MD17G1275000.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000654
Physical Location & Seq
Forward (+)
33633738 .. 33636403
2666 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1275000.v1.1.491

Sequence Viewer

Length: 2286 bp
ATGGATTATACAAGTACAATGTTGGACAGCTTAAAGTTTTACACAAGGATTGGGATTGAAAATCTAGTTGACAGATGCCTAGTGAAAATTCAAGGCTCGAACAGGTTGTTGGTCATGCATCAATTGGTGAGAAACATGGCAATGGAAATTATTTGTGAAGAATCACCTGATGACCCTGGAAAACGTAGTAGAGTGTTGCAGAAAGATGCCTCTAATATTTTGAGAAAATTAAGTGGTACGGAAAATATTAAGGGCCTCATGCTTAACCTTGCTAGCAAAATAACGTTTGTTGGATGTCATAAAAAACGATGCCATGATGAAGATTATGATGGAAATTGTTCAAGACGACGTCGTCTTGGTTTCTTCTCTTGGAAGCCAGTTAGCTTTCCGTCCACAAACTCAGCTTCTGCATCAAATGAGATAGATTTCAAATCTGAGGCGTTTAGAAGGATGCACAATCTTGAAATTCTCATGCTCAACAACGTCAACGTCAGGGGAAGCTACGAAGAATTTCCAAAAAGTTTAATATGTTTATCTTGGAGAGGATTCCACTTAAAATCAGTACCAGAAAATTTTTATTTGGAAAACCTGGTTGTTCTTGACATGCGAAATAGTAGCCTACAACATGTTTGGAATGGGATCAGGTTCTTTCCGAGGCTGAAGATTCTTAATCTCAGTCATTCGCATGGCCTTAGGACAACCCCTGACTTCTCAGGACTCCCTAACCTTGAGAGATTGATTCTTAAACATTGCATAAATTTGGTTGAGGTTGATGAGTCCATCGGATACCTAGAGAAACTTGTTGTTTTGAATCTAGAAGGCTGCAAAAATCTCATGAAGCTTCCAAAATTGAGATCTATTCAGGATCTTATTCTTTCTGGTTGCTCAAAGCTTGTGCTTGGTGCCAATACGACTGCCACAACTGGTCACTTACAATCTACAGCTTGTGAATTGAAGAAACTCAATCTGCTATCAGCTAAATCATGGTATTCAATCTGGTCGCGGGTGTCCCCAAGAAAAAATATTGAACCAAGCAGTTTCTCATTGGCAAGTTTACCACATTCCTTGACAAGTTTAAGTCTGGATAGCTGCAATCTATCAGAGATTCCGAGTGCCCTTACTATGCTGTCTTCGTTAGAGTACTTGGATCTGAATAAGAATCCAATTACAAGCCTACCAGAGAGCATGAACAATCTTGTTAAGCTTCACACTCTTGCAGTAGATGGTTGCAGAAACCTCACAATGCTTCCAGAGCTCCCACATAGTTTGAAACAATTGAATGGTAGAGGTTGCACATCATTGAAAAGAATAACAAATTTACCGAACTTGCACATATTACCGGCCACACATCCCTTCGGCATGCAAAAGGACATAGATTTAAAGTATTACAACATATTATTGGACTCAAATTTATGGTATTGTGGGAAACTAGTTGATGTTGTAAGCTTGTTTAATACACTACCATTGAGCAGCTTTGACATAAAACTCCTCAAAGATATCGATCTATTCGATTTGAAACCCAACGGTGGCGTTGAGGTCCAAAGGAACAGCGATTGTGCAAGTTTTGGCAAGCCCAGGCGCTCCAAGGGAACTTTTGAATGCGGCATATTTAGCATATTTGTCCACGAGAGCAAGATTCCAGATCGGTTCAATTACAGGAGCATGGGTAACAGTGTGTTCTCTATTATTGTTCCTTCACAGCCTAATATCAAGATCGTAGGTTTAAATGCATGTATATTGTATGCCCGGCAATCGGATCGAAGCCCTGAGATTGCACTTGGAAACTATCATTTCTATTCGCTTGAACTTAGGAATGAGACAAAGGGCCTTAAGTGGCGTTTACCTATGTTCAATGAGGTTGAATGGAGGGTGATGAACGAAGATATGTTATGGCTAAGCCATTGGATTATGGGGAACGATGAATTGGAATGCGGGGAACAAGTGCGTTTTGAAATAATCGGAGTGCAAGACGGTTTCGTCACAAAGGAGATTGGAGTCCAGTTTGTGTACGAGCAGCAAAATAAGGATAAGGAGGATGTCCCATCAAGTAGTGAAGATCCAATGATCCTACCTGACAGATCGTTTCGGGTCTGGAGCAAAGTATCGCCTTGGCACTGGAGCGTAGGTTTCGCTGGAAGGGGTGGCCCTATTGGGAGAGAGACCGGGGTGGAATACATCCATAACAATGAGAAGAAAGTTATTTCAACTGTCATGTGCGCGGCTCAATACATTCTCTGTAACCATGAAGTGATCGATCTTGAGAATGAATCCCGCATGACCCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

