Rh4DG125600

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000654
Physical Location & Seq
Forward (+)
20402127 .. 20407720
5594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG125600.1

Sequence Viewer

Length: 3594 bp
ATGAGCTCACAGTTGGATGCTTCTTCTTCTTCTTCCTCGTCCTCCCCTTACCGGTGTACTTATCATGCATTCTTGAGTTTCAGAGGTGAGGACACACGTAAGGGATTTACCGATCATCTTTACAGGGCCTTGGAGCTCGCAGGAATCCACACGTTTAGAGACGATGATGAAATCGAGAGAGGAGCAAATATCGCAGCAGAGTTACAGAGAGCTATCCAAGAGTCGAGGGTATCTGTGGTTGTGTTGTCTAGAGACTATGCTTCATCAAGGTGGTGCTTGGACGAACTTGCAAAGATCATGGAACGTAGGAGAAATGATGACGCACACGTGGTTATTCCAGTGTTTTATGATGTAGATCCATCCCATGTCAGGAAGCAAATCGGGAGTTTTGCTCAAGCTTTTGTTAGACATGAAGAGCGCTTCAAGGAGGAGATGGGAAAGGTTGCCGAGTGGAGAAGAGCGCTTTCTGATATTGCAGATCTTGGTGGAATGGTTTTAGGACAACGGTATGAATCACAATTTATCCAAGATATTGTTGAAGAAATCAGAAGTAAATTGGATTCCAAAGTCTTAAACATTGCTCCGTATGCAATTGGAATAGATGATCGTGTGCGAGGTATAAACATGTGGTTAGAAGATGGGTCAAATGATGTTGGTGTGGCTGTGATCTATGGAATGGGTGGAATTGGCAAAACCACTATTGCCAAAGCTGCTTATAACTTGAATTTCCATAGATTTCAAGGTAGCAGCTTTCTTGCTGATGTTAGAGAAACTTCAGAACAGCCGAATGGTTTGGTTCGCTTGCAAAGAAATCTTCTTTCAGATCTCCAAAAGGGGAAAACAAAGAAAATATACAACATTGATGAAGGAATACGAAAGATAAAAGATGCTGTATGTTGCAAGAAAGTTCTTATTGTTCTTGATGATGTGAACCACTCAGACCAATTTAATGCAATTATTGGAATGCGCAGATGGTTTGTTCCAGGAAGTAAGATAATCTTAACAACCAGATACGAACATTTGTTAAAGGCTCACGAAGTTAACACAATGTTTAAGGTTCAAGAGTTGAATGAGCATGAATCACTTGAGCTCCTCAGTTGGCACGCCTTTGGACAAGCCCATCCTATTGAAGCTTACATTGATCTTTCAAAACCCGTTGTAAAACACTGTGGAGGGATTCCATTAGCTCTGCAAGTTCTGGGATCTTCTTTGTCTGGCAGAAGTATAGATATATGGCAAAGCGCATTACAGAAGCTATGTGAGATTCCAGATGGAGAAATTCAAAAAATTCTTAGAATCAGTTTTGACTCTCTGCAAGATGATCATGACAAAAACTTATTCCTTCATATAGCATGCTTCTTCAGGGGAAAGAATAAAGAGTTTTCAATTACAGTACTGGACAACCTTAAATTCTACACAGTGATTGGAATTCAAACTCTGGTTGATAGATGTCTAGTGAAAATTAATGAAGACAACAAGTTGATAGTGCATCATTTGCTTCGAGACATGGCAAGGGGAATTATTCGCGAAGAATCACCTGAGGACCCTGGGAAACGCAGTAGGGTGTGGCACAAAGATGCCTTCAATATTTTGAGTAAAAGAACTGGTACTGGAACTGTTAAGGGCATCATGCTTGACCTTTCTATGTTGATGCAAGATGAGTCTTCCAAAACATTATTTAGTGGAAGTAACAGCAAACGACGTCGTGTTGATGATTATGATGGAAATTGTTCAGGACGGCGTCGACTTGGTCTCTTCACCTGGCAATTATTTAGTTTTTCCTCAGCCAACACGACTCCAGCATCAGATGAGGTTGACCTCAAAATAGAGGCATTCAAAAAGATGCACAACCTTGAAGTTCTCCTACTCAATAATGTACATTTCAGTCAAGGCTTTGAAAATTTTCCAAAAAACTTGATATGGCTGTCTTGGCGAGGATTCTCTTTAAATTCGATACCAGCCAGTTTGTATTTGAAAACTCTAGTTGTTCTTGACTTGCGGAATAGCAGCCTGCAATGTGTTTGGAAGGGAACCAAGTCTCTTCCAAGTCTGAAAGTCCTTAATCTTAGTCATTCACATGGCCTTTCAGCAACCCCCGACATATCAGGACTCCCTAACCTGGAGAGATTGATTCTAAAAAATTGTAGAAATTTGATTGAGGTTGATGAATCGATTGGAGACTTGGAGAAACTTGTTTTCTTAAATCTAAAAGACTGCAAAAATCTGATGAAGCTTCCGACCAGAATCAAAATGTCAAGTTCTCTTCAAACACTTATTCTTTCTGGTTGCTCAAAGCTTGTGCTTCATTCTAATATGGTTGCAACAAATCAGTCACTCTTGACAGCTAGTGATATGAAGAAAGACGATTGTTCATCACCTAACTTGTGGAAATCACTCTTATTATGGGCAGTACCAAGCAGAAATATCATACCAACCAGTTTCTCAGTATCAAATTTACCACGTTCTTTACGAAGCTTAAGCTTAGCTGACTGCAACCTATTAGAGATTCCAGGTGATCTTAGTATCCTCTCCTCATTGAAGTATTTAGACCTATGTGGGAACCCAATTCAGAGCCTCCCAGAAAACATAAGATCTCTTAGTAAGCTTGAGACACTTATGTTAGAGCACTGCACAGAACTCAGAATGCTTCCAGAGCTCCCACCAAGTCTCCAGAGTTTGCTTGCAAGTTCTTGTAAATCATTGAAAAGAATAACAAATTTACCAAACTTGTTCAAATCATTGGATACAGATTTTCTGGATTGCAAGAAGTTAGTTGAAGTTGAAAGCTTGTTCACTATAAAACCATTGACAACCACTGACATAGAAATGATCAAAGATATGGGACTGTTCAATTTTAAATCCATTGGAAGCGACGCTGAGGTTGAAATGATCAACTACATTACAAATACAACCAAGAAGGTCCCCCTCCAGGTGTTGTCTGAATGTGGCATATCTAGCATTTACCTAAACGGAAACGAGATTCCAGATTGGTTTCGCCACAGGAGAATGGGCAACTCTGTGTTGTCTATTATAGTACCTCCACATCTTAATCATAGAATTCGAGGCTTAAATGCATGTGTTGTATATGCACGACGCCCAGATATTCGTCGTTCTGCTAAATATCAAAACTTGTTTAAAGTCAGCAATGAGACCAAGGGTCTCATGTGGACCTATTCCCCTATCACAATAGGCATTCCAAAGGAGAATGAAGACATGTTATGGCTAAGCCATTGGCGGTTTGGAGAACATGATCTGGGAGGGGGAGACGAAGTACGTGTTTCGGTTGAGTTTGGACCCGAGCTTGGACACAATCTAAATTTATGGACAAAGGAATTGGGAATCCAACTTGTGTATGAGCAGGAAAGTAGTGAAGATGGAAAGAAACACAATTTCCTTGCTGGAGATGTGTCAGTGTCATCCTCAAGTTACCAAATGTGGACGGGAAAATACTTTCTTTGTAATAACCTGAATCGTGCTCAGAAAACACAATTCAAGAGTAGCCAGGAAAACCCATCTCATCTTGATTCTGACAAAACCAAGAGCCCCTTCCTTTTCTTTTTTGAGCATGATGATGTTTCACAGGCTGATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1197

Amino Acids

135.54

Weight (kDa)

7.76

Isoelectric Point (pI)

46.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 189 7.8e-55 TIR domain
TIR_2 PF13676 24 - 134 2.7e-18 TIR domain
NB-ARC PF00931 202 - 370 5.3e-20 NB-ARC domain
WHD_ROQ1 PF23282 441 - 512 1.9e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 651 - 762 7.1e-06 Leucine-rich repeat region
LRR_14 PF23598 821 - 914 2.2e-08 Leucine-rich repeat region
LRR_8 PF13855 821 - 877 8.3e-07 Leucine rich repeat
C-JID PF20160 990 - 1122 2.5e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 717
AatII GACGTC 1 cut(s) 1705
Acc16I TGCGCA 1 cut(s) 968
AccI GTMKAC 1 cut(s) 1744
AccII CGCG 1 cut(s) 1527
