MD17G1276600.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000654
Physical Location & Seq
Forward (+)
33709205 .. 33713365
4161 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1276600.v1.1.491

Sequence Viewer

Length: 3492 bp
ATGGCTGCTAAAAGTTCCCGAGATTCTTCTTCGTCCTCTTACCGGTGTTCCGCCTTCTTGAGTTTCAGAGGCAAGGACACGCGCAAGGGGTTTACTGATCACCTCTATAGAGCCTTGGAGCTAGCAGGAATCCACACGTTTAGAGACGATGATGAAATCAAGAGAGGGGAGAATATTGAGTCGGAGTTAGAGAAGGCGATACAGGAGTCACAAGTATCCATAATTGTCTTCTCCAAGGACTACGCTTCCTCAAGGTGGTGTTTGAACGAACTTCTGAAGATCGTCGAACGTAGAAATACTGATCGTAGACATTTGGTTCTGCCAGTTTTCTATGATGTGGATCCATCCGATGTCAGGAAGCAGAGTGGTTCTTTTGCTGAAGCATTTGCCAGACATGAGGAGCGATTCAGTACGGAGATGGACAAGGTGGAGCTGTGGAGAAGAGCTCTTGGAGATGTTGCATCTTTGGGAGGCATGGTTTTAGGAGATCGGTATGAGGGGCAGTTCATCGAAGATATTGTTGAAGAGATTAGAAATAAAGTGGGTCACGCAGCTTTAGACGTCGCTCCCTTTGCAGTTGGAATGGATAATCGTGTGAGACGCCTAAACATGTGGTTACAAGATGGATCAAATGATGTTGGTTTAGCTGTCATCTGTGGGATGGGTGGAATTGGCAAGAGCACCATTGCGAAAGCTGCTTATAATCAAAACTTTGATAGATTTGAAGGTAGCAGCTTTCTTGCAGATATTCGAGAATCTTCAGAACAACCTAATGGTTTTGCTTGCTTGCAAAGAAAACTTCTTTCAGATATCCAAAAGGGGAACACAAAGAAAGTATACAATGTGGCTGAAGGAATAAGCAGGATCAAACGAGCTGTAGGCTACAAAAAAGTTCTTATTGTTCTCGATGATGTGAACAGCCGGGATCAACTCAATGCAATTCTTGGAATGCGAGAATGGCTTCATCAAGGAAGTAAAATTATCATAACAACTCGACATGAACATTTGTTAAATGCTCATGAAGTTTGTGAAAAGTTTATGGTTCGAGAACTGGATGAGTATGAATCACTTGAGCTTTTCAGTTGGCATGCCTTCAGACAATCCCATCCAGTAGAAGGTTACATGGAGCTTTCAAGACATGTGGTGCGGCACTGTGGAGGGCTTCCATTAGCTCTTCAAGTTGTGGGATCTTCTCTATCTGGAAAAAGTGAAGAAGTATGGCAAAGTGCATTGCAGAAGTTAGACGTGATCCCCAATGACAAAATTCAAAAAGTTCTGAGGATAAGTTTTGATTCTTTACCGGATGATCATGACAAAAATTTATTCCTTCACATAGCCAGCTTCTTCACAGGAAAGACGATGGATTATACAATTACAATACTGGACAACTTAGAGTTTTGCACAAGGATTGGGATTGAAAATCTAGTTGACAGATGCCTAGTGAAAATTCAAGGGTCGAAGTTGGCCATGCATCAGTTGGTGAGAAACATGGCAATGACAATTATTCGCGAAGAATCACCTCATGACCCTGGAAAACGTAGTAGAGTGTTGCAGAATGATGCCTCTAATATTTTGAGAAAATTAAGTGGCACGGAAAATATTAAGGGCCTCATGCTCAACCTTGCTAGCAAAAGAACATTTGTTGGAAGTGATAACAAACGATGCCATGTTGAAGATTATGATGGAAATTGTTCAAGACGACGTGGGCTTGGTTTCTTCTCTTGGAAGCCAATTAGCTTTTCCTCCACAAACTCAGCTTCTGCATCAAATGAGATAGATTTCAAATCTGAGGCGTTTAGAAGGATGCACAATCTTGAAATTCTCATTCTCAACAATGTCAACATCAGGGGAAGCTATGAAGAATTTTCAAAAAATTTAGTATGTTTATCTTGGCGAGGATTCCACTTAAAATCAATACCAAAAAATTTTTGTTTGGAAAACCTGGTTGTTCTAGACATGCGAAATAGTAGCCTACAACATGTTTGGAATGGGACCAGGGTTATTCCAAGGCTGAAGATTCTTAATTTCAGTTATTCGCATGGCCTTAGGACAATCCCCGACTTCTCAGGACTCTATAACCTTGAGAGGTTGATTCTTAAACATTGCATAAATTTGGTTGAGGTTCATGAGTCCATCGGACACCTAGAAAAACTTCTTGTTTTGAATCTAAAAGGCTGCAAAAATCTCATGAAGCTTCCAATATTGAGATCTATTCAGGTTCTTATTCTTTCCGGTTGCTCAAAGCTTGAATTGAACAAACTCAATCTGGTATCAGCTCAATCATGGTATTCAGTCTGGTCGAGGGTGTCGCCAAGAAAAAATACTGAATTAAGCAGTTTCTCATTGGCAAGTTTACCACATTCTTTGACTAGTTTAAGTCTGGCTTGCTGCAATATATCAGAGATTCCAAGTGCCCTAACTACGCTGTCCTCGTTAGAGTACTTGTCTCTGGATGAGAATCCGATTACAAGCCTACCAGAAAGCATGAACAATCTTGTTAAGCTCCAGGCTCTTAAAGTATCTGGTTGCAGAAACCTCACAATGCTTCCAGAGCTCCCACATGGTTTGATACAATTGAATGGTAGAGGTTGCACATCATTGAAAAGAATAACAAATTTAATGACCTTGGCCTTGTTACGGGATGACTTCCTTTCTGACATATTATTGGACCTACATCTATGGTATCGTGGGAAACTTGTTGATGTTGAAAGCTTGATGGATACACTAGCATTGGCCTCAGATCCTTCAGATCTATTCGATTTGCAACCCAAAGGTGGCATTGAGGTCCAAAGGAACTATTGTGAGAGTTTTGGCAGGAGAAGGCACATCCAGGGAACTTATGAATGCGGCATGTATAGCATATTTATTCATGGGAGCAAGATTCCAGATCGGTTCAATTACAGGAGCATGGGTAACACTGTGTTCTCTATTATTGTTCCTCCACATCTTAATCTCAAGATCGTAGGTTTAAACGCATGTATATTATATGCACGGCAGTCGGATAGGAGCCGTGAGGTTGCACGTGGCCGTCATTTCCATTGGCTTGAACTTAGAAATGAGACAAAGGGCGTTAAGTGGAAATGCCGTTTGTTTGATCAGGTTGAATGGAAAGTGATCAATGAAGATGCGTTATGGCTTAGCCATTGGATCATGGGGAACAATGAATTGGAATGCGGGGAAAAGGTGTGTTTTGAAATCAAAGAGCAATATGGTTTCCTCGCAAAGGAGATTGGAGTCCAGTTTGTGTACGAGCAGCAAAATAAGGATAAGGAGGATGTGCCATCAAGTAGTGAAGATACAACGATCCAACGTGAAAGATCTCCTTGGGAGTGGACCCGTAGTTTCCTTGACACCACAAGACCCCGTGTTCCGGTGGGTGTAGATACGGGGGTTCAATGCATCCATAGCTGCGATAAGAAAGTTATTTCAACTTTCATATGCGCGGCTGAATTCTATCTCGGTGACACTCTTAATGGAGCTGTTATTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1164

Amino Acids

132.24

Weight (kDa)

8.2

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 183 1.3e-48 TIR domain
TIR_2 PF13676 19 - 116 4.3e-17 TIR domain
NB-ARC PF00931 197 - 367 1.4e-19 NB-ARC domain
WHD_ROQ1 PF23282 436 - 506 3.4e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_8 PF13855 788 - 844 2.1e-08 Leucine rich repeat
LRR_14 PF23598 789 - 879 1.5e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 702
AatII GACGTC 1 cut(s) 564
AccI GTMKAC 2 cut(s) 307, 837
AccII CGCG 3 cut(s) 82, 1509, 3443
AciI CCGC 5 cut(s) 51, 1147, 2847, 3174, 3443
AcoI YGGCCR 2 cut(s) 1464, 3025