762

Amino Acids

86.57

Weight (kDa)

8.36

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 2 - 55 1.4e-06 Disease resistance protein Roq1-like, winged-helix domain
LRR_8 PF13855 355 - 410 3.9e-07 Leucine rich repeat
LRR_14 PF23598 356 - 446 1.5e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000654)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g20301 FvH4_2g20310 FvH4_2g20310 FvH4_2g20700
malus_domestica MD07G1079200.v1.1 MD07G1079300.v1.1 MD07G1079400.v1.1 MD12G1024400.v1.1 MD15G1375000.v1.1 MD15G1375200.v1.1 MD15G1375500.v1.1 MD15G1376100.v1.1 MD15G1376200.v1.1 MD15G1376300.v1.1 MD17G1274300.v1.1 MD17G1275000.v1.1 MD17G1275400.v1.1 MD17G1275900.v1.1 MD17G1276000.v1.1 MD17G1276600.v1.1 MD17G1277700.v1.1
prunus_persica Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.2G101900_v2.0.a1 Prupe.3G009700_v2.0.a1 Prupe.3G010100_v2.0.a1 Prupe.3G010300_v2.0.a1 Prupe.3G130700_v2.0.a1 Prupe.3G130800_v2.0.a1 Prupe.3G130900_v2.0.a1
pyrus_communis pycom07g06290 pycom07g06320 pycom15g33580 pycom15g33620 pycom17g27240 pycom17g27360 pycom17g27390 pycom17g27400 pycom17g27410 pycom17g27450 pycom17g27530
rosa_chinensis RchiOBHm_Chr4g0405381 RchiOBHm_Chr6g0286441
rosa_laevigata RLG00000008845 RLG00000012572
rosa_multiflora Rmu_sc0000575.1_g000003 Rmu_sc0003585.1_g000001 Rmu_sc0027178.1_g000001 Rmu_sc0042065.1_g000001
rosa_roxburghii Rroxscaffold_4G00313580 Rroxscaffold_5G00349860 Rroxscaffold_7G00182360
rosa_rugosa Rorug01G0140300.1 Rorug01G0140400.1 Rorug04G0057900 Rorug04G0057900 Rorug04G0058000 Rorug04G0058100 Rorug04G0058200 Rorug06G0179200 Rorug06G0184100
rosa_samantha Rh4AG131500 Rh4BG126200 Rh4BG126300 Rh4CG138700 Rh4DG125600 Rh6AG292000 Rh6BG295500 Rh6BG300700 Rh6CG296200 Rh6DG288000
rosa_wichuraiana Rw1G013150 Rw1G013160 Rw4G010650 Rw6G025170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 351, 1976
AatII GACGTC 1 cut(s) 352
AccB1I GGYRCC 1 cut(s) 902
AccII CGCG 2 cut(s) 1003, 2219
AciI CCGC 5 cut(s) 1003, 1602, 1932, 2219, 2272
AclI AACGTT 1 cut(s) 284
AclWI GGATC 6 cut(s) 647, 873, 1155, 1764, 2051, 2059