AciI CCGC 2 cut(s) 1999, 3235
AclWI GGATC 2 cut(s) 350, 1210
AcuI CTGAAG 2 cut(s) 759, 1345
AcvI CACGTG 1 cut(s) 328
AcyI GRCGYC 3 cut(s) 1702, 1741, 3094
AfaI GTAC 7 cut(s) 58, 1395, 1609, 1878, 2412, 3036, 3273
AfeI AGCGCT 2 cut(s) 419, 462
AfiI CCNNNNNNNGG 5 cut(s) 51, 369, 2119, 2929, 3302
AflII CTTAAG 1 cut(s) 2476
AflIII ACRYGT 6 cut(s) 95, 150, 325, 624, 3213, 3274
AgeI ACCGGT 1 cut(s) 51
AhdI GACNNNNNGTC 1 cut(s) 3156
AjnI CCWGG 7 cut(s) 982, 1546, 1760, 2118, 2509, 2928, 3501
AjuI GAANNNNNNNTTGG 2 cut(s) 3530, 3562
Alw21I GWGCWC 6 cut(s) 8, 138, 1092, 2628, 2658, 3478
AlwI GGATC 2 cut(s) 350, 1210
Ama87I CYCGRG 1 cut(s) 3296
Aor51HI AGCGCT 2 cut(s) 419, 462
AoxI GGCC 2 cut(s) 126, 2080
ApeKI GCWGC 4 cut(s) 194, 710, 747, 2007
AseI ATTAAT 1 cut(s) 1464
AsiGI ACCGGT 1 cut(s) 51
Asp700I GAANNNNTTC 2 cut(s) 1729, 1902
AspLEI GCGC 4 cut(s) 420, 463, 969, 1244
AspS9I GGNCC 5 cut(s) 126, 1543, 2920, 3168, 3293
AsuHPI GGTGA 5 cut(s) 98, 1527, 1750, 2367, 2525
AvaI CYCGRG 1 cut(s) 3296
AvaII GGWCC 4 cut(s) 1543, 2920, 3168, 3293
AxyI CCTNAGG 1 cut(s) 1539
BanII GRGCYC 5 cut(s) 8, 138, 1092, 2658, 3545
BarI GAAGNNNNNNTAC 2 cut(s) 1848, 1880
BbrPI CACGTG 1 cut(s) 328
BbsI GAAGAC 3 cut(s) 1476, 1656, 3216
Bbv12I GWGCWC 6 cut(s) 8, 138, 1092, 2628, 2658, 3478
BbvCI CCTCAGC 2 cut(s) 1783, 2877
BbvI GCAGC 4 cut(s) 206, 697, 759, 2019
BccI CCATC 9 cut(s) 367, 427, 632, 966, 1128, 1265, 1715, 3368, 3520
BceAI ACGGC 1 cut(s) 1754
BciT130I CCWGG 7 cut(s) 984, 1548, 1762, 2120, 2511, 2930, 3503
BciVI GTATCC 2 cut(s) 2534, 2737
BclI TGATCA 4 cut(s) 1321, 2829, 2889, 3586
BfaI CTAG 5 cut(s) 249, 1454, 1982, 2346, 2955
BfoI RGCGCY 2 cut(s) 421, 464
BfrI CTTAAG 1 cut(s) 2476
BfuI GTATCC 2 cut(s) 2534, 2737
BglII AGATCT 3 cut(s) 478, 823, 2591
BisI GCNGC 4 cut(s) 195, 711, 748, 2008
BlpI GCTNAGC 2 cut(s) 2482, 3224
BlsI GCNGC 4 cut(s) 196, 712, 749, 2009
BmcAI AGTACT 1 cut(s) 1395
Bme1390I CCNGG 7 cut(s) 984, 1548, 1762, 2120, 2511, 2930, 3503
Bme18I GGWCC 4 cut(s) 1543, 2920, 3168, 3293
BmeRI GACNNNNNGTC 1 cut(s) 3156
BmeT110I CYCGRG 1 cut(s) 3296
BmgT120I GGNCC 5 cut(s) 126, 1543, 2920, 3168, 3293
BmiI GGNNCC 5 cut(s) 1545, 2032, 2561, 2922, 3295
BmrFI CCNGG 7 cut(s) 984, 1548, 1762, 2120, 2511, 2930, 3503
BmsI GCATC 8 cut(s) 7, 877, 1498, 1567, 1635, 1641, 1811, 1833
