MD17G1277700.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000654
Physical Location & Seq
Reverse (-)
33771885 .. 33776182
4298 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1277700.v1.1.491

Sequence Viewer

Length: 3594 bp
ATGGCTGCAATAATTGCTCAGCTCGATGCCTCTTCCACCTCCTACCGGTGTACTTATGATGTGTTCTTGAGTTACAGAGGCGAAGATACACGTAAGGGGTTTACTGATCACCTCTACAGGGAGCTGGAGCTGGCAGGAATTCACACCTTTAGAGATGATGATGGGATCGACAGAGGAGCAAACATAGCATCAGAGCTACAGAAAGCGATACAAGAATCACGAGCATCCATAATTGTCTTCTCCAAGGACTACGCTTCCTCAAGATGGTGCTTGGATGAGCTGGTGAAGATCATGAAACGTAGAAAAACTGATTGCAGACACATGGTTATGCCAGTTTTCTATGATGTGGATCCATCCCATGTTAGGAAACAGACTGGAACTTTTGCACAAGCATTTGCTAGACATGAAGAACGCTTCAAGGAGGAGATGGACAAGGTGATGGAGTGGAGAAGAGCTCTTAGAGATGTAGCAGATGTGGGAGGAATGGTTTTAGGAGATATGTATGAGTCACAGTTTATCCAAAATATTGTCGAAGAGATTGGAAATAAACTGGATCATTGTGCAACAGAAATGATTGCTCCCTATGTGGTTGGAATAGATAAGCGTGTGCAGGGCATAAACATGTGGCTGAAAGATCGATCAAATGACGTGAGTGTAGCTGTGATCTATGGGATGGGTGGAATTGGCAAGACCACCATTGCCAAAGCTGCTTATAACCAGAACTTCCACAGATTTCAAGGTTGCAGCTATCTTGCAGATGTTAGAGCAACTTCAGAACAGCCTAATGGTTTGGCTTTTTTGCAAAGAAAACTTCTTTCGGATATCCAAAAGGGGAAAAAGAAGAAAGTATACAGTATGGGTGAACGAATGAGCAAGATCAAGCATGCTGTGTGTTGCAAAACTGTTCTTATTGTTCTTGATGATGTGGACCAATCTGACCAATTCAATGCAATTCTTGGAAGGCGAGAATGGTTTCACCCAGGAAGTAAAATAATCGTTACAACTAGACATGAAAGTTTGTTGAATGATCATGAAGTTTATGCAATGTTTAAGGTTAGAGGATTGGGTGAGTATGAATCACTTGAGCTTTTTAGTTGGCATGCCTTCAAACAAGTGCGTCCTGTCAAAGATTACATCAAGCTAACAAGATCCGTAGTACAGCACTGTCAAGGGCTACCATTAGCACTTCAAGTTTTGGGATCTTCTCTTGTTGGAAGAAGTGTAGAAGTATGGGAAAGTGCGCTGCAGAAGTTAAACGTCATTCCTAATGAAAAAATTCAAAAAATACTTAGAATAAGCTTTGATTCTCTACAAGATGATCATGACAGAAGTTTATTCCTTCATATAGTCTCTTTCTTCGTGGGAAAGAAGATAGATTATACAATTACAGTACTGGACAATTTACACTTCTACACAAGGATTGGAATTCAAAATCTAGTTGATAGATGTCTAGTAGACATAGATGAAGACAACAAGTTGGTCATGCATCAATTACTTATAGACATGGGAAGGGGAATTATTCGTGAAGAGTCGCCCGAGGACCCTGGGAAACGTAGTATAGTGCTACAGAATGATGCCTCTGATATTTTGAGAAAATTAACTGGAACAAAAACCATTAAGGGCCTCATGCTCAATCTTCCTGGCAAGACAATGTTTAATACAAGTAACAAAAAGAGATACCATGTCGACGATTACCATGAAAAAGTCTCAAGGCGACGGCTTGGTTTCTTCTCTTGGAAATCTATTACCTTTCTGCCAACAAATTCTGCTTCTGAATCAAATGAGGTAGACTTCAAAACAGAGGCATTTACAAGGATGCACAATCTTGAACTTCTGTTGCTCAATAATGTAATCATCAGTGGAGGCTACAAAGATTTTCCAAAAAATTTAAAATGGATATCTTGGCGAGTATTCCCTTTAAAATCAATACCAGCAGATTTATATTTGGAAAATCTAGTGGGTCTTGACTTGCGGAATAGCAGGCTACATCATCTTTGGCAAGGACCCAGGGTTATTCCAAGACTGAAATTCCTTAATGTCAGTCATTCACATGGTCTTGTGAGAACCCCCAACTTTTCGGGAGTCCCTAACATTGAGAAATTAGTTCTTAAAGATTGCACAAATTTGGTTTTGGTTGATGAATCCATTGCAGACCTAGAGAAACTCATTGTCTTGAATCTAAAAGACTGCAAAAATCTCCCAAAGCTTCCAACAAGACTTAGCATGATGACATCTCTACAGGAACTGATTCTTTCTGGTTGCTCAAAGCTTGTGTTTCGCCCCAATGCTGCAGCTGGTGTTTGGAAGAAACTCAATATATCAACAGCTAAGCTATCGCAATCAATGTGGTTGTGGAGACTGTGGATATTACTCAGGAAACATCTTGTTACTGCCCGCAGTTTGTCAGCGGCAAATTGGCCAAATGGCTTAGTAAGCTTAAGTCTAGCTGACTGCAATCTTCCGGAGATTCCCAGTGGTCTTAGTATCCTATCTTCATTGAAGCATTTGAATCTAAGTAGAAACCCAATTTTGAGCCTACCAGAAAACTTGAATGATCTTATTACGCTTCAAACTCTTGTAATTGAAGGTTGCACAATGGTCCGAACTCTTCCAGAACTCCCACCAAGTTTAAGAAAATTGCATGCAAGTTATTGTACATCATTGGAAAAAATAACAAATTTACCAAACATGTTCAGATCATTGGATTCAAGTTTTTGGAAGTGCAAGAATTTAGTTGAAGTTCAAAGCTTGTTCAATATAAAACCATTGAGAAGGGTTGACATTGAAATGATCAGAGATATGGGCCTGTTCAATTTGGAATCCGTAGGAACAACTGAAGTTGAGATGACCAATTACTTGACACGTACAAGAAGGAAGGGTCCTATACAGGGATTGTATGAATGTGGCATATTTAGCGCTTTCCTTCACGGAAACAAGATTCCAGATAGGTTCCCTTACAGAAGCCTGGGTAACTCCACTTTGTCTATTATTGTACCTTCACATCTTAATCTCAAGGTCCGAGGCTTGAATGCTTGTGTTGTATATTCGCGATGTCCTTTTTGGTTTAGCAGTTCAAACTTCCTTAAAGTCAGTAATGAGACCAAGGGTCTTAAATGGACATATTGCCCAGTCAGAGCAGGTCTCCCGAGAAAGAACCATGATATGTTATGGCTAAGCCATTGGTTGTTTGGAAACGATGAACTGGAGGGCGGGGATGAAGTACGTTTTTCCATGAATGAAGAATATAGTTTCTGGACGAAGGAATTCGGTATCCAGCTTGTGTATGAGCAGGAAAATGAGGGGAAGGGTGTTCAATCAAAAAGTGAAGATATGACATTCCAACCAAATACAACTCTTTCTAGCCCGGTTTTTGTTGCTGGAAATGTATCTGCGTCAGCATCAAAGTATCAGTTGTGGACGGGCAAATTCTTTCTTTGCAATCATCGGAGTCGTATTCATCAACTTCATTTTAGGAGTCAGGAGAGACCAGATTATCTTGGTTTTTCATACTTATTTAAGGAAGATGTTCCTGCTAAGACGATGGTTCCATCTACTCCAATGAATGTTACACCGATTCCTTTTACGTGGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1198

Amino Acids

136.93

Weight (kDa)

9.12

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 19 - 189 4.1e-54 TIR domain
TIR_2 PF13676 21 - 133 2.1e-17 TIR domain
NB-ARC PF00931 201 - 370 4.7e-20 NB-ARC domain
WHD_ROQ1 PF23282 441 - 511 3.2e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 826 - 930 1.9e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
Acc36I ACCTGC 1 cut(s) 3128
AccI GTMKAC 4 cut(s) 849, 1455, 1686, 1788
AccII CGCG 1 cut(s) 3049
AccIII TCCGGA 1 cut(s) 2461
AciI CCGC 4 cut(s) 1972, 2395, 2408, 3210
AclWI GGATC 6 cut(s) 173, 344, 357, 561, 1143, 1207
AcoI YGGCCR 1 cut(s) 2417
