s) 2903
EcoNI CCTNNNNNAGG 1 cut(s) 1128
EcoRI GAATTC 1 cut(s) 1428
EcoRII CCWGG 5 cut(s) 982, 1007, 1549, 2042, 3535
EcoT14I CCWWGG 2 cut(s) 739, 3150
EcoT22I ATGCAT 3 cut(s) 1108, 1494, 3067
ErhI CCWWGG 2 cut(s) 739, 3150
Esp3I CGTCTC 1 cut(s) 153
FaqI GGGAC 3 cut(s) 2130, 3275, 3554
FauI CCCGC 1 cut(s) 960
FauNDI CATATG 1 cut(s) 787
FbaI TGATCA 4 cut(s) 112, 1321, 2823, 2880
FblI GTMKAC 1 cut(s) 2328
Fnu4HI GCNGC 8 cut(s) 26, 29, 711, 748, 1158, 2017, 2938, 2989
FokI GGATG 3 cut(s) 346, 502, 812
Fsp4HI GCNGC 8 cut(s) 26, 29, 711, 748, 1158, 2017, 2938, 2989
FspBI CTAG 6 cut(s) 1223, 1454, 1991, 2340, 2505, 2612
GlaI GCGC 2 cut(s) 419, 1161
GluI GCNGC 8 cut(s) 26, 29, 711, 748, 1158, 2017, 2938, 2989
GsaI CCCAGC 2 cut(s) 2309, 3512
GsuI CTGGAG 1 cut(s) 3272
HaeII RGCGCY 1 cut(s) 421
HaeIII GGCC 2 cut(s) 1629, 2838
HapII CCGG 3 cut(s) 124, 338, 2645
HgaI GACGC 1 cut(s) 1847
HhaI GCGC 2 cut(s) 420, 1162
Hin1I GRCGYC 1 cut(s) 1741
Hin6I GCGC 2 cut(s) 418, 1160
HinP1I GCGC 2 cut(s) 418, 1160
HincII GTYRAC 2 cut(s) 2812, 3583
HindII GTYRAC 2 cut(s) 2812, 3583
HindIII AAGCTT 2 cut(s) 1300, 2779
HpaII CCGG 3 cut(s) 124, 338, 2645
HphI GGTGA 3 cut(s) 107, 1530, 3356
Hpy188I TCNGA 9 cut(s) 83, 778, 823, 1112, 1832, 2497, 3053, 3415, 3491
Hpy99I CGWCG 3 cut(s) 1746, 1749, 2398
HpyCH4III ACNGT 9 cut(s) 585, 728, 1169, 1393, 1621, 2588, 2704, 2799, 3409
HpyCH4IV ACGT 5 cut(s) 304, 576, 1741, 2393, 2808
HpyF10VI GCNNNNNNNGC 6 cut(s) 482, 710, 1489, 1642, 2946, 2994
HpyF3I CTNAG 7 cut(s) 464, 1502, 2075, 2216, 2234, 2332, 3221
HpySE526I ACGT 5 cut(s) 304, 576, 1741, 2393, 2808
Hsp92I GRCGYC 1 cut(s) 1741
HspAI GCGC 2 cut(s) 418, 1160
Kpn2I TCCGGA 1 cut(s) 2644
Ksp22I TGATCA 4 cut(s) 112, 1321, 2823, 2880
LguI GCTCTTC 3 cut(s) 451, 1194, 3610
LmnI GCTCC 7 cut(s) 23, 2303, 2336, 2604, 2637, 2964, 2994
Lsp1109I GCAGC 7 cut(s) 15, 37, 697, 759, 1169, 2028, 2975
LweI GCATC 4 cut(s) 1501, 1694, 1820, 1899
MabI ACCWGGT 1 cut(s) 2042
MaeI CTAG 6 cut(s) 1223, 1454, 1991, 2340, 2505, 2612
MaeII ACGT 5 cut(s) 304, 576, 1741, 2393, 2808