BpiI GAAGAC 3 cut(s) 1476, 1656, 3216
BplI GAGNNNNNCTC 2 cut(s) 376, 408
BpmI CTGGAG 5 cut(s) 1782, 2141, 2654, 2912, 3420
Bpu10I CCTNAGC 2 cut(s) 1783, 2877
Bpu1102I GCTNAGC 2 cut(s) 2482, 3224
BpuEI CTTGAG 5 cut(s) 94, 378, 1106, 2627, 3406
Bsa29I ATCGAT 1 cut(s) 2171
BsaAI YACGTR 3 cut(s) 98, 328, 3275
BsaBI GATNNNNATC 1 cut(s) 354
BsaHI GRCGYC 3 cut(s) 1702, 1741, 3094
BsaI GGTCTC 3 cut(s) 1757, 3143, 3164
BsaJI CCNNGG 4 cut(s) 129, 1546, 1547, 3153
BsaWI WCCGGW 1 cut(s) 51
BsaXI ACNNNNNCTCC 6 cut(s) 1074, 1104, 2652, 2682, 3255, 3285
Bsc4I CCNNNNNNNGG 5 cut(s) 51, 369, 2119, 2929, 3302
Bse118I RCCGGY 1 cut(s) 51
Bse1I ACTGG 6 cut(s) 338, 1401, 1609, 1615, 1962, 2436
Bse21I CCTNAGG 1 cut(s) 1539
Bse3DI GCAATG 3 cut(s) 576, 2021, 3151
Bse8I GATNNNNATC 1 cut(s) 354
BseBI CCWGG 7 cut(s) 984, 1548, 1762, 2120, 2511, 2930, 3503
BseCI ATCGAT 1 cut(s) 2171
BseDI CCNNGG 4 cut(s) 129, 1546, 1547, 3153
BseGI GGATG 4 cut(s) 22, 359, 1120, 3416
BseJI GATNNNNATC 1 cut(s) 354
BseLI CCNNNNNNNGG 5 cut(s) 51, 369, 2119, 2929, 3302
BseMI GCAATG 3 cut(s) 576, 2021, 3151
BseMII CTCAG 8 cut(s) 951, 1108, 1530, 1797, 2457, 2653, 2868, 3491
BseNI ACTGG 6 cut(s) 338, 1401, 1609, 1615, 1962, 2436
BseRI GAGGAG 4 cut(s) 195, 443, 1082, 2521
BseXI GCAGC 4 cut(s) 206, 697, 759, 2019
BsgI GTGCAG 1 cut(s) 2614
Bsh1236I CGCG 1 cut(s) 1527
BshFI GGCC 2 cut(s) 128, 2082
BshTI ACCGGT 1 cut(s) 51
BshVI ATCGAT 1 cut(s) 2171
BsiHKAI GWGCWC 6 cut(s) 8, 138, 1092, 2628, 2658, 3478
BsiHKCI CYCGRG 1 cut(s) 3296
BsiSI CCGG 1 cut(s) 52
BslFI GGGAC 2 cut(s) 2856, 2906
BslI CCNNNNNNNGG 5 cut(s) 51, 369, 2119, 2929, 3302
BsmBI CGTCTC 2 cut(s) 153, 3258
BsmFI GGGAC 2 cut(s) 2856, 2906
BsmI GAATGC 5 cut(s) 68, 969, 1832, 2649, 3192
BsnI GGCC 2 cut(s) 128, 2082
Bso31I GGTCTC 3 cut(s) 1757, 3143, 3164
BsoBI CYCGRG 1 cut(s) 3296
Bsp1286I GDGCHC 7 cut(s) 8, 138, 1092, 2628, 2658, 3478, 3545
Bsp1407I TGTACA 1 cut(s) 1876
Bsp1720I GCTNAGC 2 cut(s) 2482, 3224
Bsp68I TCGCGA 1 cut(s) 1527
BspACI CCGC 2 cut(s) 1999, 3235
BspANI GGCC 2 cut(s) 128, 2082
BspCNI CTCAG 8 cut(s) 950, 1107, 1531, 1796, 2456, 2652, 2869, 3490
BspDI ATCGAT 1 cut(s) 2171
BspFNI CGCG 1 cut(s) 1527
BspHI TCATGA 1 cut(s) 1324
BspLI GGNNCC 5 cut(s) 1545, 2032, 2561, 2922, 3295
BspPI GGATC 2 cut(s) 350, 1210