AcoI YGGCCR 1 cut(s) 1341
AcsI RAATTY 7 cut(s) 87, 465, 509, 571, 757, 1315, 1408
AcuI CTGAAG 1 cut(s) 680
AcyI GRCGYC 1 cut(s) 349
AfaI GTAC 5 cut(s) 16, 238, 564, 1142, 2009
AfiI CCNNNNNNNGG 2 cut(s) 1526, 1753
AflII CTTAAG 1 cut(s) 1829
AflIII ACRYGT 1 cut(s) 625
AhlI ACTAGT 1 cut(s) 1429
AjnI CCWGG 3 cut(s) 175, 588, 1574
AjuI GAANNNNNNNTTGG 4 cut(s) 92, 124, 1907, 1939
Alw21I GWGCWC 1 cut(s) 1257
Alw26I GTCTC 2 cut(s) 1811, 2153
AlwI GGATC 6 cut(s) 647, 873, 1155, 1764, 2051, 2059
AlwNI CAGNNNCTG 1 cut(s) 407
AoxI GGCC 5 cut(s) 253, 688, 1341, 1825, 2143
ApeKI GCWGC 4 cut(s) 822, 1089, 1470, 2014
ApoI RAATTY 7 cut(s) 87, 465, 509, 571, 757, 1315, 1408
Asp700I GAANNNNTTC 2 cut(s) 337, 510
AspLEI GCGC 2 cut(s) 1581, 2219
AspS9I GGNCC 4 cut(s) 253, 1537, 1825, 2144
AsuC2I CCSGG 2 cut(s) 1747, 2164
AsuHPI GGTGA 3 cut(s) 139, 156, 1882
AsuNHI GCTAGC 1 cut(s) 272
AvaII GGWCC 1 cut(s) 1537
AxyI CCTNAGG 1 cut(s) 692
BaeGI GKGCMC 1 cut(s) 1117
BanI GGYRCC 1 cut(s) 902
BanII GRGCYC 1 cut(s) 1257
BauI CACGAG 1 cut(s) 1625
BbsI GAAGAC 1 cut(s) 1122
Bbv12I GWGCWC 1 cut(s) 1257
BbvI GCAGC 4 cut(s) 809, 1076, 1482, 2026
BccI CCATC 4 cut(s) 323, 788, 1217, 2050
BcgI CGANNNNNNTGC 2 cut(s) 1739, 1773
BciT130I CCWGG 3 cut(s) 177, 590, 1576
BciVI GTATCC 1 cut(s) 779
BcnI CCSGG 2 cut(s) 1747, 2164
BcoDI GTCTC 2 cut(s) 1811, 2153
BcuI ACTAGT 1 cut(s) 1429
BfaI CTAG 6 cut(s) 65, 80, 273, 791, 815, 1430
BfmI CTRYAG 1 cut(s) 939
BfoI RGCGCY 1 cut(s) 1582
BfrI CTTAAG 1 cut(s) 1829
BfuI GTATCC 1 cut(s) 779
BglII AGATCT 1 cut(s) 854
BisI GCNGC 6 cut(s) 823, 1090, 1471, 1603, 2015, 2220
BlpI GCTNAGC 1 cut(s) 1895
BlsI GCNGC 6 cut(s) 824, 1091, 1472, 1604, 2016, 2221
BmcAI AGTACT 1 cut(s) 1142
Bme1390I CCNGG 5 cut(s) 177, 590, 1576, 1747, 2164
Bme18I GGWCC 1 cut(s) 1537
BmgT120I GGNCC 4 cut(s) 253, 1537, 1825, 2144
BmiI GGNNCC 1 cut(s) 904
BmrFI CCNGG 5 cut(s) 177, 590, 1576, 1747, 2164
BmsI GCATC 6 cut(s) 65, 127, 196, 299, 419, 441
BmtI GCTAGC 1 cut(s) 276
BpiI GAAGAC 1 cut(s) 1122
BpmI CTGGAG 2 cut(s) 2113, 2137
Bpu1102I GCTNAGC 1 cut(s) 1895
BpuEI CTTGAG 2 cut(s) 749, 2279
BpuMI CCSGG 2 cut(s) 1747, 2164
Bsa29I ATCGAT 2 cut(s) 1500, 2253
BsaBI GATNNNNATC 1 cut(s) 1500
BsaHI GRCGYC 1 cut(s) 349
BsaI GGTCTC 1 cut(s) 2153
BsaJI CCNNGG 6 cut(s) 175, 653, 1574, 1584, 2108, 2163
