Rw4G010650

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000654
Physical Location & Seq
Forward (+)
24791528 .. 24795705
4178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G010650.1

Sequence Viewer

Length: 3537 bp
ATGAGCACACAGTTGGATGCTTCTTCTTCTTCCTCGCCTTCCCCTTACCGGTGTACTTATCATGCATTCTTGAGTTTCAGAGGTAAGGACACACGTAAGGGATTTACCGATCATCTTTACAGGGCCTTGGAGCTCGCAGGAATCCACACGTTTAGAGACGATGATGAAATCGAGAGAGGAGCAAATATCGCAGCAGAGTTACAGAGAGCTATCCAAGAGTCGAGGGTATCTGTGGTTGTGTTGTCTAGAGACTATGCTTCATCAAGGTGGTGCTTGGACGAACTTGCAAAGATCATGGAACGTAGGAGAAATGATGACGCACACGTGGTTATTCCAGTGTTTTATGATGTAGATCCTTCCCATGTCAGGAAGCAAATCGGGAGTTTTGCTCAAGCTTTTGTTAGACATGAAGAGCGCTTCAAGGAGGAGATGGGAAAGGTTGCCGAGTGGAGAAGAGCGCTTTCTGATATTGCAGATCTTGGTGGAATGGTTTTAGGACCACGGTATGAATCACAATTTATCCAAGATATTGTTGAAGAAATCAGAAGTAAATTGGATTCCAAAGTCTTAAACATTGCTCCGTATGCAATTGGAATAGATGATCGTGTGCGAGGTATAAACATGTGGTTAGAAGATGGGTCAAATGATGTTGGTGTGGCTGTGATCTATGGAATGGGTGGAATTGGCAAAACCACTATTGCCAAAGCTGCTTATAACTTGAATTTCCATAGATTTCAAGGTAGCAGCTTTCTTGCTGATGTTAGAGAAACTTCAGAACAGCCGAATGGGGAAAACAAAGAAAATTACAACATTGATGAAGGAATACGAAAGATAAAAGATGCTGTATGTTGCAAGAAAGTTCTTATTGTTCTTGATGATGTGAACCACTCAGACCAATTTAATGCAATTATTGGAATGCGCAGATGGTTTGTTCCAGGAAGTAAGATAATCTTAACAACCAGATACGAACATTTGTTAAAGGCTCACGAAGTTAACACAATGTTTAAGCTCCTCAGTTGGCACGCCTTTGGACAAGCCCATCCTATTGAAGCTTACATTGATCTTTCAAAACCCGTTGTAAAACATTGTGGAGGGATTCCATTAGCTCTGCAAGTTCTGGGATCTTCTTTGTCTGGCAGAAGTATAGATATATGGCAAAGCGCATTACAGAAGCTATGTGAGATTCCAGATGGAGAAATTCAAAAAATTCTTAGAATCAGTTTTGACTCTCTGCAAGATGATCATGACAAAAACTTATTCCTTCATATAGCATGCTTCTTCAGAGGGAAGAATAAAGAGTTTTCAATTACAGTACTGGACAACCTTAAATTCTACACAGTGATTGGAATTCAAAATCTGGTTGATAGATGTCTAGTGAAAATTAATGAAGACAACAAGTTGATAGTGCATCATTTGCTTCGAGACATGGCAAGGGGAATTATTCGCGAAGAATCACCTGAGGACCCTGGGAAACGCAGTAGGGTGTGGCACAAAGATGCCTTCAATATTTTGAGTAAAAGAACTGTAAGAAACATAATCTGCATGTCAGGGTACTGGACTGTTAAGGGCATCATGCTTGACCTTTCTATGTTGATGCAAGATGAGTCTTCCAAAACATTATTTAGTGGAAGTAACAGCAAACGACGTCGTGTTGATGATTATGATGGAAATTGTTCAGGACGGCGTCGACTTGGTCTCTTCACCTGGCAATTATTTAGTTTTTCCTCAGCCAACACGACTCCAGCATCAGATGAGGTTGACCTCAAAATAGAGGCATTCAAAAAGATGCACAACCTTGAAGTTCTCCTACTCAATAATGTACATTTCAGTCAAGGCTTTGAAAATTTTCCAAAAAACTTGATATGGCTGTCTTGGCGAGGATTCTCTTTAAATTCGATACCAGCCAGTTTGTATTTGAAAACTCTAGTTGTTCTTGACTTGCGGAATAGCAGCCTGCAATGTGTTTGGAAGGGAACCAAGTCTCTTCCAAGTCTGAAAGTCCTTAATCTTAGTCATTCACATGGCCTTTCAGCAACCCCTGACATATCAGGACTCCCTAACCTGGAGAGATTGATTCTAAAAAATTGTAGAAATTTGATTGAGGTTGATGAATCGATTGGAGACTTGGAGAAACTTGTTTTCTTAAATCTAAAAGACTGCAAAAATCTGATGAAGCTTCCGACCAGAATCAAAATGTCAAGTTCTCTTCAAACACTTATACTTTCTGGTTGCTCAAAGCTTGTGCTTCATTCTAATATGGTTGCAACAAATCAGTCACTCTTGACAGCTAGTGATATGAAGAAAGACGATTGTTCATCACCTAACTTGTGGAAATCACTCTTATTATGGGCAGTACCAAGCAGAAATATCATACCAACCAGTTTCTCAGTATCAAATTTACCACGTTCTTTACGAAGCTTAAGCTTAGCTGACTGCAACCTATTAGAGATTCCAGGTGATCTTAGTATCCTCTCCTCATTGAAGTATTTAGACCTATGTGGGAACCCAATTCAGAGCCTCCCAGAAAACATAAGATCTCTTAGTAAGCTTGAGACACTTATGTTAGAGGACTGCACAGAACTCAGAATGCTTCCAGAGCTCCCACCAAGTCTCCAGAGTTTGCTTGCAAGTTCTTGTAAATCATTGAAAAGAATAACAAATTTACCAAACTTGTTCAAATCATTGGATACAGATTTTCTGGATTGCAAGAAGTTAGTTGAAGTTGAAAGCTTGTTCACTATAAAACCATTGACAACCACTGACATAGAAATGATCAAAGATATGGGACTGTTCAATTTTAAATCCATTGGAAGCGACGCTGAGGTTGAAATGATCAACTACATTACAAATACAACCAAGAAGGGCCCCCTCCAGGTGTTGTCTGAATGTGGCATATCTAGCATTTACCTAAACGGAAACGAGATTCCAGATTGGTTTTGCCACAGGAGAATGGGCAACTCTGTGTTGTCTATTATAGTACCTCCACATCTTAATCATAGAATTCGAGGCTTAAATGCATGTGTTGTATATGCACGACGCCCAGATGTTGGTCGTTCTGCTAAATATCAAAACTTGCTTAAAGTCAGCAATGAGACCAAGGGTCTCATGTGGACCTATTCCCCTATCACAATAGGCATTCCAAAGGAGAATGAAGACATGTTATGGCTAAGCCATTGGCGGTTTGGAGAACATGATCTGGGAGGGGGAGACGAAGTACGTGTTTCGGTTGAGTTTGGATCCGAGCTTGGACACAATCTAAATTTATGGACAAAGGAATTGGGAATCCAACTTGTGTATGAGCAGGAAAGTAGTGAAGATGGAAAGAAACACAATTTCCTTGCTGGAGATGTGTCAGTGTCATCCTCAAGTTACCAAATGTGGACGGGAAAATACTTTCTTTGTAATAACCTGAATCGTGCTCAGAAAACACAATTCAAGAGTAGCCAGGAAAACCCATCTCATCTTGATTCTGACAAAACCAAGAGCCCCTTCCTTTTCTTTTTTGAGCATGATGATGTTTCACAGGCTGATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1178

Amino Acids

133.27

Weight (kDa)

7.36

Isoelectric Point (pI)

46.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 188 6.1e-54 TIR domain
TIR_2 PF13676 23 - 133 3.8e-18 TIR domain
NB-ARC PF00931 201 - 331 1.1e-11 NB-ARC domain
WHD_ROQ1 PF23282 414 - 485 5.7e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 632 - 743 6.9e-06 Leucine-rich repeat region
LRR_14 PF23598 802 - 895 4.5e-08 Leucine-rich repeat region
LRR_8 PF13855 802 - 858 2.5e-06 Leucine rich repeat
C-JID PF20160 971 - 1103 5.1e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 714
AatII GACGTC 1 cut(s) 1648
Acc16I TGCGCA 1 cut(s) 920
AccB7I CCANNNNNTGG 1 cut(s) 3047
AccI GTMKAC 1 cut(s) 1687
AccII CGCG 1 cut(s) 1446
AciI CCGC 2 cut(s) 1942, 3178
