Prupe.3G009700_v2.0.a1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000654
Physical Location & Seq
Forward (+)
624864 .. 629126
4263 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G009700.1

Sequence Viewer

Length: 3528 bp
ATGGCTGCCACCAGCACGCAGCAGAATGCTTCTCCGTCCTCTCACCGGTGCACTTATGATGCATTCTTGAGCTTCAGGGGCAGAGACACACGCAAGGGATTTGCTGATCACCTCTACAGGGCTTTGGAGGTGGCAGGGATCCACACTTTTAGAGATGATGACGAAATCGAGAGAGGAGCAAATATTTTAGCAGAGCTTCAAAAAGCAATACAAGAGTCTCGAGTATCCATCATTGTCTTCTCCAAGGACTACGCTTCCTCGAGATGGTGCCTAGACGAACTAGTGATGATCATGGACCGTAGAGAAACTAATGGGCACATGGTTATGCCGATTTTCTATGACGTGGATCCATCCCATGTCAGGAACCAGACAGGAATTTTTGAAGAAGCATTTTCCAGACACCAACAGCGCTTCAACAAGGAGATGGACAAGGTGGAGAAGTGGAGAAAAGCTCTCAGAGATGTTGCAGATTTGGGAGGGATGGTCTTAGGAGATCGGTACGAGTCGCAGTTTATCCAAGATATAGTTGAAGTTATTGGAAATAAACTGGATCACACATGGAATAGGAGATTAAGAGTCGATCCCTATTTAGTTGGAATAGATAATCGTGTGGAAGGCCTAAACATGTGGTTAGAAGATGGATCAAGTGATGTTGGAGTAGCTGTAGTCTATGGGATGGGTGGAATTGGCAAGACCACCATTGCCAAGACTGCTTATAACCAGAACTGCAATAAATTTCAAGGTAGCAGCTTTCTTGCAGATATTAGAGCAACTTCAAAACTTCCCAATGGTTTTGTACACTTGCAAAGGAACCTTCTTTCAGATCTCCAAAAGGGGAAAGCAAAGAAAATATACAGCTTGGATGAAGGAATAACAAAGATCAAACGGGCGATACGTTGCAAAAGAGTTCTTATTGCTCTTGATGATGTGGACAACTTGGAACAATTCAATGCAATTCTTGGAATGCGAGAATGGCTTCATCCAGGAAGTAAAATAATCATAACAACTAGACATGAACATTTGTTAAAGGCTCATGAGAATTGTGCAATGTTTAATGTGGAAGGGTTGCTTGAGAATGAATCACTTGAGCTTTTCAGTTGGCATGCCTTTAGACAACCCCATCCTGGCGAAGGTTACATGGATCTTTCAAGACCTGTAGTGCAACACTGTGGAGGGGTTCCATTAGCTCTTAAAGTTTTAGGATCTGCTCTCTTTGGAAAGCTGCAGATGTATGGGAAAAATGCATTACAGAACTTGGATGTGATTACTGAAGGAAAAATTGAAAAGATTCTCAGAGTAAGCTTTGATTCTCTGCAAGATCATGACAAACGTCTATTCCTCCATATAGCCTGTTTCTTCATAGGAAGACACAAGGATTTTTCAATTACAGTCCTTGATGAATGTGGGTTTGCCACAAACATTGGAATTCAAAATCTGGTTGACAGATGTCTGCTGATAATTGATGGATTCAACAAGTTAACCATGCACCAATTACTTCAAGACATGGGAAGGGGAATTATTCGCGAAGAATCGCCTGAGGATCCTGGAAAACGTACTAGAGTGTGGAATAAAGATGCCTCTAATGTTTTGAGAAAATTAACTGGTACAGCAACTATTAAGGGCCTCATGCTCAACATTCCTATGTTAATAAAAGATGAGTCTTCTAAGATAATATCCAGTGGAAGTAACAGAAAAAGATTCCATGTTGAAGATTATGATGGAAACTGTTCAAGCAGCCGACGTCGGCTTGGTTTCTTCTCCTGGCAGTCAATTACAAACTCATTTCCTGTATCAAATGAGATAGGTTTCAAAACAGAGGGATTTAGAAGCATGCACAATCTTGAACTCCTGTTGCTTGATAATGTAAAAATCAGTGGAGGCTATGAAGATTTCCCAACAAATTTAATATGGTTGTCTTGGCGAGGATTCGCTTTAAAGTCTATACCAACCAATTTTTACTTGGAAAATCTAATTGCTCTTGACTTGCGGAACAGCAGCCTCCAACATGTTTGGAAGGGAACTAGGTTTCTTCCAAGACTGAAAATCTTAAATCTCAGTCATTCACATGGCCTTGTGACAACCCCTGACTTGTCAGGATCCCCTGACCTCGAGAGATTAATTCTTAAAGATTGCATAAATTTGAAAGAGGTGGATGAATCCATTGGAGATTTGGAGAAACTTGTTTTCTTGAATCTAAAAGATTGCAAAAATCTTATGAAGCTTCCAATAAGAATTAGTATGCTGCGATCTCTCCAGGAACTTATTCTCTCTGGTTGCTCAAATCTTGTGCTGCCTGCCAGTAAGATCGTTGAAAATCAGTCAGACTCTACACCTAGTGACATGAAGAAAGTCAGTCTGTTATTTGCAGTTAAATCGTGGCAATCAATCCGATCATGGGTATTGCCAAGAAAAAATCTCCAACTTACCAGTGCAAGTTTGCCACAATTTTTAAAAAGCTTAGATATGGCGTACTGCAATCTGTCAGAAATTCCCAATGATCTTCCTAGTAGCCTATCCTCATTGGAGCGTTTGAATCTAGATGGAAACCCATTTTTGAGCCTGCCAGTAAACTTGAATGGTCTTAGTAAGCTCCAGAGACTTTCGTTGGATATGTGCCCAAACCTCGAAATGATTCCCGAGCTCCCACCAAGTGTAGAGGCTTTGATCGCAACTAGATGTACTTCATTGAAAAGAGTACTACTAAACTTACCCGACATGTTACCAACAATTAGACTTGCGGTTATTGCATGCGAGAATGTAGTTGAAATTCAAAACGTGTTTAAAAAAAGACCGTTGAGAAGCGTCGACATAGAAATGATCAAAGATATCGGTCTGTTCAATTTGGAATCCATAGGAAGCACTGAAGTTGAAATGTTCGACTACTTGACACTTACAAGAAGGAAGGGTCCTCTCCAGGGACTCGATGAATGTGGTATATTTAGCATATTCCTTCCCGGGAGCGAGGTTCCAGATTGGTTCTGCTACAAGTCGAGCATGGGTAACTCTGAGCTGTCTATAACTATACCTCCACATCTTAATTTGAAGATCCGAGGCCTAAATGCATGTGTTGTGTATGCACATGATGAGGTTGATGATGATAAGGGAAGGGGATTTTACCGCGCTCCCTTTCTTAACATCAGTAATGAGACCAAGGGTCTTAAGTGGACCTATGTGCCAGTCACAATAGGGTTTTCAAAAGAGAAGGAACATATGTTATGGCTTAGTCATTGGCGATTCGCAAACGATGAATTGAAGGGTGGGGATGAAATACGTGTTTCGGTTAGAGTAGATAAAGATGATATCTTGATAAAGGAATTTGGAATCCAGCTTGTGTACGAGCAGAATAATAATGAGGGTACTGTAACTGAAGATATAACCACAATGTTCCAGCATGAAACAACTCCTCCTTCGACCCAGAATCATGTCGTTGCTGGAGATGTGTCAGCATCAGCATCAAGGTTCCATAAAAATAAAAATAATAAGGTGGGTGAATACTATATTTGTAATCATAATGGATGGTATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1176

Amino Acids

133.19

Weight (kDa)

7.03

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 717
AatII GACGTC 1 cut(s) 1744
AccB1I GGYRCC 1 cut(s) 267
AccI GTMKAC 1 cut(s) 2805
AccII CGCG 2 cut(s) 1524, 3120
AciI CCGC 3 cut(s) 1987, 2738, 3118
AcsI RAATTY 8 cut(s) 375, 734, 1425, 1900, 2137, 2487, 2766, 3314
AcuI CTGAAG 4 cut(s) 58, 1290, 2883, 3387
AcyI GRCGYC 1 cut(s) 1741
AdeI CACNNNGTG 2 cut(s) 2336, 2651
AfaI GTAC 9 cut(s) 500, 798, 1555, 1606, 2471, 2680, 2697, 3335, 3358
AfeI AGCGCT 1 cut(s) 410
AfiI CCNNNNNNNGG 8 cut(s) 45, 118, 264, 360, 1124, 1130, 2395, 2915
AflII CTTAAG 1 cut(s) 3158
AflIII ACRYGT 5 cut(s) 624, 2005, 2715, 2775, 3271
AgeI ACCGGT 1 cut(s) 45