BspQI GCTCTTC 2 cut(s) 408, 451
BspTI CTTAAG 1 cut(s) 2476
BspTNI GGTCTC 3 cut(s) 1757, 3143, 3164
BsrDI GCAATG 3 cut(s) 576, 2021, 3151
BsrFI RCCGGY 1 cut(s) 51
BsrGI TGTACA 1 cut(s) 1876
BsrI ACTGG 6 cut(s) 338, 1401, 1609, 1615, 1962, 2436
BssAI RCCGGY 1 cut(s) 51
BssECI CCNNGG 4 cut(s) 129, 1546, 1547, 3153
BssNI GRCGYC 3 cut(s) 1702, 1741, 3094
BssT1I CCWWGG 2 cut(s) 129, 3153
Bst2UI CCWGG 7 cut(s) 984, 1548, 1762, 2120, 2511, 2930, 3503
Bst4CI ACNGT 7 cut(s) 12, 507, 1169, 1393, 1420, 1618, 2847
Bst6I CTCTTC 5 cut(s) 408, 451, 1760, 2046, 2268
BstACI GRCGYC 3 cut(s) 1702, 1741, 3094
BstAFI CTTAAG 1 cut(s) 2476
BstAPI GCANNNNNTGC 1 cut(s) 1495
BstAUI TGTACA 1 cut(s) 1876
BstBAI YACGTR 3 cut(s) 98, 328, 3275
BstC8I GCNNGC 6 cut(s) 138, 803, 1104, 1354, 2012, 2682
BstF5I GGATG 4 cut(s) 22, 359, 1120, 3416
BstFNI CGCG 1 cut(s) 1527
BstH2I RGCGCY 2 cut(s) 421, 464
BstHHI GCGC 4 cut(s) 420, 463, 969, 1244
BstMWI GCNNNNNNNGC 7 cut(s) 191, 587, 710, 1495, 1930, 2653, 2955
BstNI CCWGG 7 cut(s) 984, 1548, 1762, 2120, 2511, 2930, 3503
BstNSI RCATGY 4 cut(s) 628, 1356, 3078, 3217
BstSCI CCNGG 7 cut(s) 982, 1546, 1760, 2118, 2509, 2928, 3501
BstUI CGCG 1 cut(s) 1527
BstV1I GCAGC 4 cut(s) 206, 697, 759, 2019
BstV2I GAAGAC 3 cut(s) 1476, 1656, 3216
BstX2I RGATCY 5 cut(s) 355, 478, 823, 1202, 2591
BstYI RGATCY 5 cut(s) 355, 478, 823, 1202, 2591
Bsu15I ATCGAT 1 cut(s) 2171
Bsu36I CCTNAGG 1 cut(s) 1539
BsuI GTATCC 2 cut(s) 2534, 2737
BsuRI GGCC 2 cut(s) 128, 2082
BsuTUI ATCGAT 1 cut(s) 2171
BtsCI GGATG 4 cut(s) 22, 359, 1120, 3416
BtsI GCAGTG 1 cut(s) 2626
BtsIMutI CAGTG 6 cut(s) 345, 1165, 1425, 2626, 2814, 3417
BtuMI TCGCGA 1 cut(s) 1527
Cac8I GCNNGC 6 cut(s) 138, 803, 1104, 1354, 2012, 2682
CciI TCATGA 1 cut(s) 1324
CfoI GCGC 4 cut(s) 420, 463, 969, 1244
Cfr10I RCCGGY 1 cut(s) 51
Cfr13I GGNCC 5 cut(s) 126, 1543, 2920, 3168, 3293
ClaI ATCGAT 1 cut(s) 2171
CseI GACGC 4 cut(s) 329, 1730, 2882, 3102
Csp6I GTAC 7 cut(s) 57, 1394, 1608, 1877, 2411, 3035, 3272
CspAI ACCGGT 1 cut(s) 51
CviQI GTAC 7 cut(s) 57, 1394, 1608, 1877, 2411, 3035, 3272
DraI TTTAAA 3 cut(s) 1947, 2857, 3136
DriI GACNNNNNGTC 1 cut(s) 3156
Eam1104I CTCTTC 5 cut(s) 408, 451, 1760, 2046, 2268
Eam1105I GACNNNNNGTC 1 cut(s) 3156
EarI CTCTTC 5 cut(s) 408, 451, 1760, 2046, 2268
Ecl136II GAGCTC 4 cut(s) 6, 136, 1090, 2656
