pycom17g27530

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000654
Physical Location & Seq
Reverse (-)
25663538 .. 25667839
4302 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g27530.4

Sequence Viewer

Length: 3666 bp
ATGGCTGCAATAATTGCTCAGCTCGATGCCTCTTCCACCCCCTACCGGTGTACTTATGATGTGTTCTTGAGTTTCAGAGGCGAAGATACACGTAAGGGGTTTACTGATCACCTCTACGGGGAGTTGGAGCTGGCAGGAATTCACACCTTTAGAGATGATGATGGGATCGAGAGAGGAGCAAACATAGCATCAGAGCTACAGAAAGCGATACAAGAATCACGAGCATCCATAATTGTCTTCTCCAAGGACTATGCTTCCTCAAGATGGTGCTTGGATGAGCTGGTGAAGATCATGAAACGTAGAAAAACTGATTGCAGACACATGGTCATGCCAGTTTTCTATGATGTGGATCCATCCCATGTTAGGAAACAGACTGGAAGTTTTGCACAAGCATTTGCTAGACATGAAGAACGCTTCAAGGAGGAGATGGACAAGGTGGTGGAGTGGAGAAGAGCCCTTAGAGATGTAGCAGATGTGGGAGGAATGGTTTTAGGACATCTGTATGAGTCACAGTTTATCCAAAATATTGTCGAAGAGATTGGAAATAAACTGGATCATCGCACAACAGAAATGATTGCTCCCTATGTGGTTGGAATAGATAAGCGTGTGCAGGGCATAAACATGTGGCTGAAAGATGGATCAAATGACGTGAGTGTAGCTGTGATCTATGGGATGGGTGGAATTGGCAAGACCACCATTGCCAAAGCTGCTTATAACCAGAACTTCCACAGATTTCAAGGTTGCAGCTATCTTGCAGATGTTAGAGCAACTTCAGAACAGCCTAATGGTTTGGCTTTTTTGCAAAGAAAACTTCTTTCGGATATCCAAAAGGGGAAAAAGAAGAAAATATACAGTATGGGTGAACGAATGAGCAAGATCAAGCATGCTGTGTGTTGCAAAACTGTTCTTATTGTTCTTGATGATGTGAACCAATCCGACCAATTCAATGCAATTCTTGGAAGGCGAGAATGGTTTCACCCAGGAAGTAAAATTATTGTAACAACTAGACATGAAAGTTTGTTGAATGATCATGAAGTTTATGCAATGTTTAAGGTTAGAGGACTGGGTGAGTATGAATCACTTGAGCTTTTTAGTTGGCATGCCTTCAAACAAGTGCGCCCCGTCAAAGATTACATCAAGCTAACAAGATCGGTAGTACAGCACTGTCAAGGGTTACCATTAGCACTTCAAGTTTTGGGATCTTCTCTTGTTGGAAAAAGTGTAGAAGTATGGGAAAGTGCACTGCAGAAGTTAAACGTCATTCCTAATGAAAAAATTCAAAAAATACTCAGAATAAGCTTTGATTCTCTACAAGATGATCATGACAGAAGTTTATTCCTTCATATAGTCTCTTTCTTCGTGGGAAAGAAGATGGATTATACAATTACAGTACTGGACAATTTACGCTTTTACACAAGGATTGGAATTCAAAATCTAGTTGATAGATGTCTAGTAGACATTGATGAAGACAACAAGTTGGTCATGCATCAATTACTTATAGACATGGGAAGGGGAATTATTCGTGAAGAGTCGCCCGAGGACCCTGGGAAACGTAGTAGAGTGCTACAGAATGATGCCTCTGATATTTTGAGAAAATTAACTGGAACAAAAAATATTAAGGGCCTCATGCTCAACCTTCCTGGCAAGACAATGTTTAATACAAGTAACAAAAAGAGATACCATGTCGACGATTACCATGAAAAAGTCTCAAGGCGACGGCTTGGTTTCTTCTCTTGGAAATCTATTACCTTTCTGCCAACAAATTCTGCTTCTGAATCAAATGAGGTAGACTTCAAAACAGAGGCATTTGCAAGGATGCACAATCTTGAACTTCTGTTGCTCAATAATGTAAACGTCAGTGGAGGCTACAAAGATTTTCCAAAAAATTTAAGATGGATATCTTGGCGAGTATTCCCTTTAAAATCAATACCAGCAGAGTTATATTTGGAAAATCTAGTGGGTCTTGACTTGCGGAATAGCAGGCTACATCATCTTTGGCAAGGACCCAGGGCTATTCCAAGACTGAAATTCCTTAATGTCAGTCATTCACATGGTCTTGTGACAACCCCCAACTTTTCAGGAGTCCCTAACATTGAGAAATTGGTTCTTAAAGATTGCACAAATTTGGTTTTGATTGATGAATCCATTGCAGACCTAGAGAAACTAATTGTGTTGAATCTAAAAGACTGCAAAAATCTCACAAAGCTTCCAACAAGATTTAGCATGTTGACATCTCTTCAGGAACTGATTCTTTCTGGTTGCTCAAAGCTTGTGTTTCGCCCCAATGCTGCAGCTGGTGTTTGGAAGAAACTCAATATATCAACAGCTACGCTATCGCAATCAATGTGGTTGTGGAGACTCTGGATATTAGCCAGGAAAAATCTTGTTACAGCCCGCAGTTTGTTAGTGGCAAATTGGCCAAATGGCTTAGTAAGCTTAAGTCTAGCTGACTGCAATCTTCCAGAGATTCCCAGTGGTCTTGGTATCCTATCTTCATTGAAGCATTTGAATCTAAGTAGAAACCCAATTTTGAGCCTACCAGAAAACCTGAATGATCTTATTACGCGCCAAACTCTTGTAATTGAAGGTTGCACAATGGTCCGAACTCTTCCAGAACTCCCACCAAGTTTAACAAAATTGCATGCAAGTTATTGTACCTCATTGGAAAAAATAACAAATTTACCAAACATGTTCAGATCATTGGATTCAAGTTTTTGGAAGTGCAAGAATTTAGTTGAGGTTCAAAGCTTGTTCCATATAAAACCATTGAGAAGGGTTGACATTGAAATGATCAGAGATATGGACCTGTTCAATTTGGAATCCGTAGGAAGCACTGAAGTTGAGATGACCAATTACTTGACATGTACAACAAGAAAGGGTCCTATACAGGGACTGTATGAATGTGGCATATTTAGCGTTTTCCTTCACGGAAACAAGATTCCAGATAGGTTCCCATACAGAAGCCTGCGCAACTCCACTTTTTCTATTATTGTACCTTCACATCTTAATCTCAAGGTCCGAGGCTTGAATGCTTGTGTTGTATATTCGCGATGTCCATTTTGGTTTAGCAGTTCAAACTTCCTTAAAGTCAGTAATGAGACCAAGGGTCTTAAATGGACATATTGCCCAGTCACAGCAGGTCTCCCGAGAAAGAACCATGATATGTTATGGCTAAGCCATTGGTTGTTTGGAAACGATGAACTGGAGGGCGGGGATGAAGTACATTTTTCCATGAATGAAGAATATAGTTTCTGGACGAAGGAGTTCGGTATCCAGCTTGTGTATGAGCAGGAAAATGAGGGTAAGGGTGTTCAATCAAAAAGTGAAGATATGACATTCCAACCAAATACAACTCTTTCTAGCCCGGTTTTTGTTGCTGGAAATGTATCTGCGTCAGCATCGAAGTATCAGTTATGGACGGGCAAATTCTTTCTTTGCAATCATCGCAGTCGTATCCATCAACTTCATTTCTCTAGGACCCCGGAGAAGCCAGATTATCTTAATTTTTCATACTTATTTAAGGAAGATGTTCCTGCTAAGACGATGGTTCCATCTACTGCAATGAATGTTACACCAATTCCTTTTCAGTGGACATCCCCTGAGTTTTTACTTCATCCAGGATGTGTGATGTATATGCCATTCCGAAGTGATTTATTAATCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1222

Amino Acids

139.59

Weight (kDa)

8.99

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
Acc16I TGCGCA 1 cut(s) 2969
Acc36I ACCTGC 1 cut(s) 3128
AccI GTMKAC 3 cut(s) 1455, 1686, 1788
AccII CGCG 2 cut(s) 2566, 3049
AciI CCGC 3 cut(s) 1972, 2395, 3210
AclWI GGATC 6 cut(s) 173, 344, 357, 561, 646, 1207
AcoI YGGCCR 1 cut(s) 2417
AcuI CTGAAG 3 cut(s) 756, 2222, 2856
AfaI GTAC 7 cut(s) 52, 1158, 1392, 2656, 2866, 2994, 3222
AfiI CCNNNNNNNGG 5 cut(s) 45, 118, 264, 363, 2888
AflII CTTAAG 1 cut(s) 2437
AflIII ACRYGT 4 cut(s) 89, 621, 2688, 2861
