Rorug04G0057900

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000654
Physical Location & Seq
Reverse (-)
9075826 .. 9082427
6602 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0057900.1

Sequence Viewer

Length: 3057 bp
ATGGTGTCACTGGACCCAGATGACTTCTTGGCCGGCCTTGCCGATGACCCGGACTATCCCAGCCGTCCATTTCCGGCAACCGAGAACCTAGCTGAGGAAGAAGAGCTGGAATGGCTATCCAACAAGGACCTGTTCCCGGCAGTCGAAACCTGCCTGCTGACGGAGGAGCCACCAAAAAAGGCTATGGCCAATGCCGAGCAGGGGAAGCCGGTCTCTGTCTCGGTGGTTAAAGCAAGCATTGGAGGATGTGGAGCAGTACTATTGCCGCCAATGCCGAGCAGGAAAACAAAGGATGAGAGTGCCAAAGTGAAGAGTATGAGAGTTTGCGCATATTGCGGGGTTACACAGACCCCACAGTGGCCGGAGGGTCCCTTGGGGATATGTAATGCCTGCGGTTTTAGCTACAAGTCCGGGCGGCTTTGCACTGAAGACAGTCCCAAAGTGCAGAGCATTAAGAGTGACAAGGTGAATGGAATTAAGAGTGACAAGGTGAAGACCATTAAGAGAAAGTGCACACATTGTGGGATTACACAGACTCCGCAGTGGCGGTTGGGTCCCCTTGGACCGCAGACCCTGTGTAATGCTTGTGGGGTTAGGTACAAGTCTGGGAGGCTTTGCCCCGAATACCGCCCTGCAAATAGCCCTACGTTCTCGCAGGAGTTGCATTCGAATAAGCACAGGAAGGTAATGGAGATCAGGAAGCAAATCTATGGGAATGAGGGGACGGTGGTGAGACCTGTGGATAAATGCACAAACAGTGCCGCCGCCACTGCCCTAGAGAAGATGAAGAAAGACATGTTCCCGACTGTCACAACAGTGGAGCAACCAAGAAGAAGTGTCATTGCTGAGCAGCAGAGCCCTGTTTCGGTGCTTGAAAACAGCACAAACAGCAGCACCACCCTCAAGCCCCACCAACCCCCAAGCAAATTTCTGAGGGAGCAGCAGCTGTTCTGTAACCAACCTAACAATAAGCACAACCAGGAAGACACTGCCAAAGTGGAAATTAAGAGAAACATCATCCTCATGAGTTCCTGTGGGACCCTTGAGCTGCCTTATAAAGCGCCAAGCGAAGTTTTGGGGCAGCAGCAGCTGTTTAGTTGCCAACCTACCAGCAAACGCAGAAAGAAAAACACTGTTGAAATGAAGAATGAGGGAAACCACGCCACCCTCACGAGTTCCTGTGGGAACCTTGAGCCGCCTCACCAAGCGCCAAGTCAATTTCTGGAGCAGCACCAGCTGTTTGATGACCAACCTAACAACAAGTGCAACAAGAAACACACTACCAAAGTGGAGATGACCATGAAACCTGTGGATATATGCAACACCACCCTCATGAAGTCTAAGAAAGACTCATTACCGGCTGTCGAAACATTGAATATCTTGGAGCATTCAAGAGGCATTTTTATTGCTGAGCAGCAGAGCCCTGTAGTGGGGATTGCGGTAAATTGCCAGAATTGTGGTGCTGAACAGAGCCCGCAATGGTGGGAGGGTCCCCTTGGACCCAAAACCATATGTCTTTCCTGTGGGCTGGCCAAGTACACAGGCAGGCGGAAAAGAAGTATGTCTTTAGTTTCTAACAAGAAGCGCAAAAAGAAAGACACTGCGAAAGTGGAGATGGCAGTGAAACCTGGTGATAAATGCACAAACAGCACCACTGCCCTCATGAACTCCAAGAAAGACCCCTCACCGGCTGTCGGTACATCGAATATCTCAGAGCAACCAAGCGTCATTGTCATTGCCGAGCAGCAGAGCCCTGCATCTGTGGTTGAAAACAGCATAAGCAGCAGCATGATGCTCATGAGCTCCTCTGGGACTGTCAACCTGCACAACCAAGCCCCAAGTGAATTTCCGCAGCAGAACCAGTTTTGTGACCAAGCTAACAACAAGCCCAAAGAGAAAGACACTACCACAGTGGAGTTTGAGGGAAACAGCTCTACCCTCATGAGGTCCTCTGGGGCCCTTGAGCCACTCCACCAAGCTCCAAACGAATTTCTGGACCAGCAGCAGCAAGCTAAGAACAAGCCCAACAAGAAAGACACTGCTAAAGTGGAGAGTGAGGGAAACAGCACCACCCTCATGAGCTCTTGTGGGACTAACAAACCGCCCGACCAAGCACAGAGCGAATTTCTGGGGCAACAGCAGCTGTTTTGTAACCAAGCTAACAATGGGACCAAAAAGGAAAACACTGCCAAAGGGGAGAGTGAGGGGAAGTGCCAGAATTGTGGGGCTGCGCAAACCCCGAGTTGGTTGGGAGGTCCCCTTGGACCAAAAACCCTATGTCATGCCTGTGGGCTGGCTAAGTGCACGGCCGGCCGGCAAAGAAGTATTGCTGTGGAGGTTGGAAAACTGCCCTGTCCATCACCTGCTACGGGAGAGTTTACTAATTCGACTTGCACTTTTCCGGTTCTTGAAGAGTTCCATCTCTCGCAGAAACCAAGCGACATAGCCAATGCCGAGCAACAGAAACCTTTTTCAGTTCTTGAAAGCAGCACAAACAACAGCATAGCCCTCATGAGCTCCTGCAGGATAAAAATCCCCCACCGAGCCCGAAGCAAGGTCCTCCGTAGAAGAAGAAGCACAATTCCAGGGCAGCAAGTTGGGATTGGAAAAAACTGTATGCAATGCGGAAAACAGACAAATAGATGGCGGATGGGTCCCCTCGGACCCACAACTCTATGTAATGCCTGTGGGAATAAGTATCAGCCCTTGTGGGGAAGAAGCGGCGTAACAGTGGACATTGCCAAAAACTATCATCATTGCGGGTCTGAGGAGACCACGCAATTGCTATTGTGTCCCCTTGGACCCAAAACCCTATGTACACTCTGTTATCATTGGTTTAAAGCCAGTGCTGAAGTGGAGATTGGGAGAAAATGCCAGCATTGTGGGGCTGAGAAAACCCCGCTTTGGCGGTCAGGTCCCCTGGGTTCCAAAACGCTGTGTAATGCATGTGGGCTTAGGTGGTACAAATTCGGGGATCTTTGCCGAGACCGCTCTGCAAGTAGCCCAACTCTCTTGAGGGAGTTGCATTCAAATTGTTACAGGAATGTGCTGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1018

Amino Acids

111.63

Weight (kDa)

9.03

Isoelectric Point (pI)

60.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATA PF00320 109 - 140 1.7e-06 GATA zinc finger
GATA PF00320 171 - 205 3.5e-13 GATA zinc finger
GATA PF00320 483 - 511 4.8e-07 GATA zinc finger
GATA PF00320 738 - 766 8.2e-07 GATA zinc finger
GATA PF00320 958 - 989 5e-14 GATA zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1056
AarI CACCTGC 1 cut(s) 2371
Acc16I TGCGCA 2 cut(s) 328, 2232
Acc36I ACCTGC 3 cut(s) 158, 1830, 2371
AccBSI CCGCTC 1 cut(s) 2991
AclWI GGATC 1 cut(s) 2982
AcoI YGGCCR 6 cut(s) 30, 186, 359, 1530, 2307, 2311
AcsI RAATTY 5 cut(s) 926, 1844, 1988, 2123, 2966
AcuI CTGAAG 2 cut(s) 447, 2871
AdeI CACNNNGTG 1 cut(s) 521
AfaI GTAC 6 cut(s) 258, 599, 1538, 1699, 2818, 2963
AflIII ACRYGT 1 cut(s) 795
AgsI TTSAA 8 cut(s) 875, 1139, 1375, 1392, 1769, 2411, 2483, 3030
AjnI CCWGG 4 cut(s) 978, 1627, 2584, 2919
AjuI GAANNNNNNNTTGG 2 cut(s) 2844, 2876
AleI CACNNNNGTG 1 cut(s) 815
Alw21I GWGCWC 5 cut(s) 515, 1805, 2084, 2306, 2519
Alw26I GTCTC 5 cut(s) 217, 223, 727, 2765, 2979
Alw44I GTGCAC 2 cut(s) 511, 2302
AlwI GGATC 1 cut(s) 2982
AlwNI CAGNNNCTG 4 cut(s) 574, 946, 1090, 2143