Eco130I CCWWGG 2 cut(s) 129, 3153
Eco24I GRGCYC 5 cut(s) 8, 138, 1092, 2658, 3545
Eco31I GGTCTC 3 cut(s) 1757, 3143, 3164
Eco47I GGWCC 4 cut(s) 1543, 2920, 3168, 3293
Eco47III AGCGCT 2 cut(s) 419, 462
Eco53kI GAGCTC 4 cut(s) 6, 136, 1090, 2656
Eco57I CTGAAG 2 cut(s) 759, 1345
Eco72I CACGTG 1 cut(s) 328
Eco81I CCTNAGG 1 cut(s) 1539
Eco88I CYCGRG 1 cut(s) 3296
EcoICRI GAGCTC 4 cut(s) 6, 136, 1090, 2656
EcoO109I RGGNCCY 3 cut(s) 126, 1543, 2920
EcoRI GAATTC 2 cut(s) 1428, 3057
EcoRII CCWGG 7 cut(s) 982, 1546, 1760, 2118, 2509, 2928, 3501
EcoT14I CCWWGG 2 cut(s) 129, 3153
EcoT22I ATGCAT 2 cut(s) 70, 3076
EcoT38I GRGCYC 5 cut(s) 8, 138, 1092, 2658, 3545
ErhI CCWWGG 2 cut(s) 129, 3153
Esp3I CGTCTC 2 cut(s) 153, 3258
FalI AAGNNNNNCTT 4 cut(s) 983, 1015, 3530, 3562
FaqI GGGAC 2 cut(s) 2856, 2906
FbaI TGATCA 4 cut(s) 1321, 2829, 2889, 3586
FblI GTMKAC 1 cut(s) 1744
Fnu4HI GCNGC 4 cut(s) 195, 711, 748, 2008
FokI GGATG 4 cut(s) 29, 346, 1107, 3403
FriOI GRGCYC 5 cut(s) 8, 138, 1092, 2658, 3545
Fsp4HI GCNGC 4 cut(s) 195, 711, 748, 2008
FspBI CTAG 5 cut(s) 249, 1454, 1982, 2346, 2955
FspI TGCGCA 1 cut(s) 968
GlaI GCGC 4 cut(s) 419, 462, 968, 1243
GluI GCNGC 4 cut(s) 195, 711, 748, 2008
GsuI CTGGAG 5 cut(s) 1782, 2141, 2654, 2912, 3420
HaeII RGCGCY 2 cut(s) 421, 464
HaeIII GGCC 2 cut(s) 128, 2082
HapII CCGG 1 cut(s) 52
HgaI GACGC 4 cut(s) 329, 1730, 2882, 3102
HhaI GCGC 4 cut(s) 420, 463, 969, 1244
Hin1I GRCGYC 3 cut(s) 1702, 1741, 3094
Hin6I GCGC 4 cut(s) 418, 461, 967, 1242
HinP1I GCGC 4 cut(s) 418, 461, 967, 1242
HincII GTYRAC 3 cut(s) 1042, 1745, 1816
HindII GTYRAC 3 cut(s) 1042, 1745, 1816
HindIII AAGCTT 8 cut(s) 396, 1131, 2231, 2294, 2473, 2479, 2603, 2785
HpaI GTTAAC 1 cut(s) 1042
HpaII CCGG 1 cut(s) 52
HphI GGTGA 5 cut(s) 98, 1527, 1750, 2367, 2525
Hpy166II GTNNAC 8 cut(s) 57, 931, 1042, 1745, 1816, 2793, 3168, 3438
Hpy8I GTNNAC 8 cut(s) 57, 931, 1042, 1745, 1816, 2793, 3168, 3438
Hpy99I CGWCG 6 cut(s) 1704, 1707, 1746, 2876, 3096, 3111
HpyAV CCTTC 6 cut(s) 860, 1352, 1591, 2020, 2911, 3556
HpyCH4III ACNGT 7 cut(s) 12, 507, 1169, 1393, 1420, 1618, 2847
HpyCH4IV ACGT 7 cut(s) 97, 152, 304, 327, 1702, 2461, 3274
HpyF10VI GCNNNNNNNGC 7 cut(s) 191, 587, 710, 1495, 1930, 2653, 2955
HpySE526I ACGT 7 cut(s) 97, 152, 304, 327, 1702, 2461, 3274
