Rroxscaffold_4G00313580

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000654
Physical Location & Seq
Forward (+)
37081303 .. 37086062
4760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00313580.1

Sequence Viewer

Length: 3591 bp
ATGGCTGCTTCTCCATCCAGATGCCGCTATCATGCCTTCCTGAGTTTCAGGGGCACAGATACACGCAAGGGCTTTAGTGATCATCTCTATAGAGCTTTGGAGTTGGCTGGAATTCACACTTTCAGAGATGATGATGAAATTGAGAGAGGAGCAGAGATAGCAGTAGAGCTGCAGAAGGCTATCCAGGAATCACAGGTATCAATAATCGTGTTTTCCGAGAATTATGCTTCCTCAAGATGGTGCTTGGATGAGCTCGCCAATATCATGAAACGTAGAAGAAAAGATGGACACATGGTTTTGCCAGTTTTCTATAAGGTGCCCCCAACCAATGTTAGACATCAGACTGGCAGTTTTGAAGAAGCGTTTGCCAAACATGAAGAGCGCTTCAAGGAGGAGATGGGCAAGGTGCACGAGTGGAGAAGATCTCTTAAAGATGCTGCCGATCTGGGTGGGATGGAATTAGGAGATCAGTATGAATCACAGCTGATCAAAGATATTGTTGAGAAGATTGGAAATAAACTGGAACCAAAACTATTAAATCTACCTCCTTATGCGGTTGGAATAGATGATCGTGTGGAAGATATAAACATGTGGTTAAGAGAAGGATCAACTGATGTTGGTGTAGCTTGCATTTATGGAATGGGTGGAATCGGCAAGACCACCATTGCAAAAGCTGCTTATAACATGAACTGGGTTAACTTTCAAGGAAGTAGCTTTCTTGAAGATATTAGAGCAACTTCAGAACAACCTAATGGTTTGGTTCGCTTGCAAATGAAACTTATATCTGATATCCAGAATCAGAAGCCAAAGAAGATATACTCTGTTAATGAAGGAATGAGCCGGATCCAGCACGTTGTATGTTGCAAAAGAGTTCTTATTGTTCTTGATGATGTAGATGATTTGGACCAATTCAGGGCAACTCTTGGAATGACAGAATGGTTTCATCCTGGAAGTAAGATTATTATAACAACTAGACATTTACACTTGCTGAAAGCCAATGAGGTTTCTACAAGGTTCAAGGTGCAAGAACTTGATGAGCATGAATCACTTGAGCTTTTCTGTTGGCATGCTTTTCGACAAGCTCATCCTATTAAAAGCTACATGGAGGTTTCTACAGATGTAGTACAACATTGTGGAGGGCTTCCGTTAGCTATTCGAGTTTTGGGGTCTTCTCTGTATGGAGAAAGTGTAGATGTATGGCAAAGTGCATTAGAGAAGTTGGATGTGATTCCTCATCGCGATATCCAAAAGGTTCTAAGAGTAAGCTTTGATTCTCTGCAAGATGATTGCGACAAAAATTTGTTTCTTCATATATCCTGTTTCTTCATTGGAAAGGAGAAAAATTTTGCAATTACAGTACTGAACGACCTTGATCTTTTCCCAATAATTGGAATTAAAAATCTAGTCGATAGATGTTTAGTGCAAATTGATAACGACAGGTTGATCATGCATCAATTACTTATTGACATGGCAAGGGGAATTATTCGTGAAGAGTCCCCAGAGAACCCTGGAAAACGTAGTAGACTGTGGGATAAGGATGCATTTGATGTTTTGGATAACATAACAGGTACAGAAACTATTAAAGGTCTCATGCTCAACCTTCACAAGGAAAATACCCTTTTTAACAAAAAACGATGCCACACTGAAGAGTATGATAGAAATTGTTCCAGATGGCGTTGGTTTGGTTTATTCTCATGGAAATTAAACAACTTTTCCTCAACAGAGTTATGTTCAGATATGCCGAACTTCAAATCAGCGGCACTTAAAAGGATGCACGATCTTAAAATTCTTCTGCTCGATAATGTAAAATTCAGTGGTTGCTGTGACGACTTTCCAAGAAAAATATCATGGTTTTCTTGGCAAGGGTTTCCTTCAGAATCTATACCGAAGGAATTTCATTTGCAAACTCTAGTTGTTTTGGAGTTGCAACACAGCAAACTGCAAGTTGTTTGGAAGGGGAAAAAGAATCTGCCGAAATTAAAAATCCTTGATCTCAGTCATTCACTTGATCTTGTTACAACCCCCGACTTGTCAGGACTCTCTAGCCTTGAGAGATTAATCCTTGAAGGTTGCACGAACATTGCTGAGATTGATGAATCCATTGGTGATCTTGAGAAACTTGTTTCCTTGAATCTCAAATACTGCAAAAATCTCGAGAAGCTCCCAGGGACAATTGGCAGGTTGACATCTCTTCAGGAGCTTAGTCTTTCTGGTTGTTCAAACTTTGCTCTTCCTGCTAATACAGCTACTAATATGATGAATTTCTTCAGCCTGTTATCAAATAAATCCTGGAAATCAATTTGGTCGCATGTATCACCAAGAGAAGGTGTTGAACCAACCAGTTTGTCATTGATAAATTTGCAACGTTCTTTAGTACGGTTGAATCTGGCTGATTGCAATATATCAGAGATTCCCAATGATCTTAGTATCCTTTCCTCATTGAAGGAACTGGATCTAAGCTCTAATCCATTTATGAGTCTACCTGAAAACATGAAGAGTATTATTGTGCTCAAGTCTCTTTATATAGACTACTGCGAAAACCTCAAAATGCTTCCGGAGCTCCCACCAAGTTTATGTGTATTACAAGCAATGATGTGTACATCATTGAAAAAGGTAACAAATGTACCCGATATGCGGGGTCCACGGTTCTTCATTTTTTTTGCCGCTGGAGAATTAGTTGAAGTTGGAGGCGTGTTCAGTATAAAGCCATTGATCAGCGAAATTGACGTGGAAATGATAAGAAATATTGGCCTGTACAATTTGGAACCCTCTGGAGGAACTGAGGAGCTTGAAATGATAAATTTCTTGACGCTAACAAGCAGGACATGGCCCCTCCAGGGACTCTGTGAATGTGGCATATTCAGCATTTTCCTTCGTGGAAGCAATATTCCTGATTGGTACGAGTACAGAGGTGAAACCGCGTTGTCCGTCACTGTTCCTTCCCATCCTAAACTCAAGATTAAAGGCTTAAATGTCTGTGTTGTGTACACCCCAGATTGTGTTGTGAATGAAAATGGAGCTCGGATACTGGGTTCACTCATTCTAAAAGTCAGTAATGAAGCGAAGGGTCTTATGTGGTCATATTACCCTATCAGTGTAGGTCTTCCAAAAGTGAATGAAGAAATGTCATGGCTAAGCCATTGGCAGTTTGAGAATGATGAGTTAGAGGGCGGGGAAGAACTACGTTTTTCTGTGGATAGCAAATATTTCAGTTTTCTTATCGGTAGAGATTTTCGGCTCTTGATAAAGGAGCTTGCAGTCCAACTTGTGTACGAGAATGAAAATGAGGGCAAGGCATGTAATGGTATCCAATTATCAAATCCTGAAGATGTTACGACCCAACATGAAGCACCTTATTGGAGCCAGGGTGTCCTCATTGGACACGTATCGGCACCACAGTATCAAATGGTGTCAGGCAAATACTTCCTTTGCAATCTTTACGGATTTTCTCGAAATTTTCGTTCTTCAGTTCAAGAGCCCACAGATTTGAATAGTTTATTGGGAAAATCAATTGGGTTCCCTTTTCAGCACGAAGATTTTGAGAGACGTGTCTTGGGACAAAAAAATGAGGAACGAATTTTGCTTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

1196

Amino Acids

136.15

Weight (kDa)

5.84

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 10 - 177 3.5e-51 TIR domain
TIR_2 PF13676 13 - 106 3.8e-15 TIR domain
NB-ARC PF00931 189 - 360 5.7e-19 NB-ARC domain
WHD_ROQ1 PF23282 431 - 499 7.1e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 631 - 739 3.3e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 681, 965
Acc36I ACCTGC 1 cut(s) 2169
AccB1I GGYRCC 2 cut(s) 316, 3391