AcuI CTGAAG 2 cut(s) 756, 2856
AfaI GTAC 7 cut(s) 52, 1158, 1392, 2656, 2866, 2994, 3222
AfeI AGCGCT 1 cut(s) 2917
AfiI CCNNNNNNNGG 5 cut(s) 45, 118, 264, 363, 2888
AflII CTTAAG 1 cut(s) 2437
AflIII ACRYGT 4 cut(s) 89, 621, 2688, 2861
AgeI ACCGGT 1 cut(s) 45
AhdI GACNNNNNGTC 1 cut(s) 3105
AjiI CACGTC 1 cut(s) 649
AjnI CCWGG 5 cut(s) 979, 1543, 1639, 2006, 2964
Alw21I GWGCWC 1 cut(s) 457
Alw26I GTCTC 6 cut(s) 1354, 1711, 2350, 3092, 3146, 3478
AlwI GGATC 6 cut(s) 173, 344, 357, 561, 1143, 1207
AlwNI CAGNNNCTG 1 cut(s) 2245
Ama87I CYCGRG 2 cut(s) 1535, 3145
Aor13HI TCCGGA 1 cut(s) 2461
Aor51HI AGCGCT 1 cut(s) 2917
AoxI GGCC 3 cut(s) 1621, 2417, 2803
ApeKI GCWGC 6 cut(s) 5, 707, 744, 1243, 2288, 2291
AsiGI ACCGGT 1 cut(s) 45
Asp700I GAANNNNTTC 5 cut(s) 972, 1275, 2247, 3263, 3525
AspLEI GCGC 2 cut(s) 1243, 2918
AspS9I GGNCC 8 cut(s) 928, 1540, 1621, 2003, 2599, 2803, 2879, 3016
AsuC2I CCSGG 1 cut(s) 3365
AsuHPI GGTGA 6 cut(s) 101, 295, 448, 872, 968, 1079
AvaI CYCGRG 2 cut(s) 1535, 3145
AvaII GGWCC 6 cut(s) 928, 1540, 2003, 2599, 2879, 3016
BalI TGGCCA 1 cut(s) 2419
BamHI GGATCC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 457
BarI GAAGNNNNNNTAC 4 cut(s) 833, 865, 2476, 2508
BauI CACGAG 1 cut(s) 219
BbsI GAAGAC 2 cut(s) 229, 1473
Bbv12I GWGCWC 1 cut(s) 457
BbvI GCAGC 5 cut(s) 694, 756, 1230, 2275, 2303
BccI CCATC 8 cut(s) 155, 258, 361, 421, 433, 667, 3535, 3556
BceAI ACGGC 1 cut(s) 1733
BciT130I CCWGG 5 cut(s) 981, 1545, 1641, 2008, 2966
BciVI GTATCC 2 cut(s) 2495, 3281
BclI TGATCA 4 cut(s) 106, 1027, 1318, 2790
BcnI CCSGG 1 cut(s) 3365
BcoDI GTCTC 6 cut(s) 1354, 1711, 2350, 3092, 3146, 3478
BfaI CTAG 8 cut(s) 399, 1005, 1436, 1451, 1955, 2156, 2444, 3360
BfmI CTRYAG 6 cut(s) 115, 197, 1244, 1565, 2237, 2289
BfoI RGCGCY 1 cut(s) 2919
BfrI CTTAAG 1 cut(s) 2437
BfuAI ACCTGC 1 cut(s) 3128
BfuI GTATCC 2 cut(s) 2495, 3281
BisI GCNGC 7 cut(s) 6, 708, 745, 1244, 2289, 2292, 2409
BlpI GCTNAGC 3 cut(s) 18, 2328, 3173
BlsI GCNGC 7 cut(s) 7, 709, 746, 1245, 2290, 2293, 2410
BmcAI AGTACT 1 cut(s) 1392
Bme1390I CCNGG 6 cut(s) 981, 1545, 1641, 2008, 2966, 3365
Bme18I GGWCC 6 cut(s) 928, 1540, 2003, 2599, 2879, 3016
BmeRI GACNNNNNGTC 1 cut(s) 3105
BmeT110I CYCGRG 2 cut(s) 1535, 3145
BmgBI CACGTC 1 cut(s) 649
BmgT120I GGNCC 8 cut(s) 928, 1540, 1621, 2003, 2599, 2803, 2879, 3016
BmiI GGNNCC 6 cut(s) 351, 1542, 2005, 2880, 2951, 3546
BmrFI CCNGG 6 cut(s) 981, 1545, 1641, 2008, 2966, 3365
BmrI ACTGGG 2 cut(s) 2466, 3122
BmsI GCATC 7 cut(s) 16, 197, 233, 1495, 1564, 1806, 3407
BmuI ACTGGG 2 cut(s) 2466, 3122
BpiI GAAGAC 2 cut(s) 229, 1473
BplI GAGNNNNNCTC 4 cut(s) 439, 471, 3126, 3158
BpmI CTGGAG 2 cut(s) 146, 3224
Bpu1102I GCTNAGC 3 cut(s) 18, 2328, 3173
BpuEI CTTGAG 5 cut(s) 88, 244, 1103, 1693, 2996
BpuMI CCSGG 1 cut(s) 3365
Bsa29I ATCGAT 1 cut(s) 637
BsaAI YACGTR 3 cut(s) 92, 2864, 3585
BsaBI GATNNNNATC 1 cut(s) 348
BsaI GGTCTC 3 cut(s) 3092, 3146, 3478
BsaWI WCCGGW 2 cut(s) 45, 2461
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 5 cut(s) 45, 118, 264, 363, 2888
Bse118I RCCGGY 1 cut(s) 45
Bse1I ACTGG 8 cut(s) 332, 379, 555, 1398, 1606, 2472, 3128, 3207
Bse3DI GCAATG 3 cut(s) 696, 1050, 2145
Bse8I GATNNNNATC 1 cut(s) 348
BseAI TCCGGA 1 cut(s) 2461
BseBI CCWGG 5 cut(s) 981, 1545, 1641, 2008, 2966
BseCI ATCGAT 1 cut(s) 637
BseGI GGATG 6 cut(s) 224, 280, 353, 678, 1821, 3220
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 5 cut(s) 45, 118, 264, 363, 2888
BseMI GCAATG 3 cut(s) 696, 1050, 2145
BseMII CTCAG 2 cut(s) 32, 2386
BseNI ACTGG 8 cut(s) 332, 379, 555, 1398, 1606, 2472, 3128, 3207
BseRI GAGGAG 2 cut(s) 189, 437
BseXI GCAGC 5 cut(s) 694, 756, 1230, 2275, 2303
BsgI GTGCAG 1 cut(s) 629
Bsh1236I CGCG 1 cut(s) 3049
BshFI GGCC 3 cut(s) 1623, 2419, 2805
BshTI ACCGGT 1 cut(s) 45
BshVI ATCGAT 1 cut(s) 637
BsiHKAI GWGCWC 1 cut(s) 457
BsiHKCI CYCGRG 2 cut(s) 1535, 3145
BsiSI CCGG 3 cut(s) 46, 2462, 3365
BslFI GGGAC 1 cut(s) 2069
BslI CCNNNNNNNGG 5 cut(s) 45, 118, 264, 363, 2888
BsmAI GTCTC 6 cut(s) 1354, 1711, 2350, 3092, 3146, 3478
BsmFI GGGAC 1 cut(s) 2069
BsmI GAATGC 1 cut(s) 3034
BsnI GGCC 3 cut(s) 1623, 2419, 2805
Bso31I GGTCTC 3 cut(s) 3092, 3146, 3478
BsoBI CYCGRG 2 cut(s) 1535, 3145
Bsp1286I GDGCHC 1 cut(s) 457
Bsp13I TCCGGA 1 cut(s) 2461
Bsp1407I TGTACA 1 cut(s) 2654
Bsp1720I GCTNAGC 3 cut(s) 18, 2328, 3173
Bsp68I TCGCGA 1 cut(s) 3049
BspACI CCGC 4 cut(s) 1972, 2395, 2408, 3210
BspANI GGCC 3 cut(s) 1623, 2419, 2805
BspCNI CTCAG 2 cut(s) 31, 2385
BspDI ATCGAT 1 cut(s) 637
BspEI TCCGGA 1 cut(s) 2461
BspFNI CGCG 1 cut(s) 3049