AgeI ACCGGT 1 cut(s) 45
AhdI GACNNNNNGTC 2 cut(s) 323, 3105
AjiI CACGTC 1 cut(s) 649
AjnI CCWGG 6 cut(s) 979, 1543, 1639, 2006, 2372, 3616
Alw21I GWGCWC 1 cut(s) 1243
Alw26I GTCTC 5 cut(s) 1354, 1711, 2350, 3092, 3146
Alw44I GTGCAC 1 cut(s) 1239
AlwI GGATC 6 cut(s) 173, 344, 357, 561, 646, 1207
AlwNI CAGNNNCTG 2 cut(s) 2245, 2893
Ama87I CYCGRG 2 cut(s) 1535, 3145
AoxI GGCC 2 cut(s) 1621, 2417
ApaLI GTGCAC 1 cut(s) 1239
ApeKI GCWGC 5 cut(s) 5, 707, 744, 2288, 2291
AseI ATTAAT 1 cut(s) 3656
AsiGI ACCGGT 1 cut(s) 45
Asp700I GAANNNNTTC 5 cut(s) 972, 1275, 2247, 3263, 3528
AspLEI GCGC 3 cut(s) 1119, 2568, 2970
AspS9I GGNCC 8 cut(s) 1540, 1621, 2003, 2599, 2803, 2879, 3016, 3477
AsuC2I CCSGG 2 cut(s) 3365, 3482
AsuHPI GGTGA 5 cut(s) 101, 295, 872, 968, 1079
AvaI CYCGRG 2 cut(s) 1535, 3145
AvaII GGWCC 7 cut(s) 1540, 2003, 2599, 2803, 2879, 3016, 3477
BaeGI GKGCMC 1 cut(s) 1243
BalI TGGCCA 1 cut(s) 2419
BamHI GGATCC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 457
BarI GAAGNNNNNNTAC 4 cut(s) 833, 865, 2476, 2508
BauI CACGAG 1 cut(s) 219
BbsI GAAGAC 2 cut(s) 229, 1473
Bbv12I GWGCWC 1 cut(s) 1243
BbvI GCAGC 4 cut(s) 694, 756, 2275, 2303
BceAI ACGGC 1 cut(s) 1733
BciT130I CCWGG 6 cut(s) 981, 1545, 1641, 2008, 2374, 3618
BciVI GTATCC 3 cut(s) 2495, 3281, 3464
BclI TGATCA 4 cut(s) 106, 1027, 1318, 2790
BcnI CCSGG 2 cut(s) 3365, 3482
BcoDI GTCTC 5 cut(s) 1354, 1711, 2350, 3092, 3146
BfaI CTAG 9 cut(s) 399, 1005, 1436, 1451, 1955, 2156, 2444, 3360, 3474
BfmI CTRYAG 4 cut(s) 197, 1244, 1565, 2289
BfrI CTTAAG 1 cut(s) 2437
BfuAI ACCTGC 1 cut(s) 3128
BfuI GTATCC 3 cut(s) 2495, 3281, 3464
BisI GCNGC 5 cut(s) 6, 708, 745, 2289, 2292
BlpI GCTNAGC 2 cut(s) 18, 3173
BlsI GCNGC 5 cut(s) 7, 709, 746, 2290, 2293
BmcAI AGTACT 1 cut(s) 1392
Bme1390I CCNGG 8 cut(s) 981, 1545, 1641, 2008, 2374, 3365, 3482, 3618
Bme18I GGWCC 7 cut(s) 1540, 2003, 2599, 2803, 2879, 3016, 3477
BmeRI GACNNNNNGTC 2 cut(s) 323, 3105
BmeT110I CYCGRG 2 cut(s) 1535, 3145
BmgBI CACGTC 1 cut(s) 649
BmgT120I GGNCC 8 cut(s) 1540, 1621, 2003, 2599, 2803, 2879, 3016, 3477
BmiI GGNNCC 7 cut(s) 351, 1542, 2005, 2880, 2951, 3479, 3549
BmrFI CCNGG 8 cut(s) 981, 1545, 1641, 2008, 2374, 3365, 3482, 3618
BmrI ACTGGG 3 cut(s) 1073, 2466, 3122