BsaXI ACNNNNNCTCC 4 cut(s) 1651, 1681, 2110, 2140
Bsc4I CCNNNNNNNGG 2 cut(s) 1526, 1753
Bse118I RCCGGY 1 cut(s) 1339
Bse1I ACTGG 4 cut(s) 377, 928, 1999, 2120
Bse21I CCTNAGG 1 cut(s) 692
Bse3DI GCAATG 2 cut(s) 147, 748
Bse8I GATNNNNATC 1 cut(s) 1500
BseBI CCWGG 3 cut(s) 177, 590, 1576
BseCI ATCGAT 2 cut(s) 1500, 2253
BseDI CCNNGG 6 cut(s) 175, 653, 1574, 1584, 2108, 2163
BseGI GGATG 5 cut(s) 299, 456, 1348, 2041, 2175
BseJI GATNNNNATC 1 cut(s) 1500
BseLI CCNNNNNNNGG 2 cut(s) 1526, 1753
BseMI GCAATG 2 cut(s) 147, 748
BseMII CTCAG 5 cut(s) 414, 426, 688, 726, 1758
BseNI ACTGG 4 cut(s) 377, 928, 1999, 2120
BseRI GAGGAG 1 cut(s) 1478
BseSI GKGCMC 1 cut(s) 1117
BseXI GCAGC 4 cut(s) 809, 1076, 1482, 2026
Bsh1236I CGCG 2 cut(s) 1003, 2219
BshFI GGCC 5 cut(s) 255, 690, 1343, 1827, 2145
BshNI GGYRCC 1 cut(s) 902
BshVI ATCGAT 2 cut(s) 1500, 2253
BsiHKAI GWGCWC 1 cut(s) 1257
BsiSI CCGG 3 cut(s) 1340, 1747, 2163
BslFI GGGAC 2 cut(s) 994, 2024
BslI CCNNNNNNNGG 2 cut(s) 1526, 1753
BsmAI GTCTC 2 cut(s) 1811, 2153
BsmFI GGGAC 2 cut(s) 994, 2024
BsmI GAATGC 2 cut(s) 1604, 1934
BsnI GGCC 5 cut(s) 255, 690, 1343, 1827, 2145
Bso31I GGTCTC 1 cut(s) 2153
Bsp1286I GDGCHC 2 cut(s) 1117, 1257
Bsp1720I GCTNAGC 1 cut(s) 1895
BspACI CCGC 5 cut(s) 1003, 1602, 1932, 2219, 2272
BspANI GGCC 5 cut(s) 255, 690, 1343, 1827, 2145
BspCNI CTCAG 5 cut(s) 413, 427, 687, 725, 1759
BspDI ATCGAT 2 cut(s) 1500, 2253
BspFNI CGCG 2 cut(s) 1003, 2219
BspHI TCATGA 1 cut(s) 834
BspLI GGNNCC 1 cut(s) 904
BspOI GCTAGC 1 cut(s) 276
BspPI GGATC 6 cut(s) 647, 873, 1155, 1764, 2051, 2059
BspT107I GGYRCC 1 cut(s) 902
BspTI CTTAAG 1 cut(s) 1829
BspTNI GGTCTC 1 cut(s) 2153
BsrDI GCAATG 2 cut(s) 147, 748
BsrFI RCCGGY 1 cut(s) 1339
BsrI ACTGG 4 cut(s) 377, 928, 1999, 2120
BssAI RCCGGY 1 cut(s) 1339
BssECI CCNNGG 6 cut(s) 175, 653, 1574, 1584, 2108, 2163
BssNI GRCGYC 1 cut(s) 349
BssSI CACGAG 1 cut(s) 1625
BssT1I CCWWGG 2 cut(s) 1584, 2108
Bst2BI CACGAG 1 cut(s) 1625
Bst2UI CCWGG 3 cut(s) 177, 590, 1576
Bst4CI ACNGT 4 cut(s) 1526, 1673, 1973, 2209
BstACI GRCGYC 1 cut(s) 349
BstAFI CTTAAG 1 cut(s) 1829
BstC8I GCNNGC 3 cut(s) 274, 1361, 1571
BstDEI CTNAG 8 cut(s) 400, 435, 674, 692, 712, 1767, 1808, 1895
BstF5I GGATG 5 cut(s) 299, 456, 1348, 2041, 2175
BstFNI CGCG 2 cut(s) 1003, 2219
BstH2I RGCGCY 1 cut(s) 1582