BspHI TCATGA 3 cut(s) 291, 1030, 1321
BspLI GGNNCC 6 cut(s) 351, 1542, 2005, 2880, 2951, 3546
BspMAI CTGCAG 2 cut(s) 1248, 2293
BspMI ACCTGC 1 cut(s) 3128
BspPI GGATC 6 cut(s) 173, 344, 357, 561, 1143, 1207
BspQI GCTCTTC 1 cut(s) 445
BspTI CTTAAG 1 cut(s) 2437
BspTNI GGTCTC 3 cut(s) 3092, 3146, 3478
BsrDI GCAATG 3 cut(s) 696, 1050, 2145
BsrFI RCCGGY 1 cut(s) 45
BsrGI TGTACA 1 cut(s) 2654
BsrI ACTGG 8 cut(s) 332, 379, 555, 1398, 1606, 2472, 3128, 3207
BssAI RCCGGY 1 cut(s) 45
BssNAI GTATAC 1 cut(s) 850
BssSI CACGAG 1 cut(s) 219
BssT1I CCWWGG 2 cut(s) 243, 3102
Bst1107I GTATAC 1 cut(s) 850
Bst2BI CACGAG 1 cut(s) 219
Bst2UI CCWGG 5 cut(s) 981, 1545, 1641, 2008, 2966
Bst4CI ACNGT 6 cut(s) 513, 854, 904, 1166, 1390, 2361
Bst6I CTCTTC 5 cut(s) 37, 445, 528, 1521, 2613
BstAFI CTTAAG 1 cut(s) 2437
BstAPI GCANNNNNTGC 1 cut(s) 14
BstAUI TGTACA 1 cut(s) 2654
BstBAI YACGTR 3 cut(s) 92, 2864, 3585
BstC8I GCNNGC 6 cut(s) 132, 885, 1101, 1982, 2395, 2643
BstF5I GGATG 6 cut(s) 224, 280, 353, 678, 1821, 3220
BstFNI CGCG 1 cut(s) 3049
BstH2I RGCGCY 1 cut(s) 2919
BstHHI GCGC 2 cut(s) 1243, 2918
BstMAI GTCTC 6 cut(s) 1354, 1711, 2350, 3092, 3146, 3478
BstMWI GCNNNNNNNGC 5 cut(s) 14, 185, 707, 2433, 2913
BstNI CCWGG 5 cut(s) 981, 1545, 1641, 2008, 2966
BstNSI RCATGY 5 cut(s) 625, 887, 1103, 2645, 2692
BstSCI CCNGG 6 cut(s) 979, 1543, 1639, 2006, 2964, 3363
BstSFI CTRYAG 6 cut(s) 115, 197, 1244, 1565, 2237, 2289
BstUI CGCG 1 cut(s) 3049
BstV1I GCAGC 5 cut(s) 694, 756, 1230, 2275, 2303
BstV2I GAAGAC 2 cut(s) 229, 1473
BstX2I RGATCY 3 cut(s) 349, 1148, 1199
BstYI RGATCY 3 cut(s) 349, 1148, 1199
BstZ17I GTATAC 1 cut(s) 850
Bsu15I ATCGAT 1 cut(s) 637
BsuI GTATCC 2 cut(s) 2495, 3281
BsuRI GGCC 3 cut(s) 1623, 2419, 2805
BsuTUI ATCGAT 1 cut(s) 637
BtgZI GCGATG 1 cut(s) 3064
BtrI CACGTC 1 cut(s) 649
BtsCI GGATG 6 cut(s) 224, 280, 353, 678, 1821, 3220
BtsIMutI CAGTG 3 cut(s) 1162, 1864, 2479
BtuMI TCGCGA 1 cut(s) 3049
BveI ACCTGC 1 cut(s) 3128
Cac8I GCNNGC 6 cut(s) 132, 885, 1101, 1982, 2395, 2643
CaiI CAGNNNCTG 1 cut(s) 2245
CciI TCATGA 3 cut(s) 291, 1030, 1321
CfoI GCGC 2 cut(s) 1243, 2918
Cfr10I RCCGGY 1 cut(s) 45
Cfr13I GGNCC 8 cut(s) 928, 1540, 1621, 2003, 2599, 2803, 2879, 3016
ClaI ATCGAT 1 cut(s) 637
CseI GACGC 2 cut(s) 1106, 3381