BmsI GCATC 7 cut(s) 16, 197, 233, 1495, 1564, 1806, 3407
BmuI ACTGGG 3 cut(s) 1073, 2466, 3122
BpiI GAAGAC 2 cut(s) 229, 1473
BpmI CTGGAG 1 cut(s) 3224
Bpu1102I GCTNAGC 2 cut(s) 18, 3173
BpuEI CTTGAG 5 cut(s) 88, 244, 1103, 1693, 2996
BpuMI CCSGG 2 cut(s) 3365, 3482
BsaAI YACGTR 1 cut(s) 92
BsaBI GATNNNNATC 2 cut(s) 348, 1897
BsaI GGTCTC 2 cut(s) 3092, 3146
BsaWI WCCGGW 1 cut(s) 45
BsaXI ACNNNNNCTCC 2 cut(s) 471, 501
Bsc4I CCNNNNNNNGG 5 cut(s) 45, 118, 264, 363, 2888
Bse118I RCCGGY 1 cut(s) 45
Bse1I ACTGG 9 cut(s) 332, 379, 555, 1068, 1398, 1606, 2472, 3128, 3207
Bse3DI GCAATG 4 cut(s) 696, 1050, 2145, 3567
Bse8I GATNNNNATC 2 cut(s) 348, 1897
BseBI CCWGG 6 cut(s) 981, 1545, 1641, 2008, 2374, 3618
BseGI GGATG 9 cut(s) 224, 280, 353, 678, 1821, 3220, 3593, 3613, 3626
BseJI GATNNNNATC 2 cut(s) 348, 1897
BseLI CCNNNNNNNGG 5 cut(s) 45, 118, 264, 363, 2888
BseMI GCAATG 4 cut(s) 696, 1050, 2145, 3567
BseMII CTCAG 3 cut(s) 32, 1303, 3591
BseNI ACTGG 9 cut(s) 332, 379, 555, 1068, 1398, 1606, 2472, 3128, 3207
BseRI GAGGAG 2 cut(s) 189, 437
BseSI GKGCMC 1 cut(s) 1243
BseXI GCAGC 4 cut(s) 694, 756, 2275, 2303
BsgI GTGCAG 1 cut(s) 629
Bsh1236I CGCG 2 cut(s) 2566, 3049
BshFI GGCC 2 cut(s) 1623, 2419
BshTI ACCGGT 1 cut(s) 45
BsiHKAI GWGCWC 1 cut(s) 1243
BsiHKCI CYCGRG 2 cut(s) 1535, 3145
BsiSI CCGG 3 cut(s) 46, 3365, 3482
BslFI GGGAC 2 cut(s) 2069, 2904
BslI CCNNNNNNNGG 5 cut(s) 45, 118, 264, 363, 2888
BsmAI GTCTC 5 cut(s) 1354, 1711, 2350, 3092, 3146
BsmFI GGGAC 2 cut(s) 2069, 2904
BsmI GAATGC 1 cut(s) 3034
BsnI GGCC 2 cut(s) 1623, 2419
Bso31I GGTCTC 2 cut(s) 3092, 3146
BsoBI CYCGRG 2 cut(s) 1535, 3145
Bsp1286I GDGCHC 2 cut(s) 457, 1243
Bsp1407I TGTACA 1 cut(s) 2864
Bsp1720I GCTNAGC 2 cut(s) 18, 3173
Bsp68I TCGCGA 1 cut(s) 3049
BspACI CCGC 3 cut(s) 1972, 2395, 3210
BspANI GGCC 2 cut(s) 1623, 2419
BspCNI CTCAG 3 cut(s) 31, 1302, 3592
BspFNI CGCG 2 cut(s) 2566, 3049
BspHI TCATGA 3 cut(s) 291, 1030, 1321
BspLI GGNNCC 7 cut(s) 351, 1542, 2005, 2880, 2951, 3479, 3549
BspMAI CTGCAG 2 cut(s) 1248, 2293
BspMI ACCTGC 1 cut(s) 3128
BspPI GGATC 6 cut(s) 173, 344, 357, 561, 646, 1207
BspQI GCTCTTC 1 cut(s) 445
BspTI CTTAAG 1 cut(s) 2437
BspTNI GGTCTC 2 cut(s) 3092, 3146
BsrDI GCAATG 4 cut(s) 696, 1050, 2145, 3567
BsrFI RCCGGY 1 cut(s) 45
BsrGI TGTACA 1 cut(s) 2864