MaeIII GTNAC 9 cut(s) 201, 630, 896, 1133, 1691, 1781, 2704, 3002, 3403
MfeI CAATTG 1 cut(s) 1773
MflI RGATCY 7 cut(s) 355, 478, 559, 1202, 1546, 2267, 2542
MhlI GDGCHC 3 cut(s) 463, 1878, 2521
MluI ACGCGT 1 cut(s) 95
MlyI GAGTC 6 cut(s) 230, 521, 1673, 2186, 3078, 3536
MmeI TCCRAC 3 cut(s) 526, 2270, 3393
Mph1103I ATGCAT 3 cut(s) 1108, 1494, 3067
MroI TCCGGA 1 cut(s) 2644
MroXI GAANNNNTTC 6 cut(s) 399, 1732, 1911, 2354, 3126, 3560
MslI CAYNNNNRTG 3 cut(s) 623, 2819, 2870
MspA1I CMGCKG 2 cut(s) 28, 2988
MspI CCGG 3 cut(s) 124, 338, 2645
MspR9I CCNGG 6 cut(s) 125, 984, 1009, 1551, 2044, 3537
MunI CAATTG 1 cut(s) 1773
Mva1269I GAATGC 3 cut(s) 969, 1106, 2939
MvaI CCWGG 5 cut(s) 984, 1009, 1551, 2044, 3537
MvnI CGCG 2 cut(s) 97, 268
MwoI GCNNNNNNNGC 6 cut(s) 482, 710, 1489, 1642, 2946, 2994
NciI CCSGG 1 cut(s) 125
NdeI CATATG 1 cut(s) 787
NlaIV GGNNCC 5 cut(s) 357, 561, 1204, 2041, 2518
NmuCI GTSAC 1 cut(s) 3403
NsiI ATGCAT 3 cut(s) 1108, 1494, 3067
NspV TTCGAA 3 cut(s) 1905, 2787, 3469
OliI CACNNNNGTG 1 cut(s) 2870
PaeI GCATGC 2 cut(s) 1106, 3069
PagI TCATGA 1 cut(s) 1324
PceI AGGCCT 1 cut(s) 1629
PciI ACATGT 6 cut(s) 624, 2026, 2109, 2894, 3109, 3210
PciSI GCTCTTC 3 cut(s) 451, 1194, 3610
PcsI WCGNNNNNNNCGW 1 cut(s) 3014
PctI GAATGC 3 cut(s) 969, 1106, 2939
PdmI GAANNNNTTC 6 cut(s) 399, 1732, 1911, 2354, 3126, 3560
PflFI GACNNNGTC 1 cut(s) 1743
PflMI CCANNNNNTGG 1 cut(s) 1682
PfoI TCCNGGA 2 cut(s) 982, 1549
PkrI GCNGC 8 cut(s) 27, 30, 712, 749, 1159, 2018, 2939, 2990
PleI GAGTC 6 cut(s) 229, 520, 1672, 2185, 3078, 3536
PpsI GAGTC 6 cut(s) 229, 520, 1672, 2185, 3078, 3536
PscI ACATGT 6 cut(s) 624, 2026, 2109, 2894, 3109, 3210
PshAI GACNNNNGTC 1 cut(s) 1449
PshBI ATTAAT 1 cut(s) 2138
Psp1406I AACGTT 2 cut(s) 576, 2808
Psp6I CCWGG 5 cut(s) 982, 1007, 1549, 2042, 3535
PspFI CCCAGC 2 cut(s) 2305, 3508
PspGI CCWGG 5 cut(s) 982, 1007, 1549, 2042, 3535
PspN4I GGNNCC 5 cut(s) 357, 561, 1204, 2041, 2518
PspPI GGNCC 3 cut(s) 2836, 3049, 3165
PstI CTGCAG 2 cut(s) 2993, 3606
PstNI CAGNNNCTG 1 cut(s) 2926