AcuI CTGAAG 7 cut(s) 296, 399, 744, 870, 1078, 2033, 2730
AcvI CACGTG 1 cut(s) 3023
AcyI GRCGYC 2 cut(s) 561, 601
AfaI GTAC 3 cut(s) 412, 2441, 3248
AfiI CCNNNNNNNGG 8 cut(s) 42, 255, 354, 1115, 2637, 2774, 3223, 3370
AflIII ACRYGT 4 cut(s) 135, 609, 1138, 1978
AgeI ACCGGT 1 cut(s) 42
AhlI ACTAGT 1 cut(s) 2369
AjiI CACGTC 2 cut(s) 1246, 1703
AjnI CCWGG 5 cut(s) 1528, 1941, 1994, 2505, 2829
AjuI GAANNNNNNNTTGG 4 cut(s) 1723, 1755, 3149, 3181
Alw21I GWGCWC 3 cut(s) 448, 683, 2556
Alw26I GTCTC 4 cut(s) 138, 592, 2451, 3053
AlwNI CAGNNNCTG 1 cut(s) 1760
Ama87I CYCGRG 1 cut(s) 18
AoxI GGCC 6 cut(s) 1464, 1606, 2041, 2628, 2733, 3025
ApeKI GCWGC 8 cut(s) 5, 551, 695, 732, 2175, 2388, 3253, 3408
AsiGI ACCGGT 1 cut(s) 42
Asp700I GAANNNNTTC 3 cut(s) 960, 1690, 2151
AspLEI GCGC 2 cut(s) 84, 3443
AspS9I GGNCC 5 cut(s) 1606, 1992, 2668, 2785, 3333
AsuC2I CCSGG 1 cut(s) 923
AsuHPI GGTGA 4 cut(s) 92, 1492, 1509, 3473
AsuNHI GCTAGC 2 cut(s) 121, 1625
AvaI CYCGRG 1 cut(s) 18
AvaII GGWCC 4 cut(s) 1992, 2668, 2785, 3333
AxyI CCTNAGG 1 cut(s) 2045
BaeGI GKGCMC 1 cut(s) 2416
BaeI ACNNNNGTAYC 4 cut(s) 191, 224, 3375, 3408
BalI TGGCCA 1 cut(s) 1466
BamHI GGATCC 1 cut(s) 340
BanII GRGCYC 2 cut(s) 448, 2556
BbrPI CACGTG 1 cut(s) 3023
BbsI GAAGAC 1 cut(s) 220
Bbv12I GWGCWC 3 cut(s) 448, 683, 2556
BbvI GCAGC 7 cut(s) 563, 682, 744, 2162, 2375, 3265, 3395
BceAI ACGGC 4 cut(s) 2994, 3008, 3012, 3069
BcgI CGANNNNNNTGC 2 cut(s) 2979, 3013
BciT130I CCWGG 5 cut(s) 1530, 1943, 1996, 2507, 2831
BciVI GTATCC 2 cut(s) 226, 2713
BclI TGATCA 4 cut(s) 97, 1306, 3094, 3114
BcnI CCSGG 1 cut(s) 923
BcoDI GTCTC 4 cut(s) 138, 592, 2451, 3053
BcuI ACTAGT 1 cut(s) 2369
BfaI CTAG 8 cut(s) 122, 1424, 1439, 1626, 1952, 2144, 2370, 2726
BfmI CTRYAG 2 cut(s) 106, 876
BfuI GTATCC 2 cut(s) 226, 2713
BglII AGATCT 3 cut(s) 2207, 2749, 3317
BlpI GCTNAGC 1 cut(s) 3137
BmcAI AGTACT 1 cut(s) 2441
Bme1390I CCNGG 6 cut(s) 923, 1530, 1943, 1996, 2507, 2831
Bme18I GGWCC 4 cut(s) 1992, 2668, 2785, 3333
BmeT110I CYCGRG 1 cut(s) 18
BmgBI CACGTC 2 cut(s) 1246, 1703
BmgT120I GGNCC 5 cut(s) 1606, 1992, 2668, 2785, 3333
BmiI GGNNCC 4 cut(s) 342, 1993, 3008, 3335
BmrFI CCNGG 6 cut(s) 923, 1530, 1943, 1996, 2507, 2831