Ama87I CYCGRG 1 cut(s) 2239
AoxI GGCC 8 cut(s) 30, 34, 186, 359, 1530, 1956, 2307, 2311
ApaI GGGCCC 1 cut(s) 1960
ApaLI GTGCAC 2 cut(s) 511, 2302
ApoI RAATTY 5 cut(s) 926, 1844, 1988, 2123, 2966
AspLEI GCGC 5 cut(s) 329, 1063, 1210, 1587, 2233
AsuC2I CCSGG 3 cut(s) 50, 137, 412
AsuHPI GGTGA 7 cut(s) 478, 502, 742, 1193, 1643, 1677, 2352
AsuII TTCGAA 1 cut(s) 668
AvaI CYCGRG 1 cut(s) 2239
BaeGI GKGCMC 3 cut(s) 515, 1960, 2306
BalI TGGCCA 2 cut(s) 188, 1532
BanII GRGCYC 9 cut(s) 860, 1424, 1475, 1754, 1805, 1960, 2084, 2519, 2548
BauI CACGAG 1 cut(s) 1171
BbsI GAAGAC 3 cut(s) 435, 500, 990
Bbv12I GWGCWC 5 cut(s) 515, 1805, 2084, 2306, 2519
BbvCI CCTCAGC 1 cut(s) 93
BccI CCATC 5 cut(s) 1609, 2365, 2427, 2637, 2644
BceAI ACGGC 2 cut(s) 48, 2322
BciT130I CCWGG 4 cut(s) 980, 1629, 2586, 2921
BcnI CCSGG 3 cut(s) 50, 137, 412
BcoDI GTCTC 5 cut(s) 217, 223, 727, 2765, 2979
BfaI CTAG 2 cut(s) 89, 776
BfmI CTRYAG 2 cut(s) 1425, 2521
BfoI RGCGCY 2 cut(s) 1064, 1211
BfuAI ACCTGC 3 cut(s) 158, 1830, 2371
BlpI GCTNAGC 2 cut(s) 846, 1410
BmcAI AGTACT 1 cut(s) 258
Bme1390I CCNGG 7 cut(s) 50, 137, 412, 980, 1629, 2586, 2921
BmeT110I CYCGRG 1 cut(s) 2239
BmrFI CCNGG 7 cut(s) 50, 137, 412, 980, 1629, 2586, 2921
BmsI GCATC 2 cut(s) 1766, 1782
BpiI GAAGAC 3 cut(s) 435, 500, 990
BpmI CTGGAG 1 cut(s) 1244
Bpu10I CCTNAGC 2 cut(s) 93, 2954
Bpu1102I GCTNAGC 2 cut(s) 846, 1410
Bpu14I TTCGAA 1 cut(s) 668
BpuEI CTTGAG 5 cut(s) 887, 1064, 1211, 1982, 3034
BpuMI CCSGG 3 cut(s) 50, 137, 412
BsaBI GATNNNNATC 1 cut(s) 2531
BsaI GGTCTC 4 cut(s) 217, 727, 2765, 2979
BsaJI CCNNGG 9 cut(s) 372, 559, 1495, 2260, 2585, 2659, 2797, 2919, 2920
BsaWI WCCGGW 1 cut(s) 2401
BsaXI ACNNNNNCTCC 2 cut(s) 520, 550
Bse118I RCCGGY 6 cut(s) 32, 208, 1357, 1687, 2309, 2313
Bse1I ACTGG 3 cut(s) 15, 1861, 2844
Bse3DI GCAATG 6 cut(s) 840, 1484, 1734, 2627, 2736, 2755
Bse8I GATNNNNATC 1 cut(s) 2531
BseBI CCWGG 4 cut(s) 980, 1629, 2586, 2921
BseDI CCNNGG 9 cut(s) 372, 559, 1495, 2260, 2585, 2659, 2797, 2919, 2920
BseGI GGATG 4 cut(s) 251, 298, 1017, 2655
BseJI GATNNNNATC 1 cut(s) 2531
BseMI GCAATG 6 cut(s) 840, 1484, 1734, 2627, 2736, 2755
BseMII CTCAG 7 cut(s) 84, 837, 923, 1401, 1725, 2757, 2880
BseNI ACTGG 3 cut(s) 15, 1861, 2844
BseRI GAGGAG 3 cut(s) 179, 1795, 2783