PsuI RGATCY 7 cut(s) 355, 478, 559, 1202, 1546, 2267, 2542
PsyI GACNNNGTC 1 cut(s) 1743
PvuII CAGCTG 2 cut(s) 28, 2988
RseI CAYNNNNRTG 3 cut(s) 623, 2819, 2870
SapI GCTCTTC 3 cut(s) 451, 1194, 3610
SatI GCNGC 8 cut(s) 26, 29, 711, 748, 1158, 2017, 2938, 2989
Sau96I GGNCC 3 cut(s) 2836, 3049, 3165
ScaI AGTACT 2 cut(s) 1395, 3446
SchI GAGTC 6 cut(s) 230, 521, 1673, 2186, 3078, 3536
ScrFI CCNGG 6 cut(s) 125, 984, 1009, 1551, 2044, 3537
SduI GDGCHC 3 cut(s) 463, 1878, 2521
SexAI ACCWGGT 1 cut(s) 2042
SfaNI GCATC 4 cut(s) 1501, 1694, 1820, 1899
SfcI CTRYAG 2 cut(s) 2989, 3602
SfuI TTCGAA 3 cut(s) 1905, 2787, 3469
SinI GGWCC 2 cut(s) 3049, 3165
SmiMI CAYNNNNRTG 3 cut(s) 623, 2819, 2870
SmlI CTYRAG 4 cut(s) 73, 1085, 1542, 2129
SmoI CTYRAG 4 cut(s) 73, 1085, 1542, 2129
SpeI ACTAGT 1 cut(s) 1222
SphI GCATGC 2 cut(s) 1106, 3069
SseBI AGGCCT 1 cut(s) 1629
SsiI CCGC 7 cut(s) 268, 370, 967, 2008, 2937, 3099, 3288
SspI AATATT 1 cut(s) 1591
SspMI CTAG 6 cut(s) 1223, 1454, 1991, 2340, 2505, 2612
StuI AGGCCT 1 cut(s) 1629
StyD4I CCNGG 6 cut(s) 123, 982, 1007, 1549, 2042, 3535
StyI CCWWGG 2 cut(s) 739, 3150
TaaI ACNGT 9 cut(s) 585, 728, 1169, 1393, 1621, 2588, 2704, 2799, 3409
TaiI ACGT 5 cut(s) 307, 579, 1744, 2396, 2811
TatI WGTACW 5 cut(s) 56, 1393, 2897, 3106, 3444
TauI GCSGC 1 cut(s) 2940
TscAI CASTG 4 cut(s) 1172, 2863, 3124, 3414
TseFI GTSAC 1 cut(s) 3403
TseI GCWGC 7 cut(s) 25, 28, 710, 747, 1157, 2016, 2988
Tsp45I GTSAC 1 cut(s) 3403
TspGWI ACGGA 1 cut(s) 654
TspRI CASTG 4 cut(s) 1172, 2863, 3124, 3414
Tth111I GACNNNGTC 1 cut(s) 1743
Van91I CCANNNNNTGG 1 cut(s) 1682
VpaK11BI GGWCC 2 cut(s) 3049, 3165
VspI ATTAAT 1 cut(s) 2138
XagI CCTNNNNNAGG 1 cut(s) 1128
XapI RAATTY 7 cut(s) 1263, 1428, 1729, 2354, 2710, 2743, 2772
XcmI CCANNNNNNNNNTGG 1 cut(s) 1978
XmiI GTMKAC 1 cut(s) 2328
XmnI GAANNNNTTC 6 cut(s) 399, 1732, 1911, 2354, 3126, 3560
XspI CTAG 6 cut(s) 1223, 1454, 1991, 2340, 2505, 2612
ZraI GACGTC 1 cut(s) 1742
ZrmI AGTACT 2 cut(s) 1395, 3446
Zsp2I ATGCAT 3 cut(s) 1108, 1494, 3067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.