AhdI GACNNNNNGTC 1 cut(s) 3153
AhlI ACTAGT 1 cut(s) 280
AjiI CACGTC 1 cut(s) 343
AjnI CCWGG 6 cut(s) 982, 1123, 1543, 1760, 2253, 2913
Alw21I GWGCWC 2 cut(s) 53, 2643
Alw26I GTCTC 4 cut(s) 78, 222, 2590, 3140
Alw44I GTGCAC 1 cut(s) 49
Ama87I CYCGRG 5 cut(s) 219, 259, 2108, 2636, 2954
Aor51HI AGCGCT 1 cut(s) 410
AoxI GGCC 4 cut(s) 616, 1621, 2068, 3052
ApaLI GTGCAC 1 cut(s) 49
ApeKI GCWGC 8 cut(s) 5, 19, 747, 1222, 1734, 1995, 2242, 2290
ApoI RAATTY 8 cut(s) 375, 734, 1425, 1900, 2137, 2487, 2766, 3314
AseI ATTAAT 1 cut(s) 2117
AsiGI ACCGGT 1 cut(s) 45
Asp700I GAANNNNTTC 5 cut(s) 975, 1287, 1726, 2262, 2631
AspLEI GCGC 2 cut(s) 411, 3122
AspS9I GGNCC 4 cut(s) 295, 1621, 2906, 3165
AsuC2I CCSGG 2 cut(s) 2955, 2956
AsuHPI GGTGA 3 cut(s) 35, 101, 3500
AvaI CYCGRG 5 cut(s) 219, 259, 2108, 2636, 2954
AvaII GGWCC 3 cut(s) 295, 2906, 3165
AxyI CCTNAGG 1 cut(s) 1536
BaeGI GKGCMC 3 cut(s) 53, 318, 2618
BamHI GGATCC 4 cut(s) 138, 346, 1540, 2096
BanI GGYRCC 1 cut(s) 267
BanII GRGCYC 1 cut(s) 2643
BbsI GAAGAC 3 cut(s) 229, 1372, 1653
Bbv12I GWGCWC 2 cut(s) 53, 2643
BbvI GCAGC 7 cut(s) 31, 759, 1209, 1746, 2007, 2229, 2277
BcgI CGANNNNNNTGC 2 cut(s) 2355, 2389
BciT130I CCWGG 6 cut(s) 984, 1125, 1545, 1762, 2255, 2915
BciVI GTATCC 1 cut(s) 235
BclI TGATCA 3 cut(s) 106, 288, 2817
BcnI CCSGG 2 cut(s) 2955, 2956
BcoDI GTCTC 4 cut(s) 78, 222, 2590, 3140
BcuI ACTAGT 1 cut(s) 280
BfaI CTAG 9 cut(s) 272, 281, 1008, 1557, 2022, 2334, 2505, 2537, 2673
BfmI CTRYAG 4 cut(s) 115, 663, 1155, 1223
BfoI RGCGCY 1 cut(s) 412
BfrI CTTAAG 1 cut(s) 3158
BfuI GTATCC 1 cut(s) 235
BglII AGATCT 1 cut(s) 823
BisI GCNGC 8 cut(s) 6, 20, 748, 1223, 1735, 1996, 2243, 2291
BlsI GCNGC 8 cut(s) 7, 21, 749, 1224, 1736, 1997, 2244, 2292
BmcAI AGTACT 1 cut(s) 2697
Bme1390I CCNGG 8 cut(s) 984, 1125, 1545, 1762, 2255, 2915, 2955, 2956
Bme18I GGWCC 3 cut(s) 295, 2906, 3165
BmeRI GACNNNNNGTC 1 cut(s) 3153
BmeT110I CYCGRG 5 cut(s) 219, 259, 2108, 2636, 2954
BmgBI CACGTC 1 cut(s) 343
BmgT120I GGNCC 4 cut(s) 295, 1621, 2906, 3165
BmrFI CCNGG 8 cut(s) 984, 1125, 1545, 1762, 2255, 2915, 2955, 2956
BmsI GCATC 4 cut(s) 49, 1564, 3455, 3461
BoxI GACNNNNGTC 2 cut(s) 1329, 1446
BpiI GAAGAC 3 cut(s) 229, 1372, 1653
BplI GAGNNNNNCTC 2 cut(s) 436, 468