BsrI ACTGG 9 cut(s) 332, 379, 555, 1068, 1398, 1606, 2472, 3128, 3207
BssAI RCCGGY 1 cut(s) 45
BssSI CACGAG 1 cut(s) 219
BssT1I CCWWGG 2 cut(s) 243, 3102
Bst2BI CACGAG 1 cut(s) 219
Bst2UI CCWGG 6 cut(s) 981, 1545, 1641, 2008, 2374, 3618
Bst4CI ACNGT 6 cut(s) 513, 854, 904, 1166, 1390, 2895
Bst6I CTCTTC 6 cut(s) 37, 445, 528, 1521, 2241, 2613
BstAFI CTTAAG 1 cut(s) 2437
BstAPI GCANNNNNTGC 1 cut(s) 14
BstAUI TGTACA 1 cut(s) 2864
BstBAI YACGTR 1 cut(s) 92
BstC8I GCNNGC 7 cut(s) 132, 885, 1101, 1982, 2395, 2643, 2966
BstDEI CTNAG 8 cut(s) 18, 458, 1289, 2428, 2513, 3173, 3537, 3600
BstEII GGTNACC 1 cut(s) 1173
BstF5I GGATG 9 cut(s) 224, 280, 353, 678, 1821, 3220, 3593, 3613, 3626
BstFNI CGCG 2 cut(s) 2566, 3049
BstHHI GCGC 3 cut(s) 1119, 2568, 2970
BstMAI GTCTC 5 cut(s) 1354, 1711, 2350, 3092, 3146
BstMWI GCNNNNNNNGC 6 cut(s) 14, 185, 707, 2433, 2913, 3444
BstNI CCWGG 6 cut(s) 981, 1545, 1641, 2008, 2374, 3618
BstNSI RCATGY 7 cut(s) 625, 887, 1103, 2227, 2645, 2692, 2865
BstPI GGTNACC 1 cut(s) 1173
BstSCI CCNGG 8 cut(s) 979, 1543, 1639, 2006, 2372, 3363, 3480, 3616
BstSFI CTRYAG 4 cut(s) 197, 1244, 1565, 2289
BstSLI GKGCMC 1 cut(s) 1243
BstUI CGCG 2 cut(s) 2566, 3049
BstV1I GCAGC 4 cut(s) 694, 756, 2275, 2303
BstV2I GAAGAC 2 cut(s) 229, 1473
BstX2I RGATCY 2 cut(s) 349, 1199
BstYI RGATCY 2 cut(s) 349, 1199
BsuI GTATCC 3 cut(s) 2495, 3281, 3464
BsuRI GGCC 2 cut(s) 1623, 2419
BtgZI GCGATG 3 cut(s) 542, 3064, 3428
BtrI CACGTC 1 cut(s) 649
BtsCI GGATG 9 cut(s) 224, 280, 353, 678, 1821, 3220, 3593, 3613, 3626
BtsI GCAGTG 1 cut(s) 1241
BtsIMutI CAGTG 6 cut(s) 1162, 1241, 1864, 2479, 2832, 3593
BtuMI TCGCGA 1 cut(s) 3049
BveI ACCTGC 1 cut(s) 3128
Cac8I GCNNGC 7 cut(s) 132, 885, 1101, 1982, 2395, 2643, 2966
CaiI CAGNNNCTG 2 cut(s) 2245, 2893
CciI TCATGA 3 cut(s) 291, 1030, 1321
CfoI GCGC 3 cut(s) 1119, 2568, 2970
Cfr10I RCCGGY 1 cut(s) 45
Cfr13I GGNCC 8 cut(s) 1540, 1621, 2003, 2599, 2803, 2879, 3016, 3477
CseI GACGC 1 cut(s) 3381
Csp6I GTAC 7 cut(s) 51, 1157, 1391, 2655, 2865, 2993, 3221
CspAI ACCGGT 1 cut(s) 45
CspCI CAANNNNNGTGG 2 cut(s) 2327, 2362
CviQI GTAC 7 cut(s) 51, 1157, 1391, 2655, 2865, 2993, 3221
DdeI CTNAG 8 cut(s) 18, 458, 1289, 2428, 2513, 3173, 3537, 3600
DraI TTTAAA 1 cut(s) 1920