AccB7I CCANNNNNTGG 1 cut(s) 1388
AccI GTMKAC 2 cut(s) 1522, 2479
AccII CGCG 2 cut(s) 1239, 2921
AccIII TCCGGA 1 cut(s) 2554
AciI CCGC 7 cut(s) 25, 554, 1757, 2635, 2664, 2919, 3171
AclI AACGTT 1 cut(s) 2365
AclWI GGATC 4 cut(s) 613, 838, 851, 2460
AcuI CTGAAG 7 cut(s) 723, 1665, 1857, 2177, 2251, 3345, 3450
AfeI AGCGCT 1 cut(s) 383
AfiI CCNNNNNNNGG 8 cut(s) 237, 913, 1388, 1606, 2324, 2634, 2774, 2837
AflIII ACRYGT 3 cut(s) 588, 3382, 3547
AjiI CACGTC 2 cut(s) 2728, 3548
AjnI CCWGG 7 cut(s) 183, 946, 1507, 2164, 2288, 2835, 3363
AjuI GAANNNNNNNTTGG 2 cut(s) 3482, 3514
AloI GAACNNNNNNTCC 2 cut(s) 3016, 3048
Alw21I GWGCWC 5 cut(s) 255, 411, 2511, 2562, 3022
Alw26I GTCTC 3 cut(s) 1592, 2520, 3538
Alw44I GTGCAC 1 cut(s) 407
AlwI GGATC 4 cut(s) 613, 838, 851, 2460
Ama87I CYCGRG 1 cut(s) 2153
Aor13HI TCCGGA 1 cut(s) 2554
Aor51HI AGCGCT 1 cut(s) 383
AoxI GGCC 2 cut(s) 2749, 2828
ApaLI GTGCAC 1 cut(s) 407
ApeKI GCWGC 4 cut(s) 5, 169, 437, 674
AseI ATTAAT 1 cut(s) 2057
Asp700I GAANNNNTTC 3 cut(s) 939, 1663, 2264
AspLEI GCGC 1 cut(s) 384
AspS9I GGNCC 3 cut(s) 904, 2639, 2829
AsuHPI GGTGA 3 cut(s) 2117, 2307, 2924
AvaI CYCGRG 1 cut(s) 2153
AvaII GGWCC 2 cut(s) 904, 2639
BaeGI GKGCMC 3 cut(s) 56, 321, 411
BaeI ACNNNNGTAYC 4 cut(s) 2607, 2640, 3383, 3416
BamHI GGATCC 1 cut(s) 843
BanI GGYRCC 2 cut(s) 316, 3391
BanII GRGCYC 4 cut(s) 255, 2562, 3022, 3480
BauI CACGAG 1 cut(s) 410
BbsI GAAGAC 2 cut(s) 1161, 3095
Bbv12I GWGCWC 5 cut(s) 255, 411, 2511, 2562, 3022
BbvI GCAGC 3 cut(s) 156, 424, 661
BccI CCATC 7 cut(s) 22, 231, 278, 391, 448, 1665, 2952
BcgI CGANNNNNNTGC 6 cut(s) 206, 240, 1055, 1089, 2134, 2168
BciT130I CCWGG 7 cut(s) 185, 948, 1509, 2166, 2290, 2837, 3365
BciVI GTATCC 3 cut(s) 2438, 3018, 3317
BclI TGATCA 4 cut(s) 79, 486, 1443, 2712
BcoDI GTCTC 3 cut(s) 1592, 2520, 3538
BfaI CTAG 4 cut(s) 972, 1403, 1910, 2043
BfmI CTRYAG 3 cut(s) 88, 170, 1113
BfoI RGCGCY 1 cut(s) 385
BfuAI ACCTGC 1 cut(s) 2169
BfuI GTATCC 3 cut(s) 2438, 3018, 3317
BglII AGATCT 1 cut(s) 422
BisI GCNGC 7 cut(s) 6, 25, 170, 438, 675, 1758, 2664
BlpI GCTNAGC 1 cut(s) 3134
BlsI GCNGC 7 cut(s) 7, 26, 171, 439, 676, 1759, 2665
BmcAI AGTACT 1 cut(s) 1359