BpmI CTGGAG 4 cut(s) 2237, 2576, 2897, 3453
BpuEI CTTGAG 3 cut(s) 88, 1091, 1106
BpuMI CCSGG 2 cut(s) 2955, 2956
BsaAI YACGTR 1 cut(s) 3272
BsaHI GRCGYC 1 cut(s) 1741
BsaI GGTCTC 1 cut(s) 3140
BsaJI CCNNGG 5 cut(s) 243, 2914, 2954, 3049, 3150
BsaWI WCCGGW 1 cut(s) 45
BsaXI ACNNNNNCTCC 8 cut(s) 468, 498, 648, 678, 3010, 3040, 3388, 3418
Bsc4I CCNNNNNNNGG 8 cut(s) 45, 118, 264, 360, 1124, 1130, 2395, 2915
Bse118I RCCGGY 1 cut(s) 45
Bse1I ACTGG 7 cut(s) 552, 1606, 1677, 2298, 2427, 2564, 3176
Bse21I CCTNAGG 1 cut(s) 1536
Bse3DI GCAATG 2 cut(s) 699, 1053
BseBI CCWGG 6 cut(s) 984, 1125, 1545, 1762, 2255, 2915
BseDI CCNNGG 5 cut(s) 243, 2914, 2954, 3049, 3150
BseLI CCNNNNNNNGG 8 cut(s) 45, 118, 264, 360, 1124, 1130, 2395, 2915
BseMI GCAATG 2 cut(s) 699, 1053
BseMII CTCAG 5 cut(s) 469, 1306, 1527, 2068, 2997
BseNI ACTGG 7 cut(s) 552, 1606, 1677, 2298, 2427, 2564, 3176
BseRI GAGGAG 2 cut(s) 189, 3393
BseSI GKGCMC 3 cut(s) 53, 318, 2618
BseXI GCAGC 7 cut(s) 31, 759, 1209, 1746, 2007, 2229, 2277
Bsh1236I CGCG 2 cut(s) 1524, 3120
BshFI GGCC 4 cut(s) 618, 1623, 2070, 3054
BshNI GGYRCC 1 cut(s) 267
BshTI ACCGGT 1 cut(s) 45
BsiHKAI GWGCWC 2 cut(s) 53, 2643
BsiHKCI CYCGRG 5 cut(s) 219, 259, 2108, 2636, 2954
BsiSI CCGG 2 cut(s) 46, 2955
BslFI GGGAC 1 cut(s) 2931
BslI CCNNNNNNNGG 8 cut(s) 45, 118, 264, 360, 1124, 1130, 2395, 2915
BsmAI GTCTC 4 cut(s) 78, 222, 2590, 3140
BsmFI GGGAC 1 cut(s) 2931
BsmI GAATGC 3 cut(s) 31, 62, 969
BsnI GGCC 4 cut(s) 618, 1623, 2070, 3054
Bso31I GGTCTC 1 cut(s) 3140
BsoBI CYCGRG 5 cut(s) 219, 259, 2108, 2636, 2954
Bsp1286I GDGCHC 4 cut(s) 53, 318, 2618, 2643
Bsp1407I TGTACA 1 cut(s) 796
Bsp68I TCGCGA 1 cut(s) 1524
BspACI CCGC 3 cut(s) 1987, 2738, 3118
BspANI GGCC 4 cut(s) 618, 1623, 2070, 3054
BspCNI CTCAG 5 cut(s) 468, 1305, 1528, 2067, 2998
BspFNI CGCG 2 cut(s) 1524, 3120
BspHI TCATGA 2 cut(s) 1033, 1321
BspMAI CTGCAG 1 cut(s) 1227
BspT107I GGYRCC 1 cut(s) 267
BspTI CTTAAG 1 cut(s) 3158
BspTNI GGTCTC 1 cut(s) 3140
BsrDI GCAATG 2 cut(s) 699, 1053
BsrFI RCCGGY 1 cut(s) 45
BsrGI TGTACA 1 cut(s) 796
BsrI ACTGG 7 cut(s) 552, 1606, 1677, 2298, 2427, 2564, 3176
BssAI RCCGGY 1 cut(s) 45
BssECI CCNNGG 5 cut(s) 243, 2914, 2954, 3049, 3150
BssNI GRCGYC 1 cut(s) 1741
BssT1I CCWWGG 2 cut(s) 243, 3150