BseSI GKGCMC 3 cut(s) 515, 1960, 2306
BseX3I CGGCCG 2 cut(s) 2307, 2311
BseYI CCCAGC 1 cut(s) 59
BsgI GTGCAG 2 cut(s) 464, 1808
Bsh1285I CGRYCG 2 cut(s) 2310, 2314
BshFI GGCC 8 cut(s) 32, 36, 188, 361, 1532, 1958, 2309, 2313
BsiEI CGRYCG 2 cut(s) 2310, 2314
BsiHKAI GWGCWC 5 cut(s) 515, 1805, 2084, 2306, 2519
BsiHKCI CYCGRG 1 cut(s) 2239
BsmAI GTCTC 5 cut(s) 217, 223, 727, 2765, 2979
BsmI GAATGC 3 cut(s) 664, 1387, 3025
BsnI GGCC 8 cut(s) 32, 36, 188, 361, 1532, 1958, 2309, 2313
Bso31I GGTCTC 4 cut(s) 217, 727, 2765, 2979
BsoBI CYCGRG 1 cut(s) 2239
Bsp119I TTCGAA 1 cut(s) 668
Bsp120I GGGCCC 1 cut(s) 1956
Bsp1407I TGTACA 1 cut(s) 2816
Bsp143I GATC 2 cut(s) 693, 2974
Bsp1720I GCTNAGC 2 cut(s) 846, 1410
BspANI GGCC 8 cut(s) 32, 36, 188, 361, 1532, 1958, 2309, 2313
BspCNI CTCAG 7 cut(s) 85, 838, 924, 1402, 1724, 2758, 2881
BspHI TCATGA 7 cut(s) 1023, 1332, 1662, 1797, 1941, 2076, 2511
BspMAI CTGCAG 1 cut(s) 2525
BspMI ACCTGC 3 cut(s) 158, 1830, 2371
BspPI GGATC 1 cut(s) 2982
BspQI GCTCTTC 1 cut(s) 96
BspT104I TTCGAA 1 cut(s) 668
BspTNI GGTCTC 4 cut(s) 217, 727, 2765, 2979
BsrBI CCGCTC 1 cut(s) 2991
BsrDI GCAATG 6 cut(s) 840, 1484, 1734, 2627, 2736, 2755
BsrFI RCCGGY 6 cut(s) 32, 208, 1357, 1687, 2309, 2313
BsrGI TGTACA 1 cut(s) 2816
BsrI ACTGG 3 cut(s) 15, 1861, 2844
BssAI RCCGGY 6 cut(s) 32, 208, 1357, 1687, 2309, 2313
BssECI CCNNGG 9 cut(s) 372, 559, 1495, 2260, 2585, 2659, 2797, 2919, 2920
BssMI GATC 2 cut(s) 693, 2974
BssSI CACGAG 1 cut(s) 1171
BssT1I CCWWGG 5 cut(s) 372, 559, 1495, 2260, 2797
Bst2BI CACGAG 1 cut(s) 1171
Bst2UI CCWGG 4 cut(s) 980, 1629, 2586, 2921
Bst6I CTCTTC 3 cut(s) 96, 305, 2406
BstAPI GCANNNNNTGC 1 cut(s) 661
BstAUI TGTACA 1 cut(s) 2816
BstBI TTCGAA 1 cut(s) 668
BstENI CCTNNNNNAGG 1 cut(s) 92
BstF5I GGATG 4 cut(s) 251, 298, 1017, 2655
BstH2I RGCGCY 2 cut(s) 1064, 1211
BstHHI GCGC 5 cut(s) 329, 1063, 1210, 1587, 2233
BstKTI GATC 2 cut(s) 696, 2977
BstMAI GTCTC 5 cut(s) 217, 223, 727, 2765, 2979
BstMBI GATC 2 cut(s) 693, 2974
BstMCI CGRYCG 2 cut(s) 2310, 2314
BstNI CCWGG 4 cut(s) 980, 1629, 2586, 2921
BstNSI RCATGY 2 cut(s) 799, 2949
BstSCI CCNGG 7 cut(s) 48, 135, 410, 978, 1627, 2584, 2919
BstSFI CTRYAG 2 cut(s) 1425, 2521
BstSLI GKGCMC 3 cut(s) 515, 1960, 2306
BstV2I GAAGAC 3 cut(s) 435, 500, 990