Bme1390I CCNGG 7 cut(s) 185, 948, 1509, 2166, 2290, 2837, 3365
Bme18I GGWCC 2 cut(s) 904, 2639
BmeT110I CYCGRG 1 cut(s) 2153
BmgBI CACGTC 2 cut(s) 2728, 3548
BmgT120I GGNCC 3 cut(s) 904, 2639, 2829
BmiI GGNNCC 9 cut(s) 318, 525, 845, 2640, 2766, 2831, 3362, 3393, 3518
BmrFI CCNGG 7 cut(s) 185, 948, 1509, 2166, 2290, 2837, 3365
BmrI ACTGGG 2 cut(s) 700, 3038
BmsI GCATC 6 cut(s) 11, 424, 1459, 1528, 1625, 1761
BmuI ACTGGG 2 cut(s) 700, 3038
BpiI GAAGAC 2 cut(s) 1161, 3095
BplI GAGNNNNNCTC 2 cut(s) 409, 441
BpmI CTGGAG 3 cut(s) 2688, 2793, 2819
Bpu1102I GCTNAGC 1 cut(s) 3134
BpuEI CTTGAG 6 cut(s) 217, 1070, 2069, 2132, 2495, 2939
BsaAI YACGTR 1 cut(s) 3385
BsaI GGTCTC 1 cut(s) 1592
BsaJI CCNNGG 6 cut(s) 1507, 2164, 2165, 2642, 2836, 3364
BsaWI WCCGGW 1 cut(s) 2554
BsaXI ACNNNNNCTCC 6 cut(s) 1173, 1203, 2661, 2691, 3352, 3382
Bsc4I CCNNNNNNNGG 8 cut(s) 237, 913, 1388, 1606, 2324, 2634, 2774, 2837
Bse1I ACTGG 7 cut(s) 302, 349, 525, 695, 2340, 2454, 3033
Bse3DI GCAATG 3 cut(s) 663, 2079, 2595
BseAI TCCGGA 1 cut(s) 2554
BseBI CCWGG 7 cut(s) 185, 948, 1509, 2166, 2290, 2837, 3365
BseDI CCNNGG 6 cut(s) 1507, 2164, 2165, 2642, 2836, 3364
BseGI GGATG 9 cut(s) 14, 253, 459, 943, 1084, 1228, 1543, 1776, 2944
BseLI CCNNNNNNNGG 8 cut(s) 237, 913, 1388, 1606, 2324, 2634, 2774, 2837
BseMI GCAATG 3 cut(s) 663, 2079, 2595
BseMII CTCAG 4 cut(s) 32, 2008, 2076, 2772
BseNI ACTGG 7 cut(s) 302, 349, 525, 695, 2340, 2454, 3033
BseRI GAGGAG 3 cut(s) 162, 407, 2798
BseSI GKGCMC 3 cut(s) 56, 321, 411
BseXI GCAGC 3 cut(s) 156, 424, 661
Bsh1236I CGCG 2 cut(s) 1239, 2921
BshFI GGCC 2 cut(s) 2751, 2830
BshNI GGYRCC 2 cut(s) 316, 3391
BsiHKAI GWGCWC 5 cut(s) 255, 411, 2511, 2562, 3022
BsiHKCI CYCGRG 1 cut(s) 2153
BsiSI CCGG 2 cut(s) 841, 2555
BslFI GGGAC 4 cut(s) 1480, 2182, 2853, 3570
BslI CCNNNNNNNGG 8 cut(s) 237, 913, 1388, 1606, 2324, 2634, 2774, 2837
BsmAI GTCTC 3 cut(s) 1592, 2520, 3538
BsmBI CGTCTC 1 cut(s) 3538
BsmFI GGGAC 4 cut(s) 1480, 2182, 2853, 3570
BsnI GGCC 2 cut(s) 2751, 2830
Bso31I GGTCTC 1 cut(s) 1592
BsoBI CYCGRG 1 cut(s) 2153
Bsp1286I GDGCHC 8 cut(s) 56, 255, 321, 411, 2511, 2562, 3022, 3480
Bsp13I TCCGGA 1 cut(s) 2554
Bsp1407I TGTACA 3 cut(s) 2597, 2754, 2985