Bst2UI CCWGG 6 cut(s) 984, 1125, 1545, 1762, 2255, 2915
Bst4CI ACNGT 6 cut(s) 299, 1169, 1390, 1727, 2793, 3361
BstACI GRCGYC 1 cut(s) 1741
BstAFI CTTAAG 1 cut(s) 3158
BstAUI TGTACA 1 cut(s) 796
BstBAI YACGTR 1 cut(s) 3272
BstC8I GCNNGC 6 cut(s) 17, 1104, 1832, 2295, 2561, 2749
BstENI CCTNNNNNAGG 1 cut(s) 1128
BstFNI CGCG 2 cut(s) 1524, 3120
BstH2I RGCGCY 1 cut(s) 412
BstHHI GCGC 2 cut(s) 411, 3122
BstMAI GTCTC 4 cut(s) 78, 222, 2590, 3140
BstMWI GCNNNNNNNGC 5 cut(s) 78, 710, 973, 2666, 2744
BstNI CCWGG 6 cut(s) 984, 1125, 1545, 1762, 2255, 2915
BstNSI RCATGY 7 cut(s) 628, 1106, 1834, 2009, 2719, 2751, 3066
BstPAI GACNNNNGTC 2 cut(s) 1329, 1446
BstSCI CCNGG 8 cut(s) 982, 1123, 1543, 1760, 2253, 2913, 2953, 2954
BstSFI CTRYAG 4 cut(s) 115, 663, 1155, 1223
BstSLI GKGCMC 3 cut(s) 53, 318, 2618
BstUI CGCG 2 cut(s) 1524, 3120
BstV1I GCAGC 7 cut(s) 31, 759, 1209, 1746, 2007, 2229, 2277
BstV2I GAAGAC 3 cut(s) 229, 1372, 1653
BstX2I RGATCY 8 cut(s) 138, 346, 823, 1141, 1202, 1540, 2096, 3045
BstYI RGATCY 8 cut(s) 138, 346, 823, 1141, 1202, 1540, 2096, 3045
Bsu36I CCTNAGG 1 cut(s) 1536
BsuI GTATCC 1 cut(s) 235
BsuRI GGCC 4 cut(s) 618, 1623, 2070, 3054
BtrI CACGTC 1 cut(s) 343
BtsIMutI CAGTG 5 cut(s) 1165, 1684, 1879, 2434, 2859
BtuMI TCGCGA 1 cut(s) 1524
Cac8I GCNNGC 6 cut(s) 17, 1104, 1832, 2295, 2561, 2749
CciI TCATGA 2 cut(s) 1033, 1321
CfoI GCGC 2 cut(s) 411, 3122
Cfr10I RCCGGY 1 cut(s) 45
Cfr13I GGNCC 4 cut(s) 295, 1621, 2906, 3165
Cfr9I CCCGGG 1 cut(s) 2954
CseI GACGC 1 cut(s) 2791
Csp6I GTAC 9 cut(s) 499, 797, 1554, 1605, 2470, 2679, 2696, 3334, 3357
CspAI ACCGGT 1 cut(s) 45
CspCI CAANNNNNGTGG 2 cut(s) 1402, 1437
CviQI GTAC 9 cut(s) 499, 797, 1554, 1605, 2470, 2679, 2696, 3334, 3357
DraI TTTAAA 3 cut(s) 1935, 2451, 2782
DraIII CACNNNGTG 2 cut(s) 2336, 2651
DriI GACNNNNNGTC 1 cut(s) 3153
Eam1105I GACNNNNNGTC 1 cut(s) 3153
Ecl136II GAGCTC 1 cut(s) 2641
Eco130I CCWWGG 2 cut(s) 243, 3150
Eco147I AGGCCT 2 cut(s) 618, 3054
Eco24I GRGCYC 1 cut(s) 2643
Eco31I GGTCTC 1 cut(s) 3140
Eco32I GATATC 2 cut(s) 2827, 3301
Eco47I GGWCC 3 cut(s) 295, 2906, 3165
Eco47III AGCGCT 1 cut(s) 410
Eco53kI GAGCTC 1 cut(s) 2641
Eco57I CTGAAG 4 cut(s) 58, 1290, 2883, 3387
Eco81I CCTNAGG 1 cut(s) 1536
Eco88I CYCGRG 5 cut(s) 219, 259, 2108, 2636, 2954