AclWI GGATC 4 cut(s) 347, 1129, 3231, 3244
AcuI CTGAAG 2 cut(s) 756, 1264
AcvI CACGTG 1 cut(s) 325
AcyI GRCGYC 3 cut(s) 1645, 1684, 3037
AfaI GTAC 7 cut(s) 55, 1314, 1553, 1821, 2355, 2979, 3216
AfeI AGCGCT 2 cut(s) 416, 459
AfiI CCNNNNNNNGG 5 cut(s) 48, 366, 2062, 2872, 3047
AflII CTTAAG 1 cut(s) 2419
AflIII ACRYGT 6 cut(s) 92, 147, 322, 621, 3156, 3217
AgeI ACCGGT 1 cut(s) 48
AhdI GACNNNNNGTC 1 cut(s) 3099
AjnI CCWGG 7 cut(s) 934, 1465, 1703, 2061, 2452, 2871, 3444
AjuI GAANNNNNNNTTGG 2 cut(s) 3473, 3505
Alw21I GWGCWC 4 cut(s) 8, 135, 2601, 3421
AlwI GGATC 4 cut(s) 347, 1129, 3231, 3244
Aor51HI AGCGCT 2 cut(s) 416, 459
AoxI GGCC 3 cut(s) 123, 2023, 2863
ApaI GGGCCC 1 cut(s) 2867
ApeKI GCWGC 4 cut(s) 191, 707, 744, 1950
AseI ATTAAT 1 cut(s) 1383
AsiGI ACCGGT 1 cut(s) 48
Asp700I GAANNNNTTC 2 cut(s) 1672, 1845
AspLEI GCGC 4 cut(s) 417, 460, 921, 1163
AspS9I GGNCC 6 cut(s) 123, 497, 1462, 2863, 2864, 3111
AsuHPI GGTGA 4 cut(s) 1446, 1693, 2310, 2468
AvaII GGWCC 3 cut(s) 497, 1462, 3111
AxyI CCTNAGG 1 cut(s) 1458
BaeGI GKGCMC 1 cut(s) 2867
BamHI GGATCC 1 cut(s) 3236
BanII GRGCYC 4 cut(s) 135, 2601, 2867, 3488
BarI GAAGNNNNNNTAC 2 cut(s) 1791, 1823
BbrPI CACGTG 1 cut(s) 325
BbsI GAAGAC 3 cut(s) 1395, 1599, 3159
Bbv12I GWGCWC 4 cut(s) 8, 135, 2601, 3421
BbvCI CCTCAGC 2 cut(s) 1726, 2820
BbvI GCAGC 4 cut(s) 203, 694, 756, 1962
BccI CCATC 8 cut(s) 424, 629, 918, 1047, 1184, 1658, 3311, 3463
BceAI ACGGC 1 cut(s) 1697
BciT130I CCWGG 7 cut(s) 936, 1467, 1705, 2063, 2454, 2873, 3446
BciVI GTATCC 2 cut(s) 2477, 2680
BclI TGATCA 4 cut(s) 1240, 2772, 2832, 3529
BfaI CTAG 5 cut(s) 246, 1373, 1925, 2289, 2898
BfoI RGCGCY 2 cut(s) 418, 461
BfrI CTTAAG 1 cut(s) 2419
BfuI GTATCC 2 cut(s) 2477, 2680
BglII AGATCT 2 cut(s) 475, 2534
BisI GCNGC 4 cut(s) 192, 708, 745, 1951
BlpI GCTNAGC 2 cut(s) 2425, 3167
BlsI GCNGC 4 cut(s) 193, 709, 746, 1952
BmcAI AGTACT 1 cut(s) 1314
Bme1390I CCNGG 7 cut(s) 936, 1467, 1705, 2063, 2454, 2873, 3446
Bme18I GGWCC 3 cut(s) 497, 1462, 3111
BmeRI GACNNNNNGTC 1 cut(s) 3099
BmgT120I GGNCC 6 cut(s) 123, 497, 1462, 2863, 2864, 3111
BmiI GGNNCC 6 cut(s) 1464, 1975, 2504, 2865, 2866, 3238
BmrFI CCNGG 7 cut(s) 936, 1467, 1705, 2063, 2454, 2873, 3446
BmsI GCATC 8 cut(s) 7, 829, 1417, 1486, 1578, 1584, 1754, 1776
BpiI GAAGAC 3 cut(s) 1395, 1599, 3159
BplI GAGNNNNNCTC 2 cut(s) 373, 405
BpmI CTGGAG 5 cut(s) 1725, 2084, 2597, 2855, 3363
Bpu10I CCTNAGC 2 cut(s) 1726, 2820
Bpu1102I GCTNAGC 2 cut(s) 2425, 3167
BpuEI CTTGAG 4 cut(s) 91, 375, 2570, 3349
Bsa29I ATCGAT 1 cut(s) 2114
BsaAI YACGTR 3 cut(s) 95, 325, 3218
BsaBI GATNNNNATC 1 cut(s) 351
BsaHI GRCGYC 3 cut(s) 1645, 1684, 3037
BsaI GGTCTC 3 cut(s) 1700, 3086, 3107
BsaJI CCNNGG 5 cut(s) 126, 500, 1465, 1466, 3096
BsaWI WCCGGW 1 cut(s) 48
BsaXI ACNNNNNCTCC 4 cut(s) 2595, 2625, 3198, 3228
Bsc4I CCNNNNNNNGG 5 cut(s) 48, 366, 2062, 2872, 3047
Bse118I RCCGGY 1 cut(s) 48
Bse1I ACTGG 5 cut(s) 335, 1320, 1559, 1905, 2379
Bse21I CCTNAGG 1 cut(s) 1458
Bse3DI GCAATG 3 cut(s) 573, 1964, 3094
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 7 cut(s) 936, 1467, 1705, 2063, 2454, 2873, 3446
BseCI ATCGAT 1 cut(s) 2114
BseDI CCNNGG 5 cut(s) 126, 500, 1465, 1466, 3096
BseGI GGATG 3 cut(s) 22, 1039, 3359
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 5 cut(s) 48, 366, 2062, 2872, 3047
BseMI GCAATG 3 cut(s) 573, 1964, 3094
BseMII CTCAG 8 cut(s) 903, 1027, 1449, 1740, 2400, 2596, 2811, 3434
BseNI ACTGG 5 cut(s) 335, 1320, 1559, 1905, 2379
BseRI GAGGAG 4 cut(s) 192, 440, 1001, 2464
BseSI GKGCMC 1 cut(s) 2867
BseXI GCAGC 4 cut(s) 203, 694, 756, 1962
BsgI GTGCAG 1 cut(s) 2557
Bsh1236I CGCG 1 cut(s) 1446
BshFI GGCC 3 cut(s) 125, 2025, 2865
BshTI ACCGGT 1 cut(s) 48
BshVI ATCGAT 1 cut(s) 2114
BsiHKAI GWGCWC 4 cut(s) 8, 135, 2601, 3421
BsiSI CCGG 1 cut(s) 49
BslFI GGGAC 1 cut(s) 2799
BslI CCNNNNNNNGG 5 cut(s) 48, 366, 2062, 2872, 3047
BsmBI CGTCTC 2 cut(s) 150, 3201
BsmFI GGGAC 1 cut(s) 2799
BsmI GAATGC 5 cut(s) 65, 921, 1775, 2592, 3135
BsnI GGCC 3 cut(s) 125, 2025, 2865
Bso31I GGTCTC 3 cut(s) 1700, 3086, 3107
Bsp120I GGGCCC 1 cut(s) 2863
Bsp1286I GDGCHC 6 cut(s) 8, 135, 2601, 2867, 3421, 3488
Bsp1407I TGTACA 1 cut(s) 1819
Bsp1720I GCTNAGC 2 cut(s) 2425, 3167
Bsp68I TCGCGA 1 cut(s) 1446
BspACI CCGC 2 cut(s) 1942, 3178
BspANI GGCC 3 cut(s) 125, 2025, 2865
BspCNI CTCAG 8 cut(s) 902, 1026, 1450, 1739, 2399, 2595, 2812, 3433
BspDI ATCGAT 1 cut(s) 2114
BspFNI CGCG 1 cut(s) 1446
BspHI TCATGA 1 cut(s) 1243
BspLI GGNNCC 6 cut(s) 1464, 1975, 2504, 2865, 2866, 3238
BspPI GGATC 4 cut(s) 347, 1129, 3231, 3244
BspQI GCTCTTC 2 cut(s) 405, 448