DriI GACNNNNNGTC 2 cut(s) 323, 3105
EaeI YGGCCR 1 cut(s) 2417
Eam1104I CTCTTC 6 cut(s) 37, 445, 528, 1521, 2241, 2613
Eam1105I GACNNNNNGTC 2 cut(s) 323, 3105
EarI CTCTTC 6 cut(s) 37, 445, 528, 1521, 2241, 2613
Eco130I CCWWGG 2 cut(s) 243, 3102
Eco24I GRGCYC 1 cut(s) 457
Eco31I GGTCTC 2 cut(s) 3092, 3146
Eco32I GATATC 2 cut(s) 823, 1899
Eco47I GGWCC 7 cut(s) 1540, 2003, 2599, 2803, 2879, 3016, 3477
Eco57I CTGAAG 3 cut(s) 756, 2222, 2856
Eco88I CYCGRG 2 cut(s) 1535, 3145
Eco91I GGTNACC 1 cut(s) 1173
EcoO109I RGGNCCY 5 cut(s) 1540, 1621, 2003, 2879, 3477
EcoO65I GGTNACC 1 cut(s) 1173
EcoRI GAATTC 2 cut(s) 138, 1425
EcoRII CCWGG 6 cut(s) 979, 1543, 1639, 2006, 2372, 3616
EcoRV GATATC 2 cut(s) 823, 1899
EcoT14I CCWWGG 2 cut(s) 243, 3102
EcoT22I ATGCAT 1 cut(s) 1488
EcoT38I GRGCYC 1 cut(s) 457
ErhI CCWWGG 2 cut(s) 243, 3102
FaqI GGGAC 2 cut(s) 2069, 2904
FauI CCCGC 2 cut(s) 2402, 3203
FbaI TGATCA 4 cut(s) 106, 1027, 1318, 2790
FblI GTMKAC 3 cut(s) 1455, 1686, 1788
Fnu4HI GCNGC 5 cut(s) 6, 708, 745, 2289, 2292
FokI GGATG 9 cut(s) 211, 287, 340, 685, 1828, 3227, 3580, 3600, 3633
FriOI GRGCYC 1 cut(s) 457
Fsp4HI GCNGC 5 cut(s) 6, 708, 745, 2289, 2292
FspBI CTAG 9 cut(s) 399, 1005, 1436, 1451, 1955, 2156, 2444, 3360, 3474
FspI TGCGCA 1 cut(s) 2969
GlaI GCGC 3 cut(s) 1118, 2567, 2969
GluI GCNGC 5 cut(s) 6, 708, 745, 2289, 2292
GsuI CTGGAG 1 cut(s) 3224
HaeIII GGCC 2 cut(s) 1623, 2419
HapII CCGG 3 cut(s) 46, 3365, 3482
HgaI GACGC 1 cut(s) 3381
HhaI GCGC 3 cut(s) 1119, 2568, 2970
Hin6I GCGC 3 cut(s) 1117, 2566, 2968
HinP1I GCGC 3 cut(s) 1117, 2566, 2968
HincII GTYRAC 3 cut(s) 1687, 2229, 2779
HindII GTYRAC 3 cut(s) 1687, 2229, 2779
HindIII AAGCTT 5 cut(s) 1297, 2204, 2267, 2434, 2746
HpaII CCGG 3 cut(s) 46, 3365, 3482
HphI GGTGA 5 cut(s) 101, 295, 872, 968, 1079
Hpy99I CGWCG 2 cut(s) 1691, 1719
HpyCH4III ACNGT 6 cut(s) 513, 854, 904, 1166, 1390, 2895
HpyCH4IV ACGT 6 cut(s) 91, 298, 648, 1257, 1552, 1854
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 185, 707, 2433, 2913, 3444
HpyF3I CTNAG 8 cut(s) 18, 458, 1289, 2428, 2513, 3173, 3537, 3600
HpySE526I ACGT 6 cut(s) 91, 298, 648, 1257, 1552, 1854
HspAI GCGC 3 cut(s) 1117, 2566, 2968
Ksp22I TGATCA 4 cut(s) 106, 1027, 1318, 2790
LguI GCTCTTC 1 cut(s) 445
LmnI GCTCC 3 cut(s) 127, 176, 583
