Rw1G013150

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000654
Physical Location & Seq
Reverse (-)
29374203 .. 29378776
4574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G013150.1

Sequence Viewer

Length: 3594 bp
ATGGCTGCTTCTCCATCCAGATGCCGCTATCATGCCTTCCTGAGTTTCAGGGGCGAAGATACACGCAAGGGCTTTAGTGATCATCTCTATAGAGCTTTGGAGTTGGCTGGAATTCACACCTTAAGAGATGATGAAGAAATTGAGGGAGGAGCAGAGATAGCAGTAGAGCTGCAGAAGGCTATCCAGGAATCACAGGTATCAATAATCGTGTTTTCCAAGAATTATGCTTCCTCAAGATGGTGCTTGGATGAGCTTGCCAATATCATGAAACGTAGAAGAAAAGATGGACACATGGTTTTGCCAGTTTTCTATAAGGTGGATCCATCCAATGTTAGACATCAGACTGGCAGTTTTGAAGAAGCGTTTGCCAAACATGAAGAGCGCTTCAAGGAGGAGATGGGCAAGGTGCACGAGTGGAGAAGATCTCTTAAAGATGCTGCCGATCTGGCTGATCCAAATATTGTTGAGAAGATTGGAAATAAACTGGAACCAAAACTATTAAATCTACCTCCCTATGCGGTTGGAATAGATGATCGTGTGGAAGATATAAACATGTGGTTAAGAGAAGGATCAACTGATGTTGGTGTAGCTGGGATTTATGGAATGGGTGGAATTGGCAAGACCACCATTGCAAAAGCTGCTTATAACATGAACTTGGGTAACTTTCAAGGAAGTAGCTTTCTTGCAGATATTAGAGCAACTTCAGAACAACCTAATGGTTTGGTTCGCTTGCAAATGAAACTTATATCTGATATCCAGAAGCAGAAGCCAAAGAAGATATACTCTGTTAATGAAGGAATGAGCCGGATCCAGCACGTTGTATGTTGCAAAAGAGTTCTTATTGTTCTTGATGATGTAGATGATTTGGACCAATTCAGGGCAATTCTTGGAATGAGAGAATGGTTTCATCCGGGAAGTAAGATTATCATAACAACTAGACATTTACACTTGCTGAAAGCCAATGCGGTTTCTACAAGGTTCATGGTACAAGAACTTGATGAGCATGAATCACTTGAGCTTTTCTGTTGGCATGCTTTTCGACAAGCTCATCCTATCAAAAGCTACATGGAGGTTTCTACAGATGTAGTACAACATTGTGGAGGGCTTCCGTTAGCTATTCGAGTTTTGGGGTCTTCTCTGTATGGAGAAAGTGTAGATGTATGGCAAAGTGCATTAGAGAAGTTGGATGTGATTCCTCATCGCGATATCCAAAAGGTTCTAAGAGTAAGCTTTGATTCTCTGCAAGATGATTACGACAAAAATTTGTTTCTTCATATATCCTGTTTCTTCATTGGAAAGGAGAAAAAATTTGCAATTACAGTACTGAACGACCTTGATCTTTTCCCAATAATTGGAATTAAAAATCTAGTCGATAGATGTTTATTGCAAATTGATAATGACAGGTTGATCATGAATCAATTACTTATTGACATGGCAAGGGGAATTATTCGTGAAGAGTCCCCAGAGAACCCTGGAAAACGTAGTAGACTGTGGGATGAGGATGCATTTGATGTTTTGGATAACATAACAGTAAGACACATCTGCGATCTTCAAGTGTATGCTTATGTAGTTGCGTTGTTCATTTACACTAATTACTTAACCAACTGTTCTGATTTGTTGGAAAATACCCTTTTTAAAAATAAACGATGCCACACTGAAGAGTACGATAGAAATTGTTCCAGATGGCGTTGGTTTGGTTTCTTCTCATGGAAATTAAACAACTTTTCCTCAACAGGGTTATATTCAGATGTGCCAAACTTCAAATCAGAGGCACTTAAAAGGATGCACGATCTTAAAATTCTTCTGCTCAATTTTGTACAATTCAGTGGTTGCTGTGACAACTTTCCAAAAAAAATATCATGGTTTTCTTGGCAAGGGTTTCCTTCAGAATCTATACCGAAAGAATTTCATTTGCAAACTCTAGTTGTTTTGGAGTTGCAAAACAGCCAACTGAAAGTTGTTTGGGAGGGGAAAAAGAATCTGCCGAAATTAAAAATCCTTGATCTCAGTGATTCACTTGATCTTGTTACAACCCCCGACTTGTCAGGACTCTCTAACCTTGAGAGATTAATCCTTGAAGGTTGCACGAACATTGCTGAGATTGATGAATCCATTGGTGATCTTGAGAAACTTGTTTCCTTGAATCTCAAATACTGCAAAAATCTCGAGAAGCTCCCGGAGACAATTGACAGGTTGACATCTCTTCAGGAGCTTAACCTTTCTGGTTGTTCAAACCTTGCTCTTCATGCTAATACAGCTACTAATATGATGAATTTCTTCAGCCTGTTATCAAATAAATCCTGGAAATCAATTTGGTCGCGTGTATCACCAAGAAAAAGTGTTGAACCAACCAGTTTGTCATTGGTAAATTTGCCACATTCTTTAGTACGGTTGAGACTGCATCGTTGCAGTATATCAGAGATTCCCAATGATCTTAGTAGCTTTTCCTCATTGAAGGAACTGGATCTAAGCTTTAATCCATTTATGAGTCTACCTGAAAACATGAAGAGTCTTACCGTGCTCGAGCGTCTCCGTATAGACTTCTGCGAAAACGTCAAAATGCTTCCGGAGCTCCCACCAAGTTTATGTGGATTACAAGCAATGAAGTGTACATCATTGAAAAAGGTAACAAATGTACCCGATATGCGGGGTCCACAGTTAGTCAATTTTTTTGCCGCTGGAGAATTAGTTGAAGTTGGAGGCATGTTCAGTATAAAGCCATTGATCAGCGAAATTGACATGGAAATGATAAGAAATATTGGCCTGTACAATTTGGAACCCTCTGGAGGAACTGAGGAGCTTGAAATGATAAATTTCTTGACAGTAACAAGCAGGACATGTCCCCTCCAGGGACTCTGTGAATGTGGCATATTCAGCATTTTCCTTCGTGGAAGCAATATTCCTGATTGGTACGAGTACAGGGGTGAAACCGCGTTGTCCATCACTGTTCCTTCCCATCCTAAACTCAAGATTAAAGGCTTAAATGTCTGTGTTGTGTACACCCCAGATTATGATGTGCACTCCAGAGATGAAGTTGAAGATGAAGATCCGATGATGGGTTCACTCATTCTAAAAGTCAGTAATGAAGCGAAGGGTCTTATGTGGTCATATTACCCTGTCAGTGTAGGTCTTCCAAAAGTGAATGAAGAAATGTCATGGCTAAGCCATTGGCAGTTTGAGAATGATGAGTTAGAGGGCGGGGAAGAACTACGTTTTTCTGTGGATAGCAAATATTTCAGGTTTCCTGTGCACAGAGATGTTCGGCTCTTGATAAAGGAGCTTGCAGTCCAACTTGTGTACGAGAATGAAAATGAGGGCAAGGCATGTAATGGTATCCAATTATCAAATCCTGAAGATGTTACGACCCAGGCTGTCCTCATTGGACACGTATCGGCACCAAAGTATCAAATGCTGGCAGGCAAATACTTCCTCTACAATATTCACGGAGTTGCTCGAAATTTTCTTTCTTCAGTTCAAGAGTACACAGATTTGAATAGTTTATTGGGAAAATCAATTGGGTTCCCTTTTCAGCACGAAGATTTTGAGAGACGTGTCTTGGGACAAAAAAATGAGGAACGAATTTTGCTTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1197

Amino Acids

136.6

Weight (kDa)

5.79

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 10 - 148 8.6e-46 TIR domain
TIR_2 PF13676 13 - 108 1.1e-15 TIR domain
NB-ARC PF00931 177 - 348 5.4e-20 NB-ARC domain
WHD_ROQ1 PF23282 418 - 487 3.6e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 636 - 743 8.5e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 645