BspTI CTTAAG 1 cut(s) 2419
BspTNI GGTCTC 3 cut(s) 1700, 3086, 3107
BsrDI GCAATG 3 cut(s) 573, 1964, 3094
BsrFI RCCGGY 1 cut(s) 48
BsrGI TGTACA 1 cut(s) 1819
BsrI ACTGG 5 cut(s) 335, 1320, 1559, 1905, 2379
BssAI RCCGGY 1 cut(s) 48
BssECI CCNNGG 5 cut(s) 126, 500, 1465, 1466, 3096
BssNI GRCGYC 3 cut(s) 1645, 1684, 3037
BssT1I CCWWGG 2 cut(s) 126, 3096
Bst2UI CCWGG 7 cut(s) 936, 1467, 1705, 2063, 2454, 2873, 3446
Bst4CI ACNGT 7 cut(s) 12, 504, 1312, 1339, 1525, 1561, 2790
Bst6I CTCTTC 5 cut(s) 405, 448, 1703, 1989, 2211
BstACI GRCGYC 3 cut(s) 1645, 1684, 3037
BstAFI CTTAAG 1 cut(s) 2419
BstAPI GCANNNNNTGC 1 cut(s) 1414
BstAUI TGTACA 1 cut(s) 1819
BstBAI YACGTR 3 cut(s) 95, 325, 3218
BstC8I GCNNGC 5 cut(s) 135, 1023, 1273, 1955, 2625
BstDSI CCRYGG 1 cut(s) 500
BstF5I GGATG 3 cut(s) 22, 1039, 3359
BstFNI CGCG 1 cut(s) 1446
BstH2I RGCGCY 2 cut(s) 418, 461
BstHHI GCGC 4 cut(s) 417, 460, 921, 1163
BstMWI GCNNNNNNNGC 7 cut(s) 188, 584, 707, 1414, 1873, 2596, 2898
BstNI CCWGG 7 cut(s) 936, 1467, 1705, 2063, 2454, 2873, 3446
BstNSI RCATGY 5 cut(s) 625, 1275, 1546, 3021, 3160
BstSCI CCNGG 7 cut(s) 934, 1465, 1703, 2061, 2452, 2871, 3444
BstSLI GKGCMC 1 cut(s) 2867
BstUI CGCG 1 cut(s) 1446
BstV1I GCAGC 4 cut(s) 203, 694, 756, 1962
BstV2I GAAGAC 3 cut(s) 1395, 1599, 3159
BstX2I RGATCY 5 cut(s) 352, 475, 1121, 2534, 3236
BstYI RGATCY 5 cut(s) 352, 475, 1121, 2534, 3236
Bsu15I ATCGAT 1 cut(s) 2114
Bsu36I CCTNAGG 1 cut(s) 1458
BsuI GTATCC 2 cut(s) 2477, 2680
BsuRI GGCC 3 cut(s) 125, 2025, 2865
BsuTUI ATCGAT 1 cut(s) 2114
BtgI CCRYGG 1 cut(s) 500
BtsCI GGATG 3 cut(s) 22, 1039, 3359
BtsIMutI CAGTG 4 cut(s) 342, 1344, 2757, 3360
BtuMI TCGCGA 1 cut(s) 1446
Cac8I GCNNGC 5 cut(s) 135, 1023, 1273, 1955, 2625
CciI TCATGA 1 cut(s) 1243
CfoI GCGC 4 cut(s) 417, 460, 921, 1163
Cfr10I RCCGGY 1 cut(s) 48
Cfr13I GGNCC 6 cut(s) 123, 497, 1462, 2863, 2864, 3111
ClaI ATCGAT 1 cut(s) 2114
CseI GACGC 4 cut(s) 326, 1673, 2825, 3045
Csp6I GTAC 7 cut(s) 54, 1313, 1552, 1820, 2354, 2978, 3215
CspAI ACCGGT 1 cut(s) 48
CviQI GTAC 7 cut(s) 54, 1313, 1552, 1820, 2354, 2978, 3215
DraI TTTAAA 2 cut(s) 1890, 2800
DriI GACNNNNNGTC 1 cut(s) 3099
Eam1104I CTCTTC 5 cut(s) 405, 448, 1703, 1989, 2211
Eam1105I GACNNNNNGTC 1 cut(s) 3099
EarI CTCTTC 5 cut(s) 405, 448, 1703, 1989, 2211