BstX2I RGATCY 1 cut(s) 2974
BstXI CCANNNNNNTGG 3 cut(s) 1457, 2222, 2882
BstYI RGATCY 1 cut(s) 2974
BstZI CGGCCG 2 cut(s) 2307, 2311
BsuRI GGCC 8 cut(s) 32, 36, 188, 361, 1532, 1958, 2309, 2313
BtsCI GGATG 4 cut(s) 251, 298, 1017, 2655
BtsI GCAGTG 8 cut(s) 548, 768, 987, 1599, 1626, 1653, 2037, 2184
BveI ACCTGC 3 cut(s) 158, 1830, 2371
CaiI CAGNNNCTG 4 cut(s) 574, 946, 1090, 2143
CciI TCATGA 7 cut(s) 1023, 1332, 1662, 1797, 1941, 2076, 2511
CfoI GCGC 5 cut(s) 329, 1063, 1210, 1587, 2233
Cfr10I RCCGGY 6 cut(s) 32, 208, 1357, 1687, 2309, 2313
CseI GACGC 1 cut(s) 1714
CsiI ACCWGGT 1 cut(s) 1627
Csp6I GTAC 6 cut(s) 257, 598, 1537, 1698, 2817, 2962
CviQI GTAC 6 cut(s) 257, 598, 1537, 1698, 2817, 2962
DpnI GATC 2 cut(s) 695, 2976
DpnII GATC 2 cut(s) 693, 2974
DraI TTTAAA 1 cut(s) 2839
DraIII CACNNNGTG 1 cut(s) 521
EaeI YGGCCR 6 cut(s) 30, 186, 359, 1530, 2307, 2311
EagI CGGCCG 2 cut(s) 2307, 2311
Eam1104I CTCTTC 3 cut(s) 96, 305, 2406
EarI CTCTTC 3 cut(s) 96, 305, 2406
EciI GGCGGA 2 cut(s) 1564, 2662
Ecl136II GAGCTC 3 cut(s) 1803, 2082, 2517
EclXI CGGCCG 2 cut(s) 2307, 2311
Eco130I CCWWGG 5 cut(s) 372, 559, 1495, 2260, 2797
Eco24I GRGCYC 9 cut(s) 860, 1424, 1475, 1754, 1805, 1960, 2084, 2519, 2548
Eco31I GGTCTC 4 cut(s) 217, 727, 2765, 2979
Eco52I CGGCCG 2 cut(s) 2307, 2311
Eco53kI GAGCTC 3 cut(s) 1803, 2082, 2517
Eco57I CTGAAG 2 cut(s) 447, 2871
Eco88I CYCGRG 1 cut(s) 2239
EcoICRI GAGCTC 3 cut(s) 1803, 2082, 2517
EcoNI CCTNNNNNAGG 1 cut(s) 92
EcoRII CCWGG 4 cut(s) 978, 1627, 2584, 2919
EcoT14I CCWWGG 5 cut(s) 372, 559, 1495, 2260, 2797
EcoT22I ATGCAT 1 cut(s) 2947
EcoT38I GRGCYC 9 cut(s) 860, 1424, 1475, 1754, 1805, 1960, 2084, 2519, 2548
ErhI CCWWGG 5 cut(s) 372, 559, 1495, 2260, 2797
FalI AAGNNNNNCTT 2 cut(s) 1549, 1581
FauI CCCGC 4 cut(s) 329, 1482, 2753, 2907
FauNDI CATATG 1 cut(s) 1511
FokI GGATG 4 cut(s) 258, 305, 1004, 2662
FriOI GRGCYC 9 cut(s) 860, 1424, 1475, 1754, 1805, 1960, 2084, 2519, 2548
FseI GGCCGGCC 2 cut(s) 36, 2313
FspBI CTAG 2 cut(s) 89, 776
FspI TGCGCA 2 cut(s) 328, 2232
GlaI GCGC 5 cut(s) 328, 1062, 1209, 1586, 2232
GsaI CCCAGC 1 cut(s) 63
GsuI CTGGAG 1 cut(s) 1244
HaeII RGCGCY 2 cut(s) 1064, 1211
HaeIII GGCC 8 cut(s) 32, 36, 188, 361, 1532, 1958, 2309, 2313
HgaI GACGC 1 cut(s) 1714