AccB1I GGYRCC 1 cut(s) 3394
AccB7I CCANNNNNTGG 1 cut(s) 1352
AccI GTMKAC 2 cut(s) 1486, 2491
AccII CGCG 3 cut(s) 1203, 2320, 2933
AccIII TCCGGA 1 cut(s) 2566
AciI CCGC 7 cut(s) 25, 518, 965, 2647, 2676, 2931, 3198
AclWI GGATC 8 cut(s) 314, 327, 446, 577, 802, 815, 2472, 3041
AcuI CTGAAG 7 cut(s) 687, 1677, 1869, 2189, 2263, 3372, 3453
AfeI AGCGCT 1 cut(s) 383
AfiI CCNNNNNNNGG 8 cut(s) 237, 877, 1352, 1734, 2646, 2786, 2849, 3056
AflII CTTAAG 1 cut(s) 121
AflIII ACRYGT 4 cut(s) 552, 2837, 3385, 3550
AjiI CACGTC 1 cut(s) 3551
AjnI CCWGG 5 cut(s) 183, 1471, 2300, 2847, 3366
AjuI GAANNNNNNNTTGG 2 cut(s) 3485, 3517
Alw21I GWGCWC 5 cut(s) 411, 2523, 2574, 3021, 3252
Alw26I GTCTC 4 cut(s) 2174, 2389, 2534, 3541
Alw44I GTGCAC 3 cut(s) 407, 3017, 3248
AlwI GGATC 8 cut(s) 314, 327, 446, 577, 802, 815, 2472, 3041
Ama87I CYCGRG 2 cut(s) 2165, 2522
Aor13HI TCCGGA 1 cut(s) 2566
Aor51HI AGCGCT 1 cut(s) 383
AoxI GGCC 1 cut(s) 2761
ApaLI GTGCAC 3 cut(s) 407, 3017, 3248
ApeKI GCWGC 4 cut(s) 5, 169, 437, 638
AseI ATTAAT 1 cut(s) 2069
Asp700I GAANNNNTTC 3 cut(s) 903, 1675, 2276
AspLEI GCGC 1 cut(s) 384
AspS9I GGNCC 2 cut(s) 868, 2651
AsuC2I CCSGG 2 cut(s) 912, 2177
AsuHPI GGTGA 3 cut(s) 2129, 2319, 2936
AvaI CYCGRG 2 cut(s) 2165, 2522
AvaII GGWCC 2 cut(s) 868, 2651
BaeGI GKGCMC 3 cut(s) 411, 3021, 3252
BaeI ACNNNNGTAYC 4 cut(s) 2619, 2652, 3386, 3419
BamHI GGATCC 2 cut(s) 319, 807
BanI GGYRCC 1 cut(s) 3394
BanII GRGCYC 1 cut(s) 2574
BauI CACGAG 1 cut(s) 410
BbsI GAAGAC 2 cut(s) 1125, 3122
Bbv12I GWGCWC 5 cut(s) 411, 2523, 2574, 3021, 3252
BbvI GCAGC 3 cut(s) 156, 424, 625
BccI CCATC 9 cut(s) 22, 231, 278, 331, 391, 1677, 2948, 2964, 3049
BcgI CGANNNNNNTGC 4 cut(s) 1019, 1053, 2146, 2180
BciT130I CCWGG 5 cut(s) 185, 1473, 2302, 2849, 3368
BciVI GTATCC 1 cut(s) 3344
BclI TGATCA 3 cut(s) 79, 1407, 2724
BcnI CCSGG 2 cut(s) 912, 2177
BcoDI GTCTC 4 cut(s) 2174, 2389, 2534, 3541
BfaI CTAG 3 cut(s) 936, 1367, 1922
BfmI CTRYAG 3 cut(s) 88, 170, 1077
BfoI RGCGCY 1 cut(s) 385
BfrI CTTAAG 1 cut(s) 121
BfuI GTATCC 1 cut(s) 3344
BglI GCCNNNNNGGC 1 cut(s) 446
BglII AGATCT 1 cut(s) 422