Ecl136II GAGCTC 2 cut(s) 133, 2599
Eco130I CCWWGG 2 cut(s) 126, 3096
Eco24I GRGCYC 4 cut(s) 135, 2601, 2867, 3488
Eco31I GGTCTC 3 cut(s) 1700, 3086, 3107
Eco47I GGWCC 3 cut(s) 497, 1462, 3111
Eco47III AGCGCT 2 cut(s) 416, 459
Eco53kI GAGCTC 2 cut(s) 133, 2599
Eco57I CTGAAG 2 cut(s) 756, 1264
Eco72I CACGTG 1 cut(s) 325
Eco81I CCTNAGG 1 cut(s) 1458
EcoICRI GAGCTC 2 cut(s) 133, 2599
EcoO109I RGGNCCY 4 cut(s) 123, 1462, 2863, 2864
EcoRI GAATTC 2 cut(s) 1347, 3000
EcoRII CCWGG 7 cut(s) 934, 1465, 1703, 2061, 2452, 2871, 3444
EcoT14I CCWWGG 2 cut(s) 126, 3096
EcoT22I ATGCAT 2 cut(s) 67, 3019
EcoT38I GRGCYC 4 cut(s) 135, 2601, 2867, 3488
ErhI CCWWGG 2 cut(s) 126, 3096
Esp3I CGTCTC 2 cut(s) 150, 3201
FalI AAGNNNNNCTT 4 cut(s) 935, 967, 3473, 3505
FaqI GGGAC 1 cut(s) 2799
FbaI TGATCA 4 cut(s) 1240, 2772, 2832, 3529
FblI GTMKAC 1 cut(s) 1687
Fnu4HI GCNGC 4 cut(s) 192, 708, 745, 1951
FokI GGATG 3 cut(s) 29, 1026, 3346
FriOI GRGCYC 4 cut(s) 135, 2601, 2867, 3488
Fsp4HI GCNGC 4 cut(s) 192, 708, 745, 1951
FspBI CTAG 5 cut(s) 246, 1373, 1925, 2289, 2898
FspI TGCGCA 1 cut(s) 920
GlaI GCGC 4 cut(s) 416, 459, 920, 1162
GluI GCNGC 4 cut(s) 192, 708, 745, 1951
GsuI CTGGAG 5 cut(s) 1725, 2084, 2597, 2855, 3363
HaeII RGCGCY 2 cut(s) 418, 461
HaeIII GGCC 3 cut(s) 125, 2025, 2865
HapII CCGG 1 cut(s) 49
HgaI GACGC 4 cut(s) 326, 1673, 2825, 3045
HhaI GCGC 4 cut(s) 417, 460, 921, 1163
Hin1I GRCGYC 3 cut(s) 1645, 1684, 3037
Hin6I GCGC 4 cut(s) 415, 458, 919, 1161
HinP1I GCGC 4 cut(s) 415, 458, 919, 1161
HincII GTYRAC 3 cut(s) 994, 1688, 1759
HindII GTYRAC 3 cut(s) 994, 1688, 1759
HindIII AAGCTT 8 cut(s) 393, 1050, 2174, 2237, 2416, 2422, 2546, 2728
HpaI GTTAAC 1 cut(s) 994
HpaII CCGG 1 cut(s) 49
HphI GGTGA 4 cut(s) 1446, 1693, 2310, 2468
Hpy166II GTNNAC 8 cut(s) 54, 883, 994, 1688, 1759, 2736, 3111, 3381
Hpy8I GTNNAC 8 cut(s) 54, 883, 994, 1688, 1759, 2736, 3111, 3381
Hpy99I CGWCG 5 cut(s) 1647, 1650, 1689, 2819, 3039
HpyAV CCTTC 8 cut(s) 48, 366, 812, 1271, 1510, 1963, 2854, 3499
HpyCH4III ACNGT 7 cut(s) 12, 504, 1312, 1339, 1525, 1561, 2790
HpyCH4IV ACGT 7 cut(s) 94, 149, 301, 324, 1645, 2404, 3217
HpyF10VI GCNNNNNNNGC 7 cut(s) 188, 584, 707, 1414, 1873, 2596, 2898
HpySE526I ACGT 7 cut(s) 94, 149, 301, 324, 1645, 2404, 3217
Hsp92I GRCGYC 3 cut(s) 1645, 1684, 3037