Lsp1109I GCAGC 4 cut(s) 694, 756, 2275, 2303
LweI GCATC 7 cut(s) 16, 197, 233, 1495, 1564, 1806, 3407
MaeI CTAG 9 cut(s) 399, 1005, 1436, 1451, 1955, 2156, 2444, 3360, 3474
MaeII ACGT 6 cut(s) 91, 298, 648, 1257, 1552, 1854
MaeIII GTNAC 8 cut(s) 507, 997, 1173, 1664, 2059, 2386, 3130, 3568
MflI RGATCY 2 cut(s) 349, 1199
MhlI GDGCHC 2 cut(s) 457, 1243
MlsI TGGCCA 1 cut(s) 2419
MluNI TGGCCA 1 cut(s) 2419
MlyI GAGTC 4 cut(s) 515, 1538, 2091, 2352
MmeI TCCRAC 6 cut(s) 105, 571, 960, 1192, 2234, 3364
Mox20I TGGCCA 1 cut(s) 2419
Mph1103I ATGCAT 1 cut(s) 1488
MroXI GAANNNNTTC 5 cut(s) 972, 1275, 2247, 3263, 3528
MscI TGGCCA 1 cut(s) 2419
MslI CAYNNNNRTG 5 cut(s) 326, 501, 620, 2049, 2786
Msp20I TGGCCA 1 cut(s) 2419
MspA1I CMGCKG 1 cut(s) 2294
MspCI CTTAAG 1 cut(s) 2437
MspI CCGG 3 cut(s) 46, 3365, 3482
MspR9I CCNGG 8 cut(s) 981, 1545, 1641, 2008, 2374, 3365, 3482, 3618
Mva1269I GAATGC 1 cut(s) 3034
MvaI CCWGG 6 cut(s) 981, 1545, 1641, 2008, 2374, 3618
MvnI CGCG 2 cut(s) 2566, 3049
MwoI GCNNNNNNNGC 6 cut(s) 14, 185, 707, 2433, 2913, 3444
NciI CCSGG 2 cut(s) 3365, 3482
NlaIV GGNNCC 7 cut(s) 351, 1542, 2005, 2880, 2951, 3479, 3549
NmuCI GTSAC 3 cut(s) 507, 2059, 3130
NruI TCGCGA 1 cut(s) 3049
NsbI TGCGCA 1 cut(s) 2969
NsiI ATGCAT 1 cut(s) 1488
NspI RCATGY 7 cut(s) 625, 887, 1103, 2227, 2645, 2692, 2865
PaeI GCATGC 3 cut(s) 887, 1103, 2645
PagI TCATGA 3 cut(s) 291, 1030, 1321
PasI CCCWGGG 2 cut(s) 1544, 2007
PciI ACATGT 3 cut(s) 621, 2688, 2861
PciSI GCTCTTC 1 cut(s) 445
PctI GAATGC 1 cut(s) 3034
PdmI GAANNNNTTC 5 cut(s) 972, 1275, 2247, 3263, 3528
PfoI TCCNGGA 1 cut(s) 3616
PinAI ACCGGT 1 cut(s) 45
PkrI GCNGC 5 cut(s) 7, 709, 746, 2290, 2293
PleI GAGTC 4 cut(s) 514, 1537, 2090, 2352
PpsI GAGTC 4 cut(s) 514, 1537, 2090, 2352
Ppu21I YACGTR 1 cut(s) 92
PpuMI RGGWCCY 4 cut(s) 1540, 2003, 2879, 3477
PscI ACATGT 3 cut(s) 621, 2688, 2861
PshBI ATTAAT 1 cut(s) 3656
PsiI TTATAA 1 cut(s) 714
Psp5II RGGWCCY 4 cut(s) 1540, 2003, 2879, 3477
Psp6I CCWGG 6 cut(s) 979, 1543, 1639, 2006, 2372, 3616
PspEI GGTNACC 1 cut(s) 1173
PspGI CCWGG 6 cut(s) 979, 1543, 1639, 2006, 2372, 3616
PspN4I GGNNCC 7 cut(s) 351, 1542, 2005, 2880, 2951, 3479, 3549
PspPI GGNCC 8 cut(s) 1540, 1621, 2003, 2599, 2803, 2879, 3016, 3477