BisI GCNGC 6 cut(s) 6, 25, 170, 438, 639, 2676
BlpI GCTNAGC 1 cut(s) 3161
BlsI GCNGC 6 cut(s) 7, 26, 171, 439, 640, 2677
BmcAI AGTACT 1 cut(s) 1323
Bme1390I CCNGG 7 cut(s) 185, 912, 1473, 2177, 2302, 2849, 3368
Bme18I GGWCC 2 cut(s) 868, 2651
BmeT110I CYCGRG 2 cut(s) 2165, 2522
BmgBI CACGTC 1 cut(s) 3551
BmgT120I GGNCC 2 cut(s) 868, 2651
BmiI GGNNCC 7 cut(s) 321, 489, 809, 2652, 2778, 3396, 3521
BmrFI CCNGG 7 cut(s) 185, 912, 1473, 2177, 2302, 2849, 3368
BmsI GCATC 6 cut(s) 11, 424, 1492, 1637, 1773, 2410
BpiI GAAGAC 2 cut(s) 1125, 3122
BplI GAGNNNNNCTC 2 cut(s) 409, 441
BpmI CTGGAG 4 cut(s) 2700, 2805, 2831, 3007
Bpu1102I GCTNAGC 1 cut(s) 3161
BpuEI CTTGAG 5 cut(s) 217, 1034, 2081, 2144, 2951
BpuMI CCSGG 2 cut(s) 912, 2177
BsaAI YACGTR 1 cut(s) 3388
BsaBI GATNNNNATC 1 cut(s) 3045
BsaJI CCNNGG 3 cut(s) 1471, 2848, 3366
BsaWI WCCGGW 1 cut(s) 2566
BsaXI ACNNNNNCTCC 4 cut(s) 1137, 1167, 2673, 2703
Bsc4I CCNNNNNNNGG 8 cut(s) 237, 877, 1352, 1734, 2646, 2786, 2849, 3056
Bse1I ACTGG 5 cut(s) 302, 349, 489, 2352, 2466
Bse3DI GCAATG 3 cut(s) 627, 2091, 2607
Bse8I GATNNNNATC 1 cut(s) 3045
BseAI TCCGGA 1 cut(s) 2566
BseBI CCWGG 5 cut(s) 185, 1473, 2302, 2849, 3368
BseDI CCNNGG 3 cut(s) 1471, 2848, 3366
BseJI GATNNNNATC 1 cut(s) 3045
BseLI CCNNNNNNNGG 8 cut(s) 237, 877, 1352, 1734, 2646, 2786, 2849, 3056
BseMI GCAATG 3 cut(s) 627, 2091, 2607
BseMII CTCAG 4 cut(s) 32, 2020, 2088, 2784
BseNI ACTGG 5 cut(s) 302, 349, 489, 2352, 2466
BseRI GAGGAG 3 cut(s) 162, 407, 2810
BseSI GKGCMC 3 cut(s) 411, 3021, 3252
BseXI GCAGC 3 cut(s) 156, 424, 625
BseYI CCCAGC 1 cut(s) 590
Bsh1236I CGCG 3 cut(s) 1203, 2320, 2933
BshFI GGCC 1 cut(s) 2763
BshNI GGYRCC 1 cut(s) 3394
BsiHKAI GWGCWC 5 cut(s) 411, 2523, 2574, 3021, 3252
BsiHKCI CYCGRG 2 cut(s) 2165, 2522
BsiSI CCGG 4 cut(s) 805, 911, 2177, 2567
BslFI GGGAC 4 cut(s) 1444, 2826, 2865, 3573
BslI CCNNNNNNNGG 8 cut(s) 237, 877, 1352, 1734, 2646, 2786, 2849, 3056
BsmAI GTCTC 4 cut(s) 2174, 2389, 2534, 3541
BsmBI CGTCTC 2 cut(s) 2534, 3541
BsmFI GGGAC 4 cut(s) 1444, 2826, 2865, 3573
BsnI GGCC 1 cut(s) 2763
BsoBI CYCGRG 2 cut(s) 2165, 2522
Bsp1286I GDGCHC 5 cut(s) 411, 2523, 2574, 3021, 3252