HspAI GCGC 4 cut(s) 415, 458, 919, 1161
Ksp22I TGATCA 4 cut(s) 1240, 2772, 2832, 3529
KspAI GTTAAC 1 cut(s) 994
LguI GCTCTTC 2 cut(s) 405, 448
LmnI GCTCC 5 cut(s) 130, 179, 583, 1014, 2604
Lsp1109I GCAGC 4 cut(s) 203, 694, 756, 1962
LweI GCATC 8 cut(s) 7, 829, 1417, 1486, 1578, 1584, 1754, 1776
MaeI CTAG 5 cut(s) 246, 1373, 1925, 2289, 2898
MaeII ACGT 7 cut(s) 94, 149, 301, 324, 1645, 2404, 3217
MaeIII GTNAC 4 cut(s) 198, 1631, 2274, 3368
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 5 cut(s) 352, 475, 1121, 2534, 3236
MhlI GDGCHC 6 cut(s) 8, 135, 2601, 2867, 3421, 3488
MlyI GAGTC 5 cut(s) 227, 1220, 1613, 1732, 2046
MmeI TCCRAC 2 cut(s) 2204, 3310
Mph1103I ATGCAT 2 cut(s) 67, 3019
MroXI GAANNNNTTC 2 cut(s) 1672, 1845
MslI CAYNNNNRTG 5 cut(s) 265, 1494, 2019, 2768, 3513
MspCI CTTAAG 1 cut(s) 2419
MspI CCGG 1 cut(s) 49
MspR9I CCNGG 7 cut(s) 936, 1467, 1705, 2063, 2454, 2873, 3446
MunI CAATTG 1 cut(s) 588
Mva1269I GAATGC 5 cut(s) 65, 921, 1775, 2592, 3135
MvaI CCWGG 7 cut(s) 936, 1467, 1705, 2063, 2454, 2873, 3446
MvnI CGCG 1 cut(s) 1446
MwoI GCNNNNNNNGC 7 cut(s) 188, 584, 707, 1414, 1873, 2596, 2898
NlaIV GGNNCC 6 cut(s) 1464, 1975, 2504, 2865, 2866, 3238
NmeAIII GCCGAG 1 cut(s) 469
NmuCI GTSAC 1 cut(s) 2274
NruI TCGCGA 1 cut(s) 1446
NsbI TGCGCA 1 cut(s) 920
NsiI ATGCAT 2 cut(s) 67, 3019
NspI RCATGY 5 cut(s) 625, 1275, 1546, 3021, 3160
PaeI GCATGC 1 cut(s) 1275
PagI TCATGA 1 cut(s) 1243
PasI CCCWGGG 1 cut(s) 1466
PciI ACATGT 2 cut(s) 621, 3156
PciSI GCTCTTC 2 cut(s) 405, 448
PcsI WCGNNNNNNNCGW 1 cut(s) 2410
PctI GAATGC 5 cut(s) 65, 921, 1775, 2592, 3135
PdmI GAANNNNTTC 2 cut(s) 1672, 1845
PflFI GACNNNGTC 2 cut(s) 1683, 1692
PflMI CCANNNNNTGG 1 cut(s) 3047
PfoI TCCNGGA 1 cut(s) 934
PinAI ACCGGT 1 cut(s) 48
PkrI GCNGC 4 cut(s) 193, 709, 746, 1952
PleI GAGTC 5 cut(s) 226, 1220, 1612, 1732, 2046
PmaCI CACGTG 1 cut(s) 325
PmlI CACGTG 1 cut(s) 325
PpsI GAGTC 5 cut(s) 226, 1220, 1612, 1732, 2046
Ppu21I YACGTR 3 cut(s) 95, 325, 3218
PpuMI RGGWCCY 1 cut(s) 1462
PscI ACATGT 2 cut(s) 621, 3156
PshBI ATTAAT 1 cut(s) 1383
PsiI TTATAA 1 cut(s) 714
Psp124BI GAGCTC 2 cut(s) 135, 2601
Psp5II RGGWCCY 1 cut(s) 1462
Psp6I CCWGG 7 cut(s) 934, 1465, 1703, 2061, 2452, 2871, 3444
PspCI CACGTG 1 cut(s) 325
PspGI CCWGG 7 cut(s) 934, 1465, 1703, 2061, 2452, 2871, 3444