Csp6I GTAC 7 cut(s) 51, 1157, 1391, 2655, 2865, 2993, 3221
CspAI ACCGGT 1 cut(s) 45
CspCI CAANNNNNGTGG 2 cut(s) 2327, 2362
CviQI GTAC 7 cut(s) 51, 1157, 1391, 2655, 2865, 2993, 3221
DraI TTTAAA 2 cut(s) 1890, 1920
DriI GACNNNNNGTC 1 cut(s) 3105
EaeI YGGCCR 1 cut(s) 2417
Eam1104I CTCTTC 5 cut(s) 37, 445, 528, 1521, 2613
Eam1105I GACNNNNNGTC 1 cut(s) 3105
EarI CTCTTC 5 cut(s) 37, 445, 528, 1521, 2613
Ecl136II GAGCTC 1 cut(s) 455
Eco130I CCWWGG 2 cut(s) 243, 3102
Eco24I GRGCYC 1 cut(s) 457
Eco31I GGTCTC 3 cut(s) 3092, 3146, 3478
Eco32I GATATC 2 cut(s) 823, 1899
Eco47I GGWCC 6 cut(s) 928, 1540, 2003, 2599, 2879, 3016
Eco47III AGCGCT 1 cut(s) 2917
Eco53kI GAGCTC 1 cut(s) 455
Eco57I CTGAAG 2 cut(s) 756, 2856
Eco88I CYCGRG 2 cut(s) 1535, 3145
EcoICRI GAGCTC 1 cut(s) 455
EcoO109I RGGNCCY 4 cut(s) 1540, 1621, 2003, 2879
EcoRI GAATTC 3 cut(s) 138, 1425, 3263
EcoRII CCWGG 5 cut(s) 979, 1543, 1639, 2006, 2964
EcoRV GATATC 2 cut(s) 823, 1899
EcoT14I CCWWGG 2 cut(s) 243, 3102
EcoT22I ATGCAT 1 cut(s) 1488
EcoT38I GRGCYC 1 cut(s) 457
ErhI CCWWGG 2 cut(s) 243, 3102
FaqI GGGAC 1 cut(s) 2069
FauI CCCGC 2 cut(s) 2402, 3203
FbaI TGATCA 4 cut(s) 106, 1027, 1318, 2790
FblI GTMKAC 4 cut(s) 849, 1455, 1686, 1788
Fnu4HI GCNGC 7 cut(s) 6, 708, 745, 1244, 2289, 2292, 2409
FokI GGATG 6 cut(s) 211, 287, 340, 685, 1828, 3227
FriOI GRGCYC 1 cut(s) 457
Fsp4HI GCNGC 7 cut(s) 6, 708, 745, 1244, 2289, 2292, 2409
FspBI CTAG 8 cut(s) 399, 1005, 1436, 1451, 1955, 2156, 2444, 3360
GlaI GCGC 2 cut(s) 1242, 2917
GluI GCNGC 7 cut(s) 6, 708, 745, 1244, 2289, 2292, 2409
GsuI CTGGAG 2 cut(s) 146, 3224
HaeII RGCGCY 1 cut(s) 2919
HaeIII GGCC 3 cut(s) 1623, 2419, 2805
HapII CCGG 3 cut(s) 46, 2462, 3365
HgaI GACGC 2 cut(s) 1106, 3381
HhaI GCGC 2 cut(s) 1243, 2918
Hin6I GCGC 2 cut(s) 1241, 2916
HinP1I GCGC 2 cut(s) 1241, 2916
HincII GTYRAC 2 cut(s) 1687, 2779
HindII GTYRAC 2 cut(s) 1687, 2779
HindIII AAGCTT 5 cut(s) 1297, 2204, 2267, 2434, 2746
HpaII CCGG 3 cut(s) 46, 2462, 3365
HphI GGTGA 6 cut(s) 101, 295, 448, 872, 968, 1079
Hpy99I CGWCG 2 cut(s) 1691, 1719
HpyCH4III ACNGT 6 cut(s) 513, 854, 904, 1166, 1390, 2361
HpyCH4IV ACGT 8 cut(s) 91, 298, 648, 1257, 1552, 2863, 3223, 3584
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 185, 707, 2433, 2913