Bsp13I TCCGGA 1 cut(s) 2566
Bsp1407I TGTACA 4 cut(s) 1816, 2609, 2766, 2997
Bsp1720I GCTNAGC 1 cut(s) 3161
Bsp68I TCGCGA 1 cut(s) 1203
BspACI CCGC 7 cut(s) 25, 518, 965, 2647, 2676, 2931, 3198
BspANI GGCC 1 cut(s) 2763
BspCNI CTCAG 4 cut(s) 33, 2019, 2089, 2785
BspEI TCCGGA 1 cut(s) 2566
BspFNI CGCG 3 cut(s) 1203, 2320, 2933
BspHI TCATGA 2 cut(s) 264, 1410
BspLI GGNNCC 7 cut(s) 321, 489, 809, 2652, 2778, 3396, 3521
BspMAI CTGCAG 1 cut(s) 174
BspPI GGATC 8 cut(s) 314, 327, 446, 577, 802, 815, 2472, 3041
BspQI GCTCTTC 2 cut(s) 372, 2247
BspT107I GGYRCC 1 cut(s) 3394
BspTI CTTAAG 1 cut(s) 121
BsrDI GCAATG 3 cut(s) 627, 2091, 2607
BsrGI TGTACA 4 cut(s) 1816, 2609, 2766, 2997
BsrI ACTGG 5 cut(s) 302, 349, 489, 2352, 2466
BssECI CCNNGG 3 cut(s) 1471, 2848, 3366
BssSI CACGAG 1 cut(s) 410
Bst2BI CACGAG 1 cut(s) 410
Bst2UI CCWGG 5 cut(s) 185, 1473, 2302, 2849, 3368
Bst4CI ACNGT 9 cut(s) 1321, 1491, 1531, 1607, 2391, 2518, 2658, 2824, 2947
Bst6I CTCTTC 6 cut(s) 372, 1449, 1653, 2208, 2247, 2501
BstAFI CTTAAG 1 cut(s) 121
BstAPI GCANNNNNTGC 1 cut(s) 638
BstAUI TGTACA 4 cut(s) 1816, 2609, 2766, 2997
BstBAI YACGTR 1 cut(s) 3388
BstC8I GCNNGC 6 cut(s) 255, 731, 1032, 3282, 3414, 3418
BstDEI CTNAG 8 cut(s) 41, 1220, 2006, 2097, 2435, 2468, 2793, 3161
BstFNI CGCG 3 cut(s) 1203, 2320, 2933
BstH2I RGCGCY 1 cut(s) 385
BstHHI GCGC 1 cut(s) 384
BstMAI GTCTC 4 cut(s) 2174, 2389, 2534, 3541
BstMWI GCNNNNNNNGC 7 cut(s) 158, 446, 638, 2246, 2255, 2569, 2874
BstNI CCWGG 5 cut(s) 185, 1473, 2302, 2849, 3368
BstNSI RCATGY 5 cut(s) 556, 1034, 2707, 2841, 3327
BstSCI CCNGG 7 cut(s) 183, 910, 1471, 2175, 2300, 2847, 3366
BstSFI CTRYAG 3 cut(s) 88, 170, 1077
BstSLI GKGCMC 3 cut(s) 411, 3021, 3252
BstUI CGCG 3 cut(s) 1203, 2320, 2933
BstV1I GCAGC 3 cut(s) 156, 424, 625
BstV2I GAAGAC 2 cut(s) 1125, 3122
BstX2I RGATCY 5 cut(s) 319, 422, 807, 2464, 3046
BstYI RGATCY 5 cut(s) 319, 422, 807, 2464, 3046
BsuI GTATCC 1 cut(s) 3344
BsuRI GGCC 1 cut(s) 2763
BtgZI GCGATG 1 cut(s) 1184
BtrI CACGTC 1 cut(s) 3551
BtsIMutI CAGTG 5 cut(s) 1653, 1831, 2014, 2943, 3127
BtuMI TCGCGA 1 cut(s) 1203