Hsp92I GRCGYC 3 cut(s) 1702, 1741, 3094
HspAI GCGC 4 cut(s) 418, 461, 967, 1242
Ksp22I TGATCA 4 cut(s) 1321, 2829, 2889, 3586
KspAI GTTAAC 1 cut(s) 1042
LguI GCTCTTC 2 cut(s) 408, 451
LmnI GCTCC 5 cut(s) 133, 182, 586, 1095, 2661
Lsp1109I GCAGC 4 cut(s) 206, 697, 759, 2019
LweI GCATC 8 cut(s) 7, 877, 1498, 1567, 1635, 1641, 1811, 1833
MaeI CTAG 5 cut(s) 249, 1454, 1982, 2346, 2955
MaeII ACGT 7 cut(s) 97, 152, 304, 327, 1702, 2461, 3274
MaeIII GTNAC 4 cut(s) 201, 1688, 2331, 3425
MfeI CAATTG 1 cut(s) 591
MflI RGATCY 5 cut(s) 355, 478, 823, 1202, 2591
MhlI GDGCHC 7 cut(s) 8, 138, 1092, 2628, 2658, 3478, 3545
MlyI GAGTC 5 cut(s) 230, 1301, 1670, 1789, 2103
MmeI TCCRAC 2 cut(s) 2261, 3367
Mph1103I ATGCAT 2 cut(s) 70, 3076
MroXI GAANNNNTTC 2 cut(s) 1729, 1902
MslI CAYNNNNRTG 5 cut(s) 268, 1575, 2076, 2825, 3570
MspCI CTTAAG 1 cut(s) 2476
MspI CCGG 1 cut(s) 52
MspR9I CCNGG 7 cut(s) 984, 1548, 1762, 2120, 2511, 2930, 3503
MunI CAATTG 1 cut(s) 591
Mva1269I GAATGC 5 cut(s) 68, 969, 1832, 2649, 3192
MvaI CCWGG 7 cut(s) 984, 1548, 1762, 2120, 2511, 2930, 3503
MvnI CGCG 1 cut(s) 1527
MwoI GCNNNNNNNGC 7 cut(s) 191, 587, 710, 1495, 1930, 2653, 2955
NlaIV GGNNCC 5 cut(s) 1545, 2032, 2561, 2922, 3295
NmeAIII GCCGAG 1 cut(s) 472
NmuCI GTSAC 1 cut(s) 2331
NruI TCGCGA 1 cut(s) 1527
NsbI TGCGCA 1 cut(s) 968
NsiI ATGCAT 2 cut(s) 70, 3076
NspI RCATGY 4 cut(s) 628, 1356, 3078, 3217
PaeI GCATGC 1 cut(s) 1356
PagI TCATGA 1 cut(s) 1324
PasI CCCWGGG 1 cut(s) 1547
PciI ACATGT 2 cut(s) 624, 3213
PciSI GCTCTTC 2 cut(s) 408, 451
PcsI WCGNNNNNNNCGW 1 cut(s) 2467
PctI GAATGC 5 cut(s) 68, 969, 1832, 2649, 3192
PdmI GAANNNNTTC 2 cut(s) 1729, 1902
PflFI GACNNNGTC 2 cut(s) 1740, 1749
PfoI TCCNGGA 1 cut(s) 982
PinAI ACCGGT 1 cut(s) 51
PkrI GCNGC 4 cut(s) 196, 712, 749, 2009
PleI GAGTC 5 cut(s) 229, 1301, 1669, 1789, 2103
PmaCI CACGTG 1 cut(s) 328
PmlI CACGTG 1 cut(s) 328
PpsI GAGTC 5 cut(s) 229, 1301, 1669, 1789, 2103
Ppu21I YACGTR 3 cut(s) 98, 328, 3275
PpuMI RGGWCCY 2 cut(s) 1543, 2920
PscI ACATGT 2 cut(s) 624, 3213
PshBI ATTAAT 1 cut(s) 1464
PsiI TTATAA 1 cut(s) 717
Psp124BI GAGCTC 4 cut(s) 8, 138, 1092, 2658
Psp5II RGGWCCY 2 cut(s) 1543, 2920
Psp6I CCWGG 7 cut(s) 982, 1546, 1760, 2118, 2509, 2928, 3501
PspCI CACGTG 1 cut(s) 328
PspGI CCWGG 7 cut(s) 982, 1546, 1760, 2118, 2509, 2928, 3501