BstHHI GCGC 2 cut(s) 1581, 2219
BstMAI GTCTC 2 cut(s) 1811, 2153
BstMWI GCNNNNNNNGC 2 cut(s) 1252, 1611
BstNI CCWGG 3 cut(s) 177, 590, 1576
BstNSI RCATGY 4 cut(s) 607, 629, 1363, 1734
BstSCI CCNGG 5 cut(s) 175, 588, 1574, 1745, 2162
BstSFI CTRYAG 1 cut(s) 939
BstSLI GKGCMC 1 cut(s) 1117
BstUI CGCG 2 cut(s) 1003, 2219
BstV1I GCAGC 4 cut(s) 809, 1076, 1482, 2026
BstV2I GAAGAC 1 cut(s) 1122
BstX2I RGATCY 4 cut(s) 854, 865, 1147, 2056
BstYI RGATCY 4 cut(s) 854, 865, 1147, 2056
Bsu15I ATCGAT 2 cut(s) 1500, 2253
Bsu36I CCTNAGG 1 cut(s) 692
BsuI GTATCC 1 cut(s) 779
BsuRI GGCC 5 cut(s) 255, 690, 1343, 1827, 2145
BsuTUI ATCGAT 2 cut(s) 1500, 2253
BtsCI GGATG 5 cut(s) 299, 456, 1348, 2041, 2175
BtsIMutI CAGTG 2 cut(s) 1678, 2113
Cac8I GCNNGC 3 cut(s) 274, 1361, 1571
CaiI CAGNNNCTG 1 cut(s) 407
CciI TCATGA 1 cut(s) 834
CfoI GCGC 2 cut(s) 1581, 2219
Cfr10I RCCGGY 1 cut(s) 1339
Cfr13I GGNCC 4 cut(s) 253, 1537, 1825, 2144
ClaI ATCGAT 2 cut(s) 1500, 2253
CsiI ACCWGGT 1 cut(s) 588
Csp6I GTAC 5 cut(s) 15, 237, 563, 1141, 2008
CspCI CAANNNNNGTGG 4 cut(s) 1047, 1082, 1248, 1283
CviQI GTAC 5 cut(s) 15, 237, 563, 1141, 2008
DdeI CTNAG 8 cut(s) 400, 435, 674, 692, 712, 1767, 1808, 1895
DraI TTTAAA 2 cut(s) 1380, 1725
DrdI GACNNNNNNGTC 2 cut(s) 351, 1976
DseDI GACNNNNNNGTC 2 cut(s) 351, 1976
EaeI YGGCCR 1 cut(s) 1341
Ecl136II GAGCTC 1 cut(s) 1255
Eco130I CCWWGG 2 cut(s) 1584, 2108
Eco24I GRGCYC 1 cut(s) 1257
Eco31I GGTCTC 1 cut(s) 2153
Eco32I GATATC 1 cut(s) 1498
Eco47I GGWCC 1 cut(s) 1537
Eco53kI GAGCTC 1 cut(s) 1255
Eco57I CTGAAG 1 cut(s) 680
Eco81I CCTNAGG 1 cut(s) 692
EcoICRI GAGCTC 1 cut(s) 1255
EcoO109I RGGNCCY 2 cut(s) 253, 1825
EcoRII CCWGG 3 cut(s) 175, 588, 1574
EcoRV GATATC 1 cut(s) 1498
EcoT14I CCWWGG 2 cut(s) 1584, 2108
EcoT22I ATGCAT 2 cut(s) 120, 1732
EcoT38I GRGCYC 1 cut(s) 1257
ErhI CCWWGG 2 cut(s) 1584, 2108
FaqI GGGAC 2 cut(s) 994, 2024
FauI CCCGC 3 cut(s) 996, 1925, 2279
Fnu4HI GCNGC 6 cut(s) 823, 1090, 1471, 1603, 2015, 2220
FokI GGATG 5 cut(s) 306, 463, 1335, 2048, 2162
FriOI GRGCYC 1 cut(s) 1257
Fsp4HI GCNGC 6 cut(s) 823, 1090, 1471, 1603, 2015, 2220
FspBI CTAG 6 cut(s) 65, 80, 273, 791, 815, 1430
GlaI GCGC 2 cut(s) 1580, 2218
GluI GCNGC 6 cut(s) 823, 1090, 1471, 1603, 2015, 2220
GsuI CTGGAG 2 cut(s) 2113, 2137
HaeII RGCGCY 1 cut(s) 1582
HaeIII GGCC 5 cut(s) 255, 690, 1343, 1827, 2145