Cac8I GCNNGC 6 cut(s) 255, 731, 1032, 3282, 3414, 3418
CciI TCATGA 2 cut(s) 264, 1410
CfoI GCGC 1 cut(s) 384
Cfr13I GGNCC 2 cut(s) 868, 2651
CseI GACGC 1 cut(s) 2516
DdeI CTNAG 8 cut(s) 41, 1220, 2006, 2097, 2435, 2468, 2793, 3161
DraI TTTAAA 1 cut(s) 1636
Eam1104I CTCTTC 6 cut(s) 372, 1449, 1653, 2208, 2247, 2501
EarI CTCTTC 6 cut(s) 372, 1449, 1653, 2208, 2247, 2501
Ecl136II GAGCTC 1 cut(s) 2572
Eco24I GRGCYC 1 cut(s) 2574
Eco32I GATATC 2 cut(s) 754, 1207
Eco47I GGWCC 2 cut(s) 868, 2651
Eco47III AGCGCT 1 cut(s) 383
Eco53kI GAGCTC 1 cut(s) 2572
Eco57I CTGAAG 7 cut(s) 687, 1677, 1869, 2189, 2263, 3372, 3453
Eco88I CYCGRG 2 cut(s) 2165, 2522
EcoICRI GAGCTC 1 cut(s) 2572
EcoRI GAATTC 1 cut(s) 111
EcoRII CCWGG 5 cut(s) 183, 1471, 2300, 2847, 3366
EcoRV GATATC 2 cut(s) 754, 1207
EcoT22I ATGCAT 1 cut(s) 1507
EcoT38I GRGCYC 1 cut(s) 2574
Esp3I CGTCTC 2 cut(s) 2534, 3541
FaqI GGGAC 4 cut(s) 1444, 2826, 2865, 3573
FauI CCCGC 2 cut(s) 2640, 3191
FbaI TGATCA 3 cut(s) 79, 1407, 2724
FblI GTMKAC 2 cut(s) 1486, 2491
Fnu4HI GCNGC 6 cut(s) 6, 25, 170, 438, 639, 2676
FokI GGATG 9 cut(s) 260, 310, 894, 1035, 1199, 1508, 1514, 1795, 2943
FriOI GRGCYC 1 cut(s) 2574
Fsp4HI GCNGC 6 cut(s) 6, 25, 170, 438, 639, 2676
FspBI CTAG 3 cut(s) 936, 1367, 1922
GlaI GCGC 1 cut(s) 383
GluI GCNGC 6 cut(s) 6, 25, 170, 438, 639, 2676
GsaI CCCAGC 1 cut(s) 594
GsuI CTGGAG 4 cut(s) 2700, 2805, 2831, 3007
HaeII RGCGCY 1 cut(s) 385
HaeIII GGCC 1 cut(s) 2763
HapII CCGG 4 cut(s) 805, 911, 2177, 2567
HgaI GACGC 1 cut(s) 2516
HhaI GCGC 1 cut(s) 384
Hin6I GCGC 1 cut(s) 382
HinP1I GCGC 1 cut(s) 382
HincII GTYRAC 1 cut(s) 2196
HindII GTYRAC 1 cut(s) 2196
HindIII AAGCTT 2 cut(s) 1228, 2470
HpaII CCGG 4 cut(s) 805, 911, 2177, 2567
HphI GGTGA 3 cut(s) 2129, 2319, 2936
Hpy188I TCNGA 9 cut(s) 342, 706, 751, 1612, 1747, 1768, 1888, 2419, 3051
HpyCH4III ACNGT 9 cut(s) 1321, 1491, 1531, 1607, 2391, 2518, 2658, 2824, 2947
HpyCH4IV ACGT 7 cut(s) 271, 816, 1480, 2553, 3211, 3387, 3550
HpyF10VI GCNNNNNNNGC 7 cut(s) 158, 446, 638, 2246, 2255, 2569, 2874
HpyF3I CTNAG 8 cut(s) 41, 1220, 2006, 2097, 2435, 2468, 2793, 3161
HpySE526I ACGT 7 cut(s) 271, 816, 1480, 2553, 3211, 3387, 3550