HhaI GCGC 5 cut(s) 329, 1063, 1210, 1587, 2233
Hin6I GCGC 5 cut(s) 327, 1061, 1208, 1585, 2231
HinP1I GCGC 5 cut(s) 327, 1061, 1208, 1585, 2231
HincII GTYRAC 1 cut(s) 1819
HindII GTYRAC 1 cut(s) 1819
HinfI GANTC 2 cut(s) 535, 1349
HphI GGTGA 7 cut(s) 478, 502, 742, 1193, 1643, 1677, 2352
Hpy166II GTNNAC 7 cut(s) 513, 1539, 1819, 2304, 2379, 2734, 2819
Hpy188I TCNGA 4 cut(s) 933, 1714, 2663, 2767
Hpy8I GTNNAC 7 cut(s) 513, 1539, 1819, 2304, 2379, 2734, 2819
HpyAV CCTTC 1 cut(s) 676
HpyCH4IV ACGT 1 cut(s) 647
HpySE526I ACGT 1 cut(s) 647
HspAI GCGC 5 cut(s) 327, 1061, 1208, 1585, 2231
KflI GGGWCCC 5 cut(s) 368, 554, 1038, 1490, 2654
KroI GCCGGC 3 cut(s) 32, 2309, 2313
KroNI GCCGGC 3 cut(s) 34, 2311, 2315
Kzo9I GATC 2 cut(s) 693, 2974
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 9 cut(s) 166, 251, 820, 937, 1225, 1384, 1808, 1984, 2522
LweI GCATC 2 cut(s) 1766, 1782
MabI ACCWGGT 1 cut(s) 1627
MaeI CTAG 2 cut(s) 89, 776
MaeII ACGT 1 cut(s) 647
MalI GATC 2 cut(s) 695, 2976
MbiI CCGCTC 1 cut(s) 2991
MboI GATC 2 cut(s) 693, 2974
MfeI CAATTG 1 cut(s) 2780
MflI RGATCY 1 cut(s) 2974
MlsI TGGCCA 2 cut(s) 188, 1532
MluNI TGGCCA 2 cut(s) 188, 1532
MlyI GAGTC 2 cut(s) 529, 1343
MmeI TCCRAC 2 cut(s) 144, 2320
Mox20I TGGCCA 2 cut(s) 188, 1532
Mph1103I ATGCAT 1 cut(s) 2947
MroNI GCCGGC 3 cut(s) 32, 2309, 2313
MscI TGGCCA 2 cut(s) 188, 1532
MseI TTAA 6 cut(s) 228, 453, 477, 501, 1005, 2838
MslI CAYNNNNRTG 5 cut(s) 815, 1022, 1331, 2075, 2286
Msp20I TGGCCA 2 cut(s) 188, 1532
MspA1I CMGCKG 4 cut(s) 946, 1090, 1237, 2143
MspR9I CCNGG 7 cut(s) 50, 137, 412, 980, 1629, 2586, 2921
MunI CAATTG 1 cut(s) 2780
Mva1269I GAATGC 3 cut(s) 664, 1387, 3025
MvaI CCWGG 4 cut(s) 980, 1629, 2586, 2921
NaeI GCCGGC 3 cut(s) 34, 2311, 2315
NciI CCSGG 3 cut(s) 50, 137, 412
NdeI CATATG 1 cut(s) 1511
NdeII GATC 2 cut(s) 693, 2974
NgoMIV GCCGGC 3 cut(s) 32, 2309, 2313
NmeAIII GCCGAG 5 cut(s) 220, 300, 1765, 2479, 3008
NmuCI GTSAC 5 cut(s) 6, 458, 482, 808, 1868
NsbI TGCGCA 2 cut(s) 328, 2232
NsiI ATGCAT 1 cut(s) 2947
NspI RCATGY 2 cut(s) 799, 2949
NspV TTCGAA 1 cut(s) 668
OliI CACNNNNGTG 1 cut(s) 815
PagI TCATGA 7 cut(s) 1023, 1332, 1662, 1797, 1941, 2076, 2511
PaqCI CACCTGC 1 cut(s) 2371
PasI CCCWGGG 1 cut(s) 2920
PciI ACATGT 1 cut(s) 795