EcoICRI GAGCTC 1 cut(s) 2641
EcoNI CCTNNNNNAGG 1 cut(s) 1128
EcoO109I RGGNCCY 2 cut(s) 1621, 2906
EcoRI GAATTC 1 cut(s) 1425
EcoRII CCWGG 6 cut(s) 982, 1123, 1543, 1760, 2253, 2913
EcoRV GATATC 2 cut(s) 2827, 3301
EcoT14I CCWWGG 2 cut(s) 243, 3150
EcoT22I ATGCAT 3 cut(s) 64, 1246, 3064
EcoT38I GRGCYC 1 cut(s) 2643
ErhI CCWWGG 2 cut(s) 243, 3150
FalI AAGNNNNNCTT 2 cut(s) 1053, 1085
FaqI GGGAC 1 cut(s) 2931
FauNDI CATATG 1 cut(s) 3210
FbaI TGATCA 3 cut(s) 106, 288, 2817
FblI GTMKAC 1 cut(s) 2805
Fnu4HI GCNGC 8 cut(s) 6, 20, 748, 1223, 1735, 1996, 2243, 2291
FokI GGATG 9 cut(s) 337, 493, 688, 875, 966, 1107, 1271, 2165, 3275
FriOI GRGCYC 1 cut(s) 2643
Fsp4HI GCNGC 8 cut(s) 6, 20, 748, 1223, 1735, 1996, 2243, 2291
FspBI CTAG 9 cut(s) 272, 281, 1008, 1557, 2022, 2334, 2505, 2537, 2673
GlaI GCGC 2 cut(s) 410, 3121
GluI GCNGC 8 cut(s) 6, 20, 748, 1223, 1735, 1996, 2243, 2291
GsuI CTGGAG 4 cut(s) 2237, 2576, 2897, 3453
HaeII RGCGCY 1 cut(s) 412
HaeIII GGCC 4 cut(s) 618, 1623, 2070, 3054
HapII CCGG 2 cut(s) 46, 2955
HgaI GACGC 1 cut(s) 2791
HhaI GCGC 2 cut(s) 411, 3122
Hin1I GRCGYC 1 cut(s) 1741
Hin6I GCGC 2 cut(s) 409, 3120
HinP1I GCGC 2 cut(s) 409, 3120
HincII GTYRAC 3 cut(s) 1441, 1479, 2806
HindII GTYRAC 3 cut(s) 1441, 1479, 2806
HindIII AAGCTT 3 cut(s) 1300, 2219, 2455
HpaI GTTAAC 1 cut(s) 1479
HpaII CCGG 2 cut(s) 46, 2955
HphI GGTGA 3 cut(s) 35, 101, 3500
Hpy166II GTNNAC 9 cut(s) 51, 799, 931, 1441, 1479, 2569, 2806, 3165, 3334
Hpy188I TCNGA 8 cut(s) 458, 823, 1295, 2323, 2390, 2485, 3007, 3050
Hpy8I GTNNAC 9 cut(s) 51, 799, 931, 1441, 1479, 2569, 2806, 3165, 3334
Hpy99I CGWCG 3 cut(s) 1743, 1746, 2807
HpyCH4III ACNGT 6 cut(s) 299, 1169, 1390, 1727, 2793, 3361
HpyCH4IV ACGT 7 cut(s) 342, 895, 1330, 1552, 1741, 2775, 3271
HpyF10VI GCNNNNNNNGC 5 cut(s) 78, 710, 973, 2666, 2744
HpySE526I ACGT 7 cut(s) 342, 895, 1330, 1552, 1741, 2775, 3271
Hsp92I GRCGYC 1 cut(s) 1741
HspAI GCGC 2 cut(s) 409, 3120
Ksp22I TGATCA 3 cut(s) 106, 288, 2817
KspAI GTTAAC 1 cut(s) 1479
LmnI GCTCC 6 cut(s) 176, 2524, 2595, 2646, 2958, 3127
Lsp1109I GCAGC 7 cut(s) 31, 759, 1209, 1746, 2007, 2229, 2277
LweI GCATC 4 cut(s) 49, 1564, 3455, 3461
MaeI CTAG 9 cut(s) 272, 281, 1008, 1557, 2022, 2334, 2505, 2537, 2673
MaeII ACGT 7 cut(s) 342, 895, 1330, 1552, 1741, 2775, 3271