HspAI GCGC 1 cut(s) 382
Kpn2I TCCGGA 1 cut(s) 2566
Ksp22I TGATCA 3 cut(s) 79, 1407, 2724
LguI GCTCTTC 2 cut(s) 372, 2247
LmnI GCTCC 7 cut(s) 149, 2178, 2209, 2569, 2577, 2797, 3277
Lsp1109I GCAGC 3 cut(s) 156, 424, 625
LweI GCATC 6 cut(s) 11, 424, 1492, 1637, 1773, 2410
MaeI CTAG 3 cut(s) 936, 1367, 1922
MaeII ACGT 7 cut(s) 271, 816, 1480, 2553, 3211, 3387, 3550
MaeIII GTNAC 6 cut(s) 659, 1835, 2026, 2626, 2824, 3358
MfeI CAATTG 2 cut(s) 2184, 3513
MflI RGATCY 5 cut(s) 319, 422, 807, 2464, 3046
MhlI GDGCHC 5 cut(s) 411, 2523, 2574, 3021, 3252
MlyI GAGTC 5 cut(s) 1466, 2043, 2497, 2518, 2847
MmeI TCCRAC 5 cut(s) 502, 1164, 1599, 2677, 3313
Mph1103I ATGCAT 1 cut(s) 1507
MroI TCCGGA 1 cut(s) 2566
MroXI GAANNNNTTC 3 cut(s) 903, 1675, 2276
MslI CAYNNNNRTG 3 cut(s) 19, 2744, 3255
MspA1I CMGCKG 1 cut(s) 2678
MspCI CTTAAG 1 cut(s) 121
MspI CCGG 4 cut(s) 805, 911, 2177, 2567
MspR9I CCNGG 7 cut(s) 185, 912, 1473, 2177, 2302, 2849, 3368
MunI CAATTG 2 cut(s) 2184, 3513
MvaI CCWGG 5 cut(s) 185, 1473, 2302, 2849, 3368
MvnI CGCG 3 cut(s) 1203, 2320, 2933
MwoI GCNNNNNNNGC 7 cut(s) 158, 446, 638, 2246, 2255, 2569, 2874
NciI CCSGG 2 cut(s) 912, 2177
NlaIV GGNNCC 7 cut(s) 321, 489, 809, 2652, 2778, 3396, 3521
NmuCI GTSAC 1 cut(s) 1835
NruI TCGCGA 1 cut(s) 1203
NsiI ATGCAT 1 cut(s) 1507
NspI RCATGY 5 cut(s) 556, 1034, 2707, 2841, 3327
PaeI GCATGC 1 cut(s) 1034
PaeR7I CTCGAG 2 cut(s) 2165, 2522
PagI TCATGA 2 cut(s) 264, 1410
PciI ACATGT 2 cut(s) 552, 2837
PciSI GCTCTTC 2 cut(s) 372, 2247
PdmI GAANNNNTTC 3 cut(s) 903, 1675, 2276
PflMI CCANNNNNTGG 1 cut(s) 1352
PfoI TCCNGGA 4 cut(s) 183, 910, 2175, 2300
PkrI GCNGC 6 cut(s) 7, 26, 171, 439, 640, 2677
PleI GAGTC 5 cut(s) 1465, 2043, 2496, 2517, 2847
PpsI GAGTC 5 cut(s) 1465, 2043, 2496, 2517, 2847
Ppu21I YACGTR 1 cut(s) 3388
PscI ACATGT 2 cut(s) 552, 2837
PshBI ATTAAT 1 cut(s) 2069
PsiI TTATAA 1 cut(s) 645
Psp124BI GAGCTC 1 cut(s) 2574
Psp6I CCWGG 5 cut(s) 183, 1471, 2300, 2847, 3366
PspFI CCCAGC 1 cut(s) 590
PspGI CCWGG 5 cut(s) 183, 1471, 2300, 2847, 3366
PspN4I GGNNCC 7 cut(s) 321, 489, 809, 2652, 2778, 3396, 3521