Bsp1720I GCTNAGC 1 cut(s) 3134
Bsp68I TCGCGA 1 cut(s) 1239
BspACI CCGC 7 cut(s) 25, 554, 1757, 2635, 2664, 2919, 3171
BspANI GGCC 2 cut(s) 2751, 2830
BspCNI CTCAG 4 cut(s) 33, 2007, 2077, 2773
BspEI TCCGGA 1 cut(s) 2554
BspFNI CGCG 2 cut(s) 1239, 2921
BspHI TCATGA 1 cut(s) 264
BspLI GGNNCC 9 cut(s) 318, 525, 845, 2640, 2766, 2831, 3362, 3393, 3518
BspMAI CTGCAG 1 cut(s) 174
BspMI ACCTGC 1 cut(s) 2169
BspPI GGATC 4 cut(s) 613, 838, 851, 2460
BspQI GCTCTTC 2 cut(s) 372, 2235
BspT107I GGYRCC 2 cut(s) 316, 3391
BspTNI GGTCTC 1 cut(s) 1592
BsrDI GCAATG 3 cut(s) 663, 2079, 2595
BsrGI TGTACA 3 cut(s) 2597, 2754, 2985
BsrI ACTGG 7 cut(s) 302, 349, 525, 695, 2340, 2454, 3033
BssECI CCNNGG 6 cut(s) 1507, 2164, 2165, 2642, 2836, 3364
BssSI CACGAG 1 cut(s) 410
Bst2BI CACGAG 1 cut(s) 410
Bst2UI CCWGG 7 cut(s) 185, 948, 1509, 2166, 2290, 2837, 3365
Bst4CI ACNGT 6 cut(s) 1357, 1527, 2379, 2646, 2935, 3399
Bst6I CTCTTC 6 cut(s) 372, 1485, 1641, 2196, 2235, 2489
BstAPI GCANNNNNTGC 1 cut(s) 674
BstAUI TGTACA 3 cut(s) 2597, 2754, 2985
BstBAI YACGTR 1 cut(s) 3385
BstC8I GCNNGC 5 cut(s) 255, 628, 767, 1068, 3255
BstDEI CTNAG 9 cut(s) 41, 1256, 1994, 2085, 2201, 2423, 2456, 2781, 3134
BstDSI CCRYGG 1 cut(s) 2642
BstENI CCTNNNNNAGG 1 cut(s) 1604
BstF5I GGATG 9 cut(s) 14, 253, 459, 943, 1084, 1228, 1543, 1776, 2944
BstFNI CGCG 2 cut(s) 1239, 2921
BstH2I RGCGCY 1 cut(s) 385
BstHHI GCGC 1 cut(s) 384
BstMAI GTCTC 3 cut(s) 1592, 2520, 3538
BstMWI GCNNNNNNNGC 6 cut(s) 158, 674, 2234, 2243, 2557, 2862
BstNI CCWGG 7 cut(s) 185, 948, 1509, 2166, 2290, 2837, 3365
BstNSI RCATGY 4 cut(s) 592, 1070, 2312, 3300
BstSCI CCNGG 7 cut(s) 183, 946, 1507, 2164, 2288, 2835, 3363
BstSFI CTRYAG 3 cut(s) 88, 170, 1113
BstSLI GKGCMC 3 cut(s) 56, 321, 411
BstUI CGCG 2 cut(s) 1239, 2921
BstV1I GCAGC 3 cut(s) 156, 424, 661
BstV2I GAAGAC 2 cut(s) 1161, 3095
BstX2I RGATCY 3 cut(s) 422, 843, 2452
BstYI RGATCY 3 cut(s) 422, 843, 2452
BsuI GTATCC 3 cut(s) 2438, 3018, 3317
BsuRI GGCC 2 cut(s) 2751, 2830
BtgI CCRYGG 1 cut(s) 2642
BtgZI GCGATG 1 cut(s) 1220
BtrI CACGTC 2 cut(s) 2728, 3548
BtsCI GGATG 9 cut(s) 14, 253, 459, 943, 1084, 1228, 1543, 1776, 2944
BtsIMutI CAGTG 4 cut(s) 1641, 1819, 2931, 3100