MaeIII GTNAC 8 cut(s) 1133, 1685, 2074, 2336, 2718, 2999, 3178, 3361
MflI RGATCY 8 cut(s) 138, 346, 823, 1141, 1202, 1540, 2096, 3045
MhlI GDGCHC 4 cut(s) 53, 318, 2618, 2643
MlyI GAGTC 6 cut(s) 224, 512, 585, 1667, 2318, 2913
MmeI TCCRAC 5 cut(s) 574, 634, 2026, 2443, 2586
Mph1103I ATGCAT 3 cut(s) 64, 1246, 3064
MroXI GAANNNNTTC 5 cut(s) 975, 1287, 1726, 2262, 2631
MslI CAYNNNNRTG 4 cut(s) 323, 1640, 2064, 2813
MspCI CTTAAG 1 cut(s) 3158
MspI CCGG 2 cut(s) 46, 2955
MspR9I CCNGG 8 cut(s) 984, 1125, 1545, 1762, 2255, 2915, 2955, 2956
Mva1269I GAATGC 3 cut(s) 31, 62, 969
MvaI CCWGG 6 cut(s) 984, 1125, 1545, 1762, 2255, 2915
MvnI CGCG 2 cut(s) 1524, 3120
MwoI GCNNNNNNNGC 5 cut(s) 78, 710, 973, 2666, 2744
NciI CCSGG 2 cut(s) 2955, 2956
NdeI CATATG 1 cut(s) 3210
NmuCI GTSAC 3 cut(s) 2074, 2336, 3178
NruI TCGCGA 1 cut(s) 1524
NsiI ATGCAT 3 cut(s) 64, 1246, 3064
NspI RCATGY 7 cut(s) 628, 1106, 1834, 2009, 2719, 2751, 3066
PaeI GCATGC 3 cut(s) 1106, 1834, 2751
PaeR7I CTCGAG 3 cut(s) 219, 259, 2108
PagI TCATGA 2 cut(s) 1033, 1321
PceI AGGCCT 2 cut(s) 618, 3054
PciI ACATGT 3 cut(s) 624, 2005, 2715
PcsI WCGNNNNNNNCGW 1 cut(s) 892
PctI GAATGC 3 cut(s) 31, 62, 969
PdmI GAANNNNTTC 5 cut(s) 975, 1287, 1726, 2262, 2631
PfoI TCCNGGA 3 cut(s) 982, 1543, 2253
PinAI ACCGGT 1 cut(s) 45
PkrI GCNGC 8 cut(s) 7, 21, 749, 1224, 1736, 1997, 2244, 2292
PleI GAGTC 6 cut(s) 223, 511, 584, 1666, 2318, 2913
PpsI GAGTC 6 cut(s) 223, 511, 584, 1666, 2318, 2913
Ppu21I YACGTR 1 cut(s) 3272
PpuMI RGGWCCY 1 cut(s) 2906
PscI ACATGT 3 cut(s) 624, 2005, 2715
PshAI GACNNNNGTC 2 cut(s) 1329, 1446
PshBI ATTAAT 1 cut(s) 2117
PsiI TTATAA 1 cut(s) 717
Psp124BI GAGCTC 1 cut(s) 2643
Psp5II RGGWCCY 1 cut(s) 2906
Psp6I CCWGG 6 cut(s) 982, 1123, 1543, 1760, 2253, 2913
PspGI CCWGG 6 cut(s) 982, 1123, 1543, 1760, 2253, 2913
PspPI GGNCC 4 cut(s) 295, 1621, 2906, 3165
PspPPI RGGWCCY 1 cut(s) 2906
PstI CTGCAG 1 cut(s) 1227
PsuI RGATCY 8 cut(s) 138, 346, 823, 1141, 1202, 1540, 2096, 3045
RruI TCGCGA 1 cut(s) 1524
RsaI GTAC 9 cut(s) 500, 798, 1555, 1606, 2471, 2680, 2697, 3335, 3358
RsaNI GTAC 9 cut(s) 499, 797, 1554, 1605, 2470, 2679, 2696, 3334, 3357
RseI CAYNNNNRTG 4 cut(s) 323, 1640, 2064, 2813
SacI GAGCTC 1 cut(s) 2643
SalI GTCGAC 1 cut(s) 2804