BmsI GCATC 9 cut(s) 470, 1424, 1480, 1549, 1652, 1772, 1794, 3115, 3408
BmtI GCTAGC 2 cut(s) 125, 1629
BpiI GAAGAC 1 cut(s) 220
BplI GAGNNNNNCTC 2 cut(s) 430, 462
BpmI CTGGAG 1 cut(s) 2489
Bpu1102I GCTNAGC 1 cut(s) 3137
BpuEI CTTGAG 5 cut(s) 79, 235, 1091, 2102, 2939
BpuMI CCSGG 1 cut(s) 923
BsaAI YACGTR 1 cut(s) 3023
BsaBI GATNNNNATC 2 cut(s) 339, 2457
BsaHI GRCGYC 2 cut(s) 561, 601
BsaJI CCNNGG 8 cut(s) 114, 234, 1528, 1995, 2006, 2625, 2830, 3323
BsaWI WCCGGW 4 cut(s) 42, 1300, 2231, 3370
BsaXI ACNNNNNCTCC 4 cut(s) 462, 492, 2896, 2926
Bsc4I CCNNNNNNNGG 8 cut(s) 42, 255, 354, 1115, 2637, 2774, 3223, 3370
Bse118I RCCGGY 1 cut(s) 42
Bse1I ACTGG 5 cut(s) 323, 1056, 1109, 1386, 3238
Bse21I CCTNAGG 1 cut(s) 2045
Bse3DI GCAATG 4 cut(s) 684, 1229, 1500, 2101
Bse8I GATNNNNATC 2 cut(s) 339, 2457
BseBI CCWGG 5 cut(s) 1530, 1943, 1996, 2507, 2831
BseDI CCNNGG 8 cut(s) 114, 234, 1528, 1995, 2006, 2625, 2830, 3323
BseJI GATNNNNATC 2 cut(s) 339, 2457
BseLI CCNNNNNNNGG 8 cut(s) 42, 255, 354, 1115, 2637, 2774, 3223, 3370
BseMI GCAATG 4 cut(s) 684, 1229, 1500, 2101
BseMII CTCAG 5 cut(s) 1268, 1767, 1779, 2079, 2751
BseNI ACTGG 5 cut(s) 323, 1056, 1109, 1386, 3238
BseRI GAGGAG 1 cut(s) 413
BseSI GKGCMC 1 cut(s) 2416
BseXI GCAGC 7 cut(s) 563, 682, 744, 2162, 2375, 3265, 3395
Bsh1236I CGCG 3 cut(s) 82, 1509, 3443
BshFI GGCC 6 cut(s) 1466, 1608, 2043, 2630, 2735, 3027
BshTI ACCGGT 1 cut(s) 42
BsiHKAI GWGCWC 3 cut(s) 448, 683, 2556
BsiHKCI CYCGRG 1 cut(s) 18
BsiSI CCGG 5 cut(s) 43, 922, 1301, 2232, 3371
BslFI GGGAC 1 cut(s) 2005
BslI CCNNNNNNNGG 8 cut(s) 42, 255, 354, 1115, 2637, 2774, 3223, 3370
BsmAI GTCTC 4 cut(s) 138, 592, 2451, 3053
BsmBI CGTCTC 2 cut(s) 138, 592
BsmFI GGGAC 1 cut(s) 2005
BsmI GAATGC 3 cut(s) 954, 2849, 3176
BsnI GGCC 6 cut(s) 1466, 1608, 2043, 2630, 2735, 3027
BsoBI CYCGRG 1 cut(s) 18
Bsp1286I GDGCHC 4 cut(s) 448, 683, 2416, 2556
Bsp1720I GCTNAGC 1 cut(s) 3137
Bsp68I TCGCGA 1 cut(s) 1509
BspACI CCGC 5 cut(s) 51, 1147, 2847, 3174, 3443
BspANI GGCC 6 cut(s) 1466, 1608, 2043, 2630, 2735, 3027
BspCNI CTCAG 5 cut(s) 1269, 1766, 1780, 2078, 2750
BspFNI CGCG 3 cut(s) 82, 1509, 3443
BspHI TCATGA 5 cut(s) 1018, 1309, 1522, 2125, 2187
BspLI GGNNCC 4 cut(s) 342, 1993, 3008, 3335