BtuMI TCGCGA 1 cut(s) 1239
BveI ACCTGC 1 cut(s) 2169
Cac8I GCNNGC 5 cut(s) 255, 628, 767, 1068, 3255
CciI TCATGA 1 cut(s) 264
CfoI GCGC 1 cut(s) 384
Cfr13I GGNCC 3 cut(s) 904, 2639, 2829
CseI GACGC 1 cut(s) 2818
DdeI CTNAG 9 cut(s) 41, 1256, 1994, 2085, 2201, 2423, 2456, 2781, 3134
Eam1104I CTCTTC 6 cut(s) 372, 1485, 1641, 2196, 2235, 2489
EarI CTCTTC 6 cut(s) 372, 1485, 1641, 2196, 2235, 2489
Ecl136II GAGCTC 3 cut(s) 253, 2560, 3020
Eco24I GRGCYC 4 cut(s) 255, 2562, 3022, 3480
Eco31I GGTCTC 1 cut(s) 1592
Eco32I GATATC 2 cut(s) 790, 1243
Eco47I GGWCC 2 cut(s) 904, 2639
Eco47III AGCGCT 1 cut(s) 383
Eco53kI GAGCTC 3 cut(s) 253, 2560, 3020
Eco57I CTGAAG 7 cut(s) 723, 1665, 1857, 2177, 2251, 3345, 3450
Eco88I CYCGRG 1 cut(s) 2153
EcoICRI GAGCTC 3 cut(s) 253, 2560, 3020
EcoNI CCTNNNNNAGG 1 cut(s) 1604
EcoRI GAATTC 1 cut(s) 111
EcoRII CCWGG 7 cut(s) 183, 946, 1507, 2164, 2288, 2835, 3363
EcoRV GATATC 2 cut(s) 790, 1243
EcoT22I ATGCAT 2 cut(s) 1452, 1543
EcoT38I GRGCYC 4 cut(s) 255, 2562, 3022, 3480
Esp3I CGTCTC 1 cut(s) 3538
FaqI GGGAC 4 cut(s) 1480, 2182, 2853, 3570
FauI CCCGC 2 cut(s) 2628, 3164
FbaI TGATCA 4 cut(s) 79, 486, 1443, 2712
FblI GTMKAC 2 cut(s) 1522, 2479
Fnu4HI GCNGC 7 cut(s) 6, 25, 170, 438, 675, 1758, 2664
FokI GGATG 8 cut(s) 260, 466, 930, 1071, 1235, 1550, 1783, 2931
FriOI GRGCYC 4 cut(s) 255, 2562, 3022, 3480
Fsp4HI GCNGC 7 cut(s) 6, 25, 170, 438, 675, 1758, 2664
FspBI CTAG 4 cut(s) 972, 1403, 1910, 2043
GlaI GCGC 1 cut(s) 383
GluI GCNGC 7 cut(s) 6, 25, 170, 438, 675, 1758, 2664
GsuI CTGGAG 3 cut(s) 2688, 2793, 2819
HaeII RGCGCY 1 cut(s) 385
HaeIII GGCC 2 cut(s) 2751, 2830
HapII CCGG 2 cut(s) 841, 2555
HgaI GACGC 1 cut(s) 2818
HhaI GCGC 1 cut(s) 384
Hin6I GCGC 1 cut(s) 382
HinP1I GCGC 1 cut(s) 382
HincII GTYRAC 2 cut(s) 697, 2184
HindII GTYRAC 2 cut(s) 697, 2184
HindIII AAGCTT 1 cut(s) 1264
HpaI GTTAAC 1 cut(s) 697
HpaII CCGG 2 cut(s) 841, 2555
HphI GGTGA 3 cut(s) 2117, 2307, 2924
HpyCH4III ACNGT 6 cut(s) 1357, 1527, 2379, 2646, 2935, 3399
HpyCH4IV ACGT 8 cut(s) 271, 852, 1516, 2365, 2727, 3184, 3384, 3547
HpyF10VI GCNNNNNNNGC 6 cut(s) 158, 674, 2234, 2243, 2557, 2862
HpyF3I CTNAG 9 cut(s) 41, 1256, 1994, 2085, 2201, 2423, 2456, 2781, 3134