HpySE526I ACGT 8 cut(s) 91, 298, 648, 1257, 1552, 2863, 3223, 3584
HspAI GCGC 2 cut(s) 1241, 2916
Kpn2I TCCGGA 1 cut(s) 2461
Ksp22I TGATCA 4 cut(s) 106, 1027, 1318, 2790
LguI GCTCTTC 1 cut(s) 445
LmnI GCTCC 4 cut(s) 121, 127, 176, 583
Lsp1109I GCAGC 5 cut(s) 694, 756, 1230, 2275, 2303
LweI GCATC 7 cut(s) 16, 197, 233, 1495, 1564, 1806, 3407
MaeI CTAG 8 cut(s) 399, 1005, 1436, 1451, 1955, 2156, 2444, 3360
MaeII ACGT 8 cut(s) 91, 298, 648, 1257, 1552, 2863, 3223, 3584
MaeIII GTNAC 7 cut(s) 71, 507, 997, 1664, 2386, 2969, 3565
MflI RGATCY 3 cut(s) 349, 1148, 1199
MhlI GDGCHC 1 cut(s) 457
MlsI TGGCCA 1 cut(s) 2419
MluNI TGGCCA 1 cut(s) 2419
MlyI GAGTC 5 cut(s) 515, 1538, 2091, 3457, 3484
MmeI TCCRAC 4 cut(s) 571, 1192, 2234, 3364
Mox20I TGGCCA 1 cut(s) 2419
Mph1103I ATGCAT 1 cut(s) 1488
MroI TCCGGA 1 cut(s) 2461
MroXI GAANNNNTTC 5 cut(s) 972, 1275, 2247, 3263, 3525
MscI TGGCCA 1 cut(s) 2419
MslI CAYNNNNRTG 4 cut(s) 326, 620, 2049, 2786
Msp20I TGGCCA 1 cut(s) 2419
MspA1I CMGCKG 2 cut(s) 2294, 2408
MspCI CTTAAG 1 cut(s) 2437
MspI CCGG 3 cut(s) 46, 2462, 3365
MspR9I CCNGG 6 cut(s) 981, 1545, 1641, 2008, 2966, 3365
Mva1269I GAATGC 1 cut(s) 3034
MvaI CCWGG 5 cut(s) 981, 1545, 1641, 2008, 2966
MvnI CGCG 1 cut(s) 3049
MwoI GCNNNNNNNGC 5 cut(s) 14, 185, 707, 2433, 2913
NciI CCSGG 1 cut(s) 3365
NlaIV GGNNCC 6 cut(s) 351, 1542, 2005, 2880, 2951, 3546
NmuCI GTSAC 1 cut(s) 507
NruI TCGCGA 1 cut(s) 3049
NsiI ATGCAT 1 cut(s) 1488
NspI RCATGY 5 cut(s) 625, 887, 1103, 2645, 2692
PaeI GCATGC 3 cut(s) 887, 1103, 2645
PagI TCATGA 3 cut(s) 291, 1030, 1321
PasI CCCWGGG 2 cut(s) 1544, 2007
PciI ACATGT 2 cut(s) 621, 2688
PciSI GCTCTTC 1 cut(s) 445
PctI GAATGC 1 cut(s) 3034
PdmI GAANNNNTTC 5 cut(s) 972, 1275, 2247, 3263, 3525
PinAI ACCGGT 1 cut(s) 45
PkrI GCNGC 7 cut(s) 7, 709, 746, 1245, 2290, 2293, 2410
PleI GAGTC 5 cut(s) 514, 1537, 2090, 3456, 3483
PpsI GAGTC 5 cut(s) 514, 1537, 2090, 3456, 3483
Ppu21I YACGTR 3 cut(s) 92, 2864, 3585
PpuMI RGGWCCY 3 cut(s) 1540, 2003, 2879
PscI ACATGT 2 cut(s) 621, 2688
PsiI TTATAA 1 cut(s) 714
Psp124BI GAGCTC 1 cut(s) 457
Psp5II RGGWCCY 3 cut(s) 1540, 2003, 2879
Psp6I CCWGG 5 cut(s) 979, 1543, 1639, 2006, 2964
PspGI CCWGG 5 cut(s) 979, 1543, 1639, 2006, 2964
PspN4I GGNNCC 6 cut(s) 351, 1542, 2005, 2880, 2951, 3546