BspOI GCTAGC 2 cut(s) 125, 1629
BspQI GCTCTTC 2 cut(s) 436, 1179
BsrDI GCAATG 4 cut(s) 684, 1229, 1500, 2101
BsrFI RCCGGY 1 cut(s) 42
BsrI ACTGG 5 cut(s) 323, 1056, 1109, 1386, 3238
BssAI RCCGGY 1 cut(s) 42
BssECI CCNNGG 8 cut(s) 114, 234, 1528, 1995, 2006, 2625, 2830, 3323
BssNAI GTATAC 1 cut(s) 838
BssNI GRCGYC 2 cut(s) 561, 601
BssT1I CCWWGG 5 cut(s) 114, 234, 2006, 2625, 3323
Bst1107I GTATAC 1 cut(s) 838
Bst2UI CCWGG 5 cut(s) 1530, 1943, 1996, 2507, 2831
Bst4CI ACNGT 2 cut(s) 1154, 2920
Bst6I CTCTTC 3 cut(s) 436, 519, 1179
BstACI GRCGYC 2 cut(s) 561, 601
BstBAI YACGTR 1 cut(s) 3023
BstC8I GCNNGC 7 cut(s) 123, 784, 788, 1089, 1339, 1627, 2386
BstDEI CTNAG 9 cut(s) 1277, 1390, 1753, 1788, 2045, 2065, 2737, 3050, 3137
BstENI CCTNNNNNAGG 1 cut(s) 3221
BstFNI CGCG 3 cut(s) 82, 1509, 3443
BstHHI GCGC 2 cut(s) 84, 3443
BstMAI GTCTC 4 cut(s) 138, 592, 2451, 3053
BstMWI GCNNNNNNNGC 6 cut(s) 572, 695, 958, 2551, 2856, 3405
BstNI CCWGG 5 cut(s) 1530, 1943, 1996, 2507, 2831
BstNSI RCATGY 7 cut(s) 613, 1091, 1142, 1960, 1982, 2854, 2979
BstSCI CCNGG 6 cut(s) 921, 1528, 1941, 1994, 2505, 2829
BstSFI CTRYAG 2 cut(s) 106, 876
BstSLI GKGCMC 1 cut(s) 2416
BstUI CGCG 3 cut(s) 82, 1509, 3443
BstV1I GCAGC 7 cut(s) 563, 682, 744, 2162, 2375, 3265, 3395
BstV2I GAAGAC 1 cut(s) 220
BstX2I RGATCY 6 cut(s) 340, 1187, 2207, 2740, 2749, 3317
BstYI RGATCY 6 cut(s) 340, 1187, 2207, 2740, 2749, 3317
BstZ17I GTATAC 1 cut(s) 838
Bsu36I CCTNAGG 1 cut(s) 2045
BsuI GTATCC 2 cut(s) 226, 2713
BsuRI GGCC 6 cut(s) 1466, 1608, 2043, 2630, 2735, 3027
BtrI CACGTC 2 cut(s) 1246, 1703
BtsIMutI CAGTG 2 cut(s) 1150, 2916
BtuMI TCGCGA 1 cut(s) 1509
Cac8I GCNNGC 7 cut(s) 123, 784, 788, 1089, 1339, 1627, 2386
CaiI CAGNNNCTG 1 cut(s) 1760
CciI TCATGA 5 cut(s) 1018, 1309, 1522, 2125, 2187
CfoI GCGC 2 cut(s) 84, 3443
Cfr10I RCCGGY 1 cut(s) 42
Cfr13I GGNCC 5 cut(s) 1606, 1992, 2668, 2785, 3333
CseI GACGC 1 cut(s) 609
CsiI ACCWGGT 1 cut(s) 1941
Csp6I GTAC 3 cut(s) 411, 2440, 3247
CspAI ACCGGT 1 cut(s) 42
CspCI CAANNNNNGTGG 6 cut(s) 1122, 1157, 2346, 2381, 2547, 2582
CviQI GTAC 3 cut(s) 411, 2440, 3247
DdeI CTNAG 9 cut(s) 1277, 1390, 1753, 1788, 2045, 2065, 2737, 3050, 3137
DraI TTTAAA 1 cut(s) 2970
EaeI YGGCCR 2 cut(s) 1464, 3025