PspN4I GGNNCC 6 cut(s) 1464, 1975, 2504, 2865, 2866, 3238
PspOMI GGGCCC 1 cut(s) 2863
PspPI GGNCC 6 cut(s) 123, 497, 1462, 2863, 2864, 3111
PspPPI RGGWCCY 1 cut(s) 1462
PsuI RGATCY 5 cut(s) 352, 475, 1121, 2534, 3236
PsyI GACNNNGTC 2 cut(s) 1683, 1692
RruI TCGCGA 1 cut(s) 1446
RsaI GTAC 7 cut(s) 55, 1314, 1553, 1821, 2355, 2979, 3216
RsaNI GTAC 7 cut(s) 54, 1313, 1552, 1820, 2354, 2978, 3215
RseI CAYNNNNRTG 5 cut(s) 265, 1494, 2019, 2768, 3513
SacI GAGCTC 2 cut(s) 135, 2601
SalI GTCGAC 1 cut(s) 1686
SapI GCTCTTC 2 cut(s) 405, 448
SatI GCNGC 4 cut(s) 192, 708, 745, 1951
Sau96I GGNCC 6 cut(s) 123, 497, 1462, 2863, 2864, 3111
ScaI AGTACT 1 cut(s) 1314
SchI GAGTC 5 cut(s) 227, 1220, 1613, 1732, 2046
ScrFI CCNGG 7 cut(s) 936, 1467, 1705, 2063, 2454, 2873, 3446
SduI GDGCHC 6 cut(s) 8, 135, 2601, 2867, 3421, 3488
SfaNI GCATC 8 cut(s) 7, 829, 1417, 1486, 1578, 1584, 1754, 1776
SinI GGWCC 3 cut(s) 497, 1462, 3111
SmiMI CAYNNNNRTG 5 cut(s) 265, 1494, 2019, 2768, 3513
SmlI CTYRAG 5 cut(s) 70, 390, 2419, 2549, 3364
SmoI CTYRAG 5 cut(s) 70, 390, 2419, 2549, 3364
SphI GCATGC 1 cut(s) 1275
SsiI CCGC 2 cut(s) 1942, 3178
SspI AATATT 1 cut(s) 1507
SspMI CTAG 5 cut(s) 246, 1373, 1925, 2289, 2898
SstI GAGCTC 2 cut(s) 135, 2601
StyD4I CCNGG 7 cut(s) 934, 1465, 1703, 2061, 2452, 2871, 3444
StyI CCWWGG 2 cut(s) 126, 3096
TaaI ACNGT 7 cut(s) 12, 504, 1312, 1339, 1525, 1561, 2790
TaiI ACGT 7 cut(s) 97, 152, 304, 327, 1648, 2407, 3220
TaqI TCGA 7 cut(s) 171, 221, 1420, 1687, 1895, 2114, 3004
TatI WGTACW 3 cut(s) 53, 1312, 1819
TscAI CASTG 4 cut(s) 342, 1344, 2764, 3360
TseFI GTSAC 1 cut(s) 2274
TseI GCWGC 4 cut(s) 191, 707, 744, 1950
Tsp45I GTSAC 1 cut(s) 2274
TspGWI ACGGA 2 cut(s) 570, 2928
TspRI CASTG 4 cut(s) 342, 1344, 2764, 3360
Tth111I GACNNNGTC 2 cut(s) 1683, 1692
Van91I CCANNNNNTGG 1 cut(s) 3047
Vha464I CTTAAG 1 cut(s) 2419
VpaK11BI GGWCC 3 cut(s) 497, 1462, 3111
VspI ATTAAT 1 cut(s) 1383
XbaI TCTAGA 1 cut(s) 245
XceI RCATGY 5 cut(s) 625, 1275, 1546, 3021, 3160
XcmI CCANNNNNNNNNTGG 1 cut(s) 3179
XmiI GTMKAC 1 cut(s) 1687
XmnI GAANNNNTTC 2 cut(s) 1672, 1845
XspI CTAG 5 cut(s) 246, 1373, 1925, 2289, 2898
ZraI GACGTC 1 cut(s) 1646
ZrmI AGTACT 1 cut(s) 1314
Zsp2I ATGCAT 2 cut(s) 67, 3019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.