PspPI GGNCC 8 cut(s) 928, 1540, 1621, 2003, 2599, 2803, 2879, 3016
PspPPI RGGWCCY 3 cut(s) 1540, 2003, 2879
PstI CTGCAG 2 cut(s) 1248, 2293
PstNI CAGNNNCTG 1 cut(s) 2245
PsuI RGATCY 3 cut(s) 349, 1148, 1199
PvuII CAGCTG 1 cut(s) 2294
RruI TCGCGA 1 cut(s) 3049
RsaI GTAC 7 cut(s) 52, 1158, 1392, 2656, 2866, 2994, 3222
RsaNI GTAC 7 cut(s) 51, 1157, 1391, 2655, 2865, 2993, 3221
RseI CAYNNNNRTG 4 cut(s) 326, 620, 2049, 2786
SacI GAGCTC 1 cut(s) 457
SalI GTCGAC 1 cut(s) 1685
SapI GCTCTTC 1 cut(s) 445
SatI GCNGC 7 cut(s) 6, 708, 745, 1244, 2289, 2292, 2409
Sau96I GGNCC 8 cut(s) 928, 1540, 1621, 2003, 2599, 2803, 2879, 3016
ScaI AGTACT 1 cut(s) 1392
SchI GAGTC 5 cut(s) 515, 1538, 2091, 3457, 3484
ScrFI CCNGG 6 cut(s) 981, 1545, 1641, 2008, 2966, 3365
SduI GDGCHC 1 cut(s) 457
SfaNI GCATC 7 cut(s) 16, 197, 233, 1495, 1564, 1806, 3407
SfcI CTRYAG 6 cut(s) 115, 197, 1244, 1565, 2237, 2289
SinI GGWCC 6 cut(s) 928, 1540, 2003, 2599, 2879, 3016
SmiMI CAYNNNNRTG 4 cut(s) 326, 620, 2049, 2786
SmlI CTYRAG 6 cut(s) 67, 259, 1082, 1708, 2437, 3011
SmoI CTYRAG 6 cut(s) 67, 259, 1082, 1708, 2437, 3011
SphI GCATGC 3 cut(s) 887, 1103, 2645
SsiI CCGC 4 cut(s) 1972, 2395, 2408, 3210
SspI AATATT 1 cut(s) 526
SspMI CTAG 8 cut(s) 399, 1005, 1436, 1451, 1955, 2156, 2444, 3360
SstI GAGCTC 1 cut(s) 457
StyD4I CCNGG 6 cut(s) 979, 1543, 1639, 2006, 2964, 3363
StyI CCWWGG 2 cut(s) 243, 3102
TaaI ACNGT 6 cut(s) 513, 854, 904, 1166, 1390, 2361
TaiI ACGT 8 cut(s) 94, 301, 651, 1260, 1555, 2866, 3226, 3587
TaqI TCGA 5 cut(s) 24, 168, 531, 637, 1686
TatI WGTACW 4 cut(s) 50, 1156, 1390, 2654
TauI GCSGC 1 cut(s) 2411
TscAI CASTG 3 cut(s) 1169, 1864, 2479
TseFI GTSAC 1 cut(s) 507
TseI GCWGC 6 cut(s) 5, 707, 744, 1243, 2288, 2291
Tsp45I GTSAC 1 cut(s) 507
TspGWI ACGGA 3 cut(s) 1141, 2812, 2943
TspRI CASTG 3 cut(s) 1169, 1864, 2479
Vha464I CTTAAG 1 cut(s) 2437
VpaK11BI GGWCC 6 cut(s) 928, 1540, 2003, 2599, 2879, 3016
XceI RCATGY 5 cut(s) 625, 887, 1103, 2645, 2692
XcmI CCANNNNNNNNNTGG 1 cut(s) 3185
XmiI GTMKAC 4 cut(s) 849, 1455, 1686, 1788
XmnI GAANNNNTTC 5 cut(s) 972, 1275, 2247, 3263, 3525
XspI CTAG 8 cut(s) 399, 1005, 1436, 1451, 1955, 2156, 2444, 3360
ZrmI AGTACT 1 cut(s) 1392
Zsp2I ATGCAT 1 cut(s) 1488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.