HapII CCGG 3 cut(s) 1340, 1747, 2163
HhaI GCGC 2 cut(s) 1581, 2219
Hin1I GRCGYC 1 cut(s) 349
Hin6I GCGC 2 cut(s) 1579, 2217
HinP1I GCGC 2 cut(s) 1579, 2217
HincII GTYRAC 2 cut(s) 70, 487
HindII GTYRAC 2 cut(s) 70, 487
HindIII AAGCTT 4 cut(s) 839, 890, 1202, 1444
HpaII CCGG 3 cut(s) 1340, 1747, 2163
HphI GGTGA 3 cut(s) 139, 156, 1882
Hpy166II GTNNAC 7 cut(s) 70, 393, 487, 1055, 1624, 1841, 2008
Hpy188I TCNGA 8 cut(s) 436, 654, 785, 1102, 1110, 1152, 1756, 1961
Hpy8I GTNNAC 7 cut(s) 70, 393, 487, 1055, 1624, 1841, 2008
Hpy99I CGWCG 2 cut(s) 351, 354
HpyAV CCTTC 5 cut(s) 441, 812, 1363, 1704, 2130
HpyCH4III ACNGT 4 cut(s) 1526, 1673, 1973, 2209
HpyCH4IV ACGT 5 cut(s) 184, 284, 349, 483, 489
HpyF10VI GCNNNNNNNGC 2 cut(s) 1252, 1611
HpyF3I CTNAG 8 cut(s) 400, 435, 674, 692, 712, 1767, 1808, 1895
HpySE526I ACGT 5 cut(s) 184, 284, 349, 483, 489
Hsp92I GRCGYC 1 cut(s) 349
HspAI GCGC 2 cut(s) 1579, 2217
LmnI GCTCC 5 cut(s) 1260, 1586, 1659, 2094, 2118
Lsp1109I GCAGC 4 cut(s) 809, 1076, 1482, 2026
LweI GCATC 6 cut(s) 65, 127, 196, 299, 419, 441
MabI ACCWGGT 1 cut(s) 588
MaeI CTAG 6 cut(s) 65, 80, 273, 791, 815, 1430
MaeII ACGT 5 cut(s) 184, 284, 349, 483, 489
MaeIII GTNAC 4 cut(s) 926, 1667, 1978, 2237
MfeI CAATTG 2 cut(s) 122, 1274
MflI RGATCY 4 cut(s) 854, 865, 1147, 2056
MhlI GDGCHC 2 cut(s) 1117, 1257
MlyI GAGTC 4 cut(s) 711, 785, 1397, 2004
MmeI TCCRAC 1 cut(s) 271
Mph1103I ATGCAT 2 cut(s) 120, 1732
MroXI GAANNNNTTC 2 cut(s) 337, 510
MslI CAYNNNNRTG 3 cut(s) 140, 684, 2184
MspCI CTTAAG 1 cut(s) 1829
MspI CCGG 3 cut(s) 1340, 1747, 2163
MspR9I CCNGG 5 cut(s) 177, 590, 1576, 1747, 2164
MunI CAATTG 2 cut(s) 122, 1274
Mva1269I GAATGC 2 cut(s) 1604, 1934
MvaI CCWGG 3 cut(s) 177, 590, 1576
MvnI CGCG 2 cut(s) 1003, 2219
MwoI GCNNNNNNNGC 2 cut(s) 1252, 1611
NciI CCSGG 2 cut(s) 1747, 2164
NheI GCTAGC 1 cut(s) 272
NlaIV GGNNCC 1 cut(s) 904
NmuCI GTSAC 2 cut(s) 926, 1978
NsiI ATGCAT 2 cut(s) 120, 1732
NspI RCATGY 4 cut(s) 607, 629, 1363, 1734
PaeI GCATGC 1 cut(s) 1363
PagI TCATGA 1 cut(s) 834
PciI ACATGT 1 cut(s) 625
PcsI WCGNNNNNNNCGW 1 cut(s) 1506
PctI GAATGC 2 cut(s) 1604, 1934
PdmI GAANNNNTTC 2 cut(s) 337, 510
PfeI GAWTC 9 cut(s) 161, 546, 664, 739, 811, 1105, 1159, 1636, 2267
PflFI GACNNNGTC 2 cut(s) 348, 351
PkrI GCNGC 6 cut(s) 824, 1091, 1472, 1604, 2016, 2221