Eam1104I CTCTTC 3 cut(s) 436, 519, 1179
EarI CTCTTC 3 cut(s) 436, 519, 1179
EciI GGCGGA 1 cut(s) 40
Ecl136II GAGCTC 2 cut(s) 446, 2554
Eco130I CCWWGG 5 cut(s) 114, 234, 2006, 2625, 3323
Eco24I GRGCYC 2 cut(s) 448, 2556
Eco32I GATATC 1 cut(s) 811
Eco47I GGWCC 4 cut(s) 1992, 2668, 2785, 3333
Eco53kI GAGCTC 2 cut(s) 446, 2554
Eco57I CTGAAG 7 cut(s) 296, 399, 744, 870, 1078, 2033, 2730
Eco72I CACGTG 1 cut(s) 3023
Eco81I CCTNAGG 1 cut(s) 2045
Eco88I CYCGRG 1 cut(s) 18
EcoICRI GAGCTC 2 cut(s) 446, 2554
EcoNI CCTNNNNNAGG 1 cut(s) 3221
EcoO109I RGGNCCY 1 cut(s) 1606
EcoRI GAATTC 1 cut(s) 3449
EcoRII CCWGG 5 cut(s) 1528, 1941, 1994, 2505, 2829
EcoRV GATATC 1 cut(s) 811
EcoT14I CCWWGG 5 cut(s) 114, 234, 2006, 2625, 3323
EcoT22I ATGCAT 2 cut(s) 1473, 3401
EcoT38I GRGCYC 2 cut(s) 448, 2556
ErhI CCWWGG 5 cut(s) 114, 234, 2006, 2625, 3323
Esp3I CGTCTC 2 cut(s) 138, 592
FalI AAGNNNNNCTT 4 cut(s) 2368, 2400, 3307, 3339
FaqI GGGAC 1 cut(s) 2005
FauI CCCGC 1 cut(s) 3167
FauNDI CATATG 1 cut(s) 3437
FbaI TGATCA 4 cut(s) 97, 1306, 3094, 3114
FblI GTMKAC 2 cut(s) 307, 837
FriOI GRGCYC 2 cut(s) 448, 2556
FspBI CTAG 8 cut(s) 122, 1424, 1439, 1626, 1952, 2144, 2370, 2726
GlaI GCGC 2 cut(s) 83, 3442
GsuI CTGGAG 1 cut(s) 2489
HaeIII GGCC 6 cut(s) 1466, 1608, 2043, 2630, 2735, 3027
HapII CCGG 5 cut(s) 43, 922, 1301, 2232, 3371
HgaI GACGC 1 cut(s) 609
HhaI GCGC 2 cut(s) 84, 3443
Hin1I GRCGYC 2 cut(s) 561, 601
Hin6I GCGC 2 cut(s) 82, 3441
HinP1I GCGC 2 cut(s) 82, 3441
HincII GTYRAC 2 cut(s) 1429, 1840
HindII GTYRAC 2 cut(s) 1429, 1840
HindIII AAGCTT 3 cut(s) 2192, 2243, 2710
HpaII CCGG 5 cut(s) 43, 922, 1301, 2232, 3371
HphI GGTGA 4 cut(s) 92, 1492, 1509, 3473
Hpy166II GTNNAC 9 cut(s) 93, 308, 838, 916, 1429, 1840, 2354, 3247, 3333
Hpy8I GTNNAC 9 cut(s) 93, 308, 838, 916, 1429, 1840, 2354, 3247, 3333
Hpy99I CGWCG 3 cut(s) 287, 566, 1704
HpyCH4III ACNGT 2 cut(s) 1154, 2920
HpyCH4IV ACGT 8 cut(s) 137, 289, 561, 1245, 1537, 1702, 3022, 3310
HpyF10VI GCNNNNNNNGC 6 cut(s) 572, 695, 958, 2551, 2856, 3405
HpyF3I CTNAG 9 cut(s) 1277, 1390, 1753, 1788, 2045, 2065, 2737, 3050, 3137
HpySE526I ACGT 8 cut(s) 137, 289, 561, 1245, 1537, 1702, 3022, 3310
Hsp92I GRCGYC 2 cut(s) 561, 601