PciSI GCTCTTC 1 cut(s) 96
PctI GAATGC 3 cut(s) 664, 1387, 3025
PdiI GCCGGC 3 cut(s) 34, 2311, 2315
PleI GAGTC 2 cut(s) 529, 1343
PpsI GAGTC 2 cut(s) 529, 1343
PscI ACATGT 1 cut(s) 795
PsiI TTATAA 1 cut(s) 1056
Psp124BI GAGCTC 3 cut(s) 1805, 2084, 2519
Psp6I CCWGG 4 cut(s) 978, 1627, 2584, 2919
PspFI CCCAGC 1 cut(s) 59
PspGI CCWGG 4 cut(s) 978, 1627, 2584, 2919
PspOMI GGGCCC 1 cut(s) 1956
PstI CTGCAG 1 cut(s) 2525
PstNI CAGNNNCTG 4 cut(s) 574, 946, 1090, 2143
PsuI RGATCY 1 cut(s) 2974
PvuII CAGCTG 4 cut(s) 946, 1090, 1237, 2143
RigI GGCCGGCC 2 cut(s) 36, 2313
RsaI GTAC 6 cut(s) 258, 599, 1538, 1699, 2818, 2963
RsaNI GTAC 6 cut(s) 257, 598, 1537, 1698, 2817, 2962
RseI CAYNNNNRTG 5 cut(s) 815, 1022, 1331, 2075, 2286
SacI GAGCTC 3 cut(s) 1805, 2084, 2519
SapI GCTCTTC 1 cut(s) 96
SaqAI TTAA 6 cut(s) 228, 453, 477, 501, 1005, 2838
Sau3AI GATC 2 cut(s) 693, 2974
SbfI CCTGCAGG 1 cut(s) 2525
ScaI AGTACT 1 cut(s) 258
SchI GAGTC 2 cut(s) 529, 1343
ScrFI CCNGG 7 cut(s) 50, 137, 412, 980, 1629, 2586, 2921
SdaI CCTGCAGG 1 cut(s) 2525
SexAI ACCWGGT 1 cut(s) 1627
SfaNI GCATC 2 cut(s) 1766, 1782
SfcI CTRYAG 2 cut(s) 1425, 2521
SfuI TTCGAA 1 cut(s) 668
SmiMI CAYNNNNRTG 5 cut(s) 815, 1022, 1331, 2075, 2286
SmlI CTYRAG 5 cut(s) 902, 1043, 1190, 1961, 3013
SmoI CTYRAG 5 cut(s) 902, 1043, 1190, 1961, 3013
Sse8387I CCTGCAGG 1 cut(s) 2525
SspMI CTAG 2 cut(s) 89, 776
SstI GAGCTC 3 cut(s) 1805, 2084, 2519
StyD4I CCNGG 7 cut(s) 48, 135, 410, 978, 1627, 2584, 2919
StyI CCWWGG 5 cut(s) 372, 559, 1495, 2260, 2797
TaiI ACGT 1 cut(s) 650
TaqI TCGA 5 cut(s) 144, 668, 1365, 1703, 2387
TatI WGTACW 3 cut(s) 256, 1536, 2816
TauI GCSGC 6 cut(s) 268, 418, 764, 767, 1198, 2724
Tru1I TTAA 6 cut(s) 228, 453, 477, 501, 1005, 2838
Tru9I TTAA 6 cut(s) 228, 453, 477, 501, 1005, 2838
TseFI GTSAC 5 cut(s) 6, 458, 482, 808, 1868
Tsp45I GTSAC 5 cut(s) 6, 458, 482, 808, 1868
TspDTI ATGAA 5 cut(s) 800, 1157, 1316, 1349, 1679
TspGWI ACGGA 2 cut(s) 176, 2552
VneI GTGCAC 2 cut(s) 511, 2302
XagI CCTNNNNNAGG 1 cut(s) 92
XapI RAATTY 5 cut(s) 926, 1844, 1988, 2123, 2966
XceI RCATGY 2 cut(s) 799, 2949
XcmI CCANNNNNNNNNTGG 3 cut(s) 1306, 2162, 2851
XspI CTAG 2 cut(s) 89, 776
ZrmI AGTACT 1 cut(s) 258
Zsp2I ATGCAT 1 cut(s) 2947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.