PleI GAGTC 4 cut(s) 711, 784, 1397, 2003
PpsI GAGTC 4 cut(s) 711, 784, 1397, 2003
PscI ACATGT 1 cut(s) 625
Psp124BI GAGCTC 1 cut(s) 1257
Psp1406I AACGTT 1 cut(s) 284
Psp6I CCWGG 3 cut(s) 175, 588, 1574
PspGI CCWGG 3 cut(s) 175, 588, 1574
PspN4I GGNNCC 1 cut(s) 904
PspPI GGNCC 4 cut(s) 253, 1537, 1825, 2144
PstNI CAGNNNCTG 1 cut(s) 407
PsuI RGATCY 4 cut(s) 854, 865, 1147, 2056
PsyI GACNNNGTC 2 cut(s) 348, 351
RsaI GTAC 5 cut(s) 16, 238, 564, 1142, 2009
RsaNI GTAC 5 cut(s) 15, 237, 563, 1141, 2008
RseI CAYNNNNRTG 3 cut(s) 140, 684, 2184
SacI GAGCTC 1 cut(s) 1257
SatI GCNGC 6 cut(s) 823, 1090, 1471, 1603, 2015, 2220
Sau96I GGNCC 4 cut(s) 253, 1537, 1825, 2144
ScaI AGTACT 1 cut(s) 1142
SchI GAGTC 4 cut(s) 711, 785, 1397, 2004
ScrFI CCNGG 5 cut(s) 177, 590, 1576, 1747, 2164
SduI GDGCHC 2 cut(s) 1117, 1257
SexAI ACCWGGT 1 cut(s) 588
SfaNI GCATC 6 cut(s) 65, 127, 196, 299, 419, 441
SfcI CTRYAG 1 cut(s) 939
SinI GGWCC 1 cut(s) 1537
SmiMI CAYNNNNRTG 3 cut(s) 140, 684, 2184
SmlI CTYRAG 3 cut(s) 728, 1829, 2258
SmoI CTYRAG 3 cut(s) 728, 1829, 2258
SpeI ACTAGT 1 cut(s) 1429
SphI GCATGC 1 cut(s) 1363
SsiI CCGC 5 cut(s) 1003, 1602, 1932, 2219, 2272
SspI AATATT 3 cut(s) 217, 247, 1024
SspMI CTAG 6 cut(s) 65, 80, 273, 791, 815, 1430
SstI GAGCTC 1 cut(s) 1257
StyD4I CCNGG 5 cut(s) 175, 588, 1574, 1745, 2162
StyI CCWWGG 2 cut(s) 1584, 2108
TaaI ACNGT 4 cut(s) 1526, 1673, 1973, 2209
TaiI ACGT 5 cut(s) 187, 287, 352, 486, 492
TaqI TCGA 5 cut(s) 98, 1500, 1509, 1759, 2253
TatI WGTACW 2 cut(s) 14, 1140
TauI GCSGC 2 cut(s) 1605, 2222
TfiI GAWTC 9 cut(s) 161, 546, 664, 739, 811, 1105, 1159, 1636, 2267
TscAI CASTG 2 cut(s) 1678, 2120
TseFI GTSAC 2 cut(s) 926, 1978
TseI GCWGC 4 cut(s) 822, 1089, 1470, 2014
Tsp45I GTSAC 2 cut(s) 926, 1978
TspDTI ATGAA 7 cut(s) 333, 851, 1202, 1889, 1935, 2259, 2280
TspGWI ACGGA 2 cut(s) 254, 378
TspRI CASTG 2 cut(s) 1678, 2120
Tth111I GACNNNGTC 2 cut(s) 348, 351
Vha464I CTTAAG 1 cut(s) 1829
VpaK11BI GGWCC 1 cut(s) 1537
XapI RAATTY 7 cut(s) 87, 465, 509, 571, 757, 1315, 1408
XbaI TCTAGA 1 cut(s) 814
XceI RCATGY 4 cut(s) 607, 629, 1363, 1734
XmnI GAANNNNTTC 2 cut(s) 337, 510
XspI CTAG 6 cut(s) 65, 80, 273, 791, 815, 1430
ZraI GACGTC 1 cut(s) 350
ZrmI AGTACT 1 cut(s) 1142
Zsp2I ATGCAT 2 cut(s) 120, 1732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.