HpySE526I ACGT 8 cut(s) 271, 852, 1516, 2365, 2727, 3184, 3384, 3547
HspAI GCGC 1 cut(s) 382
Kpn2I TCCGGA 1 cut(s) 2554
Ksp22I TGATCA 4 cut(s) 79, 486, 1443, 2712
KspAI GTTAAC 1 cut(s) 697
LguI GCTCTTC 2 cut(s) 372, 2235
LmnI GCTCC 9 cut(s) 149, 2166, 2197, 2557, 2565, 2785, 3017, 3250, 3360
Lsp1109I GCAGC 3 cut(s) 156, 424, 661
LweI GCATC 6 cut(s) 11, 424, 1459, 1528, 1625, 1761
MaeI CTAG 4 cut(s) 972, 1403, 1910, 2043
MaeII ACGT 8 cut(s) 271, 852, 1516, 2365, 2727, 3184, 3384, 3547
MaeIII GTNAC 5 cut(s) 1823, 2014, 2614, 2929, 3331
MfeI CAATTG 2 cut(s) 2172, 3510
MflI RGATCY 3 cut(s) 422, 843, 2452
MhlI GDGCHC 8 cut(s) 56, 255, 321, 411, 2511, 2562, 3022, 3480
MlyI GAGTC 4 cut(s) 1502, 2031, 2485, 2835
MmeI TCCRAC 4 cut(s) 538, 1200, 2665, 3286
Mph1103I ATGCAT 2 cut(s) 1452, 1543
MroI TCCGGA 1 cut(s) 2554
MroXI GAANNNNTTC 3 cut(s) 939, 1663, 2264
MslI CAYNNNNRTG 1 cut(s) 19
MspA1I CMGCKG 3 cut(s) 484, 1757, 2666
MspI CCGG 2 cut(s) 841, 2555
MspR9I CCNGG 7 cut(s) 185, 948, 1509, 2166, 2290, 2837, 3365
MunI CAATTG 2 cut(s) 2172, 3510
MvaI CCWGG 7 cut(s) 185, 948, 1509, 2166, 2290, 2837, 3365
MvnI CGCG 2 cut(s) 1239, 2921
MwoI GCNNNNNNNGC 6 cut(s) 158, 674, 2234, 2243, 2557, 2862
NlaIV GGNNCC 9 cut(s) 318, 525, 845, 2640, 2766, 2831, 3362, 3393, 3518
NmuCI GTSAC 2 cut(s) 1823, 2929
NruI TCGCGA 1 cut(s) 1239
NsiI ATGCAT 2 cut(s) 1452, 1543
NspI RCATGY 4 cut(s) 592, 1070, 2312, 3300
PaeI GCATGC 1 cut(s) 1070
PaeR7I CTCGAG 1 cut(s) 2153
PagI TCATGA 1 cut(s) 264
PasI CCCWGGG 1 cut(s) 2165
PciI ACATGT 1 cut(s) 588
PciSI GCTCTTC 2 cut(s) 372, 2235
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 3457
PdmI GAANNNNTTC 3 cut(s) 939, 1663, 2264
PflMI CCANNNNNTGG 1 cut(s) 1388
PfoI TCCNGGA 3 cut(s) 183, 946, 2288
PkrI GCNGC 7 cut(s) 7, 26, 171, 439, 676, 1759, 2665
PleI GAGTC 4 cut(s) 1501, 2031, 2484, 2835
PpsI GAGTC 4 cut(s) 1501, 2031, 2484, 2835
Ppu21I YACGTR 1 cut(s) 3385
PscI ACATGT 1 cut(s) 588
PshBI ATTAAT 1 cut(s) 2057
PsiI TTATAA 2 cut(s) 681, 965
Psp124BI GAGCTC 3 cut(s) 255, 2562, 3022
Psp1406I AACGTT 1 cut(s) 2365
Psp6I CCWGG 7 cut(s) 183, 946, 1507, 2164, 2288, 2835, 3363