PspN4I GGNNCC 5 cut(s) 1545, 2032, 2561, 2922, 3295
PspPI GGNCC 5 cut(s) 126, 1543, 2920, 3168, 3293
PspPPI RGGWCCY 2 cut(s) 1543, 2920
PsuI RGATCY 5 cut(s) 355, 478, 823, 1202, 2591
PsyI GACNNNGTC 2 cut(s) 1740, 1749
RruI TCGCGA 1 cut(s) 1527
RsaI GTAC 7 cut(s) 58, 1395, 1609, 1878, 2412, 3036, 3273
RsaNI GTAC 7 cut(s) 57, 1394, 1608, 1877, 2411, 3035, 3272
RseI CAYNNNNRTG 5 cut(s) 268, 1575, 2076, 2825, 3570
SacI GAGCTC 4 cut(s) 8, 138, 1092, 2658
SalI GTCGAC 1 cut(s) 1743
SapI GCTCTTC 2 cut(s) 408, 451
SatI GCNGC 4 cut(s) 195, 711, 748, 2008
Sau96I GGNCC 5 cut(s) 126, 1543, 2920, 3168, 3293
ScaI AGTACT 1 cut(s) 1395
SchI GAGTC 5 cut(s) 230, 1301, 1670, 1789, 2103
ScrFI CCNGG 7 cut(s) 984, 1548, 1762, 2120, 2511, 2930, 3503
SduI GDGCHC 7 cut(s) 8, 138, 1092, 2628, 2658, 3478, 3545
SfaNI GCATC 8 cut(s) 7, 877, 1498, 1567, 1635, 1641, 1811, 1833
SinI GGWCC 4 cut(s) 1543, 2920, 3168, 3293
SmiMI CAYNNNNRTG 5 cut(s) 268, 1575, 2076, 2825, 3570
SmlI CTYRAG 6 cut(s) 73, 393, 1085, 2476, 2606, 3421
SmoI CTYRAG 6 cut(s) 73, 393, 1085, 2476, 2606, 3421
SphI GCATGC 1 cut(s) 1356
SsiI CCGC 2 cut(s) 1999, 3235
SspI AATATT 1 cut(s) 1588
SspMI CTAG 5 cut(s) 249, 1454, 1982, 2346, 2955
SstI GAGCTC 4 cut(s) 8, 138, 1092, 2658
StyD4I CCNGG 7 cut(s) 982, 1546, 1760, 2118, 2509, 2928, 3501
StyI CCWWGG 2 cut(s) 129, 3153
TaaI ACNGT 7 cut(s) 12, 507, 1169, 1393, 1420, 1618, 2847
TaiI ACGT 7 cut(s) 100, 155, 307, 330, 1705, 2464, 3277
TaqI TCGA 7 cut(s) 174, 224, 1501, 1744, 1952, 2171, 3061
TatI WGTACW 3 cut(s) 56, 1393, 1876
TscAI CASTG 6 cut(s) 345, 1172, 1425, 2633, 2821, 3417
TseFI GTSAC 1 cut(s) 2331
TseI GCWGC 4 cut(s) 194, 710, 747, 2007
Tsp45I GTSAC 1 cut(s) 2331
TspGWI ACGGA 2 cut(s) 573, 2985
TspRI CASTG 6 cut(s) 345, 1172, 1425, 2633, 2821, 3417
Tth111I GACNNNGTC 2 cut(s) 1740, 1749
Vha464I CTTAAG 1 cut(s) 2476
VpaK11BI GGWCC 4 cut(s) 1543, 2920, 3168, 3293
VspI ATTAAT 1 cut(s) 1464
XbaI TCTAGA 1 cut(s) 248
XceI RCATGY 4 cut(s) 628, 1356, 3078, 3217
XcmI CCANNNNNNNNNTGG 1 cut(s) 3236
XmiI GTMKAC 1 cut(s) 1744
XmnI GAANNNNTTC 2 cut(s) 1729, 1902
XspI CTAG 5 cut(s) 249, 1454, 1982, 2346, 2955
ZraI GACGTC 1 cut(s) 1703
ZrmI AGTACT 1 cut(s) 1395
Zsp2I ATGCAT 2 cut(s) 70, 3076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.