HspAI GCGC 2 cut(s) 82, 3441
Ksp22I TGATCA 4 cut(s) 97, 1306, 3094, 3114
LguI GCTCTTC 2 cut(s) 436, 1179
Lsp1109I GCAGC 7 cut(s) 563, 682, 744, 2162, 2375, 3265, 3395
LweI GCATC 9 cut(s) 470, 1424, 1480, 1549, 1652, 1772, 1794, 3115, 3408
MabI ACCWGGT 1 cut(s) 1941
MaeI CTAG 8 cut(s) 122, 1424, 1439, 1626, 1952, 2144, 2370, 2726
MaeII ACGT 8 cut(s) 137, 289, 561, 1245, 1537, 1702, 3022, 3310
MaeIII GTNAC 7 cut(s) 207, 545, 615, 1118, 2634, 2912, 3461
MfeI CAATTG 1 cut(s) 2573
MflI RGATCY 6 cut(s) 340, 1187, 2207, 2740, 2749, 3317
MhlI GDGCHC 4 cut(s) 448, 683, 2416, 2556
MlsI TGGCCA 1 cut(s) 1466
MluNI TGGCCA 1 cut(s) 1466
MlyI GAGTC 5 cut(s) 188, 215, 2064, 2138, 3243
MmeI TCCRAC 5 cut(s) 162, 559, 1624, 2979, 3331
Mox20I TGGCCA 1 cut(s) 1466
Mph1103I ATGCAT 2 cut(s) 1473, 3401
MroXI GAANNNNTTC 3 cut(s) 960, 1690, 2151
MscI TGGCCA 1 cut(s) 1466
MslI CAYNNNNRTG 1 cut(s) 1493
Msp20I TGGCCA 1 cut(s) 1466
MspI CCGG 5 cut(s) 43, 922, 1301, 2232, 3371
MspR9I CCNGG 6 cut(s) 923, 1530, 1943, 1996, 2507, 2831
MssI GTTTAAAC 1 cut(s) 2970
MunI CAATTG 1 cut(s) 2573
Mva1269I GAATGC 3 cut(s) 954, 2849, 3176
MvaI CCWGG 5 cut(s) 1530, 1943, 1996, 2507, 2831
MvnI CGCG 3 cut(s) 82, 1509, 3443
MwoI GCNNNNNNNGC 6 cut(s) 572, 695, 958, 2551, 2856, 3405
NciI CCSGG 1 cut(s) 923
NdeI CATATG 1 cut(s) 3437
NheI GCTAGC 2 cut(s) 121, 1625
NlaIV GGNNCC 4 cut(s) 342, 1993, 3008, 3335
NmuCI GTSAC 3 cut(s) 207, 545, 3461
NruI TCGCGA 1 cut(s) 1509
NsiI ATGCAT 2 cut(s) 1473, 3401
NspI RCATGY 7 cut(s) 613, 1091, 1142, 1960, 1982, 2854, 2979
PaeI GCATGC 1 cut(s) 1091
PagI TCATGA 5 cut(s) 1018, 1309, 1522, 2125, 2187
PciI ACATGT 3 cut(s) 609, 1138, 1978
PciSI GCTCTTC 2 cut(s) 436, 1179
PcsI WCGNNNNNNNCGW 1 cut(s) 2429
PctI GAATGC 3 cut(s) 954, 2849, 3176
PdmI GAANNNNTTC 3 cut(s) 960, 1690, 2151
PinAI ACCGGT 1 cut(s) 42
PleI GAGTC 5 cut(s) 187, 214, 2064, 2137, 3242
PmaCI CACGTG 1 cut(s) 3023
PmeI GTTTAAAC 1 cut(s) 2970
PmlI CACGTG 1 cut(s) 3023
PpsI GAGTC 5 cut(s) 187, 214, 2064, 2137, 3242
Ppu21I YACGTR 1 cut(s) 3023
PscI ACATGT 3 cut(s) 609, 1138, 1978
PsiI TTATAA 1 cut(s) 702
Psp124BI GAGCTC 2 cut(s) 448, 2556
Psp6I CCWGG 5 cut(s) 1528, 1941, 1994, 2505, 2829
PspCI CACGTG 1 cut(s) 3023