PspGI CCWGG 7 cut(s) 183, 946, 1507, 2164, 2288, 2835, 3363
PspN4I GGNNCC 9 cut(s) 318, 525, 845, 2640, 2766, 2831, 3362, 3393, 3518
PspPI GGNCC 3 cut(s) 904, 2639, 2829
PstI CTGCAG 1 cut(s) 174
PsuI RGATCY 3 cut(s) 422, 843, 2452
PvuII CAGCTG 1 cut(s) 484
RruI TCGCGA 1 cut(s) 1239
RseI CAYNNNNRTG 1 cut(s) 19
SacI GAGCTC 3 cut(s) 255, 2562, 3022
SapI GCTCTTC 2 cut(s) 372, 2235
SatI GCNGC 7 cut(s) 6, 25, 170, 438, 675, 1758, 2664
Sau96I GGNCC 3 cut(s) 904, 2639, 2829
ScaI AGTACT 1 cut(s) 1359
SchI GAGTC 4 cut(s) 1502, 2031, 2485, 2835
ScrFI CCNGG 7 cut(s) 185, 948, 1509, 2166, 2290, 2837, 3365
SduI GDGCHC 8 cut(s) 56, 255, 321, 411, 2511, 2562, 3022, 3480
SfaNI GCATC 6 cut(s) 11, 424, 1459, 1528, 1625, 1761
SfcI CTRYAG 3 cut(s) 88, 170, 1113
Sfr274I CTCGAG 1 cut(s) 2153
SinI GGWCC 2 cut(s) 904, 2639
SlaI CTCGAG 1 cut(s) 2153
SmiMI CAYNNNNRTG 1 cut(s) 19
SmlI CTYRAG 7 cut(s) 232, 1049, 2048, 2111, 2153, 2510, 2954
SmoI CTYRAG 7 cut(s) 232, 1049, 2048, 2111, 2153, 2510, 2954
SphI GCATGC 1 cut(s) 1070
SsiI CCGC 7 cut(s) 25, 554, 1757, 2635, 2664, 2919, 3171
SspI AATATT 3 cut(s) 2746, 2887, 3206
SspMI CTAG 4 cut(s) 972, 1403, 1910, 2043
SstI GAGCTC 3 cut(s) 255, 2562, 3022
StyD4I CCNGG 7 cut(s) 183, 946, 1507, 2164, 2288, 2835, 3363
TaaI ACNGT 6 cut(s) 1357, 1527, 2379, 2646, 2935, 3399
TaiI ACGT 8 cut(s) 274, 855, 1519, 2368, 2730, 3187, 3387, 3550
TaqI TCGA 6 cut(s) 1075, 1156, 1407, 1797, 2154, 3451
TatI WGTACW 6 cut(s) 1123, 1357, 2597, 2754, 2904, 2985
TauI GCSGC 3 cut(s) 27, 1760, 2666
TscAI CASTG 4 cut(s) 1648, 1819, 2938, 3100
TseFI GTSAC 2 cut(s) 1823, 2929
TseI GCWGC 4 cut(s) 5, 169, 437, 674
Tsp45I GTSAC 2 cut(s) 1823, 2929
TspGWI ACGGA 3 cut(s) 1134, 2917, 3456
TspRI CASTG 4 cut(s) 1648, 1819, 2938, 3100
Van91I CCANNNNNTGG 1 cut(s) 1388
VneI GTGCAC 1 cut(s) 407
VpaK11BI GGWCC 2 cut(s) 904, 2639
VspI ATTAAT 1 cut(s) 2057
XagI CCTNNNNNAGG 1 cut(s) 1604
XceI RCATGY 4 cut(s) 592, 1070, 2312, 3300
XhoI CTCGAG 1 cut(s) 2153
XmiI GTMKAC 2 cut(s) 1522, 2479
XmnI GAANNNNTTC 3 cut(s) 939, 1663, 2264
XspI CTAG 4 cut(s) 972, 1403, 1910, 2043
ZrmI AGTACT 1 cut(s) 1359
Zsp2I ATGCAT 2 cut(s) 1452, 1543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.