PspPI GGNCC 2 cut(s) 868, 2651
PspXI VCTCGAGB 1 cut(s) 2522
PstI CTGCAG 1 cut(s) 174
PsuI RGATCY 5 cut(s) 319, 422, 807, 2464, 3046
RruI TCGCGA 1 cut(s) 1203
RseI CAYNNNNRTG 3 cut(s) 19, 2744, 3255
SacI GAGCTC 1 cut(s) 2574
SapI GCTCTTC 2 cut(s) 372, 2247
SatI GCNGC 6 cut(s) 6, 25, 170, 438, 639, 2676
Sau96I GGNCC 2 cut(s) 868, 2651
ScaI AGTACT 1 cut(s) 1323
SchI GAGTC 5 cut(s) 1466, 2043, 2497, 2518, 2847
ScrFI CCNGG 7 cut(s) 185, 912, 1473, 2177, 2302, 2849, 3368
SduI GDGCHC 5 cut(s) 411, 2523, 2574, 3021, 3252
SfaNI GCATC 6 cut(s) 11, 424, 1492, 1637, 1773, 2410
SfcI CTRYAG 3 cut(s) 88, 170, 1077
Sfr274I CTCGAG 2 cut(s) 2165, 2522
SinI GGWCC 2 cut(s) 868, 2651
SlaI CTCGAG 2 cut(s) 2165, 2522
SmiMI CAYNNNNRTG 3 cut(s) 19, 2744, 3255
SmlI CTYRAG 8 cut(s) 121, 232, 1013, 2060, 2123, 2165, 2522, 2966
SmoI CTYRAG 8 cut(s) 121, 232, 1013, 2060, 2123, 2165, 2522, 2966
SphI GCATGC 1 cut(s) 1034
SsiI CCGC 7 cut(s) 25, 518, 965, 2647, 2676, 2931, 3198
SspI AATATT 5 cut(s) 460, 2758, 2899, 3233, 3439
SspMI CTAG 3 cut(s) 936, 1367, 1922
SstI GAGCTC 1 cut(s) 2574
StyD4I CCNGG 7 cut(s) 183, 910, 1471, 2175, 2300, 2847, 3366
TaaI ACNGT 9 cut(s) 1321, 1491, 1531, 1607, 2391, 2518, 2658, 2824, 2947
TaiI ACGT 7 cut(s) 274, 819, 1483, 2556, 3214, 3390, 3553
TaqI TCGA 6 cut(s) 1039, 1120, 1371, 2166, 2523, 3454
TatI WGTACW 8 cut(s) 1087, 1321, 1816, 2609, 2766, 2916, 2997, 3480
TauI GCSGC 2 cut(s) 27, 2678
TscAI CASTG 5 cut(s) 1660, 1831, 2014, 2950, 3127
TseFI GTSAC 1 cut(s) 1835
TseI GCWGC 4 cut(s) 5, 169, 437, 638
Tsp45I GTSAC 1 cut(s) 1835
TspGWI ACGGA 3 cut(s) 1098, 2522, 3459
TspRI CASTG 5 cut(s) 1660, 1831, 2014, 2950, 3127
Van91I CCANNNNNTGG 1 cut(s) 1352
Vha464I CTTAAG 1 cut(s) 121
VneI GTGCAC 3 cut(s) 407, 3017, 3248
VpaK11BI GGWCC 2 cut(s) 868, 2651
VspI ATTAAT 1 cut(s) 2069
XceI RCATGY 5 cut(s) 556, 1034, 2707, 2841, 3327
XcmI CCANNNNNNNNNTGG 1 cut(s) 2359
XhoI CTCGAG 2 cut(s) 2165, 2522
XmiI GTMKAC 2 cut(s) 1486, 2491
XmnI GAANNNNTTC 3 cut(s) 903, 1675, 2276
XspI CTAG 3 cut(s) 936, 1367, 1922
ZrmI AGTACT 1 cut(s) 1323
Zsp2I ATGCAT 1 cut(s) 1507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.