PspGI CCWGG 5 cut(s) 1528, 1941, 1994, 2505, 2829
PspN4I GGNNCC 4 cut(s) 342, 1993, 3008, 3335
PspPI GGNCC 5 cut(s) 1606, 1992, 2668, 2785, 3333
PstNI CAGNNNCTG 1 cut(s) 1760
PsuI RGATCY 6 cut(s) 340, 1187, 2207, 2740, 2749, 3317
RruI TCGCGA 1 cut(s) 1509
RsaI GTAC 3 cut(s) 412, 2441, 3248
RsaNI GTAC 3 cut(s) 411, 2440, 3247
RseI CAYNNNNRTG 1 cut(s) 1493
SacI GAGCTC 2 cut(s) 448, 2556
SapI GCTCTTC 2 cut(s) 436, 1179
Sau96I GGNCC 5 cut(s) 1606, 1992, 2668, 2785, 3333
ScaI AGTACT 1 cut(s) 2441
SchI GAGTC 5 cut(s) 188, 215, 2064, 2138, 3243
ScrFI CCNGG 6 cut(s) 923, 1530, 1943, 1996, 2507, 2831
SduI GDGCHC 4 cut(s) 448, 683, 2416, 2556
SexAI ACCWGGT 1 cut(s) 1941
SfaNI GCATC 9 cut(s) 470, 1424, 1480, 1549, 1652, 1772, 1794, 3115, 3408
SfcI CTRYAG 2 cut(s) 106, 876
SinI GGWCC 4 cut(s) 1992, 2668, 2785, 3333
SmiMI CAYNNNNRTG 1 cut(s) 1493
SmlI CTYRAG 5 cut(s) 58, 250, 1070, 2081, 2954
SmoI CTYRAG 5 cut(s) 58, 250, 1070, 2081, 2954
SpeI ACTAGT 1 cut(s) 2369
SphI GCATGC 1 cut(s) 1091
SsiI CCGC 5 cut(s) 51, 1147, 2847, 3174, 3443
SspI AATATT 4 cut(s) 175, 1570, 1600, 2202
SspMI CTAG 8 cut(s) 122, 1424, 1439, 1626, 1952, 2144, 2370, 2726
SstI GAGCTC 2 cut(s) 448, 2556
StyD4I CCNGG 6 cut(s) 921, 1528, 1941, 1994, 2505, 2829
StyI CCWWGG 5 cut(s) 114, 234, 2006, 2625, 3323
TaaI ACNGT 2 cut(s) 1154, 2920
TaiI ACGT 8 cut(s) 140, 292, 564, 1248, 1540, 1705, 3025, 3313
TaqI TCGA 9 cut(s) 285, 510, 751, 906, 994, 1045, 1457, 2300, 2757
TatI WGTACW 1 cut(s) 2439
TauI GCSGC 3 cut(s) 1150, 2850, 3446
TscAI CASTG 2 cut(s) 1157, 2923
TseFI GTSAC 3 cut(s) 207, 545, 3461
TseI GCWGC 8 cut(s) 5, 551, 695, 732, 2175, 2388, 3253, 3408
Tsp45I GTSAC 3 cut(s) 207, 545, 3461
TspGWI ACGGA 2 cut(s) 428, 1607
TspRI CASTG 2 cut(s) 1157, 2923
VpaK11BI GGWCC 4 cut(s) 1992, 2668, 2785, 3333
XagI CCTNNNNNAGG 1 cut(s) 3221
XbaI TCTAGA 1 cut(s) 1951
XceI RCATGY 7 cut(s) 613, 1091, 1142, 1960, 1982, 2854, 2979
XcmI CCANNNNNNNNNTGG 1 cut(s) 1474
XmiI GTMKAC 2 cut(s) 307, 837
XmnI GAANNNNTTC 3 cut(s) 960, 1690, 2151
XspI CTAG 8 cut(s) 122, 1424, 1439, 1626, 1952, 2144, 2370, 2726
ZraI GACGTC 1 cut(s) 562
ZrmI AGTACT 1 cut(s) 2441
Zsp2I ATGCAT 2 cut(s) 1473, 3401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.