SatI GCNGC 8 cut(s) 6, 20, 748, 1223, 1735, 1996, 2243, 2291
Sau96I GGNCC 4 cut(s) 295, 1621, 2906, 3165
ScaI AGTACT 1 cut(s) 2697
SchI GAGTC 6 cut(s) 224, 512, 585, 1667, 2318, 2913
ScrFI CCNGG 8 cut(s) 984, 1125, 1545, 1762, 2255, 2915, 2955, 2956
SduI GDGCHC 4 cut(s) 53, 318, 2618, 2643
SfaNI GCATC 4 cut(s) 49, 1564, 3455, 3461
SfcI CTRYAG 4 cut(s) 115, 663, 1155, 1223
Sfr274I CTCGAG 3 cut(s) 219, 259, 2108
SgrAI CRCCGGYG 1 cut(s) 45
SinI GGWCC 3 cut(s) 295, 2906, 3165
SlaI CTCGAG 3 cut(s) 219, 259, 2108
SmaI CCCGGG 1 cut(s) 2956
SmiMI CAYNNNNRTG 4 cut(s) 323, 1640, 2064, 2813
SmlI CTYRAG 7 cut(s) 67, 219, 259, 1070, 1085, 2108, 3158
SmoI CTYRAG 7 cut(s) 67, 219, 259, 1070, 1085, 2108, 3158
SpeI ACTAGT 1 cut(s) 280
SphI GCATGC 3 cut(s) 1106, 1834, 2751
SseBI AGGCCT 2 cut(s) 618, 3054
SsiI CCGC 3 cut(s) 1987, 2738, 3118
SspI AATATT 1 cut(s) 184
SspMI CTAG 9 cut(s) 272, 281, 1008, 1557, 2022, 2334, 2505, 2537, 2673
SstI GAGCTC 1 cut(s) 2643
StuI AGGCCT 2 cut(s) 618, 3054
StyD4I CCNGG 8 cut(s) 982, 1123, 1543, 1760, 2253, 2913, 2953, 2954
StyI CCWWGG 2 cut(s) 243, 3150
TaaI ACNGT 6 cut(s) 299, 1169, 1390, 1727, 2793, 3361
TaiI ACGT 7 cut(s) 345, 898, 1333, 1555, 1744, 2778, 3274
TaqII GACCGA 1 cut(s) 2819
TatI WGTACW 3 cut(s) 796, 2678, 2695
TscAI CASTG 5 cut(s) 1172, 1684, 1879, 2434, 2866
TseFI GTSAC 3 cut(s) 2074, 2336, 3178
TseI GCWGC 8 cut(s) 5, 19, 747, 1222, 1734, 1995, 2242, 2290
Tsp45I GTSAC 3 cut(s) 2074, 2336, 3178
TspGWI ACGGA 1 cut(s) 24
TspMI CCCGGG 1 cut(s) 2954
TspRI CASTG 5 cut(s) 1172, 1684, 1879, 2434, 2866
Vha464I CTTAAG 1 cut(s) 3158
VneI GTGCAC 1 cut(s) 49
VpaK11BI GGWCC 3 cut(s) 295, 2906, 3165
VspI ATTAAT 1 cut(s) 2117
XagI CCTNNNNNAGG 1 cut(s) 1128
XapI RAATTY 8 cut(s) 375, 734, 1425, 1900, 2137, 2487, 2766, 3314
XbaI TCTAGA 1 cut(s) 2536
XceI RCATGY 7 cut(s) 628, 1106, 1834, 2009, 2719, 2751, 3066
XcmI CCANNNNNNNNNTGG 2 cut(s) 1957, 2167
XhoI CTCGAG 3 cut(s) 219, 259, 2108
XmaI CCCGGG 1 cut(s) 2954
XmiI GTMKAC 1 cut(s) 2805
XmnI GAANNNNTTC 5 cut(s) 975, 1287, 1726, 2262, 2631
XspI CTAG 9 cut(s) 272, 281, 1008, 1557, 2022, 2334, 2505, 2537, 2673
ZraI GACGTC 1 cut(s) 1742
ZrmI AGTACT 1 cut(s) 2697
Zsp2I ATGCAT 3 cut(s) 64, 1246, 3064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.