PspPPI RGGWCCY 4 cut(s) 1540, 2003, 2879, 3477
PstI CTGCAG 2 cut(s) 1248, 2293
PstNI CAGNNNCTG 2 cut(s) 2245, 2893
PsuI RGATCY 2 cut(s) 349, 1199
PvuII CAGCTG 1 cut(s) 2294
RruI TCGCGA 1 cut(s) 3049
RsaI GTAC 7 cut(s) 52, 1158, 1392, 2656, 2866, 2994, 3222
RsaNI GTAC 7 cut(s) 51, 1157, 1391, 2655, 2865, 2993, 3221
RseI CAYNNNNRTG 5 cut(s) 326, 501, 620, 2049, 2786
SalI GTCGAC 1 cut(s) 1685
SapI GCTCTTC 1 cut(s) 445
SatI GCNGC 5 cut(s) 6, 708, 745, 2289, 2292
Sau96I GGNCC 8 cut(s) 1540, 1621, 2003, 2599, 2803, 2879, 3016, 3477
ScaI AGTACT 1 cut(s) 1392
SchI GAGTC 4 cut(s) 515, 1538, 2091, 2352
ScrFI CCNGG 8 cut(s) 981, 1545, 1641, 2008, 2374, 3365, 3482, 3618
SduI GDGCHC 2 cut(s) 457, 1243
SfaNI GCATC 7 cut(s) 16, 197, 233, 1495, 1564, 1806, 3407
SfcI CTRYAG 4 cut(s) 197, 1244, 1565, 2289
SinI GGWCC 7 cut(s) 1540, 2003, 2599, 2803, 2879, 3016, 3477
SmiMI CAYNNNNRTG 5 cut(s) 326, 501, 620, 2049, 2786
SmlI CTYRAG 6 cut(s) 67, 259, 1082, 1708, 2437, 3011
SmoI CTYRAG 6 cut(s) 67, 259, 1082, 1708, 2437, 3011
SphI GCATGC 3 cut(s) 887, 1103, 2645
SsiI CCGC 3 cut(s) 1972, 2395, 3210
SspI AATATT 2 cut(s) 526, 1615
SspMI CTAG 9 cut(s) 399, 1005, 1436, 1451, 1955, 2156, 2444, 3360, 3474
StyD4I CCNGG 8 cut(s) 979, 1543, 1639, 2006, 2372, 3363, 3480, 3616
StyI CCWWGG 2 cut(s) 243, 3102
TaaI ACNGT 6 cut(s) 513, 854, 904, 1166, 1390, 2895
TaiI ACGT 6 cut(s) 94, 301, 651, 1260, 1555, 1857
TaqI TCGA 5 cut(s) 24, 168, 531, 1686, 3401
TatI WGTACW 5 cut(s) 50, 1156, 1390, 2864, 3220
TscAI CASTG 6 cut(s) 1169, 1248, 1864, 2479, 2839, 3593
TseFI GTSAC 3 cut(s) 507, 2059, 3130
TseI GCWGC 5 cut(s) 5, 707, 744, 2288, 2291
Tsp45I GTSAC 3 cut(s) 507, 2059, 3130
TspGWI ACGGA 2 cut(s) 2812, 2943
TspRI CASTG 6 cut(s) 1169, 1248, 1864, 2479, 2839, 3593
Vha464I CTTAAG 1 cut(s) 2437
VneI GTGCAC 1 cut(s) 1239
VpaK11BI GGWCC 7 cut(s) 1540, 2003, 2599, 2803, 2879, 3016, 3477
VspI ATTAAT 1 cut(s) 3656
XceI RCATGY 7 cut(s) 625, 887, 1103, 2227, 2645, 2692, 2865
XcmI CCANNNNNNNNNTGG 1 cut(s) 3185
XmiI GTMKAC 3 cut(s) 1455, 1686, 1788
XmnI GAANNNNTTC 5 cut(s) 972, 1275, 2247, 3263, 3528
XspI CTAG 9 cut(s) 399, 1005, 1436, 1451, 1955, 2156, 2444, 3360, 3474
ZrmI AGTACT 1 cut(s) 1392
Zsp2I ATGCAT 1 cut(s) 1488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.